ThermoFisher Scientific SpeedVac SPD130DLX User manual

Installation and Operation
80302133 • Revision A • July 2018
SpeedVac™ SPD130DLX
Vacuum Concentrator

IMPORTANT: Read this instruction manual. Failure to follow the instructions in this manual can result in
damage to the product, injury to operating personnel, and poor equipment performance.
CAUTION: All internal adjustments and maintenance must be performed by qualified service personnel.
DISCLAIMER: Thermo Fisher Scientific Inc. does not accept any responsibility for any damage caused to its
products by unauthorized personnel.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this document
is for reference purposes only. System configurations and specifications in this document supersede all
previous information received by the purchaser.
© 2018 Thermo Fisher Scientific Inc. All rights reserved.

Contents
Introduction ................................................................. 1
Safety Precautions....................................................... 2
Operating Standards.................................................... 3
Product Specifications ............................................. 3
Environmental Conditions......................................... 3
Installation.................................................................... 4
Contents .................................................................. 4
Installation of SPD130DLX........................................ 4
Operation..................................................................... 5
Description Of Control Panel .................................... 5
Manual Run.............................................................. 7
Auto Run - Time....................................................... 8
Auto Run - Vacuum.................................................. 8
Drying Rate .............................................................. 8
Preset Program Settings .......................................... 9
Exporting Live Run Data........................................... 9
HyperTerminal Configuration .................................... 10
Lid Stay Mechanism................................................. 10
Other Topics ............................................................ 10
Rotors...................................................................... 12
Accessories ............................................................. 12
Appendix 1 .................................................................. 14
Additional SPD130DLX System Setup...................... 14
Warranty ...................................................................... 15

SpeedVac™ SPD130DLX Introduction | 1
Introduction
Thermo Scientific Savant SpeedVac™ SPD130DLX vacuum
concentrator offers fast, reliable processing of samples. It
effectively removes a broad range of aggressive and volatile
solvents to concentrate or dry solutes, analytes and residues
while providing complete sample recovery.
The combination of vacuum, applied to the sample chamber
from an external source, and centrifugal action promotes
solvent evaporation without sample loss due to foaming or
bumping. The evaporation process is accelerated with the
addition of chamber heat to counteract the extreme
evaporative cooling of the samples. The concentration
process is completed with the addition of a vapor
condensation trap available separately for virtually 100%
solvent recovery. This results in a thorough evaporation
process in a safe environment for both the sample and the
user. The unit features an advanced front panel with dual
timer for automatic or manual control of run conditions, it also
features preset and user defined programs which enable user
to retrieve and store application protocols for future use.
The compact, industrial strength SPD130DLX features:
• Ultimate chemical resistance to aggressive solvents with
the following provisions:
- Components made of Teflon®and Teflon®coated
materials used exclusively in the vapor path.
- Dual valve manifold positioned to reduce solvent
vapor exposure.
- Dedicated bleed port added to assure unidirectional
vapor flow and to purge bearings with fresh air.
• Faster evaporation rate with the following enhancements:
- Glass cover with lamps apply radiant heat to
samples.
• Microprocessor control of run and heat times offer fully
unattended, automatic operation.
• Built-in digital vacuum gauge allows continuous
monitoring of process.
• Vacuum based run terminates run when pre-set vacuum
level is reached.
• 3 preset programs pre-loaded with default application-
based run parameters.
• 3 user-defined programs for custom application-based
run parameters that enables user to retrieve and store
protocols for future use.

2 | Safety Precautions SpeedVac™ SPD130DLX
Safety Precautions
In this manual, the following symbols and conventions are
used:
Below are important safety precautions that apply to this
product:
This symbol when used alone indicates
important operating instructions which reduce
the risk of injury or poor performance of the unit.
CAUTION: This symbol, in the context of a
CAUTION, indicates a potentially hazardous
situation which if not avoided could result in
minor to moderate injury or damage to the
equipment.
WARNING: This symbol, in the context of a
WARNING, indicates potentially hazardous
situations which, if not avoided, could result in
serious injury or death.
This symbol indicates situations where
dangerous voltages exist and potential for
electrical shock is present.
The snowflake symbol indicates extreme low
temperatures and high risk of frostbite. Do not
touch bare metal or samples with unprotected
body parts.
This symbol indicates possible pinch points
which may cause personal injury.
This symbol indicates a need to use gloves
during the indicated procedures. If performing
decontamination procedures, use chemically
resistant gloves.
Before installing, using or maintaining this
product, please be sure to read the manual and
product warning labels carefully. Failure to follow
these instructions may cause the product to
malfunction, which could result in injury or
damage.
This symbol represents protective conductor
terminal.
WARNING: Disconnect the unit from all power
sources before cleaning, troubleshooting, or
performing other maintenance on the product or
its controls.
WARNING: Do not use this device in
radioactive, highly reactive or explosive
atmosphere.
Do not use this device to process any explosive,
radioactive, highly reactive or explosive
atmosphere creating substances.

SpeedVac™ SPD130DLX Operating Standards | 3
Operating Standards
Product Specifications
*Dependent upon ambient temperature, line voltage fluctuation, and load capacity.
Environmental Conditions
Indoor use only, in the absence of hoarfrost, dew, percolating water, rain and solar radiation.
Main supply voltage fluctuations not to exceed ±10% of the nominal voltage.
Transient overvoltages per Installation Categories II.
Operative Power* 115 VAC; 60 Hz; 6 amps
230 VAC; 50 Hz; 3 amps
Vacuum Chamber TEFLON®coated chemical resistant aluminum casting
Cover Heated cover with radiant lamps - includes a safety interlock
Induction Motor Maintenance free
Bearings Standard
Chamber temperature 35°C to 80°C, 5°C increments
Vacuum Level Control 20 to 0.1 Torr in 0.1 increments
Weight 62 lbs
28 kg
Dimensions (W x D x H) 14 in x 18 in x 14 in
36 cm x 46 cm x 36 cm
Fuse 6 A, 250 VAC, Time-lag
3 A, 250 VAC, Time-lag
Maximum altitude 2000 meters above mean sea level
Ambient temperature range 17°C to 32°C
Humidity 20% to 80% non-condensing
Pollution degree 2

4 | Installation SpeedVac™ SPD130DLX
Installation
Contents
Unpacking. Open the shipping cartons. Carefully remove the
instrument and accessories. Lift and carry with two
people, holding securely underneath with both hands.
Use proper lifting technique (lift with the legs, not the
back) to avoid personal injury. Compare the contents with
the packing list. If there is a discrepancy, call Thermo
Scientific technical service.
Inspection. Inspect the unit and accessories for damage that
may have occurred during shipment. Should there be any
damage, report it to the carrier and contact Thermo Scientific
immediately. Make sure the carrier inspects the damage and
leaves an inspection report. Register any claims for shipping
damage against the carrier or his agent. Save the shipping
carton in the event a return is necessary. Call Thermo
Scientific technical service for further assistance.
Site preparation. The SPD130DLX requires a stable surface
that is clean, dry, level, and within 4 feet (1.2 meters) of a
compatible electrical outlet. Place the unit in a convenient
location with access to a vacuum source and a vapor trap1.
The SPD130DLX units configured for 115 VAC, 60 Hz, should
be plugged into a circuit rated for at least 6 amps. The
SPD130DLX units configured for 230 VAC, 50 Hz, should be
plugged into a circuit rated for at least 3 amps.
Note: Do not use any detachable power cord that is not
adequately rated for the unit.
Installation of SPD130DLX
Figure 1. Rear View of System
Table 1. Contents
Qty. Description
1Thermo Scientific Savant SpeedVac™
SPD130DLX Concentrator
1Teflon®tubing 1/2” I.D. x 3’ long
1Line Cord
1.To prevent damage to pump due to vapor exposures from sample solvent and to extend pump life, a vapor trap that condenses and traps vapors must be
placed in line between the SpeedVac™ concentrator and vacuum pump. We offer integrated universal vacuum systems (integrated vapor trap with the
vacuum pump) or component vacuum system (vapor trap plus vacuum pump).
CAUTION: Be sure to leave at least 4 inch
clearance on all sides of the unit. Overhead
clearance, equal to 18 inch (46cm), is required for
raising the cover. Verify that the unit is on a
leveled and stable platform. If necessary, move
the unit to a more suitable location.
WARNING: Before connecting the unit to an
electrical outlet, make certain that voltage,
frequency, and amperage match the
requirements indicated on the product label,
name plate of the instrument. Use sockets with a
protective earth conductor and correct mains
cable.
CAUTION: Cold trap should be in line between
the vacuum source and the SPD Unit.
Clamps
RVT5105
Refrigerated Vapor
Trap
SPD130DLX
SpeedVac
Concentrator
Vacuum Pump

SpeedVac™ SPD130DLX Operation | 5
Operation
Connect the power cord to the power inlet on the back of the
unit and plug it into the appropriate outlet. Turn on the main
power switch located on the front of the unit in the lower right
to energize the unit. The safety lid locking mechanism on the
SPD130DLX will automatically disengage. Do not attempt to
open the lid unless the unit is plugged in.
Note: The SPD130DLX Concentrator is an important
component in a complete concentration system that should
also include a refrigerated vapor trap and a vacuum pump
along with other applicable accessories. Please refer to
operating instructions of each component for details on
usage.
Rotor installation. Open the lid of the rotor chamber.
Visually align the pin on the drive shaft with the groove on the
bottom of the rotor. Carefully lower the rotor onto the drive
shaft. Rotate the rotor by hand to ensure alignment of the pin
with the groove. Secure the assembly by screwing the
retaining knob into the drive shaft above the rotor. Tighten it
firmly but not excessively.
Description Of Control Panel
1. Lid locked - Indicates whether or not the lid of the
concentrator is locked. The indicator will be illuminated
when locked.
2. Preset buttons – 3 preset programs that are not
modifiable.
Loading a preset is achieved by pressing the
corresponding Preset button. The following parameters
are automatically loaded into the user interface:
• Temperature set point
•Heattime
• Run time
• Vacuum pressure
• Run type
CAUTION: Use appropriate Thermo Fisher
Scientific designed rotors recommended for the
Product.
CAUTION: Load the rotor and close the lid.
Always balance rotor loads. An imbalanced rotor
causes vibration that will damage the system’s
bearings and equipment. Load the rotor
symmetrically. There need not be a tube in each
holder, but the load must be evenly distributed.
When using a rotor with aluminum tube holders,
insert all the tube holders.
1 4 5 6 11 12
1516141310987
23

6 | Operation SpeedVac™ SPD130DLX
3. Program buttons - 3 programs that are modifiable.
Loading a user defined program is achieved by pressing
the corresponding Program button. The following
parameters are automatically loaded onto the screen:
• Temperature set point
• Heat time
• Run time
• Vacuum pressure
• Run type
Saving a user defined program is achieved by pressing
and holding down the corresponding Program button for
3 seconds. The parameters displayed in the screen are
stored into the program and can be loaded for use at a
later time.
4. Temperature display - Indicates the set temperature or
the actual temperature during a run in °C. The
temperature set point can be set from 35°C to 80°C at a
5°C interval. In addition, it is possible to set the
temperature to “no”, which means that heating will not be
applied during the run. The temperature setting of “no”
can be reached under 35°C or over 80°C.
5. Time display - Indicates the heat timer or the run timer
set points. During a run, it indicates the elapsed or
remaining run time or the remaining heat time left. The
range for these timers is from 0.01 [1 min.] to 9.59 [9
hours, 59 minutes] at a 1-minute interval. In addition, it is
possible to set the heat time to “CCC”, which represents
a continuous heating throughout the execution of a run.
The setting of “CCC” can be reached under 0.01 [1 min.]
or over 9.59 [9 hours, 59 minutes].
6. Vacuum display - Indicates the vacuum pressure set
point (in Torrs or millitors). During a run, it indicates the
actual vacuum pressure.
Note:
• The atmospheric pressure is represented by “HPr”.
• A decimal point indicates a vacuum pressure in Torr.
• No decimal point indicates a vacuum pressure in
mTorr.
• When the Run Type is set to TIME, the Vacuum level
will display “---” is shown until a run is started.
7. Select - Press this button to select the parameters to be
modified. The selection is from left to right, in a cyclic
manner. The selected parameters is shown by the
corresponding indicator that is illuminated.
8. Minus (“-”) - Decrements the value of the selected
parameter.
9. Plus (“+”) - Increments the value of the selected
parameter.
10. View - During a run, pressing view shows the set run
parameters. When no run is ongoing, pressing view
displays the actual live parameters. The time display
shows either “0.00” or the end status of the last executed
run (e.g. “End”, “Err.”)
11. Pre-Heat - Use to pre-heat the chamber to 45 °C prior
to beginning a run or between runs. Once a run is
initiated, the pre-heat automatically stops. Note that the
pre-heat function is only enabled if the temperature set
point is different than “no”.
12. Run Type – Selects one of the 2 following types of run:
• “Time”: Time-based run that does not use vacuum
control when a run is ongoing. The vacuum will be
the maximum allowed by the attached external
vacuum pump, regardless of the vacuum pressure
set point.
• “Vac”: Vacuum-based run that, in the context of an
automatic run, will automatically terminate the run
when the vacuum set point is reached.
The selected run type will be shown by the corresponding
illuminated indicator.
13. RL on/off - Adds radiant heat to chamber by activating
the radiant lamp of the Lid. The radiant lamp can be
activated and deactivated manually by pressing the
button at any point during a run. The indicator will be
illuminated accordingly. If enabled, the radiant lamp will
remain powered as long as the heat timer has not
expired. Note that the feature is disabled if the
temperature set point is set to “no”.
14. Manual run - Starts a "Manual" run based on the
parameters loaded in the screen, use manual run
function if you do not want to set a fixed run time.
15. Auto run - Starts an 'Automated' run based on the
parameters loaded in the screen, use auto run function if
you want to set a fixed run time.
• Time-based run: Terminates run after the run time
has expired
• Vacuum-based run: Terminates run after the vacuum
set point is reached
16. Stop - Terminates a "Manual" or "Auto" run.
CAUTION: Do not use radiant heat with
microplates. Improper use may result in melting
or deformation.

SpeedVac™ SPD130DLX Operation | 7
Audible Notification
The concentrator will play tones depending on the user
actions. Below is a description of these tones.
Manual Run
1. Connect the unit to its required voltage.
2. Turn the power switch located on the front of the UNIT, to
the ON position, (light on switch indicates ON). The cover
lock disengages, allowing the top cover to be opened.
The display lights up and shows the following in
sequence:
• Name of the concentrator
• Software revision number
• Default values:
- Run Type Time
- Temperature 35°C
- Run Time 2.00 hours
- Vacuum 1 Torr
3. Select a run configuration using one of the following
methods:
• Load a preset program
• Load a user defined program
• Directly modify values that are loaded on the user
interface, using the Select button and the “+”/ “-”
buttons.
4. Set the temperature set point between 35°C and 80°C,
or “no”, for no heat.
5. Using the Select button and the “+”/ “-” buttons select
and modify “Heat Time” to between 0.01 and 9.59 hours
or “CCC” (for continuous heating). When the heat timer
expires, the heater will shut off, no matter what the
temperature set point reads (except if “CCC”).
6. Select Run Time: Since this is a manual run, no time
adjustment is needed.
7. Select a vacuum set point: Since this is a manual run, the
vacuum will be the maximum allowed by the attached
external vacuum pump, regardless of the vacuum
pressure set point.
8. Select the Run Type: Since this is a manual run, the run
type setting is ignored. The vacuum will be the maximum
allowed by the attached external vacuum pump,
regardless of the vacuum pressure set point.
9. Place sample tubes in rotor so load is balanced. Secure
rotor with the supplied knob (hand tight). Close cover.
10. Pre-heat may be selected at this time, to apply a 45°C
heat to the chamber.
11. Press the Manual Run button. The cover locks and the
lid locker indicator is illuminated. The rotor starts
spinning. The “Run Time” display counts up. The
temperature rises to the set temperature. The “Heat
Time” will count down if set point is not set to “CCC” and
if the temperature set point is not “no” The vacuum will be
applied to the chamber and the level begins falling.
Note: If the cover is not closed, the display will show “Lid”
and the run will not start.
12. Press RL On/Off for radiant chamber heat. Press at any
time to turn ON and OFF. (As long as there is time left in
the heat timer and if the temperature set point is not set
to “no”).
13. To end the manual run, press Stop button. The unit will
sound three audible beeps. The display will show “End”,
the valves will click, isolating the chamber from the
vacuum pump and allowing air to bleed into the chamber.
14. After the rotor stops spinning, the cover lock disengages
and the lid locked indicator is turned off. The display
reverts to last set parameters.
15. Open the cover and remove samples.
GENERAL: During the run, display shows actual parameters.
To check set parameters, press View button and Select. The
Table 2. Audible Notifications
Event Sound Description
Start-up 5 beeps
Starting a run (manual
or automatic) 1 single beep
Manually stopping a
manual or automatic
run
3 beeps
Automatically
stopping an automatic
run
1 beep that is repeated until the
user pushes the Stop button or
opens the cover of the
concentrator
Program saved 3 beeps
User input error 1 long beep that lasts for 1
second
System error
1 long beep that lasts for 1
second and that is repeated
until the user pushes the Stop
button or opens the cover of the
concentrator

8 | Operation SpeedVac™ SPD130DLX
display will revert temporarily to set points for approximately 5
seconds.
Auto Run - Time
1. Refer to the Manual Run section for start-up.
2. To execute an Automatic time-based run, execute the
following steps:
a. Select the Run Type as “Time”.
b. Use the Select button and the “+”/ “-” buttons to
select and modify the Temperature, “Heat Time” and
“Run Time” parameters. The Run and Heat Time can
be set from 0.01 to 9.59 hours (Heat Time also has
“CCC” for continuous use).
c. Select a vacuum set point: Since this is a time-based
run, the vacuum will be the maximum allowed by the
attached external vacuum pump, regardless of the
vacuum pressure set point.
d. Press the Auto Run button to start the run. The
cover locks and the lid locked indicator is lit. The rotor
starts spinning. The run time display is counting
down in 1 minute Intervals. The heat time is counting
down if the set point is not set to “CCC” and if the
temperature set point is not set to “no” (use the
select button to view “Heat Time”). The temperature
rises in 1 °C increments to set temperature. The
vacuum is applied to the chamber and the vacuum
pressure begins decrementing down from “HPr”
(atmospheric pressure).
Note: If the cover is not closed, the display will show
“Lid” and the run will not start.
e. Press RL On/Off for radiant heat. Press at any time
to turn ON and OFF. (As long as there is time left in
the heat timer and if the temperature set point is not
set to “no”).
f. Once the time expires, the run will automatically stop,
the display will show “End”, the SAV valves will click,
also allowing air to bleed into the chamber. The unit
will sound 1 beep that will be repeated until the user
acts on it.
g. After the rotor stops spinning, the cover unlocks and
the lid locked indicator is turned off. The display
reverts to last set parameters.
h. Open the cover and remove samples.
Auto Run - Vacuum
1. Refer to the “Manual Run” section for start-up.
2. To execute an Automatic Vacuum run, execute the
following steps:
a. Select the Run Type as “Vac”
b. Use the Select button and the “+”/ “-” buttons to
select and modify the Temperature, “Heat Time and
“Run Time” parameters. The Run and Heat Time can
be set from 0.01 to 9.59 hours (Heat Time also has
“CCC” for continuous use).
c. Select a vacuum set point at which the run should be
terminated, ensure the attached external vacuum
pump can generate the required vacuum level
d. Press the Auto Run button to start the run. The
cover locks and the lid locked indicator is lit. The rotor
starts spinning. The run time display will count up in 1
minute intervals. The heat time is counting down if
the set point is not set to “CCC” and if the
temperature set point is not set to “no” (use the
Select button to view “Heat Time”). The temperature
rises in 1 °C increments to set temperature. The
vacuum is applied to the chamber and the vacuum
pressure begins decrementing down from “HPr”
(atmospheric pressure).
Note: If the cover is not closed, the display will show
“Lid” and the run will not start.
e. Press RL On/Off for applying radiant heat. Press at
any time to turn ON and OFF. (As long as the heat
timer has not expired, and if the temperature set
point is not set to “no”).
f. Once the unit senses the set vacuum pressure in the
chamber has been achieved, the run will
automatically stop and the display will show “End”.
The valve will click, allowing air to bleed into the
chamber. The unit will sound 1 beep that will be
repeated until the user acts on it.
g. After the rotor stops spinning, the cover unlocks and
the lid locked indicator is turned off. The display
reverts to last set parameters.
h. Open the cover and remove samples.
Drying Rate
Thermal energy can be applied to the concentrator chamber
to counteract the cooling effect of evaporation, maintain the
samples in the liquid state, and accelerate the concentration
run. Select the highest drying rate if you desire accelerated

SpeedVac™ SPD130DLX Operation | 9
drying. To ensure against over drying of samples, and
possible denaturing, it is recommended that the “Heater”
timer and the Radiant heat be set for only a portion of the total
run time (e.g., 50%-75% of total run time).
Preset Program Settings
The table below shows the values attributed to the preset
programs parameters.
*When the temperature is set to “no”, the heat time is
defaulted to “0.00” while executing the run.
**When the Run Type is set to TIME, the Vacuum level will
display “---” is shown until a run is started.
Exporting Live Run Data
The unit is equipped with a USB port to enable
communication with an external computer. The USB port is
located on the rear panel of the unit and is connected to a
computer via a standard USB type A to B cable.
Figure 2. USB Port location
When a run is ongoing, the unit exports the following
information via the USB port every minute.
• The live temperature applied to the samples chamber
• The remaining heat time for the run (“CCC” indicates
continuous heating)
• The remaining or elapsed run time, depending on the run
type (manual or automatic)
• The vacuum pressure in the samples chamber
The data are sent at a fixed baud rate of 115200 and are
comma-separated as shown in the format: <temperature>,
<heat time>, <run time>, <vacuum pressure>
• The temperature is represented as a 2-digit integer in °C
• The heat time is expressed as a 3-digit integer in minutes
(or “CCC” for continuous heating)
• The runtime is expressed as a 3-digit integer in minutes
• The vacuum pressure is represented as a floating-point
number in Torr (or as “HPr” to represent a high pressure).
Note: The following section details the step-by-step
procedures for configuring Microsoft®HyperTerminal running
on a host computer using Windows®XP. These instructions
may need to be modified to be used with a different terminal
emulator program and/or operating system. Contact
Technical Services if further assistance is required.
Table 3. Preset Program Settings
Preset Application Settings
Preset 1 Water
Temperature: 45°C
Heat time: 2.00
Run time: 2.00
Vacuum level: 1.0 Torr
Run Type: VACUUM
Preset 2 “Low Boiling”
ACN/MEOH
Temperature: 65°C
Heat time: 2.00
Run time: 2.00
Vacuum level: 100 mTorr**
Run Type: TIME
Preset 3
HPLC
H2O + Low Boil
solvent +.1% acid
Temperature: “no”
Heat time: 0.01*
Run time: 2.00
Vacuum level: 100 mTorr**
Run Type: TIME
Note: The preset programs are for convenience
only. Prior to use, users are advised to conduct a
risk assessment to confirm that the preset
program parameters meet the user’s application
specific needs and conditions. Users accept the
risks associated with using the preset programs.
CAUTION: USB lead length less than 3 meters
shall be used to connect to the computer.
In case of a communication failure between the
monitoring program of the computer and the
product, user can re-establish the connection by
disconnecting and reconnecting the USB cable.

10 | Operation SpeedVac™ SPD130DLX
HyperTerminal
Configuration
1. Power up the host computer and close any running
applications.
2. Open the HyperTerminal application by clicking on Start \
“Programs” \ “Accessories” \”Communications” \
“HyperTerminal.”
3. In the “Connection Description” box, enter the name
“SPD130DLX” and choose an icon and click OK.
4. Connect to the virtual COM port that is linked with the
SpeedVac™ concentrator attached to the computer
(USB virtual com port).
5. In the “COM Port Properties” box \ “Port Settings” folder
select the following options:
Bits per second: _ 115200
Data bits: _ 8
Parity: _ None
Stop bits: _ 1
Flow control _ None
After verifying the above settings, click OK.
6. In the main dialog box click on File \ Save.
7. Exit the program by clicking on File \ Exit \ Yes.
8. Verify the program was saved by going to Start \
“Programs” \ “Accessories” \ “Communications” \
“HyperTerminal” \ “SPD130DLX”.
9. This completes the configuration of HyperTerminal.
10. Turn on SpeedVac™ system and connect to the virtual
COM port that is linked with the SpeedVac™ attached to
the computer.
11. Start HyperTerminal by clicking on SPD130DLX.
12. Initiate the run and SpeedVac™ will screen print
<temperature>, <heat time>, <run time>, <vacuum
pressure>
Lid Stay Mechanism
The unit is equipped with a lid-stay mechanism that will assist
the user in opening and closing the cover of the unit. It will
secure the lid and maintain it open, allowing the user to
operate the unit with both hands.
Figure 3. Lid stay mechanism view
Other Topics
Chamber Cover. The cover must be closed before
beginning a run. If you press Manual Run or Auto Run and
the cover is open, the display shows “lid” to remind you to
close the cover. The run will start immediately after closing the
cover.
If a user tries to open the lid at the start of the run, when the
lid has already been locked, the unit will automatically abort
the run and signal the user with the “Lid” message on the
display and an error tone. Otherwise, during a run the user
cannot open the cover due to vacuum in the concentration
chamber. The cover is locked down at all times during a run
and whenever power to the unit is interrupted.
The cover lock is an additional safety feature that reduces the
risk of injury or damage from the spinning rotor. Do NOT
bypass the cover lock to conduct a run with the cover open.
If removal of samples from the concentrator chamber during
a power failure is required, remove the screw holding the
cover lock release cord and lightly pull it while lifting the cover.
The cover can then be opened.
Figure 4. Opening chamber cover in emergency
WARNING: Device lid can crush your fingers.
Keep away: Do not reach between the device
and lid when opening or closing the device lid.

SpeedVac™ SPD130DLX Operation | 11
In the event that your choice of solvents and applications are
unique and not listed above, please contact Thermo Scientific
Customer Support for advice.
Routine checks:
1. Check all the hoses to ensure that they are secure.
2. Ensure all glass condensation flask in emptied, cleaned
and checked for crack before every run.
3. Clean the cover, cover seal and chamber with a soft lint
free cloth with one of the following
• Mild detergent solution
• Diluted Methanol (50%)
• Diluted Ethanol (50%)
CAUTION:
List of Solvent and Solvent Combinations:
Solvent & Solvent Combination
Water+ACN+MeOH+low concentration of formic
acid(1%)
Water+ACN+MeOH+low concentration of formic
acid(1%)0.1% Trifluoroacetic acid
Acetonitrile
Acetone
Acetic Acid
0.1% Acetic Acid
Ammonium hydroxide
n-butyl alcohol
Chloroform
Cyclohexane
DCM
Ethyl acetate
Ethanol
Ethyl ether
n-Hexane
n-Heptane
Isopropyl alcohol
Isobutyl alcohol
Methanol
Petroleum ether
n-propyl alcohol
Pyridine
0.1% Trifluoroacetic acid
WARNING: Do not use this device in radioactive,
highly reactive or explosive atmosphere.
Do not use this device to process any explosive,
radioactive, highly reactive or explosive
atmosphere creating substances.
CAUTION: Product Maintenance:
Maintenance of product only permitted to be
performed by trained service staff using suitable
measuring and auxiliary equipment as well as
detailed service instruction.
CAUTION: Wear gloves, eye protection, masks
and lab coats while working on the system.
WARNING: Burns due to hot device parts:
When the concentrator is set to a temperature of
60°C and above the rotor and the sample tubes
will be hot, do not touch the wall of the rotor
chamber, use suitable auxiliary to remove the
sample tubes

12 | Operation SpeedVac™ SPD130DLX
Rotors
Traditional Rotors for SPD130DLX Vacuum Concentrator
Accessories
Sample Format
Ty p e
Working
Volumes (ml) Number Description Traditional Rotor Model
MICROCENTRIFUGE
TUBES
1.2 - 1.6 40 1.5 -2.0 ml RH40-11
1.2 - 1.6 64 1.5 -2.0 ml RH64-11
1.2 - 1.6 120 1.5 -2.0 ml RH120-11
3.5 10 17 X 60 mm (5 ml) RH10-15
GLASS AND PLASTIC
TUBES
0.3 100 0.4 ml (96 X 50 mm) RH100-6
0.3 100 0.5 ml (8 x 29) RH100-8
440 1.5 - 20 ml (12 x 75mm) RH40-12
472 12 X 75mm RH72-12
832 13 X 100mm RH32-13
15 817.5 X 102 mm, 15ml Corex tubes RH8-17.5
10 818 X 100 mm, 17 x 95, 16 x 100 RH8-18
CENTRIFUGE TUBES
12 10 15ml conical (16 x 120 mm) RH10-15
40 650ml conical (28 x 115 mm) RH6-50
FLASKS 80 4100 ml pear shaped flask RH4-100
VIALS
260 12 x 32 mm, 12 x 40 mm RH60-12-40
324 1 dram vials, 15 x 45 mm (4ml) RH24-15
2.4, 4 12 20 x 47 mm, 20 x 60 mm RH12-20
5.6 24 18 x 52 mm scintillation vial RH24-18
16 12 28 x 60 mm, 20ml scintillation vial RH12-28
MICROWELL PLATES
2Shallow well plates RHDW2MP
6Shallow well plates RHSW6MP
Part No. Description
GCF400 Glass condensation flask
FC400 Flask cover for GCF400
145-6012-00 Foam insulating ring
SCC1* CryoCool®heat transfer fluid (1 liter)

SpeedVac™ SPD130DLX Operation | 13
*Hazardous Materials - These items require special shipping/handling when shipped by air.
ANT100 Post-trap assembly for Oligo Preps - Used on UVS850DDA only
ANS121 Ammonia neutralizing solution for Oligo Preps - Used on UVS850DDA only
SCT120 Chemical trap (order cartridges separately)
DTK120R Chemical trap kit for radioactivity (compatible with all Thermo vacuum concentrator products)
DC120A Disposable cartridge for neutralizing acid
DC120R Disposable cartridge for trapping volatile radioactivity
CC120/DX Deluxe convenience cart for SpeedVac™ systems
Part No. Description

14 | Appendix 1 SpeedVac™ SPD130DLX
Appendix 1
Additional SPD130DLX System Setup
Figure 5. Semi-Integrated System
Figure 6. Component High Vacuum System
SpeedVac Concentrator
SPD130DLX
Vacuum Tubing
To Pump
ANT100
Exhaust recovery
Post trap
Vacuum Tubing
From Cold Trap
to Back of
SPD130DLX
UVS850DDA Vacuum Tubing
From Back of
SPD130DLX
To Cold Trap
SpeedVac Concentrator
SPD130DLX
Vacuum Tubing
RVT450
Cold Trap
VLP120
Vacuum
Pump
VPOF110
Vacuum Pump
Oil Filter
DVG50
Vacuum Tubing From Back of
SPD130DLX To Cold Trap
Vacuum Tubing From Cold Trap
To Back of SPD130DLX
Flexible TEFLON
Vacuum Tubing

SpeedVac™ SPD130DLX Warranty | 15
Warranty
All Thermo Fisher Scientific products mentioned in this
manual (excluding glassware) are warranted against defects
in workmanship for one year after the date of delivery to the
original purchaser. This warranty is limited to defective
materials and workmanship and does not cover incidental or
consequential damages.
Thermo Fisher Scientific will repair free of charge any
apparatus covered by this warranty. If a new component fails
to work, Thermo Fisher Scientific will replace it, absorb all
charges, and continue the one-year warranty period.
Warranty work is subject to our inspection of the unit. No
instruments, equipment, or accessories will be accepted
without a Return Material Authorization (RMA) number issued
by Thermo. Costs of shipping the unit are not covered under
warranty. The warranty obliges you to follow the precautions
in this manual.
When returning apparatus that may contain hazardous
material, you must pack and label them following U.S.
Department of Transportation (DOT) regulations applying to
transportation of hazardous materials. Your shipping
documents must also meet DOT regulations. All returned
units must be decontaminated (free of radioactivity,
biological, or chemical contamination).
Use of this equipment in manners other than those specified
in this manual may jeopardize personal safety. Under no
circumstances shall Thermo Fisher Scientific be liable for
damages due to the improper handling, abuse, or
unauthorized repair of its products. Thermo Fisher Scientific
assumes no liability, express or implied, for use of this
equipment.
Visit us online to register your warranty:
www.thermoscientific.com/labwarranty

WEEE Compliance
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Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. Thermo Fisher Scientific has
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www.thermofisher.com/WEEERoHS under Services & Support.
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getroffen mit Verwertungs-/Entsorgungsanlagen in allen EU-Mitgliederstaaten und dieses Produkt
muss durch diese Firmen widerverwetet oder entsorgt werden. Mehr Informationen über die
Einhaltung dieser Anweisungen durch Thermo Scientific, dieVerwerter und Hinweise die Ihnen nützlich
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