Agilent Technologies 1200 series User manual

Agilent Technologies
Agilent 1200 Series
Thermostatted Column
Compartment
G1316A/G1316B/G1316C
User Manual
1200 Series TCC User Manual

1200 Series TCC User Manual
Notices
© Agilent Technologies, Inc. 1995,
1996-2008
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tronic storage and retrieval or translation
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Manual Part Number
G1316-90011
Edition
09/2008
Printed in Germany
Agilent Technologies
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76337 Waldbronn
Research Use Only
Not for use in Diagnostic Procedures.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
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WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
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Manual Structure
The User Manual G1316-90011 (English)
and its localized versions contain a subset
of the Service Manual and is shipped with
the Thermostatted Column Compartment in
printed matter.
Latest versions of the manuals can be
obtained from the Agilent web.

1200 Series TCC User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1200 Series Thermostatted Column
Compartments (TCC)
•G1316A Agilent 1200 Series TCC
•G1316B Agilent 1200 Series TCC SL
•G1316C Agilent 1200 Series TCC SL Plus
1 Introduction to the Column Compartment
This chapter gives an introduction to the TCC, instrument overview and
internal connectors.
3 Installing the Column Compartment
This chapter describes the installation of the Thermostatted Column
Compartment.
4 How to optimize the Column Compartment
This chapter provides information on how to optimize the Thermostatted
Column Compartement.
5 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
6TestFunctions
This chapter describes the TCC’s built in test functions.

4 1200 Series TCC User Manual
In This Guide…
7 Maintenance
This chapter describes the maintenance of the TCC.
8 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
9 Appendix
This chapter provides addition information on safety, legal and web.

1200 Series TCC User Manual 5
Contents
Contents
1 Introduction to the Column Compartment 9
Main Features 10
System Overview 11
Column-Identification System 13
Column Switching Valve (Optional for G1316A/G1316B SL) 15
Electrical Connections 18
Instrument Layout 21
Agilent Diagnostic Software 22
2 Site Requirements and Specifications 23
Site Requirements and Specifications 24
Physical Specifications 27
Performance Specifications 28
Extended Specifications on G1316B SL/G1316C SL Plus 30
3 Installing the Column Compartment 33
Unpacking the Column Compartment 34
Optimizing the Stack Configuration 35
Installing the Column Compartment 38
Installing Valve Heads (G1316C SL Plus) 42
Flow Connections of the Column Compartment 44
Installation of Heater and Cooling Devices 47
Placing Columns 49
4 How to optimize the Column Compartment 51
Optimizing the Performance of your Column Compartment 52
Using Additional Heater and Cooling Devices 53

6 1200 Series TCC User Manual
Contents
5 Troubleshooting and Diagnostics 55
Overview of the Column Department’s Indicators and Test Functions 56
Status Indicators 57
Available Tests depending on User Interfaces 59
Agilent Diagnostic Software 60
6TestFunctions 61
Thermostat Function Test 62
Pressure Test 65
Column Thermostat Temperature Calibration 66
7 Maintenance 71
Introduction to Maintenance and Repair 72
Warnings and Cautions 73
Overview of Maintenance 75
Cleaning the Column Compartment 76
Changing Column Identification Tags 77
Replacing Head Parts of Column Switching Valve (G1316A/G1316B SL) 79
Adding Heater and Cooling Devices (G1316B SL/G1316C SL Plus) 82
Correcting Leaks 84
Replacing the Column Compartment’s Firmware 85
Replacing Valve Heads (G1316C SL Plus) 86
Preparing the G1316C SL Plus for Transportation 89
8 Parts and Materials for Maintenance 91
Valve Options Overview 92
Heater and Cooling Devices for G1316B SL/G1316C SL Plus 93
Column Switching Valve 2 Position/6 Port (G1316A/G1316B SL) 95
Column Switching Valve 8 Position-9 Port (G1316C SL Plus) 97
Micro Column Switching Valve 2 Postion/6 Port (G1316A/G1316B SL) 99
Micro Column Switching Valve 2 Position/10 Port (G1316A/G1316B SL) 100
Accessory Kits 102
Plastic Parts 109
Leak Parts 110

1200 Series TCC User Manual 7
Contents
9 Appendix 113
Safety Symbols 114
The Waste Electrical and Electronic Equipment Directive 117
Lithium Batteries Information 118
Radio Interference 119
Sound Emission 120
Solvent Information 121
Agilent Technologies on Internet 123

8 1200 Series TCC User Manual
Contents

9
1200 Series TCC User Manual
Agilent Technologies
1
Introduction to the Column
Compartment
Main Features 10
System Overview 11
Column-Identification System 13
Column Switching Valve (Optional for G1316A/G1316B SL) 15
Electrical Connections 18
Serial Number Information 20
Instrument Layout 21
Agilent Diagnostic Software 22
This chapter gives an introduction to the TCC, instrument overview and
internal connectors.

10 1200 Series TCC User Manual
1Introduction to the Column Compartment
Main Features
Main Features
The Agilent 1200 Series thermostatted column compartments are stackable
temperature-controlled column compartments for LC. They are available as
standalone modules or as a component of a Agilent 1200 Series system. They
are used for heating and cooling to meet extreme requirements of retention
time reproducibility.
The main features are:
•Peltier heating and cooling from 10 degrees below ambient up to
80 °C(G1316A) or 100 °C (G1316B SL/G1316C SL Plus) with high heating
and cooling speeds for maximum application flexibility and stability,
•holds up to three 30-cm columns and optimized design gives minimum dead
volumes and maximum efficiency,
•two independently programmable heat exchangers contribute volumes of
only 3 and 6 µl,
•G1316B SL features additional heating and cooling devices for low flow
rates, which reduce the risk of additional dispersion,
•G1316B SL and G1316C SL Plus can be supplemented by a kit to install a
small heat-exchanger with 1.6 µl delay volume to reduce the delay volume.
In additon a cooling device with 1.5 µl is available,
•electronic column-identification module as standard for GLP
documentation of column type, and major column parameters,
•optional high-quality Rheodyne®column switching valves with ceramic
stator-face assemblies for prolonged lifetime.
For specifications, see “Performance Specifications” on page 28.

1200 Series TCC User Manual 11
Introduction to the Column Compartment 1
System Overview
System Overview
The Concept of Heating and Cooling
The design of this thermostatted column compartment uses column heating
and cooling devices with Peltier elements. The solvent entering the column
compartment is heated up or cooled down to a settable temperature with two
low-volume heat exchangers (3 µl on left side, 6 µl on right side), made of a
short piece of capillary 0.17 mm i.d. leading through a heat exchanger. The
heat exchanger is designed such that it can function simultaneously as an air
heater. The shape of the heat exchanger surface allows the area around the
column to be kept at a similar temperature level as the liquid running through
the column. This is done by thermal convection and radiation between the
heat exchanger fins. This design ensures that the column and the solvent
flowing through it are almost at the same temperature.
Actual temperature control is accomplished at the heat exchanger. The solvent
cools down or heats up on its transfer from the heating block to the column
inlet. This depends on several factors: flow rate, setpoint temperature,
ambient temperature and column dimensions.
In a flow-through temperature regulation system, there are necessarily slightly
different temperatures at different positions. If, for example, the temperature
set by the user is 40 °C, then the heat exchanger is regulated to a temperature
40.8 °C which is different by a certain offset (here. 0.8 °C). The solvent
temperature at the column entry would be about 39 °C.
The actual temperature displayed on the user interface is always the derived
temperature taken at the heat exchanger, corrected by the offset explained
above.
Any type of heated column compartment brings one important consequence
for column temperature equilibration. Before an equilibrium is reached, the
whole mass of column, column packing, and solvent volume inside the column
has to be brought to the selected temperature. This depends on several factors:
flow rate, setpoint temperature, ambient temperature and column dimensions.
The higher the flow rate, the faster the column equilibrates (due to
thermostatted mobile phase).

12 1200 Series TCC User Manual
1Introduction to the Column Compartment
System Overview
“Column Thermostat Temperature Calibration” on page 66 shows a setpoint
temperature of 40 °C. Some time after entering the setpoint the heat
exchanger has reached its temperature and the control activity starts. The
TEMPERATURE NOT READY signal will be cancelled 20 seconds after the sensed
temperature was within a range of ± 0.5 °C of the setpoint (other values can be
set via the user interface). However this does not necessarily mean that the
column has already reached the correct temperature. The equilibration of the
column may take longer. Stability of the pressure signal is a good indication
for equilibrium.
Figure 1 Equilibration of Heat Exchanger and Column Temperature
The temperature calibration and verification is described in the Service
Manual.
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1200 Series TCC User Manual 13
Introduction to the Column Compartment 1
Column-Identification System
Column-Identification System
The Agilent 1200 Series thermostatted column compartment is equipped with
a column-identification system. It allows to read and write column-specific
information to and from the column-identification tag.
Figure 2 Column-Identification System
Table 1 on page 13 shows the information that can be stored. The information
fields can be edited via the user interface.
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Table 1 Column-Identification Module Information
Item Example Comment
Product number 79916OD-552
Serial number 950522 Date of manufacturing
Batch number 1675
Geometry [mm] 100 × 2.1
Stationary phase ODS Hypersil
Particle size 10 µm
Number of injections 1267 See Note below.
Maximum pressure allowed [bar] 400

14 1200 Series TCC User Manual
1Introduction to the Column Compartment
Column-Identification System
The number of injections will be updated each run to create a column lifecycle
(history). The user interface allows to edit all information.
Maximum temperature recommended [°C] 70
Maximum pH recommended 12
Column void volume [ml]
Table 1 Column-Identification Module Information
Item Example Comment
NOTE If a column switching valve (see “Column Switching Valve (Optional for G1316A/G1316B
SL)” on page 15) is installed in the module, the update of the number of injections depends
on the position of the column switching valve. For example, if the left column is selected,
the right column is not updated, and vice versa. If no column switching valve is installed
both sides are updated at the same time.

1200 Series TCC User Manual 15
Introduction to the Column Compartment 1
Column Switching Valve (Optional for G1316A/G1316B SL)
Column Switching Valve (Optional for G1316A/G1316B SL)
Figure 3 Location of Column Switching Valve

16 1200 Series TCC User Manual
1Introduction to the Column Compartment
Column Switching Valve (Optional for G1316A/G1316B SL)
Two Column Selection
The valve can select either column 1 or column 2. The offline column is sealed
by connecting head to tail. Switching should be done when the flow is off and
the pressure is zero.
Figure 4 Column 1 Active
Figure 5 Column 2 Active
NOTE Before switching the valve, switch off the pump or set the flow to zero. Keeping the flow on
while the valve is switched can cause exceeding the maximum pressure. This will stop
method or sequence execution.
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1200 Series TCC User Manual 17
Introduction to the Column Compartment 1
Column Switching Valve (Optional for G1316A/G1316B SL)
Precolumn Back-flushing
The sample is injected into series-connected precolumn and analytical
column. After the valve has switched, the analytical column flow continues in
normal direction. Only the precolumn is back-flushed, eluting highly retained
peaks directly to the detector.
Figure 6 Precolumn Back-flushing
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18 1200 Series TCC User Manual
1Introduction to the Column Compartment
Electrical Connections
Electrical Connections
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1200 Series
module data transfer and synchronization.
•One analog output provides signals for integrators or data handling
systems.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
See your software documentation for further information.
•The power input socket accepts a line voltage of 100 – 240 volts AC ± 10%
with a line frequency of 50 or 60 Hz. Maximum power consumption is
220 VA. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply. The security lever at the power input socket prevents the module
cover from being taken off when line power is still connected.
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.

1200 Series TCC User Manual 19
Introduction to the Column Compartment 1
Electrical Connections
Figure 7 Rear View of Oven - Electrical Connections and Label
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20 1200 Series TCC User Manual
1Introduction to the Column Compartment
Electrical Connections
Serial Number Information
The serial number information on the instrument labels provide the following
information:
CCYWWSSSSS Format
CC country of manufacturing
•DE = Germany
•JP = Japan
•CN = China
YWW year and week of last major manufacturing change, e.g. 820
could be week 20 of 1998 or 2008
SSSSS real serial number