Thermo Scientific Orion 320 User manual

Orion PerpHect Meter
INSTRUCTION MANUAL
yes
s
res print
mV
ready
pH
°C
setup
t
modecal
Orion 330
basic PerpHecT LogR pH
Orion 310,
320,
330
Analyze •Detect •Measure •Control™

AQUAfast, Cahn, EZ Flash, Ionalyzer, ionplus, KNIpHE, No Cal, ORION, perpHect, PerpHecT, PerpHecTion, pHISA, pHix, pHuture, Pure Water, Sage, Sensing the Future,
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trademarks of Thermo.
Guaranteed Success and The Technical Edge are service marks of Thermo.
PerpHecT meters are protected by U.S. patent 6,168,707.
PerpHecT ROSS are protected by U.S. patent 6,168,707.
ORION Series A meters and 900A printer are protected by U.S. patents 5,108,578, 5,198,093, and German patents D334,208 and D346,753.
Sure-Flow electrodes are protected by European Patent 278,979 and Canadian Patent 1,286,720.
ionplus electrodes and Optimum Results solutions are protected by US Patent 5,830,338.
ROSS Ultra electrodes have patents pending.
ORION ORP Standard is protected by US Patent 6,350,367.
ORION Series A conductivity meters are protected by US Patent 5,872,454.
© Copyright 2003, Thermo Electron Corporation. All rights reserved. Question everything, and Analyze. Detect. Measure. Control are trademarks of Thermo Electron
Corporation.
The specifications, descriptions, drawings, ordering information and part numbers within this document are subject to change without notice.
This publication supersedes all previous publications on this subject.

Table of Contents
Chapter I General Information ................................................................ 1
Introduction ..................................................................................... 1
Chapter II Meter Description ................................................................... 3
A. Display ....................................................................................... 3
B. Keypad ....................................................................................... 5
Chapter III Orion 310 Meter Set-Up ........................................................... 7
A. Power Source ............................................................................. 7
B. Electrode Connection ................................................................. 7
Chapter IV Using the Orion 310 ................................................................ 9
A. Power-Up ................................................................................... 9
B. Meter Self-Test Procedure ......................................................... 9
Chapter V General Information on pH Calibration for Orion 310........ 11
A. Autocalibration with Two Buffers ........................................... 12
B. Autocalibration with One Buffer ............................................. 13
Chapter VI Introduction to Patented digital
LogR pH Measurement for Orion 310 .................................. 15
A. What is digital LogR? .............................................................. 16
B. Orion 310 Temperature Calibration Procedure ....................... 17
Chapter VII Temperature Measurement for Orion 310 ........................... 19
A. Temperature Compensation (Manual and Automatic) ............ 19
Chapter VIII Orion 320 Meter Set-Up ......................................................... 21
A. Power Source ........................................................................... 21
B. Electrode Connection ............................................................... 21
Chapter IX Using the Orion 320 .............................................................. 23
A. Power-Up ................................................................................. 23
B. Meter Self-Test Procedure ....................................................... 23
C. SETUP Mode for Orion 320 .................................................... 25
Chapter X General Information on pH Calibration for Orion 320........ 27
A. Autocalibration with Two Buffers ........................................... 28
B. Autocalibration with One Buffer ............................................. 29
C. Manual pH Calibration............................................................. 30
Chapter XI Introduction to Patented digital
LogR pH Measurement for Orion 320 .................................. 31
A. What is digital LogR? .............................................................. 32
B. Orion 320 Temperature Calibration Procedure ....................... 33
PerpHecT Orion 310, 320, 330 Instruction Manual

Chapter XII Orion 320 Other Measurement Modes................................. 35
A. mV/ORP Measurement Orion 320........................................... 35
B. Temperature Compensation ..................................................... 35
Chapter XIII Orion 330 Meter Set-Up ......................................................... 37
A. Power Source ........................................................................... 37
B. Electrode Connection ............................................................... 37
Chapter XIV Using the Orion 330 .............................................................. 39
A. Power-Up ................................................................................... 3
B. Meter Self-Test Procedure ....................................................... 39
C. SETUP Mode Orion 330 .......................................................... 40
Chapter XV General Information on pH Calibration for Orion 330........ 43
A. Autocalibration with Two Buffers ........................................... 44
B. Autocalibration with One Buffer ............................................ 45
C. Manual pH Calibration............................................................. 46
Chapter XVI Introduction to Patented digital
LogR pH Measurement for Orion 330 .................................. 47
A. What is digital LogR? .............................................................. 48
B. Orion 330 Temperature Calibration Procedure ....................... 49
Chapter XVII Orion 330 Other Measurement Modes................................. 51
A. mV/ORP Measurement Orion 330........................................... 51
B. Temperature Compensation ..................................................... 51
Chapter XVIII Self-Test Definitions Orion 310, 320 and 330 ..................... 53
Chapter XIX Operator Assistance Codes Orion 310, 320 and 330 ......... 55
Chapter XX Troubleshooting Guide ......................................................... 59
Chapter XXI Repair and Service ................................................................ 61
Chapter XXII Warranty ................................................................................. 63
Chapter XXIII Notice of Compliance ............................................................ 67
Chapter XXIV Ordering Information ............................................................ 69
Chapter XXV Specifications For PerpHecT Meters ................................... 73
Appendix A LogR Temperature Accuracy Tables ................................... 75
PerpHecT Orion 310, 320, 330 Instruction Manual

1
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
Chapter I
General Information
Introduction
Orion provides pH and ISE meters for every application from basic pH
measurements to advanced Ion Selective Electrode work. This manual
describes the operation and use of the Orion PerpHecT™meter line. The
PerpHecT meters consist of the following meters: the Orion 310 pH/
Temperature Meter, the Orion 320 pH/mV/ORP/Temperature Meter, and
the Orion 330 pH/mV/ORP/Temperature Meter.
All PerpHecT meters feature the Orion patented digital LogR™technology
which allows fully temperature compensated measurements without the
need for a separate ATC probe.
Figure 1 — PerpHecT Orion 330
General Information
mV
pH
˚c
set up
4
7
10
man
ready
ATC
print
mode
setup
yes res
cal
basic PerpHecT LogR pH
Orion 330
LogR

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
2General Information
All meters are microprocessor-controlled and feature pH autocalibration,
patented digital LogR pH measurement mode, sealed keypads and large
easy-to-read LED displays.
These systems are ideal for busy, multi-user laboratories or plant
environments. pH measurements are easy with the help of advanced
features such as autocalibration, LogR mode, automatic temperature
compensation, choice of pH resolution and diagnostic operator
assistance codes.
The Orion 310 is a basic pH Meter which comes complete with a
PerpHecT combination glass pH temperature electrode. The choice of
a PerpHecT Ag/AgCl electrode (Orion 9202BN) or PerpHecT ROSS™
electrode (Orion 8202BN) is available.
The Orion 320 is a pH/mV/ORP/Temperature Meter which comes
complete with a PerpHecT combination glass electrode. The choice of
a PerpHecT Ag/AgCl electrode (Orion 9202BN) or PerpHecT ROSS
electrode (Orion 8202BN) is available.
The Orion 330 is a pH/mV/ORP/Temperature Meter with RS232
interface for connections to a printer or a computer. It comes complete
with a PerpHecT combination glass electrode. The choice of a PerpHecT
Ag/AgCl electrode (Orion 9202BN) or PerpHecT ROSS electrode (Orion
8202BN) is available.
Using this Manual
Operating instructions for the Orion 310 are covered in Chapters III
through VII.
Operating instructions for the Orion 320 are covered in Chapters VIII
through XII.
Operating instructions for the Orion 330 are covered in Chapters XIII
through XVII.

3
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
Figure 2 — PerpHecT Orion 330 Display
Chapter II
Meter Description
A. Display
Orion 310
A large bright LED display shows pH results and temperature in the main
field. The current operation mode is indicated along the right side of the
display. “Ready” is designated by the indicator along the left side of the
display. See Figure 2.
Orion 320 and 330
A large bright LED display shows results in the main field. Temperature
is displayed in the upper field. The current operation mode is indicated
along the right side of the display. The buffers used during the last pH
calibration are designated by the indicators along the left side of the
display. See Figure 2.
Meter Description
mV
pH
˚c
set up
4
7
10
man
ready
ATC
LogR

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
4Meter Description
SET UP (Orion 320 and 330)
Indicates that the meter is in the setup mode which is used to define or
view operating parameters.
LogR
Indicates that the meter is in the LogR temperature measurement or
calibration mode. LogR is activated in the setup mode (or with the temp
key Orion 310) and measured with the pH electrode. LogR function is
activated when the LogR icon appears in the upper display.
ATC
Indicates that an ATC probe is connected and operational. Manual
Temperature Compensation is activated when the ATC and LogR symbol
are not displayed.
READY
Displayed when the electrode signal has stabilized. Also displayed
when calibration standard input (pH and Temp) has stabilized.
° C
The ATC icon in the upper display is lit when temperature is measured
with an ATC probe. The LogR icon is lit when LogR temperature
measurement is activated. If neither is on, manual temperature setting
is active. For the Orion 310, temperature is displayed by pressing
MODE key.

5
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
B. Keypad
Orion 310
The PerpHecT Orion 310 meter has a 6 button keypad used to control all
meter functions. Each keypress is acknowledged by the meter via a
positive feel response.
Key Function
mode Press to select MEASURE mode. Use to toggle display
between Temperature and pH.
yes Press to accept settings or calibration points.
scroll (▲,▼)Press to change a parameter before it is entered. Also used
to scroll Manual Temp settings in the Temperature mode.
cal Press button to initiate pH or LogR temperature calibration.
temp Press to enable or disable LogR Function.
Orion 320
The PerpHecT Orion 320 meter has a 6 button keypad used to control all
meter functions. Each keypress is acknowledged by the meter via a
positive feel response.
Key Function
mode Press to select MEASURE mode. Used to initiate LogR
temperature calibration when LogR function is enabled.
yes/res Press to accept settings, calibration points or to change
display resolution.
scroll (▲,▼)Press to change a parameter before it is entered. Also used,
in MEASURE mode, to scroll Manual Temp settings.
cal Press button to initiate pH calibration.
setup Press to enter Setup mode. Setup is entered to change
operating parameters and to enter manual calibration buffer
values.
Meter Description

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
6Meter Description
Orion 330
The PerpHecT Orion 330 meter has an 8 button keypad used to control all
meter functions. Each keypress is acknowledged by the meter via a
positive feel response.
Key Function
mode Press to select MEASURE mode. Used to initiate LogR
temperature calibration when LogR function is enabled.
yes Press to accept settings or calibration points. May also be
used to scroll through the setup menu without changing
any parameters.
scroll (▲,▼) Used to initiate a change in current setup parameters.
Press to change a parameter before it is entered. Also used
to scroll Manual Temp settings in MEASURE mode.
print Press to print measurement data.
cal Press button to initiate pH calibration.
setup Press to enter Setup mode. Setup is entered to change
operating parameters and to enter manual calibration
buffer values.
res Press to change display resolution.
Figure 3 — Orion PerpHecT Meter Keypad
print
mode
setup
yes res
cal
basic PerpHecT LogR pH
Orion 330

7
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
BNC
Input Recorder
ATC
RS232 Line Cord
Input
Reference
Input
Figure 4 — PerpHecT Orion 330 Connections
Chapter III
Orion 310 Meter SetUp
A. Power Source
All PerpHecT meters are designed to operate with a line adaptor
(Orion 020125) for 110V operation or (Orion 020130) for 220V operation.
Plug the line adaptor securely into the meter’s Line Adapter Input, then
plug into an appropriate wall outlet.
B. Electrode Connection
1. Electrode
Attach the PerpHecT pH electrode or other pH electrode with BNC
connector to the BNC input on the meter by sliding the connector
onto the BNC input, then push in and turn clockwise to lock into position.
Electrodes with U.S. Standard connectors may be used with a an adaptor
(Orion 090033). Connect reference electrodes with pin tip connectors by
pushing connector directly into reference input.
See Figure 4.
2. ATC Probe
Attach ATC probe to the ATC input by sliding the connector straight in
until firmly in place. Use Orion ATC probes Orion 927006
or 927005 only.
Orion 310 Meter SetUp

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
8

9
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
Chapter IV
Using the Orion 310
A. Power-Up
Plug meter into an approved wall outlet via the power line adaptor. The
entire display (all annunciators) will be displayed for 2 seconds. Once all
power-up procedures have been completed, the meter automatically
advances to measure mode.
NOTE: If this is the first time that the meter has
been powered up, it is recommended that the Self-
Test Procedure be performed before using the meter
for measurements.
B. Meter Self-Test Procedure
It is recommended that this procedure be performed when the meter is
operated for the first time or any time that operational problems arise.
For a description of the diagnostic tests, see Chapter XVIII Self-Test
Definitions pg 53.
This procedure verifies the proper operation of the PerpHecT meters.
Self-test:
1. Attach shorting cap to electrode BNC input.
2. Plug line adapter (Orion 020125) securely into meter Line Adapter
Input then into an appropriate wall outlet while pressing the YES
key (hold down for 3 seconds) to start the self-test. The meter will
automatically perform seven diagnostic tests.
3. When “0” appears in the display field, press each key once.
A numeric digit will be displayed upon each keypress.
4. After the self-test is completed, the meter will automatically go to
MEASURE mode.
5. If any problems are found during the self-test see Operator
Assistance Codes, page 55.
Using the Orion 310

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
10 Using the Orion 310
Check Out:
After completing the self-test the meter will be in MEASURE mode.
1. Press the MODE key until the pH mode indicator is
displayed. Main display should read a steady 7.00 ±0.02.
2. If not, press CAL key. Select a one-point calibration “7” to
change the pH reading. When READY lights up, press YES.
3. The main display should read 100.0 after the legend SLP is
displayed. If so, press YES. If not, use the SCROLL (▲,▼)
keys to change the value to 100.0 press YES.
4. The meter advances to pH mode and the display should now
read a steady 7.00 ±0.02.

11
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
Chapter V
General Information on
pH Calibration for Orion 310
A one or two-point calibration should be performed using fresh buffers
before pH is measured. It is recommended that a two buffer calibration,
using buffers that bracket the expected sample range, be performed at the
beginning of each day to determine the slope of the electrode. This serves
the dual purpose of determining if the electrode is working properly, and
storing the slope value in memory. For maximum accuracy, perform a
one-buffer calibration every two hours to compensate for electrode drift,
using a fresh aliquot of pH 7 buffer.
When operating in the LogR mode, the meter does not require the use of a
separate ATC probe. The PerpHecT meters measure the resistance of the
pH sensing glass and converts the value to temperature-compensated pH
measurements. For optimum results, PerpHecT electrodes or PerpHecT
ROSS electrodes should be used.
For best results, it is recommended that the unit be calibrated for
temperature in the LogR mode. Alternately, an ATC probe may be used.
If LogR or an ATC probe is not used, all samples and standards should
be at the same temperature or manual temperature compensation should
be used.
Stir all buffers and samples with a magnetic stirrer while a measurement is
being made.
NOTE: Some magnetic stirrers generate enough
heat to change solution temperature. To avoid this,
place a piece of cardboard, foam rubber, or other
insulating material between the stir plate and
beaker.
Always use a fresh aliquot of buffer whenever calibrating.
General Information on pH Calibration for Orion 310

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
12 General Information on pH Calibration for Orion 310
A. Autocalibration with Two Buffers
1. Attach the pH electrode to the meter.
2. Choose either pH 4.01 and pH 7.00, or pH 7.00 and pH 10.01
buffers; whichever will bracket your expected sample range.
3. Press CAL key to initiate calibration sequence. CAL is displayed
for two seconds. Press the YES key to accept the last calibration
range (7-4 or 7-10) or select one of these calibration ranges using
the SCROLL (▲,▼) keys. Press the YES key to accept. pH 7 will
be displayed for two seconds. Place electrode in pH 7.00 buffer.
Reading will be displayed and updated as calibration continues.
When the READY light comes on, indicating electrode stability,
press the YES key to accept.
4. pH 4 (or pH 10) will then be displayed for two seconds. Remove
electrode from pH 7.00 buffer. Rinse with deionized water and
place electrode in either pH 4.01 or 10.01 buffer (depending on
calibration range that was selected). When the READY light
comes on, press the YES key to accept buffer value. SLP will
be displayed for two seconds, then the calculated slope will be
displayed.
5. Meter will automatically go into the MEASURE mode. The
READY light will come on to indicate electrode stability.
6. If operating in the LogR mode or using an ATC probe, the
temperature-corrected pH reading is displayed.
7. Rinse electrodes with deionized water and place into sample.
Record pH directly from the main meter display when the READY
light is displayed or when electrode signal is stable.
NOTE: When making measurements in LogR
mode, you must calibrate for temperature before
attempting a pH calibration. See page 17 for
temperature calibration procedure.

13
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
General Information on pH Calibration for Orion 310
B. Autocalibration with One-buffer
NOTE: Autocalibration with one-buffer can only be
performed using pH buffer 7.00.
1. Attach the pH electrode to the meter.
2. Choose pH 7.00 Buffer.
3. Press CAL key to initiate calibration sequence. CAL is displayed
for 2 seconds. Select calibration range “7” using the SCROLL
(▲,▼) keys. Press the YES key to accept. pH 7 will be displayed
for two seconds. Place electrode in pH 7.00 buffer. Reading will be
displayed and updated as calibration continues. When the READY
light comes on, indicating electrode stability, press the YES key
to accept.
4. SLP will be displayed for two seconds. Then 100.0 (or previous
calibration slope value) will be displayed. This value can be edited
using the SCROLL (▲,▼) keys. Press the YES key to enter
the value.
5. Meter will automatically go into the MEASURE mode.
The READY light will come on to indicate electrode stability.
6. If operating in the LogR mode or using an ATC probe, the
temperature-corrected pH reading is displayed.
7. Rinse electrodes with deionized water and place into sample.
Record pH directly from the main meter display when the READY
light is displayed or when electrode signal is stable.

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
14

15
PerpHecT
®
Orion 310, 320, 330 Instruction Manual
Chapter VI
Introduction to Patented digital
LogR pH Measurement for
Orion 310
Obtaining accurate pH values depends on both correct measurement of
standard and sample temperatures and on compensation for any variations
in these temperatures. This thermal compensation has generally been
accomplished either manually (by measuring sample solution with a
thermometer and then dialing in the measured temperature on the meter),
or automatically (by placing a separate temperature probe together with
a pH electrode in the solution).
The temperature of a solution affects pH measurements in two principal
ways:
1. The pH of buffers and samples actually changes slightly with
temperature. For buffers, this change is known and is built into
the PerpHecT series of meters.
2. The slope of the calibration, which the meter uses to convert
millivolt readings to pH, changes with temperature. At 25°C,
a 10 degree change in temperature changes the slope by 3.4%.
How important is direct temperature compensation to pH measurement?
To see the effects of temperature, look up your approximate sample
temperature on the left side of Table 1. Read across the top to your
approximate pH value. The value shown at the intersection is the error
a 1°C change in temperature will cause in pH measurement without
compensation. Accurate temperature compensation is essential to accurate
pH measurement.
Introduction to Patented digital LogR pH Measurement for Orion 310

PerpHecT
®
Orion 310, 320, 330 Instruction Manual
16
Table 1 pH Error due to 1°C Temperature Change (in pH units)
A. What Is digital LogR?
PerpHecT pH meters allow for direct temperature measurement and
temperature compensation from your pH electrode. With simultaneous
measurement of pH and temperature, the patented Orion digital LogR
technology makes it possible using most standard glass pH electrodes.
The system is based on using the electrical resistance of the glass sensing
bulb as the temperature probe. The name comes from the fact that the
logarithm of the resistance of the bulb varies almost linearly with the
reciprocal of the absolute temperature (See Figure 5). Almost all common
pH glasses show a similar decrease in resistance with increasing
temperature, and can be used with other types of meters. However,
only the exact shape of the curve for Orion PerpHecT electrodes
is in the meter’s memory.
To maximize system performance and accuracy, use PerpHecT ROSS and
PerpHecT pH electrodes.
Introduction to Patented digital LogR pH Measurement for Orion 310
10
20
30
40
50
60
70
80
90
0.004
0.003
0.003
0.003
0.003
0.003
0.003
0.003
0.003
0.011
0.010
0.010
0.010
0.009
0.009
0.009
0.008
0.008
0.007
0.007
0.007
0.006
0.006
0.006
0.006
0.006
0.006
0.014
0.014
0.014
0.013
0.012
0.012
0.012
0.011
0.011
0.018
0.017
0.017
0.016
0.015
0.015
0.015
0.014
0.014
pH 12
or pH 2
pH 11
or pH 3
pH 10
or pH 4
pH 9
or pH 5
pH 8
or pH 6TEMP. (°C)
2.7
-1 01234567
2.6
2.8
2.9
3
3.1
3.2
3.3
3.4
3.5
3.6
3.7
3.8
Temperature (1000/Degrees Kelvin)
Figure5
Log of Resistance
Cal Point Values
Calculated Values