Thermo Scientific Cryostat Microm Cryo-Star HM 560 User manual

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
1
Thermo Scientific
Microtome Cryostat
Microm Cryo-Star HM 560
INSTRUCTION MANUAL

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
2

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
3
CERTIFICATION
Thermo Fisher Scientific Microm International GmbH certifies that this instrument
has been tested and checked carefully. Its technical data was verified before
shipment to be in accordance with the published specifications.
The instrument complies with applicable international safety regulations.
WARRANTY
This Thermo Fisher Scientific product is warranted against defects in material and
workmanship for a period of 1 year. Parts which prove to be defective during the
warranty period will be repaired or replaced free of charge by the manufacturer. No
other warranty is expressed or implied. Unauthorized modification or repair by third
party persons will void the warranty.
The warranty will expire in case of improper or wrong use of the instrument and in
case the warning and precautionary messages are not observed. Thermo Fisher
Scientific is not liable for any occurring damage.
Errors and omissions excepted. Subject to amendment and improvement without
further notice.
This instruction manual will be supplied together with each instrument. Further
copies can be ordered at the nearest Thermo Fisher Scientific sales office by giving
the serial number of the instrument, the number of the instruction manual and the
date of issue.
This instruction manual is available in the following languages:
Cat. No.
German: 387 490
English: 387 500
French: 387 510

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
4
INTENDED USE
Dear valued Customer,
Thank you for buying this Thermo Fisher Scientific instrument.
Before putting the instrument into operation, please read these operating
instructions carefully to familiarize you with its proper operation and
functions.
Only skilled or specially trained personnel must operate the microtome
cryostat HM 560, i.e. clamping the specimen, trimming, sectioning and taking
off the sections from the instrument. The listed and marked safety measures
as well as the regulations and hygiene measures of your respective lab must
strictly be observed.
Serial No.: __________________
Please check the Serial No. on the type plate, which is placed on the rear
side of your instrument and enter this number here. This way, questions and
service can be handled faster.
Instruction Manual No. 387500
Issued on February 03
rd
, 2009
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Strasse 49
69190 Walldorf
Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]om
Internet: www.ap-walldorf.com

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
5
Intended Use
Table of Contents
Safety Precautions
PART 1 INTRODUCTION
1-1 Description of the Cryo-Star HM 560
1-2 Technical data Cryo-Star HM 560
PART 2 OPERATING INSTRUCTIONS
2-1 Setting up the cryostat
2-2 Initial turn-on
2-3 Basic operational rules
2-4 Setting section thickness and trimming thickness
2-5 Specimen and knife cooling
2-5-1 Actual and set value of the specimen temperature
2-5-2 Actual and set value of the knife temperature
2-5-3 Function fast freezing
2-5-4 Programming temperature parameters
2-6 Cutting movement and retraction
2-7 Feed
2-7-1 Knife carrier coarse feed
2-7-2 Speed of the knife carrier coarse feed
2-7-3 Automatic approach system
2-7-4 Trimming and first cuts
2-7-5 Fine feed
2-8 Setting the cutting window
2-9 Hand wheel brake
2-10 Detaching the specimen
2-11 Indication of cutting processes
2-11-1 Section counter
2-11-2 Section thickness sum
2-11-3 Remaining travel to front end position
2-11-4 Real time
2-12 Setting the real time, WAKE time and ACTIVE time
2-12-1 Setting the real time
2-12-2 Setting the WAKE time
2-12-3 Setting the ACTIVE time

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
6
2-13 Defrosting
2-13-1 Setting the defrost time
2-13-2 Defrosting cycle
2-13-3 Interrupting a defrosting cycle
2-14 Turning off the function retraction
2-15 Customer-specific settings
2-15-1 Setting the contrast
2-15-2 Selecting the language
2-16 Turning off the cooling function
2-17 Illumination of the cooling chamber
2-18 Adjusting the operating panel
2-19 Specimen holder, freezing-on and specimen orientation
2-19-1 Specimen holder, freezing-on
2-19-2 Specimen orientation
2-20 Knife carriers
2-20-1 Standard knife carrier SM
2-20-2 Disposable blade holder SE
2-20-3 Modification from standard knife carrier SM to disposable blade holder SE
2-21 Error code indication
2-21-1 Definition of the error codes
2-22 Accessories
2-22-1 Standard equipment
2-22-2 Additional equipment
PART 3 Theory of operation
3-1 Specimen and knife carrier cooling, defrost cycle
3-2 Cutting movement
3-3 Knife carrier coarse feed and trimming stages
3-4 Automatic approach system
PART 4 Working with the cryostat
4-1 Preparations on the microtome and inside the cooling chamber
4-2 Freezing on the specimen
4-4 Orientation and trimming of specimens
4-5 Sectioning and taking off sections
4-6 How to avoid malfunctions

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
7
PART 5 Maintenance of the cryostat
5-1 Shutting-off for cleaning
5-2 Cleaning and care of the microtome
5-3 Changing the fluorescent lamp
5-4 Disposal of the instrument after final shutdown

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
8
SAFETY PRECAUTIONS
WARNING SIGNALS AND SYMBOLS
The installation and routine use of the Cryo-Star HM 560 is easy and safe if the instructions in this manual are
being observed.
However, the situations which might be risky for the personnel or equipment are specially marked in this
manual with the following symbols and messages.
Note:
Special instructions regarding operation of the instrument.
Caution:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.
Danger:
Special warning messages to prevent harm to persons and/or serious damage to equipment. For
your own safety, please observe these instructions carefully.
Biohazard:
Warning of biological danger.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European countries with a
separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
9
SAFETY PRECAUTIONS
A T T E N T I O N !
The operator's safety is affected, when the instrument is not operated in
accordance with this instruction manual.
Please observe the following general precautions during operation of this
instrument. Failure to comply with these precautions violates safety standards and
the intended use of the instrument. Thermo Fisher Scientific is not liable for misuse
of the instruments and failure to comply with basic safety requirements.
INSTRUMENT GROUNDING
To avoid injury from electrical current, the instrument must be connected with the safety ground. The
instrument is equipped with a three wire ground plug. The power outlet must be connected to the safety
ground and must meet the International Electrotechnical Commission (IEC) regulations.
CAUTION: MAINS VOLTAGE
Never remove instrument covers during operation. Component replacements as well as adjustments must
only be made by trained service personnel. Unplug the unit before removing or opening the covers.
DANGER IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable gases.
HAZARD OF FROSTBITE
Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at
unprotected hands and arms.
HAZARD OF RADIOACTIVE RADIATION
When working with radioactive specimens observe all applicable radiation safety procedures. When working
with radioactive contaminated material, appropriate safety and disinfection measures must be carried out.
According to the rules and regulations concerning the handling of radioactive contaminated material of the
respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
10
HAZARD OF INFECTION
Use the appropriate safety and disinfection measures when working with infectious specimens.
According to the rules and regulations concerning the handling of infectious/radioactive contaminated
material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must
be worn.
HAZARD OF BIOLOGICAL DANGER
Specimens used during the intended operation of the instrument might potentially be infectious. For this
reason, it is recommended to observe the general laboratory regulations concerning protection against
danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be read in the
Laboratory Biosafety Manual : 1984 of the World Health Organization.
CAUTION: DANGER OF SQUEEZING
When placing the microtome cryostat HM 560 onto the height adjustable stage, it is absolutely necessary that
the stage is in its upper position or in case of a mechanical stage that the rear wall will be removed. Not
observing this leads to the risk of squeezing your hands between the rear carrying handles and the stage
housing.
HAZARD OF MALFUNCTION
To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic
environment. This means, that transmitters such as mobile phones must not be operated in their close
vicinity.
CARE IN USING MICROTOME KNIFE
To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting
specimen and knife. If possible, the specimen should be clamped in before the knife is inserted
into the knife holder. Before changing the knife holder, always remove blade or knife! Unused
knives should always be kept in a knife case. Never place the knife with the cutting edge upwards.
Never try to catch a dropping knife!! Never check the sharpness of the cutting edge with your fingers. The
cutting edge is extremely sharp!
WASTE DISPOSAL
All debris, waste as well as infectious and radioactive contaminated material from operation must be
disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning liquids as well
as section waste must be disposed of according to the respective regulations for special waste!