Zeiss Axioscope 7 User manual

Operating Manual
Axioscope 5/7/Vario
Upright Microscope for Routine and Entry-level Research
Microscope for Routine and Entry-Level Research

ZEISS Copyright / Trademarks Axioscope 5/7/Vario
2 430035-7344-001 03/2018
Knowledge of this manual is essential for the operation of the instrument. Please familiarize yourself with
the contents of this manual and pay special attention to instructions concerning safe operation of the
instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, dissemination and duplication of this document as well as
commercial exploitation and communication of its contents are not permitted. Persons in
contravention of this copyright are liable to pay compensation for damages.
All rights reserved, including rights created by patent grant or registration of a utility model.
All names of companies and products mentioned in this manual may be trademarks or registered
trademarks. Third party products are cited for information purposes only and this does not represent
approval or recommendation of these products.
Carl Zeiss Microscopy GmbH assumes no liability for the performance or use of such products.
Published by Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[e-mail]
www.zeiss.com/microscopy
Carl Zeiss Suzhou Co., Ltd.
Modern Industrial Square 3-B, No.333
XingPu Road SIP
215126 Suzhou, China
Order No.: 430035-7344-001
Issue date: Version 3 – 15 March 2018
authorized dealer:
Pulch + Lorenz microscopy
Am Untergrün 23, -79232 March
tel: [phone]
fax: [phone]
mail: [email protected]
web: pulchlorenz.de

Axioscope 5/7/Vario Contents / List of Illustrations ZEISS
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CONTENTS
Page
1INTRODUCTION ................................................................................................... 9
1.1 Notes on instrument safety .......................................................................................... 9
1.2 Warning labels on the microscopes............................................................................ 13
1.3 Notes on the warranty................................................................................................ 16
2DESCRIPTION OF THE INSTRUMENT................................................................. 17
2.1 Intended use................................................................................................................ 17
2.2 Technical data.............................................................................................................. 18
2.3 Interface diagram........................................................................................................ 20
2.4 Controls and functional elements on the microscope............................................... 21
2.4.1 Stand models................................................................................................................. 21
2.4.2 Axioscope 5 stands, Bio-TL ............................................................................................. 22
2.4.3 Axioscope 5 stand, Bio-TL/RL .......................................................................................... 24
2.4.4 Axioscope 5 stand, Pol-TL/RL .......................................................................................... 26
2.4.5 Axioscope 5 stand, Mat-TL/RL......................................................................................... 28
2.4.6 Axioscope 7 stand, Mat-TL/RL mot.................................................................................. 30
2.4.7 Axioscope 5 Vario material stand.................................................................................... 32
2.4.8 Functions of stands keys and display elements................................................................. 34
2.5 Controls and functional elements on microscope components ................................. 36
2.5.1 Binocular photo tube 30°/23 (100:0/0:100)..................................................................... 36
2.5.2 Binocular ergo tube/ergo photo tube 20°/23 and ergo photo tube 15°/23, each with
continuous vertical adjustment ....................................................................................... 37
2.5.3 Nosepiece with objectives............................................................................................... 38
2.5.4 Mechanical stage 75x50................................................................................................. 38
2.5.5 Low-power system for 2.5x/4x objectives mounted on the condenser carrier.................... 39
2.5.6 0.9/1.25 BF condenser and 0.9 Pol condenser ................................................................. 39
2.5.7 14x40 mm stop sliders for aperture and field diaphragms................................................ 40
2.5.8 Reflector turrets with 4 or 6 coded positions ................................................................... 41
2.5.9 Reflector slider with 2 coded positions ............................................................................ 41
3START-UP........................................................................................................... 42
3.1 Mounting standard components................................................................................ 42
3.1.1 Unpacking and setting up microscope stand ................................................................... 42
3.1.2 Mounting the Axioscope 5 Vario material upper stand part on the stand column ............. 43
3.1.3 Mounting the binocular tube/photo tube ........................................................................ 44
3.1.4 Inserting eyepieces or auxiliary microscope or pinhole diaphragm .................................... 44
3.1.5 Screwing in objectives .................................................................................................... 46
3.1.6 Inserting and removing push-and-click (P&C) reflector modules into the reflector insert.... 47
3.1.7 Mounting the reflector insert.......................................................................................... 48
3.1.8 Mounting the mechanical stage...................................................................................... 48

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3.1.9 Mechanical stages with friction adjustment......................................................................52
3.1.10 Attaching the Pol rotary stage .........................................................................................53
3.1.11 Mounting/removing the 80x60 motorized mechanical stage on the Axioscope 7
motorized material stand ................................................................................................56
3.1.12 Mounting the condenser carrier ......................................................................................57
3.1.13 Mounting the condenser.................................................................................................57
3.1.14 Mounting the darkfield condenser...................................................................................58
3.1.15 Mounting the stage carrier..............................................................................................59
3.1.16 Changing the 12 V, 50 W halogen lamp of the HAL 50 halogen illuminator .....................60
3.1.17 Mounting the illuminator TL LED10 CRI90/RL LED10 CRI90 ..............................................61
3.1.18 Mounting the Axioscope base plate on the stand.............................................................62
3.1.19 Mounting and adjusting the HAL 100 halogen illuminator................................................63
3.1.20 Inserting the adjustment tool of HBO 100 into the stand Bio-TL/RL ...................................66
3.1.21 Mounting and adjusting the HBO 100 illuminator ............................................................66
3.1.22 Mounting the Colibri 3 illumination system and changing the LED modules......................69
3.1.23 Mounting the external illumination fixture HXP 120.........................................................71
3.2 Mounting optional components .................................................................................72
3.2.1 Mounting the intermediate plate for Analyzer Slider ........................................................72
3.2.2 Mounting the tube lens turret .........................................................................................73
3.2.3 Mounting the magnification changer...............................................................................73
3.2.4 Changing the filters in the reflector module FL P&C .........................................................74
3.2.5 Changing the color splitter in the reflector module FL P&C...............................................75
3.2.6 Mounting the polarizer D or the filter holder....................................................................76
3.2.7 Mounting and centering the low-power system for 2.5x/4x objectives ..............................77
3.2.8 Inserting the modulator disk in the 0.9/1.25 BF condenser ...............................................78
3.2.9 Changing the PlasDIC diaphragm ....................................................................................79
3.2.10 Changing the PhC-DIC-PlasDIC diaphragm on the 0.9 BF DF PhC DIC achromatic-
aplanatic condenser ........................................................................................................79
3.2.11 Changing the filter in the filter wheel transmitted light ....................................................80
3.3 Connecting to the power supply and switching the microscope on/off...................81
3.3.1 Microscope stand............................................................................................................81
3.3.2 Connecting the HAL 100 halogen illuminator for transmitted light ...................................82
3.3.3 Connecting the HAL 100 halogen illuminator for reflected light .......................................82
3.3.4 Switching the illumination on/off.....................................................................................83
3.4 Using the Light Manager function..............................................................................84
3.5 Default factory settings of the microscope ................................................................84
4OPERATION ........................................................................................................85
4.1 Default setting of the microscope...............................................................................85
4.1.1 Setting the inter-pupillary distance on the binocular tube .................................................85
4.1.2 Setting the viewing height ..............................................................................................85
4.1.3 Adjusting for ametropia (user's visual impairment) when using eyepiece reticles ...............86
4.2 Illumination and contrast methods in transmitted light microscopy ........................87
4.2.1 Setting up transmitted light brightfield microscopy using the KÖHLER method .................87

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4.2.2 Setting up transmitted light darkfield microscopy using the KÖHLER method ................... 90
4.2.3 Setting up transmitted light phase contrast microscopy ................................................... 93
4.2.4 Setting up transmitted light differential interference contrast (DIC)microscopy ................. 95
4.2.5 Setting up transmitted light PlasDIC contrast microscopy................................................. 97
4.2.6 Setting transmitted light polarization .............................................................................. 98
4.2.7 Setting transmitted light polarization for conoscopic observation - determining the
optical character of crystals........................................................................................... 106
4.3 Illumination and contrast methods in reflected light microscopy .......................... 112
4.3.1 Setting up reflected light brightfield microscopy using the KÖHLER method................... 112
4.3.2 Setting the reflected light darkfield............................................................................... 115
4.3.3 Setting reflected light DIC and reflected light C-DIC ...................................................... 116
4.3.4 Setting reflected light TIC ............................................................................................. 117
4.3.5 Setting reflected light polarization – proof of bireflectance and reflection Pleochroism ... 120
4.3.6 Setting up reflected light fluorescence .......................................................................... 122
5CARE, FUSE REPLACEMENT AND SERVICE ..................................................... 125
5.1 Instrument care ......................................................................................................... 125
5.2 Instrument maintenance........................................................................................... 126
5.2.1 Checking the instrument .............................................................................................. 126
5.2.2 Replacing the fuses in the stand.................................................................................... 126
5.2.3 Replacing the fuses in the 12 VDC 100 W power supply unit......................................... 127
5.3 Troubleshooting........................................................................................................ 128
5.3.1 Microscope .................................................................................................................. 128
5.4 Maintenance and repair work .................................................................................. 131
6ANNEX ............................................................................................................. 132
6.1 List of abbreviations ................................................................................................. 132
6.2 Index .......................................................................................................................... 134
6.3 Industrial property rights.......................................................................................... 138
6.4 System overview....................................................................................................... 138

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LIST OF ILLUSTRATIONS
Fig. 1-1 Warning labels on microscopes with LED light source for transmitted light..........................13
Fig. 1-2 Warning labels on microscopes with HAL 100 for transmitted light and HBO 100 for
reflected light....................................................................................................................14
Fig. 1-3 Warning label on microscopes with LED light source for reflected light ...............................14
Fig. 1-4 Warning label on microscopes with Colibri 3 light source for reflected light ........................15
Fig. 1-5 Warning label on Axioscope 7 stand using the motorized 80x60 mechanical stage 80x60 ...15
Fig. 2-1 Interface diagram (example: Axioscope 5 stand, Bio-TL/RL) .................................................20
Fig. 2-2 Controls and functional elements of the Axioscope 5 stands, Bio-TL ...................................23
Fig. 2-3 Controls and functional elements of the Axioscope 5 stand, Bio-TL/RL ................................25
Fig. 2-4 Controls and functional elements of the Axioscope 5 stand, Pol-TL/RL ................................27
Fig. 2-5 Controls and functional elements of the Axioscope 5 stand, Mat-TL/RL...............................29
Fig. 2-6 Controls and functional elements of the Axioscope 7 stand, Mat-TL/RL mot........................31
Fig. 2-7 Controls and functional elements of the Axioscope 5 Vario material stand..........................33
Fig. 2-8 Binocular photo tube.........................................................................................................36
Fig. 2-9 Binocular ergo photo tube 20°/23 with vertical adjustment ................................................37
Fig. 2-10 Binocular ergo photo tube 15°/23, telescopic with vertical adjustment................................37
Fig. 2-11 Nosepiece with DIN slot for compensators .........................................................................38
Fig. 2-12 Mechanical stage with specimen holder.............................................................................38
Fig. 2-13 Low-power system mounted on the condenser carrier........................................................39
Fig. 2-14 0.9/1.25 BF condenser with modulator disk .......................................................................39
Fig. 2-15 14x40 mm stop slider........................................................................................................40
Fig. 2-16 Reflector turret with 6 coded positions ..............................................................................41
Fig. 2-17 Reflector slider 2 coded positions.......................................................................................41
Fig. 3-1 Setting up the microscope.................................................................................................42
Fig. 3-2 Mounting the Vario upper stand part on the stand column.................................................43
Fig. 3-3 Attaching the binocular tube .............................................................................................44
Fig. 3-4 Inserting eyepieces ............................................................................................................44
Fig. 3-5 Inserting the E-PL 10x/23 GW foc. Pol eyepieces into the binocular photo tube with
upright image ...................................................................................................................45
Fig. 3-6 Inserting the eyepiece reticle..............................................................................................45
Fig. 3-7 Screwing in objectives .......................................................................................................46
Fig. 3-8 Changing the reflector module in the reflector insert..........................................................47
Fig. 3-9 Mounting the reflector insert.............................................................................................48
Fig. 3-10 Changing the mechanical stage, 75x50 R...........................................................................48
Fig. 3-11 Adjusting the X- and Y-axis knob tension...........................................................................49
Fig. 3-12 Changing the rotatable mechanical stage ..........................................................................49
Fig. 3-13 Centering the rotatable mechanical stage ..........................................................................50
Fig. 3-14 Adjusting ergonometric drive.............................................................................................51
Fig. 3-15 Adjusting the frictional momentum ...................................................................................52
Fig. 3-16 Changing the Pol rotary stage with click stop, attachable Pol object guide and stage clips ...53
Fig. 3-17 Centering the Pol rotary stage ...........................................................................................54
Fig. 3-18 Centering objectives..........................................................................................................55
Fig. 3-19 Changing the motorized mechanical stage.........................................................................56

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Fig. 3-20 Plugging in the power cable of the motorized mechanical stage ........................................ 56
Fig. 3-21 Mounting the condenser carrier ........................................................................................ 57
Fig. 3-22 Mounting the condenser .................................................................................................. 57
Fig. 3-23 Mounting the darkfield condenser .................................................................................... 58
Fig. 3-24 Mounting the stage carrier ............................................................................................... 59
Fig. 3-25 Removing the HAL 50 halogen illuminator from the stand ................................................. 60
Fig. 3-26 Inserting halogen lamp 12 V, 50 W................................................................................... 60
Fig. 3-27 Mounting the TL LED10 illuminator or RL LED10 illuminator............................................... 61
Fig. 3-28 Mounting the base plate................................................................................................... 62
Fig. 3-29 Mounting the HAL 100..................................................................................................... 63
Fig. 3-30 Adjusting the HAL 100 ..................................................................................................... 64
Fig. 3-31 Changing the HAL 100..................................................................................................... 65
Fig. 3-32 Inserting the adjustment tool ............................................................................................ 66
Fig. 3-33 Mounting the HBO 100 .................................................................................................... 67
Fig. 3-34 Power supply unit for HBO 100......................................................................................... 67
Fig. 3-35 Adjustment tool ............................................................................................................... 68
Fig. 3-36 Adjusting the HBO 100..................................................................................................... 68
Fig. 3-37 Mounting the illumination system Colibri 3 ....................................................................... 69
Fig. 3-38 Changing the LED modules in the Colibri 3 illumination system.......................................... 70
Fig. 3-39 Mounting the binocular tube with intermediate plate ........................................................ 72
Fig. 3-40 Mounting the magnification changer ................................................................................ 73
Fig. 3-41 Changing the filters in the reflector module FL P&C........................................................... 74
Fig. 3-42 Mounting the filters and the color splitter.......................................................................... 74
Fig. 3-43 Opening the module ........................................................................................................ 75
Fig. 3-44 Changing the color filter................................................................................................... 75
Fig. 3-45 Labeling the color splitter.................................................................................................. 76
Fig. 3-46 Mounting the polarizer D.................................................................................................. 76
Fig. 3-47 Mounting the low-power system ...................................................................................... 77
Fig. 3-48 Inserting the modulator disk in the 0.9/1.25 H condenser .................................................. 78
Fig. 3-49 Mounting the slit-diaphragm ............................................................................................ 79
Fig. 3-50 Changing the PhC-DIC-PlasDIC diaphragm........................................................................ 79
Fig. 3-51 Changing the filter in the filter wheel transmitted light...................................................... 80
Fig. 3-52 Lower stand part for HAL 50 illumination (proceed similarly for LED illumination)................ 81
Fig. 3-53 Lower stand part for HAL 100 illumination ........................................................................ 82
Fig. 3-54 External power supply unit for two lamps (front and back)................................................. 82
Fig. 3-55 Power supply unit for HBO 100 (front and back)................................................................ 83
Fig. 4-1 Setting the inter-pupillary distance on the binocular tube................................................... 85
Fig. 4-2 Setting the viewing height on the binocular tube............................................................... 85
Fig. 4-3 Microscope adjustment in transmitted light brightfield microscopy..................................... 88
Fig. 4-4 Adjusting the height stop on the condenser carrier............................................................ 89
Fig. 4-5 Adjusting the height stop on the focusing drive................................................................. 89
Fig. 4-6 Centering the darkfield diaphragm on the condenser, achromatic-aplanatic 0.9 H D PhC
DIC .................................................................................................................................. 90
Fig. 4-7 Centering the annular phase diaphragm (light-colored, in the condenser) and the phase
ring (dark-colored, in the object) ....................................................................................... 94

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Fig. 4-8 Components for the transmitted light DIC method.............................................................96
Fig. 4-9 Components for transmitted light polarization ...................................................................99
Fig. 4-10 Determination of the polarization direction nγ'in a synthetic fiber ....................................100
Fig. 4-11 Diagram of the Michel-Lévy color tables...........................................................................102
Fig. 4-12 Components for circular polarization contrast ..................................................................105
Fig. 4-13 Determining the optical character....................................................................................110
Fig. 4-14 External power supply unit for HAL 100...........................................................................112
Fig. 4-15 Setting the microscope in the reflected light brightfield ....................................................114
Fig. 4-16 Compensator slot 6x20 mm with C-DIC slider 6x20 mm...................................................116
Fig. 4-17 TIC slider 6x20 mm .........................................................................................................117
Fig. 4-18 Interference stripes..........................................................................................................118
Fig. 4-19 Components for reflected light polarization .....................................................................121
Fig. 4-20 Components for reflected light fluorescence ....................................................................123
Fig. 5-1 Replacing the fuses in the stand ......................................................................................126
Fig. 5-2 Replacing fuses in the power supply unit..........................................................................127

INTRODUCTION
Axioscope 5/7/Vario Notes on instrument safety ZEISS
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1INTRODUCTION
1.1 Notes on instrument safety
The Axioscope 5/7/Vario microscopes were engineered, manufactured and tested in accordance with the
DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 safety standards for electrical measuring, control
and laboratory equipment.
The microscopes fulfill the requirements as stated in Directive 98/79/EC on in vitro diagnostic medical
devices (IVDD) and bear the marking.
The present manual contains information and safety warnings with which the operator must comply.
The following warning, instructional and informational symbols are used in the manual:
CAUTION
Warns the operator of a possible danger.
CAUTION
Warns the operator of hot surfaces.
CAUTION
Warns the operator of the emission of UV radiation.
CAUTION
Warns the operator of the emission of LED radiation.
LED class 3B, max. 30 mW, 365 - 625 nm,
Avoid direct exposure to the beam. Avoid contact with the skin.
CAUTION
Warns the operator of crushing hazard.
CAUTION
Warns the operator to disconnect the instrument from the power supply before opening or
doing any work on the instrument.
ATTENTION
This symbol indicates the risk of damage to the instrument or the system.
NOTE
This symbol indicates information or instructions which must be followed especially carefully.

INTRODUCTION
ZEISS Notes on instrument safety Axioscope 5/7/Vario
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The Axioscope 5/7/Vario microscopes and their original accessories may be used only for the microscopy
procedures described in this operating manual.
The following guidelines are especially important:
CAUTION
The microscope may only be plugged into an electrical outlet equipped with a safety contact.
The safety feature must not be disabled by using an extension cord which does not have a
protective ground conductor.
CAUTION
Whenever it becomes apparent that any of the safety mechanisms are
out of order, the
microscope must be switched off and protected from any inadvertent use.
Please contact the ZEISS Service Department or the Carl Zeiss
Microscopy Service before
switching the microscope on again.
CAUTION
The stands are equipped with integrated power supply units. These units adapt to a voltage
range of 100 to 240 V and a frequency range of 50 to 60 Hz. No further voltage adjustment
is necessary on the microscope itself.
The 12 V DC 100 W external power supply unit for HAL
100 covers a voltage range from
100 to 127 V AC and 200 to 240 V AC ±10%, and a frequency range of 50 to 60
Hz. It
adapts automatically to the applied voltage.
The 24 V DC 30 W external power supply unit for Axioscope 5 Vario covers a voltage range
from 100 V to 240 V and a frequency range of 50 to 60 Hz.
The power supply units for the HBO 100 adapt to a voltage range from 100 to 240 V AC and
a frequency range of 50 to 60 Hz. They adapt automatically to the applied voltage. No further
voltage adjustment is necessary.
Before you switch on the instrument, please verify that the voltage you are about to use is
suitable.
CAUTION
Always disconnect the microscope from the power supply before you open it or change a
fuse!
M
ake sure that the fuses are suitable for the applied nominal current. Never use any
makeshift fuses and do not short-circuit the fuse holders.
CAUTION
The microscopes do not have any special safety devices to protect users from acid or from
potentially infectious, toxic, radioactive or other samples that may be
hazardous to your
health. Compliance with all statutory requirements, especially national accident prevention
regulations, is required when handling such samples.

INTRODUCTION
Axioscope 5/7/Vario Notes on instrument safety ZEISS
03/2018 430035-7344-001 11
CAUTION
Only authorized personnel are permitted to operate the instruments. The personnel must have
been instructed about and be aware of the risks involved in
using the microscope. The
Axioscope is a precision instrument whose functionality
can easily be damaged or even
destroyed when handled improperly.
CAUTION
Operating the instrument in an area with a potentially explosive atmosphere is prohibited.
It may be operated only on stable, non-flammable surfaces.
Specimens must be disposed of appropriately in accordance with applicable statutory
regulations and internal work instructions.
CAUTION
Gas discharge lamps, such as the HBO 100, emit ultraviolet radiation which may cause injuries
to your eyes and skin. Never look directly into the beam and avoid exposing your skin directly
to the beam. Never operate the microscope without the necessary safety equipment (e.g.
special damping f
ilters or the fluorescence protection screen). Gas discharge lamps have a
high internal pressure when hot. Never change the lamps while they are still hot and always
use protective gloves and masks when changing them.
CAUTION
Avoid any contact with the hot lamp case. Before you change a lamp,
disconnect the
instrument from the power supply and wait 15 minutes for the lamp to cool down.
CAUTION
When the lamp module (e.g. Colibri 3) is equipped with appropriate LED modules, or when a
white light source coupled by a fluid light conductor is used, ultraviolet radiation may leak
which can cause injury to your eyes and skin. Never look directly into t
he light and avoid
exposing your skin directly to the light. Always use the appropriate safety equipment when
operating the microscope.
CAUTION
Never look into the light beam of the illumination equipment -
with or without optical
instruments - not
even if you just want to look at a specimen. Irreparable eye damage may
occur!
CAUTION
Do not hold any flammable or easily combustible materials into the light beam.

INTRODUCTION
ZEISS Notes on instrument safety Axioscope 5/7/Vario
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CAUTION
The immersion fluid Immersol 518 Nis irritating to the skin. Avoid any contact with skin,
eyes and clothes. Read the safety data sheets on Immersol 518 N, Immersol 518 Fand
Immersol Wcarefully. If skin contact occurs, wash off with lots of water and soap.
If eye contact occurs, flush with water
immediately for at least 5 minutes. Seek medical
assistance if irritation continues.
CAUTION
Dispose of immersion fluid Immersol 518 N
appropriately: Do not allow to contaminate
surface water or get into drains.
CAUTION
For stands equipped with motorized focusing drives, there is a risk of fingers being crushed in
the working area when the microscope stage is lowered.
Do not reach into the area between the microscope stage/stage carrier/condenser carrier and
the base of the stand while the stage is being lowered.
ATTENTION
The manufacturer assumes no liability for any use of the microscope, its
assemblies or
individual parts if these are used in in a manner or for any purpose other than those described
in this operating manual. This also applies to any maintenance or repair work not performed
by authorized maintenance and repair personnel.
Moreover, the warranty and any claims
thereunder will be voided.
ATTENTION
Microscope lamps radiate a lot of heat which could damage the heat-sensitive fluorescence
filters. Do not remove the heat protection filter when using a fluorescence filter; otherwise the
fluorescence filter may not function properly.
ATTENTION
Dirt and dust may affect the microscope’s performance. Protect it by using a dust cover when
it is not in use. Always make sure that the instrument is switched off before covering it up.
ATTENTION
Closing or covering the ventilation slits may result in heat accumulation which could damage
the instrument and even start a fire. Ensure that the ventilation slits
are always kept open,
uncovered and clear. Do not insert or drop anything into them or let anything fall into them.

INTRODUCTION
Axioscope 5/7/Vario Warning labels on the microscopes ZEISS
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ATTENTION
Always position equipment in a manner that allows easy disconnection from the power
supply.
ATTENTION
Do not use a power supply cord with an inadequate rating.
ATTENTION
When using the HAL or HBO illuminators, a base plate for the Axioscope (000000-2202-526)
is mandatory.
ATTENTION
Do not dispose of defective microscopes in the household waste. Comply with the applicable
statutory regulations for their disposal.
Specimens must also be disposed of appropriately
in accordance with applicable statutory
regulations and internal work instructions.
1.2 Warning labels on the microscopes
Fig. 1-1 Warning labels on microscopes with LED light source for transmitted light

INTRODUCTION
ZEISS Warning labels on the microscopes Axioscope 5/7/Vario
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Fig. 1-2 Warning labels on microscopes with HAL 100 for transmitted light and HBO 100 for reflected
light
Fig. 1-3 Warning label on microscopes with LED light source for reflected light

INTRODUCTION
Axioscope 5/7/Vario Warning labels on the microscopes ZEISS
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Fig. 1-4 Warning label on microscopes with Colibri 3 light source for reflected light
Fig. 1-5 Warning label on Axioscope 7 stand using the motorized 80x60 mechanical stage 80x60

INTRODUCTION
ZEISS Notes on the warranty Axioscope 5/7/Vario
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1.3 Notes on the warranty
The manufacturer guarantees that the instrument is free of any material and workmanship defects upon
delivery. If you become aware of any deficiencies, please contact us immediately and take all necessary
precautions in order to avoid further damage. Upon notice of deficiencies it is up to the manufacturer’s
discretion to correct the deficiencies or to deliver a defect-free instrument. Defects due to ordinary wear
and tear (especially on wearing parts) and to improper handling are not covered by our warranty.
The manufacturer is not liable for damage to the instrument due to incorrect operation, negligence or
any other manipulation of the instrument, in particular due to the removal or replacement of instrument
parts or due to the use of accessories from other manufacturers. This will immediately void the warranty.
No maintenance or repair work, except for the instances mentioned in the manual, may be performed on
the microscopes. Only ZEISS service personnel or personnel authorized especially by ZEISS may perform
repair work on the microscopes. In the event of malfunction of your instrument, please contact the ZEISS
Microscopy Service Department (see page 131) or the ZEISS agency assigned to your country.

DESCRIPTION OF THE INSTRUMENT
Axioscope 5/7/Vario Intended use ZEISS
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2DESCRIPTION OF THE INSTRUMENT
2.1 Intended use
The Axioscope 5/7/Vario microscopes are universal microscopes designed especially for applications in
biological and medical fields and for materials analysis.
Depending on the configuration of the microscope stand, they may be used with transmitted light only or
with a combination of transmitted light and reflected light.
Typical areas of use for the Axioscope 5/7/Vario in biomedical applications include:
−medical analysis in laboratories, hospitals, physicians’ practices,
−science and research (colleges, universities) in medical and biological fields,
−industrial applications (pharmacology, food technology),
−analysis of blood and tissue samples of the human body.
The Axioscope 5/7/Vario may also be used for materials analysis in:
−routine research in aerospace and metallographic laboratories,
−quality assurance and control in the automobile and mechanical engineering industries
−failure analysis in the electronics and semiconductor industries,
−The analysis of optical properties of minerals in geoscience studies, and
−morphology studies in the chemical, polymer and mineral exploration industries.
Depending on the accessories with which each instrument is equipped, the following microscopy and
contrasting techniques can be performed:
Transmitted light
Reflected light
−
Bright-field (BF)
−
Bright-field (BF)
−
Dark-field (DF)
−
Dark-field (DF)
−
Phase contrast (PhC)
−
Differential interference contrast (DIC)
−
Differential interference contrast (DIC)
−
Circular differential interference
contrast/total interference contrast
(C-DIC/TIC)
−
PlasDIC
−
Fluorescence
−
Polarization (Pol): orthoscopy and
conoscopy
−
Polarization (Pol)
The binocular photo tubes can be connected via a special adapter with a microscopy camera, a reflex
camera or a digital/video camera for documentation purposes. An Infinity Space Camera Port (ISCP)
camera can be used when working with transmitted light.

DESCRIPTION OF THE INSTRUMENT
ZEISS Technical data Axioscope 5/7/Vario
18 430035-7344-001 03/2018
2.2 Technical data
Dimensions (length x width x height)
Microscope stand for Axioscope 5/7......................................... approx. 293.5 mm x 240 mm x 367.5 mm
Microscope stand for Axioscope Vario......................................... approx. 429 mm x 458.5 mm x 700 mm
Weight
Microscope stand for Axioscope 5/7 ........................................................................................................
(depending on stand configurations and accessories)..................................................approx. 14 to 20 kg
Microscope stand for Axioscope Vario.................................................................................approx. 32 kg
Ambient conditions
Shipping (in packaging):
Permissible ambient temperature....................................................................................-40 to +70 °C
Permissible humidity (no condensation) ................................................................. max. 75% at 35 °C
Storage:
Permissible ambient temperature...................................................................................+10 to +40 °C
Permissible humidity (no condensation) ................................................................. max. 75% at 35 °C
Operation:
Permissible ambient temperature...................................................................................+10 to +40 °C
Permissible relative humidity (no condensation)...................................................... max. 75% at 35 °C
Highest permitted altitude of use ....................................................................................max. 2000 m
Air pressure ........................................................................................................800 hPa to 1060 hPa
Degree of pollution...........................................................................................................................2
Operational specifications
Operational area ................................................................................................................Closed rooms
Protective class.......................................................................................................................................I
Protection type................................................................................................................................ IP 20
Electrical safety............................................................in accordance with DIN EN 61010-1 (IEC 61010-1)
............................................................................................in conjunction with CSA and UL regulations
Overvoltage category.............................................................................................................................II
RFI suppression.......................................................................................conforming to EN 55011 Class B
Noise immunity ..................................................................................... conforming to DIN EN 61326/A1
Mains voltage for the Axioscope 5/7
with internal power supply unit.......................................................................................100 to 240 V
Mains voltage for the Axioscope Vario
with external power supply unit ......................................................................................100 to 240 V
Mains frequency....................................................................................................................50 to 60 Hz
Power consumption of the Axioscope 5
with internal power supply unit................................................................................................. 60 VA
Power consumption of the Axioscope 7
with internal power supply unit............................................................................................... 100 VA
Power consumption of the Axioscope Vario
with external power supply unit ................................................................................................ 30 VA

DESCRIPTION OF THE INSTRUMENT
Axioscope 5/7/Vario Technical data ZEISS
03/2018 430035-7344-001 19
HBO 100 W power supply unit
Range of application..................................................................................................................... indoor
Protection class.......................................................................................................................................I
Protection type ................................................................................................................................IP 20
Mains voltage ........................................................................................................100 VAC ... 240 VAC
...............................................................................................................No voltage adjustment required
Mains frequency................................................................................................................... 50 to 60 Hz
Power consumption with HBO 103 in operation ...........................................................................155 VA
Fuses in accordance with IEC 127
Axioscope 5/7 microscope stand for LED illumination in transmitted light: .............2 T 3.15 A/H, 5x20 mm
Axioscope 5/7 microscope stand for HAL 50 illumination in transmitted light:....... 2 T 3.15 A/H, 5x20 mm
HBO 100 W power supply unit .................................................................................T 2.0 A/H, 5x20 mm
12 V DC 100 W external power supply unit............................................................2 T 5.0 A/H, 5x20 mm
Light sources
LED illumination transmitted light/reflected light
Power consumption ...........................................................................................................max. 10 W
Adjustment of light source .............................................................. continuous approx. 10 to 800 mA
Halogen lamp.........................................................................................................................12 V/50 W
Adjustment of light source .................................................... infinitely variable from approx. 3 to 12 V
Halogen lamp.......................................................................................................................12 V/100 W
Adjustment of light source .................................................... infinitely variable from approx. 3 to 12 V
Mercury vapor short arc lamp............................................................................................. HBO 103 W/2
Power consumption for HBO 103 W/2 ......................................................................................100 W
Colibri 3 illumination system
Power consumption ...................................................................................................................15 W
Axioscope 5/7/Vario
Stand with manual/motorized stage focusing
Coarse drive .................................................................................................... approx. 4 mm/rotation
Fine drive...........................................................approx. 0.4 mm/rotation; approx. 4 µm scale intervals
Lifting range ............................................................................................................... approx. 25 mm
Height stop.........................................................................................................mechanically variable
Condenser 0.9/1.25 H with optional
modulator disk...................................................................................... for bright-field, dark-field and
........................................................................................................ phase contrast 1, 2, 3 or PlasDIC
Manual objective change ........................................................................via nosepiece, 6-positions, M27
Manual reflector module change .................................................... via reflector slider, 2-positions coded,
.............................................................................reflector turret 4-positions coded or 6-positions coded

DESCRIPTION OF THE INSTRUMENT
ZEISS Interface diagram Axioscope 5/7/Vario
20 430035-7344-001 03/2018
2.3 Interface diagram
The following figure shows a diagram of the microscope’s hardware interfaces. The Axioscope 5 stand
Bio-TL/RL with LED/HAL illumination serves as the example.
Fig. 2-1 Interface diagram (example: Axioscope 5 stand, Bio-TL/RL)