Agilent Technologies G1367A User manual

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Agilent 1100 Series
Well-plate Sampler &
Micro Well-plate
Sampler
Reference Manual

2 1100 Series WS MWS Reference Manual
Notices
© Agilent Technologies, Inc. 2002
No part of this manual may be reproduced in
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tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G1367-90002
Edition
Edition 07/02
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
Software Revision
This guide is valid for A.01.xx revisions of
the Agilent 1100 Series Well-plate Sampler
& Micro Well-plate Sampler software,
where xx refers to minor revisions of the
software that do not affect the technical
accuracy of this guide.
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met.

1100 Series WS MWS Reference Manual 3
In This Guide…
1 Installing the Sampler
Site requirements and installation of the sampler
2 Optimizing Performance
How to optimize the well-plate sampler and the micro well-plate
sampler to achieve best results
3 Troubleshooting and Test Functions
The modules built-in troubleshooting and test functions
4RepairingtheSampler
Instructions on simple, routine repair procedures as well as
more extensive repairs requiring exchange of internal parts
5 Parts and Materials
Detailed illustrations and lists for identification of parts and
materials
6 Introduction to the Well-plate Sampler
An introduction to the well-plate sampler and thermostatted
well-plate sampler
7 Theory of Operation
Theory of operation of mechanical hardware, electronics, and
instrument interfaces
8 Control Module Screens of the Well-plate Samplers
Introduction to the screens available for operation of the
Agilent 1100 Series well-plate samplers with the control module
9 Specifications
Performance specifications of the well-plate sampler and the
micro well-plate sampler

4 1100 Series WS MWS Reference Manual

1100 Series WS MWS Reference Manual 5
Contents
1 Installing the Sampler
Site Requirements 14
Unpacking the Sampler 17
Optimizing the Stack Configuration 20
Installing the Sampler 24
Installing a Thermostatted Sampler 26
Flow Connections to the Sampler 30
Sample Trays 32
List of recommended Plates and Closing Mat 34
List of Recommended Vials and Caps 36
Configure Well-plate Types 38
Transporting the Sampler 41
2 Optimizing Performance
Optimizing Performance 44
Optimization for Lowest Carry-over 45
Using the Automated Needle Wash 45
Using the Flush Port 46
Using an Injector Program 47
General Recommendation to Lowest Carry-over 48
Fast Injection Cycle and Low Delay Volume 49
Overlapped Injection Mode 49
General Recommendations for Fast Injection Cycle Times 50

6 1100 Series WS MWS Reference Manual
Precise Injection Volume 51
Draw and Eject Speed 51
Choice of Rotor Seal 53
Choice of Seat Capillary 54
3 Troubleshooting and Test Functions
Overview of the Sampler’s Indicators and Test Functions 56
Status Indicators 58
Power Supply Indicator 58
Instrument Status Indicator 59
Error Messages 60
Timeout 61
Shutdown 62
Remote Timeout 63
Synchronization Lost 64
Leak 65
Leak Sensor Open 66
Leak Sensor Short 67
Compensation Sensor Open 68
Compensation Sensor Short 69
Fan Failed 70
Exhaust Fan Failed 71
Front Door Error 72
Side Door Error 73
Arm Movement Failed or Arm Movement Timeout 74
Valve to Bypass Failed 75
Valve to Mainpass Failed 76
Needle Lock Failed 77
Needle to Needle Seat Position 78
Needle Carrier Failed 79
Missing Vial or Missing Wash Vial 80

1100 Series WS MWS Reference Manual 7
Initialization Failed 81
Metering Home Failed 82
Motor Temperature 83
Invalid Vial Position 84
Peristaltic Pump Error 85
Vessel or Wash Vessel Error 86
Vessel Stuck to Needle 87
Maintenance Functions 88
Sample Transport Self Alignment 89
Step Commands 90
Troubleshooting 91
Troubleshooting Guide for the Well Plate Sampler G1367A 93
Turn ON and initialization steps 94
Errors which may occur during the turn ON and initialization
process 95
Instrument logbook errors and step by step repair process 99
1. Fan error (Main fan or Exhaust fan) 99
2. Initialization error 99
3. Metering sensor error 99
4. Rheodyne valve error 100
5. Needle lock error 100
6. Needle into seat error 101
7. Needle / Seat error 102
8. Sample location error 103
Needle centering over the vial or the well 104
4RepairingtheSampler
Introduction into Repairing the Sampler 106
Using the ESD Strap 107
Overview of Main Repair Procedures 108

8 1100 Series WS MWS Reference Manual
Simple Repairs 109
Removing the Needle Assembly 110
Installing the Needle Assembly 112
Removing the Needle Carrier Assembly 114
Installing the Needle Carrier Assembly 115
Exchange the Needle Seat Assembly (G1367-87101) on the
G1367A/68A Samplers 116
Exchange the Needle Seat ( G1377-87101) on the G1377A/78A
Samplers 117
Exchange the Seat Capillary (G1375-87317/G1375-87300) on the
G1377A/78A Samplers 118
Stator Face 119
Rotor Seal 120
Metering Seal and Plunger 122
Removing the Loop Capillary 124
Installing the Loop Capillary 126
Peristaltic Pump 128
Interface Board 129
Exchanging Internal Parts 130
Assembling the Main Cover 131
Light protection kit installation 131
To p Co v e r a n d F o am 132
Removing the Top Cover and Foam 132
Installing the Top Cover and Foam. 132
Transport Assembly 133
Removing the sample transport 133
Installing the sample transport 133
Sampling Unit 134
Removing the sampling unit 134
Installing the sampling unit 135
Analytical head 136
Removing the analytical head 136
Installing the analytical head 136

1100 Series WS MWS Reference Manual 9
Peristaltic Pump Motor 137
Removing the peristaltic pump motor 137
Installing the peristaltic pump motor 138
Injection-Valve Assembly 139
Removing the injection valve assembly 139
Installing the injection valve assembly 140
Metering-Drive Motor and Belt 141
Removing the metering drive motor and belt 141
Installing the metering drive motor and belt 142
Needle-lock Motor and Belt 143
Removing the needle lock motor and belt 143
Installing the needle lock motor and belt 143
Main Fan 144
Removing the main fan 144
Installing the main fan 144
Exhaust Fan 145
Removing the exhaust fan 145
Installing the exhaust fan 145
MTP Main Board 146
SUD Board 149
SLS Board 151
Power Supply 152
Leak Sensor 154
Replacing the Autosampler Firmware 155
5 Parts and Materials
Sampler Main Assemblies 158
Vial Trays 160
Sampling Unit Assembly 162
Analytical-Head Assembly 165
Injection-Valve Assembly 167

10 1100 Series WS MWS Reference Manual
Sheet Metal Kit 169
Cover Parts 170
Foam Parts 171
Power and Status Light Pipes 172
Leak System Parts 173
Well-plate Sampler Accessory Kit G1367-68705 174
Micro Well-plate Sampler Accessory Kit G1377-68705 175
Multi-Draw Kit G1313-68711 (only for G1367A/68A) 176
Well-plate Sampler Thermostat 177
Cable Overview 178
Analog Cables 180
Remote Cables 183
BCD Cables 188
Auxiliary Cable 191
CAN Cable 192
External Contact Cable 193
RS-232 Cable Kit 194
LAN Cables 195
6 Introduction to the Well-plate Sampler
Introduction to the Well-plate Sampler 198
Sampling Sequence 200
Injection Sequence 202
Sampling Unit 204
Analytical head 204
Injection-Valve 205
Needle Flush Station 206
Needle Lock 206