Turner Designs Ballast-Check 2 User manual

Ballast-CheckTM2
Handheld PAM Fluorometer
December 5, 2014
P/N: S-0156
Revision DRAFT
TURNER DESIGNS
845 W. Maude Ave.
Sunnyvale, CA 94085
Phone: [phone]
FAX: [phone]

Ballast-Check 2 User’s Manual 2
Table of Contents
1
Introduction
1.1 Description 4
1.2 PAM Fluorometry 4
1.3 Quick View Diagrams 4
1.4 Inspection 5
1.5 General Information, Precautions & Cleaning 5
2
Instrument Operation and Calibration
2.1 Button Identification and Function 6
2.2 Instrument Power Up 7
2.3 Calibration Verification 8
2.4 Filtering Samples 8
2.5 Measuring a Sample 9
2.6 Data Downloading 10
3
Sample Analysis Guidelines
12
4
Measurement Parameters
4.1 Corrected Fluorescence (CFl) 13
4.2 Photosynthetic Efficiency (Activity) 13
4.3 Calculations 13
5
Warranty
5.1 Terms 14
5.2 Warranty Service 14
5.3 Out of Warranty Service 14
Appendixes
A Specifications 16

Ballast-Check 2 User’s Manual 3
WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) DIRECTIVE
Turner Designs is in the business of designing and selling products that benefit the well-being of
our environment. Accordingly, we are concerned with preserving the surroundings wherever
our instruments are used and happy to work with customers by complying with the WEEE
Directive to reduce the environmental impact resulting from the use of our products.
WEEE Return Process:
To arrange the return of an end-of-life product, proceed as follows:
If you purchased your instrument through a Turner Designs Distributor please contact your
local representative. They will instruct you where to return the end-of-life product.
If you purchased your instrument directly from Turner Designs please contact Turner
Designs Customer Service
By Phone: [phone] or Toll Free: [phone]
By Email: Customer Service at [email protected]
Turner Designs will provide a WEEE RMA Number, a Shipping Account Number, and a Ship
to Address. Package and ship the product back to Turner Designs.
The product will be dealt with per Turner Designs’ end-of-life recycling program in an
environmentally friendly way.

Ballast-Check 2 User’s Manual 4
1 Introduction
1.1 Description
The Ballast-Check 2 is configured to provide users with a rapid estimation of algal
abundance (Corrected Fluorescence or CFl) and photosynthetic efficiency (Activity) of algae
in water. The CFl (see section 4.1) value represents varying algal abundances used for
determining gross exceedance of algae in ballast water samples around the set threshold
level. Activity estimates are logged as a ratio (Fv/Fm) on a scale from 0.00 to 1.00 (see
section 4.2).
Abundance reporting is based on a fluorescence threshold:
Low: Corrected Fluorescence < 100
High: Corrected Fluorescence ≥ 100
Activity reporting is based on a yield threshold:
Low: Activity < 0.3
High: Activity ≥ 0.3
1.2 PAM Fluorometry
The Ballast-Check 2 is a Pulse Amplitude Modulated (PAM) Fluorometer. Pulse amplitude
modulation is a process that uses varying light intensities delivered in a series of pulses for
looking at fluorescence quenching to determine photosynthetic efficiency of algae or plant
material. There are two measurement modes, single and multiple turnover. The Ballast-
Check 2 is a multiple turnover PAM fluorometer in that one saturating pulse of light is
delivered to the sample to quickly reach the maximum fluorescence value with the interest of
only looking at how efficient algae are at photosynthesizing, essentially determining the
activity of the organisms. High degree of activity means a high survival rate and low activity
means the algae aren’t capable of efficiently photosynthesizing and have a low survival rate.
1.3 Quick View Diagrams
The Ballast-Check 2 uses four standard AAA user-replaceable batteries.

Ballast-Check 2 User’s Manual 5
1.4 Inspection
Upon receiving your instrument, please inspect everything carefully and make sure all
accessories are included. The Ballast-Check 2 package includes:
•The Ballast-Check 2
•4 - AAA batteries
•Solid Secondary Standard
•Two Glass 10x10mm Cuvettes
•One 60cc plastic syringe
•Five 50µfilter capsules
•Five 10µfilter capsules
•Five 0.2µfilter capsules
•Carrying Case
•USB Flash Drive with User’s Manual and Basic Instructions
1.5 General Information, Precautions and Cleaning
•Use caution around solvents, they may degrade the plastic case of the Ballast-Check 2.
•If a sample is accidentally spilled inside the sample compartment, invert the Ballast-
Check 2 to drain out the excess liquid. Then wipe the inside area dry with a clean soft
towel or tissue.
•If extra cleaning is needed, use a mild detergent to dampen the towel for cleaning.
•Although the Ballast-Check 2 floats, do not submerge it in water.
•Do not expose the Ballast-Check 2 to temperatures outside the specified range of
5 to 40 °C or damage may occur to the unit that will not be covered under warranty.

Ballast-Check 2 User’s Manual 6
2. Instrument Operation and Calibration
2.1 Button Identification and Function
SHIP – Displays the ship number as a 10-digit alphanumeric value always beginning with the
IMO prefix. Only the 7 digits following the IMO prefix can be adjusted to set the ship number.
After setting the ship number ENTER is pressed to save the value. If ESC is pressed at any
time, the screen is exited and nothing is saved.
Saved values are appended to a data point after a sample is analyzed. The sample number will
count sequentially, beginning with “Sample 1” for each set SHIP number. For example, if the
SHIP number is changed, the sample count will reset to start with “Sample 1” for that newly set
SHIP number.
TANK – Displays the tank number as a configurable 10-digit alphanumeric value. All characters
in the alphanumeric value can be adjusted to set a desired TANK number. After setting the
TANK number ENTER is pressed to save the value. If ESC is pressed at any time, the screen
is exited and nothing is saved.
Saved values are appended to a data point after a sample is analyzed. The sample number will
count sequentially, beginning with “Sample 1” for each set TANK number. For example, if the
TANK value is changed, the sample count will reset to start with “Sample 1” for that new set of
TANK values.
READ – Begins the sample analysis procedure detailed in section 2.5, only if SHIP and TANK
values are saved. If SHIP and TANK values are not saved, pressing READ will display the
message “First Set SHIP and TANK Values” and you will not be allowed to continue with
measuring a sample.
ON/OFF – Turns the instrument ON or OFF.
ENTER – This button is used to save set values when setting SHIP or TANK values and allows
users to proceed to the next screen/menu.

Ballast-Check 2 User’s Manual 7
DATA – When the DATA button is pressed users can choose between either sending/clearing
data or setting date/time. Use the up/down arrow keys to choose between the two options; a
marker will indicate the selection made.
If SEND/CLEAR DATA is selected, press ENTER and a submenu will display. Users can choose, using
up/down arrow keys, to either send data to a connected computer (see section 2.6) or clear logged data;
a marker will indicate the selection made.
oIf SEND DATA is selected and ENTER is pressed, the instrument will send all logged
data out as an ASCII file (see section 2.6).
oIf CLEAR DATA is selected, the following message will display; ENTER TO
CONFIRM CLEAR DATA; when ENTER is pressed all logged data will be deleted.
If SET DATE/TIME is selected, press ENTER and date and time will display on the screen as
(MM/DD/YYYY) and (hh:mm). Use the up/down arrow keys to select either parameter to set; a
marker will indicate selection.
oIf MM/DD/YYYY is selected, press ENTER and you will be allowed to adjust the
month, day, and year using the up/down/left/right arrow keys. Press ENTER to save
the set date. If ESC is pressed at any time, the screen is exited and nothing is
saved. Date is logged per sample.
oIf hh:mm is selected, press ENTER and you will be allowed to adjust the hour and
minutes using the up/down/left/right arrow keys. Press ENTER to save the set time.
If ESC is pressed at any time, the screen is exited and nothing is saved. Time is
logged per sample.
ESC – Exits to the previous screen or menu
CHK STD – Is used to determine instrument performance. PASS or FAIL will display after
measuring the solid standard using the CHK STD button indicating whether the Ballast-Check 2
can continue to be used for sample analysis or should be returned to the factory.

Ballast-Check 2 User’s Manual 8
2.2 Instrument Power Up
Press the <ON/OFF> button to power on the Ballast-Check 2. After a 5 second warm up,
the Ballast-Check 2 is ready for operation. Pressing the <ON/OFF> button again will turn
the unit off or if left idle for 3 minutes the unit will automatically turn off to save battery
power.
HOME SCREEN
The home screen will display the current date, time, Ballast-Check 2 firmware version, and
remaining battery power as a percentage. These parameters will be displayed after
powering the instrument on and warm up has completed as well as every time ESC button is
pressed to exit to the home screen.
If the batteries have low power, the following warning message will display – “Battery Low
Power! Replace Battery!”. You will not be able to make any measurements until batteries
are replaced.
2.3 Calibration Verification
The Ballast-Check 2 is factory calibrated and should remain calibrated for many years.
Calibration can be verified using the Solid Secondary Standard (SSS) included with the
instrument. To check instrument calibration:
1 Press the <ON/OFF> button to turn the Ballast-Check 2 on
2 Insert the SSS after warm up has completed and close the sample compartment lid
3 Press the CHK STD button
a. If the calibration is valid, the Ballast-Check 2 will display PASS
b. If the Ballast-Check 2 is out of calibration and needs to be sent back to the
manufacturer, FAIL will display
2.4 Filtering Samples
The Ballast-Check 2 comes with 3 types of filters that are used to analyze a sample: 0.2, 10,
and 50 micron sized filters. These filters are used to fractionate samples to measure
different sized algae. Each fractionated sample will be measured for fluorescence and the
corrected fluorescence value calculated at the end of the analysis will represent the 10-50
micron sized algal cells. The procedure in section 2.5 outlines how and when to use the
filters provided to perform the analysis.
01/06/2000 11:30
FW0.01 Batt: 20%

Ballast-Check 2 User’s Manual 9
2.5 Measuring a Sample
The following materials are required for measuring a sample:
-60 cc plastic syringe with male luer lock end
-50 micron mesh filter capsule
-10 micron mesh filter capsule
-0.2 micron mesh filter capsule
-Glass Cuvette
-Ballast-Check 2
-Solid Secondary Standard
-Wipes for wiping the outside of the glass cuvette
The following procedure is used to measure a sample:
1. Turn the Ballast-Check 2 ON.
2. Set the SHIP Number and TANK Number.
3. Using the 60 cc syringe, collect 60 ml of sample by aspirating from the source water.
4. Attach a 50 micron filter to the end of the plastic syringe.
5. Obtain a glass cuvette and filter some of the sample through the 50 micron filter into the
glass cuvette; rinse the cuvette 2-3 times with the 50 micron filtered sample before filling
the glass cuvette with the filtrate.
6. Wipe the outside of the glass cuvette, insert the cuvette into the Ballast-Check 2 and
close the sample compartment’s lid.
7. Press <READ> to measure the 50 micron filtered sample.
8. Remove the glass cuvette and discard the sample.
9. Remove the 50 micron filter from the syringe and attach a 10 micron filter to the end of
the 60cc syringe.
10. Filter some of the remaining sample in the syringe through the 10 micron filter into the
glass cuvette; rinse the cuvette 2-3 times with the 10 micron filtered sample before filling
the glass cuvette with the filtrate.
11. Wipe the outside of the glass cuvette, insert the cuvette into the Ballast-Check 2 and
close the sample compartment’s lid.
12. Press <READ> to measure the 10 micron filtered sample.
13. Remove the glass cuvette and discard the sample.
14. Remove the 10 micron filter from the syringe and attach a 0.2 micron filter to the end of
the 60cc syringe.
15. Filter some of the remaining sample in the syringe through the 0.2 micron filter into the
glass cuvette; rinse the cuvette 2-3 times with the 0.2 micron filtered sample before filling
the glass cuvette with the filtrate.
16. Wipe the outside of the glass cuvette, insert the cuvette into the Ballast-Check 2 and
close the sample compartment’s lid.
17. Press <READ> to measure the 0.2 micron filtered sample.

Ballast-Check 2 User’s Manual 10
18. After the final sample is measured, the screen will display results as:
Abund:LOW or HIGH and the associated fluorescence value as CFl
Activity:LOW or HIGH
All values in Figure 1 will be logged internally and can be downloaded at a later time.
19. Remove the glass cuvette from the Ballast-Check 2, discard the sample, and rinse the
glass cuvette with pure water.
20. Rinse the syringe with pure water.
Values for Abundance and Activity are defined by the set fluorescence threshold which
correlates to the IMO D2 discharge standard of less than 10 living organisms of the 10-50
micron size class in 1 milliliter of ballast water.
The Abundance reporting is based on the numerical part of the discharge standard (10
organisms of the 10-50 micron size class per milliliter) and termed Corrected Fluorescence
(CFl). If the CFl measured is greater than the set fluorescence threshold, then HIGH and
the associated fluorescence value are displayed indicating a gross exceedance of algal
abundance. If the CFl measured is less than the set threshold, then LOW and the
associated fluorescence value are displayed indicating a very low abundance of algae.
The Activity reporting of either LOW or HIGH is based on the activity part of the discharge
standard (living or active organisms) and is measured as photosynthetic efficiency of the
algae. If the calculated Activity is greater than 0.3, then HIGH is displayed indicating a high
degree of algal activity (i.e. live algae). If the calculated Activity is less than 0.3 then LOW is
displayed, indicating low algal activity or algae that have little activity and very low survival
rate.
The following parameters are recorded for each single measurement and can be
downloaded to a computer after they’ve been logged:
Date (MM/DD/YYYY)
Time (hh:mm)
Ship Number (IMO#######)
Tank Number (XXXXXXXXXX)
Sample Number (Sample XX)
Abundance - Corrected Fluorescence (CFl = XXXX.XX)
Activity - Photosynthetic Efficiency (Activity = X.XX)
Figure 1
2.6 Data Downloading
The Ballast-Check 2 can log a total of 1000 data points that can be downloaded directly to
your PC or computer using the provided serial cable. A terminal program (e.g.
Hyperterminal, Terra Term, MotoCross) is required for downloading these stored ASCII data
from the instrument. The following settings are required for connecting the Ballast-Check 2
with your terminal program:
Baud Rate: 57600