Turner Designs 10-AU Use and care manual

10-AU Calibration for Chlorophyll

3 Main Steps
1. Setting the basic operating level
2. Blanking the instrument
3. Calibration with a liquid primary standard that has a
known concentration

This procedure should be performed the 1st time you use the
instrument, or any time you switch to a different application,
change filters or lamps.
1. Turn on the 10AU and allow the instrument to warm up
for at least 30 minutes.
#1 Setting the Basic Operating Level

Chlorophyll (ug/l) Rhodamine WT (ppb) Range % FS (+/-5)
240 - 180 100 - 75 High 80%
180 - 160 75 - 65 High 70%
160 - 130 65 - 55 High 60%
130 - 110 55 - 45 High 50%
110 - 80 45 - 35 High 40%
80 - 60 35 - 25 High 30%
60 - 40 25 - 15 High 20%
24 - 18 10 - 7.5 Medium 80%
18 - 16 7.5 - 6.5 Medium 70%
16 - 13 6.5 - 5.5 Medium 60%
13 - 11 5.5 - 4.5 Medium 50%
11 - 8.0 4.5 - 3.5 Medium 40%
8.0 - 6.0 3.5 - 2.5 Medium 30%
6.0 - 4.0 2.5 - 1.5 Medium 20%
2.4 - 1.8 1.0 - 0.75 Low 80%
1.8 - 1.6 0.75 - 0.65 Low 70%
1.6 - 1.3 0.65 - 0.55 Low 60%
1.3 - 1.1 0.55 - 0.45 Low 50%
1.1 - 0.8 0.45 - 0.35 Low 40%
0.8 - 0.6 0.35 - 0.25 Low 30%
0.6 - 0.4 0.25 - 0.15 Low 20%
2. Table 1 is an example of how set the sensitivity of the 10-AU.
Users should determine the appropriate range and %FS
for their calibration standard.
Table 1 is an example
of how to set the
scale for a given
linear range of the
10-AU.
For this example
we are using a
20µg/L standard.

3. Prepare your standards and acetone blank in
test tubes so you are ready to set the sensitivity and
calibrate the instrument.
Be sure to clear past calibrations on screen # 2.6

4. Access screen 2.43 and set range control to manual
and range to the appropriate level for your standard.
For our example the range should be set to MED on
Screen 2.42.
Change to MAN

4. Access Screen 3.2
5. Loosen the sensitivity locking screw using allen wrench.

6. Insert and cover your 20µg/L standard.
7. Using the sensitivity knob slowly turn the knob to adjust
the %FS to the value appropriate for your standard’s
concentration. A deviation ±5% is acceptable.
Sensitivity Knob

8. When the %FS reaches the
designated value, tighten
the sensitivity locking screw.
For our example this is ≈80% of medium
The Basic Operating Level is now set, and you can remove the
standard for the 10-AU.

Setting the Basic Operating Level (aka Sensitivity)
of your 10-AU is now complete.
You can access Screen 2.43 again and return range
control to AUTO and begin calibration.

#2 Blanking the Instrument
Now that the sensitivity is set, you should blank the
instrument.
1. Access screen 2.1
2. Insert and cover your blank (acetone).
Press 1 to Run blank.
*You can also choose to have
the 10-AU subtract the blank
signal from the sample
readings on screen 2.12.

Wait for the readings to stabilize and press “0”.
Wait for 15 seconds for the instrument to blank.
If Blanking is successful you will receive a
“Blank Finished” message

#3 Calibration with Your Standard
Now that the blank is set, you should calibrate with
your standard.
1. Access screen 2.0
2. Press <2> to access screen 2.2 and input the actual
concentration of your standard. Return to screen 2.0
3. Insert and cover your standard.

4. Press 3 to run standard. Wait for the reading to stabilize.
Press the <*> key and the calibration point is set.
Wait 15 seconds and you should receive a “FINISHED”
message.

You can test/check your calibration by running your standards
and other samples or standards.
I ran the 50µg/L and got:
I ran the blank and got:
I ran a 10µg/L standard and got: