Thermo Scientific Orion ROSS Ultra User manual

ROSS Ultra, ROSS Ultra Triode
and ROSS pH Electrodes
263745-001 •Revision B •November 2014
Thermo Scientic Orion
User Manual

Contents
Introduction 4
ROSS Electrode Families 4
ROSS Ultra Electrodes 4
ROSS Ultra pH/ATC Triode Electrodes 4
ROSS Electrodes 5
ROSS Sure-Flow Electrodes 5
ROSS Semi-micro Electrodes 5
ROSS micro pH Electrodes 5
Required Equipment 5
Electrode Preparation 6
Preparation for Refillable ROSS Electrodes 6
Preparation for ROSS Sure-Flow Electrodes 7
Preparation for Gel-Filled ROSS Ultra Triode Electrodes 8
Sample Requirements 8
Measuring Hints 9
Electrode Calibration 10
General Calibration Procedure 10
One Buffer Calibration 10
Two Buffer Calibration 11
pH Measurements 12
Electrode Storage 12
Electrode Maintenance 12
Electrode Cleaning Procedures 12
General Cleaning 12
Cleaning Solutions 13
Electrode Characteristics 13
Temperature Effects 13
Sure-Flow Electrode Leak Rate 14
ROSS Micro pH Electrode 14
ROSS Ultra pH/ATC Triode Electrodes 15
ROSS Electrode Operation with Orion Versa star pH/
LogR Meters 15
Electrode Interferences 16
Troubleshooting 17
pH Meter 17
Electrode 17
Sample/Application 17
Technique 17
Assistance 18
Warranty 18
RoHS Statement 18
Ordering Information 19
Specifications 21

4|ROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes Thermo Scientic Orion
Introduction
This user manual contains information on the preparation, operation
and maintenance of the Thermo Scientific™Orion™ROSS Ultra™,
Thermo Scientific™Orion™ ROSS™, and Thermo Scientific™Orion™
ROSS™Triode™electrodes.
All ROSS pH electrodes have a pH range of 0 to 14, pH precision of
0.01 and temperature range of 0 to 100°C. ROSS electrodes can be
used in samples that contain TRIS, sulfides and proteins since they do
not contain silver or mercury.
ROSS electrodes incorporate the distinctive ROSS internal reference
system that provides superior measurement stability, faster response,
greater accuracy and more reproducible results than conventional
electrodes. ROSS electrodes have a double junction reference, so
the fill solution can be modified to more closely match the sample
composition in high pH, low pH or non-aqueous solutions.
ROSS electrodes provide readings stable to 0.01 pH in less
than 30 seconds, even in the extreme case of samples varying from
one another by 50°C or more. Results are three to five times more
precise than those obtained with conventional electrodes. Over the
lifetime of the electrode, ROSS reference potential drifts less than an
average 0.12mV per day, which corresponds to 0.002 pH units, so
recalibration is minimized.
• The majority of ROSS electrodes with a BNC waterproof or BNC
connector have a one meter (three foot) long cable, except where
noted.
• All electrodes with a screw cap connection require a separate cable.
• Electrodes with a waterproof BNC connector can be used on any pH
meter with a BNC connection.
• The 800500U ROSS Ultra reference half-cell and 800300 ROSS
reference half-cell can be used with the 8101BNWP and 8101SC
ROSS pH half-cell electrodes or 8411BN ROSS sodium half-cell
electrode.
ROSS Electrode Families
ROSS Ultra Electrodes
ROSS Ultra electrodes offer the ultimate measurement stability and the
least long-term drift, plus a two year warranty for refillable models and
an 18 month warranty for gel-filled models.
ROSS Ultra pH/ATC Triode Electrodes
ROSS Ultra pH/ATC Triodes have a built-in temperature probe,
so pH and temperature are measured with one electrode.
Gel-filled ROSS Ultra pH/ATC Triode electrodes have an 18 month
warranty and refillable ROSS Ultra pH/ATC Triode electrodes have a 24
month warranty.

Thermo Scientic ROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes |5 Thermo Scientic Orion
ROSS Electrodes
ROSS electrodes provide precise pH measurements and measurement
stability regardless of temperature changes or sample composition.
ROSS Sure-Flow Electrodes
ROSS Sure-Flow electrodes incorporate a free-flowing junction that is
easy to clean and never clogs. These electrodes are ideal for dirty or
viscous samples that clog traditional electrodes.
ROSS Semi-micro Electrodes
ROSS semi-micro electrodes are designed for applications where
sample volume is limited and can take measurements in samples of 0.2
ml volume. They are available with chemically-resistant glass (6 mm
tip) or epoxy (8 mm tip) bodies, Sure-Flow junction and ROSS Ultra
long-life models.
ROSS micro pH Electrodes
ROSS micro pH electrodes have been designed to measure
extremely small samples. The measuring tip is 3 mm in diameter and
40 mm long. The minimum depth of immersion is 4.5 mm and the
minimum sample size is 15 μL in 384 well plates.
Required Equipment
1. Thermo Scientific™Orion™Versa star™, Thermo Scientific™Orion
Star™A300 series, Thermo Scientific™Orion Star™A200 series,
Thermo Scientific™Orion Star™A100 series or Thermo Scientific™
Orion™ Dual star™meter with pH measurement capability,
such as the Versa star benchtop meter with pH module, Star
A211 pH benchtop meter or Star A321 pH portable meter;
except 8107BNURCA, 8157BNURCA and 8302BNURCA Triode
electrodes which are designed for use with a meters which support
a thermister with a 30K NTC curve and have an RCA plug, such as
the Five and Seven Series METTLER TOLEDO™pH meters.
ROSS electrodes can be used on any pH meter with a BNC
connection. The electrodes can also be used on meters with
a variety of inputs when an adapter cable is used. Visit www.
thermoscientific.com/water for details.
2. Orion ROSS Ultra, and ROSS Triode electrodes.
• The 8302BNUMD, 8107BNUMD, 8107UWMMD, 8157BNUMD
and 8157UWMMD pH/ATC Triode electrodes have a
temperature connector that is compatible with Orion Star A
series, Versa star and Dual star pH meters.
• The 8107BNURCA, 8157BNURCA and 8302BNURCA pH/
ATC Triode electrodes have a temperature connector that is
compatible with meters which support a thermister with a 30K
NTC curve and have an RCA plug, such as the Five and Seven
Series METTLER TOLEDO pH meters.
3. ROSS fill solution, Cat. No. 810007.
Note: Do not use any fill solution that contains silver, as silver
will damage ROSS electrodes.