Thermo Scientific Cryostat Microm Cryo-Star HM 560 User manual

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
1
Thermo Scientific
Microtome Cryostat
Microm Cryo-Star HM 560
INSTRUCTION MANUAL

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
2

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
3
CERTIFICATION
Thermo Fisher Scientific Microm International GmbH certifies that this instrument
has been tested and checked carefully. Its technical data was verified before
shipment to be in accordance with the published specifications.
The instrument complies with applicable international safety regulations.
WARRANTY
This Thermo Fisher Scientific product is warranted against defects in material and
workmanship for a period of 1 year. Parts which prove to be defective during the
warranty period will be repaired or replaced free of charge by the manufacturer. No
other warranty is expressed or implied. Unauthorized modification or repair by third
party persons will void the warranty.
The warranty will expire in case of improper or wrong use of the instrument and in
case the warning and precautionary messages are not observed. Thermo Fisher
Scientific is not liable for any occurring damage.
Errors and omissions excepted. Subject to amendment and improvement without
further notice.
This instruction manual will be supplied together with each instrument. Further
copies can be ordered at the nearest Thermo Fisher Scientific sales office by giving
the serial number of the instrument, the number of the instruction manual and the
date of issue.
This instruction manual is available in the following languages:
Cat. No.
German: 387 490
English: 387 500
French: 387 510

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
4
INTENDED USE
Dear valued Customer,
Thank you for buying this Thermo Fisher Scientific instrument.
Before putting the instrument into operation, please read these operating
instructions carefully to familiarize you with its proper operation and
functions.
Only skilled or specially trained personnel must operate the microtome
cryostat HM 560, i.e. clamping the specimen, trimming, sectioning and taking
off the sections from the instrument. The listed and marked safety measures
as well as the regulations and hygiene measures of your respective lab must
strictly be observed.
Serial No.: __________________
Please check the Serial No. on the type plate, which is placed on the rear
side of your instrument and enter this number here. This way, questions and
service can be handled faster.
Instruction Manual No. 387500
Issued on February 03
rd
, 2009
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Strasse 49
69190 Walldorf
Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]om
Internet: www.ap-walldorf.com

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
5
Intended Use
Table of Contents
Safety Precautions
PART 1 INTRODUCTION
1-1 Description of the Cryo-Star HM 560
1-2 Technical data Cryo-Star HM 560
PART 2 OPERATING INSTRUCTIONS
2-1 Setting up the cryostat
2-2 Initial turn-on
2-3 Basic operational rules
2-4 Setting section thickness and trimming thickness
2-5 Specimen and knife cooling
2-5-1 Actual and set value of the specimen temperature
2-5-2 Actual and set value of the knife temperature
2-5-3 Function fast freezing
2-5-4 Programming temperature parameters
2-6 Cutting movement and retraction
2-7 Feed
2-7-1 Knife carrier coarse feed
2-7-2 Speed of the knife carrier coarse feed
2-7-3 Automatic approach system
2-7-4 Trimming and first cuts
2-7-5 Fine feed
2-8 Setting the cutting window
2-9 Hand wheel brake
2-10 Detaching the specimen
2-11 Indication of cutting processes
2-11-1 Section counter
2-11-2 Section thickness sum
2-11-3 Remaining travel to front end position
2-11-4 Real time
2-12 Setting the real time, WAKE time and ACTIVE time
2-12-1 Setting the real time
2-12-2 Setting the WAKE time
2-12-3 Setting the ACTIVE time

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
6
2-13 Defrosting
2-13-1 Setting the defrost time
2-13-2 Defrosting cycle
2-13-3 Interrupting a defrosting cycle
2-14 Turning off the function retraction
2-15 Customer-specific settings
2-15-1 Setting the contrast
2-15-2 Selecting the language
2-16 Turning off the cooling function
2-17 Illumination of the cooling chamber
2-18 Adjusting the operating panel
2-19 Specimen holder, freezing-on and specimen orientation
2-19-1 Specimen holder, freezing-on
2-19-2 Specimen orientation
2-20 Knife carriers
2-20-1 Standard knife carrier SM
2-20-2 Disposable blade holder SE
2-20-3 Modification from standard knife carrier SM to disposable blade holder SE
2-21 Error code indication
2-21-1 Definition of the error codes
2-22 Accessories
2-22-1 Standard equipment
2-22-2 Additional equipment
PART 3 Theory of operation
3-1 Specimen and knife carrier cooling, defrost cycle
3-2 Cutting movement
3-3 Knife carrier coarse feed and trimming stages
3-4 Automatic approach system
PART 4 Working with the cryostat
4-1 Preparations on the microtome and inside the cooling chamber
4-2 Freezing on the specimen
4-4 Orientation and trimming of specimens
4-5 Sectioning and taking off sections
4-6 How to avoid malfunctions

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
7
PART 5 Maintenance of the cryostat
5-1 Shutting-off for cleaning
5-2 Cleaning and care of the microtome
5-3 Changing the fluorescent lamp
5-4 Disposal of the instrument after final shutdown

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
8
SAFETY PRECAUTIONS
WARNING SIGNALS AND SYMBOLS
The installation and routine use of the Cryo-Star HM 560 is easy and safe if the instructions in this manual are
being observed.
However, the situations which might be risky for the personnel or equipment are specially marked in this
manual with the following symbols and messages.
Note:
Special instructions regarding operation of the instrument.
Caution:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.
Danger:
Special warning messages to prevent harm to persons and/or serious damage to equipment. For
your own safety, please observe these instructions carefully.
Biohazard:
Warning of biological danger.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European countries with a
separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
9
SAFETY PRECAUTIONS
A T T E N T I O N !
The operator's safety is affected, when the instrument is not operated in
accordance with this instruction manual.
Please observe the following general precautions during operation of this
instrument. Failure to comply with these precautions violates safety standards and
the intended use of the instrument. Thermo Fisher Scientific is not liable for misuse
of the instruments and failure to comply with basic safety requirements.
INSTRUMENT GROUNDING
To avoid injury from electrical current, the instrument must be connected with the safety ground. The
instrument is equipped with a three wire ground plug. The power outlet must be connected to the safety
ground and must meet the International Electrotechnical Commission (IEC) regulations.
CAUTION: MAINS VOLTAGE
Never remove instrument covers during operation. Component replacements as well as adjustments must
only be made by trained service personnel. Unplug the unit before removing or opening the covers.
DANGER IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable gases.
HAZARD OF FROSTBITE
Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at
unprotected hands and arms.
HAZARD OF RADIOACTIVE RADIATION
When working with radioactive specimens observe all applicable radiation safety procedures. When working
with radioactive contaminated material, appropriate safety and disinfection measures must be carried out.
According to the rules and regulations concerning the handling of radioactive contaminated material of the
respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
10
HAZARD OF INFECTION
Use the appropriate safety and disinfection measures when working with infectious specimens.
According to the rules and regulations concerning the handling of infectious/radioactive contaminated
material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must
be worn.
HAZARD OF BIOLOGICAL DANGER
Specimens used during the intended operation of the instrument might potentially be infectious. For this
reason, it is recommended to observe the general laboratory regulations concerning protection against
danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be read in the
Laboratory Biosafety Manual : 1984 of the World Health Organization.
CAUTION: DANGER OF SQUEEZING
When placing the microtome cryostat HM 560 onto the height adjustable stage, it is absolutely necessary that
the stage is in its upper position or in case of a mechanical stage that the rear wall will be removed. Not
observing this leads to the risk of squeezing your hands between the rear carrying handles and the stage
housing.
HAZARD OF MALFUNCTION
To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic
environment. This means, that transmitters such as mobile phones must not be operated in their close
vicinity.
CARE IN USING MICROTOME KNIFE
To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting
specimen and knife. If possible, the specimen should be clamped in before the knife is inserted
into the knife holder. Before changing the knife holder, always remove blade or knife! Unused
knives should always be kept in a knife case. Never place the knife with the cutting edge upwards.
Never try to catch a dropping knife!! Never check the sharpness of the cutting edge with your fingers. The
cutting edge is extremely sharp!
WASTE DISPOSAL
All debris, waste as well as infectious and radioactive contaminated material from operation must be
disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning liquids as well
as section waste must be disposed of according to the respective regulations for special waste!

Thermo Scientific Cryostat Microm Cryo Star HM 560
Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Str. 49
69190 Walldorf / Germany
387 500 - English
11
Fig. 1

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
12
1 INTRODUCTION
1-1 DESCRIPTION OF THE
CRYO-STAR HM 560
Open top cryostat. Modular design. Stainless
steel cooling chamber. Temperature regulation
variable down to -50°C for specimen holder
and down to -35°C for knife carrier. Graphic
LC-display of preset and actual temperatures,
electronic control with user-oriented touchpad
keyboard, battery-buffered memory and self-
diagnostic system. Standby and sleep status
for noise reduction and energy savings.
Automatic evaporator defrosting and in addition
a manual defrost cycle which can be activated
when needed. Defrost interrupt on keystroke
request. Integrated rapid freezing device with
controlled cooling element down to -60°C.
Freezing device temperature independent of
cryo-chamber temperature. Detach function to
remove used specimen from specimen chucks.
Sliding window with heater and integrated,
position adjustable fluorescent lamp for cryo-
chamber. Storage space inside the cryo-
chamber. Flat storage space on top of the
housing.
HM-Rotary Microtome with backlash and
maintenance-free cross roller bearings in
stainless steel. Electro-motorized feed system.
Section thickness setting from 0,5 to 100
microns;
up to 2 µm in 0,5 µm-increments,
up to 10 µm in 1 µm-increments,
up to 20 µm in 2 µm-increments,
up to 50 µm in 5 µm-increments and
up to 100 µm in 10 µm-increments.
Trimming thickness setting from 5 to 500
microns;
up to 10 µm in 5 µm-increments,
up to 100 µm in 10 µm-increments,
up to 200 µm in 20 µm-increments and
up to 500 µm in 50 µm-increments.
Specimen retraction during the return stroke
with optical indication. Horizontal feed range 48
mm. Vertical specimen stroke 60 mm. Max.
specimen size 75 x 55 mm.
Motorized coarse feed in two directions with
three speed selections. Limit indication and
automated switch off at front and rear limits of
horizontal travel. Automatic approach system
for exact and safe approach of specimen
towards the knife edge.
Section counter and indication of sum of
section thicknesses with reset-button.
Indication of remaining travel. Hand wheel
brake in any position. Fine orientation of
specimen on two axes. Rotatable on Z-axis,
360°.
Basic outfit with three specimen chucks, cryo-
compound, 100 ml cryostat oil, brush shelf and
section waste tray.
A multi-use knife carrier is available. The
standard knife carrier is designed so the knives
as well as the disposable blade holder can be
easily clamped in place and adjusted.
Depending on the version, the disposable
blade holders SE take up low or high profile
blades. The corresponding anti-roll guides
facilitate taking off sections.

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
13
1-2 TECHNICAL DATA CRYO-STAR HM 560
Specimen temperature control:.......................................................................... +10°C to -50°C
Knife carrier control: ................................................................................ -10°C to -35°C
Fast freezing station: .................................................................................down to -60°C
Defrosting: automatic defrosting, adjustable with temperature control
manual immediate defrosting
Microtome: Section thickness range: ............................................. 1 - 500 µm
Fine section thickness range:.................................... 0,5 - 100 µm
Resolution: .................................................. 0,5 µm for 0,5 - 2 µm
..........................................................................1 µm for 2 - 10 µm
........................................................................2 µm for 10 - 20 µm
........................................................................5 µm for 20 - 50 µm
....................................................................10 µm for 50 - 100 µm
Trimming thickness range: ........................................... 5 - 500 µm
Resolution:........................................................5 µm for 5 - 10 µm
....................................................................10 µm for 10 - 100 µm
..................................................................20 µm for 100 - 200 µm
..................................................................50 µm for 200 - 500 µm
Knife carrier retraction during return travel...........................40 µm
Horizontal knife carrier range ............................................ 48 mm
Vertical specimen stroke ................................................... 60 mm
Read-outs: Graphical LC display: section thicknesses, section counter, sum of
section thicknesses, remaining travel to front end position, size of
the cutting window
Specimen approach: .......................................................................... automatic function
........................................alternatively manual with variable speed
Temperature parameter: ............................... temperature set values can be stored in pairs
Size of chucks: .......................................................................................30, 40 mm
............................................................. special sizes upon request
Specimen orientation: x - and y - axes: ......................................................... universal 8°
z - axis: ......................................................................... up to 360°
Coarse feed: ........................................... motorized, graduated and continuous
Cooling chamber illumination:................................................ with variable illumination position
Sliding window: .............................................................................................heated
Cutting speed: .................................................................................. 0 - 250 mm/s
Hand wheel brake: .................................................................................in any position
All temperatures refer to an ambient temperature of +20°C! Cont'd on page -2-

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
14
page -2-
1-2 TECHNICAL DATA CRYO-STAR HM 560
Storage
temperature range: ......................................................................... -20°C up to +50°C
Operating conditions: ....................... +5°C up to +35°C (at a max. rel. humidity of 60%)
...........................................................altitude up to 2000 m M.S.L.
.......................................................................... for indoor use only
Power requirements: ....................................................... 100 V 12 A +/-10% 50...60 Hz
...............................................................115 V 12 A +/-10% 60 Hz
.................................................220...230 V 6 A +/-10% 50...60 Hz
.................................................................240 V 6 A +/-10% 50 Hz
Pollution degree: ......................................................................................................2
Overvoltage category: ......................................................................................................II
Acoustic pressure: ......................................................................................... 45 dB(A)
...............................measured with 1 m distance to the instrument
Dimensions: ..Wide (w/o Hand wheel): 769 mm, deep: 932 mm, high: 578 mm
Weight: .............................................................................................141 kg
All temperatures refer to an ambient temperature of +20°C!

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
15
Fig. 2
1 = On indication (sleep status)
2 = Button specimen temperature
3 = Button knife temperature
4 = Button TRIM/FEED
5 = Button UP
6 = Button DOWN
7 = LC display
8 = Button fast freezing
9 = Button options
10 = Button detaching
11 = Scroll button
12 = Button RESET
13 = Button PROG
14 = Info line
15 = Status line

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
16
Fig. 3
1 = Button cutting window
2 = Button ACA
3 = Button coarse feed, forwards
4 = Button coarse feed, backwards
5 = Button TRIM

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
17
Fig. 4
1 = Symbol Hand wheel
2 = Button to activate the Hand wheel brake
3 = Button to loosen the Hand wheel brake
4 = Brake LED

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
18
2 OPERATING INSTRUCTIONS
2-1 SETTING UP THE CRYOSTAT
Note:
If the microtome cryostat is supplied
with an extra height-adjustable stage,
first unpack and set up this stage
according to the separate instruction manual.
Unpacking the instrument:
•Loosen and remove the four tightening
straps.
•Lift and remove the wooden top of the
packing.
•Remove the foam parts upwards.
•The four side parts of the packing, which are
connected with each other, can now be
removed upwards.
•Slightly tilt the cryostat to the side and
remove the right and left lower foam parts
one after the other.
•Lift the cryostat by the carrying handles (fig.
5.1 and 5.2) from the pallet.
•In case the handles are not installed, securely
install the attached carrying handles (fig. 5.1
and 5.2) at the front and rear side of the
instrument and carry the instrument to the
site of installation (table according to the
customer's choice) or place it onto the height-
adjustable stage.
Danger:
When placing the microtome cryostat
HM 560 onto the height adjustable
stage, it is absolutely necessary that
the stage is in its upper position.
Not observing this leads to the risk of
squeezing your hands between the rear
carrying handles and the stage housing.
•Remove the handles (fig. 5.1 and 5.2).
Caution:
Before the initial set-up of the height-
adjustable stage, it is absolutely
necessary to dismount the two rear
carrying handles (fig. 5.2) on the
cryostat, as when lowering the height-
adjustable stage, the handles will hit the stage
and cause damage to the stage.
Fig. 5

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
19
•Adjust the handles of the cryostat (fig. 5a.1
and 5a.2) on the rear side of the cryostat
stage.
•Carefully keep the shipping container for
possible return shipments.
Choose installation site that
•enough ventilation for the cooling system is
guaranteed.
•the distance between wall and rear panel is
approx. 10 - 15 cm.
•the suction areas on either side are kept free.
Moreover, the installation site must be free
from:
•draught by open doors or by air conditioning
systems.
•direct exposure to sunlight into the cooling
chamber.
Note:
Both measures reduce the formation of
frost and therefore result in more
favourable work conditions. A high air
moisture as well as high ambient temperatures
reduce the maximum performance of the
instrument.
In case the instrument is equipped with the
height-adjustable stage, turn the screws (fig.
5.5) on the front foot ends to fix the complete
unit.
Afterwards:
•Install the attached Hand wheel handle (fig.
5.3).
1
2
Fig. 5a

Thermo Scientific Cryostat Microm Cryo Star HM 560
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf
387 500 - English
20
2-2 INITIAL TURN-ON
As already mentioned in part 2-1, the height-
adjustable stage, if equipped, must be set up
according to the separate instruction manual
before the initial turn-on of the microtome
cryostat.
Caution:
Before turning on the instrument for the
first time, please check if the power
requirements indicated on the type
plate (fig. 7) correspond to the power supply
voltage being used.
•No other instruments should be connected to
the circuit used for the cryostat, as the
compressor needs high surge currents when
started.
•Do not use multi-socket power outlets and
extension lines for the supply of the stage.
•Connect the power line of the instrument to
the power outlet.
•Turn on the power switch (fig. 6.1) on the rear
side of the instrument.
•Close the heated sliding window (fig. 5.4) and
cool down the instrument.
Note:
To avoid frost built-up on the
specimen, the specimen temperature
is controlled to a temperature of +10°C
until the knife has reached a temperature
of -10°C.
The cooling phase will take approx. 1 to 2 h
depending on the set knife temperature
between -15°C and -30°C.
Fig. 6
1 = Power switch
2 = Mains plug
5 = Socket for option Vacutome/Disinfection
220...230V 6A 50...60Hz
Ser. No.XXXXX
Cat. No. 956320
Made in Germany
Type HM 560V
GmbH MICROM
69190 Walldorf, Germany
I VD
Cooling System
Refr.: R 404a
Amount: 0,275 kg
Max. Press.: 2300 kPa
Fig. 7
I
0
~___V
F1 / F2
2x __AT
Ma de in Germany
Fuse2x __ AT
GmbH MICROM
69190 Walldorf, Germany
Ser. No. ___ _
Cat. No. ______
_A / ___V +10%__ Hz
Type Stage f. HM 560 HV
GmbH
MICROM
69190 Walldorf, Germany
Cat. No. _ _____
Made in German y
Refr.: R 404a
Amount: 0,270kg
Max. Press.: 2300kPa
Refr.: R 134a
Amount: 0,180kg
Max. Press.: 2300kPa
LPCircuit
HPCircuit
Ser. No.____
Fuse 2 x __AT
__A / ___ V +10% __ Hz
Type HM560 MV
I
0
3
1
4
Fig. 8