Zeiss Mechanical stage S mot. User manual

Operating Manual
Mechanical / Measuring stage S mot.
for
Axio
Zoom and
SteREO
Discovery

You have purchased a high quality product from Zeiss. Before using
the instrument the first time, please read this operating manual in
order to maintain the high quality of the instrument and ensure
reliable work with it for a long time.

COPYRIGHT Mechanical stage S mot. CAN
Carl Zeiss Measuring stage S mot. CAN
4 435465-9000-701 08/2012
Knowledge of this manual is required for the operation of the device. You should therefore familiarize
yourself with the contents of this manual and pay special attention to instructions concerning the safe
operation of the instrument.
We reserve the right to make changes in the interest of technological advancement; the operating
manual is not subject to updating or revision.
© Unless explicitly authorized, dissemination or duplication of this document and commercial
exploitation or communication of its contents are not permitted. Persons in contravention of this
copyright shall be liable to pay compensation for damages.
All rights reserved in the event of a patent grant or registration of a utility patent.
Published by: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
microscop[email protected]m
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
SAP No.: 435465-9000-701
Date of issue (Version 3): 08/09/2012

Mechanical stage S mot. CAN CONTENTS
Measuring stage S mot. CAN Carl Zeiss
08/2012 435465-9000-701 5
CONTENTS
Page
1INTRODUCTION ........................................................................................................... 6
1.1 General information....................................................................................................... 6
1.2 Notes on instrument safety ............................................................................................ 7
1.3 Notes on warranty ......................................................................................................... 9
2DESCRIPTION ............................................................................................................. 10
2.1 Indication for use ......................................................................................................... 10
2.2 Package check list ........................................................................................................ 10
2.3 Accessories and optional components .......................................................................... 11
2.4 Microscope system with mechanical stage S 150x100 mot. CAN ................................... 12
2.5 Technical data.............................................................................................................. 13
3SET-UP ....................................................................................................................... 14
3.1 General information..................................................................................................... 14
3.2 Mounting the mechanical / measuring stage S mot. CAN .............................................. 14
3.2.1 Mounting the mechanical / measuring stage S mot. CAN on base 300 for
epi-illumination............................................................................................................ 15
3.2.2 Mounting the mechanical / measuring stage S mot. CAN on stand base 450 for
epi-illumination............................................................................................................ 16
3.2.3 Mounting the mechanical stage / measuring stage S mot. CAN on the
transillumination base 300 ........................................................................................... 17
3.2.4 Mounting the mechanical / measuring stage S mot. CAN on the
transillumination top DL 450 mot. ................................................................................ 18
3.3 Connecting the mechanical / measuring stage S mot. CAN ........................................... 19
4OPERATION ............................................................................................................... 20
4.1 Switching on and off.................................................................................................... 21
4.2 Control elements on the mobile coaxial drive................................................................ 21

INTRODUCTION Mechanical stage S mot. CAN
Carl Zeiss General information Measuring stage S mot. CAN
6 435465-9000-701 08/2012
1INTRODUCTION
1.1 General information
The mechanical / measuring stage S 150 x 100 mot. CAN and the appropriate controller EMS 3 have
been designed, manufactured and tested in compliance with DIN EN 61010-1 (IEC 61010-1) Safety
requirements for electrical equipment for measurement, control and laboratory use.
Mechanical / measuring stage S 150 x 100 mot. CAN satisfies the requirements of the European
directives 2006/95/EC “Low Voltage Directive”, 2004/108/EC “Electromagnetic Compatibility” and
2006/42/EC “Machinery Directive” with the appropriate controller EMS 3.
The operating manual contains all information and warnings the operator must comply with.
The following warning and information symbols are used in this operating manual:
CAUTION
This symbol indicates a potential hazard to the user.
CAUTION
Optical radiation is emitted. Do not look into the laser beam!
This may be harmful to the eyes (see Section 2.4).
CAUTION
Risk of pinching!
CAUTION
Hot surface!
CAUTION: High-energy UV radiation!
Risk of injury to eyes and skin!
CAUTION
Disconnect the instrument from the power supply before opening!
ATTENTION
This symbol indicates a potential hazard to the instrument or system.
NOTE
This symbol indicates an instruction which requires particular attention.

Mechanical stage S mot. CAN INTRODUCTION
Measuring stage S mot. CAN Notes on instrument safety Carl Zeiss
08/2012 435465-9000-701 7
1.2 Notes on instrument safety
The mechanical stage / measuring stage S mot. CAN may only be used for the applications
described in this operating
manual. The manufacturer cannot assume any liability for other
applications of the instrument, including those of individual modules or single parts.
Modifications and repairs to this instrument and any devices operated in combination with the
microscop
e must be carried out by our service department or by authorized personnel only. The
manufacturer accepts no liability for damage caused by unauthorized access to the interior of
the instrument. Failure to comply with this shall also render any warranty claims invalid.
The device may only be operated by instructed personnel. The instruments may only be
operated by trained personnel who are aware of the possible dangers involved with microscopy
and the application concerned. The microscope is a high-
precision instrument that can be
impaired in its performance or destroyed when handled improperly.
The mains plug of instruments with a metal housing (e.g. EMS 3) may only be connected to a
socket with earth contact. The protective capacity must not be rendered ineffective by using an
extension cable without a ground wire.
If it is determined that protective measures are no longer effective, the instrument must be
switched off and secured against inadvertent operation. Please contact a Zeiss
service agency or
the Carl Zeiss Microscopy Service to repair the instrument.
Dust and dirt may impair the instrument’s performance. The instrument must be effectively
protected from such influences and covered with the dust cover when not in use. Alway
s check
whether the instrument is switched off before you cover it. Broad temperature fluctuations,
direct sunlight and vibrations should be avoided.
The instruments are not equipped with special devices to protect against corrosive, potentially
infec
tious, toxic, radioactive or other substances that may be hazardous to health. All legal
regulations must be observed when handling such substances, particularly the relevant national
accident prevention regulations.
Defective instruments are not to be disposed of as ordinary domestic waste. They should be
disposed of in accordance with the relevant regulations.
Samples should also be disposed of in compliance with the relevant legal requirements and
internal operating procedures.
Before setting up the instrument, check whether it is suitable for the available line voltage.
Always disconnect the instrument from the power outlet before opening it and changing the
fuses.
Only the specified fuses may be used.
The use of makeshift fuses and short-circuiting of the fuse holders are not permitted.
Controller EMS 3 supplies voltage to the mechanical / measuring stage S 150 x 100 mot. CAN.
The EMS
3 permits mains voltages in the 100 to 240 V ±10% 50/60 Hz range without
additional change in the voltage.
Operation of the instrument in explosive environments is not permissible.

INTRODUCTION Mechanical stage S mot. CAN
Carl Zeiss Notes on instrument safety Measuring stage S mot. CAN
8 435465-9000-701 08/2012
On microscopes with motorized focusing drive there is a
risk of pinching fingers
in the work
area when lowering the body of the microscope.
−Before
switching on check that the joystick on the SYCOP 3 is at the zero position and not
swiveled out, and that the foot pedal is clear.
−Automatic travel of the motorized tables can be interrupted by pressing the STOP button on
the focusing drive or the STOP button on the SYCOP 3, moving the joystick on the SYCOP 3
up and down, turning the knurled wheel on the HIP or pressing the Memory 1 or 2 keys on
the HIP.
−Do not reach into the possible travel path of the motorized stage. There is a risk of pinching
or injury to the fingers when the stage is positioned by the control unit.

Mechanical stage S mot. CAN INTRODUCTION
Measuring stage S mot. CAN Notes on warranty Carl Zeiss
08/2012 435465-9000-701 9
1.3 Notes on warranty
The manufacturer guarantees that the instrument is free of material or manufacturing defects when
delivered. Possible defects must be notified to us immediately and steps taken to minimize damage. If
notified of such a defect, the manufacturer is obligated to rectify it at his discretion, either by repairing
the instrument or delivering an intact replacement. No guarantee is provided for defects caused by
natural wear (wearing parts in particular) and improper use.
The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or
any other tampering with the instrument, particularly the removal or replacement of instrument
components, or the use of accessories from other manufacturers. Such actions will render any warranty
claims invalid.
With the exception of the work described in this operating manual, no maintenance or repair work is to
be carried out on these microscopes. Repairs may only be performed by Carl Zeiss service staff or
personnel specifically authorized by Carl Zeiss. In the event of a problem with the instrument, please
contact the Carl Zeiss microscopy service team or your local Carl Zeiss overseas representative.

DESCRIPTION Mechanical stage S mot. CAN
Carl Zeiss Indication for use Measuring stage S mot. CAN
10 435465-9000-701 08/2012
2DESCRIPTION
2.1 Indication for use
In conjunction with the selected microscope, the mechanical / measuring stage S mot. CAN is for motor-
supported XY-positioning of specimens in the beam path.
The mechanical / measuring stage S mot. CAN is intended for operation only with Axio Zoom.V.16
microscopes and SteREO Discovery.V20 / V12 / V8 stereomicroscopes.
When using the mechanical / measuring stage S mot. CAN the separate operating manuals for the Axio
Zoom.V16 or SteREO Discovery and, insofar as they are used, the
−Transillumination top 450 mot.
−SYCOP 3 system control panel
−and further components must be strictly observed.
2.2 Package check list
Name/Order No. Figure Remarks
Mechanical stage S
150x100 mot. CAN
435465-9000-000
or
Measuring stage S
150x100 mot. CAN
435465-9020-000
(without illustration)
CAN extension cable
HD15000000-0500-647
Connection between
EMS 3 and
mechanical / measuring
stage S 150x100 mot. CAN
Insert plate S. metal
237 x 157 mm
435465-9052-000
or
Insert plate S, glass
237 x 157 mm
435465-9053-000

Mechanical stage S mot. CAN DESCRIPTION
Measuring stage S mot. CAN Accessories and optional components Carl Zeiss
08/2012 435465-9000-701 11
2.3 Accessories and optional components
Name/Order No. Figure Remarks
EMS 3 controller
435610-9010-000
EMS 3
Required for operating
the stage
USB 2.0 cable to
workstation with
software
Country-specific power
cable
SYCOP 3 system control panel
435611-9010-000
SYCOP 3 for stage
control
Cable MDR 2x20
between SYCOP 3 and
EMS 3
Mobile coaxial drive XY CAN
435606-9000-000
Optionally for manual
control
Joystick XY; CAN (D)
432903-9010-000
Optionally for manual
control
Trackball XY; CAN (D)
432903-9000-000
Optionally for manual
control

DESCRIPTION Mechanical stage S mot. CAN
Carl Zeiss Microscope system with mechanical … Measuring stage S mot. CAN
12 435465-9000-701 08/2012
2.4 Microscope system with mechanical stage S 150x100 mot. CAN
Fig. 1 Axio Zoom.V16 with mechanical stage S
150x100 mot. CAN
Axio Zoom.V16 microscope
AMicroscope body (Axio Zoom.V16) with
control unit (HIP)
BBinocular photo tube
CEyepiece
DObjective
ENosepiece
FIntermediate tube (Fluar Illuminator Z mot.)
GCamera adapter with microscope camera
(AxioCam)
HReflected light illumination (slit-ring illuminator
with cold light source CL 9000 LED)
IUV/visual glare shield
KStand (focus motor with 490 mm profile column
and MaRC control unit on base plate 450)
LTransmitted light illuminator
(transillumination top 450 mot.)
MSpecimen stage (Mechanical stage S mot. CAN)
N SYCOP 3 system control panel
OEMS 3 controller
The items of microscope equipment shown here
are examples and may differ from those
actually provided!

Mechanical stage S mot. CAN DESCRIPTION
Measuring stage S mot. CAN Technical data Carl Zeiss
08/2012 435465-9000-701 13
2.5 Technical data
Dimensions
Mechanical stage / measuring stage S mot. CAN (length x width x height)............532 mm x 407 mm x 98 mm
Weight
Mechanical stage / measuring stage S mot. CAN............................................................................. 7.76 kg
Payload ....................................................................................................................................max. 3 kg
Power supply
Mechanical stage / measuring stage S mot. CAN................................................ viaCAN extension cable HD15
..........................................................................................................from Controller EMS 3 or focus motor
Travel speed
Via Joystick..........................................................................................................................max. 17 mm/s
Memory travels .................................................................................................................max. 100 mm/s
Ambient conditions
Storage (in packaging)
Permissible ambient temperature+5 to +40 °C
Permissible relative humidity max. 75 % at +35 °C (non-condensing)
Transport (in packaging):
Permissible ambient temperature........................................................................................... -40 to +70 °C
Operation
Permissible ambient temperature.......................................................................................... +10 to +40 °C
Permissible relative humidity max. 75%
Air pressure...............................................................................................................800 hPa to 1060 hPa
Degree of pollution2
EMS 3 controller
Operational area...................................................................................................................Closed rooms
Protection class I
Ingress protection rating.................................................................................................................... IP 20
Electrical safety conforms to DIN EN 61010-1 (IEC 61010-1)
and CSA and UL regulations
Overvoltage category .............................................................................................................................II
RFI suppression......................................................................................... conforming to EN 55011 Class A
Noise immunity........................................................................................... conforming to DIN EN 61326-1
Mains voltage range..............................................................................................100 to 240 V AC ± 10%
Mains frequency ..................................................................................................................... 50 to 60 Hz
Power consumption ..............................................................................................................max. 180 VA
Fuses ..............................................................................................................................2xT 4 A/H 205 V

SET-UP Mechanical stage S mot. CAN
Carl Zeiss General information Measuring stage S mot. CAN
14 435465-9000-701 08/2012
3SET-UP
3.1 General information
Read the Notes on instrument safety carefully prior to set-up and operation (see Section 1.2,
page 7).
The mechanical stage / measuring stage S mot. CAN is normally delivered together with the required
tools and optional accessories in several packages.
•Remove all components from the packaging and check that all components described on the delivery
note are present.
•Remove all shipping braces (adhesive tape or similar).
•Keep the original packaging for storage or for returning the instrument to the manufacturer, or
dispose of it properly.
3.2 Mounting the mechanical /
measuring stage S mot. CAN
In the event that a microscope system
is not yet available or assembled, the
microscope must initially be installed
and put into operation according to
the separate operating
manual. The
transillumination top 450 mot. may
already be mounted.
•Switch off the microscope and illumination.
•Remove the shipping screw (Fig. 2/2) on the
underside of the respective stage and keep it for
later use.
Only the measuring stage S mot. CAN
has a trigger socket (Fig. 2/1
) on its
underside.
Fig. 2 Removing the transport locking screw

Mechanical stage S mot. CAN SET-UP
Measuring stage S mot. CAN Mounting the mechanical / … Carl Zeiss
08/2012 435465-9000-701 15
3.2.1 Mounting the mechanical /
measuring stage S mot. CAN on
base 300 for epi-illumination
•If necessary, remove the metal plate (Fig. 3/1)
from base 300 (Fig. 3/2) by pressing and tilting
the rear edge.
•Set the mechanical / measuring stage (Fig. 3/4)
on the base 300 (stage drive pointing to the
left), align it to base 300 secure with the three
captive screws (Fig. 3/3) of the stage.
•Insert the metal plate (Fig. 3/5) into the stage.
−To do this, push the plate (Fig. 4/3) into the
stage (Fig. 4/1) with the left front corner
slightly inclined allow it to slide under slight
pressure against the springs (Fig. 4/4) until it
is completely inside the stage interface. The
plate is held in the stage by spring pressure.
Take care to ensure that the plate is correctly
positioned on the stage.
−To remove, insert a screwdriver into the
recess (Fig. 4/2) and carefully prise the plate
out.
The red dots on the stage (Fig. 3/4)
and metal plate (Fig. 3/5
) are to ensure
reproducibility of the alignment of the
me
tal plate and stage if the former
needs to be detached.
Fig. 4 Inserting the metal plate (glass plate) into or removing it from the stage
Fig. 3 Mounting the mechanical /
measuring stage mot. on base 300

SET-UP Mechanical stage S mot. CAN
Carl Zeiss Mounting the mechanical / … Measuring stage S mot. CAN
16 435465-9000-701 08/2012
3.2.2 Mounting the mechanical /
measuring stage S mot. CAN on
stand base 450 for epi-illumination
•If necessary, remove the metal plate (Fig. 5/1)
from insert plate (Fig. 5/3) by pressing and
tilting the rear edge.
If stand base 450 (Fig. 5/5) is not provided with an
adapter ring (Fig. 5/4) this must be mounted first:
−Remove the insert plate (Fig. 5/3). To do this,
remove the three screws (Fig. 5/2) and keep
them for possible later use.
−Insert the adapter ring (Fig. 5/4) into the
stand base 450 and secure with the three
captive screws (Fig. 5/6).
−Detach the insert plate (Fig. 5/3) from the
stand base 450 (Fig. 5/5).
•Set the mechanical / measuring stage (Fig. 6/3)
on the insert plate (Fig. 6/1) (stage drive
pointing to the left), align to the rear edge and
secure with the three captive screws (Fig. 6/2) of
the stage on the transillumination top.
•Insert the metal plate (Fig. 6/4) into the stage.
The procedure for inserting the plate
into and removing from the stage is
described on page 15.
The red dots on the stage (Fig. 6/3)
and metal plate (Fig. 6/4) are to ensure
reproducibility of the alignment of the
me
tal plate and stage if the former
needs to be detached.
Fig. 5 Mounting the adapter ring
Fig. 6 Mounting the mechanical /
measuring stage mot. on
stand base 450

Mechanical stage S mot. CAN SET-UP
Measuring stage S mot. CAN Mounting the mechanical / … Carl Zeiss
08/2012 435465-9000-701 17
3.2.3 Mounting the mechanical stage /
measuring stage S mot. CAN on the
transillumination base 300
•If necessary, remove the glass plate (Fig. 7/1)
from the transillumination base 300 (Fig. 7/2) by
pressing on the rear edge to tilt it.
•Insert stage adapter 84+120 (Fig. 7/3) into the
transillumination base 300 and screw it hand-
tight with the 4 captive screws.
•Set the mechanical / measuring stage (Fig. 7/5)
on the transillumination base 300 (stage drive
pointing to the left), align to the rear edge and
secure with the three captive screws (Fig. 7/4) of
the stage.
•Insert the glass plate (Fig. 7/6) into the stage.
The procedure for inserting the plate
into and removing from the stage is
described on page 15.
The red dots on the table (Fig. 7/5) and
metal plate (Fig. 7/6
) are to ensure
reproducibility of the alignment of the
metal plate and stage if the former
needs to be detached.
Fig. 7 Mounting the mechanical /
measuring stage mot. on
transillumination base 300

SET-UP Mechanical stage S mot. CAN
Carl Zeiss Mounting the mechanical / … Measuring stage S mot. CAN
18 435465-9000-701 08/2012
3.2.4 Mounting the mechanical /
measuring stage S mot. CAN on the
transillumination top DL 450 mot.
The procedure for mounting trans-
illumination top DL 450
mot. on base
plate
450 is described in the separate
operating manual of the trans-
illumination top.
•Remove the glass plate (Fig. 8/1) from insert
plate (Fig. 8/3) by pressing and tilting the rear
edge.
•Insert assembly aid d = 120 mm (Fig. 8/2) to
prevent the screws and small parts from being
lost in the transillumination top (Fig. 8/4).
•Remove the three short hexagon socket screws
(Fig. 8/5) on the insert plate and keep for
possible later use.
•Remove assembly aid d = 120 mm (Fig. 8/2)
again.
•Set the mechanical / measuring stage (Fig. 8/7)
on the insert plate (stage drive pointing to the
left), align to the rear edge of the
transillumination top (Fig. 8/4) and secure on
the transillumination top with the three captive
screws (Fig. 8/6) of the stage.
•Insert the glass plate (Fig. 8/8) into the stage.
The procedure fo
r inserting the plate
into and removing from the stage is
described on page 15.
The red dots on the table (Fig. 8/7) and
glass plate (Fig. 8/8
) are to ensure
reproducibility of the alignment of the
me
tal plate and stage if the former
needs to be detached.
Fig. 8 Mounting the mechanical /
measuring stage mot. on
transillumination top DL 450 mot.

Mechanical stage S mot. CAN SET-UP
Measuring stage S mot. CAN Connecting the mechanical / … Carl Zeiss
08/2012 435465-9000-701 19
3.3 Connecting the mechanical /
measuring stage S mot. CAN
Connecting to the microscope system
•Connect the stage directly to the corresponding
plug-in point CAN HD15 of controller EMS 3
with the CAN extension cable HD15 (Fig. 9/3)
via the upper connecting socket (Fig. 9/1).
•Connect SOCP 3 (Fig. 9/5) to EMS 3
•In addition to or as an alternative to SYCOP 3
the mobile coaxial drive XY CAN can be
connected using the latter's connecting cable
(Fig. 9/4) via the lower connecting socket
(Fig. 9/2) of the stage.
A device for transmitting a trigger
signal can be connected to the trigger
socket (Fig. 2/1
) of measuring
stage S mot. CAN.
•Connect EMS 3 to the mains supply using
power cable (Fig. 9/6).
Fig. 9 Connections on the mechanical /
measuring stage

Operation Mechanical stage S mot. CAN
Carl Zeiss Measuring stage S mot. CAN
20 435465-9000-701 08/2012
4OPERATION
Controller EMS 3 is indispensable for using the mechanical / measuring stage S mot. with SYCOP 3.
The stage function is controlled by the SYCOP 3 system control panel. See separate SYCOP 3 operating
manual
−for operation under:
Home\Microscope\Function\XYZ Position or
Home\Microscope\Function\XYZ Distance
−for hardware settings under:
Home\Microscope\Memory\Hardware Setting
The
Invert Direction
function can be used to reverse the direction of stage travel. See
SYCOP 3 operating manual under:
Home\Setup\Mode\Stage.
The Fast XY
function can be used for rapid stage movements over longer distances. To do
this, assign the Fast XY function to a button (see SYCOP 3 under: Home\Setup\Key
Settings\Buttons
) For quick positioning hold this button pressed while moving the joystick
accordingly.
If SYCOP 3 is not available on the microscope system, the mobile coaxial drive XY CAN can be
optionally used for external control of the stage (see Section 4.2).
The Parcentricity Manager can be activated on the SYCOP 3 for SteREO Discovery.V20 / V12
microscope systems with nosepiece S, coax 3x2 cod.
The Parcentricity Manager ensures that when the nosepiece is switched between the Stereo Mode (3D)
and Axial Mode (2D) positions the object detail being observed remains in the center of the image. The
necessary compensatory movement of the object is performed automatically by the installed mechanical /
measuring stage S mot. CAN.
Opening menus:
Home\Setup\Mode\Stage
•The function is activated / deactivated by
pressing the Compensate 2D/3D Changes
button.
•The LED lights up blue when the function is
activated.
The stage manager function is
automatically activated when using the
EMS
3. This ensures constant speed of
movement of object details in the
visual field.
Fig. 10 Activating/deactivating the
Parcentricity Manager