Zeiss Cell Observer SD User manual

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Carl Zeiss COPYRIGHT Cell Observer SD
2 423638-7044-001 M60-2-0021 e 01/2009
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the content of this manual and pay special attention to information
concerning safe operation of the Cell Observer SD.
Subject to alterations in the interest of technical progress; the manual is not covered by an update
service.
© Unless expressly authorized, forwarding and duplication of this document, as well as utilization and
communication of its content are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in case of patent granting or registration of a utility model.
Issued by: Carl Zeiss MicroImaging GmbH
P O Box 4041, D - 37030 Göttingen
Phone: [phone]
Fax: [phone]
E-mail: [email protected]
www.zeiss.de
Print no.: M60-2-0021 e
Date of issue: Version 6, 30.01.2009

Cell Observer SD CONTENTS Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 3
CONTENTS
Page
1Notes on Instrument Safety ..............................................................................................5
1.1 General Notes .......................................................................................................................5
1.2 Safety Instructions.................................................................................................................7
1.2.1 Notes on Laser Radiation.......................................................................................................7
1.2.2 Installation Instructions..........................................................................................................8
1.2.3 Service Interfaces ................................................................................................................11
1.2.4 Warning and Information Labels on the Instrument.............................................................13
1.3 Notes on Warranty..............................................................................................................19
2Instrument Description ....................................................................................................20
2.1 Intended Use.......................................................................................................................20
2.2 Functional Principle .............................................................................................................21
2.3 Instrument Description and Main Features...........................................................................22
2.3.1 Cell Observer SD .................................................................................................................23
2.3.2 CSU-X1 Control Box............................................................................................................25
2.3.3 Multi Laser Module .............................................................................................................26
2.3.4 Signal Distribution Box SVB 1 ..............................................................................................27
2.3.5 Filter Wheel.........................................................................................................................27
2.3.6 Basic Laser Safety Kit...........................................................................................................28
2.3.7 Safety Kit for Customer-Provided Laser Module...................................................................29
2.4 System Overview Cell Observer SD ......................................................................................30
2.5 Suitability of Objectives for the Use .....................................................................................31
2.6 Suitability of Optical Fibers for Use in Cell Observer SD........................................................31
2.7 Technical Data ....................................................................................................................32
3Start-Up.............................................................................................................................37
3.1 Installation Requirements ....................................................................................................37
3.1.1 Space Requirements............................................................................................................37
3.1.2 Power Requirements ...........................................................................................................38
3.1.3 Environmental Requirements...............................................................................................39
3.2 Requirements on Microscope Stand ....................................................................................40
3.3 Basic Microscope Settings ...................................................................................................41
3.4 Height Setting of CSU-X1 Spinning Disc Unit.......................................................................41
3.5 Mounting the Camera.........................................................................................................42
3.6 Connection of Spinning Disc Unit........................................................................................43
3.6.1 Connecting the CSU-X1M 1800/ 1800 Dual Cam Spinning Disc Unit...................................43
3.6.2 Connecting the CSU-X1M 5000/5000 Dual Cam or CSU-X1A 5000 Spinning Disc Unit .......43
3.6.3 Connecting the Filter Wheel................................................................................................44
3.7 Connecting the CSU-X1 Control Box...................................................................................44

Carl Zeiss TABLE OF CONTENTS Cell Observer SD
4 423638-7044-001 M60-2-0021 e 01/2009
3.8 Connecting the Distribution Box for Laser Safety .................................................................45
3.9 Coupling a Customer-Provided Laser to the Cell Observer SD System ..................................47
3.10 Safety Check before Laser Start-up...................................................................................... 48
3.11 Laser Safety Check ..............................................................................................................49
4Operation..........................................................................................................................50
4.1 Control and Functional Components...................................................................................50
4.1.1 CSU-X1 Spinning Disc Unit..................................................................................................50
4.1.2 CSU-X1 Control Box............................................................................................................ 51
4.1.3 Multi Laser Module .............................................................................................................51
4.2 Switching the System on ..................................................................................................... 52
4.2.1 Turning the CSU-X1 Spinning Disc Unit On ......................................................................... 52
4.2.2 Turning the Multiline Argon Laser On..................................................................................53
4.2.3 Turning the Multi Laser Module On..................................................................................... 54
4.3 Switching the System off..................................................................................................... 55
4.3.1 Turning the Cell Observer SD Off ........................................................................................55
4.3.2 Turning the Multiline Argon Laser Off .................................................................................55
4.3.3 Turning the Multi Laser Module Off ....................................................................................55
4.4 Operation Control of Spinning Disc Unit via AxioVision .......................................................56
4.5 Switching Between Camera Ports........................................................................................56
4.6 Exchange of Emission Filter .................................................................................................56
4.7 Exchange of Camera Adapter..............................................................................................57
4.8 Mounting the Filter Wheel ..................................................................................................58
4.9 Dual Cam Option................................................................................................................59
5Care, Maintenance, Troubleshooting and Service.........................................................60
5.1 Care....................................................................................................................................60
5.2 Maintenance.......................................................................................................................60
5.3 Troubleshooting.................................................................................................................. 61
5.4 Replacement of Fuses.......................................................................................................... 62
5.5 Replacement of HAL Halogen Lamp .................................................................................... 63
5.6 Connecting the TIRF Laser Module 2 to the Multi Laser Module ..........................................63
5.7 Laser Unit Retrofit ...............................................................................................................63
5.8 Requesting Service ..............................................................................................................64
6Appendix ..........................................................................................................................65
6.1 List of Abbreviations............................................................................................................65
6.2 Index...................................................................................................................................66

NOTES ON INSTRUMENT SAFETY
Cell Observer SD General Notes Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 5
1NOTES ON INSTRUMENT SAFETY
1.1 General Notes
The Cell Observer SD consists of the following two main components: CSU-X1 spinning disc unit and
Axio Observer.Z1. It has been designed and built to meet the requirements under DIN EN 61010-1
(IEC 61010-1) "Standard rules regarding the safety requirements for electrical equipment for
measurement, control, and laboratory use", DIN EN 60825-1 (IEC publication 60825-1) "Safety of laser
products" and DIN EN 61326-1 "Electrical equipment for measurement, control and laboratory use –
EMC requirements".
When combined with an Axio Observer.Z1, the CSU-X1 spinning disc unit complies with the require-
ments of Directive 98/79/EC (In Vitro Diagnostic Medical Device Directive). It carries the - mark.
The Cell Observer SD must be disposed in accordance with the WEEE Directive 2002/96/EC.
The following warning and information symbols are used throughout this Manual. They will help you to
promptly understand the type and purpose of advisory or alert notes contained herein.
NOTE
This symbol indicates a note that should be closely followed.
CAUTION
This symbol indicates a possible hazard to the instrument or system.
DANGER
This symbol indicates a possible hazard to the user of the instrument.
ACHTUNG
Disconnect power supply before any kind of intervention into the system!
DANGER
Laser radiation!
DANGER
Keep the laser zone clear of flammable and combustible materials.
DANGER
Dangerous electrical voltage!
Always bear in mind: The time you invest in making yourself familiar with the equipment will
pay off many times in your work.

NOTES ON INSTRUMENT SAFETY
Carl Zeiss General Notes Cell Observer SD
6 423638-7044-001 M60-2-0021 e 01/2009
In addition to this manual of the Cell Observer SD, please observe the information given in detail in the
following operating manuals:
−Operating Manual for Axio Observer Mikroscope (B 46-0111)
−Operating Manual AxioVision (B 48-0019 e)
−Operating Manual – Installation Manual for Peripherals (B 40-650 d)
−Operating Manual for computer system
−Operating Manual for Camera (AxioCam MRm, HSm, HRm or Photometrics EMCCD)
−Operating Manual for Confocal Scanner Unit CSU-X1 from Yokogawa (IM 85A07C25-01E)
−Operating Manual for CSUX1CU Control Unit from Yokogawa Electric Corporation
(IM 85A07C25-01E) , including accessories to CSUX1 (IM 85A07C26-01E and IM 85A07C27-01E)
−Operating Manual for Signal Distribution Box SVB1 (M60-2-0032)
−Operating Manual for Definite Focus (M60-2-0006)
−Operating Manual for HXP 120

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Safety Instructions Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 7
1.2 Safety Instructions
1.2.1 Notes on Laser Radiation
The Cell Observer SD relies on a class 3B laser device for operation and is labeled accordingly
(maximum 300 mW of output power). Laser instruments must be operated and handled in
accordance with the regulations concerning health and industrial labor protection. Furthermore,
the operator must observe all provisions of statutory law.
If operated conformingly, the Cell Observer SD does not create any risk of radiation exposure for
operating personnel. The following warning notes must be followed in all cases:
Inform your Laser Protection Officer before you begin with procedures for initial start-up of the
Cell Observer SD.
•Do not expose yourself to the laser beam!
•Do not introduce reflective objects into the beam path!
•Never open shielding or covers at the microscope system while the laser is operating!
•Never look – with or without optical aids – into the laser beam, not even in order to view a
specimen. Non-observance will cause risk of eye damage!
•Cover up unused positions of the lens turret and protect unused camera ports with dust
caps!
•Take appropriate protective measures if laser radiation causes the emergence of harmful
gases, dust and vapor or secondary radiation or explosive matter at the sample.
Keep the laser operating zone clear of flammable and combustible materials.
Additional national laser protection requirements must be duly observed!
Do not remove shielding from the fiber coupling and the joint between the microscope and the
CSU-X1 spinning disc unit.

NOTES ON INSTRUMENT SAFETY
Carl Zeiss Safety Instructions Cell Observer SD
8 423638-7044-001 M60-2-0021 e 01/2009
1.2.2 Installation Instructions
The Cell Observer SD system may only be installed or set-up by Carl Zeiss MicroImaging GmbH
Customer Service technicians or personnel specially authorized by the Manufacturer.
Carl Zeiss MicroImaging GmbH Customer Service will provide such services on a chargeable
basis.
Do not install the Cell Observer SD near heat sources, e.g. radiators or direct sunlight. To avoid
build-ups of heat, the ventilation slits at the microscope system must not be obstructed.
Do not operate the system, including standard-supplied accessories, in an explosive atmos-
phere, in the presence of such volatile anaesthetics or flammable solvents as alcohol, gasoline
or similar substances.
The main power plug may only be inserted into a socket with PE contact, and its built-in
protection device must not be rendered ineffective by using an extension cable without PE
conductor.
The Cell Observer SD components must be powered via two or three separate line power
cables, not via a common power distribution strip. This is necessary to prevent excessively
strong leakage currents (refer to section 3.1.2 on page 38).
The earth cable which is included in delivery (AWG10 green/yellow) must be used for
connecting the multi laser module to earth-ground. An earth-ground point must be available in
the installation room.
Check that your actual voltage levels correspond to the voltages that are indicated on the
nameplate of the laser module before you connect a line power cable.
For laser safety reasons, all TV outlets at the microscope must be connected to a camera or
sealed with a cap.
Maintenance or repair, including work for modification, removal or replacement of components
other than or in excess of the work scope described in this Operating Manual, as well as any
type of intervention into the inner equipment space may only be performed by technicians of
Carl Zeiss MicroImaging GmbH or persons expressly authorized to handle such work tasks on
behalf of Carl Zeiss MicroImaging GmbH. In particular, this applies to the microscope system,
the CSU-X1 spinning disc unit, the PC system and to cable connections.
Please note that the Cell Observer SD is an opto-electronic precision product. Improper
handling may easily affect its function or even damage the system. All claims for warranty will
be null and void in this case.

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Safety Instructions Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 9
On completion of work for installation or retrofitting of the Cell Observer SD System, an
authorized expert must perform a careful inspection to check for absence of defects. He/she
must also make sure that all laser protection shields are properly mounted.
Tube openings and other unused connection terminal must always be protected from dust and
moisture with appropriate sealing aids or anti-dust caps.
Do not operate the equipment delivered in potentially explosive atmospheres.
The Cell Observer SD is only approved for operation in closed rooms.
Establish a suitable workplace environment in order to prevent the formation of electrostatic
charges in electronic components.
Always disconnect the instrument from the power outlet before opening it and changing the
fuses.
Only use fuses appropriate for the rated current as specified. The use of makeshift fuses and
short-circuiting of the fuse holders are not permitted.
On completion of work for installation or conversion of the Cell Observer SD System, an
authorized expert must perform a careful inspection to check for proper working condition.
He/she must also make sure that all laser protection shields are correctly mounted.
Tube openings and other unused connection terminals must always be protected from dust and
moisture with appropriate sealing aids or anti-dust caps.
Dust and dirt may impair the instrument’s performance. The instrument must therefore be
protected as far as possible from such influences.
Clogged or covered ventilation slats may lead to heat build-up that will damage the instrument
and, in extreme cases, cause a fire. Always keep the ventilation slats clear and ensure that no
objects enter the instrument through the ventilation slats.
To prevent vibrations during Cell Observer SD operation, you should work with an Axio
Observer anti-vibration plate.

NOTES ON INSTRUMENT SAFETY
Carl Zeiss Safety Instructions Cell Observer SD
10 423638-7044-001 M60-2-0021 e 01/2009
If it is determined that protective measures are no longer effective, the instrument must be
switched off and secured against inadvertent operation. Please contact a Zeiss service agency or
the Carl Zeiss Microscopy Service to have the instrument repaired.
Read the Immersol 518 F® safety data sheet in all these cases.
Immersol 518 F®immersion oil causes skin irritation. Avoid any contact with your skin, eyes or
clothing.
In case of actual skin contact, wash off with plenty of water and soap. In case of eye contact,
rinse affected eye immediately with plenty of water for at least five minutes. See a specialized
physician in case of persisting irritation.
Proper disposal: Do not drain Immersol 518 F® into surface water or sewer system.
Avoid touching the hot lamp housing. Allow the instrument to cool down for at least
15 minutes.
The instruments are not equipped with any special devices for protection from corrosive,
potentially infectious, toxic, and radioactive or other substances that may be hazardous to
health. Make sure to observe all legal regulations when handling such substances, particularly
the relevant national accident prevention regulations.
Defective instruments must not be disposed of with household waste; they have to be disposed
of in compliance with the relevant legal provisions.
Samples have also to be disposed of properly according to the applicable legal provisions and
internal work instructions.

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Safety Instructions Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 11
1.2.3 Service Interfaces
Fig. 1-1 Service interfaces at the Axio Observer.Z1 – CSU-X1 spinning disc unit – multi laser module
CAUTION
The fiber connectors (Fig. 1-1/1and 2) may only be detached by Carl Zeiss service technicians.
If one fiber end is not firmly connected to the fiber mount, laser class 3B radiation will emerge!
Non-observance involves the risk of eye damage!
CAUTION
The cover (Fig. 1-1/3) at the joint between the CSU-X1 spinning disc unit and the
Axio Observer.Z1 may only be removed by Carl Zeiss service personnel.
If the CSU-X1 spinning disc unit and the Axio Observer.Z1 are not firmly connected with each
other, there may be emergence of class 3B laser radiation!
There may be eye damage if this rule is disregarded!

NOTES ON INSTRUMENT SAFETY
Carl Zeiss Safety Instructions Cell Observer SD
12 423638-7044-001 M60-2-0021 e 01/2009
Fig- 1-2 Service interface between transmitted light illumination carrier and HAL lamp
CAUTION
Only Carl Zeiss service personnel are allowed to release the clamping screw (Fig- 1-2/1).
If the lamp housing is removed, laser class 3B radiation will emerge!
Non-observance involves the risk of eye damage!
CAUTION
Switch the laser off in any case prior to replacing the halogen lamp of the HAL illuminator!
Non-observance involves the risk of eye damage!

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Safety Instructions Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 13
1.2.4 Warning and Information Labels on the Instrument
Follow all warning and information labels that are affixed to the Cell Observer SD and the Axio Observer
microscope. Please check that all labels shown below are actually in place.
Contact Carl Zeiss MicroImaging GmbH if a sign or label is found to be missing. You will receive
replacement free of charge.
Fig. 1-3 Warning and information labels at the Axio Observer.Z1 microscope

NOTES ON INSTRUMENT SAFETY
Carl Zeiss Safety Instructions Cell Observer SD
14 423638-7044-001 M60-2-0021 e 01/2009
Fig. 1-4 Warning and information labels at the Cell Observer SD

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Safety Instructions Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 15
Fig. 1-5 Warning and information labels at the filter wheel

NOTES ON INSTRUMENT SAFETY
Carl Zeiss Safety Instructions Cell Observer SD
16 423638-7044-001 M60-2-0021 e 01/2009
Caution!
Fuse in N-Line too.
Fig. 1-6 Warning and information labels at the multi laser module

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Safety Instructions Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 17
Fig. 1-7 Warning and information labels at the laser module, variant 1
Fig. 1-8 Warning and information labels at the laser module, variant 2

NOTES ON INSTRUMENT SAFETY
Carl Zeiss Safety Instructions Cell Observer SD
18 423638-7044-001 M60-2-0021 e 01/2009
Fig. 1-9 Warning and information labels at the SVB 1 (optional component)

NOTES ON INSTRUMENT SAFETY
Cell Observer SD Notes on Warranty Carl Zeiss
M60-2-0021 e 01/2009 423638-7044-001 19
1.3 Notes on Warranty
The manufacturer guarantees that the instrument is free from material and production defects when
delivered. Defects must be notified immediately on identification and maximum efforts must be
undertaken in order to keep the damage as small as possible. On receiving notice of such a defect, the
manufacturer will be under obligation to remove it, at its own option, either by providing adequate repair
services or by delivering a faultless product for replacement. No warranty is given for defects caused by
natural wear (wearing parts in particular) and improper use.
The manufacturer is not liable for damage resulting from faulty operation, negligence, or any other
meddling with the instrument, particularly the removal or replacement of instrument components or the
use of accessories from other manufacturers. Any such case will void the user’s warranty claims.
Except for action which is explicitly described in this operating manual, no maintenance or repair work to
the Cell Observer SD is allowed. Repairs may only be carried out by the Carl Zeiss Service or by persons
specifically authorized by it. Should any malfunction occur, please get in touch with your local Carl Zeiss
agency.
Customers using their own lasers will be solely responsible for overall laser safety of the Cell Observer SD
system. They shall also check for further required laser safety precautions and validity of all laser warning
labels that are affixed to the system, including the microscope.
The Manufacturer will refuse liability for modifications performed on the Cell Observer SD system.

INSTRUMENT DESCRIPTION
Carl Zeiss Intended Use Cell Observer SD
20 423638-7044-001 M60-2-0021 e 01/2009
2INSTRUMENT DESCRIPTION
2.1 Intended Use
Product name: CSU-X1M 1800 spinning disc unit
CSU-X1M 1800 Dual Cam spinning disc unit
CSU-X1M 5000 spinning disc unit
CSU-X1M 5000 Dual Cam spinning disc unit
CSU-X1A 5000 spinning disc unit
When configured with a CSU-X1 spinning disc unit, the Cell Observer SD provides a system to capture
confocal fluorescence pictures in real time. This is achieved with a multi-hole Nipkow scanning disc. A
multi-laser module is used as the light source. Alternatively, a multi-line Ar-laser may be selected.
One or two CCD cameras perform real-time image capturing.
The spinning disc unit should only be operated when combined with an Axio Observer.Z1 microscope
stand.
Human blood and tissue samples may be examined.
The laser modules (refer to configuration options, section 2.4 on page 30) may not be operated
on the Cell Observer SD, unless combined with a basic safety kit for Z1 "Axio Observer"
microscope stand (432927-9010-000).
Use the Cell Observer SD only with laser class 3B laser modules up to 300 mW of output
power.
Carl Zeiss MicroImaging GmbH cannot warrant the proper function of microscope system
components or the compliance with laser system safety requirements if lasers of a higher power
rating are selected.