3M Attest Auto-reader 490 User manual

Description:
DIELINE
Base File Name:
Software:
SGS Contact:
Email:
Printer:
Printer Location:
Supplier:
3M™ Attest™
Auto-Reader 490
Requester: Doug Wynveen
Reference:
Die # / Doc. Size: 8.5" x 11"
Item Spec#: 34-8723-2501-3
Supersedes#: 34-8721-7544-2
Cat/Product#: 490
Structure#: SS-51660
Cover and Operator’s Manual
PA: 34872325013.indb
GA:
InDesign CC
PROCESS
BLACK
PROCESS
CYAN
3M
RED
PROCESS
MAGENTA
PROCESS
YELLOW
5324303 - 4 2
06.10.19 PU files from OMNI and check for style and structure. kat
06.20.19 Final Release kat
3M™Attest™Auto-reader 490
1Operator’s Manual
2Manuel de l’utilisateur
3Benutzerhandbuch
4Manuale dell’operatore
5Manual del usuario
5Manual del operador
6Bedieningshandleiding
7Användarhandbok
8Brugsvejledning
9Brukerhåndbok
0Käyttöopas
-Manual do Utilizador
-Manual do Operador
=Εγχειρίδιο Χειριστή
HInstrukcja obsługi
FPříručka pro obsluhu
SPriročnik za uporabo
MИнструкция по эксплуатации
]Upute za korištenje
dOperatör Kılavuzu
I󱯿󰚎󱨹󰪉
j
T󺇸󺀬󺇷 󹑋󸳛󺀣

1
1
3MAttestAutoreader490 1
Read and understand all safety information before using this product.
TABLE OF CONTENTS
PREFACE.............................................................................................................................................................................. 2
INTENDED USE..................................................................................................................................................................... 2
INDICATIONS FOR USE ........................................................................................................................................................ 2
WARRANTY.......................................................................................................................................................................... 2
SAFETY AND PRECAUTIONS ............................................................................................................................................... 2
HEALTH AND SAFETY INFORMATION.................................................................................................................................. 3
EXPLANATION OF PRODUCT ANDPACKAGINGLABELSYMBOLS ..................................................................................... 3
SPECIFICATIONS.................................................................................................................................................................. 4
INSTRUCTIONS FOR USE..................................................................................................................................................... 4
FEATURES OF THE 3MATTESTAUTOREADER 490................................................................................................... 8
CARE AND CLEANING.......................................................................................................................................................... 8
TROUBLESHOOTING GUIDE ................................................................................................................................................. 9
OPTIONAL 3MATTESTAUTOREADER 490 WEB APP.............................................................................................. 10
TROUBLESHOOTING THE 3MATTESTAUTOREADER 490 WEB APP....................................................................... 14
REPAIR AND REPLACEMENT.............................................................................................................................................14

2 3
1
PREFACE
Manual Revision History
Date Revision Reason For Revision
SEP 2010 A Initial Issue
FEB 2012 B To include the incubation of 3M™ Attest™ Super Rapid
Readout Biological Indicators 1492V in the 3M™ Attest™
Autoreader 490 and add statements clarifying access
to the 3M™ Attest™ Autoreader 490 Web App within a
secure network.
JUN 2013 C To revise: the Warranty statement; and the WEEE and
CTick symbols and associated explanatory text.
SEP 2017 D This manual is applicable to 3M™ Attest™
Auto-readers 490 having software version 4.0.0 or
greater and a black incubation well configuration
sticker. Revised to: reflect reduced time for final
fluorescent result; add various compliance revisions; and
add rows for secondary DNS and MAC address to 490 Web
App Configuration Page.
FEB 2018 E Manual revised to add: incubation of Attest™ Rapid
Readout Biological Indicators for Vaporized Hydrogen
Peroxide Sterilization 1295 in 3M™ Attest™ Auto-readers
490 having software version 4.0.0 or greater; and option
to disable the 490 Web App.
AUG 2018 F Manual revised to add Date of Manufacture symbol and
correct typos.
Content Disclaimers
Pictorial Disclaimer
Sample printouts, graphics, displays and screens are for information and illustration
purposes only, and shall not be used for clinical or maintenance evaluations. Data shown
in sample printouts and screens do not reflect actual patient names or test results.
Hardware Disclaimer
The 3M™ Attest™ Autoreader 490 hardware specifications are subject to change.
The system images, hardware components and hardware specifications included in
the manual may not match the system installed. Any changes or modifications to the
authorized system installation have been verified as compatible with the functionality
outlined in this document.
INTENDED USE
The 3M™ Attest™ Super Rapid Readout Biological Indicator System provides rapid,
reliable monitoring of steam sterilization processes performed in health care facilities.
The system is comprised of biological indicators, challenge packs, and incubator/readers
(3M™ Attest™ Autoreader 490 and 3M Attest™ Autoreader 490H having software
version 4.0.0 or greater) with embedded software.
The 3M™ Attest™ Autoreader 490 having software version 4.0.0 or greater is
designed to incubate and automatically read 3M™ Attest™ Super Rapid Readout
Biological Indicators 1491 and 1492V at 60°C for a final negative fluorescence reading
at 24minutes. The 3M™ Attest™ Autoreader 490 is also designed to allow for further
incubation of 3M™ Attest™ Super Rapid Readout Biological Indicators, catalog numbers
1491 and 1492V, for a final negative, visual pH color change result at 24 hours and 48
hours, respectively. This further incubation is optional and not intended for routine use.
3M™ Attest™ Auto-readers 490 having software version 4.0.0 or greater may also
be used as part of the 3M™ Attest™ Rapid Readout Biological Indicator System for
Vaporized Hydrogen Peroxide Sterilization to provide rapid and reliable routine monitoring
of vaporized hydrogen peroxide sterilization processes in health care facilities. This system
is comprised of incubator/readers (3M™ Attest™ Auto-reader 490 having software
version 4.0.0 or greater and 3M Attest™ Auto-reader 490H) with embedded software,
and a biological indicator, the 3M™ Attest™ Rapid Readout Biological Indicator 1295.The
3M™ Attest™ Auto-reader 490 having software version 4.0.0 or greater is designed to
incubate and automatically read 3M™ Attest™ Rapid Readout Biological Indicators 1295
at 60°C for a final negative fluorescence reading at 24 minutes.
A positive fluorescence reading or a visual pH color change indicate a sterilization process
failure. However, due to the high sensitivity of the fluorescent result, there is no advantage
to continuing to incubate processed 3M™ Attest™ Biological Indicators once the
fluorescent result has been documented.
Other biological indicators (BIs) are not compatible with this device and cannot be used.
INDICATIONS FOR USE
The 3M™ Attest™ Auto reader 490is designed to incubate and automatically read
3M™ Attest™ Rapid Readout Biological Indicators 1295 and 3M™ Attest™ Super
Rapid Readout Biological Indicators, catalog numbers 1491 and 1492V, at 60°C for a final
fluorescent result at 24minutes.
WARRANTY
In the US, the 3M™ Attest™ Autoreader 490 has a one year limited product warranty.
The US warranty, remedy and limitations are described in the Price Quote form and in
the Direct Price pages. For countries outside of the US, any warranty is established by the
subsidiary with responsibility for service of the 3M™ Attest™ Autoreader 490.
Serial Number
For easy identification, each 3M™ Attest™ Autoreader 490 has a unique serial
number printed on a label found on the back of the unit and displayed on the
left side of the upper row of text on the LCD Display when the user depresses
the button. Please record your serial number in this manual for future
reference: ________________________________.
SAFETY AND PRECAUTIONS
The 3M™ Attest™ Auto-reader 490 and its related devices and accessories are designed
to provide safe and reliable service when used according to the instructions provided.
Please read, understand, and follow all safety information contained in the instructions for
use included with the 3M™ Attest™ Auto-reader 490, the 3M™ Attest™ Rapid Readout
Biological Indicator 1295, and 3M™ Attest™ Super Rapid Readout Biological Indicators
1491 and 1492V devices prior to use. Use this equipment only for the purpose described
in the Operators Manual. Retain these instructions for future reference.
The unit is designed to be used only with the Power Supply module and Ethernet cable
supplied by 3M. If this product is used in a manner not specified, the protection provided
by the product may be impaired.
Explanation of Signal Word Consequences
WARNING: Indicates a hazardous situation, which, if not avoided, could result in
death or seriousinjury.
CAUTION: Indicates a hazardous situation, which, if not avoided, could result in
minor or moderateinjury.
Warnings and Safety Precautions
The following warnings and precautions should be followed to avoid unsafe actions that
could result in personal injury or damage to theinstrument.
WARNING: To reduce the risk associated with hazardous voltage
Use indoorsonly.
Do not use the equipment if it is not working properly or if it has suffered anydamage.
Use only the power supply specified for this product and certified for the country
ofuse.

2 3
1
CAUTION: To reduce the risk of injury or instrumentdamage
Do not spill liquid onto or into the instrument. Do not immerse the unit inliquid.
Always unplug the 3M™ Attest™ Autoreader490 and allow to cool before cleaning.
Clean external surfaces using only the instructions provided by themanufacturer.
Do not open the instrument housing there are no user serviceableparts. The
instrument must be returned to the manufacturer forrepair.
Wear safety glasses and gloves when activating the 3M™ Attest™ Rapid Readout
Biological Indicator 1295.
Allow the 3M™ Attest™ Super Rapid Readout Biological Indicators 1491 and 1492V
to cool for the recommended time period before activating. Activating or excessive
handling of the biological indicator before cooling may cause the glass ampoule
toburst.
Wear safety glasses when activating the 3M™ Attest™ Super Rapid Readout
Biological Indicators 1491 or 1492V.
WARNING: To reduce the risk associated with incorrectresults
Instrument is to be used by operators familiar with the device, device functionality,
andOperators Manual.
Do not place the instrument in environment exposed to sunlight or strong
incandescentlight.
Do not place instrument close to any device that emits a strong electromagneticfield.
Do not use on a vibratingsurface.
Activate and incubate 3M™ Attest™ Rapid Readout Biological Indicators 1295 within
1 hour of the completion of the sterilization cycle.
Do not incubate a 3M™ Attest™ Biological Indicator if, after processing and before BI
activation, it is observed to have a broken media ampoule. Retest the sterilizer with a
new biological indicator.
Allow 3M™ Attest™ Super Rapid Readout Biological Indicators 1491 and 1492V to
cool for the recommended time period before activating.
Do not remove or change placement of 3M™ Attest™ Biological Indicator once it is
placed into a well.
Do not remove the 3M™ Attest™ Biological Indicator from the incubation well until the
(+)or() symbol on the LCD panel indicates the test iscomplete.
CAUTION: To reduce the risk associated with incorrectresults
To avoid the possibility of the 3M™ Attest™ Biological Indicator vial
absorbing fluorescent residue from a chemical indicator or tape, place the
3M™ Attest™Biological Indicator vial so it does not come in direct contact with
chemical indicators ortape.
HEALTH AND SAFETY INFORMATION
Device Safety Compliance
The 3M™ Attest™ Autoreader 490 complies with the following standards as
demonstrated by the CB Scheme Certificate and test report issued by Underwriters
Laboratories (UL):
• IEC 610101 (2010) 3rd Edition. Safety requirements for electrical equipment for
measurement, control, and laboratory use Part 1: General requirements
• IEC 610102010:2014 3rd Edition. Safety requirements for electrical equipment for
measurement, control, and laboratory use Part 2010: Particular requirements for
laboratory equipment for the heating of materials.
The 3M™ Attest™ Autoreader 490 is listed as Laboratory equipment and carries the
UL mark with adjacent indicators Cand USbased on compliance to the standards UL
610101, CAN/CSA 22.2 No. 610101 and CAN/CSA 22.2 No. 610102010.
The 3M™ Attest™ Autoreader 490 complies with the CE mark related to the Low Voltage
Directive (LVD) 2006/95/EC as confirmed in the Declaration of Conformity.
The 3M™ Attest™ Autoreader 490 complies with the RoHS Directive, Directive 2011/65/
EU of the European Parliament and of the Council of 8 June 2011 on the restriction of the
use of certain hazardous substances in electrical and electronic equipment.
The 3M™ Attest™ Autoreader 490 complies with the WEEE Directive, Directive 2012/19/
EU of the European Parliament and of the Council of 04 July 2012 on waste electrical and
electronic equipment (WEEE).
EMC Compliance
The 3M™ Attest™ Autoreader 490 complies with the following EMC standards as
confirmed in the Certificate of Compliance generated by 3M:
• IEC 613261:2012/EN 61326:2013 Electrical equipment for measurement, control and
laboratory use EMC requirements Part 1: General requirements
• EMC requirements of the CE mark EMC Directive 2004/108/EC.
The 3M™ Attest™ Autoreader 490 complies with the Australian and New Zealand
electrical safety and electromagnetic compatibility requirements as confirmed in the
Suppliers Declaration of Conformity that is linked to the Australian/New Zealand RCM
(Regulatory Compliance Mark).
Note: This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15, Subpart B, of the FCC Rules. These limits are designed
to provide a reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, and can radiate radio
frequency energy and, if not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications. Operation of this equipment
in a residential area is likely to cause harmful interference in which case the user will be
required to correct the interference at their own expense.
This Class A digital apparatus meets all requirements of the Canadian
InterferenceCausing Equipment Regulations.
EXPLANATION OF PRODUCT
ANDPACKAGINGLABELSYMBOLS
Caution, see instructions for use
Li
Waste Electrical and Electronic Equipment (WEEE) and EU Battery
Directive. This symbol indicates that both the device and the lithium
metal battery contained therein need to be disposed of properly.
UL Listed to US and Canadian SafetyStandards
Mark of Conformity to EuropeanDirectives
Direct current
Compliant to all applicable ACMA regulatory arrangements (RCM)
Manufacturer
Date of Manufacture
Catalog Number
Authorized representative for the European Community
SerialNumber

4 5
1
SPECIFICATIONS
Power Supply Specifications
Power supply Operating Condition Units
Voltage Range 100240 Volts AC
Frequency 50/60 Hertz
Current 0.6 Amps
Environmental Operating Conditions
Environmental Condition Operating Condition Units
Altitude 3000 (max) Meters
Operating Temperature 16 40 ºC
Storage Temperature 10 to 50 ºC
Operating Relative Humidity 2080 (noncondensing) %
Voltage Range 12 Volts DC
Frequency DC
Current 1.2 Amps
Installation/Over Voltage Category II
Pollution Degree 2
INSTRUCTIONS FOR USE Initial Setup
ChooseLocation
Place 3M™ Attest™ Autoreader 490 on a firm level surface away from sunlight and
incandescent light. To save counter space, units may be stacked two high.
Plug In Unit
Connect the power cord to the power supply. Connect the power supply to the 3M™
Attest™ Auto-reader 490 and plug the power cord into an AC grounded outlet.
WARNING:
• Do not place the instrument in environment exposed to sunlight or strong
incandescentlight.
• Do not place instrument close to any device that emits a strong
electromagneticfield.
• Do not use on a vibrating surface.
Wait 30Minutes BeforeUsing
Once plugged in, allow a 30 minute warmup period before placing biological indicators
into the incubation wells. The C1 caution code will disappear when proper incubation
temperature is reached.
SpecialConsiderations
It is recommended that the unit be left on to eliminate warm-up periods. The unit
may be turned off when it is anticipated that it will not be used for an extended period.
WARNING: Use only the power supply specified for this product and certified for
the country of use.

4 5
1
INSTRUCTIONS FOR USE Wells, Buttons, & Display Interpretation
1
11
9
4
7
8
2
3
5
10
6
Please familiarize yourself with the face of the 3M™ Attest™ Autoreader490:
Wells
1. Incubation/Reader Wells The 3M™ Attest™ Autoreader490 has ten
incubationwells.
Buttons
2. Information / Up Arrow Button
3. Temperature / Down Arrow Button
4. Buzzer Alarm OFF / Enter Button
Information Display
5. LCD Display Display is backlit.
6. LCD Display Example of Error Message for well #6
7. LCD Display Example of Remaining Incubation Time for well #10
8. LCD Display Example of Negative () BI result for well #9
9. LCD Display Example of Positive (+) BI result for well #8
10. LCD Display Alarm Enabled Indicator
Configuration Indicator
11. Incubation well configuration sticker is black.
INSTRUCTIONS FOR USE 3MAttest
Autoreader490Configuration
Note: Ideally, the 3M™ Attest™ Autoreader 490 should be empty (i.e. have no incubating
BIs) when it is reconfigured.
Press the and buttons on the front of the 3M™ Attest™ Autoreader490
simultaneously for 3 seconds to enter configuration mode. Once in configuration mode,
use the Up and Down buttons to set the 3M™ Attest™ Autoreader 490 clock,
audible alarm, and Web App as desired. The button acts as an Enterkey and moves
the flashing cursor to the next changeablesetting.
Setting the 3MAttestAutoreader490 Clock
The 3M™ Attest™ Autoreader490 date and time are displayed in this format:
YYYYMMDD HH:MM, with the time displayed using a 24-hour clock.To change the year,
if necessary, push the up or down buttons to set desired year, then press to advance
to the month, adjust as necessary and press to advance to the day. Use the
button to move to the time and set the hours andminutes.
Setting the Audible Alarm Function
Move across the top row of text by pushing the button. The flashing Bin Buzzer
indicates this is a changeable setting. It is recommended this be set at Buzzer Enabled
to audibly alert the operator to a positive BI but it may be set to Buzzer Disabledby using
either the up or down button.
Note: If Buzzer Disabled is selected, and the change accepted, the speaker icon ( ) will
disappear from the LCD panel and the alarm will be disabled for all positive 3M™ Attest™
Biological Indicators, error codes, and caution code alarmwarnings.
Note:The 3M™ Attest™ Autoreader 490 Display backlight flashes whenever an
audible alarm sounds. Disabling the audible alarm does NOT disable the LCD display
backlightflashing.
Press to select the desired option and advance to the next changeable setting.
Setting the Optional 3MAttestAuto-reader 490
Web AppFunction
It is recommended that the default Web Pages Enabled setting is used. If desired,
however, the 3M™ Attest™ Auto-reader 490 Web App can be disabled by pressing
either the up or down button. Press to select the desired option and advance to the
Acceptscreen.
Note: Disabling the 3M™ Attest™ Auto-reader 490 Web App will not affect connectivity to
3rd party software.

6 7
1
Accept Screen
Use the up or down buttons to toggle between Yes and No, press to accept changes
(Yes selected) or retain current settings (No selected) and exit the configuration mode. The
LCD panel will return to the standard display.
Note: Incubation wells on 3M™ Attest™ Autoreaders 490 having software version 4.0.0
or greater are not configurable. Contact 3M for instructions on configuring incubation wells
of Autoreaders having software versions less than 4.0.0.
INSTRUCTIONS FOR USE Readout Procedure
Instructions for Biological Indicator (BI) Activation andIncubation
CAUTION: Allow the 3M™ Attest™ Super Rapid Readout Biological Indicator
to cool for the recommended time period before activating. Activating or excessive
handling of the biological indicator before cooling may cause the glass ampoule
toburst.
CAUTION: To avoid the possibility of the 3M™ Attest™ Biological Indicator
vial absorbing fluorescent residue from a chemical indicator or tape, place the
3M™ Attest™ Biological Indicator vial so it does not come in direct contact with
chemical indicators or tape.
CAUTION: Wear safety glasses when activating the 3M™ Attest™ Super Rapid
Readout Biological Indicator.
CAUTION: Wear safety glasses and gloves when activating the 3M™ Attest™
Rapid Readout Biological Indicator 1295.
WARNING: To reduce the risk associated with incorrect results
Do not incubate a 3M™ Attest™ Biological Indicator if, after processing and before BI
activation, it is observed to have a broken media ampoule. Retest the sterilizer with a
new biological indicator.
Activate and incubate 3M™ Attest™ Rapid Readout Biological Indicators 1295 within
1 hour of the completion of the sterilization cycle.
Allow 3M™ Attest™ Super Rapid Readout Biological Indicators 1491 and 1492V to
cool for the recommended time period before activating.
Incubate the 3M™ Attest™ Rapid Readout Biological Indicator 1295 only in 3M™
Attest™ Auto-readers 490 having software versions 4.0.0 or greater.
Follow this procedure for every 3M™ Attest™ Biological Indicator to be read:
While wearing safety glasses (and gloves for the 1295
BI), PLACE BI in Attest™ Biological Indicator Activator.
CLOSE and SQUEEZE Attest™ Biological Indicator
Activator to close BI cap and crush media ampoule.
Immediately remove BI, visually VERIFY the glass
ampoule (containing growth media) has been crushed,
and FLICK BI to ensure purple growth media flows to the
growth chamber at the bottom of the vial.
PLACE the activated BI into any 3M™ Attest™
Autoreader 490 well and observe 3M™ Attest™
Autoreader 490 LCD panel to confirm correct placement
of the BI. If the BI is correctly seated, the 3M™ Attest™
Autoreader 490 LCD panel will display ***, **, * and
then remaining minutes of incubation directly below the
incubating BI.
RECORD BI result.
WARNING: Do not remove the biological indicator from the incubation well until
the (+) or () symbol on the LCD panel indicates the test is complete.
INSTRUCTIONS FOR USE Additional Information
BI Placement
All incubation wells in 3M™ Attest™ Autoreaders 490 having software version 4.0.0
or greater are programmed to provide a final fluorescent result within 24 minutes of
incubation and may be used to incubate both 3M™ Attest™ Super Rapid Readout
Biological Indicators 1491 and 1492V and the 3M™ Attest™ Rapid Readout Biological
Indicator 1295.
Remaining incubation time, in minutes, will be displayed on the LCD display to indicate
the 3M™ Attest™ Biological Indicator is incubating in the reader well and should not be
touched. The time remaining display indicates the results are not available and incubation
is in progress.

6 7
1
Do not remove 3M™ Attest™ Biological Indicators until the (+) or (-) symbol on
the LCD panel indicates the test is complete. Once the result is determined, it is
continuously displayed on the LCD panel until 10 seconds after the BI has been removed
from the incubation well.
Note: After the first minute of incubation, if 3M™ Attest™ Biological Indicators are
inadvertently removed before the incubation period is complete, an alarm will sound
and a C2 Caution code will also be displayed on the LCD panel under that well. You must
return the 3M™ Attest™ Biological Indicator to the reader well within 10 seconds
or the results will be lost.
WARNING: Do not remove or change placement of 3M™ Attest™ Biological
Indicator once it is placed into a well. Doing so may result in loss of results and the
biological indicator test may beinvalid.
INSTRUCTIONS FOR USE Positive Control
Positive Control
Activate and incubate at least one nonprocessed 3M™ Attest™ Biological Indicator
to use as the positive control each day that a processed 3M™ Attest™ Biological
Indicator is incubated and read by the unit. Write a C(for control) and the date on
the label. The positive control can be placed in any incubation well. A positive control
should be run for each catalog number, and have the same lot number as the processed
3M™ Attest™Biological Indicator.
It is good practice to use a positive control each day a processed 3M™ Attest™
BiologicalIndicator is incubated. This helps confirm:
• correct incubation temperatures are met (heater block reading of 60ºC +/2ºC).
• viability of spores has not been altered due to improper storage temperature, humidity,
or proximity to chemicals
• capability of media to promote rapid growth, and
• proper functioning of 3M™ Attest™ Autoreader 490 components.
INSTRUCTIONS FOR USE Interpretation of Results
Fluorescent Results
After placing a 3M™ Attest™ Biological Indicator into an incubation well, the 3M™
Attest™ Autoreader 490 LCD panel will display ***, **, * and then the remaining minutes
of incubation directly below the incubating BI to indicate that incubation/reading is in
progress. The 3M™ Attest™ Biological Indicator should not be removed or touched when
the display indicates remaining incubation time.
A (+) will display on the LCD panel directly under the BI and an alarm will sound (if the
sound feature is enabled), as soon as a positive 3M™ Attest™ Biological Indicator result
is detected.
At the end of the specified incubation time, if a negative 3M™ Attest™ Biological
Indicator result is detected, a () will display on the LCD panel directly under the incubating
BI. The final negative fluorescent result () for a processed 3M™ Attest™ Biological
Indicator after 24 minutes of incubation indicates an acceptable sterilization process.
After either the + or symbol is displayed, and the result is recorded, the 3M™ Attest™
Biological Indicator can be removed from the well. The result (+ or ) is displayed on the
LCD panel until the BI is removed from the incubation well. The result clears ten seconds
after the BI has been removed. Do not reincubate BIs as the result will not be valid.
The positive (unprocessed) control 3M™ Attest™ Biological Indicator must provide a
positive fluorescent result (+). Processed 3M™ Attest™ Biological Indicator results
are not valid until the positive control reads fluorescent positive (+). With a processed
3M™ Attest™ Biological Indicator, a positive (+) result indicates a sterilization
processfailure.
Act on any positive test results immediately. Determine the cause of the positive
3M™ Attest™ Biological Indicator and retest the sterilizer following current facility
policies and procedures.
Optional Visual pH Color Change Result - 3MAttestSuper Rapid
Readout Biological Indicators 1491 and 1492V
3M™ Attest™ Super Rapid Readout Biological Indicators may be further incubated for
a visual pH color change. This optional further incubation is not intended for routine use
and may be used for special studies. Due to the high sensitivity of the fluorescent result,
there is no advantage to incubating the 3M™ Attest™ Super Rapid Readout Biological
Indicators 1491 and 1492V beyond 24 minutes. After activation and during incubation,
the white Nonwoven Material in the biological indicator will absorb bromocresol purple
indicator, the pHsensitive indicator dye in the growth media, and appear blue. In the
case of a positive control BI, a yellow color change of the growth media and/or Nonwoven
Material will appear within 24 hours for 1491 BIs and within 48 hours for 1492V BIs.
Any observation of a yellow color within the vial indicates a positive result. In the case of
a processed BI, a media and/or Nonwoven material color change from purple to yellow
indicates a steam sterilization process failure. A negative pH color change result, i.e.
media and Nonwoven Material remain purple/blue, can be assessed at 24 hours for
1491BIs and at 48 hours for 1492V BIs.
Optional Visual pH Color Change Result - 3MAttestRapid
Readout Biological Indicator 1295
3M™ Attest™ Rapid Readout Biological Indicators 1295 for Vaporized Hydrogen Peroxide
Sterilization may be further incubated for a visual pH color change. Due to the high
sensitivity of the fluorescent result, there is no advantage to continuing to incubate 3M™
Attest™ Rapid Readout Biological Indicators 1295 after the fluorescent result has been
determined and documented. This optional further incubation is not intended for routine
use but may be used for special studies.
In the case of a positive control BI, a yellow color change of the growth media will appear
within 24 hours. Any observation of a yellow color within the vial indicates a positive
result. The pH color change result can be recorded and the BI discarded as soon as the
yellow color is observed.
For processed 3M™ Attest™ Rapid Readout Biological Indicators 1295, the use of a
humidified incubator is recommended to avoid media dry out. If a visual pH color change
result is desired, transfer the processed 1295 BI to a humidified incubator after the
fluorescent result has been determined in the 3M™ Attest™ Auto-reader 490. A media
color change from purple to yellow indicates a sterilization process failure.A negative pH
color change result, i.e., media remains purple, can be assessed at 7 days.
INSTRUCTIONS FOR USE Disposal
Instrument contains a lithium battery. Dispose of instrument in accordance with federal,
state, and local requirements.

8 9
1
FEATURES OF THE 3MATTESTAUTOREADER 490
Audible Alarm
An audible alarm will sound, the LCD Display backlight will flash, and a (+) symbol will
be displayed on the LCD panel directly under the incubating BI when a positive result is
detected. An alarm will also sound if a 3M™ Attest™ Biological Indicator is removed from
the 3M™ Attest™ Autoreader 490 well before a final result has been detected. Data
will be lost if the 3M™ Attest™ Biological Indicator is not replaced in the incubation well
within 10 seconds.
Alarm On/Off
The audible alarm for positive results can be turned off for a particular 3M™ Attest™
Biological Indicator. This is accomplished by pressing the Buzzer Alarm OFFbutton
after the alarm has sounded. This will turn off the alarm for that biological indicator only.
Any positive 3M™ Attest™ Biological Indicator results subsequently detected in other
wells will result in the alarm sounding. Removing the 3M™ Attest™ Biological Indicator
will reactivate the alarm for that well. Pressing the Buzzer Alarm OFFbutton will not
silence the alarm for error or caution code alarm warnings. To disable the alarm, see
configurationsection.
Stackable
To save counter space, 3M™ Attest™ Autoreader 490s may be stacked two high.
Information Button
When the Information button is pressed once the 3M™ Attest™ Autoreader 490 displays
two lines of text in the LCD display.
The top row indicates (left to right):
• 3M™ Attest™ Autoreader 490 serial number
• 3M™ Attest™ Autoreader 490 embedded software version (V X.Y.Z)
• Current date and time.
The bottom row indicates:
• The BI type configuration for wells 110 (i.e., Attest)
Press the information button twice and the 3M™ Attest™ Autoreader 490 LCD
panel will display:
• Top row: Dynamic IP address (if unit is connected to an Ethernet jack)
• Bottom row: Date (YYYYMMDD) and time that power was supplied to the 3M™
Attest™ Autoreader 490.
Press the information button three times and the 3M™ Attest™ Autoreader 490 LCD
display will present the unique Media Access Control (MAC) Address for the device.
Temperature Monitoring
The 3M™ Attest™ Autoreader 490 contains an internal temperature selfdiagnostic
program. Should the temperature fall outside the specified range (60ºC +/2ºC) an error
code will be displayed. The heater block temperature may be checked by depressing the
Temperature Button once.
Easy Configuration
The 3M™ Attest™ Autoreader 490 may be configured using either the buttons located
on the front of the unit or using the optional 3M™ Attest™ Autoreader 490 Web App.
Optional 3MAttestAutoreader490 Web App
If desired, the 3M™ Attest™ Autoreader 490 may be connected to a PC via an Ethernet
connection for additional capabilities:
• Remotely view current status of BIs in the 3M™ Attest™ Autoreader 490 via PC.
For full functionality, the use of an HTML5 compliant browser with JavaScript
enabled is required
• Remotely view and configure 3M™ Attest™ Autoreader 490 settings
• View the last 100 BI records
• Print out documentation using a local or networked printer and/or save documentation
as an electronic file.*
* Refer to your facilitys record keeping policy and procedure.
CARE AND CLEANING
CAUTION: Always unplug the 3M™ Attest™ Autoreader 490 and allow to cool
before cleaning.
Do not spill liquid onto or into the instrument. Do not immerse the unit in liquid.
Clean external surfaces using only the instructions provided by the manufacturer.
Do not open the instrument housing there are no user serviceable parts.
The instrument must be returned to the manufacturer for repair.
Clean the exterior surface of the 3M™ Attest™ Autoreader 490 by wiping it with a damp
cloth moistened with a mild detergent. Do not attempt to clean interior components. If
additional cleaning of the wells is required, call for repair or replacement.
Note: The 3M™ Attest™ Autoreader 490 does not contain any user serviceable parts or
require any routine maintenance or calibration. Any malfunctions will be indicated by an
error code. Refer to the Troubleshooting Guide section for error code explanations.

8 9
1
TROUBLESHOOTING GUIDE
In the event of an error code, unplug the 3M™ Attest™ Autoreader 490 and plug it back in to initiate the selfdiagnostic test. If error code reoccurs, call for repair or replacement.
The LCD Display panel will indicate detected malfunctions by displaying a caution or error code under affected BI wells. The audible alarm, if active, sounds and the LCD Display
backlight flashes to alert the user to all caution (except C1) and error codes. The alarm and backlight can be silenced and extinguished, respectively, by pressing the Buzzer Alarm OFF
button . Note that disabling the audible alarm does not disable the flashing LCD Display backlight. Refer to the following:
Code Problem Solution
C1 Unit Warmup Caution
Incubation block not at temperature. Allow a 30 minute warmup after 3M™ Attest™ Autoreader 490 is plugged in. (Note: 3M™ Attest™ BI readings are disabled until incubator block reaches 60ºC +/2ºC).
C2 BI Removed from Well Caution
3M™ Attest™ BI has been removed before incubation
iscomplete.
Replace 3M™ Attest™ BI in proper well within 10 seconds to prevent loss of collected data.
C4 Ambient Light Caution Move 3M™ Attest™ Autoreader490 to reduced ambient lightconditions.
E1 Temperature Control Error
This error will occur if system is no longer able to control the
temperature of the incubator block (60ºC +/2ºC).
Unplug the unit and plug it back in to clear error.
If error doesnt clear, call for repair or replacement.
E3 Memory Error
3M™ Attest™ Autoreader490 unable to save/read data
frommemory.
Unplug the unit and plug it back in to clear error.
If error doesnt clear, call for repair or replacement.
E7 Communications Error
This error will occur if internal communications havefailed. Unplug the unit and plug it back in to clear error. If error doesnt clear, call for repair or replacement.
E8 LED Error
This error occurs for a specific well if the system detects that a
UV LED or Optics Detection circuit is notworking.
Unplug the unit and plug it back in to clear error.
If error doesnt clear, call for repair or replacement. Do not use affected incubation well.
E11 BI Inserted Incorrectly Error
Incubation time remaining will not display and fluorescent
readings are nottaken.
Remove BI and inspect to verify cap is fully closed, glass ampoule is crushed, and growth media is in
growth chamber. If necessary, repeat BI activation steps and return BI to incubation well, ensuring it is
seated properly.
E12 Power Failure Error
Power to Autoreader was off for too long to provide valid result
for affected BI(s).
Ensure power supply connection to Autoreader is secure. Retest sterilizer with a new BI.
E14 Ambient Light Error (occupied well)
Fluorescent result will notdisplay. Rely on optional visual pH color change result for BI in affected well. Move 3M™ Attest™ Autoreader
490 to reduced ambient light conditions to avoid future occurrences. To clear E14 error code, after
visual pH color change result has been documented and BI has been removed, unplug the 3M™
Attest™ Auto-reader 490 and plug it back in.
Other Unwanted alarm sounds when positive BI detected. Disable alarm, refer to 3MAttestAutoreader 490 Configuration section for instructions.
Other Alarm Sounds
3M™ Attest™ Biological Indicator has been removed from
incubation/reader well prematurely.
Replace 3M™ Attest™ Biological Indicator in incubation/reader well within 10 seconds to prevent
loss of data collected.
Other LCD Panel does not display properly. Cycle power. If problem persists call for repair or replacement.
Other Negative Control BI
BI not properly activated. Media did not contact spore carrier.
Control BI was sterilized.
Ensure BI cap is closed and glass ampoule is crushed.
Flick BI until media flows to spore chamber at bottom of BI.
Use a nonsterilized BI as a control.
Check process indicator on BI cap.
Other Unexplained Positive BI
Adhesive residue on vial.
Test vial is absorbing fluorescent residue from a chemical
indicator or tape.
Do not place tape or labels on vial.
Place the vial so it does not come in direct contact with chemical indicators or tape.
Other 3M™ Attest™ Autoreader 490 will not enter Configuration
mode. Pushbutton Configuration may be disabled on 3M™
Attest™ Autoreader 490 Web App.
On 3M™ Attest™ Autoreader 490 Web App Configuration Page, go to Pushbutton Configuration and
select the Enable radio button. Save changes to begin using new setting.
Other 3M™ Attest™ Autoreader 490 LCD display flashes and
asterisks (***) and/or E11 and/or C4 codes appear on display
below unoccupied incubation wells.
Move 3M™ Attest™ Autoreader 490 away from sunlight or sources of incandescent light.

10 11
1
OPTIONAL 3MATTESTAUTOREADER 490 WEB APP
Web Browser Requirements
The Web Interface is designed to be viewed using an HTML5 compliant browser with
JavaScript enabled (e.g., Google Chrome).
Ethernet Connection:
Connection switch/router with autonegotiation capability to identify the Autoreader as
operating at 10 Mbps and halfduplex, or able to be manually configured as 10 Mbps and
halfduplex.
Connecting to the 3MAttestAutoreader490 Web App
To use the 3M™ Attest™ Autoreader 490 Web App, the 3M™ Attest™ 490 Autoreader
must be connected to the facility network using the supplied Ethernet cable. Consult
with your facility IT personnel for assistance with network connectivity. Each 3M™
Attest™ Autoreader 490 has a unique MAC Address which is presented on the units
LCD Display when the user depresses the Info button three times. The MAC Address is
also provided at the bottom of the 3M™ Attest™ Autoreader 490 Web App Configuration
page. Communication occurs using the http protocol over Port 80. Users may connect to
the 3M™ Attest™ Autoreader 490 Web App using a URL based upon either the 3M™
Attest™ Autoreader 490 serial number or the IP Address. To view the 3M™ Attest™
Autoreader 490 Web App from a computer on a subnet different from that to which the
3M™ Attest™ Autoreader 490 is connected, use the IP Address URL.
Note: To access the 3M™ Attest™ Autoreader 490 Web App within a secure network,
the PC must have access to the secure network; options include connecting via a network
Ethernet cable, signing in to the facilitys wireless network, or connecting via a virtual
private network (VPN).
A. Serial number URL
1. Connect the supplied Ethernet cable from the 3M™ Attest™ Autoreader 490 to an
Ethernet jack.
2. Depress the button once to display the 3M™ Attest™ Autoreader 490 6digit
serial number on the left side of the top row of text on the LCD display.
3. Enter http://attestxxxxxx where xxxxxx represents the units serial number (e.g. http://
attest101127) from Step 2 in PC web browser address bar and press Enter.
4. 3M™ Attest™ Autoreader 490 Web App Status page will display on PC. For future
ease of use, add this page to your web browser Favorites.
B. IP Address URL
The 3M™ Attest™ Autoreader 490 Web App may also be accessed using either a
dynamic or static IP address.
1. Dynamic IP Address
A dynamic IP address is assigned when the Ethernet cable is connected from the 3M™
Attest™ Autoreader 490 to a live Ethernet jack.
Press and release the button twice to display the 3M™ Attest™ Autoreader 490s
dynamic IP address. Enter http://IPaddress (e.g. http://xxx.xxx.xxx.xxx) in the PCs web
browser address bar and press Enter. The 3M™ Attest™ Autoreader 490 Web App Status
page will display.
2. Static IP address
Some facility IT personnel may require devices connecting to the network to use a Static
IP address. If this is the case at your facility, contact the IT department to request a Static
IP address.
Record the static IP address provided by your facility IT department here: ___________.
You may wish to label the Ethernet jack with the assigned address. Enter http://
StaticIPaddress (e.g. http://xxx.xxx.xxx.xxx) in the PCs web browser address bar and
press Enter. The 3M™ Attest™ Autoreader 490 Web App Status page will display.
About the 3MAttestAutoreader490 Web App Pages
The 3M™ Attest™ Autoreader 490 Web App includes four pages:
a) Status page
Status
History
3M™ Attest™ Auto-reader
Copyright © 2017 3M, Inc.
ATTEST ATTEST
1 2 3 4 5
+– 24
67 8 910
+–
SN 100157
2017-09-16 08:31
1. Userentered nickname (optional)
2. Well Configuration
3. Well Number
4. BI Result/Remaining Incubation Time
5. Date and Time
6. 3M™ Attest™ Autoreader490 Serial Number
The Status page provides a realtime representation of the 3M™ Attest™ Autoreader
490 configuration and LCD panel display. The current date and time and 3M™ Attest™
Autoreader 490 serial number are also indicated on this page. From this page, the user
may navigate to:
• the History page by clicking on History on the left of the screen.
• the Results Detail page by clicking on the well number of an occupied well (i.e. a well
that currently has an incubating BI). Clicking on an unoccupied well takes the user to
the History page.
b) Results Detail page
Copyright © 2017 3M, Inc.
3M™ Attest™ Auto-reader Results Detail
Status
History
Print
Result
Start Time
Well#
Serial Number
BI Type
Control?
BI Lot#
Sterilizer#
Load#
Implant?
Technician
Sterilizer Model
Cycle Type/Cycle
Parameters
Chemical
Integrator?/Indicator?
Notes
−
2012-09-16 07:04:41
#2
100157
This page provides an individual record for an incubated BI. For complete documentation,
the user may print a hard copy and/or save the record as an electronic file.
Note 1: The top four rows (highlighted in green) are populated automatically by the
3M™ Attest™ Autoreader 490 and cannot be changed by the user. The remaining rows
are completed by the user to satisfy the documentation requirements of their facility.
Note 2: The Results Detail page refreshes less frequently that the Status page. Until the BI
result is available, the Results Detail page displays remaining minutes of incubation in the
results field.

10 11
1
c) History page
Status
History Well#
#3
#9
#2
#1
#10
#9
#10
Start Time
2017-09-16 08:30:38
2017-09-16 07:19:47
2017-09-16 07:08:18
2017-09-16 07:07:53
2017-09-16 07:05:11
2017-09-16 06:47:08
2017-09-16 06:40:54
Result
...
–
–
+
+
–
+
3M™ Attest™ Auto-reader
ID
1
2
3
4
5
6
7
#2
#1
#10
#9
#10
2017-09-16 06:39:49
2017-09-16 06:39:05
2017-09-16 07:32:00
2017-09-16 07:23:37
2017-09-16 07:20:24
–
+
–
–
+
8
9
10
11
12
The 3M™ Attest™ Autoreader 490 stores results for the last 100 incubated BIs on
embedded software. The 3M™ Attest™ Autoreader 490 Web App History page provides
a summary of these last 100 results.
d) Configuration page
This page allows the configuration of the 3M™ Attest™ Autoreader 490s network and
language settings from the 3M™ Attest™ Autoreader 490 Web App.
Documenting BI Results using the 3MAttestAutoreader490
Web App
Status
History
3M™ Attest™ Auto-reader
Copyright © 2017 3M, Inc.
ATTEST ATTEST
1 2 3 4 5
+– 24
67 8 910
+–
SN 100157
2017-09-16 08:31
1. Open the Results Detail page
After inserting a new 3M™ Attest™ Super Rapid Readout BI in the 3M™ Attest™
Autoreader 490, it is a best practice to open the 3M™ Attest™ Autoreader 490 Web
App Results Detail page for that BI by clicking on the appropriate well number from the
Status page.
2. Enter the specific information for that BI (e.g. BI Type, BI Lot #, sterilizer #, load #,
technician name, etc.) in the provided editable fields. Use the Notes section to capture
information not prescribed in the provided row titles, for example, you may wish to use
this field to record load items.
Copyright © 2017 3M, Inc.
3M™ Attest™ Auto-reader Results Detail
Status
History
Print
Result
Start Time
Well#
Serial Number
BI Type 1492V
Control? No
BI Lot# 2019-11NE
Sterilizer# 2
Load# 3
Implant? Yes
Technician sdf
Sterilizer Model 9600
Cycle Type/Cycle
Parameters 270F, 4 min pre-vac
Chemical
Integrator?/Indicator? Accept
Notes
−
2017-09-16 07:04:41
#2
100157
3. The entered information is saved automatically to temporary web browser storage on
the users PC every 60 seconds, or by leaving the Results Details page.
4. Print/File. Once the BI result is available, print or file the Results Detail page to create
a record. The page may be physically printed and/or saved as an electronic file.
Important! Data is only stored for the last 100 incubatedBIs.
Embedded software in the 3M™ Attest™ Autoreader 490 saves the data from the
autopopulated (i.e. green highlighted) fields. The application relies on the temporary
storage in the users web browser to save the data in the userpopulated fields. In both
cases, data is only stored for the last 100 records i.e., the 101st incubated BI will erase
the information saved for the 1st incubated BI.To retain the Results Detail information for
future reference it is best practice to print the page as soon as the BI result is available.

12 13
1
Using the BI History Page
To view or print an individual record, click on the ID number of the desired record to
navigate to the Results Detail page for that BI.
Status
History Well#
#3
#9
#2
#1
#10
#9
#10
Start Time
2017-09-16 08:30:38
2017-09-16 07:19:47
2017-09-16 07:08:18
2017-09-16 07:07:53
2017-09-16 07:05:11
2017-09-16 06:47:08
2017-09-16 06:40:54
Result
...
–
–
+
+
–
+
3M™ Attest™ Auto-reader
ID
1
2
3
4
5
6
7
#2
#1
#10
#9
#10
2017-09-16 06:39:49
2017-09-16 06:39:05
2017-09-16 07:32:00
2017-09-16 07:23:37
2017-09-16 07:20:24
–
+
–
–
+
8
9
10
11
12
Note 1: Column 1, IDis the assigned ID number with ID 1 representing the newest
record and ID 100 representing the oldest record. To help find a particular record, all
columns are sortable, simply click once on the column heading to sort in ascending order
and click a second time to reverse the order.
Note 2: The History page refreshes less frequently than the Status page. Until the BI result
is available, the History page displays  in the Results column indicating incubation is
still inprocess.
Using the Configuration Page
Certain setup and configuration options are available on the password protected 3M™ Attest™ Autoreader 490 Web App Configuration page. Ideally, the 3M™ Attest™ Autoreader
490 should be empty (i.e. have no incubating BIs) when it is reconfigured. Navigate to the Configuration page by entering either:
1) http://attestxxxxxx/protect/config.htm (where xxxxxx is the 6digit unit serial number), or
2) http:// xxx.xxx.xxx.xxx/protect/config.htm (where xxx.xxx.xxx.xxx is the IP address) in the web browser address bar and pressing Go or enter. A security screen will appear. Fill in the
editable fields as follows:
User name: manager
Password: Attest
and pressOK.

12 13
1
Click on the desired radio buttons to changesettings.
Language Selected setting indicates language in which text on 3M™ Attest™ Autoreader 490 Web App pages will appear on the computer screen.
Pushbutton
Configuration Selecting Enable allows the user to configure the 3M™ Attest™ Autoreader 490 from the buttons on the unit itself.
Selecting Disable prevents the user from being able to configure the 3M™ Attest™ Autoreader 490 from the buttons on the unit itself.
Buzzer
Configuration Selecting Enable enables the audible alarm. The speaker icon will appear on the LCD panel.
Selecting Disable disables the audible alarm. The speaker icon will disappear from the 3M™ Attest™ Autoreader 490 LCD panel.
Attest Auto-reader
490 Nickname Naming the 3M™ Attest™ Autoreader 490 may be useful in facilities with multiple units. The assigned nickname will appear on the 3M™ Attest™
Autoreader 490 Web App Status page.
Enable DHCP Default: setting is checked indicating the Web App will use the Dynamic IP address automatically assigned when the 3M™ Attest™ Autoreader 490 is
connected to the network. Remove the check if you wish to use a Static IP address assigned by your facility IT.
IP Address Enter Static IP address assigned by IT
Gateway Enter Gateway
Subnet Mask Enter Subnet Mask
Primary DNS Enter Primary DNS
Secondary DNS Enter Secondary DNS.
MAC Address XX:XX:XX:XX:XX:XX (Unique MAC address assigned to device. This value cannot be changed.)
Use Computer Time Check this box if you wish to set the clock onboard the 3M™ Attest™ Autoreader 490 using the computers clock.
Attest Auto-reader
490 Time Alternatively, leave the Use Computer Time box unchecked and complete the date and time boxes to set the 3M™ Attest™ Autoreader 490 clock.
After changing desired settings, click on Save Config button to save your changes. A new screen confirming the settings were successfully changed appears.

14
1
TROUBLESHOOTING THE 3MATTESTAUTOREADER 490 WEB APP
Issue Problem Solution
Unable to access 3M™ Attest™
Auto-reader 490 Web App on
networked computer
Web Pages have been disabled. Enable Web Pages using push buttons on unit (see 3M™ Attest™ Auto-reader 490
Configurationsection)
Unable to access 3M™ Attest™
Autoreader 490 Web App on
networked computer
3M™ Attest™ Autoreader 490 and/or
computer not connected to network.
Autonegotiation issue between the 3M™
Attest™ Autoreader 490 and facility
network switch.
Ensure 3M™ Attest™ Autoreader 490 Ethernet cable is connected to a live jack and that
connection to 3M™ Attest™ Autoreader 490 is secure.
Ensure computer is connected to network.
If failure is with connection attempted using 3M™ Attest™ Autoreader 490 serial number URL,
try connecting using IP address URL.
Switch port setting must be set at 10 Mbps and halfduplex.
Contact facility IT or 3M if problem persists.
Data entered by user on Results
Detail page disappears User entered data is written to temporary web browser storage. Browser may have
cleared data.
Set web browser to accept (allow or enable) cookies.
As a best practice, print or save Results Detail record as soon as BI result is final.
BI result displayed on 3M™
Attest™ Autoreader 490 LCD
panel does not appear on Results
Detailpage
The Results Detail page has not
refreshed yet. Use your browser to refresh the Results Detail page, or wait up to 60 seconds for the page to automatically refresh.
Results Detail page will not open
from the Status page The Results Detail page only opens from the Status page for occupied 3M™
Attest™ Autoreader 490 wells.
Do not attempt to navigate to the Results Detail page from unoccupied wells on the Status page.
REPAIR AND REPLACEMENT
In the U.S.A., 3M™ Attest™ Autoreader490s must be repaired at:
3M Health Care Service Center
Suite 200, Bldg. 502
3350 Granada Avenue North
Oakdale, MN 55128
18002926298
Fax: 18007708016
Outside of the U.S.A., contact your local 3M Subsidiary.
If you have questions, call our Customer Service number: 18002926298.

15
2
Autoreader3MAttest490 2
Veuillez lire et comprendre toutes les informations de sécurité avant
dutiliser ce produit.
TABLE DES MATIÈRES
PRÉFACE............................................................................................................................................................................16
UTILISATION PRÉVUE ........................................................................................................................................................ 16
INDICATIONS DUTILISATION ............................................................................................................................................ 16
GARANTIE.......................................................................................................................................................................... 16
SÉCURITÉ ET PRÉCAUTIONS.............................................................................................................................................16
INFORMATIONS DE SANTÉ ET DE SÉCURITÉ.................................................................................................................... 17
EXPLICATION DES SYMBOLES APPOSÉS SUR LÉTIQUETTE DU PRODUIT ET DELEMBALLAGE................................... 17
CARACTÉRISTIQUES.......................................................................................................................................................... 18
MODE DEMPLOI................................................................................................................................................................ 18
CARACTÉRISTIQUES DE LAUTOREADER 3MATTEST490..................................................................................... 21
ENTRETIEN ET NETTOYAGE............................................................................................................................................... 22
GUIDE DE DÉPANNAGE...................................................................................................................................................... 22
APPLICATION WEB DE LAUTOREADER 3MATTEST490EN OPTION..................................................................... 23
DÉPANNAGE DE LAPPLICATION WEB DE LAUTOREADER 3MATTEST490.......................................................... 26
RÉPARATION ET REMPLACEMENT....................................................................................................................................26

16 17
2
PRÉFACE
Historique des révisions du manuel
Date Révision Motif de la révision
SEP
2010 A Édition initiale
FEV
2012 BPour inclure lincubation des indicateurs biologiques à lecture
super rapide 3M™ Attest™ 1492V dans lAutoreader
3M™ Attest™ 490et ajouter des déclarations permettant
de clarifier l'accès à l'application Web de lAutoreader
3M™ Attest™ 490sur un réseausécurisé.
JUIN
2013 C Pour réviser: la déclaration de garantie; et les symboles DEEE et CTick et le texte explicatif associé.
SEP
2017 DCe manuel est applicable aux Auto-readers 3M™ Attest™ 490 dotés de la version logicielle 4.0.0 ou ultérieure et d’un
autocollant de configuration de puits d'incubation noir.
Révisé pour: refléter la diminution du temps requis pour le
résultat fluorescent final; ajouter diverses révisions en matière
de conformité; et ajouter des lignes pour les adresses MAC
et DNS secondaire à la page de configuration de l'application
Web490.
FEV
2018 E Manuel révisé pour ajouter: incubation des indicateurs biologiques à lecture rapide Attest™ 1295pour la stérilisation
par vaporisation de peroxyde dhydrogène dans les
Autoreaders 3M™ Attest™ 490dotés de la version logicielle
4.0.0ou ultérieure; et option permettant de désactiver
l'application Web 490.
AOÛT
2018 F Manuel révisé pour ajouter le symbole de date de fabrication et corriger des coquilles.
Exclusions de responsabilité pour le contenu
Exclusion de responsabilité pour les images
Les modèles dimprimés, de graphiques, daffichages et décrans sont proposés à
titre dinformation et dillustration uniquement, et ne doivent pas être utilisés pour
des évaluations cliniques ou de maintenance. Les données illustrées sur les modèles
dimprimés et décrans ne reflètent pas les noms de patients ou les résultats de
testsréels.
Exclusion de responsabilité pour le matériel
Les spécifications pour le matériel de lAutoreader 3M™ Attest™ 490sont susceptibles
dêtre modifiées. Les images, spécifications et composants matériels du système inclus
dans ce manuel peuvent différer du système installé. Toute modification de linstallation
autorisée du système a été vérifiée et est compatible avec les fonctions décrites dans
cedocument.
UTILISATION PRÉVUE
Le système dindicateurs biologiques à lecture super rapide 3M™ Attest™ permet un
contrôle de routine rapide et fiable des processus de stérilisation par vaporisation réalisés
dans les établissements de santé. Ce système comprend des indicateurs biologiques, des
paquetstests et des incubateurs/lecteurs (Autoreader 3M™ Attest™ 490et Autoreader
3M™ Attest™ 490H dotés de la version logicielle 4.0.0 ou ultérieure) avec logiciel intégré.
LAutoreader 3M™ Attest™ 490doté de la version logicielle 4.0.0ou ultérieure est
conçu pour incuber et lire automatiquement les indicateurs biologiques à lecture super
rapide 3M™ Attest™ 1491et 1492V à 60°C pour une lecture finale de fluorescence
négative à 24minutes. LAutoreader 3M™ Attest™ 490est également conçu pour
permettre de poursuivre lincubation dindicateurs biologiques à lecture super rapide
3M™ Attest™, références catalogue 1491et 1492V, pour un résultat final négatif du
changement visuel de couleur du pH respectivement à 24heures et 48heures. Cette
incubation supplémentaire est facultative. Elle nest pas destinée à une utilisation
deroutine.
Les Autoreaders 3M™ Attest™ 490dotés de la version logicielle 4.0.0ou ultérieure
peuvent également être utilisés au sein du système dindicateurs biologiques à lecture
rapide 3M™ Attest™ pour la stérilisation par vaporisation de peroxyde dhydrogène
pour assurer un contrôle de routine rapide et fiable des processus de stérilisation par
vaporisation de peroxyde dhydrogène dans les établissements de santé. Ce système se
compose dun incubateur/de lecteurs (Autoreader 3M™ Attest™ 490doté de la version
logicielle 4.0.0ou ultérieure et Autoreader 3M™ Attest™ 490H) avec logiciel intégré,
et dun indicateur biologique, lindicateur biologique à lecture rapide 3M™ Attest™
1295. LAutoreader 3M™ Attest™ 490doté de la version logicielle 4.0.0ou ultérieure
est conçu pour incuber et lire automatiquement les indicateurs biologiques à lecture
rapide 3M™ Attest™ 1295à 60°C pour une lecture finale de fluorescence négative à
24minutes.
Une lecture de fluorescence positive ou un changement visuel de couleur du pH indique
un échec du processus de stérilisation. Toutefois, en raison de la grande sensibilité du
résultat fluorescent, il nexiste aucun avantage à continuer à incuber des indicateurs
biologiques 3M™ Attest™ traités une fois le résultat fluorescent documenté.
Les autres indicateurs biologiques (IB) ne sont pas compatibles avec ce dispositif, et ne
peuvent pas être utilisés.
INDICATIONS DUTILISATION
LAutoreader 3M™ Attest™ 490est conçu pour incuber et lire automatiquement les
indicateurs biologiques à lecture rapide 3M™ Attest™ 1295et les indicateurs biologiques
à lecture super rapide 3M™ Attest™, références catalogue 1491et 1492V, à 60°C pour
un résultat fluorescent final à 24minutes.
GARANTIE
Aux ÉtatsUnis, lAutoreader 3M™ Attest™ 490bénéficie dune garantie produit limitée
dun an. La garantie, les recours et les limitations pour les ÉtatsUnis sont décrits dans le
formulaire de proposition de prix et sur les pages de prix direct. Pour les autres pays que
les ÉtatsUnis, toute garantie est établie par la filiale avec la responsabilité de lentretien
de lAutoreader 3M™ Attest™ 490.
Numéro de série
Pour faciliter lidentification, chaque Autoreader 3M™ Attest™ 490a un numéro de
série unique imprimé sur une étiquette à larrière de lunité et affiché du côté gauche
de la ligne supérieure sur lécran LCD lorsque lutilisateur appuie sur le bouton .
Veuillez noter votre numéro de série dans ce manuel afin de pouvoir le retrouver par la
suite: ________________________________.
SÉCURITÉ ET PRÉCAUTIONS
LAutoreader 3M™ Attest™ 490et les périphériques et accessoires associés sont
conçus pour assurer un service sûr et fiable conformément aux instructions fournies. Vous
devez lire, comprendre et vous conformer à toutes les informations de sécurité contenues
dans le mode demploi fourni avec lAutoreader 3M™ Attest™ 490, lindicateur
biologique à lecture rapide 3M™ Attest™ 1295et les indicateurs biologiques à lecture
super rapide 3M™ Attest™ 1491et 1492V avant de les utiliser. Utilisez cet équipement
uniquement aux fins décrites dans le Manuel de lutilisateur. Conservez ces instructions
pour référence future.
Cette unité est conçue pour être utilisée uniquement avec le module dalimentation et
le câble Ethernet fournis par 3M. Si ce produit est utilisé dune manière non spécifiée, la
protection assurée par le produit peut être affectée.
Explication des conséquences des mentions davertissement
AVERTISSEMENT: indique une situation dangereuse qui, si elle nest pas évitée,
peut provoquer la mort ou une grave blessure.
ATTENTION: indique une situation dangereuse qui, si elle nest pas évitée, peut
provoquer une blessure légère ou modérée.
Avertissements et mises en garde
Les avertissements et mises en garde suivants doivent être suivis afin déviter des actions
dangereuses susceptibles de provoquer des blessures ou des dommages matériels.
AVERTISSEMENT: pour réduire le risque associé à une tension dangereuse
Utiliser en intérieur uniquement.
Nutilisez pas l'équipement sil ne fonctionne pas correctement ou sil a subi
desdommages.
Utilisez uniquement l'alimentation spécifiée pour ce produit et certifiée pour le pays
dutilisation.

16 17
2
ATTENTION: pour réduire le risque de blessure ou de dommages sur
linstrument
Ne pas renverser de liquide sur ni dans linstrument. Ne pas immerger lunité dans
unliquide.
Toujours débrancher lAutoreader 3M™ Attest™ 490et le laisser refroidir avant
lenettoyage.
Nettoyer les surfaces externes uniquement selon les instructions fournies par
lefabricant.
Ne pas ouvrir le logement de linstrument. Il ny a aucune pièce pouvant être
entretenue par lutilisateur. Linstrument doit être retourné au fabricant afin
dêtreréparé.
Porter des gants et des lunettes de sécurité lors de l'activation de lindicateur
biologique à lecture rapide 3M™ Attest™ 1295.
Laisser refroidir les indicateurs biologiques à lecture super rapide 3M™ Attest™
1491et 1492V pendant la durée recommandée avant de les activer. L'activation ou
la manipulation excessive de lindicateur biologique avant son refroidissement peut
provoquer l'éclatement de lampoule.
Porter des lunettes de sécurité lors de l'activation des indicateurs biologiques à lecture
super rapide 3M™ Attest™ 1491et 1492V.
AVERTISSEMENT: pour réduire le risque associé à des résultats incorrects
Linstrument doit être utilisé par des opérateurs familiarisés avec ce dispositif, ses
fonctions et le Manuel de l'utilisateur.
Ne pas placer linstrument dans un environnement exposé à la lumière du soleil ou à
une forte lumière incandescente.
Ne pas placer linstrument à proximité dun dispositif émettant un champ
électromagnétique puissant.
Ne pas utiliser sur une surface vibrante.
Activer et incuber les indicateurs biologiques à lecture rapide 3M™ Attest™
1295dans lheure suivant la fin du cycle de stérilisation.
Ne pas incuber un indicateur biologique 3M™ Attest™ si, après le traitement et
avant lactivation de lIB, il apparaît que lampoule de milieu est brisée. Soumettre le
stérilisateur à un nouveau test avec un nouvel indicateur biologique.
Laisser refroidir les indicateurs biologiques à lecture super rapide 3M™ Attest™
1491et 1492V pendant la durée recommandée avant de les activer.
Ne pas retirer ni déplacer lindicateur biologique 3M™ Attest™ après l'avoir placé
dans un puits.
Ne pas retirer lindicateur biologique 3M™ Attest™ du puits dincubation avant que le
signe (+) ou () sur l'écran LCD indique que le test est terminé.
ATTENTION: pour réduire le risque associé à des résultats incorrects
Pour éviter toute risque que le flacon dindicateur biologique 3M™ Attest™ absorbe un
résidu fluorescent provenant dun ruban ou dun indicateur chimique, placer le flacon
dindicateur biologique 3M™ Attest™ de sorte quil nentre pas en contact direct avec
le ruban ou les indicateurs chimiques.
INFORMATIONS DE SANTÉ ET DE SÉCURITÉ
Conformité du dispositif en termes de sécurité
LAutoreader 3M™ Attest™ 490est conforme aux normes suivantes, comme indiqué
par le certificat CB Scheme et le rapport de test publié par Underwriters Laboratories (UL):
• CEI 610101(2010) 3ème édition. Règles de sécurité pour appareils électriques de
mesurage, de régulation et de laboratoire Partie 1: Exigences générales
• CEI 610102010:20143ème édition. Règles de sécurité pour appareils électriques de
mesurage, de régulation et de laboratoire Partie 2010: Exigences particulières pour
appareils de laboratoire utilisés pour léchauffement des matières.
LAutoreader 3M™ Attest™ 490est indiqué comme un équipement de laboratoire
et porte le symbole UL avec les indicateurs adjacents «C» et «US» en raison de sa
conformité aux normes UL 610101, CAN/CSA C22.2n° 610101et CAN/CSA C22.2n°
610102010.
LAutoreader 3M™ Attest™ 490est conforme au symbole CE concernant la directive
basse tension (DBT) 2006/95/CE, comme le confirme la Déclaration de conformité.
LAutoreader 3M™ Attest™ 490est conforme à la directive RoHS, Directive 2011/65/
UE du Parlement Européen et du Conseil du 8juin2011sur la restriction de lutilisation de
certaines substances dangereuses dans les équipements électriques et électroniques.
LAutoreader 3M™ Attest™ 490est conforme à la directive DEEE, Directive 2012/19/
UE du Parlement Européen et du Conseil du 04juillet2012sur les déchets déquipements
électriques et électroniques (DEEE).
Conformité CEM
LAutoreader 3M™ Attest™ 490est conforme aux normes CEM suivantes, comme le
confirme le Certificat de conformité produit par 3M:
• CEI 613261:2012/EN 61326:2013 Appareils électriques de mesurage, de régulation
et de laboratoire Exigences de CEM Partie 1: Exigences générales
• Exigences de CEM du symbole CE Directive CEM 2004/108/CE.
LAutoreader 3M™ Attest™ 490est conforme aux exigences de compatibilité
électromagnétique et de sécurité électrique dAustralie et de NouvelleZélande, comme
le confirme la Déclaration de conformité du fabricant associée au RCM (symbole de
conformité réglementaire) australien/néozélandais.
Remarque: cet équipement a été testé et déclaré conforme aux limites pour un appareil
numérique de classe A conformément à la partie 15, souspartie B, des règlements de
la FCC. Ces limites sont conçues pour fournir une protection raisonnable contre toute
interférence nuisible lorsque léquipement est utilisé dans un environnement résidentiel.
Cet équipement produit, utilise et peut émettre de lénergie radio électrique et, sil
nest pas installé et utilisé conformément au manuel dinstructions, peut produire des
interférences nuisibles aux communications radio. Lutilisation de cet appareil dans une
installation résidentielle peut produire des interférences nuisibles, auquel cas lutilisateur
sera tenu dy remédier à ses propres frais.
Cet appareil numérique de classe A respecte toutes les exigences du Règlement sur le
matériel brouilleur du Canada.
EXPLICATION DES SYMBOLES APPOSÉS SUR LÉTIQUETTE
DU PRODUIT ET DELEMBALLAGE
Attention, consulter le mode demploi
Li
Déchets d'équipements électriques et électroniques (DEEE) et directive
européenne relative aux piles et accumulateurs. Ce symbole indique que
le dispositif et la batterie ionlithium qu'il contient doivent être mis au
rebutcorrectement.
Laboratoires habilités (ou UL) à délivrer les certificats de conformité aux
normes américaines etcanadiennes
Symbole de conformité aux normeseuropéennes
Courantdirect
Conforme à toutes les dispositions réglementaires en vigueur de
l'ACMA(RCM)
Fabricant
Date de fabrication
Numéro de référence
Représentant autorisé pour la Communautéeuropéenne
Numéro desérie

18 19
2
CARACTÉRISTIQUES
Caractéristiques de lalimentation
Alimentation Condition d’utilisation Unités
Plage de tension 100à 240 Voltsc.a.
Fréquence 50/60 Hertz
Intensité 0,6 Ampères
Conditions d’utilisation environnementales
Condition environnementale Conditions d’utilisation Unités
Altitude 3000(max) Mètres
Température de service 16à 40 ºC
Température de stockage 10à 50 ºC
Humidité relative de fonctionnement 20à 80(sans condensation) %
Plage de tension 12 Voltsc.c.
Fréquence c.c.
Intensité 1,2 Ampères
Installation/Surtension CatégorieII
Niveau de pollution 2
MODE DEMPLOI Installation initiale
Choix de lemplacement
Placez lAutoreader 3M™ Attest™ 490sur une surface ferme et plane, à l'écart de la
lumière du soleil et de la lumière incandescente. Il est possible dempiler les unités par
deux afin de gagner de la place.
Branchement de lunité
Branchez le cordon d'alimentation à l'alimentation électrique. Branchez l'alimentation à
lAutoreader 3M™ Attest™ 490et branchez le cordon d'alimentation dans une prise
c.a. mise à la terre.
AVERTISSEMENT:
• Ne pas placer linstrument dans un environnement exposé à la lumière du soleil ou
à une forte lumière incandescente.
• Ne pas placer linstrument à proximité dun dispositif émettant un champ
électromagnétique puissant.
• Ne pas utiliser sur une surface vibrante.
Attendre 30minutes avant dutiliser
Laisser chauffer les indicateurs biologiques pendant 30minutes après les avoir
branchés avant de les placer dans les puits dincubation. Le code de mise en garde
C1disparaît une fois la température dincubation appropriée atteinte.
Considérations spéciales
Il est recommandé de laisser l’unité sous tension afin d’éliminer les périodes de
préchauffage. Lunité peut être éteinte lorsquil nest pas prévu de lutiliser pendant une
période prolongée.
AVERTISSEMENT: utilisez uniquement l'alimentation spécifiée pour ce produit
et certifiée pour le pays dutilisation.

18 19
2
MODE DEMPLOI Puits, boutons et interprétation des affichages
1
11
9
4
7
8
2
3
5
10
6
Veuillez vous familiariser avec la façade de lAutoreader 3M™ Attest™ 490:
Puits
1. Puits dincubation/lecteur LAutoreader 3M™ Attest™ 490compte dix puits
dincubation.
Boutons
2. Bouton dinformations / flèche vers le haut
3. Bouton de température / flèche vers le bas
4. Bouton de buzzer éteint / Entrée
Écran dinformations
5. Écran LCD Rétroéclairage.
6. Écran LCD Exemple de message derreur pour le puits n° 6
7. Écran LCD Exemple de durée dincubation restante pour le puits n° 10
8. Écran LCD Exemple de résultat dIB négatif ( ) pour le puits n° 9
9. Écran LCD Exemple de résultat dIB positif ( + ) pour le puits n° 8
10. Écran LCD Indicateur dalarme activée
Indicateur de configuration
11. Létiquette de configuration du puits dincubation est noire.
MODE DEMPLOI Configuration de lAutoreader
3MAttest490
Remarque: idéalement, lAutoreader 3M™ Attest™ 490devrait être vide (aucun IB en
incubation) lors de sa reconfiguration.
Appuyez sur les boutons et à lavant de lAutoreader 3M™ Attest™
490simultanément pendant 3secondes pour entrer en mode de configuration. Dans le
mode de configuration, utilisez les boutons Haut et Bas pour régler lhorloge,
lalarme sonore et lapplication Web de lAutoreader 3M™ Attest™ 490comme vous
le souhaitez. Le bouton sert de touche «Entrée» et déplace le curseur clignotant
jusquau paramètre modifiable suivant.
Réglage de lhorloge de lAutoreader 3MAttest490
La date et lheure de lAutoreader 3M™ Attest™ 490sont affichées au format suivant:
AAAAMMJJ HH:MM, avec lheure affichée au format 24heures. Pour modifier lannée,
si nécessaire, appuyez sur les boutons vers le haut ou vers le bas pour définir lannée
souhaitée, puis appuyez sur pour passer au mois, modifiez si nécessaire et appuyez
sur pour passer au jour. Utilisez le bouton pour passer au réglage des heures
et des minutes.
Réglage de la fonction dalarme sonore
Déplacezvous sur la ligne de texte supérieure en appuyant sur le bouton . Le B
clignotant dans le buzzer indique que ce paramètre peut être modifié. Il est recommandé
de définir «Buzzer Enabled» (Buzzer activé) afin dalerter lopérateur acoustiquement dun
IB positif, mais il est possible de définir «Buzzer Disabled» (Buzzer désactivé) à laide du
bouton Haut ou Bas.
Remarque: si «Buzzer Disabled» (Buzzer désactivé) est défini et si la modification est
acceptée, licône de hautparleur ( ) disparaît de lécran LCD et lalarme est désactivée
pour les indicateurs biologiques 3M™ Attest™ positifs, les codes derreur et les alarmes
de code davertissement.
Remarque: le rétroéclairage de lécran de lAutoreader 3M™ Attest™ 490clignote
dès quune alarme sonore retentit. La désactivation de lalarme sonore nentraîne PAS la
désactivation du clignotement du rétroéclairage de lécran LCD.
Appuyez sur pour sélectionner loption souhaitée et passer au paramètre
modifiablesuivant.
Réglage de la fonction dapplication Web de lAutoreader
3MAttest490en option
Il est recommandé dutiliser le paramètre Web Pages Enabled (Pages Web activées)
par défaut. Toutefois, si vous le souhaitez, vous pouvez désactiver lapplication Web de
lAutoreader 3M™ Attest™ 490en appuyant sur le bouton vers le haut ou vers le bas.
Appuyez sur pour sélectionner loption souhaitée et passer à lécran dacceptation.
Remarque: la désactivation de lapplication Web de lAutoreader 3M™ Attest™
490naffecte pas la connectivité aux logiciels tiers.