ThermoFisher Scientific GeneChip 645i User manual

For Research Use Only. Not for use in diagnostic procedures.
GeneChip™ Hybridization Oven 645i
USER GUIDE
Catalog Numbers 00-0331
Publication Number MAN0027670
Revision F

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The information in this guide is subject to change without notice.
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IT.
Revision history: Pub No. MAN0027670
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©2007, 2008, 2010, 2020, 2023 Thermo Fisher Scientific Inc. All rights reserved.
Affymetrix Inc.
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Products: GeneChip™ Hybridization Oven 645i
Revision Date Description
F May 2023 Initial release in Thermo Fisher Scientific document control system. Supersedes legacy Affymetrix publication number
08-0255. Changed all 645 references to 645i. Updated all instrument images. Added this revision history table.
E October 2010
Updated specification template. Updated to the current document template, with associated updates to trademarks, logos,
licensing, and warranty. Updated compliance information. Added the statement "The power cord is the disconnect device.
Do not position the equipment so that it is difficult to operate the disconnecting device."
D January 2010 Updated User Guide to new template.
C February 2008 Updated User Guide with new print specifications.
B October 2007 Updated to include GeneChip Hyb Oven 645 User Guide PRINTER'S FILE.pdf.
A July 2007 Initial release.

GeneChip™ Hybridization Oven 645i User Guide 3
Contents
CHAPTER 1 The Hybridization Oven 645i . . . . . . . . . . . . . . . . . . . . . . . 4
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Quick reference guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Unpacking and setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Unpacking the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Operating the door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Using the rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Installing the rotisserie into the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Operating the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
The front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Loading the rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Unloading the rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Communicating with the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Serial communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Oven maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Replacing the fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
GeneChip™ Hybridization Oven 645i specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Physical dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Power requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Environmental requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Communications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Regulatory requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
China RoHS Restriction of Hazardous Substances compliance . . . . . . . . . . . . . . . . . . . . . . 26
China RoHS Restriction of Hazardous Substances compliance . . . . . . . . . . . . . . . . . . . . . . 27
Customer and technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Limited product warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4GeneChip™ Hybridization Oven 645i User Guide
1The Hybridization Oven 645i
Introduction
The Applied Biosystems GeneChip™ Hybridization Oven 645i (Figure 1) is a general
purpose laboratory oven with a rotating rotisserie designed for hybridization of
Applied Biosystems GeneChip™ cartridge arrays. Electronic controls accurately
govern the oven temperature and provide visible status information to the operator.
Key features of the oven include:
• Programmable temperature range from 30.0°C to 70.0°C
• Programmable rotation rate range from 10 rpm to 80 rpm
• Keypad programming
• Excellent temperature accuracy and uniformity
• Ergonomic design for ease of use
• Holds up to 64 GeneChip™ cartridge arrays
• Pause / Jog control
• Highly visible status indicators
• Status information available via RS232 serial port
• Stackable (2 units high)
• Error detection and shutdown in case of malfunction
The oven has a glass door for observing samples during processing. Interior surfaces
are of stainless steel for high durability and low maintenance.
Figure 1 The GeneChip™ Hybridization Oven 645i.

GeneChip™ Hybridization Oven 645i User Guide 5
Chapter 1 The Hybridization Oven 645i
Quick reference guide 1
Quick reference guide
The hybridization oven is very simple to operate (Table 1).
Table 1 Quick reference guide for the Hybridization Oven 645i
If you want to: Then do this: Description
Turn on/off power. 1. Press the front, left I/O button.
2. Press PAUSE to view the current
settings and operate the oven.
Controls line power to the unit.
Adjust rotisserie motor
speed, pause the motor
or jog the motor.
1. Press the UP or DOWN button until
the set point blinks to access the
set point.
2. Press the UP or DOWN button to
set the speed (rpm).
3. Press PAUSE to pause the
rotisserie.
4. Press PAUSE again to take the unit
out of pause to program and run
the oven.
5. When the door is open, you can
use the PAUSE button as the JOG
button.
These controls adjust the speed of the rotisserie
motor.
Adjust the oven
temperature.
1. Press the UP or DOWN button until
the set point blinks to access the
set point.
2. Press the UP or DOWN button to
set the temperature (centigrade).
These controls are associated with the heating
function.

Chapter 1 The Hybridization Oven 645i
Safety information
6GeneChip™ Hybridization Oven 645i User Guide
1
Safety information
These instructions contain important operating and safety information. You must
carefully read and understand these instructions before using the hybridization oven.
Applied Biosystems has designed the hybridization oven to optimize function,
reliability, safety, and ease of use. It is your responsibility to install and use the
hybridization oven properly.
Follow these safety instructions regarding the hybridization oven:
• This equipment is for indoor use only.
• Connect the unit to a properly grounded electrical outlet of the correct voltage
and current handling capacity. Check the nameplate on the back of the unit for the
voltage and current rating.
• Do not remove or modify the grounded power plug. Use only properly grounded
outlets to avoid a shock hazard.
• If replacing fuses, note that the oven employs double pole fusing. The power
entry module, located on the rear panel of the unit, contains two fuses, one for
each side of the AC power circuit. Replace these only with 5x20 mm F6.3A (fast
acting) 250V rated fuses, as marked on the rear panel of the oven. (See "Replacing
the fuses" on page 22.)
• Disconnect the unit from the power outlet before performing any cleaning,
maintenance, or service.
• The power cord is the disconnect device. Do not position the equipment so that it
is difficult to operate the disconnecting device.
• Always verify that the rotisserie has stopped turning before reaching into the
oven.
• The interior of the oven and its contents can reach temperatures that can cause
burns. Avoid immediate contact with the oven interior upon opening the door, or
wear protective gloves. The interior of the unit will remain hot without visual
indication for some time after the door is open or power is switched off.
• If you plan to use the unit with any procedure that will give off fumes, be sure to
operate the unit in a fume hood or with proper ventilation.
• The hybridization oven is not intended for use with flammable materials. If used
with flammable materials, fire or explosion hazard may result.
• If radioactive materials are used in processing, user must comply with all
regulations regarding the acquisition, use, and disposal of such materials.
CAUTION! If you use this instrument in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired and
the warranty may be voided.
WARNING! The oven weight is approximately 67 pounds (30 kg). To avoid
injury, use 2 persons when lifting the oven.

GeneChip™ Hybridization Oven 645i User Guide 7
Chapter 1 The Hybridization Oven 645i
Unpacking and setup 1
• Do not continue to use the oven if the temperature or rotisserie controllers fail, as
evidenced by errors E01 through E12 being displayed (see the section, "Error
codes and troubleshooting" on page 21). Contact Thermo Fisher Scientific
Technical Support.
• Use appropriate hand and eye protection when handling hazardous chemicals.
• Replace the fuses with same type (see the section, "Replacing the fuses" on page
22).
NOTE: Verify that 115/230V switch is set correctly:
• 115 for Americas and Japan
• 230 for EU and most of the world
Standard IEC 320 power entry with fuse compartment on rear panel of oven
NEMA 5-15P (US standard) cord supplied with unit.
Local power cord must be supplied for EU, rest of world
t
Unpacking and setup
Each hybridization oven is shipped in a single carton. When unpacking the unit, check
each item against the packing list below. If something is missing, contact Thermo
Fisher Scientific Technical Support.
The GeneChip™ Hybridization Oven 645i accessories include:
• Rotisserie
• Eight plastic GeneChip™ cartridge carriers (Two are included in each box.)
– Four red (Part No. 90-0356)
– Four white (Part No. 90-0359)
• Eight spring steel carrier retaining clips (Part No. 10-0458)
• RS232 communications cable, 5 meters long, for serial port—workstation
communication
• Oven power cord (US NEMA 5-15P style standard)
CAUTION! DOUBLE POLE NEUTRAL FUSING. The power entry module
located on the rear of the unit contains two fuses, one located in each side of
the AC line.
IMPORTANT! In case of power interruption, if the interruption is no longer than
10 seconds, the oven will restart automatically at the originally programmed
temperature set point and rotation rate. If the interruption lasts longer than 10 seconds,
the oven will restart in PAUSE mode, in which both heating and rotation are
suppressed. You must press the PAUSE button to reactivate the oven. If you experience
frequent power interruptions in your locality, consider purchasing an uninterruptible
power supply (UPS).
WARNING! The oven is heavy. Two people are required to lift the oven.

Chapter 1 The Hybridization Oven 645i
Unpacking and setup
8GeneChip™ Hybridization Oven 645i User Guide
1
Locating the oven • Use a strong, level table or laboratory bench capable of supporting the weight of
the oven. The oven weighs approximately 67 pounds (30 kg).
• Provide an adequate source of electrical power within reach of the power cord.
The oven operates on AC voltages from 100 to 240 volts nominal, 50/60 Hz, and
draws currents below 5 Amperes.
• Provide minimum clearance as follows: 0.5 inch (13 mm) from top, 2 inches (50
mm) from the back and each side, and 18 inches (460 mm) for door swing at the
front of the unit. Note that there is an air intake fan located in the rear panel. Do
not block this vent.
• Provide adequate bench clearance for cartridge preparation, loading and
unloading cartridge carriers.
• Avoid extreme ambient temperatures and locations close to heaters or air
conditioning vents.
• Keep the oven dry—avoid locations close to sinks or bulk liquid dispensers and
storage.
Unpacking the
oven
NOTE: The oven is packaged in a corrugated shipping crate that can be reuse if you
need to return the oven for service. However, if the crate has suffered significant
crushing, cutting or structural damage, and you need to return the oven to Thermo
Fisher Scientific, we recommend that you discard the crate and use new shipping
materials.
1. Remove the oven from its corrugated box and styrofoam protectors.
2. Place it on a sturdy table or laboratory bench.
3. Remove protective wrappings.
4. Pull on the handle to open the oven door and remove the box of accessories.
5. Verify that the oven chamber is free of paper, cardboard or other debris.
6. Push the door closed. It latches automatically.

GeneChip™ Hybridization Oven 645i User Guide 9
Chapter 1 The Hybridization Oven 645i
Unpacking and setup 1
Rear panel items The following items are located on the rear panel (Table 2).
Operating the door 1. The door is hinged on the left of the unit and latches on the right. It opens with a
firm pull on its handle, located at the right side of the door.
2. When the door unlatches, if the oven is ON, the Door Open indicator will
illuminate, rotisserie rotation will stop and the heater will shut off.
3. The door swings open up to 180°, allowing easy access to the oven interior. Take
care not to forcefully overextend the door and ensure that the bench is clear of
objects that may be hit by the door.
4. Take care when opening the door while the oven is operating. The interior is hot.
Avoid immediate contact with the interior or wear protective gloves.
5. To close the door, swing it shut and press the door until it latches. Normal
operation will resume.
Table 2 Rear panel items.
Item What it does How to use
Power entry module /
line filter / fuse holder
Accepts power cord for providing line power to
unit. Filters incoming and outgoing power line
noise. Holds fuses for overcurrent protection.
Plug mating end of power cord into this
connector. Plug other end into suitable
power outlet.
Use your fingers or a screwdriver to
open the fuse holder to replace the two
fuses (See the section,"Replacing the
fuses" on page 22).
Voltage selector
You can manually change the oven voltage from
115V to 230V.
With a screwdriver, flip the switch to
select the required voltage.
Caution! Take care to set the correct
voltage for the oven. An incorrect
voltage setting will damage the oven.
Serial port
The serial port allows communication with the
workstation.
Connect the oven with the serial port of
the computer (RS-232).

Chapter 1 The Hybridization Oven 645i
Using the rotisserie
10 GeneChip™ Hybridization Oven 645i User Guide
1
Using the rotisserie
Installing the
rotisserie into the
oven
Follow this procedure to install a rotisserie into the oven.
1. Set the power switch off and open the oven door.
2. Remove the rotisserie from the accessory box. Note that one end is equipped with
a rubber “foot” (Figure 2). The other end is equipped with a gear (Figure 5).
3. Place the rotisserie into the oven with the rubber mounted shaft end on the left
(Figure 4).
4. Insert the shaft into the holder. Line up the left side of the shaft with the pivot
point, then push the rotisserie to the left against the spring. Press the rotisserie
axle into the receptacle with sufficient force to displace the socket (Figure 3 and
Figure 4).
Figure 2 Detail of the left rotisserie axle, rubber foot.
Figure 3 Detail of the spring-loaded socket.

GeneChip™ Hybridization Oven 645i User Guide 11
Chapter 1 The Hybridization Oven 645i
Using the rotisserie 1
5. Mount the right axle of the rotisserie into the right holder. Place the geared end
of the axle into the right-side drive socket (Figure 5). Turn the rotisserie until the
axle clicks into place. Release the rotisserie to allow the shaft to be seated into the
socket. Gently rotate the shaft to verify that it is moves freely and is installed
correctly (Figure 6).
Figure 4 Installing the rotisserie into the left socket.
Figure 5 Installing the rotisserie on the right holder.

Chapter 1 The Hybridization Oven 645i
Using the rotisserie
12 GeneChip™ Hybridization Oven 645i User Guide
1
Electrical power supply and voltage selection
•Determine the electrical voltage and style of appropriate grounded line cord for
your location.
• On the rear panel of the oven, locate the red 115/230 voltage selector switch
(Figure 7).
• If the switch is set correctly for your location, no adjustment is necessary. If you
must set the voltage switch, then using a small flat-bladed screwdriver, move the
switch so that “115” or “230” is visible and this number matches the electrical
supply employed in your location.
•NOTE: Ovens shipped from the factory have a small label covering the power
entry receptacle, warning the user to select the proper voltage:
• The oven comes equipped with a US NEMA 5-15P style power cord with a
ground pin for use in the Americas. If you are using the oven outside the
Americas, substitute an approved grounded power cord for your location. (For
example, within the EU, employ a “Schuko” style CEE 7/7 grounded cord.)
Figure 6 The rotisserie installed in the oven.
Figure 7 The red 115/230 voltage selector.
WARNING! Voltage selection is factory preset to 230V. Select
the proper voltage for your location before connecting power
cord. Operation with improper voltage selection will damage
the unit.

GeneChip™ Hybridization Oven 645i User Guide 13
Chapter 1 The Hybridization Oven 645i
Operating the oven 1
• After correct voltage and power cord are selected, remove the label covering the
power entry receptacle and connect the power cord to the oven and an electrical
power outlet (Figure 8).
Operating the oven
The front panel The operation of the oven is intuitive and simple. The front panel contains all user
controls (Figure 9).
Figure 8 The 3-pronged plug power receptacle.
Figure 9 The front panel indicators and controls.

Chapter 1 The Hybridization Oven 645i
Operating the oven
14 GeneChip™ Hybridization Oven 645i User Guide
1
System status
indicators
The front panel of the oven contains the system status indicator (Figure 10). Yellow
indicates that the set point has not yet reached the set value. Green indicates that the
set point has been reached and that the oven is operating normally. The indicator
monitors the following items:
•Normal – the green indicator illuminates when the oven temperature is near its
programmed set point.
•Heating – the yellow indicator illuminates whenever Normal status has not yet
been achieved. When started cold, this indicator will remain illuminated while
the oven is warming up (typically about 30 minutes).
•Error – the red indicator illuminates if a functional error has been detected. An
audible alarm will sound and an error code will appear on one of the
alphanumeric displays. For example, if the oven doesn't reach temperature in a
reasonable amount of time, you will get an E02 error and a red Error light. Refer
to the section, "Error codes and troubleshooting" on page 21 for a list of error
codes and their meaning.
•Door Open – the red indicator illuminates whenever the door is open. When the
door is open, the rotisserie will stop and the heater will shut off. When the door is
closed, both functions will resume.
Figure 10 The system status indicator lights.

GeneChip™ Hybridization Oven 645i User Guide 15
Chapter 1 The Hybridization Oven 645i
Operating the oven 1
Applying power
and programming
the oven
1. Verify that the rotisserie is installed and the door is closed.
2. On the left side of the front panel, locate the recessed power switch (the I/O switch
as shown in Table 1 on page 5). The power is OFF when the lower “O” end is
pushed in. The power is ON when the upper “I” end is pushed in.
3. Push the upper end of the switch in to turn power ON. There will be an audible
beep, the two alphanumeric displays will illuminate and briefly display status
information, then scroll “PAUSE”. At this point, the rotisserie is halted and the
oven heater is off.
4. Locate and press the PAUSE key. The scrolling PAUSE indications will
disappear. The current temperature and rotation set points will appear, blinking
in the displays, followed by the actual temperature and rotation status. The
rotisserie may start to turn.
5. Locate the rpm display and UP/DOWN keys (Figure 11). Press the UP and
DOWN keys to change the desired rotisserie rotation rate. Note that the range is
10 rpmto 80 rpm and that programming below 10 rpm turns the controller OFF,
halting the rotisserie. When the UP and DOWN keys are released, the desired
rotation rate blinks for five seconds, then the actual rotation rate is displayed.
6. Press the PAUSE key to halt rotisserie rotation. Press it again to restart rotisserie
rotation.
7. Locate the temperature °C display and UP/DOWN keys (Figure 12). Press the UP
and DOWN keys to change the oven set point - the desired temperature. Note that
the set point range is 30.0°C to 70.0°C and that programming below 30.0°C turns
the temperature set point OFF and turns off the oven heater. When the UP and
DOWN keys are released, the set point value blinks for 5 seconds, then the actual
oven temperature is displayed in degrees centigrade.
Figure 11 The rpm display and UP/DOWN keys.

Chapter 1 The Hybridization Oven 645i
Operating the oven
16 GeneChip™ Hybridization Oven 645i User Guide
1
8. Note that the oven may take about 30 minutes to reach the set point temperature.
9. If power is turned off, when restarted the oven remembers the temperature set
point and rpm rate.
Loading the
rotisserie
1. Load GeneChip™ array cartridges into cartridge carriers. The drive mechanism of
the oven works best under balanced loads, so best practice is to distribute the
cartridges in approximately equal numbers among an even number of carriers.
That is, you should arrange the carriers 180° apart to balance the rotisserie
(Figure 17).
2. Open the oven door. If the oven is in operation, take care that the interior may be
hot.
NOTE: Do not manually rotate the rotisserie. Use the jog (PAUSE) button.
3. While the door is open, the PAUSE button changes to a JOG function. Pressing
PAUSE will cause the rotisserie to slowly rotate. Use this jog feature to rotate the
rotisserie to new positions.
NOTE: There is no on/off button for the rotisserie. You can stop the rotisserie by
opening the door or pressing the Pause button. When the door is open, you can
use the rotisserie Pause button to jog the rotisserie. Do not manually rotate the
rotisserie. For best operation, you should load the cartridge carriers
symmetrically to balance the rotisserie.
4. Use the jog feature to rotate the rotisserie so that an empty position faces the front
of the unit. Install the cartridge carrier then press the carrier retaining clip until it
snaps in place.
5. Install the carrier retaining clips onto the approximate center of crossbars
(Figure 13). These clips are needed to retain each carrier in the rotisserie. One clip
is needed for each carrier.
NOTE: After you install the carrier retainers, they should remain in the oven for
reuse. They simply clip onto the rotisserie crossbar when no carriers are present.
Figure 12 The temperature °C display and UP/DOWN keys.

GeneChip™ Hybridization Oven 645i User Guide 17
Chapter 1 The Hybridization Oven 645i
Operating the oven 1
6. Slide in a cartridge carrier. Only one direction is possible. (Figure 14)
7. Clip the carriage retainer over the crossbars and the carrier (Figure 15).
Figure 13 Installing the carrier retaining clips at the center of the crossbars.
Figure 14 Slide the carrier into the rotisserie.

Chapter 1 The Hybridization Oven 645i
Operating the oven
18 GeneChip™ Hybridization Oven 645i User Guide
1
8. Snap tight the carrier retainer on to the carrier (Figure 16).
9. The rotisserie operates best when the load is balanced. For example, place carriers
at opposing (180°) positions (that is, 3 o'clock and 9 o'clock) on one side of the
rotisserie. If additional carriers are loaded, do the same on the other side. Up to
8 carriers may be loaded. Temperature accuracy and uniformity are not affected
by array or carrier placement (Figure 17).
Figure 15 Clip the carrier retainer over the crossbars and the carrier.
Figure 16 Snap tight the carrier retainer on to the carrier.

GeneChip™ Hybridization Oven 645i User Guide 19
Chapter 1 The Hybridization Oven 645i
Operating the oven 1
10. Close the oven door, set the temperature and set the rotisserie speed if needed.
11. At the conclusion of processing, open the oven door. The rotisserie stops and the
heater shuts off. Wait for the oven surfaces to cool to a comfortable temperature,
then unload the rotisserie.
Unloading the
rotisserie
• Open the door and use the rotisserie motor pause button to jog the rotisserie so
that a carrier faces the front of the unit. The temperature warning light turns on.
This can be ignored.
• Pull on the ring of the retainer clip to release the carrier. Pull it straight out of the
rotisserie.
• Use the jog feature and repeat.
• The retainer clips can remain on the rotisserie. They do not need to be removed.
• The oven is ready for a new load of arrays. If the oven will not be used
immediately, turn the power switch off.
Figure 17 Installed cartridge carriers with the retaining clips set on the carriers.
Note that the carriers are placed 180° apart to balance the rotisserie.

Chapter 1 The Hybridization Oven 645i
Communicating with the oven
20 GeneChip™ Hybridization Oven 645i User Guide
1
Communicating with the oven
Serial
communication
The Hybridization Oven 645i is equipped with a 9 pin connector RS-232 serial port,
located on the rear panel. One 5 meter cable is provided in the oven accessory kit. Tx,
Rx and GND are the active pins employed by the oven. This cable may be connected
to any standard RS-232 COM port on a computer workstation. The communications
protocol is governed by the following rules:
• To communicate with the oven, employ any communication utility (i.e.,
Hyperlink) set up with a Baud rate of 9600 bits/ second, 8 data bits, no parity and
one stop bit (9600, 8, N, 1).
• Communications will occur in half-duplex mode - the oven will accept only one
query and will not accept new queries while acting on a current query.
• All query strings must be no more than 32 characters.
• Each query string starts with a “.” (ASCII hex 2E) character, followed by a three-
character query, ending with a carriage return (ASCII hex 0D).
• Each reply will end with a single byte checksum in hex format and a carriage
return character. The checksum is simply the byte-wise addition of all preceding
characters in the response string.
• Communications are case-sensitive. All queries employ upper case characters.
Queries and responses:
• QSS - Query System Status
– The response is “Eaa,bbb,ccc,dd,ee,f,gg”
– aa = error code. If no error condition exists, this string is “00”
– bbb = temperature set point in tenths degree Celsius
– ccc = actual chamber temperature in tenths degree Celsius
– dd = rotation set point in rpm
– ee = actual rotation rpm
– f = door state (0 = open, 1 = closed)
– gg = hex checksum byte
• QSN - Query Serial Number
– The response is “1829aaaaaaaaa”
– aaaaaaaaa = the last nine digits of the oven serial number
• QSV - Query Software Version
– The response is “a.aa,bb”
– a.aa = software version number
– bb = hex checksum byte
• In case of a syntax error in a query, the oven will reply with the communication
error string “E22”