VWR avantor EHP User manual

VWR®Ergonomic
High-Performance
Instruction Manual Pipettors
VWR EHP Fixed Volume
VWR EHP Single-channel Variable Volume
VWR EHP 8-channel Variable Volume
VWR EHP 12-channel Variable Volume
This manual is available in additional
languages at www.vwr.com
PMS 647C
CMYK 96/54/5/27

2 – VWR EHP PIPETTORS Instruction Manual – 3
Table of Contents
1. Product Description ........................................................ 3
2. Packing .................................................................................... 4
3. Pipettor Design ................................................................... 5
4. Safety Recommendations ........................................... 6
5. Specifications ....................................................................... 7
6. Pipettor Operation .......................................................... 10
7. Operating Instructions .................................................. 14
8. Aspiration and Dispensing Instructions ................ 16
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration .......................................... 17
10. Pipettor Maintenance .................................................. 22
11. Troubleshooting ............................................................... 23
12. Spare Parts .......................................................................... 25
13. Technical Service ............................................................. 27
14. Warranty .............................................................................. 27
1. Product Description
Fixed Volume
The VWR Ergonomic High-Performance (EHP) Fixed
Volume Pipettor is a high quality volumetric instrument
that eliminates the risk of erroneous volume setting.
It can accommodate volumes from 5 μL to 1,000 μL
depending on the model.
Nominal Volume
(µL)
Cat. No.
(EU)
Cat. No.
(NA)
5 613-0166 83009-758
10 613-0167 83009-760
20 613-0168 83009-762
25 613-0169 83009-764
50 613-0170 83009-766
100 613-0171 83009-768
200 613-0172 83009-770
250 613-0173 83009-772
500 613-0174 83009-774
1,000 613-0175 83009-776
Variable Volume
The VWR EHP single-channel pipettor isavolumetric
instrument designed to measure and transfer liquids
precisely and safely. It is available in volumes from 0.1μL
to 10,000 μL.
The VWR EHP 8-channel and 12-channel pipettors are
designed for microplate filling. Thepipettors enable pre-
cise and simultaneous delivery of8 or 12 preset volumes
ofliquid. These pipettors are available in four volume
ranges: 0.5-10 μL, 5-50 μL, 20-200 μL, and 50-300μL.
Nominal
Volume (µL)
Cat. No.
(EU)
Cat. No.
(NA)
Range of
Pipettor
Volumes (µL)
Single-channel Pipettors
2 613-5258 89079-960 0.1 - 2
10 613-5259 89079-962 0.5 - 10
20 613-5260 89079-964 2 - 20
50 613-5261 89079-966 5 - 50
100 613-5262 89079-968 10 - 100
200 613-5263 89079-970 20 - 200
250 613-5264 89079-972 50 - 250

4 – VWR EHP PIPETTORS Instruction Manual – 5
3. Pipettor Design
Model Identification
The volume range of the pipettor is shown on the
pushbutton and on the tip ejector bar in multi-channel
pipettors.
Fixed Volume Variable Volume
A1
A2
N
B1
B2
C
D
R
A
F
N
C
D
F
A. Pipetting pushbutton: Consists of 2 parts:
thepushbutton (A1) and the knob (A2).
B1. Volume adjustment knob: Used to set thevolume.
B2. Locking Ring: Allows the volume setting to be
locked by pushing the ring upwards.
C. Shaft: Manufactured using high quality plastic,
ensuring high chemical and mechanical strength.
D. Tip ejector: For multi-channel pipettors, the tips are
ejected sequentially, reducing the required force.
F. Shaft nut
N. Ejector pushbutton
R. Multi-channel manifold: Contains a set of plungers
andaset of flexible-suspension shafts, which func-
tion to reduce the force required to attach the tip.
Nominal
Volume (µL)
Cat. No.
(EU)
Cat. No.
(NA)
Range of
Pipettor
Volumes (µL)
1,000 613-5265 89079-974 100 - 1,000
5,000 613-5266 89079-976 500 - 5,000
10,000 613-5267 89079-978 1,000 - 10,000
8-channel and 12-channel Pipettors
8-10
12-10
613-5250
613-5254
89079-944
89079-952 0.5 - 10
8-50
12-50
613-5251
613-5255
89079-946
89079-954 5 - 50
8-200
12-200
613-5252
613-5256
89079-948
89079-956 20 - 200
8-300
12-300
613-5253
613-5257
89079-950
89079-958 50 - 300
VWR pipettors operate using an air-cushion
(i.e., theaspirated liquid does not come into contact
with the shaft or plunger of the pipettor). The liquid is
drawn into thedisposable tip attached to the pipettor.
2. Packing
The pipettors are delivered with the following:
Description Qty/Pk
Instruction manual 1
Quality Control certificate 1
Calibration key 1
Pipettor hanger 1
Identification labels 1
Lubricant (for single-channel pipettor) 1
Ejector cap
(for pipettor models 2, 5, 10, 5,000, and 10,000 μL) 1
Filters (for pipettor models 5,000 μL and 10,000 μL) 3
Ejector spacers (for models up to 1,000 µL) 2
Filters
The 5,000 μL and 10,000 μL pipettors include areplace-
able filter, fitted at the bottom of the shaft which
prevents aspirated liquid from entering the shaft and
contaminating the inner surface and plunger. Use of
the filter is particularly important when aspirating and
dispensing large volumes ofliquid. The filter should be
replaced if it becomes wet.

6 – VWR EHP PIPETTORS Instruction Manual – 7
5. Specifications
The pipettor is a high quality instrument which offers
excellent accuracy and precision. The accuracy and
precision (repeatability) of the liquid volume depends on
the quality of pipet tips used. The values for accuracy and
precision shown in the table below were obtained using
manufacturer non-filter pipet tips. Those tips are recom-
mended for use to ensure compatibility, accuracy and
precision when pipetting.
Fixed Volume
Nominal
Volume
(µL)
Accuracy
(%)
Precision
(%)
Non-filter
Tips
(μL)
5 ± 1.0 ≤ 0.8 10
10 ± 0.5 ≤ 0.4 10
20 ± 0.8 ≤ 0.4 200
25 ±0 .8 ≤ 0.4 200
50 ± 0.8 ≤ 0.4 200
100 ± 0.8 ≤ 0.3 200
200 ± 0.6 ≤ 0.3 200
250 ± 0.6 ≤ 0.3 1,000
500 ± 0.5 ≤ 0.3 1,000
1,000 ± 0.4 ≤ 0.3 1,000
4. Safety Recommendations
Long-term use of the pipettor relies on correct method
of use. Please read and follow the instructions for use
carefully. Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors
in pipetting.
NOTE:
•The pipettor is designed for the transfer of liquids only
using the tip. Do not aspirate liquids without the tip
attached. The aspirated liquid should not enter the
pipettor, asit may cause damage.
•Single-use tips reduce the risk of contamination
of samples.
•Keep the pipettor clean, avoiding the use of abrasive
or corrosive cleaning agents (e.g., acetone).
•Keep the pipettor upright when there is liquid in
the tip.
•Only using the pipettor in accordance with the manu-
facturer’s instructions ensures the correct pipettor
parameters are maintained.
•After replacing the plunger or the shaft, the pipettor
should be calibrated.
•In the case of incorrect operation, the device should
be cleaned in accordance with the Instructions for
Use ortransferred to a service point.
•Ambient operating temperature is +5°C to 45°C.
•Ambient storage conditions (in the original packaging
during transport and short storage) is -25°C to 55°C.
When working with the pipettor:
•Follow general work safety regulations regarding
hazards related to work in the laboratory.
•Take special care when pipetting aggressive
substances.
•Use appropriate protective attire (e.g., clothing,
goggles, and gloves).
•Avoid pointing the pipettor at yourself or others
during use.
•Only use parts and accessories recommended
by the manufacturer.

8 – VWR EHP PIPETTORS Instruction Manual – 9
Nominal
Volume
(µL)
Volume
(μL)
Accuracy
(%)
Precision
(%)
Non-filter
Tips
(μL)
8-channel and 12-channel Pipettors
8 - 10
12 - 10
Min. 0.5 ±10.0 ≤8.0
10
1±8.0 ≤6.0
5±4.0 ≤2.0
Max. 10 ±2.0 ≤1.2
8 - 50
12 - 50
Min. 5 ±4.0 ≤2.5
20025 ±3.0 ≤1.2
Max. 50 ±1.6 ≤0.6
8 - 200
12 - 200
Min. 20 ±3.0 ≤1.5
200100 ±1.5 ≤0.8
Max. 200 ±1.0 ≤0.6
8 - 300
12 - 300
Min. 50 ±1.6 ≤1.5
300150 ±1.2 ≤1.0
Max. 300 ±1.0 ≤0.6
The accuracy and precision were obtained gravi-
metrically using manufacturer tips performing at least
10measurements ofdistilled water at a temperature of
20°C ± 1°C according to ENISO 8655 standards.
The use of tips from other manufacturers or filter tips
may result in incorrect liquid aspiration and require
pipettor recalibration.
The design of the pipettor enables the user to reca-
librate it according to the information presented in
Section 9.
Variable Volume
Nominal
Volume
(µL)
Volume
(μL)
Accuracy
(%)
Precision
(%)
Non-filter
Tips
(μL)
Single-channel Pipettors
2
Min. 0.1 ±40.0 ≤12.0
10
0.2 ±12.0 ≤6.0
1 ±2.7 ≤1.3
Max. 2 ±1.5 ≤0.7
10
Min. 0.5 ±4.0 ≤2.8
10
1.0 ±2.5 ≤1.8
5.0 ±1.0 ≤0.6
Max. 10.0 ±0.5 ≤0.4
20
Min. 2 ±3.0 ≤1.5
20010 ±1.0 ≤0.5
Max. 20 ±0.8 ≤0.3
50
Min. 5 ±2.5 ≤2.0
20025 ±1.0 ≤0.6
Max. 50 ±0.8 ≤0.4
100
Min. 10 ±1.6 ≤0.80
20050 ±0.8 ≤0.24
Max. 100 ±0.8 ≤0.20
200
Min. 20 ±1.2 ≤0.60
200100 ±0.8 ≤0.25
Max. 200 ±0.6 ≤0.20
250
Min. 50 ±1.0 ≤0.4
300125 ±0.8 ≤0.3
Max. 250 ±0.6 ≤0.3
1,000
Min. 100 ±1.6 ≤0.40
1,000500 ±0.7 ≤0.20
Max. 1,000 ±0.6 ≤0.15
5,000
Min. 500 ±1.2 ≤0.50
5,000
1,000 ±0.6 ≤0.25
2,500 ±0.6 ≤0.20
Max. 5,000 ±0.5 ≤0.15
10,000
Min. 1,000 ±2.5 ≤0.6
10,0005,000 ±0.8 ≤0.3
Max. 10,000 ±0.5 ≤0.2

10 – VWR EHP PIPETTORS Instruction Manual – 11
•Never draw liquids directly into the pipettor without
thetip attached.
Adjusting the tip ejector
The tip ejector can be adjusted by the user and can
accommodate most types of tip available on the
market. When using narrow tubes, it may be necessary
to remove the tip ejector.
2-1,000 μL pipettors
1. Tip ejector disassembly
•Press the tip ejector pushbutton.
•Slide the ejector from the plastic arbor.
2. Tip ejector assembly
•Press the tip ejector pushbutton.
•Line the metal tip ejector up with the plastic arbor.
•Slide the ejector on the arbor.
3. Tip ejector adjustment
•Remove the tip ejector pushbutton.
•Remove “H0” spacer that is inserted on the ejector
arbor. To increase the ejector length use ”H” spacers,
which allow for regulating the length of tip ejector
by +1 mm (H1) or +2 mm (H2). The outside shape
identifies the spacers.
6. Pipettor Operation
Attaching the Tips
•Attach the correct tip corresponding to the model
number displayed on the pipettor pushbutton
(Section5).
•Position the pipettor vertically when attaching tips.
•Single-channel pipettors: push the pipet tip on firmly
using a slight twisting motion to ensure an airtight
seal.
•Multi-channel pipettors: press the pipettor against the
tips positioned in the rack box until the shaft retracts
approximately 1.5mm into the manifold. The suspen-
sion system ensured even and airtight sealing of the
pipet tips. The rocking motion does not have to be
performed to seal the pipet tips tightly.
NOTE:
•Do not attach the tips with a rocking movement,
as this may damage the shaft or plunger. Observe this
rule particularly with single-channel pipettors of low
volume range.

12 – VWR EHP PIPETTORS Instruction Manual – 13
If the tip ejector cannot be adjusted sufficiently, or if the
diameter of the ejector is insufficient to eject the tip, it
may be necessary to attach the ejector cap “M” to the
ejector.
In 2 and 10 μL pipettors place the cap, supplied with the
pipettor, on the bottom of the pipettor shaft and slide
thecap upwards to the bottom of the tip ejector.
5
K
P
P
6
6A
1-1000 µl
5A
5B
6B
5000 µl, 10000 µl
6C
5000 µl, 10000 µl
6D
2 µl, 10 µl
MM
Volume Setting
Variable Volume Pipettors
123
1. To enable volume selection, set the locking ring
to the lower position.
2. Aspiration volume can be set by either turning the
pipetting pushbutton knob, or the adjustment knob.
The volume setting is displayed as a three digit coun-
ter, which should be read from top to bottom. The
smallest volume increment is printed on the bottom
counter drum.
3. After the volume has been set, set the locking ring
to the upper position to avoid inadvertent changing
of volume.
5,000 and 10,000 μL pipettors
1. Tip ejector disassembly
•Remove the tip ejector pushbutton.
•Using a screwdriver, turn the metal arbor counter-
clockwise to unscrew the ejector from the arbor.
•Release the ejector.
2. Tip ejector assembly
•Remove the tip ejector pushbutton.
•Using a screwdriver, turn the metal arbor clockwise
to secure the ejector and adjust its length.
NOTE: Leave a space of at least 1 mm between
the shaft and tip ejector collar.
3. Tip ejector adjustment
•Remove the ejector pushbutton.
•Use the screwdriver to increase or decrease the
ejector length.
•Following ejector assembly or adjustment, ensure
the ejector pushbutton is relocated.

14 – VWR EHP PIPETTORS Instruction Manual – 15
•The pipettor should be held in a vertical position.
•The pipet tip should be changed whenever the volume
setting is altered, and when a different liquid is to be
aspirated.
•The pipet tip should be changed if a droplet remains
on the end ofthe tip from the previous pipetting
operation.
•Each new pipet tip should be pre-rinsed with the liquid
to be pipetted.
•Liquid should never enter the pipettor shaft.
To prevent this:
– Press and release the pushbutton slowly
and smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there
is liquid in the tip.
•Never force the volume setting beyond therecom-
mended limits.
•When pipetting liquids with a different temperature
from the ambient temperature, it is recommended to
pre-rinse the tip several times prior to use.
•Do not pipettor liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions
which emit vapors, it is recommended to disassemble
the shaft and rinse the plunger and O-ring with distilled
water after finishing the pipetting operation.
Examples of counter indications
The decimal point in
the volume of aspi-
rated liquid (μL or mL)
is indicated by change
in color of digits.
Black digits represent
integer numbers, and
red digits represent
decimal fractions
(sizes up to 20 μL).
1
2
5
0
3
5
0
7
5
Volume Ranges 0.1-2 μL 5-50 μL 100-1,000 μL
Set Volume 1.25 μL 35 μL 750 μL (0.75 mL)
Increment 0.002 μL 0.2 μL 2.0 μL
For maximum accuracy, the set volume must be
approached from a higher value by decreasing counter
readings. Before reaching the desired value, reduce the
speed of turning the adjustment knob to avoid inadver-
tently exceeding the desired value.
7. Operating Instructions
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid
sampling.
•During operation, the volume setting should be locked,
with black adjustment knob in the lower position.
•Ensure smooth and slow operation of the pipettor.
•Immersion of the tip into the sample liquid should be
kept to a minimum depth, which should remain con-
stant during aspiration. The recommended immersion
depths are given in the table below:
Model Volume Range
(µL)
Immersion Depth
(mm)
0.1-1 ≤1
1-100 2-3
101-1,000 2-4
5,000 3-6
10,000 5-7

16 – VWR EHP PIPETTORS Instruction Manual – 17
Aspirating High-density Liquids
When pipetting liquids of higher viscosity or lower sur-
face tension than water (e.g., sera or organic solvents),
a film of liquid may be formed on the inside of the
pipet tip which may produce erroneous results. As the
film remains relatively constant in successive pipetting
operations with the same tip, this error can be elimi-
nated by pre-rinsing the tip and allowing a film to form
before transferring the first sample. This is achieved by
aspirating asample and dispensing it back into the
same vessel. Allowing a film to form prior to sampling
ensures optimal accuracy and repeatability.
This pre-rinsing operation should be repeated when
thevolume to be aspirated is changed or when a new
pipet tip is used.
NOTE: Normally the degree of error resulting from
viscous liquids is negligible if pipetting is performed
slowly and carefully, however can be minimized further
by holding the pipet tip in position for at least 2 seconds
after aspiration to allow the liquid time to react to the
change in pressure before it is dispensed.
If the above method does not result in accurate values,
recalibrate the pipettor in accordance with Section 9.
It is recommended to record recalibration and correc-
tion values, in order to facilitate reverse calibration to
astandard liquid.
Filters
The 5,000 μL and 10,000 μL pipettors include a replace-
able filter (L), fitted at the bottom of the shaft which
prevents aspirated liquid from entering the shaft and
contaminating the inner surface and plunger. Use of
the filter is particularly important when aspirating and
dispensing large volumes of liquid. The filter should be
replaced if it becomes wet.
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration
The pipettors have been factory-calibrated using
gravimetric methods with manufacturer pipet tips and
distilled water, in accordance with ISO 8655 guidelines
for the maximum (nominal) liquid volume drawn by the
pipettor and for 10% of the maximum or minimum liquid
volume according to the values given (Section 5).
The pipettors are designed to enable recalibration and
adaptation to different pipetting techniques and liquid
properties (e.g., temperature, density, and viscosity).
8. Aspiration and Dispensing Instructions
1 2 3 4 5 6
Aspirating Liquid
1. Press the pushbutton to the first stop point.
Holdingthe pipettor vertically, immerse the pipet tip
into the sample liquid to the recommended depth
(for recommended values see Section 7). Ifthepipet
tip is not immersed to the recommended depth or if
the pipetting pushbutton is rapidly released, air may
enter the pipet tip.
2. Release the pipetting pushbutton slowly and
smoothly to aspirate the sample. Wait one second
and then withdraw the pipet tip from the liquid.
CAUTION: Do not touch the used tip.
Dispensing Liquid
3. Place the end of the pipet tip against the inside wall
of the vessel at an angle of 10° to 40°. Press the push-
button smoothly to the first stop. Wait one second.
4. Press the pushbutton to the second stop to expel
any remaining liquid. While keeping the pushbutton
depressed, remove the pipettor from the vessel by
drawing the pipet tip against the inside surface of
thevessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector
pushbutton.
Remember to change the pipet tip whenever
a different type of liquid is to be sampled.

18 – VWR EHP PIPETTORS Instruction Manual – 19
•When calculating the liquid volume aspirated by
thepipettor, the conversion factor (Z) (μL/mg) for dis-
tilled water or a liquid with comparable density should
be taken into account. Sample values of conversion
factors are given in the following table.
Temperature
(°C)
Pressure (kPa)
95.0 101.3 105.0
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040
See ISO 8655 for the full table of conversion factors (Z).
NOTE: Pipetting should be performed in accordance
with guidance described in Sections 7 and 8.
Checking the Pipetting Accuracy Parameters
Fixed Volume
Nominal
volume
(µL)
Permissible values
(μL)
Volume Change ΔV
(μL) for a Turn of
a Calibration Key by
1
Turn
1
Increment
54.95 5.05 0,33 0.0137
10 9.95 10.05 0,33 0.0137
20 19.84 20.16 0,63 0.0262
25 24.80 25.20 0,63 0.0262
50 49.60 50.40 2,50 0.104
100 99.20 100.80 2,50 0.104
200 198.80 201.20 6,30 0.262
250 248.50 251.50 25,0 1.04
500 497.50 502.50 25,0 1.04
1,000 996.00 1,004.00 25,0 1.04
Periodic checks of the operation of the pipettor are
recommended at least once per year. Frequency of
checks should be increased depending on workload,
sterilization or autoclave processes, and frequency of
replacement of component parts.
If during pipettor operation the accuracy error (the
difference between the real aspirated volume and the
preset volume) exceeds the permissible value given
inthetable in Section 5, pipettor recalibration should be
carried out.
Recalibration of the pipettor involves setting the counter
to the value of the liquid volume obtained by weighing.
Recalibration of the pipettor is valid only for one liquid
volume drawn by the pipettor.
The recommended volume for recalibration is 10%
of the maximum (nominal) value or minimum volume
depending on which of these values is greater. See table
on page 18 for more details.
Parameters for Checking the Pipetting Accuracy
The pipetting accuracy is influenced by factors such as:
tips used, characteristics of the pipetted liquid (density,
viscosity), and operating conditions (ambient tempera-
ture, pressure).
To determine the accuracy error of the pipettor, the
following conditions should be met:
•Ambient temperature and temperature of the pipet-
tor, pipet tips, and liquid should be within the range
of 20°C to 25°C and stabilized during weighing within
±0.5°C.
•Measurements should be conducted using distilled
water.
•Balance sensitivity should be suitable for the volume
“V” to be measured:
Volume Checked
(V, μL)
Balance Sensitivity
(mg)
0.1≤V≤10 0.001
10≤V≤100 0.01
100≤V≤1,000 0.1
V>1,000 0.1

20 – VWR EHP PIPETTORS Instruction Manual – 21
Pipettor Recalibration
K
12
34
1. Remove the pipetting pushbutton.
CAUTION: In the variable pipettors the pipetting
pushbutton consists of 2 parts: the knob and the
pushbutton. After removal of the pushbutton, both
parts are separated.
2. Insert the calibration key into the calibration screw.
CAUTION: In variable pipettors hold the volume
setting knob to prevent rotation.
3. Turn the key clockwise to reduce the aspirated
volume, or counter-clockwise to increase the aspi-
rated volume. Use the values given in the above table
to precisely adjust the volume.
4. Remove the key and replace the pipetting pushbutton
by first replacing the knob, then the pushbutton.
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties consid-
erably different from those of water, follow theguidance
given in Section 5.
More information on the calibration procedure can be
found at www.vwr.com
Variable Volume
•Set the preset volume depending on the pipettor
volume according to the following table.
Nominal
volume
(µL)
Preset
volume
(μL)
Permissible
values
(μL)
Volume Change ΔV
(μL) for a Turn of
a Calibration Key by
1
Turn
1
Increment
Single-channel Pipettors
20.2 0.176 - 0.224 0.06 0.0025
10 1 0.975 - 1.025 0.33 0.0137
20 2 1.94 - 2.06 0.63 0.0262
50 5 4.875 - 5.125 2.50 0.104
100 10 9.84 - 10.16
200 20 19.76 - 20.24 6.30 0.262
250 50 49.5 - 50.5
1,000 100 98.4 - 101.6 25.00 1.04
5,000 500 494 - 506 125.00 5.2
10,000 1,000 975 - 1,025 250.00 10.4
8-channel and 12-channel Pipettors
10 1 0.92 - 1.08 0.33 0.0137
50 5 4.8 - 5.2 1.67 0.070
200 20 19.4 - 20.6 6.30 0.262
300 50 49.2 - 50.8 10.00 0.42
•Perform 10 aspirations, and calculate the average
value in (mg).
•Calculate the volume in (μL) by multiplying the value
in(mg) by the conversion factor Z (μL/mg).
If the average aspirated volume exceeds the permissible
values of the range, the pipettor should be recalibrated.

22 – VWR EHP PIPETTORS Instruction Manual – 23
Precision and accuracy should not alter if the pipet-
ting processes including autoclaving are carried out as
described in this manual. If a change in accuracy occurs,
itis recommended to:
•Check the calibration of the pipettor after the first,
third, and fifth autoclaving cycles and then after every
10autoclaving cycles.
Ultra Violet (UV) Sterilization
The pipettors are UV resistant. The distance from the
radiation source to the exposed element of the pipet-
tor should be at least 50 cm. Prolonged or intense UV
exposure can cause discoloration of pipettor parts but
does not affect its performance.
11.Troubleshooting
If a problem is encountered during pipettor operation,
use the following table to identify and eliminate the
fault following the instructions provided. Replacement of
parts should be required only occasionally and should
not be required with normal pipettor use.
Problem Cause Solution
Droplets of
liquid remain
in the pipet
tip.
The pipet tip is emptied
too fast.
Decrease the speed
of pressing the
pipettor pushbutton.
The pipet tip wettability
has increased due to
extensive use.
Replace the tip
with a new one.
Droplets
of air
appear in
the liquid
aspirated
into the
pipet tip.
The pipet tip immersion
depth is too shallow.
Immerse the tip
to the recommended
depth according to
the instructions.
The pipet tip is incor-
rectly pressed onto the
pipettor shaft.
Press the pipettor
tip firmly onto the
pipettor.
The tip is damaged
or worn out due to
extensive use.
Replace the tip
with a new one.
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The pipettor tip is incor-
rectly pressed onto the
pipettor shaft.
Press the pipet
tip firmly onto
the pipettor.
10. Pipettor Maintenance
Depending on the applications and intensity of use,
the pipettor requires periodic maintenance. The com-
ponents exposed to corrosive vapors, such as shaft
elements, should be regularly checked and cleaned.
Do not use sharp tools for pipettor maintenance.
Itmay cause damage to the device and affect
the user’s safety.
Cleaning
External surfaces of the pipettor such as the pushbut-
ton, ejector pushbutton, handgrip, shaft nut, and
adjustment knob may be cleaned using a cloth damp-
ened in isopropyl alcohol. The remaining parts removed
from the pipettor during pipettor disassembly may be
washed with distilled water or isopropyl alcohol.
NOTE: Before using cleaning agents other than those
recommended by the manufacturer, check the compat-
ibility charts and consider chemical resistance of the
following plastics which form components of the pipet-
tor: PP, PC, POM, PA, PPS, PVDF.
Sterilization
Sterilization using an autoclave
The pipettor can be sterilized in an autoclave at 121°C
for 20 minutes. Sterilization under other conditions may
cause damage to the pipettor. It is recommended to:
•Unscrew the shaft nut slightly in the 2-1,000 µL pipet-
tors, and unscrew the shaft slightly in the 5,000 and
10,000 µL. After autoclaving these parts should be
screwed tight again.
•Set the locking ring to the lower (unlocked) position
prior tosterilization.
•Sterilize the pipettors using an autoclave with an initial
vacuum and drying cycle.
•After sterilization, the pipettor should be dried and
cooled to room temperature.
•The shaft of the 5,000 and 10,000 µL models should
be autoclaved without the filter.

24 – VWR EHP PIPETTORS Instruction Manual – 25
12. Spare Parts
Contact our Customer Service to inquire about avail-
ability of spare parts. Pipettor model and name of the
part required should be specified. The most common
parts are depicted below.
NOTE: The replacement of the plunger requires conduct-
ing the calibration procedure according to Section 9.
Fixed Volume Variable Volume
A
N
F
G
J*
I
C
D
A
N
F
G
J*
I
C
D
L
*Seal (J) used only in models 2, 5,000 and 10,000 µL
Item Description
Model
Variable
Model
Fixed Cat. No.
Qty
/Pk
B
Volume
adjustment
knob set
All - SP29327 1
C,
I,
J*
Shaft
with O-ring
and seal
2 - SP19131 1
10 5, 10 SP19132 1
20 20, 25 SP19133 1
50, 100 50, 100 SP19134 1
200, 250 200 SP19135 1
1,000 250-1,000 SP19136 1
5,000 - SP19138 1
10,000 - SP19139 1
Problem Cause Solution
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The shaft surface is
damaged or contami-
nated at the sealing site.
Clean the shaft or
replace it with a new
one.
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The plunger or the
O-ring is damaged due
toprolonged aspiration
ofcorrosive liquids.
Disassemble the
shaft set; wash the
shaft, the plunger
and the seal (Section
10: Cleaning).
Replace the ele-
ments with new ones
if necessary. Apply
a small amount of
lubricant onto the
plunger and reas-
semble the set inthe
correct order.
The inside of the pipet-
tor is contaminated.
The sealing elements
are not sufficiently
lubricated.
Uneven work
of the pipet-
ting set, the
pipetting
pushbut-
ton gets
blocked.
The inside of the pipet-
tor is contaminated due
to aspiration of corrosive
substances. Unscrew the shaft
set, wash the parts.
Replace the ele-
ments with new ones
if necessary. Apply
a small amount of
lubricant on the
plunger and reas-
semble the set inthe
correct order.
The inside of the pipet-
tor is contaminated due
to the liquid entering the
pipettor.
The sealing elements
are not sufficiently
lubricated, e.g. after
repeated autoclaving
procedures.
Incorrect
aspiration.
Liquid with properties
other than water
(density, viscosity).
Calibrate the
pipettor using the
liquid which is to be
pipetted.
Tips with a filter
with increased flow
resistance.
Calibrate the pipet-
tor using the tips
which are to be used
for pipetting.
If the problem persists after carrying out the above
steps, contact our regional representative.
Before returning the pipettor, please ensure the pipettor
is completely free of any chemical, radioactive, or micro-
biological contamination which could pose athreat
during transport and repair.

26 – VWR EHP PIPETTORS Instruction Manual – 27
13. Technical Service
Web Resources
Visit the VWR website at www.vwr.com for:
•Complete technical service contact information
•Access to the VWR Online Catalogue, and information
about accessories and related products
•Additional product information and special offers
Contact us
For information or technical assistance contact your
local VWR representative or visit www.vwr.com
14. Warranty
VWR warrants that this product will be free from defects
in material and workmanship for a period of three (3)
years from date of delivery. If a defect is present, VWR
will, at its option and cost, repair, replace, or refund the
purchase price of this product to the customer, provided
it is returned during the warranty period. This warranty
does not apply if the product has been damaged by
accident, abuse, misuse, or misapplication, or from
ordinary wear and tear. If the required maintenance and
inspection services are not performed according to the
manuals and any local regulations, such warranty turns
invalid, except to the extent, the defect of the product is
not due to such non-performance.
Items being returned must be insured by the customer
against possible damage or loss. This warranty shall be
limited to the aforementioned remedies.
IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL
BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN
LIEU OF THE WARRANTY OF MERCHANTABILITY.
DTip ejector
2, 10 5, 10 SP29307 1
20 20, 25 SP29308 1
50, 100 50, 100 SP29309 1
200, 250 200 SP29310 1
1,000 250-1,000 SP29322 1
5,000 - SP19452 1
10,000 - SP19453 1
FShaft nut 2-1,000 All SP29435 1
GPiston
assembly
2 - SP19381 1
10 5, 10 SP19382 1
20 20, 25 SP19383 1
50, 100 50, 100 SP19384 1
200, 250 200 SP19385 1
1,000 250-1,000 SP19386 1
5,000 - SP19388 1
10,000 - SP19389 1
I,
(J)
O-ring
(and seal*)
2 - SP19101 10
10 5, 10 SP19102 10
20 20, 25 SP19103 10
50, 100 50, 100 SP19104 10
200, 250 200 SP19105 10
1,000 250-1,000 SP19106 10
5,000 - SP19108 5
10,000 - SP19109 5
KCalibration
key
All - SP29334 1
- All SP9233 1
LShaft filter 5,000,
10,000 - SP19476 10
MTip ejector
cap
2, 10 5, 10 SP19378 3
5,000 - SP19466 3
10,000 - SP19467 3
SShelf clip All All SP19483 1
*Seal (J) used only in models 2, 5,000 and 10,000 µL

For additional product or technical information, visit
www.vwr.com or contact your local sales office.
Manufactured for VWR International, LLC
100 Matsonford Rd, Radnor, PA 19087
Version 1
Made in Poland
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