Thermo Scientific MSC-Advantage User manual

Valid:
Operating Instructions
Biological Safety Cabinet
MSC-Advantage™
Valid: 04.2011 / 50128856

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
50128856_04.2011
Inhaltsverzeichnis
Copyright2011
These operating instructions are protected by copyright. Rights resulting
thereof, particularly reprint, photomechanical or digital postprocessing or
reproduction, even in part, are only allowed with the written consent of
Thermo Electron LED GmbH.
This regulation does not apply to reproductions for in-plant use.
Trademarks
MSCAdvantageand SmartFlowareregisteredtrademarks ofThermoScientific.
Thermo Scientific is a brand of Thermo Fisher Scientific Inc.
All other trademarks mentioned in the operating instructions are the exclusive
property of the respective manufacturers.
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Germany
The Thermo Electron LED GmbH is a subsidiaryfrom:
Thermo Fisher Scientific Inc.
81 Wyman Street
Waltham, MA 02454
USA

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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Contents
1 General notes .........................................................................................6
1.1 Instruction of the operating personnel ...........................................6
1.2 Applicability of the instructions ......................................................7
1.3 Warranty.........................................................................................7
1.4 Explanation of symbols..................................................................8
1.4.1 Symbols used in the operating instructions..............................8
1.4.2 Symbols on the device .............................................................9
1.5 Use of the device .........................................................................10
1.5.1 Correct use .............................................................................10
1.5.2 Incorrect use ...........................................................................10
1.6 Standards and safety regulations ................................................ 11
2 Delivery .................................................................................................12
2.1 Standard components..................................................................12
2.2 Acceptance inspection.................................................................12
2.3 Transport security lock and device packaging ............................12
3 Installation ............................................................................................13
3.1 Ambient conditions ......................................................................13
3.2 Room ventilation ..........................................................................14
3.3 Correct location............................................................................14
3.4 Installation in series .....................................................................14
3.5 Transport......................................................................................15
4 Description of the device ....................................................................16
4.1 Overall view .................................................................................16
4.2 Safety system ..............................................................................18
4.3 Filter system.................................................................................19
4.4 Operating and display elements ..................................................20
4.5 Sample chamber access .............................................................21
4.6 Device interfaces .........................................................................22
4.7 Sample chamber illumination ......................................................24
4.8 UV lamp unit ................................................................................24
4.9 Working area................................................................................24
5 Start-up..................................................................................................25
5.1 Initial operation.............................................................................25
5.2 Installing the device and accessories ..........................................25
5.3 Unlocking the transport protection...............................................26
5.4 Levelling the cabinet ....................................................................26
5.5 Power supply connection.............................................................27
5.6 Installation test .............................................................................29
6 Handling and control ...........................................................................30
6.1 Operating panel ...........................................................................30
6.1.1 Functional units.......................................................................30
6.1.2 Display during calibration routine ...........................................31
6.1.3 Display during UV disinfection................................................31
6.1.4 Failure messages ...................................................................32
6.2 Device start-up.............................................................................32
6.3 Description of the operating modes.............................................33
7 Operation ..............................................................................................35
7.1 Hygiene preparations for the sample chamber ...........................35
7.2 Loading the sample chamber ......................................................35
7.3 Response to failure messages ....................................................35
7.4 Work rules ....................................................................................36

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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Figures
Fig. 1 Devicearrangement in the operating room ..................... 14
Fig. 2 Lift points........................................................................15
Fig. 3 Overallview.....................................................................17
Fig. 4 Filter system with downflow and exhaust air filter ..........19
Fig. 5 Operating and display elements ..................................... 20
Fig. 6 Samplechamberopening...............................................21
Fig. 7 Supplyinterfaces............................................................22
Fig. 8 UV lamp unit ..................................................................24
Fig. 9 Working area on the workplate, armrests....................... 24
Fig. 10 Stand installation ............................................................ 25
Fig. 11 Unlocking the transport protection .................................26
Fig. 12 OperatingpanelI ............................................................30
Fig. 13 OperatingpanelII ...........................................................31
Fig. 14 Display upon start-up .....................................................32
Fig. 15 Sitting posture ................................................................ 36
Fig. 16 Protectivegridsegment..................................................42
Fig. 17 UV lamp replacement..................................................... 44
Contents
8 Shut-down ............................................................................................. 37
8.1 Interruptinganoperation ...............................................................37
8.2 Shuttingthe device down .............................................................. 37
9 Cleaning and decontamination.......................................................... 38
9.1 Decontaminationprocedure..........................................................38
9.2 Wipe/spraydisinfection ................................................................38
9.3 UV disinfection after a wipe/spray disinfection .............................40
9.3.1 UV disinfection using the integral UV lamp .............................40
9.3.2 UV disinfection using a mobile UV device (optional) ............... 40
9.3.3 Changing UV disinfection time ................................................40
9.4 Disinfectionwithformaldehyde .....................................................41
9.5 Cleaning the exterior surfaces ...................................................... 41
9.6 Cleaningthefrontwindow.............................................................41
9.7 Cleaningthefloorpan ....................................................................42
9.8 Cleaning the protectivegrid ..........................................................42
10 Maintenance ......................................................................................... 43
10.1 Inspection .....................................................................................43
10.2 Service.......................................................................................... 43
10.2.1 Sample chamber illumination .................................................. 44
10.2.2 Optional UV lamps ..................................................................44
10.3 Retrofitting and repairs ............................................................... 44
10.4 ExchangingSmartPorts................................................................45
11 Disposal ................................................................................................. 46
11.1 Disposalprocedure......................................................................46
12 Technical data ...................................................................................... 48
13 Device log ............................................................................................. 52
14 Certificate of decontamination ........................................................... 53

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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1 General notes
The following are the addresses of the international Thermo
FisherScientific Sales Organisations.
Postal address Germany
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Inquiries from Germany
Phone
Phone Sales 0800 1 536376
Phone Service 0800 1 112110
Fax Sales/Service 0800 1 112114
EMail [e-mail]
Enquiries from Europe, Middle East and Africa
Phone + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-6772
EMail [e-mail]
Postal address USA
Thermo Fisher Scientific
275Aiken Road
Asheville, NC 28804
USA
Enquiries from North America
Phone [phone]
Fax [phone]
EMail [e-mail]
Enquiries from Latin America
Phone [phone]
Fax [phone]
EMail [e-mail]
Enquiries from Asia Pacific
Phone [phone]
Fax [phone]
EMail [e-mail]

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
50128856_04.2011
1 General notes
1.1 Data of the MSC-Advantage and the documentation
Device identification
Device Name: Biological Safety Cabinet
Model: MSC-Advantage ™
Model lines: 0.9, 1.2, 1.5, 1.8
Model 1.2.from serial devices: 41070608
Model 1.8.from serial devices: 41070639
Assignment of the product documentation
User manual: 501268856
Availability: 04.2011
Certification and Quality Audit:
Conformity: CE conformity marking
1.2 Instruction of the operating personnel
These operating instructions describe the biological safety cabinet
• MSCAdvantage
and apply to the models MSC 0.9, 1.2, 1.5, 1.8.
Thebiologicalsafetycabinethasbeenmanufacturedinkeepingwiththelatesttech-
nological developmentsandhasbeentested beforedeliveryfor itscorrectfunction.
Itmay, however, present potentialhazards if itis not used according to the intended
purpose or outside of operating parameters. Therefore, the following procedures
must always be observed to prevent accidents:
• The biological safetycabinet must be operated onlyby trained and authorized
personnel.
• For anyoperation of this device, the operator must prepare clear and concise
written instructions in the language of the operating and cleaningpersonnel ba-
sedontheseoperatinginstructions,applicablesafetydatasheets,planthygiene
guidelines, and technical regulations, in particular:
• which decontamination measures are to be applied for the cabinet and
accessories,
• which protective measures applywhile specific agents are used,
• which measures are to be taken in the case of an accident.
• Repairstothe device must be carried out onlybytrained andauthorized expert
personnel.
1.3 Applicability of the instructions
• The contents of the operating instructions are subject to change without
further notice.
• Concerning translationsinto foreign languages, the German version of these
operating instructions is binding.
• Keep theseoperating instructions close to thedevice so thatsafetyinstructions
and important information are always accessible.
• Should you encounter problems that are not detailed adequatelyin these ope-
ratinginstructions,please contactThermoFisherScientificimmediatelyforyour
own safety.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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1 General notes
1.4 Warranty
Thermo Fisher Scientific warrants the operationalsafety and functions of the bi-
ological safety cabinet only under the condition that:
• the device is operated and serviced exclusivelyin accordance with its intended
purpose and as described in these operating instructions,
• the device is not modified,
• onlyoriginal spare parts and accessories thathave been approved byThermo
Fisher Scientific are used,
• inspections and maintenance are performed at the specified intervals,
• an installation test is performed prior to the initial operation of the device and
that a repeat test is performed on the occasion of all inspections and repairs.
The warrantyis valid from the date of deliveryof the device to the operator.
1.5 Standards and safety regulations
The device complies with the safetyrequirements of the following standards and
directives:
• EN 12469
• NF 095 Rev3.2006 / NF - Postes de Sécurité Microbiologique
• IEC 61010-1
• EN 61010-1
• EN 12469
• DINEN 61326-1
• Low Voltage Directive 2006/95 EG
• EMC Directive 2004/108 EG
For the setting-up and installation of the biological safetycabinet the respective
national regulations must be observed.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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1 General notes
1.6 Explanation of symbols
1.6.1 Symbols used in the operating instructions
WARNING!
is used if non-observance may cause serious or even lethal
injuries.
CAUTION!
is used if non-observance may cause medium tominor injuries
or damage.
NOTE!
is used for hints and useful information.
RECYCLING!
Valuable raw materials can be reused.
Warning against electric shock.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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1 General notes
1.6.2 Symbols on the device
Observe operating instructions (switchbox ceiling)
Warning against hand injuries (device side walls)
Biohazard (left front section of device)
CE Conformity declaration
Checked safety (cover light dome)
Norme Française / NF - Postes de Sécurité Microbiologique
2 x T 5A
T5A note (sample chamber fusing)
RS 232 interface (top side of plenum panel)
Installion of the armrests (right side of the light hood)

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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1 General notes
1.7 Use of the device
1.7.1 Correct use
The Biological SafetyCabinet is alaboratory device for installation and operation
in microbiological and biotechnical laboratoriesof safetylevels 1,2, and 3. Ithas
beendesignedasaClassIImicrobiologicalbiologicalsafetycabinet,inaccordance
with EN 12469.
Depending on the hazard level of the agents involved, the operator must prepare
in writing appropriate decontamination procedures forthe device andthe acces-
sories used in the sample chamber.
Prior totheinitialoperationof the cabinet, the operator must perform an installation
test. The test result must be documented bya test report. The cabinet must only
be released for operation if it is in compliancewith the operating parameters spe-
cified by Thermo Fisher Scientific.
After any changes to the installation conditions and after any modification to the
technical system, a repeat test must be performed and the test result mustbe do-
cumented by a test report that shows that all operating parameters are in com-
pliance with those specified by Thermo Fisher Scientific.
1.7.2 Incorrect use
The biological safety cabinet must not be used in laboratories that do not comply
with the requirements of safety levels 1, 2 or 3.
The device must not be operated as a Class II biological safety cabinet if:
• no repeat test is performed after changes to the installation conditions or after
modifications to the technical system,
• the alarm system of the device has issued a failure message and the cause
for the failure has notbeen repaired.
The alarm system must notbe tampered with or disabled. If alarm system com-
ponents hevebeen removed ordisabledforserviceorrepairs,the devicemustonly
be released for operation if all alarm system components are functioning properly
again.
The filters installed in the device are not capable of separating gaseous sub-
stances. Therefore, never store or process gases or gas-releasing substances in
the device:
• which in quantity or concentration are toxic,
• if a reactionwith othersubstancesmay result in hazardous toxic concentrations
or formation of toxic gases,
• that may form combustible or explosive mixtures in combination with air.

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2 Delivery
2.1 Scope of delivery
Deliveryfor the biological safety cabinet includes the following:
• biological safety cabinet (without stand)
• armrests
• device documentation:
– operating instructions
– factory test report
Optional components and accessories are listed as separate items in the deli-
very document.
2.2 Acceptance inspection
After the device has been delivered, immediately check the device:
• for completeness,
• for possible damage.
If the delivery is incomplete or if you detect any transport damage to the
device, contact the forwarding agency and Thermo Fisher Scientific im-
mediately.
2.3 Transport security lock and device packaging
A transport security lock protects the device counterweight during transport.
Protective packagings protect the floorpan, the front window, and the workplate
segments. To remove the transport security lock and the protective packagings,
please refer to the enclosed installation instructions and to Sections 5.2 and 5.3
of these instructions.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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3 Installation
3.1 Ambient conditions
The operationalsafety and correct function of the device depends on the location
where it is to be operated. The biological safetycabinet must be operated onlyat
locations that meet the ambient conditions listed below.
Location requirements:
• The electrical system of thedevice has been designed for an operating height
of up to 2000 m above sea level.
• The mains power supply outlet should be out of casual reach to prevent ac-
cidental shut-off. Ideally, the outlet should be installed above the safety
cabinet.
The outlet must be accessible to authorized personnel only. It constitutes,
together with the power cable plug, the disconnection device for all poles.
• The flooring of the location must be adequately strong and not flammable.
• The stand must ensure a sufficient load-bearing capacity (twice the device
weight).
• The room in which the device is installed must be of adequate height. For
devices that are not connected to an exhaust system, the distance between
the exhaust air opening at the device ceiling and the room ceiling must be
at least 200 mm (8 in).
• The location must be equipped with an appropriate ventilation system
(see Section 3.2.).
• For the valves maybe installed a lateral distance of at least 300 mm is required
to ensure accessibility for the installation, see Chapter 4.6
• The temperaturewithintheroommustbebetween15 °C and 40 °C
(49 °F and 104 °F).
• The relative humidity in the vicinity of the device must not exceed 90 %.
NOTE - Ambient conditions!
If ambient conditions vary from those descri-
bed above, please contact Thermo Fisher
Scientific forassistance in installingthe device.
NOTE - Temporary storage!
If the device is stored only temporarily (up to
four weeks), the ambient temperature may be
between -20 °C and +60 °C (-4 °F and +140 °F)
at a relative air humidity of up to 90 %. For lon-
gerstorage periods, the location requirements
apply.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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3 Installation
3.2 Room ventilation
The room ventilation should preferably be a ventilation system that complies with
the national requirements for the application.
• The inlet air and exhaust air openings of the room ventilation must be located
so that drafts are prevented from impairing the function of the safety cabinet
air system.
Coupling to laboratory exhaust systems
Coupling biological safety cabinets to a laboratory exhaust system, air that spills
of biological agents must be discharged to prevent inflow to the installation space.
The ventilationsystem of the biological safetycabinet itself arenottobe influenced.
If a coupling to a laboratoryexhaust system is installed, it must be ensured that
noharmfulairflowiscreatedintheinstallationspace.The volumeofairdischarged
by the exhaust system must be tracked accordingly into the installation space.
It should be noted, that the equipment air flow itself is not affected.
The on site exhaust system should be dimensioned, so the air velocity scale in
thedraftinterruptorandexhaust air plenum is sufficient, thattheequipment exhaust
air has no air resistance.
Instates,where the coupling to a laboratoryexhaust system is regulated byfurther
provisions, therelevant national regulations must be observed.
CAUTION – Installation test!
During the initial start-up of the biological
safety cabinet an initial installation test must
be performed.
The installation location of the biological safe-
ty cabinet with connection to a technical ven-
tilation or with a coupling to laboratory
exhaut system and additional installedexhaust
accessories are not to be changed.
Every modification of the installation location
requires a new installation test (see chapter
5.6)!

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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3 Installation
3.3 Correct location
Choose a draft-free location where thebiological safetycabinet
does not interfere with the plant traffic.
Fig. 1: Thisfigureshowspreferredlocationsforbiologicalsafety
cabinets and unsuitable locations that are not in accordance
with the safety requirements.
Unsuitable locations: The locations [1], [2], and [3] are not
suitable because they are exposed to drafts from windows
and doors.
Location [5] is unsuitable because it is within range of plant
traffic and within the exhaust air range of a ventilation system
[4].
Preferred locations: The locations [6], [7], and [8] are correct
because theyare in adraft-freesection of the room and not ex-
posed to plant traffic.
Acounterweightatthedevice backpanelmovessynchronously
with the vertical movement of the front window. To prevent the
counterweight from jamming, the device backpanel should be
as close to the wall as possible.
3.4 Installation in series
When several devices are to be installed in series,please ob-
serve the following:
• Make sure that vibrations cannot be transferred
between adjacent devices.
• Exterior surfaces of the cabinets must always be ac-
cessible for cleaning and disinfection.
1 2
3
4
6
7
8
5
Fig. 1
Device arrangement in the
operating room

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
3 Installation
3.5 Transport
Fig. 2:To prevent tilting, always transport the device usinga sui-
table carrier, even for a transport within a building, and separate
it from the stand (see Section 5.2).
CAUTION – Tilting danger!
If the unit is tilted too much during lifting,
risk of tipping exists. Lift the biological safety
cabinet only
vertically!
For transportation (including inside buildings)
use an appropriate lifting device, which
ensures that the unit:
• is on a stable stand and
• is secured against lateral tilting.
Do not transport the biological safety cabinet
on a base with roles.
CAUTION – Lift points!
For transport, lift the device only at the lift
points shown in Figure 2.
Do not allow the weight of the cabinet to rest
on the floorpan!
CAUTION – Crushing hazard!
The weight of the front window is counterba-
lanced bythe counterweight (at the rear of the
device).
The device must not be transported unless the
counterweight has been locked.
• Install the four lockscrews (see Section 5.3).
Fig. 2
Lift points

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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4 Description of the device
4.1 Overall view
•Fig. 3: Plenum assembly [3] with plenum for downflow blower [19] and plenum
for exhaust air blower [20]. The downflowfilter and the exhaustairfilter are in-
stalled immediatelyto the pertaining plenum. The exhaust air is discharged to
the exterior of the device through an opening.
The plenum assembly is concealed behind a cover [18].
• Switchbox [2] with power supply unit and power supply cable [1]. The top
of the device contains an RS 232 connection [23] for a PC and two fuse hol-
ders [22].
• Light dome [4] for the sample chamber illumination unit, equipped with one
fluorescent tube (model MSC 1.2) or two fluorescent tubes (model MSC 0.9,
1.5, 1.8).
The optional, device-integral UV lamp is installed to the ceiling at the front
section of the sample chamber.
• Operating panel [5] with function keys and indicators.
• Front window [7] with two handles [6].
• Bushings [8] and [9] in the side panels (3 on each side). The bushings [9] can
be used to install media valves [11], the bushings [8] of type SmartPort are
provided for laying cables or hoses into the utilitychamber.
• Stand [10] (optional).
• Workplate segments [13] with 2 armrests [14].Aone-piece workplate and spe-
cial workplates are available as optional accessories.
• Internal outlets [15] for thepower supply of accessories (optionally, one of the
outlets can be equipped with an adapter [17] for mobile UV devices).
• Test hoses for the downflow unit [16] atthe left side of the samplechamberand
for the exhaust air unit [12] at the right side of the sample chamber.
NOTE – Test hoses!
Do not remove the caps of the two test hoses
for checking downflow and exhaust air.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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4 Description of the device
Fig. 3
Overall view

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
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4 Description of the device
4.2 Safety system
The safety system comprises a combination of protective and alarm systems
that ensure maximum personal and material protection.
Safety systems:
•Vacuum-sealed air system
A vacuum-sealed air system in combination with HEPA filters for downflow
and exhaust air forms the basis of the safety system for personal and ma-
terial protection.
• Personal protection
Air aspired from the exterior along the entire working opening at a constant
high velocity prevents:
• agents leaking through the working opening of the chamber.
As the exterior air pressure around the device exceeds the pressure of the
internal air system (vacuum sealing), it ensures:
• agents cannot be released to the exterior in the case of a leak in the
cabinet housing.
• Material protection
A steady airflow within the air system ensures:
• a constant downflow allowing the HEPA filters to remove contaminants
so that the samples are always surrounded by ultrapure air,
• harmful particles are not carried over through the sample chamber
(protection from cross-contamination).
•HEPA filters
The downflow (i.e. the air circulating within the device) and the exhaust air
(air that is released to the exterior) are cleaned byHEPAfilters (HEPA= High
Efficiency Particulate Air Filter).
• Safety lockout
To protect from UV radiation, the optional UV disinfection routine can be run
only if the front opening is closed. During UV disinfection, the front opening
safety lockout is activated and prevents harmful UV radiation from being
emitted from the sample chamber.
Circuits can be driven via potential-free contacts (monitor contacts), e.g. a
solenoid that switches automatically upon cancellation of the work mode.
Warning system:
• Airflow monitoring
Airflowmonitoring determinesthe velocityof theairflowin the sample chamber
as well as the inflow velocity of the air aspired from the exterior through the
working opening.As soon as airflow velocities move above or below a spe-
cified safety value, a signal is transmitted to the alarm system.
• Visual and audible alarm system
The warning system constantlymonitors the safety-relevant device functions:
• Inflow velocity of the air aspired from the exterior,
• downflow velocity,
• work position of the front window.
If the warning system detects changes to one of these device func-
tions, it issues:
• an audible and a visual alarm signal.

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Operating Instructions Biological Safety Cabinet | MSC-Advantage
4 Description of the device
•Position monitoring
The position sensors detect the opening state of the front
window and indicate whether the window is open or closed
in the work position.
•SmartFlow Indicatordisplay
The SmartFlowIndicator displays the compensation capa-
billity of the exhaust control, if the window is in working po-
sition and blower on.
4.3 Filter system
Fig. 4: The filter system consists of two HEPAfilters [5]and [2]
for the device downflow and exhaust air.
HEPA filters: Room air [10] is drawn into the sample cham-
ber through the working opening. In the air duct, room air and
the downflow within the chamber [7] are then blended to make
up the blend air [8]. The blend air is then:
• filtered proportionally by the downflow filter [5] and supp-
lied as ultrapure air [6] evenly into the sample chamber of
the device,
• filtered bythe exhaust air filter [2] and released as ultrapure
air [1] to the exterior of the device.
Inlet air protection: Multi-segment protective grids [9] are in-
stalled below the work surface in the air duct between sample
chamber and device plenum. The grids prevent coarse particles
from entering the plenum where theymight impair the function
of the blowers [3] [4] and of the filters [2] [5]. The grids can be
removed for cleaning.
Fig. 4
Filter system with downflow
and exhaust air filter

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4 Description of the device
4.4 Operating and display elements
Fig.5: Thebiological safetycabinet isoperatedusinganoperatingpanelconsisting
of keys, function indicators and a display.
[A] The display with its5-digit indicator panel shows the followinginformation, re-
gardless of the activated operating function:
– normal operation: operating hours of the device,
– safe work mode: downflow and exhaust air velocities,
– calibration routine: status of the calibration function,
– device-controlled UV disinfection (optional): remaining disinfection
time.
Values are output as integers.
[B]Keys for switching operational functions on or off,
[C]Status indicators show the operational status of:
– front window work position,
– airflow,
– reduced blower speed,
– SmartFlow Indicator
Fig. 5
Operating and display
elements