Agilent Technologies G1367A User manual

s1
Agilent 1100 Series
Well-plate Sampler &
Micro Well-plate
Sampler
Reference Manual

2 1100 Series WS MWS Reference Manual
Notices
© Agilent Technologies, Inc. 2002
No part of this manual may be reproduced in
any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G1367-90002
Edition
Edition 07/02
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
Software Revision
This guide is valid for A.01.xx revisions of
the Agilent 1100 Series Well-plate Sampler
& Micro Well-plate Sampler software,
where xx refers to minor revisions of the
software that do not affect the technical
accuracy of this guide.
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ject to being changed, without notice,
in future editions. Further, to the max-
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Safety Notices
CAUTION
A CAUTION notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated condi-
tions are fully understood and
met.

1100 Series WS MWS Reference Manual 3
In This Guide…
1 Installing the Sampler
Site requirements and installation of the sampler
2 Optimizing Performance
How to optimize the well-plate sampler and the micro well-plate
sampler to achieve best results
3 Troubleshooting and Test Functions
The modules built-in troubleshooting and test functions
4RepairingtheSampler
Instructions on simple, routine repair procedures as well as
more extensive repairs requiring exchange of internal parts
5 Parts and Materials
Detailed illustrations and lists for identification of parts and
materials
6 Introduction to the Well-plate Sampler
An introduction to the well-plate sampler and thermostatted
well-plate sampler
7 Theory of Operation
Theory of operation of mechanical hardware, electronics, and
instrument interfaces
8 Control Module Screens of the Well-plate Samplers
Introduction to the screens available for operation of the
Agilent 1100 Series well-plate samplers with the control module
9 Specifications
Performance specifications of the well-plate sampler and the
micro well-plate sampler

4 1100 Series WS MWS Reference Manual

1100 Series WS MWS Reference Manual 5
Contents
1 Installing the Sampler
Site Requirements 14
Unpacking the Sampler 17
Optimizing the Stack Configuration 20
Installing the Sampler 24
Installing a Thermostatted Sampler 26
Flow Connections to the Sampler 30
Sample Trays 32
List of recommended Plates and Closing Mat 34
List of Recommended Vials and Caps 36
Configure Well-plate Types 38
Transporting the Sampler 41
2 Optimizing Performance
Optimizing Performance 44
Optimization for Lowest Carry-over 45
Using the Automated Needle Wash 45
Using the Flush Port 46
Using an Injector Program 47
General Recommendation to Lowest Carry-over 48
Fast Injection Cycle and Low Delay Volume 49
Overlapped Injection Mode 49
General Recommendations for Fast Injection Cycle Times 50

6 1100 Series WS MWS Reference Manual
Precise Injection Volume 51
Draw and Eject Speed 51
Choice of Rotor Seal 53
Choice of Seat Capillary 54
3 Troubleshooting and Test Functions
Overview of the Sampler’s Indicators and Test Functions 56
Status Indicators 58
Power Supply Indicator 58
Instrument Status Indicator 59
Error Messages 60
Timeout 61
Shutdown 62
Remote Timeout 63
Synchronization Lost 64
Leak 65
Leak Sensor Open 66
Leak Sensor Short 67
Compensation Sensor Open 68
Compensation Sensor Short 69
Fan Failed 70
Exhaust Fan Failed 71
Front Door Error 72
Side Door Error 73
Arm Movement Failed or Arm Movement Timeout 74
Valve to Bypass Failed 75
Valve to Mainpass Failed 76
Needle Lock Failed 77
Needle to Needle Seat Position 78
Needle Carrier Failed 79
Missing Vial or Missing Wash Vial 80

1100 Series WS MWS Reference Manual 7
Initialization Failed 81
Metering Home Failed 82
Motor Temperature 83
Invalid Vial Position 84
Peristaltic Pump Error 85
Vessel or Wash Vessel Error 86
Vessel Stuck to Needle 87
Maintenance Functions 88
Sample Transport Self Alignment 89
Step Commands 90
Troubleshooting 91
Troubleshooting Guide for the Well Plate Sampler G1367A 93
Turn ON and initialization steps 94
Errors which may occur during the turn ON and initialization
process 95
Instrument logbook errors and step by step repair process 99
1. Fan error (Main fan or Exhaust fan) 99
2. Initialization error 99
3. Metering sensor error 99
4. Rheodyne valve error 100
5. Needle lock error 100
6. Needle into seat error 101
7. Needle / Seat error 102
8. Sample location error 103
Needle centering over the vial or the well 104
4RepairingtheSampler
Introduction into Repairing the Sampler 106
Using the ESD Strap 107
Overview of Main Repair Procedures 108

8 1100 Series WS MWS Reference Manual
Simple Repairs 109
Removing the Needle Assembly 110
Installing the Needle Assembly 112
Removing the Needle Carrier Assembly 114
Installing the Needle Carrier Assembly 115
Exchange the Needle Seat Assembly (G1367-87101) on the
G1367A/68A Samplers 116
Exchange the Needle Seat ( G1377-87101) on the G1377A/78A
Samplers 117
Exchange the Seat Capillary (G1375-87317/G1375-87300) on the
G1377A/78A Samplers 118
Stator Face 119
Rotor Seal 120
Metering Seal and Plunger 122
Removing the Loop Capillary 124
Installing the Loop Capillary 126
Peristaltic Pump 128
Interface Board 129
Exchanging Internal Parts 130
Assembling the Main Cover 131
Light protection kit installation 131
To p Co v e r a n d F o am 132
Removing the Top Cover and Foam 132
Installing the Top Cover and Foam. 132
Transport Assembly 133
Removing the sample transport 133
Installing the sample transport 133
Sampling Unit 134
Removing the sampling unit 134
Installing the sampling unit 135
Analytical head 136
Removing the analytical head 136
Installing the analytical head 136

1100 Series WS MWS Reference Manual 9
Peristaltic Pump Motor 137
Removing the peristaltic pump motor 137
Installing the peristaltic pump motor 138
Injection-Valve Assembly 139
Removing the injection valve assembly 139
Installing the injection valve assembly 140
Metering-Drive Motor and Belt 141
Removing the metering drive motor and belt 141
Installing the metering drive motor and belt 142
Needle-lock Motor and Belt 143
Removing the needle lock motor and belt 143
Installing the needle lock motor and belt 143
Main Fan 144
Removing the main fan 144
Installing the main fan 144
Exhaust Fan 145
Removing the exhaust fan 145
Installing the exhaust fan 145
MTP Main Board 146
SUD Board 149
SLS Board 151
Power Supply 152
Leak Sensor 154
Replacing the Autosampler Firmware 155
5 Parts and Materials
Sampler Main Assemblies 158
Vial Trays 160
Sampling Unit Assembly 162
Analytical-Head Assembly 165
Injection-Valve Assembly 167

10 1100 Series WS MWS Reference Manual
Sheet Metal Kit 169
Cover Parts 170
Foam Parts 171
Power and Status Light Pipes 172
Leak System Parts 173
Well-plate Sampler Accessory Kit G1367-68705 174
Micro Well-plate Sampler Accessory Kit G1377-68705 175
Multi-Draw Kit G1313-68711 (only for G1367A/68A) 176
Well-plate Sampler Thermostat 177
Cable Overview 178
Analog Cables 180
Remote Cables 183
BCD Cables 188
Auxiliary Cable 191
CAN Cable 192
External Contact Cable 193
RS-232 Cable Kit 194
LAN Cables 195
6 Introduction to the Well-plate Sampler
Introduction to the Well-plate Sampler 198
Sampling Sequence 200
Injection Sequence 202
Sampling Unit 204
Analytical head 204
Injection-Valve 205
Needle Flush Station 206
Needle Lock 206

1100 Series WS MWS Reference Manual 11
Needle/Sample Transport Assembly 207
Advanced Operating Modes 209
Early Maintenance Feedback (EMF) 211
EMF Counters 211
Using the EMF Counters 212
Setting the EMF Limits 212
Electrical Connections 213
7 Theory of Operation
Autosampler Control and Electronics 216
Position and Movement Sensors 217
Microtiter Plate Board (MTP) 218
Autosampler-Specific Electronics 219
Firmware Description 223
Firmware Updates 224
Optional Interface Boards 225
BCD Board 225
LAN Board 227
Interfaces 228
Analog Signal Output 229
GPIB Interface 229
CAN Interface 229
Remote Interface 230
RS-232C 231
Setting the 8-bit Configuration Switch 233
GPIB Default Addresses 234
Communication Settings for RS-232C Communication 235
Forced Cold Start Settings 236
Stay-Resident Settings 237

12 1100 Series WS MWS Reference Manual
The Main Power Supply Assembly 238
8 Control Module Screens of the Well-plate Samplers
Major keys on the Agilent 1100 Control Module 242
Screens available from the Analysis view 243
Screens available from the System view 259
9 Specifications
Performance Specifications 274
ASafetyInformation
General Safety Information 278
Index

13
Agilent 1100 Series Well-plate Sampler & Micro Well-plate Sampler
Reference Manual
Agilent Technologies
1
Installing the Sampler
Site Requirements 14
Unpacking the Sampler 17
Optimizing the Stack Configuration 20
Installing the Sampler 24
Installing a Thermostatted Sampler 26
Flow Connections to the Sampler 30
Sample Trays 32
List of recommended Plates and Closing Mat 34
List of Recommended Vials and Caps 36
Configure Well-plate Types 38
Tra ns po rt ing the Sampler 41

14 1100 Series WS MWS Reference Manual
1Installing the Sampler
Site Requirements
A suitable site environment is important to ensure optimal performance of the
autosampler.
Power Consideration
The autosampler power supply has wide-ranging capability (see Table 1 on
page 16). Consequently there is no voltage selector in the rear of the
autosampler. There are also no externally accessible fuses, because automatic
electronic fuses are implemented in the power supply.
The thermostatted autosampler comprises two modules, the sampler (G1367A
or G1377A) and the thermostat (G1330A). Both modules have a separate
power supply and a power plug for the line connections. The two modules are
connected by a control cable and both are turned on by the sampler module.
The thermostat power supply has two externally accessible fuses.
WARNING To disconnect the autosampler from line power, unplug the power cord. The power
supply still uses some power, even if the power switch on the front panel is turned
off.
WARNING To disconnect the thermostatted autosampler from line power, unplug the power
cord from the autosampler and the ALS thermostat. The power supplies still use
some power, even if the power switch on the front panel is turned off. Please make
sure that it is always possible to access the power plug.
WARNING Shock hazard or damage of your instrumentation can result if the devices are
connected to a line voltage higher than specified.

Installing the Sampler 1
1100 Series WS MWS Reference Manual 15
Power Cords
Your autosampler is delivered with a power cord which matches the wall
socket of your particular country or region. The plug on the power cord which
connects to the rear of the instrument is identical for all types of power cord.
Bench Space
The autosampler dimensions and weight (see Table 1 on page 16) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 2.5 cm (1.0 inch) of space on either side, and
approximately 8 cm (3.1 inches) at the rear for the circulation of air, and room
for electrical connections. Ensure the autosampler is installed in a horizontal
position.
The thermostatted sampler dimensions and weight (see Table 1 on page 16)
allow the instrument to be placed on almost any laboratory bench. The
instrument requires an additional 25 cm (10 inches) of space on either side for
the circulation of air, and approximately 8 cm (3.1 inches) at the rear for
electrical connections. Ensure the autosampler is installed in a horizontal
position.
If a complete Agilent 1100 Series system is to be installed on the bench, make
sure that the bench is designed to carry the weight of all the modules. For a
complete system including the thermostatted sampler it is recommended to
position the modules in two stacks, see “Recommended Stack Configuration -
Well-plate Sampler (Front View)" on page 20. Make sure that in this
configuration there is 25 cm (10 inches) space on either side of the
thermostatted sampler for the circulation of air.
WARNING Never operate your instrumentation from a power outlet that has no ground
connection. Never use a power cord other than the power cord designed for your
region.
WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

16 1100 Series WS MWS Reference Manual
1Installing the Sampler
Environment
Your autosampler will work within specifications at ambient temperatures
and relative humidity as described in Table 1.
CAUTION Do not store, ship or use your autosampler under conditions where temperature
fluctuations may cause condensation within the autosampler. Condensation will
damage the system electronics. If your autosampler was shipped in cold weather, leave
it in its box, and allow it to warm up slowly to room temperature to avoid condensation.
Table 1 Physical Specifications - sampler (G1367A / G1377A)
Type Specification Comments
Weight 15.5 kg (34.2 lbs)
Dimensions
(height × width × depth)
200 × 345 × 440 mm
(8 × 13.5 × 17 inches)
Line voltage 100 – 240 VAC, ±10 % Wide-ranging capability
Line frequency 50 or 60 Hz, ±5%
Power consumption (apparent power) 300 VA Maximum
Power consumption (active power) 200 W Maximum
Ambient operating temperature 4 to 55 ∞C (41 to 131 ∞F)
Ambient non-operating temperature -40 to 70 ∞C (-4 to 158 ∞F)
Humidity < 95 %, at 25 to 40 ∞C (77 to 104 ∞F) Non-condensing
Operating Altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storing the
autosampler
Safety standards: IEC, CSA, UL Installation Category II, Pollution Degree 2

Installing the Sampler 1
1100 Series WS MWS Reference Manual 17
Unpacking the Sampler
Damaged Packaging
Upon receipt of your autosampler, inspect the shipping containers for any
signs of damage. If the containers or cushioning material are damaged, save
them until the contents have been checked for completeness and the
autosampler has been mechanically and electrically checked. If the shipping
container or cushioning material is damaged, notify the carrier and save the
shipping material for the carrier‘s inspection.
Delivery Checklist
Ensure all parts and materials have been delivered with the autosampler. For
this compare the shipment content with the checklist included in each
instrument box. Please report missing or damaged parts to your local Agilent
Technologies sales and service office.
CAUTION If you need to ship the autosampler at a later date, always use the shipping protection
foam parts (see “Transporting the Sampler" on page 41).
CAUTION If there are signs of damage to the autosampler, please do not attempt to install the
autosampler.

18 1100 Series WS MWS Reference Manual
1Installing the Sampler
Accessory Kits
Each shipment contents an Accessory kit with the necessary tools to install
the system and to have an operating system.
•The Accessory kit (G1367-68705) shown in Table 2 is shipped with the
(G1367A) well-plate and the (G1368A) thermostatted well-plate samplers.
•The Accessory kit (G1377-68705) shown in Table 3 on page 19 is shipped
with the (G1377A) micro well-plate and the (G1378A) thermostatted micro
well-plate samplers.
Table 2 Well-plate Sampler Accessory Kit Contents G1367-68705
Description Quantity Part Number
Capillary sampler-column (380 mm, 0.17 mm ID) 1 01090-87306
96 well-plate 0.5 ml, PP (pack of 10) 1 5042-1386
Tubing assembly 1 5063-6527
Filter kit 1 5064-8240
CAN cable, 1 m 1 5181-1519
Vials, screw cap 100/pk 1 5182-0716
Blue screw caps 100/pk 1 5182-0717
Valve catalog 1 5988-2999
Hex key 9/64 inch (for injection-valve screws) 1 8710-0060
Wrenches 1/4 – 5/16 inch 2 8710-0510
Rheotool socket wrench 1/4 inch 1 8710-2391
Hex key 4.0 mm, 15 cm long, T-handle 1 8710-2392
Hex key 9/64 inch, 15 cm long, T-handle 1 8710-2394
Hex key 2.0 mm 1 8710-2438
ESD wrist strap 1 9300-1408
Air channel adapter 1 G1329-43200
Capillary pump-sampler (900 mm, 0.17 mm ID) 1 G1329-87300
WPS leak kit 1 G1367-60006

Installing the Sampler 1
1100 Series WS MWS Reference Manual 19
Table 3 Micro well-plate Sampler Accessory Kit Contents G1377-68705
Description Quantity Part Number
96 well-plate 0.5 ml, PP (pack of 10) 1 5042-1386
Tubing assembly 1 5063-6527
Filter kit 1 5064-8240
CAN cable, 1 m 1 5181-1519
Vials, screw cap 100/pk 1 5182-0716
Blue screw caps 100/pk 1 5182-0717
Valve catalog 1 5988-2999
Hex key 9/64 inch (for injection-valve screws) 1 8710-0060
Wrenches 1/4 – 5/16 inch 2 8710-0510
Wrench 4.0 mm open end 1 8710-1534
Rheotool socket wrench 1/4 inch 1 8710-2391
Hex key 4.0 mm, 15 cm long, T-handle 1 8710-2392
Hex key 9/64 inch, 15 cm long, T-handle 1 8710-2394
Hex key 2.5 mm, 15 cm long, straight handle 1 8710-2412
Hex key 2.0 mm 1 8710-2438
ESD wrist strap 1 9300-1408
Torque adapter 1 G1315-45003
Air channel adapter 1 G1329-43200
Capillary sampler-column (500 mm, 0.05 mm ID) 1 G1375-87304
40 µl Loop capillary 1 G1377-87300
WPS leak kit 1 G1367-60006

20 1100 Series WS MWS Reference Manual
1Installing the Sampler
Optimizing the Stack Configuration
If your autosampler is part of a system, you can ensure optimum performance,
ensuring minimum delay volume by installing the following configuration.
Figure 1 and Figure 2 on page 21 show the configuration recommended for the
sampler. Figure 3 on page 22 and Figure 4 on page 23 show the configuration
recommended for the thermostatted sampler.
Figure 1 Recommended Stack Configuration - Well-plate Sampler (Front View)
Solvent cabinet
Vacuum degasser
Pump
Column compartment
Well-plate sampler
Detector Control module