Agilent Technologies 1260 Infinity User manual

User Manual
Agilent 1260 Infinity
Variable Wavelength
Detector

2 Agilent 1260 Infinity Variable Wavelength Detector User Manual
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Manual Part Number
G1314-90011
Edition
Edition 06/2010
Printed in Germany
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Agilent 1260 Infinity Variable Wavelength Detector User Manual 3
In This Manual…
This manual covers the Agilent 1260 Infinity Variable
Wavelength Detectors
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument
overview and internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements,
physical and performance specifications.
3 Installing the Detector
This chapter provides information on unpacking, checking on
completeness, stack considerations and installation of the
module.
4 Using the Detector
This chapter provides information on how to set up the detector
for an analysis and explains the basic settings.
5 How to optimize the detector
This chapter gives hints on how to select the detector
parameters and the flow cell.
6 Troubleshooting and Diagnostics
This chapter describes the detector’s built in diagnostic signals.
G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+

4 Agilent 1260 Infinity Variable Wavelength Detector User Manual
7 Error Information
This chapter describes the meaning of detector error messages,
and provides information on probable causes and suggested
actions how to recover from error conditions.
8TestFunctions
This chapter describes the detector’s built in test functions.
9 Maintenance and Repair
This chapter provides general information on maintenance and
repair of the detector.
10 Maintenance
This chapter describes the maintenance of the detector.
11 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
12 Identifying Cables
This chapter provides information on cables used with the
Agilent 1260 Infinity modules.
A Appendix
This chapter provides addition information on safety, legal and
web.

Agilent 1260 Infinity Variable Wavelength Detector User Manual 5
Contents
1 Introduction to the Variable Wavelength Detector
Introduction to the Detector 12
Optical System Overview 13
Electrical Connections 17
Serial Number Information 19
Instrument Layout 20
Early Maintenance Feedback (EMF) 21
EMF Counter 21
Using the EMF Counters 21
Interfaces 23
Overview Interfaces 25
Setting the 8-bit Configuration Switch 29
Setting the 8-bit Configuration Switch (On-Board LAN) 29
Setting the 8-bit Configuration Switch (without On-Board
LAN) 31
Communication Settings for RS-232C 32
Special Settings 34
2 Site Requirements and Specifications
Site Requirements 38
Power Consideration 38
Power Cords 39
Bench Space 40
Environment 40
Physical Specifications 42

6 Agilent 1260 Infinity Variable Wavelength Detector User Manual
Performance Specifications 43
Specification Conditions 45
3 Installing the Detector
Unpacking the Detector 48
Delivery Checklist 49
Detector Accessory Kit Contents 49
Optimizing the Stack Configuration 50
One Stack Configuration 50
Two Stack Configuration 53
Installing the Detector 55
Flow Connections to the Detector 58
4 Using the Detector
Setting up an Analysis 62
Before Using the System 62
Requirements and Conditions 64
Optimization of the System 66
Preparing the HPLC System 67
Running the Sample and Verifying the Results 76
Special Settings of the Detector 77
Control Settings 77
Online Spectra 78
Scanning with the VWD 79
Analog Output Settings 81
Special Setpoints 82
Peakwidth Settings 83
Optimizing the Detector 85

Agilent 1260 Infinity Variable Wavelength Detector User Manual 7
5 How to optimize the detector
Optimizing the Detector Performance 88
Match the Flow Cell to the Column 88
Set the Detector Parameters 91
6 Troubleshooting and Diagnostics
Overview of the Detector’s Indicators and Test Functions 94
Status Indicators 95
Power Supply Indicator 95
Module Status Indicator 96
Available Tests vs User Interfaces 97
Agilent LabAdvisor Software 98
7 Error Information
What Are Error Messages 100
General Error Messages 101
Timeout 101
Shut-Down 102
Remote Timeout 102
Synchronization Lost 103
Leak 103
Leak Sensor Open 104
Leak Sensor Short 104
Compensation Sensor Open 105
Compensation Sensor Short 105
Fan Failed 106
Open Cover 106
Detector Error Messages 107
Lamp Current Missing 107
Lamp Voltage Missing 107

8 Agilent 1260 Infinity Variable Wavelength Detector User Manual
Lamp Ignition Failed 108
Heater Current Missing 108
Calibration Failed 109
Holmium Oxide Test Failed 110
Grating/Filter Motor Test Failed 110
Wavelength Check Failed 111
Filter Check Failed 111
8TestFunctions
Available Tests versus Interfaces 114
Intensity Test 115
Checking the Photocurrent 117
Wavelength Verification/Calibration 118
ASTM Drift and Noise Test 122
Quick Noise Test 123
Dark Current Test 124
Holmium Oxide Test 126
D/A Converter (DAC)Test 128
Grating and Filter Motor Test 131
Test Chromatog r a m 133
Procedure Using the Agilent LabAdvisor 133
9 Maintenance and Repair
Introduction to Maintenance 136
Warnings and Cautions 137

Agilent 1260 Infinity Variable Wavelength Detector User Manual 9
10 Maintenance
Overview of Maintenance 140
Cleaning the Module 141
Exchanging a Lamp 142
Exchanging a Flow Cell 145
Repairing the Flow Cells 148
Using the Cuvette Holder 151
Correcting Leaks 154
Replacing Leak Handling System Parts 155
Replacing the Interface Board 156
Replacing the Detector’s Firmware 157
11 Parts and Materials for Maintenance
Overview of Maintenance Parts 160
Standard Flow Cell 161
Micro Flow Cell 163
Semi-micro Flow Cell 164
High Pressure Flow Cell 166
Cuvette Holder 167
Leak Parts 168
Accessory Kit 169

10 Agilent 1260 Infinity Variable Wavelength Detector User Manual
12 Identifying Cables
Cable Overview 172
Analog Cables 174
Remote Cables 176
BCD Cables 179
CAN / LAN Cables 181
RS-232 Cable 182
External Contact Cable 183
A Appendix
General Safety Information 186
Safety Standards 186
Safety Symbols 188
Lithium Batteries Information 189
Radio Interference 190
Sound Emission 191
UV-Radiation 192
Solvent Information 193
Declaration of Conformity for HOX2 Filter 195
Agilent Technologies on Internet 196

11
Agilent 1260 Infinity Series Variable Wavelength Detector
User Manual
Agilent Technologies
1
Introduction to the Variable Wavelength
Detector
Introduction to the Detector 12
Optical System Overview 13
Flow Cell 14
Electrical Connections 17
Instrument Layout 20
Early Maintenance Feedback (EMF) 21
Interfaces 23
Setting the 8-bit Configuration Switch 29
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

12 Agilent 1260 Infinity Variable Wavelength Detector User Manual
1Introduction to the Variable Wavelength Detector
Introduction to the Detector
The Agilent 1260 Infinity Variable Wavelength Detector is designed for highest
optical performance, GLP compliance and easy maintenance with:
•data rate up to
•13 Hz for standard HPLC with G1314B VWD VL and
•55 Hz for fast-HPLC with G1314C VWD VL+,
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow-cell cartridges (standard 10 mm 14 µL, high pressure 10 mm
14 µL, micro 5 mm 1 µL, semi-micro 6 mm 5 µL) are available and can be
used depending on the application needs,
•easy front access to lamp and flow cell for fast replacement, and
•built-in holmium oxide filter for fast wavelength accuracy verification.
For specifications “Performance Specifications" on page 43.
Two versions of the Agilent 1260 Infinity Variable Wavelength Detector are
available:
G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
standard version
G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+
high data rates for fast HPLC

Introduction to the Variable Wavelength Detector 1
Agilent 1260 Infinity Variable Wavelength Detector User Manual 13
Optical System Overview
The optical system of the detector is shown in Figure 1. Its radiation source is
a deuterium-arc discharge lamp for the ultraviolet (UV) wavelength range from
190 to 600 nm. The light beam from the deuterium lamp passes through a lens,
a filter assembly (in position none, cut-off or holmium oxide), an entrance slit,
a spherical mirror (M1), a grating, a second spherical mirror (M2), a beam
splitter, and finally through a flow cell to the sample diode. The beam through
the flow cell is absorbed depending on the solutions in the cell, in which UV
absorption takes place, and the intensity is converted to an electrical signal by
means of the sample photodiode. Part of the light is directed to the reference
photodiode by the beam splitter to obtain a reference signal for compensation
of intensity fluctuation of the light source. A slit in front of the reference
photodiode cuts out light of the sample bandwidth. Wavelength selection is
made by rotating the grating, which is driven directly by a stepper motor. This
configuration allows fast change of the wavelength. The cutoff filter is moved
into the lightpath above 370 nm to reduce higher order light.
Figure 1 Optical Path of the Variable Wavelength Detector
Deuterium lamp
Filter assembly
Lens
Entrance slit
Mirror M1
Mirror M2
Grating
Reference diode
Sample diode
Flow cell
Beam splitter

14 Agilent 1260 Infinity Variable Wavelength Detector User Manual
1Introduction to the Variable Wavelength Detector
Flow Cell
A variety of flow-cell cartridges can be inserted using the same quick and
simple mounting system.
Figure 2 Cartridge Type Flow Cell
Table 1 Flow Cell Data
STD Semi-micro High Pressure Micro
Maximum pressure 40 (4) 40 (4) 400 (40) 40 (4) bar (MPa)
Path length 10 (conical) 6 (conical) 10 (conical) 5 mm
Volume 14 5 14 1 µL
Inlet i.d. 0.17 0.17 0.17 0.1 mm
Inlet length 750 750 750 555 mm
Outlet i.d. 0.25 0.25 0.25 0.25 mm
Outlet length 120 120 120 120 mm
Materials in contact with
solvent
SST, quartz,
PTFE, PEEK
SST, quartz,
PTFE
SST, quartz,
Kapton
SST, quartz,
PTFE

Introduction to the Variable Wavelength Detector 1
Agilent 1260 Infinity Variable Wavelength Detector User Manual 15
Lamp
The light source for the UV wavelength range is a deuterium lamp. As a result
of plasma discharge in a low pressure deuterium gas, the lamp emits light over
the 190 to 600 nm wavelength range.
Source Lens Assembly
The source lens receives the light from the deuterium lamp and focuses it onto
the entrance slit.
Entrance Slit Assembly
The entrance slit assembly has an exchangeable slit. The standard one has a
1-mm slit. For replacement and calibration purposes to optimize the
alignment, a slit with a hole is needed.
Filter Assembly
The filter assembly is electromechanically actuated. During wavelength
calibrations it moves into the light path.
The filter assembly has two filters installed and is processor-controlled.
A photo sensor determines the correct position.
OPEN nothing in light path
CUTOFF cut off filter in light path at λ>370nm
HOLMIUM holmium oxide filter for wavelength check.
Figure 3 Filter Assembly
Cutoff filter Holmium oxide filter
with cutoff filter

16 Agilent 1260 Infinity Variable Wavelength Detector User Manual
1Introduction to the Variable Wavelength Detector
Mirror Assemblies M1 and M2
The instrument contains two spherical mirrors (M1 and M2). The beam
adjustable is vertically and horizontally. Both mirrors are identical.
Grating Assembly
The grating separates the light beam into all its component wavelengths and
reflects the light onto mirror #2.
Beam Splitter Assembly
The beam splitter splits the light beam. One part goes directly to the sample
diode. The other part of the light beam goes to the reference diode.
Photo Diodes Assemblies
Two photo diode assemblies are installed in the optical unit. The sample diode
assembly is located on the left side of the optical unit. The reference diode
assembly is located in the front of the optical unit.
Photo Diode ADC (analog-to-digital converter)
The photo diode current is directly converted to 20-bit digital data direct
photo current digitalization. The data is transferred to the detector main
board (VWM). The photo diode ADC boards are located close to the photo
diodes.

Introduction to the Variable Wavelength Detector 1
Agilent 1260 Infinity Variable Wavelength Detector User Manual 17
Electrical Connections
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
•One analog output provides signals for integrators or data handling
systems.
•The interface board slot is used for external contacts and BCD bottle
number output or LAN connections.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
•The power input socket accepts a line voltage of 100 – 240 volts AC ± 10%
with a line frequency of 50 or 60 Hz. Maximum power consumption is
220 VA. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations

18 Agilent 1260 Infinity Variable Wavelength Detector User Manual
1Introduction to the Variable Wavelength Detector
Figure 4 Rear View of Detector
Analog Signals
APG Remote
RS-232C
CAN
Power
Configuration switch
Slot for interface
board
Security lever
NOTE The GPIB interface has been removed with the introduction of the 1260 Infinity modules.

Introduction to the Variable Wavelength Detector 1
Agilent 1260 Infinity Variable Wavelength Detector User Manual 19
Serial Number Information
Serial Number Information 1260 Infinity
The serial number information on the instrument labels provide the following
information:
Serial Number Information 1200 Series
The serial number information on the instrument labels provide the following
information:
CCXZZ00000 Format
CC Country of manufacturing (DE Germany)
X Alphabetic character A-Z (used by manufacturing)
ZZ Alpha-numeric code 0-9, A-Z, where each combination
unambiguously denotes a module (there can be more than one
code for the same module)
00000 Serial number
CCYWW00000 Format
CC Country of manufacturing (DE Germany)
YWW year and week of last major manufacturing change, e.g. 820
could be week 20 of 1998 or 2008
00000 Serial number

20 Agilent 1260 Infinity Variable Wavelength Detector User Manual
1Introduction to the Variable Wavelength Detector
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers foam plastic spacers in which the mechanical and
electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
•virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
•the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
•the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
•the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.