
Ballast-Check 2 User’s Manual
oIf LOW risk is displayed, the measurement is complete and you can see values for
that sample by pressing the key.
oIf the sample has a HIGH risk, you will be prompted to insert a 10 micron sample.
12. Attach a 10 micron filter to your 60cc syringe.
13. Remove the cuvette from your Ballast-Check 2 and discard the sample.
14. Using the remaining sample in your syringe, rinse the glass cuvette 3 times, then fill the
cuvette ¾ full with your 10 micron filtered sample.
15. Dry and clean all faces of the cuvette using Kim Wipes.
16. Insert the cuvette into your Ballast-Check 2 and close the lid.
17. Press READ.
18. Results will be displayed as HIGH or LOW risk. The measurement is complete.
Associated values can be viewed by pressing the key.
19. Remove the cuvette from the Ballast-Check 2 and discard the sample
20. Remove the 10 micron filter from your 60cc syringe and discard the sample remaining in
the syringe.
21. Rinse the syringe 3 times with DI.
22. Using the filter washing kit, back wash the 10 micron filter with a clean water source
such as deionized or distilled water to prepare it for reuse or storage. See Filter
Washing Procedure in Section 2.8 for more information on filter washing.
23. When finished viewing data, or if ready to run the next sample, press ESC to get to the
Home screen and repeat sampling procedure.
Note: It is recommended you repeat the above steps 3 times. If you see inconsistent
results, please refer to the Sample Analysis Guidelines in Section 3.
2.6 Data Displayed
Data can be viewed on the Ballast-Check 2’s display or downloaded to a computer for
viewing. To view data on the Ballast-Check 2’s display, from the HOME screen, use the
keys to scroll through sample numbers until you’ve reach the sample number you
wish to view. Then use the keys to scroll through logged data for that sample
number. You can view the date, time, risk, ship/tank values recorded and the
Abundance and Activity value saved for that sample. You can also view these
parameters using the keys right after a measurement has completed.
Values for Abundance and Activity are defined by the set fluorescence thresholds which
correlate to the discharge standard of less than 10 viable organisms of the 10-50 micron
size class in 1 milliliter of ballast water.
The Abundance value represents an enumeration of the algal cells in the sample and
addresses the “…less than 10 organisms…” requirement of the discharge standard. The
Ballast-Check 2 has a set threshold value of 10 cells per milliliter. This threshold is part
of the information used to determine whether samples are at risk for gross exceedance
of the discharge standard.
Addressing the discharge standard’s “…viable organisms…” is done by measuring the
photosynthetic efficiency (Activity) of algae in ballast water samples. The Activity is