Turner Designs 8000 Manual

IOP 004C.12
Effective Date: 1/06/2017
Replaces IOP 004C.11
Page 1 of 3
U.S. Fish and Wildlife Service
Marquette Biological Station
3090 Wright Street
Marquette, Michigan 49855
U.S.A.
and
U.S. Fish and Wildlife Service
Ludington Biological Station
5050 Commerce Drive
Ludington, Michigan 49431
U.S.A.
and
Department of Fisheries and Oceans
Sea Lamprey Control Centre
1219 Queen Street East
Sault Ste. Marie, Ontario P6A 2E5
Canada
INSTRUMENT OPERATING PROCEDURE
INSTRUMENT:
Fluorometer
MODEL:
8000
MANUFACTURER:
Turner Designs
PRECAUTIONS:
POTENTIAL INTERFERENCES
Do not use glass or quartz cuvettes.
Use only lens cleaning tissues or cloth on plastic cuvettes.
Use a clean cuvette for all readings. The AquafluorTM is sensitive; sample residue may cause errors.
Fluorescence is temperature sensitive; read the blank, standard, and all samples at the same temperature.
High background or blank values may reduce sensitivity. Do not use this instrument for dye dilution
studies.
SAFETY

IOP 004C.12
Effective Date: 1/06/2017
Page 2 of 3
No special safety precautions
PROCEDURES:
I. Preparation of the AquafluorTM Fluorometer for use.
A. Turn on the instrument by pressing ON/OFF button. The instrument will countdown
from five after which it is ready to use.
B. If RHOD doesn’t appear in lower left of screen, press the A/B button. This toggles
between the Rhodamine and Turbidity channels.
II. Calibration of the fluorometer.
A. With a known standard.
1. Press the STD VAL button.
2. Use the up and down arrow buttons to adjust the readout to the standard value.
Holding the button down activates scrolling. When done, press ENT or ESC to
return to home screen.
3. Press the CAL button then the ENT button.
4. Insert a blank, close cover, and press ENT. Be sure the cuvette is at least half
full (>2 mL of sample), is free of bubbles, and is clean and dry. Wait until
prompted before moving to the next step. Always orient the cuvette in the same
direction when inserting it into the instrument.
5. Insert calibration standard and press ENT. When calibration is
complete, Calibration Completed will be displayed - press ENT. If
ENT is not pressed within 10 seconds the readout will ask if you wish
to abort the calibration - if not, press the down arrow then ENT to
accept the calibration.
6. Insert the Secondary Solid Standard, press either READ button, and record
(attachment) the result. Slight variations in placement of the standard may result
in variations in readings. It is recommended that 3-5 readings be taken while
removing and reinserting the standard between readings. The average of these
readings is used for future checks of instrument stability and for recalibration.
B. With the Secondary Solid Standard.
1. If the instrument was previously calibrated with a known standard and
the value of the Secondary Solid Standard was determined against that value,
you can recalibrate with the Secondary Solid Standard.
2. Follow calibration directions (II.A.1. – 6.).
3. Enter the value found for the Secondary Solid Standard in place of the known
standard.

Jessica M. Barber
Digitally signed by
JESSICA BARBER
Date: 2020.03.10 09:26:49
-04'00'