Agilent Technologies G1701EA User manual

Agilent Technologies
Agilent G1701EA MSD
Productivity
ChemStation
Familiarization Guide

Notices
© Agilent Technologies, Inc. 2012
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Manual Part Number
G1701-90070
Edition
Second edition, June 2012
Printed in USA
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Santa Clara, CA 95051 USA
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Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 3
In This Guide
This guide contains a step-by-step exercise to help you become
familiar with your Agilent 7890A GC/5975 MSD with G1701EA
MSD Productivity ChemStation software.
To successfully use this book, you need the following:
Before operating your instruments, be sure to read all safety
and regulatory information included with your instruments.
1Start Up the System
Start up your system hardware and software for data
acquisition.
2Tune the MS
Determine whether the instrument is correctly tuned.
3Create a Method for Qualitative Analysis
Create a new qualitative analysis scan method from the system
default method.
4Run the Scan Method
Run the method created in Chapter 3to acquire sample data.
5Qualitative Data Analysis
Use the Enhanced Data Analysis program to analyze the data
generated in Chapter 4.
6Create a SIM Quantitation Method
Create a SIM method from the scan method created in
Chapter 3.
7Run a Sequence
Create and run a sequence using the method created in
Chapter 6.
•GC Inlet: Split/Splitless Inlet with EPC
(default inlet configuration)
•Column: HP-5ms 30 m x 250 µm x 0.25 µm
•Sample: 5975 MSD Sample (P/N 05970-60045) or
(P/N 5074-3025 Japan only)
•MSD Tuning
Calibrator:
PFTBA (perfluorotributylamine)

4 Agilent G1701EA MSD Productivity ChemStation
8Set Up a Quantitation Database
Set up a database with compounds and calibrators to identify
unknown samples.
9Generate a Report
Generate a report automatically after a run or at a later point
from previously acquired data.
10 Recalibrate and Quantitate Unknowns
Modify a sequence for recalibration and then use it to
quantitate an unknown sample.
11 Create a Cool Down Method
Create and store a maintenance method.
12 Shut Down the System
13 Frequently Asked Questions

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 5
Where to Find Information
Hardware
In addition to this document, Agilent provides several learning
products that document how to install, operate, maintain, and
troubleshoot the 7890A GC/5975 MSD. This information can be found
on the Agilent Technologies GC and GC/MS Hardware User
Information and Utilities DVDs that ship with your instrument.
The Agilent Technologies GC and GC/MS Hardware User Information
and Utilities DVDs that ship with your instrument provides an
extensive collection of online help, videos, and books for current
Agilent gas chromatographs, mass selective detectors, ion traps, and
GC samplers. Included are localized versions of the information you
need most, such as:
•Getting Familiar documentation
•Safety and Regulatory guides
•Site Preparation checklists
•Installation information
•Operating guides
•Maintenance information
•Troubleshooting details
Software
For an introduction to, and where to find more information on,
the G1701EA MSD Productivity ChemStation see the Agilent
G1701EA GC/MSD ChemStation Getting Started manual.

6 Agilent G1701EA MSD Productivity ChemStation

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 7
Contents
1 Start Up the System
Start up the Hardware 12
Run the ChemStation Software 14
Select the Tune File 15
Load the Method 16
2 Tune the MS
Introduction 18
Run Autotune 19
Evaluate the Autotune Results 22
Tune History Trends 24
3 Create a Method for Qualitative Analysis
Introduction 26
Edit the Entire Method 27
Check the GC configuration 29
Set the GC readiness state 32
Set the GC oven parameters 33
Set the GC column parameters 35
Set the GC inlet parameters 36
Set the GC injector parameters 38
Set the GC Aux heaters parameters 40
Set the GC signals parameters 40
Edit the GC real time plots to display 42
Edit the MS parameters 42
Save the method 46
General Information for Editing the GC Parameters 47
Open the GC edit parameters window 47
Add a column to ChemStation local inventory 48
Select and configure a column 51
Upload parameters from the 7890A GC 53
Customize the status panel view 53

8 Agilent G1701EA MSD Productivity ChemStation Familiarization Guide
4 Run the Scan Method
Prepare the Sample 56
Load the Method 57
Run the Method 58
Take a Snapshot 61
View the Logbook 62
5 Qualitative Data Analysis
Integrate Peaks 66
Edit the integration events 69
Save the integration events to the method 70
Manually integrate peaks 71
View the integration results in a table 72
Edit the Method to Generate a Report 74
Display Extracted Ion Chromatograms (EIC)s 76
Enable or Disable the Right Mouse Click Context Menu 78
Analyze Data 79
Subtract the baseline noise from the spectra 81
Select target and qualifier ions 82
Search the Spectral Library 83
Generate an automated library search report 84
Print a Window, TIC, Spectrum, or Method 86
Select a printer 86
Select an item to print 87
Save the Data Analysis Method 87
Exit the Data Analysis Program 88
6 Create a SIM Quantitation Method
Introduction 90
Create a SIM Method 91
Simultaneously Acquire Scan and SIM Data (SIM/Scan Mode) 96
SIM/Scan Mode Cycle Frequency 98
7 Run a Sequence
Prepare the Samples 100
Create the Sequence 101

Agilent G1701EA MSD Productivity ChemStation Familiarization Guide 9
Save the Sequence 103
Load the Sequence 104
Run the Sequence 105
Print the Sequence Log 106
8 Set Up a Quantitation Database
Add Compound Entries for the Database 108
Identify compounds 112
Add the Calibration Curve 115
Add calibrator level 1 115
Add calibrator levels 5, 10, 25, and 50 to the calibration curve 117
Save the database 119
View or Edit an Existing Database 120
9 Generate a Report
Generate a Report Automatically After the Run 124
Load the method 124
Edit the method to generate a report 124
Run the method and generate the report 127
Generate a Detailed Report for Previously Acquired Data 129
Load the method 129
Load the data file 129
Generate a detailed quantitation report 129
10 Recalibrate and Quantitate Unknowns
Create a Recalibration Sequence 132
Save the Sequence 134
Run the Sequence 135
11 Create a Cool Down Method
Create the Cool Down Method 138
Use the Cool Down Method 139
12 Shut Down the System
Shut Down the MS 142
Shut Down the GC 143
13 Frequently Asked Questions

10 Agilent G1701EA MSD Productivity ChemStation Familiarization Guide