Thermo Scientific Vanquish VH-D10 User manual

thermoscientific
Vanquish
Diode Array Detectors
VH-D10
Operating Manual
4820.8301-EN Revision 3.0 •January 2023
*4820.8301-EN-3.0*

Copyright © 2023 Thermo Fisher Scientific Inc. All rights reserved.
Original Operating Manual
The hardware descriptions in this manual revision refer to devices: VH-D10-A.
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Diode Array Detectors (VH-D10)
Operating Manual
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Contacting Us
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Operating Manual

Contents
Diode Array Detectors (VH-D10)
Operating Manual
Page 5
Contents
1 Using this Manual....................................................................... 11
1.1 About this Manual ............................................................................................... 12
1.2 Conventions ......................................................................................................... 13
1.2.1 Conventions for Safety Messages ............................................................ 13
1.2.2 Special Notices ......................................................................................... 13
1.2.3 Typographical Conventions ...................................................................... 14
1.3 Reference Documentation................................................................................... 15
2 Safety......................................................................................... 17
2.1 Safety Symbols and Signal Words ........................................................................ 18
2.1.1 Safety Symbols and Signal Words in this Manual..................................... 18
2.1.2 Observing this Manual ............................................................................. 18
2.1.3 Safety Symbols on the Device .................................................................. 19
2.1.4 Rating Plate .............................................................................................. 19
2.2 Intended Use........................................................................................................ 20
2.3 Safety Precautions ............................................................................................... 21
2.3.1 General Safety Information...................................................................... 21
2.3.2 Qualification of the Personnel ................................................................. 22
2.3.3 Personal Protective Equipment................................................................ 22
2.3.4 Electrical Safety Precautions .................................................................... 23
2.3.5 General Residual Hazards......................................................................... 24
2.3.6 In Case of Emergency ............................................................................... 26
2.4 Solvent and Additive Information........................................................................ 27
2.4.1 General Compatibility .............................................................................. 27
2.4.2 Allowed pH Ranges................................................................................... 27
2.4.3 Allowed Concentrations ........................................................................... 28
2.4.4 Further Information ................................................................................. 28
2.5 Compliance Information ...................................................................................... 29
3 Device Overview......................................................................... 31
3.1 Detector Features ................................................................................................ 32
3.2 Operating Principle .............................................................................................. 33
3.3 Interior Components............................................................................................ 35
3.4 Flow Cell............................................................................................................... 36
3.5 Lamp .................................................................................................................... 38

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3.6 Leak Detection ..................................................................................................... 39
3.7 Operation............................................................................................................. 40
4 Unpacking .................................................................................. 41
4.1 Unpacking ............................................................................................................ 42
4.2 Scope of Delivery ................................................................................................. 44
5 Installation ................................................................................. 45
5.1 Safety Guidelines for Installation......................................................................... 46
5.2 Installing the Device............................................................................................. 47
5.3 Site Requirements................................................................................................ 49
5.3.1 Power Considerations .............................................................................. 49
5.3.2 Power Cord............................................................................................... 49
5.3.3 Condensation ........................................................................................... 50
5.4 Accessing the Interior Components..................................................................... 51
5.5 Setting Up the Hardware ..................................................................................... 52
5.5.1 System Arrangement................................................................................ 52
5.5.2 Connecting the Device ............................................................................. 53
5.5.3 Connecting the Power Cord ..................................................................... 54
5.6 Installing the Flow Cell ......................................................................................... 55
5.7 Setting Up the Flow Connections......................................................................... 59
5.7.1 General Information and Guidelines........................................................ 59
5.7.2 Guiding Capillaries and Tubing Through the System................................ 60
5.7.3 Installing the Partition Panel Plugs........................................................... 62
5.7.4 Connecting Fittings, Capillaries, and Tubing ............................................ 64
5.7.5 Flow Connections to the Flow Cell ........................................................... 66
5.7.6 Guiding Liquid Leaks to Waste ................................................................. 70
5.8 Determining the Pressure inside the Flow Cell .................................................... 71
5.8.1 Measuring the Backpressure of the Waste Line....................................... 73
5.8.2 Measuring the Vanquish System Backpressure (Without Flow Cell and
Additional Module) .................................................................................. 74
5.8.3 Determine the Backpressure at the Flow Cell Outlet Caused by the
Connected Additional Module (Without Flow Cell) ................................. 76
5.8.4 Determining the Backpressure of the Flow Cell ....................................... 78
5.8.5 Calculating the Pressure at the Inlet Port of the Flow Cell....................... 79
5.9 Turning On the Device ......................................................................................... 81
5.10 Setting Up the Device in the Software................................................................. 82

Contents
Diode Array Detectors (VH-D10)
Operating Manual
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6 Operation................................................................................... 83
6.1 Introduction to this Chapter ................................................................................ 84
6.2 Safety Guidelines for Operation........................................................................... 85
6.3 Control Elements ................................................................................................. 86
6.3.1 Keypad...................................................................................................... 86
6.3.2 Status Indicators....................................................................................... 87
6.4 Power On/Off Control.......................................................................................... 89
6.5 Preparing the Device for Operation..................................................................... 90
6.6 Turning On the UV Lamp...................................................................................... 92
6.7 Guidelines for Use of Flow Cells........................................................................... 93
6.8 Important Operating Parameters ........................................................................ 96
6.9 Optimizing the Performance of the Device........................................................ 100
6.9.1 Guidelines for Optimum Performance ................................................... 100
6.9.2 Overview of Optimization Parameters................................................... 101
6.9.3 Selecting the Data Collection Rate ......................................................... 101
6.9.4 Selecting the Response Time and Peak Width ....................................... 102
6.9.5 Optimizing the Bandwidth and Slit Width Settings ................................ 102
6.9.6 Selecting the Reference Wavelength and Bandwidth............................ 104
6.10 Shutting Down the Device ................................................................................. 106
6.10.1 Short-Term Shutdown (Interruption of Operation) ............................... 106
6.10.2 Long-Term Shutdown............................................................................. 107
6.10.3 Restart after Long-Term Shutdown........................................................ 109
7 Maintenance and Service ......................................................... 111
7.1 Introduction to Maintenance and Service ......................................................... 112
7.2 Safety Guidelines for Maintenance and Service ................................................ 113
7.2.1 General................................................................................................... 113
7.2.2 Flow Cells................................................................................................ 115
7.3 General Rules for Maintenance and Service...................................................... 116
7.4 Routine and Preventive Maintenance ............................................................... 117
7.4.1 Maintenance Plan .................................................................................. 117
7.4.2 Cleaning or Decontaminating the Device............................................... 117
7.4.3 Predictive Performance.......................................................................... 119
7.5 Performing a Wavelength Validation and Wavelength Calibration ................... 120
7.6 Replacing the Lamp............................................................................................ 122

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7.7 Flow Cell............................................................................................................. 126
7.7.1 Preparing the Flow Cell for Storage ....................................................... 126
7.7.2 Removing the Flow Cell.......................................................................... 129
7.7.3 Installing the Flow Cell ........................................................................... 130
7.7.4 Back-Flushing the Flow Cell.................................................................... 134
7.8 Diagnostic Cell.................................................................................................... 142
7.8.1 Installing the Diagnostic Cell .................................................................. 142
7.8.2 Removing the Diagnostic Cell................................................................. 143
7.9 Replacing the Waste Line................................................................................... 145
7.10 Replacing the Main Power Fuses ....................................................................... 147
7.11 Updating the Device Firmware .......................................................................... 149
7.12 Replacing the Doors ........................................................................................... 151
7.13 Transporting or Shipping the Device.................................................................. 153
7.13.1 Preparing the Device for Transport........................................................ 153
7.13.2 Transporting the Device to a New Location ........................................... 154
7.13.3 Shipping the Device................................................................................ 155
7.14 Replacing the Slide-In Module ........................................................................... 156
7.14.1 Removing the Slide-In Module ............................................................... 156
7.14.2 Returning the Slide-In Module ............................................................... 158
7.14.3 Installing the Slide-In Module ................................................................ 158
7.14.4 Setting Up the Slide-In Module .............................................................. 160
8 Troubleshooting ....................................................................... 161
8.1 General Information about Troubleshooting..................................................... 162
8.2 Messages............................................................................................................ 164
8.3 Checking the Flow Cell ....................................................................................... 169
8.4 Resolving Liquid Leaks........................................................................................ 170
9 Specifications ........................................................................... 171
9.1 Performance Specifications ............................................................................... 172
9.1.1 Detector Specifications .......................................................................... 172
9.1.2 Flow Cell Specifications .......................................................................... 174
9.2 Physical Specifications ....................................................................................... 175
10 Accessories, Consumables and Replacement Parts ................... 177
10.1 General Information .......................................................................................... 178
10.2 Ship Kit ............................................................................................................... 179

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Diode Array Detectors (VH-D10)
Operating Manual
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10.3 Optional Accessories.......................................................................................... 180
10.4 Consumables and Replacement Parts................................................................ 181
11 Appendix.................................................................................. 183
11.1 Compliance Information .................................................................................... 184
11.1.1 Declarations of Conformity .................................................................... 184
11.1.2 WEEE Compliance .................................................................................. 185
11.1.3 FCC Compliance...................................................................................... 185
11.1.4 NIST Compliance .................................................................................... 185
11.1.5 Manual Release History.......................................................................... 186
11.2 UV Cutoff Wavelengths of Solvents ................................................................... 187
Index ........................................................................................ 189

Contents
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Operating Manual