VWR SAS Super ISO User manual

VWR®Microbiological Air Sampler
SAS Super ISO
INSTRUCTION MANUAL
European catalogue numbers:
710-0871
710-0872
710-0869
710-0870
Version: 1.2
Issued: July 2016
中国总代理:Nano电子商城
Tel: [phone]
E-mail:[email protected]
http://www.19mro.com

2
Legal address of manufacturer
Europe
VWR International Srl
Via S. Giusto 85
20153 Milano
Italy
Tel.: + 39 0233 203 11
http://it.vwr.com
Country of origin Italy

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Table of contents
Warning........................................................................................................................................ 5
Safety information.......................................................................................................................5
References................................................................................................................................... 5
Technical features.......................................................................................................................6
Intended use................................................................................................................................6
Principle ........................................................................................................................................... 6
The basic idea.................................................................................................................................. 6
Models available .............................................................................................................................. 7
Brief instructions ........................................................................................................................7
Operating instructions for SAS Super ISO 100 and 180 ................................................................. 7
Visual display presentation .............................................................................................................. 8
Installation ................................................................................................................................... 8
The practical use of contact plates .................................................................................................. 8
List of menus and utility sub menus ................................................................................................ 9
Preliminary inspection.................................................................................................................... 10
Holder adjustment.......................................................................................................................... 10
Petri head adapter (optional) ......................................................................................................... 10
Filling 90 mm Petri dishes.............................................................................................................. 10
Tripod installation (optional)........................................................................................................... 10
Function..................................................................................................................................... 11
Software functions ......................................................................................................................... 11
To start with the same air volume as the previous sample ........................................................... 11
“SELECT VOLUME” function (to start with one of the eight memorised volume) ......................... 12
“PROGRAM VOLUME” function (modification of the value of a stored volume)........................... 13
Using a “SET DELAY” programme to delay instrument start ........................................................ 14
“UTILITY MODE” function.............................................................................................................. 15
“SET TIME”.................................................................................................................................... 15
“SITE”............................................................................................................................................. 16
“IDENTIFY” .................................................................................................................................... 17
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“LANGUAGE”................................................................................................................................. 18
“DISPLAY RECORD”..................................................................................................................... 18
“CLEAR RECORD”........................................................................................................................ 19
“REMOTE CONTROL” .................................................................................................................. 19
“CALIBRATION”............................................................................................................................. 19
“AUTOSWITCH” ............................................................................................................................ 20
“MULTI MODE” function ................................................................................................................ 20
“CONFIGURATION” function......................................................................................................... 22
“POWER OFF” function ................................................................................................................. 26
Operations ................................................................................................................................. 26
Adjustable plate holders ................................................................................................................ 26
Troubleshooting ........................................................................................................................27
Accessories...............................................................................................................................27
Technical service ......................................................................................................................29
Warranty.....................................................................................................................................29
Compliance with local laws and regulations.......................................................................... 29
Equipment disposal..................................................................................................................30

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Warning
This document is the property of VWR International S.r.l. - Milan - Italy
It may not be duplicated or distributed without the owner’s authorisation.
Patent Pending
Manufactured by VWR International S.r.l. - Milan, Italy
Safety information
Please use this device only for the purposes indicated.
The device must be correctly used according to this instructions manual before starting any operation.
Any electrical cables need to be replaced immediately when damaged; never use a damaged or worn electrical
cable.
Always disconnect the charger before:
Repairing or maintenance; these operations must be carried out by qualified staff
Cleaning the unit
Use original spare parts and accessories for any replacement.
Do not use this device in the presence of explosive gas.
Please follow the guidelines below and read this manual in its entirety to ensure safe operation of the unit.
Be aware that the voltage and frequency of the electrical system are compatible with the requirements
of the battery charger.
Never use a non OEM charger to charge the air sampler.
Use of an improper charger may damage the unit.
Carefully read this manual before operating your instrument.
References
FDA - 1987 Guideline on Sterile Drug Products produced by Aseptic Process
ACGIH - Guideline for the Assessment of Bioaerosol in the Indoor Environment
ASTM - Draft Protocol - Committee D22.05.06
USP 23-NF 18 8th Supplement 1116 (May 1998) - Microbiological Evaluation of Clean Rooms and other Controlled
Environments
EU Guide for GMP - Manufacture of Sterile Medicinal Products Control of Medicines and Inspection
CEN/TC 243 Norms for Clean Room Technology
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Technical features
Feature SAS ISO 100 Contact SAS ISO 180 Contact SAS ISO 100 Petri SAS ISO 180 Petri
Cat. No. 710-0871 710-0872 710-0869 710-0870
Air flow 100 l/min 180 l/min 100 l/min 180 l/min
Use with 55 mm contact plates 55 mm contact plates 90 mm Petri dishes 90 mm Petri dishes
Portable Yes Yes Yes Yes
Battery Rechargeable Rechargeable Rechargeable Rechargeable
Battery life 70 000 litres 40 000 litres 70 000 litres 40 000 litres
Speed
sensor Yes Yes Yes Yes
Intended use
Principle
The Surface Air System (SAS) encompasses several models which use the same principle.
Air is aspirated at a fixed speed for a variable time through a cover which has been machined with a series of
specially designed small holes. The resulting laminar airflow is directed onto the agar surface of a “contact plate” (or
Petri dish) containing media suitable for the microbiological examination to be performed. When the pre-set
sampling cycle is completed, the plate is removed and incubated. The organisms are then visible to the naked eye
and can be counted in order to assess the level of contamination.
The basic idea
The major points of the Surface Air System (SAS) are:
1. To use a simple and inexpensive “contact plate” (“SURFAIR PLATE”, “RODAC”) for surface, hands or air
control. These plates are very well known and easily available and can be purchased ready poured with
different media.
2. To sample a known volume of air for a variable time to provide a large range of sampling volumes.
3. To aspirate air in a laminar flow pattern with sufficient velocity to impact organisms on an agar surface.
4. To accumulate data on the level of hygiene in each environment so that fluctuations can be monitored.
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5. To take advantage of advanced electronics for more reliable results in different operating conditions.
6. To have the flexibility to choose between 55 mm contact plates or 90 mm standard disposable Petri
dishes.
7. To apply cGLP and cGMP to air sampling operations.
8. To organise sequential sampling to obtain a more representative sample under actual operating
conditions.
Models available
SAS SUPER ISO 100 and SAS SUPER ISO 180: Two instruments for two different applications.
The two air samplers have the same performances with the only difference being in the rate of air aspirated:
SAS SUPER ISO 100 = 100 litres of air per minute.
SAS SUPER ISO 180 = 180 litres of air per minute.
The SAS SUPER ISO 180 is appropriate for use in cleanrooms and other applications demanding fast sampling
times. In cleanrooms, for example, it is important to test larger volumes of air, because microbial air contamination is
very low. The SAS SUPER ISO 180 reduces the time required to obtain a sample.
Both SAS SUPER ISO 100 and SAS SUPER ISO 180 are identified as “SAS SUPER ISO”.
Brief instructions
Brief operating instruction for SAS Super ISO 100 and 180
The airflow can be seen on a digital display at the fourth automatic succession presentation each time the
instrument is switched on.
1. Touch the screen for at least two seconds to switch the instrument on.
2. Press START to sample the same air volume as the last sampling cycle.
3. To change the volume of air, press MENU, to reach “select volume”, then press ENTER and use the arrows to
choose the new desired volume of air.
4. Refer to instruction manual to change settings.
5. Press ENTER to confirm selection.
6. Press MENU and use the arrows for menu selection to modify other pre-set parameters.
7. Press ENTER to reach the sub menu of the chosen parameter.
8. Refer to the instruction manual to change settings.
9. Press STOP each time you need to end an action. The unit will then go back to its initial configuration.
10. Press MENU, and then use the arrows to reach POWER OFF, and then ENTER to switch the unit off.
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3. Replace the aspirating head.
4. Select required volume and start the unit. The airflow is directed into the agar surface of the plate.
5. At the end of the cycle, remove the aspirating head.
6. Close and remove the plate.
7. Incubate.
8. Count the colonies, record the results on the microbiological air sampling report and read the results.
List of menus and utility sub menus
START Press to repeat the same volume of prior sampling
MENU Press to enter the following sub menus
SELECT VOLUME The volume of aspirated air can be modified according to 8 programmed values
PROGRAM VOLUME The 8 programmed values can be modified
SET DELAY The air sampler can be programmed to start sampling after a programmable delay
(1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60 minutes)
MULTIMODE The air sampler can be programmed to extend the total sampling time using
“sequential interval time” sampling by the following sub menus:
INTERVAL TIME
Can be programmed from 1 to 59 min
NUMBERS OF RUNS
Can be programmed from 1 to 19 air aspirations
SINGLE RUN VOLUME
Can be programmed from 1 to 1999 l
TOTAL RUNS
Shows the total volume of aspirated air
CONFIGURATION You can use the SAS with eight programmable configurations
UTILIY MODE
SET TIME
To programme date and time
SITE
To identify the sampling point
IDENTIFY
To identify the operator’s name
LANGUAGE
To display text in different languages
DISPLAY RECORD
To show the recorded sampling data
CLEAR RECORD
To clear the recorded sampling data
REMOTE CONTROL
To connect to a PC with an optional device
CALIBRATION
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To calibrate the volume of aspirated air
AUTOSWITCH-OFF
To disable the autoswitch off
POWER OFF The air sampler can be switched off by pressing the ENTER button
Preliminary inspection
The apparatus is subject to specific working tests before shipping and it is carefully packed to avoid possible
damages during transportation. However, a visible check should be carried out as soon as possible to determine
any transit damage. This must be reported immediately. The following procedures should be followed to check that
the unit is working properly.
The battery pack of the instrument must be charged for at least 5 hours before testing SAS SUPER ISO 100 and
SAS SUPER ISO 180.
After the screen is touched for at least two seconds, an automatic visual presentation appears (see visual display
presentation paragraph).
Press START on the screen to start air sampling with the last cycle. The motor will run until the figures in the
brackets reach the same figure as displayed to the right and then it will stop.
Holder adjustment
Contact plate and the Petri dish holders can be adjusted (using a hex key) in case the available plates are slightly
different in diameter from the standard 55 mm contact plate or 90 mm Petri dish.
Petri head adapter (optional)
An adapter is available in aluminium or stainless steel which allows the use of standard 90 mm Petri dishes with
SAS for contact plates.
Filling 90 mm Petri dishes
We recommendfilling standard 90 mm disposable Petri dishes with no more than 18 - 20 ml of agar to avoid the
medium touching the inside surface of the aspirating head.
Tripod installation (optional)
DATE TIME
[XXXX]‐ >XXXXLt.
START STOP
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The SAS SUPER ISO 100 and SAS SUPER ISO 180 can be fixed to a table/floor tripod or a wall support. The screw
thread connection is located underneath the unit, between the two front feet.
Functions
Software function
Touch the screen for at least two seconds to switch the unit on.
The air sampler is supplied with eight programmable air volumes (“Select volume”) stored in the memory and eight
sampler configuration (“Configuration”) selectable by the operator.
The selection of the volume is made by pressing the “UP” or “DOWN” arrows when the programme in the relevant
sub menu.
The following volumes of air are suggested:
- Contaminated areas (communities, processing rooms, etc) 10 - 200 litres of air
- Normal areas (laboratory benches, houses, etc) 200 - 500 litres of air
- Sterile or high risk areas (cleanrooms, operating theatres, etc) 500 - 1000 litres of air
To start with the same air volume as the previous sample
Switch on the unit by touching the screen for at least two seconds. After the visual display presentation you will see
the following display with the last aspirated volume of air:
Press START on the screen to start air sampling with the last cycle. The motor will run until the figures in the
brackets reach the same figure as displayed to the right and then it will stop.
08/31/06 11.31
500Lt.DELAY00MIN
START MENU
Date
Time
Batterystatus
Lastaspirated
volumeofair
Lastdelay
timeset
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“SELECT VOLUME” function (to start with one of the eight memorised volumes)
Switch the unit on by touching the screen for at least two seconds. After the visual display presentation you will see
the following display with the last aspirated volume of air.
Press MENU and select the SELECT VOLUME function by pressing the “UP” or “DOWN” arrows.
Press ENTER and select a volume by pressing the “UP” or “DOWN” arrows (for example 1250 litres)

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SELECTVOLUME
CLEARENTER

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[1250]Litres
CLEARENTER
08/31/06 11.31
500Lt.DELAY00MIN
START MENU
08/31/06 11.31
TIME
[0023]‐>0500Lt.
START STOP
Currentaspirated
volumeofair
Targetvolume
ofair
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Press ENTER to select the volume and the display will show the selected volume.
Press START to aspirate the selected volume of air.
“PROGRAM VOLUME” function (modification of the value of a stored volume)
Using this procedure, it is possible to memorise up to eight different volumes (from 1 to 1999 litres of air).
Switch the unit on by touching the screen for at least two seconds.
Press MENU and select the PROGRAM VOLUME function by pressing the “UP” or “DOWN” arrows.
Press ENTER to modify the volumes.
Select a volume by “^” pressing the “UP” or “DOWN” arrows and press ENTER.
MENU'
PROGRAMVOLUME
CLEAR ENTER
SELECT VOLUME
[1250]Litres
CLEAR ENTER
MODIFY RUN
[1250]
^
CLEAR ENTER
08/31/06 11.31
1250Lt.DELAY00MIN
START MENU
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Change the digit indicated by pressing the “UP” or “DOWN” arrows and presses ENTER to go to the next figure.
Repeat until you reach the last digit and then confirm by pressing ENTER.
Note: The maximum air volume is 1999 litres.
Using a “SET DELAY” programme to delay instrument start
This function is used to set a delay time before the sampling cycle.
Switch the unit on by touching the screen for at least two seconds.
Press MENU and select SET DELAY function by pressing the “UP” or “DOWN” arrows.
Press ENTER to modify the delay time.
Select the desired delay time by pressing the “UP” or “DOWN” arrows.
The available delay times are 0 – 1 – 2 – 3 – 4 – 5 – 6 – 7 – 8 – 9 – 10 – 20 – 30 – 40 – 50 - 60 minutes.
PressENTERtoconfirm.
When the delay is set (for example 5 minutes), you will see the delay time on the main display.
The message “DELAY” will then flash until the selected time is reached. The display flashes to confirm delayed
start has been selected.
MENU'
SETDELAY
CLEAR ENTER
SET DELAY
[00]Minutes
CLEAR ENTER
08/31/06 11.31
0250Lt.DELAY05MIN
START MENU
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“UTILITY MODE” function
In this programme the following SUB-MENUS are listed: Set time, Site, Identify, Language, Display Record, Clear
Record, Remote Control, Calibration and Autoswitch.
Always start from UTILITY MODE to reach one of these SUB-MENUS.
Switch on the unit by touching the screen for at least two seconds.
Press MENU and select UTILITY MODE function by pressing the “UP” or “DOWN” arrows.
Press ENTER to enter the sub menu.
“SET TIME”
This option is used to programme day, month, year and time of the day.
Press ENTER to modify the time.
08/31/06 11.31
TIME
**DELAY**
START MENU
MENU'
UTILITYMODE
CLEAR ENTER
UTILITY MODE
SETTIME
CLEAR ENTER
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Change the number indicated by “^^” by pressing the “UP” or “DOWN” arrows and press ENTER to go to the next
number.
Repeat until you reach the last number, confirm by pressing ENTER.
“SITE”
This option is used to memorise the name of the site in the file “DISPLAY RECORD”. Site identification should be
changed for samples taken at different sites especially if the results are to be printed.
Select SITE function from UTILITY MODE programme.
Press ENTER to modify the site.
Change the figure indicated by “^” by pressing the “UP” or “DOWN” arrows and press ENTER to go to the next
figure.
MM/DD/YY HH:mm
08/31/06 11:33
^^
CLEAR ENTER
MM/DD/YY HH:mm
08/31/06 11:33
^^
CLEAR ENTER

U
TILITY M
O
DE
SITE
CLEARENTER
SITE
[ABCDEFGH]
^
CLEAR ENTER
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Repeat until you reach the last figure and then confirm by pressing ENTER.
“IDENTIFY”
This option is used to identify the operator. This should be changed if different operators use the sampler and
especially if the date is to be printed. The data is recorded in the file “DISPLAY RECORD”.
Select IDENTIFY function from UTILITY MODE programme.
Press ENTER to modify the identity.
Change the figure indicated by “^” by pressing the “UP” or “DOWN” arrows and press ENTER to go to the next
figure.
Repeat until you reach the last figure and then confirm by pressing ENTER.
SITE
[LABOEFGH]
^
CLEAR ENTER
UTILITY MODE
IDENTIFY
CLEAR ENTER
IDENTIFY
[12345678]
^
CLEAR ENTER
IDENTIFY
[34345678]
^
CLEAR ENTER
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“LANGUAGE”
Menu text can be selected choosing from different languages.
Select LANGUAGE function from UTILITY MODE programme.
Press ENTER to modify the language.
Change the language by pressing the “UP” or “DOWN” arrows and press ENTER to select the desired language;
the display will then show the selected language.
Press CLEAR to exit this function.
“DISPLAY RECORD”
The last 300 samplesare memorised in the file “DISPLAY RECORD”. Each sample is identified in chronological
date order and shows the date, time, operator, site and volume of air sampled (only after a completed run cycle).
Select DISPLAY RECORD function from UTILITY MODE programme.

U
TILITY M
O
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LANGUAGE
CLEARENTER

LA
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A
G
E
ENGLISH 
CLEARENTER

U
TILITY M
O
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DISPLAYRECORD
CLEARENTER
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Press ENTER to display the following data.
You can view all completed cycles by pressing the “UP” or “DOWN” arrows. The symbol “*” is displayed near the
volume aspirated if the volume was aspirated with the multimode function.
Press CLEAR to exit this function.
“CLEAR RECORD”
This option is used to delete all the data memorised in the “DISPLAY RECORD”. Before starting this procedure,
be sure that all stored data are not required or have been downloaded.
Select CLEAR RECORD function from UTILITY MODE programme.
Press ENTER to delete the data.
Press ENTER to confirm the deletion of data or press CLEAR to exit the function.
“REMOTE CONTROL”
The REMOTE CONTROL function is used to connect the SAS to an optional infrared data downloading device.
“CALIBRATION”
The “Calibration” function is a password protected menu accessible only by an authorised technician.
DISPLAY RECORD
001 08/31/06 11.31
BBCD/3345 Lt. 1250 *
CLEAR
Recordposition
number
Date
Site/Identity
Endsamplingtime
AspiratedLiters
Multimodeindicator
UTILITY MODE
CLEARRECORD
CLEAR ENTER
CLEAR RECORD
CONFIRM
CLEAR ENTER
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“AUTOSWITCH”
In order to save battery power, after 5 minutes of inactivity the instrument turns itself off.
With the “Autoswitch” function is possible to disable this feature.
“MULTI MODE” function
This programme is very useful for extending the time of sampling with the purpose of obtaining a more
representative environmental sample “in actual operating conditions” (very important, for example, during surgery).
The total air volume to be sampled is aspirated with two or more sub volume aspirations (e.g. 1000 litres in ten runs
of 100 litres at five minute intervals). Before entering the MULTI MODE programme you should, therefore, decide (a)
total volume of air to be sampled onto the contact plate; (b) number of runs; (c) interval time.
Switch on the unit by touching the screen for at least two seconds.
Press MENU and select MULTI MODE function by pressing the “UP” or “DOWN” arrows.
Press ENTER to enter the multimode function.
Select the desired interval time between sampling by pressing the “UP” or “DOWN” arrows. Press the ENTER
button to go to the next digit.
The interval time can be set from 0 to 59 minutes.
Press ENTER to confirm.
MENU'
MULTIMODE
CLEAR ENTER
INTERVAL TIME
[01]Minutes
^
CLEAR ENTER
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