Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
Quaternary Pump
User Manual

1290 Infinity Quaternary Pump User Manual
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2015
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Manual Part Number
G4204-90000 Rev. F
Edition
02/2015
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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1290 Infinity Quaternary Pump User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1290 Infinity Quaternary Pump (G4204A).
1 Introduction
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your
system and the installation of your Agilent 1290 Infinity Quaternary
Pump.
4UsingthePump
This chapter explains the operational parameters of the Agilent 1290
Infinity Quaternary Pump.
5 How to Optimize the Performance of Your Module
This chapter gives hints on how to optimize the performance or use
additional devices.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 1290 Infinity Quaternary Pump User Manual
In This Guide...
8 Test Functions and Calibrations
This chapter will describe the tests for the module.
9 Maintenance
This chapter describes the maintenance of the Agilent 1290 Infinity
Quaternary Pump.
10 Parts and Materials
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the pump in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the
controller software.
14 Appendix
This chapter provides additional information on safety, legal and web.

1290 Infinity Quaternary Pump User Manual 5
Contents
Contents
1 Introduction 9
Features 10
Overview of the Quaternary Pump 11
Operating Principle 12
Positions of the Multi Purpose Valve 14
System Overview 19
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications 28
3 Installing the Module 31
Unpacking the Module 32
Optimizing the Stack Configuration 34
Installation Information on Leak and Waste Handling 39
Removing the Transport Foam 43
Installing the Pump 44
Flow Connections to the Pump 46
Installation of Seal Wash Option 49
4UsingthePump 51
Leak and Waste Handling 52
Preparing the Pump 53
Algae Growth in HPLC Systems 54
Setting up the Pump with the Instrument Control Interface 55
Priming the Pump 68
Flushing the Filter 70
Solvent Information 72

6 1290 Infinity Quaternary Pump User Manual
Contents
5 How to Optimize the Performance of Your Module 79
Using the Degasser 80
Operational Hints for the Multi Channel Gradient Valve (MCGV) 81
Delay Volume and Extra-Column Volume 82
How to Configure the Optimum Delay Volume 83
How to Achieve Higher Resolution 85
Using Solvent Calibration Tables 88
6 Troubleshooting and Diagnostics 89
Overview of the Module’s Indicators and Test Functions 90
Status indicators 91
Available Tests vs User Interfaces 93
Agilent Lab Advisor Software 94
7 Error Information 95
What Are Error Messages 97
General Error Messages 98
Pump Error Messages 103
8 Test Functions and Calibrations 121
9 Maintenance 123
Introduction to Maintenance 125
Warnings and Cautions 126
Overview of Maintenance 128
Cleaning the Module 129
Installing Fittings and Capillaries 130
Replacing the Pressure Sensor 131
Replacing the Inlet Weaver 134
Replacing the Inlet Valve 136
Replacing the Outlet Valve 137
Removing the Jet Weaver 139
Installing the Jet Weaver 141
Replacing the Seal Wash Pump 143
Replacing the Multi-Channel Gradient Valve (MCGV) 145
Releasing a Stuck Inlet Valve 149
Replacing the Pump Head 152

1290 Infinity Quaternary Pump User Manual 7
Contents
Disassembling the Pump Head 158
Disassembling the Primary Pump Head 160
Disassembling the Secondary Pump Head 164
Replacing the Heat Exchanger 168
Replacing Wash Seal and Gasket 171
Assembling the Pump Head 173
Replacing the Multi Purpose Valve 185
Replacing Parts of the Multi Purpose Valve 188
Replacing the Outlet Filter 189
Replacing Parts of the Inline Filter 191
Installing the Valve Rail Kit 194
Replacing Module Firmware 195
Preparing the Pump Module for Transport 196
10 Parts and Materials 201
Overview of Main Assemblies 202
Flow Connections 204
Seal Wash Option 206
Pump Head Assembly Parts 207
Primary Pump Head Parts 208
Secondary Pump Head Parts 212
Multi Purpose Valve 216
Solvent Cabinet 218
Cover Parts 220
Leak Parts 222
Accessory Kit 224
Others 225
11 Identifying Cables 227
Cable Overview 228
Analog cables 230
Remote Cables 232
BCD Cables 235
CAN/LAN Cable 237
RS-232 Cable Kit 238
Agilent 1200 Module to Printer 239

8 1290 Infinity Quaternary Pump User Manual
Contents
12 Hardware Information 241
Firmware Description 242
Electrical Connections 245
Interfaces 247
Setting the 8-bit Configuration Switch 254
Early Maintenance Feedback 257
Instrument Layout 258
13 LAN Configuration 259
What You Have To Do First 260
TCP/IP Parameter Configuration 261
Configuration Switch 262
Initialization Mode Selection 263
Dynamic Host Configuration Protocol (DHCP) 267
Link Configuration Selection 270
Automatic configuration with Bootp 271
Manual Configuration 281
PC and User Interface Software Setup Setup 287
14 Appendix 289
General Safety Information 290
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002-96-EC) 293
Radio Interference 294
Sound Emission 295
Agilent Technologies on Internet 296

9
1290 Infinity Quaternary Pump User Manual
Agilent Technologies
1
Introduction
Features 10
Overview of the Quaternary Pump 11
Operating Principle 12
Positions of the Multi Purpose Valve 14
System Overview 19
Leak and Waste Handling 19
This chapter gives an introduction to the module, instrument overview and
internal connectors.

10 1290 Infinity Quaternary Pump User Manual
1Introduction
Features
Features
The G4204A Quaternary Pump is designed for highest performance, GLP
compliance and easy maintenance. It includes the following features:
•Optional seal wash for continued high lifetime of pump seals for buffer
applications.
•Optional Jet Weaver for optimum mixing performance with a minimum
of delay volume.
•Automatic purge function for ease of use and unattended preparation of
the system.
•Auto tuning of the delivery cycle for compensation of elasticity and
solvent volume change effects (compressibility, thermal expansion).
•Solvent selection for optimum flow accuracy.
•Fast defill function for improved intake and delivery performance.
•Two pistons in series design for increased reliability.
•High resolution piston movement control for smooth and reliable
motion.
For specifications, see “Performance Specifications” on page 28.

1290 Infinity Quaternary Pump User Manual 11
Introduction 1
Overview of the Quaternary Pump
Overview of the Quaternary Pump
The Agilent 1290 Infinity Quaternary Pump has a built- in 4- channel
vacuum degasser for best flow stability, especially at low flow rates and
maximum detector sensitivity. It uses a multi- channel gradient valve
(MCGV) for formation of quaternary gradients at low pressure. The
low- pressure Inlet Weaver based on patented Agilent microfluidic
technology ensures highest mixing performance and lowest mixing noise.
The pump head offers a high power range with a maximum pressure of
1200 bar and a maximum flow rate of 5 mL/min. The Multi Purpose Valve
can be used for automatic purging, using an optional Jet Weaver
high- performance mixer, automatic back- flushing of the filter or for
diagnostic.
The Agilent 1290 Infinity Quaternary Pump is suitable for a wide range of
columns and HPLC und UHPLC applications starting from typical 250 x
4.6 mm HPLC columns going down to high resolution 50 x 2.1 mm UHPLC
columns and can be used in a flow range between 0.05 – 5 mL/min.
Active seal wash is optionally available for use with concentrated buffer
solutions.

12 1290 Infinity Quaternary Pump User Manual
1Introduction
Operating Principle
Operating Principle
The pump head comprises two pump chambers in series with independent
high- resolution motion control. A pressure sensor in the flow path
monitors the pressure. The pump control uses this signal for minimizing
the pressure ripple in order to achieve highest flow precision. A stable
flow can be delivered even in case of eventual small internal leaks, which
can be compensated automatically. A heat exchanger between two pump
chambers strongly reduces thermal effects due to solvent compression
under very high pressures.
As solvents are compressed by the pump head and expand further down
the flow path, for example in the column, the volumetric flow is changed
depending on the compressibility of the liquid. Agilent control software
allows specifying pure solvents, pre- mixed solvents and solvent gradients.
Associated Agilent solvent libraries are used by the pump control for
enhanced flow accuracy, which is required for cross- instrument or
cross- system reproducibility and method compatibility.
A high resolution encoder unit is attached to the pump drives, which
divides a single turn into 65000 steps. Each step corresponds to a volume
of about 300 pL, which allows an extremely precise control.

1290 Infinity Quaternary Pump User Manual 13
Introduction 1
Operating Principle
Figure 1 The hydraulic path
Pump head
Inline filter
Jet Weaver (optional)
Pressure sensor
Solvent bottles
Degasser
Multi-channel gradient valve
Inlet weaver
Multi Purpose Valve

14 1290 Infinity Quaternary Pump User Manual
1Introduction
Positions of the Multi Purpose Valve
Positions of the Multi Purpose Valve
The Multi Purpose Valve allows easy software controlled switching between
different modes of operation.
Normal Operating Mode Without Mixer
In normal operating mode, the flow comes from the pump head, passes
the pressure sensor and arrives at the central port of the Multi Purpose
Valve. The flow passes the inline filter and leaves the valve through port 4
to the system (autosampler etc.).
Figure 2 Valve position in normal operating mode without mixer
From pump
Inline filter
To system

1290 Infinity Quaternary Pump User Manual 15
Introduction 1
Positions of the Multi Purpose Valve
Purge Mode
In purge mode, the flow is diverted to the waste container.
Figure 3 Valve position in purge mode
From pump
To waste

16 1290 Infinity Quaternary Pump User Manual
1Introduction
Positions of the Multi Purpose Valve
Normal Operating Mode With Mixer
In this mode, the flow passes an optional Jet Weaver and the inline filter.
This configuration is recommended for special applications which require
an increased mixing efficiency.
Figure 4 Valve position in normal operating mode with mixer
From pump
To system
Inline filter
Jet Weaver

1290 Infinity Quaternary Pump User Manual 17
Introduction 1
Positions of the Multi Purpose Valve
Filter Flush Mode
This mode is used for cleaning the inline filter by back-flushing it. The
flow goes to port 5, passes the inline filter in opposite direction and
leaves to the waste through port 7.
Figure 5 Valve position in filter flush mode
From pump
Inline filter
To waste

18 1290 Infinity Quaternary Pump User Manual
1Introduction
Positions of the Multi Purpose Valve
Service Mode
In service mode, the flow is diverted to port 3, where for example a
restriction capillary can be installed for diagnostic tests.
Figure 6 Valve position in service mode
From pump
Restriction
capillary

1290 Infinity Quaternary Pump User Manual 19
Introduction 1
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.

20 1290 Infinity Quaternary Pump User Manual
1Introduction
System Overview
Figure 7 Leak and waste handling concept (overview - typical stack configuration as an
example)
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