3D Histech Pannoramic SCAN 150 User manual

PS1.17-UG-Rev1
Pannoramic SCAN 150
1.17
User’s Guide
July 4, 2013
Rev. 1

Pannoramic SCAN 150 1.17 User’s Guide
Contents
Disclaimer....................................................................................................................... 5
Character Formats and Sym ols....................................................................................... 7
Declaration of Conformity............................................................................................... 8
Notes Regarding Operational Safety................................................................................9
Notes on Warranty........................................................................................................ 11
Terms and A reviations............................................................................................... 12
1 Product Description.................................................................................................... 13
1.1 Product Overview....................................................................................................................13
1.1.1 Intended U e..................................................................................................................................................13
1.1.2 Feature and Benefit .....................................................................................................................................13
1.1.3 Warning and Information Label ....................................................................................................................14
1.1.4 Ba e Unit........................................................................................................................................................15
1.2 Sy tem Overview......................................................................................................................16
1.2.1 Pannoramic SCAN 150 y tem........................................................................................................................16
1.2.2 Hardware Option ..........................................................................................................................................17
1.2.3 Control Software............................................................................................................................................20
2 Installation................................................................................................................. 21
2.1 Control computer port ...........................................................................................................21
2.2 Connector and cable .............................................................................................................22
3 Operating Slides and Magazines..................................................................................24
3.1 Preparing Slide and Magazine ..............................................................................................24
3.2 Affixing Barcode Sticker to Slide ...........................................................................................25
3.3 In erting and Removing Slide .................................................................................................26
4 Software Description.................................................................................................. 30
4.1 Starting / Exiting Pannoramic Scanner Software.....................................................................30
4.1.1 Starting the oftware......................................................................................................................................30
4.1.2 Exiting the oftware........................................................................................................................................31
4.2 Option .....................................................................................................................................31
4.2.1 Barcode Setting .............................................................................................................................................31
4.2.2 Image Compen ation.....................................................................................................................................34
4.2.3 Micro cope Setting .......................................................................................................................................37
4.2.4 External Application Call Setting ...................................................................................................................41
4.3 U ing Brightfield Manual Mode...............................................................................................42
4.3.1 Routine work tab............................................................................................................................................44
4.3.2 Scan Option window.....................................................................................................................................46
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Pannoramic SCAN 150 1.17 User’s Guide
4.3.3 Preview tab.....................................................................................................................................................48
4.3.4 Preview area canned by preview camera.....................................................................................................51
4.3.5 Focu tab........................................................................................................................................................57
4.3.6 Service tab......................................................................................................................................................59
4.3.7 Di play field and toolbar for live image ........................................................................................................60
4.3.8 Start Scan........................................................................................................................................................62
4.4 U ing Brightfield Automatic Mode..........................................................................................62
4.4.1 Routine work tab............................................................................................................................................62
4.4.2 Barcode tab...................................................................................................................................................64
4.4.3 Preview tab.....................................................................................................................................................68
4.4.4 Focu tab........................................................................................................................................................69
4.4.5 Service tab......................................................................................................................................................70
4.4.6 Start Scan........................................................................................................................................................71
4.5 U ing Fluore cent Manual Mode.............................................................................................72
4.5.1 Routine work tab............................................................................................................................................72
4.5.2 Scan Option ...................................................................................................................................................77
4.5.3 Preview tab.....................................................................................................................................................81
4.5.4 Hardware tab..................................................................................................................................................84
4.5.5 Service tab......................................................................................................................................................87
4.6 U ing Fluore cent Automatic Mode........................................................................................88
4.6.1 Routine work tab............................................................................................................................................88
4.6.2 Barcode tab...................................................................................................................................................93
4.6.3 Preview tab panel...........................................................................................................................................96
4.6.4 Focu tab panel...............................................................................................................................................97
4.6.5 Service tab panel............................................................................................................................................97
4.7 Scan To Server..........................................................................................................................98
4.8 U ing Profile .........................................................................................................................105
5 Trou leshooting and Maintenance............................................................................107
5.1 Safety Information.................................................................................................................107
5.1.1 Cleaning the device......................................................................................................................................108
5.1.2 Cleaning the magazine.................................................................................................................................108
5.1.3 Preventive maintenance...............................................................................................................................108
5.2 Trouble hooting.....................................................................................................................109
5.2.1 Fluore cent Application (optional).............................................................................................................112
5.3 Maintenance..........................................................................................................................113
5.3.1 Removing the protective cover ...................................................................................................................113
5.3.2 Replacing the halogen lamp.........................................................................................................................114
5.3.3 Replacing Lamp Module (for Fluore cent Option).......................................................................................116
5.3.4 Removing a lide...........................................................................................................................................118
5.3.5 In talling and Removing the Filter Module (Fluore cent Option)................................................................120
5.3.6 Removing the magazine from the tran porting y tem...............................................................................121
5.3.7 Tran porting Pannoramic SCAN 150.............................................................................................................123
6 Technical Data........................................................................................................... 125
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Pannoramic SCAN 150 1.17 User’s Guide
Index........................................................................................................................... 130
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Pannoramic SCAN 150 1.17 User’s Guide
Disclaimer
Copyright © 2001-2013 3DHISTECH Ltd.
All right re erved.
Intellectual property related to the 3DHISTECH product are a follow :
EP 1,789,831; US 7,663,078; EP 2,024,772; JP 2009-512685; US 12/301,781; EP 1,644,767; JP 2007-
516451; US7,327,515; EP 1,994,398; JP 2008-557835; US 12/281,566; U0700210; US 6,246,785;
HU0900142; HU0700404; PCT/HU2007/000065; EP 08762668.5; US 12/663,537;
PCT/AT2006/000492; JP 2008-542552; EP 06817469; US 12/095,545; PCT/AT2006/000493; EP
06817470.5; US 12/095,596; HU0900741; US 61/264,732; US 11/826,752; P0401870;
PCT/IB2005/050344; US 11/662,976; HU0700409; US 12/042,050; US 77/636,144 and 007502032.
The above mentioned granted patent , granted utility model, regi tered trademark , pending
international and national patent application and the other corre ponding IP are the ole propertie
of the 3DHISTECH Ltd.
3DHISTECH Ltd. is not lia le for damage of whatever nature (including, ut not limited to, general
or specific damage, indirect damage, consequential damage or incidental damage, including the
results of the analysis of the digitized slides, for example, change of health status related to
erroneous diagnosis from the digitalized slide(s)) that stems from or is associated with use of
Product, digitalized slides, quality of staining, quality of stained slides, quality of used method of
staining. 3DHISTECH Ltd. assumes no responsi ility for the functionality and fault-free condition of
your “application programs” (Workflows, VBA macros, Commander scripts).
CAUTION
Thi product ha not been approved or cleared a a medical device by the U.S. Food and Drug
Admini tration.
Do not u e thi device in clo e proximity to ource of trong electromagnetic radiation (e.g.
un hielded intentional RF ource ), a the e may interfere with the proper operation.
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Pannoramic SCAN 150 1.17 User’s Guide
Third-Party Disclaimer (Li TIFF – TIFF Li rary and Utilities)
3DHISTECH Ltd. i not liable for damage of whatever nature (including, but not limited to, general or
pecific damage, indirect damage, con equential damage or incidental damage, including damage
re ulting from lo t profit , interruption of bu ine , lo of bu ine information, pecuniary lo or
imilar damage) that tem from or i a ociated with the incorporated part of thi Product that i
made by Silicon Graphic Inc.
THE SOFTWARE IS PROVIDED “AS IS” AND WITHOUT WARRANTY OF ANY KIND, EXPRESSED,
IMPLIED OR OTHERWISE, INCLUDING WITHOUT LIMITATION, ANY WARRANTY OF
MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
IN NO EVENT SHALL SAM LEFFLER OR SILICON GRAPHICS BE LIABLE FOR ANY SPECIAL, INCIDENTAL,
INDIRECT OR CONSEQUENTIAL DAMAGES OF ANY KIND, OR FOR ANY DAMAGES WHATSOEVER
RESULTING FROM LOSS OF USE, DATA OR PROFITS, WHETHER OR NOT ADVISED OF THE POSSIBILITY
OF DAMAGE, AND ON ANY THEORY OF LIABILITY, ARISING OUT OF OR IN CONNECTION WITH THE
USE OR PERFORMANCE OF THIS SOFTWARE.
Further Information
For the late t information on 3DHISTECH product and ervice , plea e vi it our web ite at the
following URL: http ://www.3dhi tech.com .
Company Addre : 29-33 Konkoly-Thege Miklo treet, Building 6
1121 Budape t – HUNGARY
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Pannoramic SCAN 150 1.17 User’s Guide Character Formats and Symbols
Character Formats and Sym ols
Example Word or character that appear on the creen. The e include field name , creen
and window title , pu hbutton and menu name , path or option .
Key on the keyboard. For example, function key ( uch a F11) or the Ctrl+M key
combination.
Example Cro -reference within the document or to other document .
Warning! It might cau e irrever ible damage or harm to the u er of the product or the
product it elf if thi in truction i not followed.
Caution! It might cau e the product to not work properly if thi in truction i not followed
and alert to a potential danger ituation.
Note: Call attention to ome important feature .
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Pannoramic SCAN 150 1.17 User’s Guide Character Formats and Symbols
Declaration of Conformity
3DHISTECH Ltd. declare that the product Pannoramic SCAN 150 lide canner i de igned and
produced with con ideration of pecified requirement according to the ISO 13485:2003 and IEC
61326-2-6:2005 tandard , and conform to the directive and other tandard li ted below:
IEC 61010-1:2001 (ed. 2), “Safety requirement for electrical equipment for mea urement, control,
and laboratory u e – Part 1: General requirement ”
IEC 61010-2-101:2002 (ed. 1), “Safety requirement for electrical equipment for mea urement,
control and laboratory u e – Part 2-101: Particular requirement for in vitro diagno tic (IVD) medical
equipment”
EN 55011:1998, Group 1, Cla A, “Limit and method of mea urement of radio di turbance
characteri tic of indu trial, cientific, and medical (ISM) radio-frequency equipment”
Thi product ha been te ted to the requirement of CAN/CSA-C22.2 No. 61010-1, econd
edition, including Amendment 1, or a later ver ion of the ame tandard incorporating the ame level
of te ting requirement .
Conformity with Directive 98/79/EC i marked by on the Pannoramic SCAN 150 device.
Conformity with Directive IEC 61010-2-101:2002 (ed. 1) i marked by on the product
labeling.
Further information may be obtained from the manufacturer:
3DHISTECH Ltd.
29-33 Konkoly-Thege Miklo treet, Building 6
1121 Budape t – HUNGARY
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Pannoramic SCAN 150 1.17 User’s Guide Notes Regarding Operational Safety
Notes Regarding Operational Safety
The below ection of thi U er’ Guide contain information and warning of a kind that mu t be
followed by owner/operator per onnel.
Warning and advi ory ymbol which are u ed on the ba e unit of the Pannoramic SCAN 150 and in
the ection 1.1.3 Warning and Information Labels have the following meaning :
CAUTION
Di connect main plug before any kind of intervention into the device!
CAUTION
Cru h hazard!
The Pannoramic SCAN 150, including any of it original acce ory component , hould not be
operated in any other way than de cribed in ection 1.1.1 Intended Use.
Any of the following note hould be trictly ob erved:
Caution!
In the event of any non-conforming type of u e, including non-conforming u e
of a emblie or ingle part , no liability will be a umed by the Manufacturer.
Thi hall al o apply to ervice or repair work of any kind, which i performed by
other than duly authorized ervice per onnel. All claim for warranty and
warranty ervice will be null and void in any uch ca e.
Warning!
In ert the power cord plug only into a grounding outlet. The protective action
mu t not be rendered ineffective by exten ion cable without protective
conductor. The Pannoramic SCAN 150 ha a built-in protection which mu t not
be rendered ineffective by exten ion cable without protective (earth-ground)
conductor.
Warning!
Never operate the device in explo ion-ri k environment.
Warning!
If protective device are found to be ineffective, the Pannoramic SCAN 150 mu t
be hut down and precaution mu t be taken to prevent unintended u age. For
nece ary repair work or work to re tore proper operating condition, you
hould contact the 3DHISTECH Cu tomer Service.
Note:
Electric power to the Pannoramic SCAN 150 i upplied via an external main
power upply unit. It allow for operation at line voltage in the range of 100 V
to 240 V ±10%, 50/60 Hz, without any extra facilitie required for voltage
tran formation.
Warning!
Check that your local line voltage i compatible with Pannoramic SCAN 150
pecification before you turn power on.
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Pannoramic SCAN 150 1.17 User’s Guide Notes Regarding Operational Safety
Disconnect plug!
Remember to unplug the power cord before you open the Pannoramic SCAN
150 or replace a fu e!
Warning!
Only connect external device that are afety extra low voltage rated to the
in trument to avoid the ri k of electrical hock.
Warning!
Pannoramic SCAN 150 doe not include any pecial facilitie to protect from
ample with an etching, potentially infectiou , toxic, radioactive or other
health-damaging effect. You are under obligation to comply with all currently
binding legi lation, notably, national accident prevention rule when handling
ample of thi kind.
Caution!
Dirt or du t may adver ely impact the operating capability of the Pannoramic
SCAN 150. For thi rea on, mea ure mu t be taken to eliminate uch influence
to a maximum po ible degree. During period of non-u e, the Pannoramic
SCAN 150 mu t be protected with a du t cover. Check that power i turned off
before you put the cover on.
Caution!
Avoid touching the lamp bulb. Di connect power and let the lamp cool down for
about 15 minute before replacing the lamp.
Crush hazard!
There i cru h hazard in the movement area of the lide in ert during operation.
Plea e note that the digitizing proce ha to be interrupted and the in ert
moved via oftware to the output po ition before removing the in ert.
Warning!
A defective Pannoramic SCAN 150 i not cla ified a dome tic wa te. It mu t be
properly di po ed in accordance with currently valid legal requirement .
Warning!
The Pannoramic SCAN 150 may not be operated by other than properly
in tructed per on . Operating per onnel mu t be fully aware of the potential
danger which a particular field of application involve . The Pannoramic SCAN
150 i a preci ion in trument which may uffer a ignificant reduction in
operational function or even phy ical de truction following intervention
performed in any nonconforming manner.
Warning!
If an X-Cite 120 illuminator i u ed, the corre ponding warning included in the
manufacturer’ operating manual have to be ob erved.
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Pannoramic SCAN 150 1.17 User’s Guide Notes on Warranty
Notes on Warranty
3DHISTECH Ltd. – a the Product Manufacturer – warrant the Pannoramic SCAN 150 to be free from
fault in material and workman hip at the moment of in tallation. Defect mu t be notified
immediately on identification and maximum effort mu t be undertaken in order to the amount of
damage a mall a po ible. On receiving notice of a fault, the Product Manufacturer will be under
obligation to remove the fault, at hi own option, either by providing adequate repair ervice or by
delivering a faultle product for replacement. No warranty will be accepted in ca e of natural wear
(notably, wear and tear part ) or improper product handling.
The Product Manufacturer will refu e any liability in the event of damage re ulting from faulty
operation, negligence or unauthorized intervention into the product, notably, from the replacement
of product part or the u age of acce ory part from other manufacturer . Any uch ca e will void
the u er’ claim for warranty.
Except for action which i explicitly de cribed in thi U er’ Guide, you are prohibited from
performing any kind of maintenance or repair work at the Pannoramic SCAN 150. Only 3DHISTECH
Service per onnel or pecifically authorized repre entative are allowed to handle repair work. In
ca e of malfunction, contact the 3DHISTECH Service Support fir t.
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Pannoramic SCAN 150 1.17 User’s Guide Terms and Abbreviations
Terms and A reviations
CSV Comma Separated Value
FOV Field of View
NA Numerical Aperture
Virtual Slide / Slide A digital image of a thin gla plate on which pecimen are mounted for
micro copic tudy:
a dynamic, interactive image that you can manage, ave, magnify, zoom, name,
evaluate, annotate, mark, comment, end it to a colleague electronically for co-
operation or advice, and o on.
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
1 Product Description
1.1 Product Overview
1.1.1 Intended Use
The Pannoramic SCAN 150 i de igned to u e tran mitted light and reflected light fluore cence to
digitize biological ample and pecimen (typically hi tological ection ). The target u er of the
product can work for example, in education, re earch, indu try or medical ector .
Warning!
After each canning make ure to in pect the digital lide for over-expo ed
area . If the lide i canned ba ed on improperly et parameter , it can
highly affect the diagno tic re ult becau e of information lo .
1.1.2 Features and Benefits
The Pannoramic SCAN 150 offer world cla brightfield and fluore cent whole lide canning on up
to 9 channel . An out tanding 0.2 µm/pixel re olution i achieved with the 40×/NA 0.95 (equivalent
to 50× magnification in BF and 62× magnification in FL) Carl Zei Plan-Apochromat objective.
Key feature of the Pannoramic SCAN 150 Scanner are:
•Up to 9 freely electable color filter for fluore cence
•High-end fluore cent illumination device – Lumencor Spectra Light Engine 6
•Multiband filter upport
•FL canning with a low noi e 16-bit cientific CMOS pco.edge camera
•New oftware-aimed flat-field correction compen ation method to create homogenou
lide image
•150 lide automatic loading and canning
•Improved permanent autofocu
•3MP camera for barcode reading and preview image
•Image harpening (for both Fluore cence and Brightfield) feature which improve the vi ual
appearance of digital lide
•Focu algorithm find the focu which i mo t appealing to the human eye
•Small footprint
•Compact and robu t device
•Good quality and table manufacturing
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
Pannoramic SCAN 150 i an optimal choice for cu tomer who need high quality daily throughput
granted by the loading capacity of 150 lide .
1.1.3 Warning and Information La els
The following figure contain the label u ed on the Pannoramic SCAN 150 main unit, the external
main power upply unit, and the HXP 120 illuminator.
Figure 1 – Warning and information labels
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
1.1.4 Base Unit
The following figure how the main part of the ba e unit.
Figure 2 – Main parts of base unit
1. Protective cover
2. Output tack for magazine
3. Input tack for magazine
4. Protective cover for magazine loader
5. Magazine feeder door/cover
6. CIS VCC-FC60FR19CL can camera
7. Safety rail
8. HXP 120 fiber-coupled illuminator (optional, u e with AxioCam MRm Rev3 camera only)
9. HXP 120 illumination adapter (optional, u e with AxioCam MRm Rev3 camera only)
10. Fiber optic cable
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
1.2 System Overview
The tandard cope of delivery of the product include the following hardware and oftware
component .
1.2.1 Pannoramic SCAN 150 system
The following figure how the main part of the Pannoramic SCAN 150 y tem.
Figure 3 – Pannoramic SCAN 15 system overview
1. HXP 120 fiber-coupled illuminator (option)
2. 24 V external power upply unit
3. Monitor
4. Mou e
5. Control computer
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
6. Keyboard
7. Pannoramic SCAN 150 ba e unit
8. Slide magazine with cover
1.2.2 Hardware Options
The configuration of the control computer and monitor can vary depending on the product order.
Cameras
AxioCam MRm Rev 3
1 CCD cientific, cooled, monochrome camera
for high quality, en itive fluore cent
application .
CIS VCC-FC60FR19CL
CMOS camera for high-quality brightfield
application allowing fa t canning.
pco.edge
Equipped with a high-quality CMOS image
en or for fluore cent application allowing fa t
canning and an out tanding low read out noi e.
Cooled with Peltier element.
Stingray F146C IRF Medical camera
1 CCD camera for normal brightfield application
and entry level fluore cent application .
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
Camera adapters
0.63× adapter 1× adapter 1.6× adapter
Warning!
The in tallation of the camera in Pannoramic SCAN 150 mu t be
carried out by trained profe ional !
In any other ca e the warranty will be automatically voided. The
upplier or di tributor i not liable for any damage cau ed by an
in tallation carried out by an unprofe ional per on.
Base Unit options
•Pannoramic SCAN 150 Ba ic Scan Option / Fa t Option – Ba e unit equipped with Stingray or
CIS camera for Brightfield canning (no fluore cent acce orie included)
•Pannoramic SCAN 150 Ba ic Fluore cence Option – Ba e unit completely equipped with
AxioCam camera and HXP 120 illuminator, trigger cable and filter for fluore cence canning
•Pannoramic SCAN 150 Advanced Fluore cence Option – Ba e unit completely equipped with
pco.edge camera and Lumencor fluore cence illuminator, trigger cable and filter for
fluore cence canning
•Pannoramic SCAN 150 Ba ic Mixed Option – Ba e unit completely equipped with Stingray or
CIS VCC-FC60FR19CL camera for Brightfield canning; with AxioCam camera and HXP 120
illuminator, trigger cable and filter for fluore cence canning
•Pannoramic SCAN 150 Advanced Mixed Option – Ba e unit completely equipped with CIS
VCC-FC60FR19CL camera for Brightfield canning, and with pco.edge camera and Lumencor
fluore cence illuminator, trigger cable and filter for fluore cence canning
NOTE: In ca e Lumencor light ource i ordered, the cu tom light ource and filter module
can be variable. For more information, plea e contact 3DHISTECH cu tomer ervice.
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
O jectives
•Carl Zei Plan-Apochromat 20×/NA 0.8
•Carl Zei Plan-Apochromat 40× Corr/NA 0.95
Warning!
Do not change correction collar etting on the 40×/NA objective, if
you need it to be et, contact cu tomer ervice.
Fluorescent Options for Pannoramic SCAN 150
Fluore cent unit (internal part)
HXP 120 Fluore cent illumination unit
(can be u ed with AxioCam MRm
Rev3 and Stingray camera only)
Lumencor Spectra Light Engine 6
illumination unit (can be u ed with
pco.edge camera only)
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Pannoramic SCAN 150 1.17 User’s Guide 1 Product Description
1.2.3 Control Software
Pannoramic Scanner control software
Optional:
•Fluore cence
•Extended focu
•Z- tack
•Barcode reader (licen ed feature)
•Scan To Server
Pannoramic Viewer software (fully functional)
Optional:
•Ca eCenter
•Pannoramic Viewer TMA module
•3D Recon truction module
•Hi toQuant, NuclearQuant, MembraneQuant, FISHQuant, and Den itoQuant module
•ImmunoScreener module
•E-School Encoder
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