Agilent Technologies 1260 Infinity User manual

User Manual
Agilent 1260 Infinity
Variable Wavelength
Detector

2 Agilent 1260 Infinity Variable Wavelength Detector User Manual
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Manual Part Number
G1314-90011
Edition
Edition 06/2010
Printed in Germany
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Agilent 1260 Infinity Variable Wavelength Detector User Manual 3
In This Manual…
This manual covers the Agilent 1260 Infinity Variable
Wavelength Detectors
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument
overview and internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements,
physical and performance specifications.
3 Installing the Detector
This chapter provides information on unpacking, checking on
completeness, stack considerations and installation of the
module.
4 Using the Detector
This chapter provides information on how to set up the detector
for an analysis and explains the basic settings.
5 How to optimize the detector
This chapter gives hints on how to select the detector
parameters and the flow cell.
6 Troubleshooting and Diagnostics
This chapter describes the detector’s built in diagnostic signals.
G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+

4 Agilent 1260 Infinity Variable Wavelength Detector User Manual
7 Error Information
This chapter describes the meaning of detector error messages,
and provides information on probable causes and suggested
actions how to recover from error conditions.
8TestFunctions
This chapter describes the detector’s built in test functions.
9 Maintenance and Repair
This chapter provides general information on maintenance and
repair of the detector.
10 Maintenance
This chapter describes the maintenance of the detector.
11 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
12 Identifying Cables
This chapter provides information on cables used with the
Agilent 1260 Infinity modules.
A Appendix
This chapter provides addition information on safety, legal and
web.

Agilent 1260 Infinity Variable Wavelength Detector User Manual 5
Contents
1 Introduction to the Variable Wavelength Detector
Introduction to the Detector 12
Optical System Overview 13
Electrical Connections 17
Serial Number Information 19
Instrument Layout 20
Early Maintenance Feedback (EMF) 21
EMF Counter 21
Using the EMF Counters 21
Interfaces 23
Overview Interfaces 25
Setting the 8-bit Configuration Switch 29
Setting the 8-bit Configuration Switch (On-Board LAN) 29
Setting the 8-bit Configuration Switch (without On-Board
LAN) 31
Communication Settings for RS-232C 32
Special Settings 34
2 Site Requirements and Specifications
Site Requirements 38
Power Consideration 38
Power Cords 39
Bench Space 40
Environment 40
Physical Specifications 42

6 Agilent 1260 Infinity Variable Wavelength Detector User Manual
Performance Specifications 43
Specification Conditions 45
3 Installing the Detector
Unpacking the Detector 48
Delivery Checklist 49
Detector Accessory Kit Contents 49
Optimizing the Stack Configuration 50
One Stack Configuration 50
Two Stack Configuration 53
Installing the Detector 55
Flow Connections to the Detector 58
4 Using the Detector
Setting up an Analysis 62
Before Using the System 62
Requirements and Conditions 64
Optimization of the System 66
Preparing the HPLC System 67
Running the Sample and Verifying the Results 76
Special Settings of the Detector 77
Control Settings 77
Online Spectra 78
Scanning with the VWD 79
Analog Output Settings 81
Special Setpoints 82
Peakwidth Settings 83
Optimizing the Detector 85

Agilent 1260 Infinity Variable Wavelength Detector User Manual 7
5 How to optimize the detector
Optimizing the Detector Performance 88
Match the Flow Cell to the Column 88
Set the Detector Parameters 91
6 Troubleshooting and Diagnostics
Overview of the Detector’s Indicators and Test Functions 94
Status Indicators 95
Power Supply Indicator 95
Module Status Indicator 96
Available Tests vs User Interfaces 97
Agilent LabAdvisor Software 98
7 Error Information
What Are Error Messages 100
General Error Messages 101
Timeout 101
Shut-Down 102
Remote Timeout 102
Synchronization Lost 103
Leak 103
Leak Sensor Open 104
Leak Sensor Short 104
Compensation Sensor Open 105
Compensation Sensor Short 105
Fan Failed 106
Open Cover 106
Detector Error Messages 107
Lamp Current Missing 107
Lamp Voltage Missing 107

8 Agilent 1260 Infinity Variable Wavelength Detector User Manual
Lamp Ignition Failed 108
Heater Current Missing 108
Calibration Failed 109
Holmium Oxide Test Failed 110
Grating/Filter Motor Test Failed 110
Wavelength Check Failed 111
Filter Check Failed 111
8TestFunctions
Available Tests versus Interfaces 114
Intensity Test 115
Checking the Photocurrent 117
Wavelength Verification/Calibration 118
ASTM Drift and Noise Test 122
Quick Noise Test 123
Dark Current Test 124
Holmium Oxide Test 126
D/A Converter (DAC)Test 128
Grating and Filter Motor Test 131
Test Chromatog r a m 133
Procedure Using the Agilent LabAdvisor 133
9 Maintenance and Repair
Introduction to Maintenance 136
Warnings and Cautions 137

Agilent 1260 Infinity Variable Wavelength Detector User Manual 9
10 Maintenance
Overview of Maintenance 140
Cleaning the Module 141
Exchanging a Lamp 142
Exchanging a Flow Cell 145
Repairing the Flow Cells 148
Using the Cuvette Holder 151
Correcting Leaks 154
Replacing Leak Handling System Parts 155
Replacing the Interface Board 156
Replacing the Detector’s Firmware 157
11 Parts and Materials for Maintenance
Overview of Maintenance Parts 160
Standard Flow Cell 161
Micro Flow Cell 163
Semi-micro Flow Cell 164
High Pressure Flow Cell 166
Cuvette Holder 167
Leak Parts 168
Accessory Kit 169

10 Agilent 1260 Infinity Variable Wavelength Detector User Manual
12 Identifying Cables
Cable Overview 172
Analog Cables 174
Remote Cables 176
BCD Cables 179
CAN / LAN Cables 181
RS-232 Cable 182
External Contact Cable 183
A Appendix
General Safety Information 186
Safety Standards 186
Safety Symbols 188
Lithium Batteries Information 189
Radio Interference 190
Sound Emission 191
UV-Radiation 192
Solvent Information 193
Declaration of Conformity for HOX2 Filter 195
Agilent Technologies on Internet 196