Affymetrix PackageInsert Axiom User manual

PackageInsert
For Research Use Only
Not for use in diagnostic procedures.
Product Description
Axiom®Microbiome Array Plate is designed for the detection of microbial
organisms within samples. This array plate, when used with Axiom®2.0 Reagent
Kit (P/N 901758 and 902910) enables researchers to perform small or large-scale
microbiome profiling studies with minimal hands-on processing per plate.
Each plate consists of 24 or 96 microarrays and is offered as a part of a complete
automated solution from Affymetrix that includes instrumentation for sample
preparation, array washing, staining and scanning.
Microbial genomes are amplified using the Axiom 2.0 Reagent Kit and are
present as short randomly generated fragments of genomic DNA (25 to 125
base pair) prior to array hybridization. The reagents for processing Axiom arrays
are also included in this reagent kit.
Axiom Microbiome Array Plates are: For research use only. Not for use in
diagnostic procedures.
Oligonucleotide probes on Axiom array plates are synthesized in situ using
Affymetrix’ photolithographic process.
Instructions for Use
Refer to the following documents for instructions on processing samples using
the Axiom®2.0 Assay.
Related Documents
 Axiom®Microbiome Solution User Guide, P/N 703408
The following documents contain information related to the instruments and
software required to perform the Axiom assay:
 Axiom®2.0 Assay on the Affymetrix®NIMBUS®Target Preparation
Instrument, P/N 703349
 Axiom®2.0 Assay for Affymetrix®NIMBUS® Site Preparation Guide, P/N
703350
 Axiom®2.0 Assay for Affymetrix®NIMBUS®Automated Target Prep Protocol
QRC, P/N 703351
 Axiom®2.0 Assay Manual Workflow User Guide, P/N 702990
 Axiom®2.0 Assay Manual Workflow Site Preparation Guide, P/N 702991
 Axiom®2.0 Assay Manual Target Prep Protocol QRC, P/N 702989
 Axiom®2.0 Assay for 24 Samples Manual Workflow User Guide, P/N 703335
 Axiom®2.0 Assay for 24 Samples Manual Workflow Site Preparation Guide,
P/N 703336
 Axiom®2.0 Assay for 24 Samples Manual Target Preparation Protocol QRC,
P/N 703337
 Axiom®2.0 gDNA Sample Prep Protocol QRC, P/N 702987
 GeneTitan®MC Protocol for Axiom 2.0 Array Plate Processing QRC,
P/N 702988
 GeneTitan®Multi-Channel Instrument User Guide, P/N 08-0306
 GeneTitan®Multi-Channel Instrument Site Preparation Guide, P/N 08-0305
 Affymetrix®GeneChip®Command Console®Software User Guide,
P/N 702569
Library Files
Library files contain information about the array design characteristics, probe use
and content, and scanning and analysis parameters. These files are unique for
each array type. Additional information can be located under the specific array
product on the Affymetrix website at:
www.affymetrix.com/support/technical/libraryfilesmain.affx
Reagents, Instrumentation, and Software Required
1. Axiom®2.0 Reagent Kit
2. Axiom®Microbiome Array Plate
3. Affymetrix®NIMBUS®Target Preparation Instrument
4. GeneTitan®Multi-Channel Instrument
5. Axiom™MiDAS®
For a complete list of reagents and consumables required, please refer to the
Axiom®Microbiome Solution User Guide, P/N 703408.
Precautions
1. AXIOM ARRAY PLATES ARE FOR RESEARCH USE ONLY. NOT FOR USE IN
DIAGNOSTIC PROCEDURES.
2. Avoid microbial contamination, which may cause erroneous results.
3. WARNING: All biological specimens and materials with which they
come into contact should be handled as if capable of transmitting
infection and disposed of with proper precautions in accordance with
federal, state, and local regulations. This includes adherence to the
OSHA Bloodborne Pathogens Standard (29 CFR 1910.1030) for blood-
derived and other samples governed by this act. Never pipet by
mouth. Avoid specimen contact with skin and mucous membranes.
4. CAUTION: Exercise standard precautions when obtaining, handling, and
disposing of potentially carcinogenic reagents.
5. Exercise care to avoid cross-contamination of samples during all steps of this
procedure, as this may lead to erroneous results.
6. Use powder-free gloves whenever possible to minimize introduction of
powder particles into sample or array plates.
7. CAUTION: Use care when handling the scan tray as it has protruding
guiding posts that may be sharp and can stick out of the pouch if not
handled carefully.
Ordering Information
P/N Product Name Description
Arrays
902903 Axiom®Microbiome Array Plate One 24-array plate
902904 Axiom®Microbiome Array Plate One 96-array plate
Supporting Products
902910 Axiom®Microbiome Reagent Kit for
four 24-array plates
Sufficient for four 24-Axiom
Microbiome Array Plates
901606 Axiom®GeneTitan®Consumables Kit1Contains all GeneTitan
consumables required to process
one Axiom array plate
901758 Axiom®2.0 Reagent Kit Sufficient for one 96-Axiom
Microbiome Array Plate
1. One hyb tray, one scan tray, and five stain trays with covers are included for use with each array
plate. These consumables are required for processing Axiom array plates on the GeneTitan Multi-
Channel Instrument.
Patents
Products may be covered by one or more of the following patents: U.S. Patent
Nos. 7,332,273; 7,790,389; 8,114,584; 8,273,304; 8,501,122 and other U.S.
or foreign patents.
Trademarks
Affymetrix®, Axiom®, GeneChip®, Command Console®, CytoScan®, DMET™,
Eureka™, Eureka Genomics®, Eureka Genotyping™, Expression Console™,
GeneAtlas®, GeneChip-compatible™, GeneTitan®, Genotyping Console™,
myDesign™, MyGeneChip™, NetAffx®, OncoScan®, Powered by Affymetrix™,
PrimeView®, and ViewRNA®are trademarks or registered trademarks of
Affymetrix, Inc. Please see www.affymetrix.com/trademarks for a complete list of
Affymetrix trademarks. All other trademarks are the property of their respective
owners.
Axiom®Microbiome Array Plate

Axiom®Microbiome Array Plate P/N 703409 Rev. 2
Storage, Handling and Stability
The Axiom array plate should be stored at 2 to 8°C and must not be frozen.
Refer to the expiration date on the package label. Do not use arrays or reagents
after the expiration date.
When Handling the Axiom Array Plate
Remove the array plate from the pouch with gloved hands. The plate is packaged
with a blue plastic base. Do not remove the array plate from this protective base,
or touch the array plate directly. Keep the array plate in the protective base at
all times, including when placed on the GeneTitan Multi-Channel Instrument.
Figure 1. Array Plate Assembly
Note: Displayed action is for demonstration purposes only.
The array plate is packaged with the blue plastic cover.
Array on glass
substrate mounted
on peg
Array plate Blue plastic
cover
Alignment pins
Array plate and
blue cover assembly
When Handling the 96 Plate Scan Tray
Remove the 96 plate scan tray (scan tray) from the pouch with gloved hands.
The scan tray is packaged with a black protective base and cover. Keep the scan
tray in the protective base with the cover at all times prior to loading into the
GeneTitan Multi-Channel Instrument. Do not touch the bottom of the scan tray
directly. The scan tray has protruding guide posts that may be sharp and can
puncture the pouch if not handled carefully. Take the necessary precautions to
avoid injury.
Figure 2. Scan Tray Assembly
Array plate, scan tray and
black plastic cover assembly
Scan tray Black plastic
cover
Alignment pins
Note: Displayed action is for demonstration purposes only.
All movement of the Axiom®array plate is performed during the
fluidics protocol on the GeneTitan®Instrument.
Contact Information
Affymetrix, Inc.
3420 Central Expressway
Santa Clara, CA 95051 USA
E-mail: [email protected]
Tel: [phone] (1-888-DNA-CHIP)
Fax: [phone]
Affymetrix UK Ltd
Voyager, Mercury Park,
Wycombe Lane, Wooburn Green,
High Wycombe HP10 0HH
United Kingdom
E-mail: [e-mail]
UK and Others Tel: [phone]
France Tel: [phone]
Germany Tel: [phone]
Fax: [phone]
Affymetrix Japan, K. K.
ORIX Hamamatsucho Bldg, 7F
1-24-8 Hamamatsucho, Minato-ku
Tokyo 105-0013, Japan
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
Please visit our web site for international distributor contact information
www.affymetrix.com
Limited License
Affymetrix hereby grants to buyer a non-exclusive, non-transferable, non-
sublicensable license to Affymetrix’ Core Product IP to use the product(s), but only
in accordance with the product labels, inserts, manuals and written instructions
provided by Affymetrix. “Core Product IP” is the intellectual property owned or
controlled by Affymetrix as of the shipment date of a product that covers one or
more features of the product that are applicable in all applications of the product
that are in accordance with the product labels, inserts, manuals and written
instructions provided by Affymetrix. The license granted herein to buyer to the
Core Product IP expressly excludes any use that: (i) is not in accordance with the
product labels, inserts, manuals and written instructions provided by Affymetrix,
(ii) requires a license to intellectual property that covers one or more features
of a product that are only applicable within particular fields of use or specific
applications, (iii) involves reverse engineering, disassembly, or unauthorized
analysis of the product and/or its methods of use, or (iv) involves the re-use of a
consumable product. Buyer understands and agrees that except as expressly set
forth, no right or license to any patent or other intellectual property owned or
controlled by Affymetrix is granted upon purchase of any product, whether by
implication, estoppel or otherwise. In particular, no right or license is conveyed
or implied to use any product provided hereunder in combination with a product
or service not provided, licensed or specifically recommended by Affymetrix
for such use. Furthermore, buyer understands and agrees that buyer is solely
responsible for determining whether buyer possesses all intellectual property
rights that may be necessary for buyer’s specific use of the product, including
any rights from third parties.
Copyright
© 2016 Affymetrix, Inc. All rights reserved.