ThermoFisher Scientific TX-150 User manual

Thermo Scientific
Small Benchtop Rotors
for Thermo Scientific Megafuge8/8R,
SorvallST8/8R and SL8/8R Centrifuges
Instruction Manual
50143334-e • 07 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked with the following symbol:

Thermo Scientific Small Benchtop Rotors |3
WEEE Compliance..........................................2
Preface ..................................................7
Intended Use ........................................................... 7
Precautions ............................................................ 7
Symbols Used in the manual .............................................. 8
Thermo Scientific Rotor Specifications ........................9
TX-150 ...............................................................10
Items Supplied ........................................................ 10
Technical Data ........................................................ 10
Accessories .......................................................... 14
Biocontainment Certificate ................................................ 15
TX-100S ..............................................................16
Items Supplied ........................................................ 16
Technical Data ........................................................ 16
Accessories .......................................................... 17
Biocontainment Certificate ................................................ 18
TX-100 ...............................................................19
Items Supplied ........................................................ 19
Technical Data ........................................................ 19
Accessories .......................................................... 20
M10 .................................................................21
Items Supplied ........................................................ 21
Technical Data ........................................................ 21
Accessories .......................................................... 23
Biocontainment Certificate ................................................ 24
MT-12................................................................25
Items Supplied ........................................................ 25
Technical Data ........................................................ 25
HIGHConic III .......................................................... 27
Items Supplied ........................................................ 27
Technical Data ........................................................ 27
Accessories .......................................................... 28
Biocontainment Certificate ................................................ 29
CLINIConic ............................................................30
Items Supplied ........................................................ 30
Technical Data ........................................................ 30
Accessories .......................................................... 31
MicroClick 18 x 5 ...................................................... 32
Items Supplied ........................................................ 32
Technical Data ........................................................ 32
Table of Contents

Table of Contents
4|Small Benchtop Rotors Thermo Scientific
Accessories .......................................................... 33
Biocontainment Certificate ................................................ 33
MicroClick 24 x 2 ...................................................... 34
Items Supplied ........................................................ 34
Technical Data ........................................................ 34
Accessories .......................................................... 35
Biocontainment Certificate ................................................ 36
MicroClick 30 x 2 ...................................................... 37
Items Supplied ........................................................ 37
Technical Data ........................................................ 37
Accessories .......................................................... 38
Biocontainment Certificate ................................................ 39
Microliter 48 x 2 .......................................................40
Items Supplied ........................................................ 40
Technical Data ........................................................ 40
Accessories .......................................................... 41
Biocontainment Certificate ................................................ 42
8 x 8 PCR Strip ........................................................ 43
Items Supplied ........................................................ 43
Technical Data ........................................................ 43
Accessories .......................................................... 44
Biocontainment Certificate ................................................ 45
8 x 50 mL Individually Sealed ............................................ 46
Items Supplied ........................................................ 46
Technical Data ........................................................ 46
Accessories .......................................................... 47
Biocontainment Certificate ................................................ 48
Thermo Scientific
Auto-Lock Rotor Exchange .................................49
Rotor Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Removing the Rotor .................................................... 50
Rotor Loading ............................................51
Before a Run .......................................................... 51
Rotor Temperature Range................................................ 51
Rotor Loading .........................................................51
Correct Balances....................................................... 52
Incorrect Balances...................................................... 52
Maximum Loading .....................................................52
Rotor Life Time ........................................................53
Service Life Examples ................................................... 53
Aerosol-tight Applications..................................55
Basic Principles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Placing O-Ring ........................................................ 55

Table of Contents
Thermo Scientific Small Benchtop Rotors |5
Fill Level.............................................................. 56
Checking the Aerosol-Tightness ..........................................56
Quick Test............................................................ 56
Aerosol-tight Closure with ClickSeal ......................................... 57
Removing of Aerosol-tight Rotors .........................................58
Maintenance and Care.....................................59
Cleaning Intervals ......................................................59
Basics ...............................................................59
Rotor and Accessories Inspection........................................... 59
Cleaning..............................................................60
Disinfection ...........................................................61
Decontamination.......................................................62
Autoclaving ...........................................................63
Service of Thermo Fisher Scientific........................................63
Storage ..............................................................63
Shipping and Disposal ..................................................64
Chemical Compatibility Chart ...............................65
Index ...................................................71

Thermo Scientific Small Benchtop Rotor |7
Before starting to use a rotor, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the
sellers warranty.
Intended Use
This centrifuge is a laboratory product used to separate substance mixtures of different densities.
This centrifuge has to be operated by trained specialists only.
Precautions
In order to ensure safe operation of these Thermo Scientific small benchtop rotors, the following general safety regulations
must be followed:
• Do not remove any components of the rotor.
• Do not use rotors which show any signs of corrosion and/or cracks. Do not touch the electronic components of the
centrifuge and do not make any changes to the electronic or mechanical components.
• Use only with rotors which have been loaded properly.
• Never overload these rotors.
• Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this rule are
commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the
RCF value of the rotor.
• Observe the safety instructions.
Pay particular attention to the following aspects:
• Rotor installation: Check that the rotor is locked properly into place before operating the centrifuge.
• Always balance the samples.
Maximum sample density at maximum speed: 1.2
Preface
P

Symbols Used in the manual
8|Small Benchtop Rotors Thermo Scientific
Symbols Used in the manual
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific Small Benchtop Rotors |9
Thermo Scientific Rotor
Specifications
There are several Thermo Scientific™small benchtop rotors available for the Thermo Scientific Megafuge™ 8 / 8R
centrifuge, the Thermo Scientific Sorvall™ST 8 / 8R centrifuge and the Thermo Scientific SL 8 / 8R centrifuge.
Thermo Scientific Rotors Article No.
TX-150 swinging bucket rotor 75005701
TX-150 round buckets 75005702
TX-150 50mL conical buckets 75005703
TX-100S clinical swinging bucket rotor with sealed carriers 75005704
TX-100 clinical swinging bucket rotor with carriers 75005705
M10 microplate swinging bucket rotor 75005706
M10 buckets 75005723
M10 sealed buckets 75005721
MT-12 microtube swinging bucket rotor 75005600
HIGHConic III fixed angle rotor 75005709
CLINIConic fixed angle rotor 75003623
MicroClick 18 x 5 microtube rotor 75005765
MicroClick 24 x 2 microtube rotor 75005715
MicroClick 30 x 2 microtube rotor 75005719
Microliter 48 x 2 microtube rotor 75003602
8 x 8 PCR Strip rotor 75005720
8 x 50 mL Individually Sealed rotor 75003694
Hematocrit rotor* 75005733
* Look at the separate Thermo Scientific Hematocrit Rotor Instruction Manual for specific data and instructions.
1

Chapter 1 | TX-150
10 |Small Benchtop Rotors Thermo Scientific
TX-150
Items Supplied
Article No. Item Quantity
75005701 TX-150 Rotor 1
75003786 Bolt Grease 1
50139640 Instruction Manual 1
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Technical Data
TX-150 with round buckets
Megafuge 8R, Sorvall ST 8R and SL 8R Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 2.9 kg 2.9 kg 2.9 kg
Maximum Permissible Load 4 x 190 g 4 x 190 g 4 x 190 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 12968 12968 12968
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 51 mm 144 mm / 51 mm 144 mm / 51 mm
Angle 90° 90° 90°
Acceleration / Braking Time 20 s / 30 s 20 s / 30 s 20 s / 30 s
Maximum Speed at 4 °C 50 Hz: 4500 rpm
60 Hz: 4500 rpm 60 Hz: 4500 rpm 50 Hz: 4500 rpm 60 Hz: 4500 rpm
Sample Temperature at Max. Speed
(Ambient temperature of 23 °C, run time 90 minutes)
50 Hz: < 4 °C
60 Hz: < 4 °C 60 Hz: < 4 °C 50 Hz: < 4 °C 60 Hz: < 4 °C
Aerosol-tight Optional Optional Optional
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C

TX-150 | Chapter 1
Thermo Scientific Small Benchtop Rotors |11
Megafuge 8, Sorvall ST 8 and SL 8 Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 2.9 kg 2.9 kg 2.9 kg
Maximum Permissible Load 4 x 190 g 4 x 190 g 4 x 190 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 12968 12968 12968
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 51 mm 144 mm / 51 mm 144 mm / 51 mm
Angle 90° 90° 90°
Acceleration / Braking Time 12 s / 18 s 13 s / 19 s 13 s / 19 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 5 °C 5 °C 5 °C
Aerosol-tight Yes Yes Yes
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C

Chapter 1 | TX-150
12 |Small Benchtop Rotors Thermo Scientific
TX-150 with conical buckets
Megafuge 8R, Sorvall ST 8R and SL 8R Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 2.9 kg 2.9 kg 2.9 kg
Maximum Permissible Load 4 x 150 g 4 x 150 g 4 x 150 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 14532 14532 14532
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 45 mm 144 mm / 45 mm 144 mm / 45 mm
Angle 90° 90° 90°
Acceleration / Braking Time 20 s / 30 s 20 s / 30 s 20 s / 30 s
Maximum Speed at 4 °C 50 Hz: 4500 rpm
60 Hz: 4500 rpm 60 Hz: 4500 rpm 50 Hz: 4500 rpm 60 Hz: 4500 rpm
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes)
50 Hz: < 4 °C
60 Hz: < 4 °C 60 Hz: < 4 °C 50 Hz: < 4 °C 60 Hz: < 4 °C
Aerosol-tight Optional Optional Optional
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C

TX-150 | Chapter 1
Thermo Scientific Small Benchtop Rotors |13
Megafuge 8, Sorvall ST 8 and SL 8 Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 2.9 kg 2.9 kg 2.9 kg
Maximum Permissible Load 4 x 150 g 4 x 150 g 4 x 150 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 14532 14532 14532
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 45 mm 144 mm / 45 mm 144 mm / 45 mm
Angle 90° 90° 90°
Acceleration / Braking Time 12 s / 18 s 13 s / 19 s 13 s / 19 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 7 °C 7 °C 7 °C
Aerosol-tight No No No
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C

Chapter 1 | TX-150
14 |Small Benchtop Rotors Thermo Scientific
Accessories
Article No. Description
Rotor
Capacity
(places x
volume, mL)
Max Tube
Dimensions
(Ø x L, mm)
75005703 50 mL Conical Buckets (unsealed, no adapter needed) (set of 4) 8 x 50 29.5 x 120
75005702 Round Buckets (set of 4) 4 x 145 50 x 100
75005707 Click Seal Biocontainment Lids for Round Buckets (set of 4)
75005724 Replacement O-rings for Lids (set of 4)
Adapters for 50 mL Conical Buckets (sets of 2)
75005808 15 mL Conical Tube 8 x 15 17 x 123
Adapters for Round Buckets (sets of 4)
Direct fit 145 mL Bottle (75005734) 4 x 145 50 x 100
175005735 100 mL Round Bottom Open-Top Tube 4 x 100 45 x 117
275005736 50 mL Conical or Skirted Tube 4 x 50 29.5 x 120
375005744 30 mL Sterilin™ Universal Container 4 x 30 25 x 120
475005737 15 mL Conical Tube 8 x 15 17 x 122
475005737 11 mL IVF Tube 8 x 11 17 x 122
575003504 13 mL Urine Tube 16 x 13 17 x 110
575003504 12 mL Blood Collection Tube (Greiner™) 16 x 12 17 x 110
575003504 10 mL Blood Collection or 15 mL Corex™/Kimble™ Tube 16 x 15 17 x 110
675005739 5/7 mL Blood Collection Tube 24 x 5/7 13 x 110
775005740 3/5 mL Blood Collection Tube or Cryotube 28 x 3/5 13 x 110
875005743 1.5/2 mL Microtube (or Microtainer™ tube) 40 x 2 11 x 65
Rotor Packages
75005760 Cell Culture Package,
INCLUDES: TX-150 Rotor (75005701), Round Buckets (75005702),
Adapters for 50 mL Conical Tubes (75005736)
4 x 50 29.5 x 120
75005761 High Capacity Cell Culture Package,
INCLUDES: TX-150 Rotor (75005701), Conical Buckets (75005703),
Adapters for 15 mL Conical Tubes (75005808)
8 x 50 18 x 124
75005762 Clinical Rotor Package,
INCLUDES: TX-150 Rotor (75005701), Round Buckets (75005702),
ClickSeal Biocontainment Lids (75005707), Adapters for Blood
Collection Tubes: 5/7 mL (75005739) and 10 mL (75005738)
24 x 5/7 18 x 124
12345678

TX-150 | Chapter 1
Thermo Scientific Small Benchtop Rotors |15
Biocontainment Certificate

Chapter 1 | TX-100S
16 |Small Benchtop Rotors Thermo Scientific
TX-100S
Items Supplied
Article No. Item Quantity
75005704 TX-100S Rotor 1
75003786 Bolt Grease 1
50139640 Instruction Manual 1
20059135 Carriers 4
50110911 Metal Sleeves 8
50110924 Biocontainment Lids 8
20059364 O-Rings 8
76001803 Rubber Pads 8
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Technical Data
Megafuge 8R, Sorvall ST 8R and SL 8R Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 3.1 kg 3.1 kg 3.1 kg
Maximum Permissible Load 8 x 25 g 8 x 25 g 8 x 25 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 14813 14813 14813
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 45 mm 144 mm / 45 mm 144 mm / 45 mm
Angle 90° 90° 90°
Acceleration / Braking Time 20 s / 30 s 20 s / 30 s 20 s / 30 s
Maximum Speed at 4 °C 50 Hz: 4500 rpm
60 Hz: 4500 rpm 60 Hz: 4500 rpm 50 Hz: 4500 rpm 60 Hz: 4500 rpm
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes)
50 Hz: < 4 °C
60 Hz: < 4 °C 60 Hz: < 4 °C 50 Hz: < 4 °C 60 Hz: < 4 °C
Aerosol-tight Optional Optional Optional
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C

TX-100S | Chapter 1
Thermo Scientific Small Benchtop Rotors |17
Megafuge 8, Sorvall ST 8 and SL 8 Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 3.1 kg 3.1 kg 3.1 kg
Maximum Permissible Load 8 x 25 g 8 x 25 g 8 x 25 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 14258 14258 14258
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 44 mm 144 mm / 44 mm 144 mm / 44 mm
Angle 90° 90° 90°
Acceleration / Braking Time 12 s / 18 s 13 s / 19 s 13 s / 19 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 7 °C 7 °C 7 °C
Aerosol-tight Yes Yes Yes
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C
Accessories
Article No. Description
Rotor
Capacity
(places x
volume, mL)
Max Tube
Dimensions
(Ø x L, mm)
Adapters for TX-100S Clinical Rotor (each)
11172596 5/7 mL BD Hemogard™/BD Vacutainer™ Tube 16/8 x 5/7 13 x 110
11172595 5 mL BD Hemogard Tube 16/8 x 5 13 x 75
11172287 3 mL Blood Collection Tube 16/8 x 3 11 x 70
11172288 1.5/2 mL Microtube (or Microtainer™ tube) 16/8 x 1.5/2 10 x 41

Chapter 1 | TX-100S
18 |Small Benchtop Rotors Thermo Scientific
Biocontainment Certificate

TX-100 | Chapter 1
Thermo Scientific Small Benchtop Rotors |19
TX-100
Items Supplied
Article No. Item Quantity
75005705 TX-100 Rotor 1
75003786 Bolt Grease 1
50139640 Instruction Manual 1
20059134 Carriers 4
50110904 Metal Sleeves 16
76001803 Rubber Pads 16
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Technical Data
Megafuge 8R, Sorvall ST 8R and SL 8R Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 3.3 kg 3.3 kg 3.3 kg
Maximum Permissible Load 16 x 25 g 16 x 25 g 16 x 25 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 14258 14258 14258
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 46 mm 144 mm / 46 mm 144 mm / 46 mm
Angle 90° 90° 90°
Acceleration / Braking Time 20 s / 30 s 20 s / 30 s 20 s / 30 s
Maximum Speed at 4 °C 50 Hz: 4500 rpm
60 Hz: 4500 rpm 60 Hz: 4500 rpm 50 Hz: 4500 rpm 60 Hz: 4500 rpm
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes)
50 Hz: < 4 °C
60 Hz: < 4 °C 60 Hz: < 4 °C 50 Hz: < 4 °C 60 Hz: < 4 °C
Aerosol-tight No No No
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C

Chapter 1 | M10
20 |Small Benchtop Rotors Thermo Scientific
Megafuge 8, Sorvall ST 8 and SL 8 Centrifuges
Centrifuge Frequency 230 V 120 V 100 V
Weight (empty) 3.3 kg 3.3 kg 3.3 kg
Maximum Permissible Load 16 x 25 g 16 x 25 g 16 x 25 g
Maximum Speed nmax 4500 rpm 4500 rpm 4500 rpm
Maximum RCF-Value at nmax 3260 x g 3260 x g 3260 x g
K-Factor at nmax 14258 14258 14258
Max. Cycle Number 50000 50000 50000
Radius max. / min. 144 mm / 46 mm 144 mm / 46 mm 144 mm / 46 mm
Angle 90° 90° 90°
Acceleration / Braking Time 12 s / 18 s 13 s / 19 s 13 s / 19 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 7 °C 7 °C 7 °C
Aerosol-tight No No No
Permissible Temperature Range Autoclavable 121 °C 121 °C 121 °C
Accessories
Article No. Description
Rotor
Capacity
(places x
volume, mL)
Max Tube
Dimensions
(Ø x L, mm)
Adapters for TX-100 Rotor (each)
Direct fit 13 mL Urine Tube 16/8 x 13 17 x 110
11172596 5/7 mL BD Hemogard™/BD Vacutainer™ Tube 16/8 x 5/7 13 x 110
11172595 5 mL BD Hemogard Tube 16/8 x 5 13 x 75
11172287 3 mL Blood Collection Tube 16/8 x 3 11 x 70
11172288 1.5/2 mL Microtube (or Microtainer™ tube) 16/8 x 1.5/2 10 x 41