Zeiss LSM 880 User manual

ZEISS LSM 880 INVERTED (i880) QUICK START GUIDE
The Zeiss LSM880 is a laser scanning confocal microscope with full confocal and multiphoton capability. The
confocal detectors are spectrally resolved and can be used with either the standard (single photon) excitation
lines or the multiphoton excitation lines. The non-descanned (NDD) detectors can only be used with multiphoton
excitation.
INSTRUMENT OVERVIEW
AND USER RESOURCES
FULL TURN ON SEQUENCE (FIRST ONE ON)
1) If the computer is off, turn it on and let it boot.
2) Turn on the main switch.
3) Turn on the Systems/PC switch. (When
touchscreen has booted, adjust heating and
CO2 under Microscope->Inkubation if
applicable.)
4) Turn on the Components switch. Wait for the
RTC to boot (see under table).
5) Log in to the computer.
6) Start Zen and select Start System Button.
7) Turn on laser lines you will be using. (If you are
using multiphoton excitation, turn on both lines
on even if you are just using one. Note that the
Ar and multiphoton lines take a while to turn on.
You can follow the warm-up progress by
selecting the laser and the Laser Properties
below)
FULL TURN OFF SEQUENCE (LAST ONE ON)
9) Save your data and transfer it to the fileshare.
8) Remove your sample and clean off your
objective. (If using incubation, turn temp and
CO2 regulation off and close CO2 tank using
main gray valve only.)
7) Turn off lasers.
6) Exit Zen.
5) Log out of the computer.
4) Turn off the Components switch.
3) Turn off the Systems/PC switch.
2) Wait a few minutes for the Ar laser to cool.
Turn off the main switch.
1) Do not turn off computer.
PARTIAL TURN OFF SEQUENCE
(Someone else is scheduled after your time slot)
9) Save your data and transfer it to the fileshare.
8) Remove your sample and clean off your
objective. (If using incubation, turn temp and
CO2 regulation off and close CO2 tank using
gray handle only.)
7) Turn off lasers.
6) Exit Zen.
5) Log out of the computer.
PARTIAL TURN ON SEQUENCE
(Microscope already on)
5) Log in to the computer.
6) Start Zen and select Start System Button.
7) Turn on laser lines you will be using. (If you are
using multiphoton excitation, turn on both lines
on even if you are just using one. Note that the
Ar and multiphoton lines take a while to turn on.
You can follow the warm-up progress by
selecting the laser and the Laser Properties
below)

LASER LINES
STANDARD OBJECTIVE SPECIFICATIONS
Mag
NA
Immersion
medium
CS thickness
(mm)
WD (mm)
Objective Type
Trans > 0.5
10X
0.3
Air
0.17
5.2
EC Plan-Neofluar
350-1200nm
20X
0.5
Air
0.17
2.0
EC Plan-Neofluar
350-1250nm
32X
0.85
Water
0-0.17
1.1
C-Achroplan
450-1300nm
40X
1.1
Water
0.14-0.19
0.62
LD C-Apochromat
350-1150nm
40X
1.4
Oil
0.17
0.13
Plan-Apochromat
380-1200nm
10X
0.45
Water
0.17
1.8
C-Apochromat
350-1200nm
63X
0.75
Air
0-1.5
1.7
(at 0.75 CS)
LD Plan-Neofluar
350-950nm
NDD DETECTOR FILTERS
*Do not use any laser lines <700nm with the NDD detectors!