Zeiss Axio Imager 2 User manual

Axio Imager 2
Upright microscope
Operating Manual

ZEISS Copyright / Trademarks Axio Imager 2
2 430000-7544-001 01/2016
Knowledge of this manual is required for operation of the device. You should therefore familiarize
yourself with its contents, paying particular attention to instructions concerning safe handling of the
device.
We reserve the right to make changes in the interest of technological advancement; the Operating
Manual is not subject to updating or revision.
© Unless expressly authorized, dissemination and duplication of this document and commercial
exploitation or communication of its contents are not permitted. Persons in contravention of this
copyright shall be liable for damages.
All rights reserved in the event of a patent granted or registration of a utility patent.
All names of companies and products mentioned in this manual may be trademarks or registered
trademarks. Third party products are cited for information purposes only. This does not represent
approval or recommendation of these products.
Carl Zeiss Microscopy AG accepts no liability for the performance or use of such products.
Issued by: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[e-mail]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Document number: 430000-7544-001
Date of issue: Version 10 – 13.01.2016

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CONTENTS
Page
1INTRODUCTION................................................................................................................ 7
1.1 Notes on device safety........................................................................................................ 7
1.2 Warning labels ................................................................................................................. 11
1.3 Warranty information ....................................................................................................... 13
1.4 Other operating manuals .................................................................................................. 13
1.5 Overall view of Axio Imager 2 - coded............................................................................... 14
1.6 Overall view of Axio Imager 2 - motorized......................................................................... 15
2INSTRUMENT DESCRIPTION........................................................................................... 16
2.1 Name and designated use................................................................................................. 16
2.2 Instrument description and main features.......................................................................... 16
2.3 Equipment and compatibility table .................................................................................... 18
2.4 System overview of Axio Imager 2..................................................................................... 20
2.5 Objectives ........................................................................................................................ 27
2.6 Eyepieces ......................................................................................................................... 28
2.7 Stage micrometers and eyepiece reticles............................................................................ 28
2.8 Technical data .................................................................................................................. 30
3FIRST-TIME SET-UP......................................................................................................... 33
3.1 Unpacking and installing the microscope........................................................................... 33
3.2 Installation conditions and notes ....................................................................................... 34
3.3 Attaching or changing binocular tube or phototube .......................................................... 35
3.4 Mounting the tube lens turret........................................................................................... 36
3.5 Inserting the eyepieces and auxiliary microscope................................................................ 36
3.5.1 Inserting the eyepiece reticles............................................................................................ 37
3.5.2 Compensation of ametropia when eyepiece reticles are used............................................. 37
3.5.3 Inserting the fold-over eyecups ......................................................................................... 37
3.6 Setting the interpupillary distance on the binocular tube.................................................... 38
3.7 Setting the viewing height ................................................................................................ 38
3.8 Connecting equipment to the camera port on the binocular phototube ............................. 39
3.9 Screwing in objectives....................................................................................................... 40
3.10 Inserting reflector turret, 6x20 compensator mount or 4-position modulator turret............. 40
3.11 Installing and removing P&C reflector modules.................................................................. 41
3.11.1 Installing a module ........................................................................................................... 41
3.11.2 Removing a module.......................................................................................................... 41
3.12 Changing the filter set in the reflector module FL P&C....................................................... 42
3.13 Changing the beam splitter in the reflector module FL P&C................................................ 43
3.14 Attaching or changing the condenser................................................................................ 45
3.15 Replacing the DIC prism on the universal condenser .......................................................... 46
3.16 Changing the DIC prism of the modulator turret (mot.) ..................................................... 47
3.17 Changing the stage carrier................................................................................................ 47
3.18 Setting stops for the adjustment range of the stage carrier dovetail guide .......................... 48
3.19 Setting the dovetail guide of the stage carrier.................................................................... 49

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3.20 Attaching the condenser carrier to the transmitted-light / reflected-light stage carrier..........50
3.21 Changing the fine-drive knob or the fine-drive disk on the stand ........................................50
3.22 Insert the filter in the double filter wheel............................................................................51
3.22.1 Double filter wheel, manual...............................................................................................51
3.22.2 Double filter wheel, motorized ..........................................................................................52
3.23 Axio Imager.Z2 / Z2m High-performance Focus ..................................................................53
3.23.1 Activating the high-performance focus ..............................................................................53
3.23.2 Deactivating the high-performance focus...........................................................................54
3.24 Mounting the TFT display on the motorized stand ..............................................................54
3.25 Attaching the TFT display to the docking station ................................................................55
3.26 Installing the focus linear sensor ........................................................................................56
3.27 Assembling the multidiscussion equipment ........................................................................56
3.28 Connecting to power ........................................................................................................57
3.28.1 Coded stand .....................................................................................................................57
3.28.2 Motorized stand................................................................................................................57
3.29 HAL 100 halogen illuminator .............................................................................................58
3.29.1 Attaching the HAL 100 halogen illuminator .......................................................................58
3.29.2 Separate power supply of the HAL 100 halogen illuminator for reflected light.....................59
3.29.3 Adjusting the HAL 100 halogen illuminator........................................................................60
3.29.4 Replacing the HAL 100 halogen lamp ................................................................................61
3.30 Installing the LED illuminator for transmitted light ..............................................................62
3.31 VIS-LED attachment lamp for transmitted light...................................................................64
3.31.1 The VIS-LED attachment lamp is only intended for use with transmitted light. .....................64
3.31.2 Adjusting the VIS-LED attachment lamp.............................................................................65
3.32 Mounting the microLED attachment lamp for transmitted light...........................................65
3.33 HBO 100 illuminator..........................................................................................................66
3.33.1 Inserting the HBO 103 W/2 mercury vapor short-arc lamp ..................................................66
3.33.2 Attaching the HBO 100 illuminator....................................................................................66
3.33.3 Aligning the HBO 100 illuminator ......................................................................................67
3.33.4 Setting up the switching mirror for two illuminators (manual or motorized) ........................68
3.34 Electrical connections on the rear of the microscope...........................................................69
3.34.1 Coded stand .....................................................................................................................69
3.34.2 Motorized stand................................................................................................................69
3.35 Mount sample stage..........................................................................................................70
3.35.1 Fixed mechanical stage 75x50 R ........................................................................................70
3.35.2 Rotatable mechanical stage 75x50/240° R..........................................................................71
3.35.3 Adjusting the stage drive length on the ergonomic drive ....................................................72
3.35.4 Removing and attaching additional sleeves ........................................................................73
3.35.5 Adjusting the action (torque) of the two drive knobs of the ergonomic drive.......................73
3.35.6 Changing the specimen holder ..........................................................................................74
3.36 Rotary stage Pol ................................................................................................................74
3.36.1 Removing the rotary stage Pol ...........................................................................................74
3.36.2 Attaching rotary stage Pol .................................................................................................75
3.36.3 Centering rotary stage Pol .................................................................................................75
3.36.4 Centering objectives..........................................................................................................76
3.37 Mechanical stage 75x50 mot. CAN....................................................................................77
3.37.1 Assembling and connecting 75x50 mot. CAN mechanical stage with coaxial drive ..............77

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3.37.2 Assembling mechanical stage 75 x 50 mot. CAN and connecting joystick / trackball ........... 79
3.37.3 Mounting the specimen holder on the mechanical stage.................................................... 80
3.37.4 CAN/USB converter .......................................................................................................... 80
3.37.5 Docking station ................................................................................................................ 81
3.38 Installing Polarizer D, fixed ................................................................................................ 82
4OPERATION .................................................................................................................... 83
4.1 Coded Axio Imager 2, coded, operation and function controls ........................................... 83
4.2 Axio Imager 2 operation and function controls (motorized version) .................................... 89
4.3 Switching the microscope and HAL 100 illuminator on/off ................................................. 94
4.3.1 Switching on .................................................................................................................... 94
4.3.2 Switching off.................................................................................................................... 94
4.4 Switching the HBO 100 on/off .......................................................................................... 95
4.5 Binocular phototube 30°/25 mot. with two camera ports (2TV tube mot.).......................... 95
4.6 The Light Manager ........................................................................................................... 97
4.6.1 Light Manager Mode: OFF ................................................................................................ 98
4.6.2 Light Manager Mode: CLASSIC ......................................................................................... 98
4.6.3 Light Manager Mode: SMART ........................................................................................... 99
4.6.4 Light Manager of the coded microscope.......................................................................... 100
4.6.5 Light Manager and Dazzle Protection control on manual stands....................................... 100
4.6.6 Light Manager of motorized microscope ......................................................................... 101
4.7 The Contrast Manager.................................................................................................... 102
4.8 Operating the motorized microscope via the touchscreen of the TFT display ..................... 103
4.8.1 Screen layout ................................................................................................................. 104
4.8.2 Menu structure............................................................................................................... 107
4.8.3 Home page .................................................................................................................... 108
4.8.4 Favorite page ................................................................................................................. 111
4.8.5 Microscope page............................................................................................................ 111
4.8.6 Config page ................................................................................................................... 126
4.8.7 User page....................................................................................................................... 145
4.8.8 Display page................................................................................................................... 153
4.9 Capturing large overviews (tiles or MosaiX images) on Axio Imager 2............................... 154
4.10 Relocate an event on a sample carrier ............................................................................. 156
4.10.1 Microscope equipment and basic settings........................................................................ 156
4.10.2 Calibration of the stage .................................................................................................. 156
4.11 Automatic component recognition and user-defined component equipment.................... 157
4.11.1 Programming of rewritable ACR reflector modules .......................................................... 158
4.11.2 Configuration of user-defined filter wheels...................................................................... 159
4.11.3 Configuration of motorized condensers .......................................................................... 160
4.12 Illumination and contrast methods .................................................................................. 161
4.12.1 Setting transmitted-light brightfield according to KÖHLER ............................................... 161
4.12.2 Setting the transmitted-light darkfield ............................................................................. 164
4.12.3 Setting transmitted-light phase contrast .......................................................................... 167
4.12.4 Setting transmitted-light differential interference contrast (DIC) ....................................... 169
4.12.5 Setting transmitted-light polarization for orthoscopic observation .................................... 172
4.12.6 Setting transmitted-light polarization for conoscopic observation – determining the
optical character of crystals............................................................................................. 180

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4.12.7 Setting reflected-light brightfield .....................................................................................183
4.12.8 Setting reflected-light darkfield........................................................................................186
4.12.9 Setting reflected-light DIC and reflected-light C-DIC........................................................187
4.12.10 Setting reflected-light TIC...............................................................................................189
4.12.11 Setting reflected light fluorescence ..................................................................................192
4.12.12 Setting reflected-light polarization – Detection of bireflection and reflection pleochroism ..194
5CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........................................196
5.1 Instrument care...............................................................................................................196
5.2 Instrument maintenance..................................................................................................197
5.2.1 Performing checks...........................................................................................................197
5.2.2 Changing fuses on the manual stand...............................................................................197
5.2.3 Changing fuses on the VP232-2 CAN power supply ........................................................198
5.3 Troubleshooting..............................................................................................................199
5.4 Spares, consumables and tools ........................................................................................202
5.5 Requesting service...........................................................................................................203
6Appendix ......................................................................................................................204
6.1 List of abbreviations ........................................................................................................204
6.2 Index ..............................................................................................................................206
6.3 Industrial property rights .................................................................................................210

Axio Imager 2 INTRODUCTION ZEISS
Notes on device safety
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1INTRODUCTION
1.1 Notes on device safety
The Axio Imager 2 microscopes have been designed, produced and tested in compliance with
DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety requirements for electrical equipment for
measurement, control, and laboratory use".
The devices meet the requirements of the European Directive IVDD 98/79/EC (In Vitro Diagnostic Medical
Devices) and RoHS Directive 2011/65/EC and carry the mark.
The devices are disposed of in accordance with WEEE Directive 2012/19/EC.
This Operating Manual contains information and warnings that must be followed by the owner/operator
personnel.
The following warning and information symbols are used in this Operating Manual:
NOTE
This symbol indicates an instruction which requires particular attention.
ATTENTION
This symbol indicates a potential hazard to the instrument or system.
CAUTION
This symbol indicates a potential hazard to the user.
CAUTION
Hot surface!
CAUTION
Emission of UV radiation!
CAUTION
LED radiation in 400 nm to 700 nm range! LED risk group 1 in accordance with DIN EN
62471:2009
Do not look into the LED light!
CAUTION
Disconnect the instrument from the power supply before opening!
CAUTION
Risk of crushing!
ATTENTION
Stand-
by: Switching the Axio Imager.Z2 and Z2m off using the standby switch only turns off
the internal computer. The mains supply is not switched off.

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Notes on device safety
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The Axio Imager 2 microscopes and their original accessories are to be used only for the microscopy
procedures described in this Operating Manual.
Particular attention must be paid to the following instructions:
The manufacturer cannot
assume any liability for any other applications of the instrument,
including the use of individual modules or components. This also applies to all service or repair
work that is not carried out by authorized service personnel. Failure to comply with this shall
render all warranty claims invalid.
The power plugs may only be connected to sockets with an earth contact. The protective
capacity must not be rendered ineffective by the use of extension cables with no grounding
conductor.
If protection
measures no longer prove effective, the instrument must be switched off and
safeguarded against inadvertent operation. Please contact ZEISS Service or the Carl Zeiss
Microscopy Service to repair the instrument.
On stands with motorized focusing drive, there is the risk of fingers being crushed between the
stage carrier and the base of the stand when the stage is lowered. Do not, therefore, place your
hands under the stage carrier.
The manual microscopes (Axio Imager.A2, .A2 LED, .A2m, .D2 and .D2m) have a power supply
integrated in the stand which allows line voltages to be used in the ranges 100 to 127 V and
200 to 240 V AC ±10 %, 50 Hz – 60 Hz, without the voltage setting on the instrument having
to be changed.
The motorized models (Axio Imager.M2, .M2m, .Z2 and .Z2m) are powered by the separate
power supply VP232-2 belonging to the stand. The voltage setting need not be changed in the
line voltage range 100 to 240 V ±10 %, 50 Hz – 60 Hz on this power supply, either.
The HBO 100 (ebq 100 dc) and XBO 75 (ebx 75
isolated) ballast units are designed for a line
voltage range from 100 to 240 V AC, 50 Hz – 60 Hz
, and automatically adapt to the supplied
line voltage.
Before switching on the instrument, check whether suitable line voltage is available.
Disconnect the device from the mains power supply before gaining access to the interior of the
device or changing the fuse. See Section 5.2.2.
Only use fuses specified in the information given in the Technical Data. Use of makeshift fuses
and short-circuiting of the fuse holders are not permitted.
The Axio
Imager microscopes are not equipped with any special devices for protection from
substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that
could be hazardous to health. Observe all legal regulations, parti
cularly the relevant national
accident prevention regulations when handling such substances.

Axio Imager 2 INTRODUCTION ZEISS
Notes on device safety
01/2016 430000-7544-001 9
Operation of the devices in explosive environments is not permissible. Operation of the devices
is only permitted in closed rooms.
Gas discharge lamps, e.g. HBO 50; HBO 100 or XBO
75, emit ultraviolet radiation, which can
cause burns to the eyes and skin. Therefore, never look directly into the light of these lamps
and avoid direct, unprotected incidence of their light on your skin. When using the microscope,
always use the protective devices belonging to the instrument (e.g. special attenuation filters or
the fluorescence shield). When they are hot, gas discharge lamps are under high internal
pressure. Therefore, change them only when they have cooled down, and make sure protective
gloves and a face guard are worn.
When fluorescence filters are used, the heat protection filter for heat emitted by the
microscope illuminator must not be removed, since fluorescence filters are sensitive to heat and
their performance could be impaired.
Avoid touching the hot lamp housing. Always disconnect the power plug before changing the
lamps and allow the instrument to cool down for approx. 15 minutes.
Dust and dirt may impair the instrument’
s performance. The instrument must be effectively
protected from such influences and covered with the dust cover when not in use. Always check
whether the instrument is switched off before you cover it.
Clogged or covered ventilation slots may lead to heat build-up that will damage the device and,
in extreme cases, cause a fire. Always keep the ventilation slots clear and ensure that no objects
enter or fall into the instrument through the ventilation slots.
The instruments may only be operated by trained personnel who must be aware of the possible
dangers involved with microscopy and the particular application concerned. The Axio Imager
microscopes are high-
precision instruments that can be impaired in their performance or
destroyed if handled improperly.
The LED illuminator is a class 2M device. Do not look directly into the LED light.
Do not dispose of defective instruments in regular domestic waste; these should be disposed of
in accordance with prevailing legal requirements.
Specimens should also be disposed of in compliance with the prevailing legal requirements and
internal operating procedures.

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Notes on device safety
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For stands equipped with motorized focusing drives, there is a risk of fingers being crushed
in the working area when the microscope stage is lowered.
−
Do not reach into the area between the microscope stage / stage carrier / condenser
carrier and the stand base while the stage is being lowered.
−
The automatic movement can be stopped by pressing the STOP button on the TFT
display. (See Fig. 2).
When a scanning stage (approx. weight: 4 kg) is used, the high-
performance focus must be
activated to protect the focusing drive (see page 53
). Sample weights of up to 5 kg can be
placed on the stage if the high-performance focus is activated.
It is essential to read the safety data sheet provided with Immersol 518 Fimmersion oil.
Immersol 518 Fimmersion oil is a skin irritant. Avoid contact with skin, eyes and clothing.
In the event of contact with the skin, rinse with plenty of water and soap.
In the event of contact with the eyes, rinse the eyes
immediately with plenty of water for at
least five minutes. If irritation persists, consult a physician.
Correct disposal of Immersol 518 Fimmersion oil. Ensure that the immersion oil does not enter
surface water or the sewage system.
Do not
replace detachable power cables with power cables that do not meet the specifications.
Only the specified power cables should be used.
The Axio Imager.Z2 or Z2m can only be disconnected from the mains supply using the on/off
switch on the external VP232-2 power unit.

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Warning labels
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1.2 Warning labels
Fig. 1 "Crushing Hazard" label warning on
the base of motorized stands
Fig. 2 STOP button
The STOP button on the TFT display switches off the movement of the focusing drive instantaneously to
prevent a collision with the stage or the specimen placed on it.
Actuation of the STOP button halts the movement of the focusing drive immediately.
Fig. 3 Warning labels on the underside of the LED illuminator

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Warning labels
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Fig. 4 Warning labels on the VIS-LED attachment lamp
Fig. 5 Warning labels on the underside of the microLED

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Warranty information
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1.3 Warranty information
The manufacturer guarantees that the instrument is free of material or manufacturing defects upon
delivery. Any defects must be reported immediately and steps taken to minimize damage. If such a defect
is reported, the instrument manufacturer shall be obliged to correct the fault, either by repairing the
instrument or replacing it with a new one, at the manufacturer's discretion. No warranty is given for
defects caused by natural wear and tear (particularly of wearing parts) and improper use of the
instrument.
The instrument manufacturer shall not be liable for damage caused by misuse, negligence or any other
tampering with the instrument, particularly the removal or replacement of instrument components, or
the use of accessories from other manufacturers. Such actions shall invalidate any warranty claims.
With the exception of the work described in this Operating Manual, no maintenance or repair work is to
be carried out on the Axio Imager 2. Repairs may only be performed by ZEISS Service or individuals
specially authorized by ZEISS Service. In the event of a problem with the instrument, please contact your
local ZEISS representative.
1.4 Other operating manuals
In addition to the operating manual for the Axio imager 2, the following operating manuals should also
be consulted:
−Axio Observer Microscope Operating Manual
−ZEN Software Description (blue edition) (online version)
−Operating manual: Installation handbook for peripheral equipment
−Operating manual for the computer system
−Operating manual for the reflected light fluorescence illuminator HBO 100
−Operating manual for HXP 120 V
−Operating manual for the control computer
−Operating manual for the monitor
−Operating manuals for each incubation system
−Operating manual for the SVB 1 signal distribution box
−Operating manual for Auto Focus
−Operating manual for multidiscussion equipment

ZEISS INTRODUCTION Axio Imager 2
Overall view of Axio Imager 2 - coded
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1.5 Overall view of Axio Imager 2 - coded
1Eyepieces
2Binocular tube
3Reflected-light illuminator (HBO 100)
4Microscope stand, manual
5Transmitted-light illuminator (HAL 100)
6Mechanical stage
7Nosepiece
8Condenser
9Reflector turret
Fig. 6 Overall view of Axio Imager 2 - coded

Axio Imager 2 INTRODUCTION ZEISS
Overall view of Axio Imager 2 - motorized
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1.6 Overall view of Axio Imager 2 - motorized
1Eyepieces
2Binocular phototube
3Reflected-light illuminator (HBO 100)
4Microscope stand, motorized
5Transmitted-light illuminator (HAL 100)
6TFT display
7Mechanical stage
8Nosepiece
9Condenser
10 Reflector turret
Fig. 7 Overall view of Axio Imager 2 - motorized

ZEISS INSTRUMENT DESCRIPTION Axio Imager 2
Name and designated use
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2INSTRUMENT DESCRIPTION
2.1 Name and designated use
Manufacturer's designation: Axio Imager: Axio Imager MAT: Axio Imager LED:
Axio Imager.A2 Axio Imager.A2m Axio Imager.A2 LED
Axio Imager.D2 Axio Imager.D2m
Axio Imager.M2 Axio Imager.M2m
Axio Imager.M2p Axio Imager.Z2m
Axio Imager.Z2
The Axio Imager / Axio Imager MAT microscopes have been designed as universal microscopes for
applications in biology and medicine for the examination of blood and/or tissue specimens from the
human body, as well as for materials examinations.
They may also be used as true reflected-light microscopes or, if equipped with transmitted-light
equipment, as combined reflected-light/transmitted-light microscopes.
Typical biomedical applications of the Axio Imager 2 microscopes include:
−Medical examinations in laboratories (research), clinics and medical practices
−Science and research (colleges, universities) in the fields of medicine and biology
−Industrial applications (pharmacology, food technology)
Typical applications of the Axio Imager MAT microscopes include:
−Metallographic laboratories
−Automotive industry
−Microsystems engineering
2.2 Instrument description and main features
With its advanced pyramid and modular design, the Axio Imager microscope incorporates time-tested
principles in microscope construction, combining modern requirements in design, ergonomics, operating
convenience and function with technical performance.
Depending on the instrument configuration, the following microscopy and contrasting techniques are
possible:
Transmitted light:
Reflected light:
- Brightfield (H)
- Darkfield (D)
- Phase contrast (Ph)
- Differential Interference Contrast (DIC)
- Polarization contrast (Pol)
- Circular polarization
- Brightfield (H)
- Darkfield (D)
- Differential Interference Contrast (DIC)
- Differential Interference Contrast in circularly
polarized light (C-DIC)
- Polarization contrast (Pol)
- Fluorescence

Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS
Instrument description and main features
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The Axio Imager 2 microscope is available in ten different stand versions. However, the scope of the
equipment of these stands is variable and can be customized to the user's requirements within the range
of optional microscope components.
Coded models:
Axio Imager.A2, A2m and A2 LED
Partially motorized models:
Axio Imager.D2, D2m
Motorized models:
Axio Imager.M2, M2m, M2p
Axio Imager.Z2 and Z2m
The binocular phototubes and suitable adapters permit one microscope camera, one reflex camera or one
digital / video camera to be attached for documentation purposes.

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Equipment and compatibility table
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2.3 Equipment and compatibility table
Component Option / comment
Stand
A2
LED A2 M2p M2 D2 Z2 A2m M2m D2m Z2m
Microscope stand coded + + - - + - + - + -
motorized - - + + O* + - + O* +
Coding PC readable + + + + + + + + + +
Tube lens turret coded O O O O O O O O O O
motorized - - O O - O - O - O
Reflector turret
6 positions, coded O O O O O O O - O O
6 positions, motorized - - O O O O - + O O
6x motorized HD - - - - - O - - - O
10x motorized ACR** - - - - O O - - O O
Objective nosepiece
6x coded POL O O - O O O O O O O
6x coded HD DIC O O - O O O O O O O
6x motorized HD DIC - - - O - O - O - O
6x motorized HD DIC ACR - - - O - O - O - O
7x coded HD O O + O O O O O O O
7x motorized HD - - - O - O - O - O
Modulator turret for
C-DIC / TIC
manual O O O O O O O O O O
motorized**** - - - O - O - O - O
Modulator turret for
transmitted light - DIC motorized**** - - - - - O - - - O
Attachable stage carrier with
condenser carrier
0 mm – 25 mm + + + + + O O O O O
Attachable stage carrier for
detachable condenser carrier
0 mm – 45 mm
O O O O O O O O O O
Attachable reflected-light
stage carrier 0 mm – 63 mm - O - O O O O O O O
Transmitted-light illumination manual - + - - + - O O O O
motorized - - - + - + - - - O
LED transmitted light on
condenser carrier - + O + O O O O O O O
Transmitted light double filter
wheel
manual - + - O O O O O O O
motorized - - - O - O - - - O
Reflected-light illumination manual*** O O O O O O + - + -
motorized*** - - - - - O - + - +
Reflected-light luminous-field
diaphragm
manual O O O O O O + O + O
motorized - - - - - O - O - O

Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS
Equipment and compatibility table
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Component Option / comment
Stand
A2
LED A2 M2p M2 D2 Z2 A2m M2m D2m
Z2m
Reflected-light aperture
diaphragm slider
manual O O O O O O O O O O
motorized - - - - - O - O - O
Reflected light double filter
wheel
manual O O O O O O O O O O
motorized - - - - - O - O - O
FL Attenuator manual O O O O O O O O O O
motorized - - - - - O - O - O
Reflected light /
transmitted light selection
manual + + - - + - + - + -
Software - - + + - + - + - +
Mixed light
with extra power supply
external power supply + + - - + - + - + -
external power supply,
CAN - - + + - + - + - +
Focus (z-axis)
manual + + - - + - + - + -
motorized, 25 nm - - + + - - - + - -
High-performance focus
(motorized, 10 nm) - - - - - + - - - +
Focus linear sensor - O O O O O
Auto Focus - / 6) O O O O O
TFT display - - - O + - + - + - +
ApoTome - O O O O O O O O O O
Power supply External - - + + - + - + - +
Internal + + - - + - + - + -
Mechanical CAN stages motorized**** O O O O O O O O O O
Scanning stages Piezo O O O O O O O O O O
DC / stepper motors O O O O O O O O O O
Fast z-piezo operation With manual stage O O O O O O O O O O
With scanning stage O O O O O O O O O O
Motorized 2TV tube - - - O O - O - O - O
Condensers manual O O O O O O O O O O
motorized - - O O - O - O - O
+ = Included in stand
O = Optional
- = Not possible
* = Motorized (6-position and 10-position) reflector turret may be used.
** = ACR function not available with Axio Imager D2 and D2m.
*** = All reflected-light illumination systems come with a motorized shutter.
For fluorescence applications, this may be replaced optionally by a high-speed shutter.
**** = USB/CAN converter 432909 is required for use on Axio Imager A2 LED, A2, A2m, D2 and D2m.
***** = Motorized only if used with an objective nosepiece.
6) = Sideways camera path deflection is used, analyzer slider plane cannot be used for slider.

ZEISS INSTRUMENT DESCRIPTION Axio Imager 2
System overview of Axio Imager 2
20 430000-7544-001 01/2016
2.4 System overview of Axio Imager 2
Condensers