VWR avantor 710-2088 User manual

VERSION: 2
ISSUED: APRIL 2021
EU Catalogue Number:
710-2088
710-2087
710-2090
710-2089
VWR®Microbiological Air Sampler
SAS Super ISO USB
Instruction manual

2 vwr.com | Instruction manual
Europe
VWR International Srl
Via S. Giusto 85
20153 Milano
Italy
Tel.: + 39 0233 203 11
http://it.vwr.com
Country of origin Italy

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Table of contents
LEGAL ADDRESS OF MANUFACTURER
2
WARNING
4
SAFETY INFORMATION
4
REFERENCES
4
TECHNICAL FEATURES
5
INTENDED USE
5-7
INSTALLATION
7-9
FUNCTIONS
9-14
“OPTIONS” FUNCTION
15-24
OPERATIONS
25
COLONY FORMING UNIT
CORRECTION FACTOR
26
TROUBLESHOOTING
27
FIRMWARE UPDATE
28
ACCESSORIES
29
TECHNICAL SERVICES
30
WARRANTY
30
COMPLIANCE WITH LOCAL LAWS
AND REGULATIONS
30
EQUIPMENT DISPOSAL
31

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Warning!
This document is the property of VWR International S.r.l. - Milan - Italy
It may not be duplicated or distributed without the owner’s authorisation. Patent Pending
Manufactured by VWR International S.r.l. - Milan, Italy
Safety Information
Please use this device only for the purposes indicated.
The device must be correctly used according to this instructions manual before starting any
operation. Any electrical cables need to be replaced immediately when damaged; never use a
damaged or worn electrical cable.
Always disconnect the charger before:
‒Repairing or maintenance; these operations must be carried out by qualified staff
‒Cleaning the unit
Use original spare parts and accessories for any replacement.
Do not use this device in the presence of explosive gas.
Please follow the guidelines below and read this manual in its entirety to ensure safe
operation of the unit.
Be aware that the voltage and frequency of the electrical system are
compatible with the requirements of the battery charger.
Never use a non-OEM charger to charge the air sampler. Use of an improper
harger may damage the unit.
Carefully read this manual before operating your instrument.
References
‒FDA - 1987 Guideline on Sterile Drug Products produced by Aseptic Process
‒ACGIH - Guideline for the Assessment of Bioaerosol in the Indoor Environment
‒ASTM - Draft Protocol - Committee D22.05.06
‒USP 23-NF 18 8th Supplement 1116 (May 1998) - Microbiological Evaluation of Clean Rooms
and other Controlled Environments
‒EU Guide for GMP - Manufacture of Sterile Medicinal Products Control of Medicines and
Inspection
‒CEN/TC 243 Norms for Clean Room Technology

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Technical features
Feature SAS SUPER ISO USB 100 Contact SAS SUPER ISO USB 180 Contact SAS SUPER ISO USB 100 Petri SAS SUPER ISO USB 180 Petri
Cat. No. 710-2088 710-2087 710-2090 710-2089
Air flow 100 l/min 180 l/min 100 l/min 180 l/min
Use with 55 mm contact plates 55 mm contact plates 90 mm Petri dishes 90 mm Petri dishes
Portable Yes Yes Yes Yes
Battery Rechargeable Rechargeable Rechargeable Rechargeable
Battery life 70 000 litres 40 000 litres 70 000 litres 40 000 litres
Speed sensor Yes Yes Yes Yes
Intended use
Principle
The Surface Air System (SAS) encompasses several models which use the same principle.
Air is aspirated at a fixed speed for a variable time through a cover which has been machined
with a series of specially designed small holes. The resulting laminar airflow is directed onto the
agar surface of a “contact plate” (or Petri dish) containing media suitable for the microbiological
examination to be performed. When the pre-set sampling cycle is completed, the plate is
removed and incubated. The organisms are then visible to the naked eye and can be counted in
order to assess the level of contamination.
The basic idea
The major points of the Surface Air System (SAS) are:
1 To use a simple and inexpensive “contact plate” (“SURFAIR PLATE”,
“RODAC”) for surface, hands or air control. These plates are very well
known and easily available and can be purchased ready poured with
different media.
2 To sample a known volume of air for a variable time to provide a
large range of sampling volumes.
3 To aspirate air in a laminar flow pattern with sufficient velocity to
impact organisms on an agar surface.
4 To accumulate data on the level of hygiene in each environment
so that fluctuations can be monitored.
5 To take advantage of advanced electronics for more reliable
results in different operating conditions.
6 To have the flexibility to choose between 55 mm contact plates
or 90 mm standard disposable Petri dishes.
7 To apply cGLP and cGMP to air sampling operations.
8 To organise sequential sampling to obtain a more representative
sample under actual operating conditions.

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Models available
SAS SUPER ISO USB 100 and SAS SUPER ISO USB 180: Two instruments for two different
applications.
The two air samplers have the same performances with the only difference being in the rate of
air aspirated:
SAS SUPER ISO USB 100 = 100 litres of air per minute.
SAS SUPER ISO USB 180 = 180 litres of air per minute.
The SAS SUPER ISO USB 180 is appropriate for use in cleanrooms and other applications
demanding fast sampling times. In cleanrooms, for example, it is important to test larger volumes
of air, because microbial air contamination is very low. The SAS SUPER ISO USB 180 reduces the
time required to obtain a sample.
Both SAS SUPER ISO USB 100 and SAS SUPER ISO USB 180 are identified as “SAS
SUPER ISO USB”.
Brief instructions
Brief operating instruction for SAS Super ISO USB 100 and 180
The airflow can be seen on a digital display at the fourth automatic succession presentation each
time the instrument is switched on.
1 Touch the OK button for at least one seconds to switch the instrument on.
Visual display
(Touch the OK button for at least two seconds).
Each time the instrument is turned on, a five second visual display presentation will appear
displaying the following information.
This information appears in automatic succession.
2 Press OK to sample the same air volume as the last sampling cycle.
3 To change the volume of air, press or to reach “SELECT VOLUME”, then press OK and use
the arrows to choose the new desired volume of air.
4 Refer to instruction manual to change settings.
5 Press OK to confirm selection.
6 Press or , for menu selection to modify other pre-set parameters.
7 Press OK to reach the sub menu of the chosen parameter.
8 Refer to the instruction manual to change settings.
9 Press each time you need to end an action. The unit will then go back to its initial
configuration.
10 Press , and confirm by pressing OK to switch the unit off.

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Installation
The practical use of contact plates
1 Remove the aspirating head.
2 Insert an identified, closed and prepared plate and remove
the plate lid.
3 Replace the aspirating head.
4 Select required volume and start the unit. The airflow is
directed into the agar surface of the plate.
5 At the end of the cycle, remove the aspirating head.
6 Close and remove the plate.
7 Incubate.
8 Count the colonies, record the results on the
microbiological air sampling report and read the results.
List of menus and utility sub menus
OK Press to repeat the same volume of prior sampling (start screen)
In the submenu “OK” provides confirmation of the selected action
or Press to enter the following sub menus
Press to switch off (in the Main Screen), or each time you need to end an action.
SELECT VOLUME The volume of aspirated air can be modified according to 8 programmed values
SELECT MULTI-CYC The air sampler can be programmed (8 programmable configurations) to extend
the total sampling time using “sequential interval time” sampling.
SELECT SITE The sites of aspirating air can be selected according to a maximum of 20 programmed positions
SELECT OPERATOR The operators of aspirating air can be selected according to a maximum of 20 programmed IDs
SET DELAY The air sampler can be programmed to start sampling after a programmable
delay (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes)
OPTIONS To change general settings of the device. See description below
BATTERY Press to see the battery status

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OPTIONS Press OK to change general settings of the device
EDIT VOLUMES
Volumes can be added, modified or deleted, to a maximum of 20 values
EDIT MULTI-CYC
Multiple cycles can be added, modified or deleted, to a maximum of 20 values, parameters
are INTERVAL TIME, NUMBERS OF RUN, SINGLE RUN VOLUME and TOTAL RUNS
EDIT SITES
Sampling sites can be added, modified or deleted, to a maximum of 20 positions
EDIT OPERATORS
Operators can be added, modified or deleted, to a maximum of 20 users
DISPLAY RECORD
To show the recorded sampling data
STORE PDF
To store the recorded sampling data in a PDF file on an USB stick
STORE XML
To store the recorded sampling data in a XML file on an USB stick
CLEAR RECORD
To clear the recorded sampling data
SET TIME
To programme date and time
SET AUTOSWITCH
To disable the auto switch off
PASSWORD ADMIN
To lock the device, still possible to start and stop a sampling procedure
PASSWORD PDF
To lock exported PDF
LOGIN
To use a user management for data integrity
LANGUAGE
To display text in different languages
STORE CONFIG
To store all configuration (Volume, Muli-Cycl, Sites, Operators…) on a USB stick
LOAD CONFIG
To load a config file of an existing Air sampler to implement all setting
(Volume, Multi-Cycle, Sites, Operators,…) on a new device
Preliminary inspection
The apparatus is subject to specific working tests before shipping and it is carefully packed to
avoid possible damages during transportation. However, a visible check should be carried out
as soon as possible to determine any transit damage. This must be reported immediately. The
following procedures should be followed to check that the unit is working properly.
The battery pack of the instrument must be charged for at least 5 hours before testing SAS
SUPER ISO USB 100 and SAS SUPER ISO USB 180.
After OK is pressed for at least two seconds, an automatic visual presentation appears (see visual
display presentation paragraph).
Press OK to start air sampling with the last cycle. The motor will run until the figures in the
brackets reach the same figure as displayed to the right and then it will stop. To stop the
run, press and .

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Holder adjustment
Contact plate and the Petri dish holders can be adjusted (using a hex key) in case the
available plates are slightly different in diameter from the standard 55 mm contact plate
or 90 mm Petri dish.
Petri head adapter (optional)
An adapter is available in aluminium or stainless steel which allows the use of standard
90 mm Petri dishes with SAS for contact plates.
Filling 90 mm Petri dishes
We recommend filling standard 90 mm disposable Petri dishes with no more than 18 - 20
ml of agar to avoid the medium touching the inside surface of the aspirating head.
Tripod installation (optional)
The SAS SUPER ISO USB 100 and SAS SUPER ISO USB 180 can be fixed to a table/floor
tripod or a wall support. The screw thread connection is located underneath the unit,
between the two front feet.
Functions
Software function
Press for at least two seconds to switch the unit on.
The air sampler is supplied with eight programmable air volumes (“Select volume”) stored
in the memory and eight Multi Mode configurations selectable by the operator.
The selection of the volume is made by pressing the “UP” or “DOWN” arrows when the
programme in the relevant sub menu.
The following volumes of air are suggested:
‒Contaminated areas (communities, processing rooms, etc) 10 - 200 litres of air
‒Normal areas (laboratory benches, houses, etc) 200 - 500 litres of air
‒Sterile or high risk areas (cleanrooms, operating theatres, etc) 500 - 1000 litres of air
Press to switch off (from the Main Screen), or each time you need to end an action.

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To start with the same air volume as the previous sample
Press for at least two seconds to switch the unit on. After the visual display
presentation you will see the following display with the last aspirated volume of air:
Press to start air sampling with the last cycle. The motor will run until the figures in the
brackets reach the same figure as displayed to the right and then it will stop.
Current aspirated
volume of air with
progress bar
Date
Last aspirated
volume of air
Time
Target volume of air
If the following display appears, the Login function is activated.
Please use a User ID to login.
For further details please go to the LOGIN section in this manual.