3D Histech PANNORAMIC CONFOCAL 3.0.3 User manual

PANNORAMIC® CONFOCAL
3.0.3
USER’S GUIDE
March 30, 2021 –Rev.1

PANNORAMIC® CONFOCAL 3.0.3
TABLE OF CONTENTS
MARCH 30, 2021
1
Table of Contents
Table of Contents.................................................................................................................................... 1
Disclaimer ............................................................................................................................................... 4
Declaration of Conformity..................................................................................................................... 6
About this User's Guide ..........................................................................................................................7
Character Formats and Symbols ........................................................................................................... 8
Notes Regarding Operational Safety .................................................................................................... 9
Notes on Warranty................................................................................................................................ 13
Terms and Abbreviations......................................................................................................................14
1Product Description ...................................................................................................................... 15
1.1 Product overview 15
1.1.1 Intended use...................................................................................................................... 15
1.1.2 Features and benefits .......................................................................................................16
1.1.3 Warning and information labels....................................................................................... 17
1.1.4 Base unit ............................................................................................................................ 17
1.1.5 Connector panel................................................................................................................18
1.2 System overview 18
1.2.1 Pannoramic® Confocal System ........................................................................................19
1.2.2 Hardware options ............................................................................................................ 20
1.2.3 Control software ............................................................................................................... 21
2Installation.....................................................................................................................................22
2.1 Operation system of the control computer 23
2.2 Control computer ports 24
2.3 Connectors and cables 25
2.4 Power the scanner on/off 25
3Operating Slides and Trays .......................................................................................................... 26
3.1 Preparing slides and trays 26
3.2 Affixing barcode stickers to slides 28
3.3 Inserting and removing slides 29
4Software Description ....................................................................................................................32
4.1 Starting / exiting Pannoramic® Scanner software 32
4.1.1 Starting the software........................................................................................................32
4.1.2 Exiting the software..........................................................................................................33
4.2 Main window functions 33

PANNORAMIC® CONFOCAL 3.0.3
TABLE OF CONTENTS
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5Scanning Modes and Settings ..................................................................................................... 54
5.1 Brightfield scanning mode 54
5.1.1 Profile settings panel ........................................................................................................55
5.1.2 Microscope control panel................................................................................................ 62
5.1.3 Quick preview functions ...................................................................................................73
5.1.4 Quick microscope functions .............................................................................................73
5.2 Fluorescence microscope mode 75
5.2.1 Profile settings panel ........................................................................................................75
5.2.2 Microscope control panel................................................................................................ 86
5.2.3 Quick preview functions .................................................................................................. 99
5.2.4 Quick microscope functions ............................................................................................ 99
5.2.5 Bottom menu bar of the main window .........................................................................102
5.3 Planner mode 103
5.3.1 General functions at the main menu..............................................................................104
5.3.2 General functions at the tray menu ...............................................................................106
5.3.3 General functions at the slide menu ..............................................................................107
5.3.4 Slide view with card ....................................................................................................108
5.3.5 Table view mode ..............................................................................................................112
5.3.6 Card collection view mode ..........................................................................................113
5.4 Profile and preview overview on the planner 113
5.5 Progress features 114
6Settings and service .................................................................................................................... 116
6.1 Settings 116
6.2 Service 126
7Hosting......................................................................................................................................... 135
8Using Profiles............................................................................................................................... 137
9Troubleshooting and Maintenance............................................................................................139
9.1 Recovery functions 139
9.2 Safety information 139
9.2.1 Cleaning the device .........................................................................................................140
9.2.2 Cleaning the objectives...................................................................................................140
9.2.3 Cleaning the trays........................................................................................................140
9.2.4 Preventive maintenance..............................................................................................141
9.3 Troubleshooting 141

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9.3.1 Fluorescent applications.................................................................................................143
9.4 Maintenance 144
9.4.1 Opening the service door ...............................................................................................144
9.4.2 Replacing fuses of the main power switch................................................................145
9.4.3 Removing and refilling the pipette ............................................................................146
9.4.4 Removing a slide .........................................................................................................149
9.4.5 Installing and removing filter cubes...........................................................................150
9.4.6 Transporting ................................................................................................................ 153
9.4.7 Removing and cleaning the condenser......................................................................154
10 Technical Data ............................................................................................................................. 157

PANNORAMIC® CONFOCAL 3.0.3
DISCLAIMER
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Disclaimer
Copyright © 2001-2021 3DHISTECH Ltd.
All rights reserved.
Intellectual property related to the 3DHISTECH products are as follows:
•P1300124; PCT/HU2014/000020; EP 14745228.8.; US 14/771.380
•P0700404; PCT/HU2008/000065; EP 08762668.5.; US 12/663.537
•P1700279; PCT/HU2018/050030
•P1700483
•P1500394; PCT/HU2016/050039; EP 16840280.8.; US 15/756.370
•P0700409; US 2008/0306964
•P0800181; PCT/HU2009/000027; EP 09721248.4.
•P0600435; PCT/HU2007/000047; EP 2024772; US 12/301.781
•P0600177; PCT/HU2007/000018; EP 1.994.398; US 12/281.566
•P0900741; PCT/HU2010/000130; EP 10812989.1.
•P0900142; PCT/HU2010/000027; EP 10726183.6
•P101036; PCT/IB2005/050351; EP 1789831; US 7.663.078
•P0301911; PCT/HU04/00019; EP 1644767; US 7327515
The above mentioned granted patents, granted utility model, registered trademarks, pending
international and national patent applications and the other corresponding IP are the sole
properties of the 3DHISTECH Ltd.
3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to,
general or specific damage, indirect damage, consequential damage or incidental damage,
including the results of the analysis of the digitized slides, for example, change of health status
related to erroneous diagnosis from the digitized slide(s)) that stems from or is associated with
use of Product, digitized slides, quality of staining, quality of stained slides, quality of used
method of staining. 3DHISTECH Ltd. assumes no responsibility for the functionality and fault-
free condition of your “application programs” (Workflows, VBA macros, Commander scripts).

PANNORAMIC® CONFOCAL 3.0.3
DISCLAIMER
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CAUTION
For research and education uses only, not for use in diagnostic procedures. This product has not
been approved or cleared as a medical device by the U.S. Food and Drug Administration, nor any
other regulatory authority. The data and images obtained or viewed using this product are not
intended for clinical or diagnostic use.
For performance evaluation only, the performance characteristics of this product have not been
established.
Third-party disclaimer
3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general
or specific damage, indirect damage, consequential damage or incidental damage, including
damage resulting from lost profits, interruption of business, loss of business information,
pecuniary loss or similar damage) that stems from or is associated with the incorporated part of
this Product that is made by a third-party company.
THE SOFTWARE IS PROVIDED “AS IS” AND WITHOUT WARRANTY OF ANY KIND, EXPRESSED,
IMPLIED OR OTHERWISE, INCLUDING WITHOUT LIMITATION, ANY WARRANTY OF
MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
Further Information
For the latest information on 3DHISTECH products and services, please visit our website at the
following URL: http://www.3dhistech.com.
Company Address
3 Öv Street, 1141 Budapest –HUNGARY

PANNORAMIC® CONFOCAL 3.0.3
DECLARATION OF CONFORMITY
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Declaration of Conformity
3DHISTECH Ltd. declares that the product Pannoramic® Confocal is designed and produced with
consideration of the quality management system certified according to the ISO 13485:2003
standard, and conforms to the directives and other standards listed below:
IEC 61010-1:2010 (ed. 3), “Safety requirements for electrical equipment for measurement, control,
and laboratory use –Part 1: General requirements”
EN 61326-1:2013 “Electrical equipment for measurement, control and laboratory use – EMC
requirements –Part 1: General requirements”
EN 61010-1:2010 Safety requirements for electrical equipment for measurement, control, and
laboratory use –Part 1: General requirements
EN 55011:2009+A1:2010 Industrial, scientific and medical equipment. Radio-frequency disturbance
characteristics. Limits and methods of measurement
EN 61000-3-2:2006+A1:2009+A2:2009 Electromagnetic compatibility (EMC). Limits. Limits for
harmonic current emissions (equipment input current ≤ 16 A per phase)
EN 61000-3-3:2013 Electromagnetic compatibility (EMC) –Part 3-3: Limits –Limitation of voltage
changes, voltage fluctuations and flicker in public low-voltage supply systems, for equipment with
rated current ≤16 A per phase and not subject to conditional connection
During the design, development, and production process of this device, the product safety
standard ISO 14971:2007, Medical Devices—The application of risk management to medical devices
was applied. The standard provides a process framework and associated requirements for
management responsibilities, risk analysis and evaluation, risk controls and lifecycle risk
management.
Conformity with Directive 2014/30/EU is marked by on the Pannoramic® Confocal device.
Further information may be obtained from the manufacturer:
3DHISTECH Ltd.
3 Öv Street, 1141 Budapest –HUNGARY

PANNORAMIC® CONFOCAL 3.0.3
ABOUT THIS USER'S GUIDE
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About this User's Guide
The purpose of this manual is to assist the user in the safe and reliable operation of the product
described. Before operating the device, you must carefully read this instruction manual, and
adhere to all WARNING and CAUTION notices (refer to chapter Notes Regarding Operational
Safety for more information), and a copy should be kept in the close proximity or near the
scanner for reference whenever required.
This User's Guide is a part of the Pannoramic® Confocal system identified by the following article
number:
FH-CONF-000-00-2

PANNORAMIC® CONFOCAL 3.0.3
CHARACTER FORMATS AND SYMBOLS
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Character Formats and Symbols
Example (text in bold)
Words or textual elements that appear on the user interface (UI), such as field names, screen and
window titles, buttons and menu names, function or option texts.
Keys or hotkeys of the keyboard. For example, function keys (e.g. F11, or Enter) or the Ctrl+M key
combination.
Example (text in bold and italics)
Cross-references within this document or to other documents.
Warning!
Can cause irreversible damage or harm to the user of the product or the product itself if this
instruction is not followed.
Caution!
Can cause the product to not work properly if this instruction is not followed and alerts to a
potentially critical situation.
Notice:
Calls attention to some important information or feature.
Special attention should be paid to all information provided and the procedures described in
chapter Safety information.

PANNORAMIC® CONFOCAL 3.0.3
NOTES REGARDING OPERATIONAL SAFETY
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Notes Regarding Operational Safety
The below section of this User’s Guide contains information and warnings of a kind that must be
followed by owner/operator personnel.
Warning and advisory symbols which are used on the base unit of the Pannoramic® Confocal and
in chapter Warning and information labels have the following meanings:
Caution!
Disconnect mains plug before any kind of intervention into the device!
Caution!
Crush hazard!
The Pannoramic® Confocal, including any of its original accessory components, should not be
operated in any other way than described in chapter Intended use.
Any of the following notes should be strictly observed:
Caution!
In the event of any non-conforming type of use, including non-conforming use of assemblies
or single parts, no liability will be assumed by the Manufacturer. This shall also apply to
service or repair work of any kind, which is performed by other than authorized service
personnel. All claims for warranty and warranty services will be null and void in any such
case.

PANNORAMIC® CONFOCAL 3.0.3
NOTES REGARDING OPERATIONAL SAFETY
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Warning!
Insert the power cord plugs only into a grounding outlet. The protective action must not be
rendered ineffective by extension cables without protective conductor. The Pannoramic®
Confocal has a built-in protection which must not be rendered ineffective by extension
cables without protective (earth-ground) conductor.
Warning!
The operation of the instrument in explosion-risk environments is not allowed.
Warning!
If protective devices are found to be ineffective, the Pannoramic® Confocal must be shut
down and precautions must be taken to prevent unintended usage. For necessary repair
work or work to restore proper operating condition, you should contact the 3DHISTECH
Customer Service.
Notice:
Electric power to the Pannoramic® Confocal is supplied via an internal mains power supply
unit. It allows for operation at line voltages in the range of 100 V to 240 V ±10%, 50-60 Hz,
without any extra facilities required for voltage transformation.
Warning!
Check that your local line voltage is compatible with Pannoramic® Confocal specifications
before you turn power on.

PANNORAMIC® CONFOCAL 3.0.3
NOTES REGARDING OPERATIONAL SAFETY
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Disconnect plug!
Remember to unplug the power cord before you open the Pannoramic® Confocal base unit
or replace a fuse!
Warning!
Only connect UL-approved SELV (safety extra low voltage) rated external devices to the
instrument to avoid the risk of electrical shock.
Warning!
The Pannoramic® Confocal does not include any special facilities to protect from samples
with an etching, potentially infectious, toxic, radioactive, or other health-damaging effect.
You are under obligation to comply with all currently binding legislation, notably, national
accident prevention rules when handling samples of this kind.
Caution!
Dirt or dust may adversely impact the operating capability of the Pannoramic® Confocal. For
this reason, measures must be taken to eliminate such influences to a maximum possible
degree. During periods of non-use, the Pannoramic® Confocal must be protected with a
dust cover. Check that power is turned off before you put the cover on.

PANNORAMIC® CONFOCAL 3.0.3
NOTES REGARDING OPERATIONAL SAFETY
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Crush hazard!
There is crush hazard at the following areas of the device:
The tray feeder door, the cross-belt area of the tray guide, and the safety plate during
operation. Please note that the digitizing process has to be interrupted and the tray moved
via software to the output position before removal.
The two hinges of the service door.
The inner side of the door opener mechanism.
When reaching by the pipette unit into the farthest area of the device.
Warning!
A defective Pannoramic® Confocal is not classified as domestic waste. It must be properly
disposed in accordance with currently valid legal requirements.
Warning!
The Pannoramic® Confocal may not be operated by other than properly instructed persons.
Operating personnel must be fully aware of the potential dangers which a particular field of
application involves. The Pannoramic® Confocal is a precision instrument which may suffer a
significant reduction in operational functions or even physical destruction following
intervention performed in any nonconforming manner.
Warning!
If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.

PANNORAMIC® CONFOCAL 3.0.3
NOTES ON WARRANTY
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Notes on Warranty
3DHISTECH Ltd. –as the Product Manufacturer –warrants the Pannoramic® Confocal to be free
from faults in material and workmanship at the moment of installation. Defects must be notified
immediately on identification and maximum efforts must be undertaken in order to minimize
damage. On receiving notice of a fault, the Product Manufacturer will be under obligation to
remove the fault, at his own option, either by providing adequate repair services or by delivering
a faultless product for replacement. No warranty will be accepted in cases of natural wear
(notably, wear and tear) or improper product handling.
The Product Manufacturer will refuse any liability in the event of damage resulting from faulty
operation, negligence or unauthorized intervention into the product, notably, from the
replacement of product parts or the usage of accessory parts from other manufacturers. Any
such case will void the user’s claim for warranty.
Except for actions which are explicitly described in this User’s Guide, you are prohibited from
performing any kind of maintenance or repair work at the Pannoramic® Confocal. Only
authorized 3DHISTECH Service personnel or specifically authorized representatives are allowed to
handle repair work. In case of malfunction contact 3DHISTECH Service Support first.

PANNORAMIC® CONFOCAL 3.0.3
TERMS AND ABBREVIATIONS
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Terms and Abbreviations
Term/Abbreviation
Description
CSV
Comma Separated Value
FOV
Field of View
NA
Numerical Aperture
Virtual Slide / Slide
A digital image of a thin glass plate on which specimens are mounted for microscopic
study:
a dynamic, interactive image that you can manage, save, magnify, zoom, name, evaluate,
annotate, mark, comment, send to a colleague electronically for co-operation or advice,
and so on.
Table 1: Terms and abbreviations

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PRODUCT DESCRIPTION
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1Product Description
1.1 Product overview
1.1.1 Intended use
The Pannoramic® Confocal is a digital scanner microscope installed with an Aurox CC88 confocal
unit and high-numerical aperture immersion and/or dry objectives designed to use transmitted
light and reflected light fluorescence to digitize histopathology slides (histological sections,
typically) based on confocal principles. The target users of the product can work for example, in
education, research, industry or medical sectors.
Warning!
The Pannoramic® Confocal device must be operated by a properly instructed person only.
Operating personnel must be fully aware of the potential dangers which a particular field of
application involves. The Pannoramic® Confocal is a precision instrument which may suffer a
significant reduction in operational functions or even physical destruction following
intervention performed in any nonconforming manner.
Warning!
After each scanning make sure to inspect the digital slide for over-exposed areas. In case the
slide is scanned based on improperly set parameters, it can highly affect the diagnostic
result because of information loss.
Warning!
Any modification to a hardware or software component of the PC will highly affect the
functionality of the scanner program.
Warning!
Do NOT install any other application than the Scanner control software application and its
auxiliary drivers on the control computer.

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PRODUCT DESCRIPTION
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1.1.2 Features and benefits
The Pannoramic® Confocal offers world-class brightfield and fluorescent whole slide scanning. An
outstanding 0.16 µm/pixel (both in Brightfield and Fluorescent modes) resolution is achieved with
the 40×/1.2 NA (equivalent to 62× magnification) C-Apochromat objective.
Key features of the Pannoramic® Confocal scanner:
•Outstanding image resolution achieved by using Aurox CC88 confocal unit
•Widefield Fluorescent, Confocal Fluorescent, and Brightfield imaging
•Darkfield preview for low intensity samples
•Pre-Scan function for FL preview imaging
•Up to 4 filter cubes (quad or dual) for fluorescence
Notice:
The number of channels available depends on the type of the applied fluorescent
illumination device.
•High-end fluorescent illumination device –Lumencor SPECTRA 7 Light Engine
•Multiband filter support
•FL scanning with a low-noise, 16-bit scientific CMOS pco.edge 5.5 camera
•Software-aimed flat-field correction compensation method to create
homogeneous slide image
•12-slide automatic loading and scanning
•Motorized objective changer
•Automated immersion for high NA objectives
•Improved permanent autofocus
•Compact and robust device
•Good quality and stable manufacturing
•High-resolution preview camera for 1D and 2D barcode reading
•Image sharpening (for both Fluorescence and Brightfield) feature to improve the
visual appearance of digital slides
•Focus algorithm finds the focus which is most appealing to the human eye
Pannoramic® Confocal is an optimal choice for customers who want to scan in brightfield and in
fluorescent mode where the quantity of scanned slides will be regularly over 10 slides per day.

PANNORAMIC® CONFOCAL 3.0.3
PRODUCT DESCRIPTION
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1.1.3 Warning and information labels
The following figure contains the labels used on the back panel, and on the inside of Pannoramic®
Confocal main unit.
Figure 1: Warning and information labels
1.1.4 Base unit
The following figure shows the main outer parts of the device.
Figure 2: Main parts of the base unit

PANNORAMIC® CONFOCAL 3.0.3
PRODUCT DESCRIPTION
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1) Service door
2) Door securing lock
3) Slide tray feeder door
4) Cable outlet
5) Connector panel (USB port, power supplyconnector, fuse box)
6) Transport handle (4x at the four corners of the device)
7) On/Off button
1.1.5 Connector panel
The following figure details the sub-assemblies of the connector panel.
Figure 3: Sub-assemblies of the connector panel
1) USB port for controller PC
2) Fuse box
3) Main power switch
4) Main power inlet
1.2 System overview
The standard scope of delivery of the product includes the following hardware and software
components.

PANNORAMIC® CONFOCAL 3.0.3
PRODUCT DESCRIPTION
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1.2.1 Pannoramic® Confocal System
The following figure shows the main parts of the Pannoramic® Confocal system.
Figure 4: Pannoramic® Confocal system overview
1) Pannoramic® Confocal base unit
2) Slide tray
3) Control computer
4) Monitor
5) Keyboard
6) Mouse