Turner Designs C-FLUOR User manual

User’s Manual
April 8, 2019
P/N 998-2123
Revision 1.0
TURNER DESIGNS
1995 N. 1st Street
San Jose, CA 95112
Phone: [phone]
FAX: [phone]

Table of Contents
1.
Introduction
1.1
Description
4
2.
Inspection and Setup
2.1
Instrument Checklist
5
2.2
Configurations
5
2.3
Optional Accessories
5
2.4
Functional Test for C-FLUOR with connector
7
3.
Measurements with the C-FLUOR
3.1
Introduction
8
3.2
Determine a Different Offset
8
3.3
Determine a Different Calibration Coefficient
8
3.4
Performing C-FLUOR Measurements
9
4.
Maintenance and Warranty
4.1
Maintenance
10
4.1.1
Rinsing
10
4.1.2
Care for the bulkhead connector
10
4.1.3
Care for the Optics
10
4.2
Warranty Terms
10
4.3
Warranty Service
11
4.4
Out of Warranty Service
11
Appendices
A
Specifications
13
B
Recommended Lab Measurement Practices
14
C
Wiring Guide
15
D
Pigtail Cable and Connector Information
16
E
Linear Range, Quenching and Temperature Considerations
17
F
Functional Test for C-FLUOR with No Connector
18
G
Using the C-FLUOR Submersible Probe with the DataBank
19

WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) DIRECTIVE
Turner Designs is in the business of designing and selling products that benefit the
well-being of our environment. Accordingly, we are concerned with preserving the
surroundings wherever our instruments are used and happy to work with customers by
complying with the WEEE Directive to reduce the environmental impact resulting from
the use of our products.
WEEE Return Process:
To arrange the return of an end-of-life product, proceed as follows:
If you purchased your instrument through a Turner Designs Distributor please
contact your local representative. They will instruct you where to return the end-of-
life product.
If you purchased your instrument directly from Turner Designs please contact
Turner Designs Customer Service
By Phone: [phone] or Toll Free: [phone]
By Email: Customer Service at [e-mail]
Turner Designs will provide a WEEE RMA Number, a Shipping Account Number,
and a Ship to Address. Package and ship the product back to Turner Designs.
The product will be dealt with per Turner Designs’ end-of-life recycling program in an
environmentally friendly way.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 4
1. Introduction
1.1 Description
The Turner Designs C-FLUOR Submersible Probe is an accurate single-channel
detector that can be used for many different applications. It is designed for
integration into multi-parameter systems from which it receives power and
delivers a voltage output proportional to the concentration of the fluorophore,
particle, or compound of interest.
The C-FLUOR is factory calibrated. A probe-specific calibration certificate is
included with each C-FLUOR probe. The certificate contains calibration values
that are used to convert voltage to concentration estimates.
C-FLUOR is manufactured with a Titanium housing and connector, ideal for
long-term deployments and able to withstand most corrosive environments. It is
available without a connector for integration flexibility; see Section 2.2 for part
numbers.
NOTE: C-FLUOR cannot be used with the C6 Multi-Sensor Platform.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 5
2. Inspection and Setup
2.1 Instrument Checklist
The C-FLUOR Submersible Probe shipment package consists of:
C-FLUOR Submersible Probe:
Configured and factory calibrated for the specified fluorophore or material as
noted by the Identification Letter stamped on the connector:
“C” = Chlorophyll
“R” = Rhodamine
“F” = Fluorescein
“P” = Phycocyanin
“E” = Phycoerythrin
“U” = CDOM / FDOM
“O” = Crude Oil
“B” = Optical Brighteners
“T” = Turbidity
“D” = Red Excitation
Calibration Certificate
Quick Start Guide
USB Flash Drive if accessories are ordered.
2.2 Configurations:
C-FLUOR P/N: 2120-000-“Identification Letter”
No Connector C-FLUOR P/N: 2120-000-“Identification Letter”-NC
2.3 Optional Accessories include:
Pigtail Cables with Locking Sleeve - see Appendix D for more information
0.6 meter Pigtail Cable with Locking Sleeve P/N 2100-750
5 meter Pigtail Cable with Locking Sleeve P/N 2100-755
10 meter Pigtail Cable with Locking Sleeve P/N 2100-751
25 meter Pigtail Cable with Locking Sleeve P/N 2100-752
50 meter Pigtail Cable with Locking Sleeve P/N 2100-753
Note: Pigtail Cables should NOT be used to tow instruments.
DataBank Handheld Data Logger P/N: 2900-199 - see Appendix G
Flow Cap P/N: 2100-600 - see Accessory Instructions on USB Flash Drive
Shade Cap P/N: 2100-701 - see Accessory Instructions on USB Flash Drive
Note: We recommend use of the shade cap as it provides a fixed distance
for sample measurement and minimizes effects from ambient light.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 6
Explorer P/N: 2100-485 –see Accessory Instructions on USB Flash Drive
Solid Secondary Standard (SSS) for in vivo Chlorophyll, Phycocyanin,
Phycoerythrin, Rhodamine, Fluorescein P/N: 2100-900 - see Accessory
Instructions on USB Flash Drive
Note: The SSS can be used for both C-FLUOR and Cyclops for the
applications specified above.
Solid Secondary Standard (SSS) for UV - CDOM / FDOM, Optical
Brighteners, and Crude Oil P/N: 2100-904 - see Accessory Instructions on
USB Flash Drive
Note: The UV SSS can be used for both C-FLUOR and Cyclops for the
applications specified above, as well as Cyclops Refined Fuels and
Tryptophan sensors.
Flow Cap
Shade Cap
Solid Secondary Standard

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 7
2.4 Functional Test for C-FLUOR with connector
To perform a functional check on a C-FLUOR Probe, connect the Pigtail’s
colored wires to the power supply and multi-meter as shown in Figure 1 below.
Additional Equipment required for functional tests:
DC Power Supply, 3 - 15 VDC, >100 mA
Multi-meter to read 0 –5 VDC
Note: Supply voltages greater than 15 VDC will damage the probe.
With the C-FLUOR connected as shown in Figure 1 answer questions 1-3 by
making the following functional tests:
1. Is the LED on?
Hold a piece of white paper about ½ an inch in front of the optical head to
ensure the LED is ON.
Note: This test does not work for Turbidity probes because they use
infrared which is not visible.
2. Is there voltage output?
The multi-meter should be reading some voltage >0 VDC
3. Does the voltage output change?
Move the light source closer to the piece of paper and check if the voltage
output increases
DC Power Supply
3.52
Multimeter
10 VDC
+-
Figure 1
12.00 .01
+ -
PSU Positive Connection
(Red) Supply Ground 0 VDC (Black)
Signal Output
“+” (White)
Analog Ground
“-” (Green)
Pigtail Cable
3
6
4
2
1
55
2
4
4
6
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3
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2
1
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Pigtail cable holes
33
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55 5
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6
Pigtail pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
33
44
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1
55
6
6
33
44
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1
55
6
6

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 8
3. Measurements with the C-FLUOR
3.1 Introduction
The following information will describe how to:
oDetermine a Different Offset –ONLY REQUIRED IF CHANGING FACTORY
OFFSET
oDetermine a Different Calibration Coefficient–ONLY REQUIRED IF
CHANGING FACTORY CALIBRATION
oPerform C-FLUOR Measurements
Note: To make accurate and repeatable measurements it is important to
keep the probe clean; see Section 4.1 for information regarding
maintenance.
C-FLUOR Probes output a voltage signal that is proportional to the fluorophore
of interest. Every C-FLUOR is factory calibrated and delivered with a certificate
that contains factory calibration values for that specific probe; the certificate also
contains an equation that can be used with the calibration values to convert C-
FLUOR’s voltage signal to concentration estimates for the specified fluorophore
of interest.
For increased accuracy when estimating fluorophore concentrations you can
create user-defined calibration values. Sections 3.2 and 3.2 explain how to
derive your own calibration values for your environment or fluorophore of
interest. If you want to use the factory calibration, skip to Section 3.4.
3.2 Determine a Different Offset
C-FLUOR’s Factory Offset is measured using ultra-pure water. When working in
an environment where background fluorescence values are greater than the
factory measured Offset, you may want to consider using an Offset that is more
suited for your water type. To determine an Offset for your water type:
1) Collect a sample of water that represents the water type you will be
measuring.
2) Remove the fluorophore of interest from that water sample. For example, for
in vivo chlorophyll you would filter the water sample through a GF/F or like
filter to remove all algal cells from the sample.
3) Once the fluorophore of interest has been removed from the water sample,
measure that sample using the C-FLUOR and record the voltage response.
4) Your Offset will be equal to that voltage response so all voltage
measurements for that water type should have this Offset subtracted.
3.3 Determine a Different Calibration Coefficient
The Calibration Coefficient stated on the calibration certificate is a correlation
between a standard of known concentration and the Offset-corrected response
for that standard. To create your own calibration coefficient:
1) Determine an Offset for your water type - see Section 3.2.
2) Obtain a standard which,
a. Has a known concentration.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 9
b. Falls within the measuring range of the C-FLUOR.
3) Measure the response of that standard.
4) Subtract the Offset from that measured response.
5) Divide the concentration of the standard by its Offset-corrected response.
6) Your result is a calibration coefficient that can be used to convert Offset-
corrected voltages to direct concentrations.
3.4 Performing C-FLUOR Measurements
C-FLUOR Probes output a 0-5 Volt analog signal proportional to the
fluorescence detected from the fluorophore of interest. Use the wiring diagram
from Section 2.4 to connect the C-FLUOR to a power supply and a multi-meter
that will read voltages from the probe. Submerge the optical head, or entire
probe, into the water sample; the voltage displayed indicates the relative amount
of fluorophore in your sample.
If reading samples in a container, see Appendix B for recommended
measurement practices.
C-FLUOR Probes can be integrated into any third party system that is capable
of supplying 3 –15 VDC and accepting a 0-5 Volt analog signal.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 10
4. Maintenance and Warranty
4.1 Maintenance
4.1.1 Rinsing
The C-FLUOR should be rinsed or soaked in fresh water following each
deployment until it is completely clean again.
4.1.2 Care for the bulkhead connector
A light coat of Silicone spray should be used on the rubber of the male
pins of the bulkhead to aid in sealing. The manufacturer recommends
3M™ Silicone Lubricant Spray or Loctite 8021 spray.
Note: You should avoid using silicone grease. Do NOT use WD-40,
it will destroy the connectors.
4.1.3 Care for the optics
The optical window should be visually inspected after each deployment
following a soaking in fresh water. If cleaning is needed, use optical
tissue to clean the window with soapy water.
Note: The C-FLUOR should NOT come in contact with any organic
solvents (i.e. acetone, methanol) or strong acids and bases.
4.2 Warranty Terms
Turner Designs warrants the C-FLUOR and accessories to be free from defects
in materials and workmanship under normal use and service for a period of 12
months from the date of shipment from Turner Designs with the following
restrictions:
Turner Designs is not responsible for replacing parts damaged by accident
or neglect. Your instrument must be installed according to instructions in the
User’s Manual. Damage from corrosion is not covered. Damage caused by
customer modification of the instrument is not covered.
This warranty covers only Turner Designs products and is not extended to
equipment used with our products. We are not responsible for incidental or
consequential damages, except in those states where this limitation is not
allowed. This warranty gives you specific legal rights and you may have
other rights which vary from state to state.
Damage incurred in shipping is not covered.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 11
4.3 Warranty Service
To obtain service during the warranty period, the owner shall take the following
steps:
1. Write, email or call Turner Designs Technical Support and describe as
precisely as possible the nature of the problem.
Phone: [phone]
Email: [e-mail]
2. Carry out any adjustments or tests as suggested by Technical Support.
3. If proper performance is not obtained you will be issued a Return Materials
Authorization number (RMA) to reference. Package the unit, write the RMA
number on the outside of the shipping carton, and ship the instrument,
prepaid, to Turner Designs. If the failure is covered under the warranty
terms the instrument will be repaired and returned free of charge, for all
customers in the contiguous continental United States.
For customers outside of the contiguous continental United States who
purchased equipment from one of our authorized distributors, contact the
distributor. If you purchased directly, contact us. We will repair the
instrument at no charge. Customer pays for shipping, duties, and
documentation to Turner Designs. Turner Designs pays for return shipment
(custom duties, taxes and fees are the responsibility of the customer).
4.4 Out-of-Warranty Service
Follow steps for Warranty Service as listed above. If Technical Support can
assist you by phone or correspondence, we will be glad to, at no charge. Repair
service will be billed on a fixed price basis, plus any applicable duties and/or
taxes. Shipment to Turner Designs should be prepaid. Your bill will include
return shipment freight charges.
Address for Shipment:
Turner Designs, Inc.
1995 N. 1st Street
San Jose, CA 95112

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 12

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 13
Appendix A: Specifications
Parameter
Specification
Full Range Linearity
0.99 R2
Power Draw @ 12V
CHL, RWT, PC, PE, F, CDOM/fDOM, Oil, OB = 12mA
Turbidity = 10mA
Input Voltage
3 –15 VDC
Signal Output
0 –5 VDC Analog
Temperature Range
Ambient: 0 to 50 C
Water Temp: -2 to +50 C
Light Source
Light Emitting Diode
Excitation Wavelength
Visible –CHL, RWT, PC, PE, F
UV –CDOM/fDOM, Oil, OB
IR –Turbidity
Detector
Photodiode
Detection Wavelengths
365 –850 nm
T99
<0.6 seconds
Housing Material
Titanium
Dimensions
C-FLUOR
L: 5.2 in., 13.24 cm
D: 0.875 in., 2.23 cm
No Connector C-FLUOR
L: 3.8 in., 9.68 cm
D: 0.875 in., 2.23 cm
Depth Rating
2,000 meters
Weight
3.1 oz; 89 gm

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 14
Appendix B: Recommended Lab Measurement Practices
Recommended Lab Practices for Measurements
The following steps will improve the accuracy and repeatability of your measurements,
especially at low concentration levels:
1. Use a non-fluorescent container for your water samples. Note: Plastic may
fluoresce and interfere with the sample’s fluorescence.
2. If using a glass container, place the container on a non-reflective black surface.
3. Ensure that the probe is more than 3 inches above the bottom of the container.
4. Ensure that the probe is in the center of the container and has more than 2 inches
clearance between the cirumference of the probe and the inside surface of the
beaker.
>3 inches
Dark/Black
Non-Reflective
Surface
>2 inches all
round
Calibrated
Sensor
No Air Bubbles
On Optical
Surface
Glass Beaker

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 15
Appendix C: Wiring Guide
C-FLUOR
Pins/Wires
Pin
Number
Function
Connection
Red
1
Supply Voltage
3 –15 VDC
PSU –Positive Connection
Black
2
Supply Ground, 0VDC
PSU –Ground Connection
Orange
3
Signal Out to data logger “+”
0 –5VDC
Multimeter Positive
Connection
Green
4
Analog Ground “-”,
0 VDC
Multimeter Negative
Connection
Note: Pins 5 and 6 are not used.
Pigtail Connector
and Wire Color
3
6
4
2
1
5
5
2
4
4
6
1
3
C-FLUOR Pins
and Wire Color
3
4
2
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6
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6

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 16
Appendix D: Pigtail Cable and Connector Information
Dimension details of 24” length cable with 20 gauge colored lead wire, connects to 6 pin
male connector. (Cable manufacturer/Part No: IMPULSE/MCIL-6-FS)
Female locking sleeve,
(Impulse P/No. MCDLS-F)
C-FLUOR Pins
and Wire Color
3
4
2
1
5
6
6
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
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55
6
6
Pigtail Connector
and Wire Color
3
6
4
2
1
5
5
2
4
4
6
1
3
3
6
4
2
1
5
5
2
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1
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5
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1
3
A maximum cable length up to 100 meters canbe connected to the Cyclops if the
following conditions are met:
1) The cable is shielded and contains 20gauge conductor size orgreater
(i.e.Beldon No. 8426 cable).
2) The 0-5 volt Analog output isconnected to a device (i.e.Data Logger) with
an input impedance of 1 MegOhm or greater.
3) The supply Voltage to the Cyclops is between 5 and 15 volts.
A maximum cable length up to 100 meters canbe connected to the C-FLUOR if the
following conditions are met:
1) The cable is shielded and contains 20gauge conductor size orgreater
(i.e.Beldon No. 8426 cable).
2) The 0-5 volt Analog output isconnected to a device (i.e.Data Logger) with
an input impedance of 1 MegOhm or greater.
3) The supply Voltage to the C-FLUOR isbetween 5and 15 volts.

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 17
Appendix E: Linear Range, Quenching and Temperature Considerations
The linear range is the concentration range in which the fluorometer’s output is directly
proportional to the concentration of the signal. The linear range begins with the
smallest detectable concentration and spans to an upper limit (concentration) that is
dependent upon the properties of the material, filters used, and path length.
A non-linear relationship is seen at very high concentrations where the signal does not
increase at a constant rate in comparison to the change in concentration - see figure
below. At even higher concentrations, the signal will decrease even though the
sample concentrations are continuing to increase. This effect is known as “signal
quenching”.
Linearity can be checked by diluting a sample 1:1 or some other convenient ratio. If
the sample is still in the linear range, the reading will decrease in direct proportion to
the dilution. If the reading does not decrease in direct proportion to the dilution, or if
the reading increases, the sample is beyond the linear range.
Temperature Considerations
Fluorescence is temperature sensitive. As the temperature of the sample increases,
the fluorescence decreases. For greatest accuracy, record the sample temperature
and correct the probe output for changes in temperature.
For further information on how temperature, light, water quality and the physiological
state of the algal cells can all affect measurements. Please refer to the Frequently
Asked Questions in Technical Support on Turner Designs’ website for more
information about temperature compensation.
Graph showing Linear and
Quenching Regions of the
sample’s response
Sample Concentration
Fluorometer
Reading
Fluorometer Response Curve
Sample
Quenching Region
Sample
Linear Region
Graph showing Linear and
Quenching Regions of the
sample’s response
Sample Concentration
Fluorometer
Reading
Fluorometer Response Curve
Sample
Quenching Region
Sample
Linear Region
Sample Concentration
Fluorometer
Reading
Fluorometer Response Curve
Sample
Quenching Region
Sample
Linear Region

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 18
DC Power Supply
3.52
Multimeter
10 VDC
+-
Figure 2
12.00 .01
+ -
PSU Positive Connection
(Red) Supply Ground 0 VDC (Black)
Signal Output
“+” (White)
Analog Ground
“-” (Green)
Note: Yellow and Blue wires are not used.
Appendix F: Functional Test for C-FLUOR with No Connector
To perform a functional check on the C-FLUOR, connect the C-FLUOR’s colored
wires to the power supply and multi-meter as shown in Figure 2 below.
No Connector C-FLUOR (P/N: 2120-000-“Identification Letter”-NC)
Additional Equipment required for functional tests:
DC Power Supply, 3 - 15 VDC, >100 mA
Multi-meter to read 0 –5 VDC
Note: Supply voltages greater than 15 VDC will damage the probe.
With the C-FLUOR connected as shown in Figure 1 answer questions 1-3 by
making the following functional tests:
4. Is the LED on?
Hold a piece of white paper about ½ an inch in front of the optical head to
ensure the LED is ON.
Note: This test does not work for Turbidity probes because they use
infrared which is not visible.
5. Is there voltage output?
The multi-meter should be reading some voltage >0 VDC
6. Does the voltage output change?
Move the light source closer to the piece of paper and check if the voltage
output increases

C-FLUOR Submersible Probe
998-2123 Rev. 1.0 Page 19
Appendix G: Using the C-FLUOR Submersible Probe with the DataBank
C-FLUOR Probes are analog output devices that produce a 0 –5 Volt signal
proportional to the fluorophore being measured. Turner Designs offers the DataBank,
a universal handheld meter, datalogger, and power supply that can be used to
maximize performance of C-FLUOR Probes with functions such as:
User defined calibrations
Integrated Internal GPS
Large internal memory
Interval logging
The DataBank comes with intuitive GUI software that allows users to easily calibrate,
set up logging, download data, and define parameters and values necessary to help
configure the C-FLUOR for a specific application or study. Available options include
travel case, and car charger.
DataBank uses for different sampling protocols:
Multiple Site Measurements –measure fluorescence at different locations within your
water system or across many systems; GPS provides latitude/longitude data per
location
Profiling –purchase extended cables to allow for vertical profiling
Monitoring –deploy the C-FLUOR to a fixed location or depth and monitor the signal
over time; set up logging to capture a signal within a specific time frame; download
data while the probe is deployed and continuously measuring; set up digital data
output via HyperTerminal
For any application or sampling protocol, the DataBank facilitates and maximizes
performance of all C-FLUOR Probes allowing versatility and flexibility in calibration,
setup, and measurement.