3D Systems Allevi 3 User manual

Allevi 3
User Guide - 1.1.2

BUILD WITH LIFE

SETTING UP YOUR PRINTER 11
Table of Contents
WELCOME
SPECIFICATIONS
WARNINGS
3
4
5
GETTING STARTED
WHAT`S IN THE BOX
PRINT BED DIMENSIONS
ALLEVI 3 DIAGRAM
7
8
9
10
SETTING UP YOUR SOFTWARE
CONNECTING TO YOUR PRINTER
13
15
START PRINTING
YOUR FIRST BIOPRINT
17
18
MAINTENANCE & TROUBLESHOOTING
TROUBLESHOOTING AND CONTACT US
24
25

WELCOME

SPECIFICATIONS
MECHANICAL
Construction:Aluminum Frame
Build Surface: Tissue Culture Plates
XYZ Position Precision: 1 micron
Weight: 40 lbs (18.1 kg)
Dimensions: 18.4 x 15.2 x 14.2 in
46.7 x 38.8 x 36.0 cm
ELECTRICAL
Power/Voltage Requirements: 6 Amp/
AC 110V - 220V
Desktop Adaptor
Supply Voltage: 24V DC, 16.7A
SOFTWARE
Bundle: Allevi Bioprint Online
Connectivity: WiFi, Ethernet,
Allevi Wireless Adapter
Supported File Types: STL, gcode
Operating System: Windows, MacOSX, Linux
PRINTING
Print Technology: Extrusion Printing
Pressure Range: 1- 120 PSI (7- 827 kPa)
Pressure Variance: +/- 0.1 PSI
Build Volume: 3.5 x 2.4 x 5.1 in (42.8 in³)
9 x 6 x 13 cm (702 cm³)
CORE PRINTHEAD
Max Temp (E1): 160ºC
Min Temp: 4ºC
LED Range: 365 nm and 405 nm
BUILD PLATE
Max Temp: 60ºC
Min Temp: Room temperature
4
Allevi 3 | User Guide

HOT SURFACE WARNING
This symbol indicates potential hot surfaces. The Allevi 3 and its extruder(s)
can become hot during the printing process, whether heating or cooling.
Always allow time for the extruder to cool before handling.
WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT
This symbol indicates that when the end-user wishes to discard this product, it
must be sent to separate collection facilities for recovery and recycling.
UV WARNING
This symbol indicates potential ultraviolet light exposure. The Allevi 3’s extru-
der(s) can emit wavelengths of 365-405nm. Wear UV glasses when operating
and avoid eye and skin exposure to unshielded product.
CE MARKING
This mark indicates conformity with health, safety, and environmental protec-
tion standards for products sold within the European Economic Area.
SGS MARKING
This mark means a product has been independently tested and certified to
meet recognized standards for safety or performance.
WARNINGS
5
Allevi 3 | User Guide

•Indoor use
•Altitude ofoperation: <2000m
•Operating temperature: 5- 40ºC
•Relative humidity: 20-80% (up to 31ºC), decreasing linearly to 50% at 40ºC
•Pollution degree of the environment: Pollution degree 2
The Allevi 3 should be stored in the following conditions:
•Temperature: 5- 40ºC
•Humidity: 20- 80% (up to 31ºC), decreasing linearly to 50% at 40ºC
•WARNINGS
•The Allevi3includes moving parts that can cause injury.Never reach inside theprinter while it is
in operation.
•Do not leave the printer unattended while it is in operation.
•Always wear safety goggles when using the printer.
•Do not mount the Allevi 3 on or near a surface of flammable material.
•There are possible hazards of explosion, implosion, or the release of toxic or flammable gases
arising from materials being heated.
•If you are working with hazardous materials, you should set up theappropriate extraction system
and/or ventilation requirements.
•You are responsible for appropriate decontamination in case of spillage of hazardous materials
on or inside ofthe equipment.
•If the equipment is used in amanner not specified by Allevi, the protection provided by the equip-
mentmay beimpaired.
6
Allevi 3 | User Guide
ENVIRONMENTAL CONDITIONS
The Allevi 3 should be operated in the following conditions:
MAINTENANCE AND CLEANING INSTRUCTIONS
•Recommended decontamination is spraying with 70% ethanol while the printer is powered down,
turning your hood fan on for 30 minutes, and wiping down the printer after at least 10 minutes.
Additionally, 30 minutes of UV decontamination may be used to supplement the ethanol wipe down.
•Do not over-spray ethanol directly into the electronics of the Allevi 3. Spray a towel, then wipe the
printer down with the towel. Do not use decontamination or cleaning agents which could cause a
hazard as a result of a reaction with parts of the equipment or with material contained in it.
SERVICE INSTRUCTIONS
Allevi bioprinters should be serviced by Allevi or Allevi-certified personnel.
To request servicing, email support.all[email protected]

GETTING STARTED

8
Allevi 3 | User Guide
WHAT’S IN THE BOX
Allevi 3 bioprinter
1Starter bioink kit
2
Power supply
3Air compressor*4Compressor hose5
*Air compressor arrives separately

28mm
77.50mm
Diameter
88.30mm
9
Allevi 3 | User Guide
PRINT BED DIMENSIONS

A. Air Compressor Lines
B. CORE™ printhead (heating and cooling)
C. Autocalibration pad
D. Build Plate (heating)
E. Power On/Off
C
A
B
D
E
10
Allevi 3 | User Guide
ALLEVI 3 DIAGRAM

11
Allevi 3 | User Guide
SETTING UP YOUR PRINTER
Step 1
Carefully remove your printer from the packaging by grasping the printer frame firmly with both
hands. We recommend holding onto the packaging for future moving or shipping the printer.
CAUTION: The Allevi 3 weighs ~ 40 lbs (18.1 kg). Two people may be required to safely lift it.
Always carry the printer by the frame/bottom.
Step 2
Once the printer is securely placed on a flat surface, connect the CORE extruders to the gantry.
Each CORE extruder is magnetized so each will snap into place.
Note: You can operate the printer with the CORE print heads in many different configurations. We will
discuss further in "Your First Bioprint" later in this manual.
Step 3
Connect the red plastic air hoses to the top of the frame, and the air input junction to the port on
the back of the frame.
Step 4
Plug the power cable into the power port located on the right side of the back of the bioprinter.
Then plug the other side into a wall outlet.
3.2.

12
Allevi 3 | User Guide
Step 5
Power on the bioprinter by pressing the power button located on front of the bioprinter. It will turn
red.
Step 6
Connect the air compressor to the black air hose, and the air hose to the air input junction on the
printer. The connection between the hose and the junction can be tricky. Follow this diagram:
AGather both the air input junction (left) and the air hose (right). BPull back the collar of the air
hose. CInsert the air input junction in the air hose. DSlide the collar of the air hose back into place.
EThe connection should be formed! FGive the connection a light tug to ensure it is secure.
Note: The air compressor must remain turned on for the entirety of the print.
Using the knob near the outlet valve, set the pressure on both gauges to 120 PSI. This will ensure you have
plenty of pressure to use during printing. Any adjustment to your printing pressure is made in the software,
so leave the air compressor on this setting. After use, turn the air compressor off.
Note: The air compressor needs to be periodically drained to eliminate any condensation inside. To do
so, refer to the air compressor manufacturer manual. This should be done following every day of use.
Please refer to the air compressor manufacturer manual for all preventative maintenance.
C
DEF
AB
5.4.

SETTING UP YOUR SOFTWARE
13
Allevi 3 | User Guide
You can also follow a video at allevi3d.com/getting-started
Step 1
Go to bioprint.allevi3d.com. Our software runs out of a browser and requires no downloads.
We recommend Google Chrome or Firefox, but Safari and Edge are also acceptable.
Step 2
Login to the software. To set up an account, click ‘Create an account’.
Step 3
To register your printer, scroll down in your printer list and click ‘Add printer’. If you’re planning on
having multiple users work with your printer, we would recommend that you first make a Team and
add the printer to your Team. A guide to do this can be found here.

14
Allevi 3 | User Guide
Step 4
Type in your device serial number and click ‘Add printer’. Your serial number can be found
on the back of your printer or in your onboarding email from the Allevi Support Team.
Note: The serial number has 7 characters and should be limited to letters between a-f and numbers.
Step 5
Once your printer is registered, it will appear as an available printer in your printer list.

Ethernet connection:
To connect your Allevi bioprinter via ethernet, plug an ethernet cord to the port on the back of the
printer and to an ethernet port in your lab. You need to power cycle with the ethernet connected.
Wi-Fi Connection:
With a Wi-Fi connection, your printer will be online.
Step 1
Open your WiFi settings and look for a network called “Allevi Printer <serial number>.
By connecting to the Allevi hotspot, your computer will briefly lose access to the Internet.
Step 2
Go to http://10.42.0.1/ in your browser.
Step 3
Click Configure under ‘Wifi Settings’ and enter your network credentials on the next screen. The
chosen WiFi network should be a private network without a ‘Click to accept’ intermediate page.
Once the network has been successfully added, a green message will pop up at the bottom of the
page. You can now close this window.
Adapter Connection:
With this connection method, your Allevi bioprinter will not be connected to the internet itself, but
it will communicate with your computer via the Allevi Adapter.
Step 1
Download the official driver here. Extract the downloaded .zip file and run the installer. This allows
your computer to connect to two Wi-Fi networks at the same time.
15
Allevi 3 | User Guide
CONNECTING TO YOUR PRINTER

Download the Allevi Client app here. Extract the downloaded .zip and run the application.
You’ll need to keep this open whenever you are using your Allevi bioprinter.
Step 2
Plug your Allevi Adapter into your computer’s USB port.
Step 3
Turn your printer on.
Step 4
In your Wi-Fi list, you should see a dropdown to select different Wi-Fi networks. The dropdown
allows you to switch between “Wi-Fi 1” and “Wi-Fi 2”; however on some computers this may be
called “Wi-Fi 2” and “Wi-Fi 3”. See the screenshot below for an example. It does not matter what
your networks are called, as long as they are connected as follows:
•Wi-Fi 1: Connect this to your home or work Wi-Fi network
•Wi-Fi 2: Connect this to the Allevi Printer Wi-Fi Access Point “Allevi Printer <serial number>”
Step 5
Navigate to https://bioprint.allevi.app and log in as normal using your Bioprint credentials.
Step 6
On your printer dashboard, turn off your printer’s Cloud Mode using the cloud icon and follow the
system prompts. This will take 3-5 minutes to complete.
Step 7
If connection is successful, you will see the following state on the Allevi Client Application and a
green Connect button in the printer dashboard.
16
Allevi 1 | User Guide

START PRINTING

18
Allevi 3 | User Guide
YOUR FIRST BIOPRINT
The Allevi Bioprint Online software is the most comprehensive and versatile bioprinting software.
If you prefer watching videos, visit allevi3d.com/getting-started and watch the ‘Your First Bioprint’
video.
Step 1
Store your jar of sample pluronic at 4° C until the pluronic is easily pourable.
a) To load your syringe, remove the plunger from a printing syringe. Detach the rubber seal from
the plunger and set it aside.
b) Add viscous biomaterial to the wide opening of the syringe barrel. If your material comes in a
tube or another syringe, squeeze it out directly. You can also use a spatula to place some material
in the barrel.
c) Place the rubber seal in the syringe barrel.
d) Use the plunger to push the rubber seal to move the material all the way to the tip of the syringe.
Invert the syringe and push the plunger pointing the syringe upwards. This can also help reducing
bubbles.
Note: If you are using the syringe plunger, make sure it doesn’t lock back into the rubber seal.
Note: Bioinks may be loaded differently and use different needles depending on the type of material.
View our General Bioink Parameters guide on Allevi Support.
Step 2
Remove the middle and right-most CORE extruders from the gantry. This print will only utilize one
extruder so we only need the left-most extruder. In future prints, you will be required to calibrate
all connected extruders, so we recommend only having as many extruders attached as you want
to use.

Step 3
Attach a 30 gauge 0.25” (pink) needle to the pluronic syringe, and load syringe into the CORE print-
head. Twist the cap under the shoulder screws to secure it. Make sure to press the syringe and the
extruder together as they twist the cap under the shoulder screws.
Step 4
Turn on your printer. Yellow lights will stay on while the printer loads its firmware and connects to
the Internet. You can connect to your printer once the yellow lights are off.
Step 5
Login to bioprint.allevi3d.com. Select your printer from the printer list and click ‘Connect’.
Step 6
This will bring you to your printer Status section. Here you can get an overview of your print
settings. We recommend always autocalibrating first.
Always autocalibrate with a syringe and needle loaded in the printhead. Do NOT autocalibrate with a
dish on the bedplate.
19
Allevi 3 | User Guide