Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
LC Injectors HTC/HTS
User Manual

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Notices
© Agilent Technologies, Inc. 2009
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G4270-90000
Edition
12/2009
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
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ject to being changed, without notice,
in future editions. Further, to the max-
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either express or implied, with regard
to this manual and any information
contained herein, including but not
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ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
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arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
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or as a “commercial item” as defined in FAR
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.
For Research Use Only

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 3
Contents
Contents
1 General Information 5
Safety Information 6
How to Use this Manual 12
2 LC Injector HTC/HTS Operating Instructions 13
Using the Control Terminal 15
Methods 18
Job and Job Queue 21
Utility Functions 25
Logfile 35
Info Functions 36
Setup Functions 40
3 LC Injector HTC/HTS Description and Installation 43
General System Overview 45
Installation 50
Object Positions 87
Injection Valve 105
Interfacing the LC Injector HTC/HTS to other Devices 115
PAL Loader Program 118
Establishing Communication with Computer 119
Special Functions 125
Troubleshooting 146
Replacing Parts 166
Maintaining the LC Injector HTC/HTS System 172

4 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Contents
4 Appendices 175
Definition of Terms 176
Conventions of Naming 179
Injection Cycles 180
DLW Principles and Operating Details 184
Software Flow Chart 232
External Connectors 239

5
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Agilent Technologies
1
General Information
Safety Information 6
How to Use this Manual 12

6 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
Safety Information
Safety Information
General
Considerations
The LC Injector HTC/HTS System User Manual and the corresponding
Addendum for a specific module must be consulted by the user under all
circumstances before a unit is put in use.
Changes or modifications to this unit not expressly approved by the party
responsible for compliance could void the user’s authority to operate the
equipment.
The user shall be made aware that if the equipment is used in a manner not
specified by the manufacturer, the protection provided by the equipment may
be impaired.
When using the LC Injector HTC/HTS System, follow the generally accepted
procedures for quality control and methods development.
When you use the LC Injector HTC/HTS System in the field of
chromatographic analysis and you observe a change in the retention of a
particular compound, in the resolution between two compounds, or in peak
shape, immediately determine the reason for the changes. Until you determine
the cause of a change, do not rely on the separation results.
Electrical
Hazards
Every analytical instrument has specific hazards, so be sure to read and
comply with the following precautions. They will help ensure the safe,
long-term use of your LC Injector HTC/HTS System.
The Installation Category (Over voltage Category) for this instrument is Level
II. The Level II Category pertains to equipment that receives its electrical
power from the local level, such as an electrical wall outlet.
Only use fuses of the type and current rating specified. Do not use repaired
fuses and do not short-circuit the fuse holder.

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 7
General Information 1
Safety Information
The supplied power cord must be inserted into a power outlet with a
protective earth contact (ground). When using an extension cord, make sure
that the cord also has an earth contact.
Do not change the external or internal grounding connections. Tampering
with or disconnecting these connections could endanger you and/or damage
the LC Injector HTC/HTS System.
The instrument is properly grounded in accordance with these regulations
when shipped. You do not need to make any changes to the electrical
connections or the instrument's chassis to ensure safe operation.
The combination of a LC Injector HTC/HTS System with a LC/MS System does
require the safety measure as described by the LC/MS System manufacturer.
Detailed instructions for the safety grounding on the LC/MS system are
outlined in the corresponding operating/installation manual.
Agilent Technologies recommends to use a grounding cable connected on one
side at the Injection Valve, Loop or any other suitable direct metallic contact
and the other side at an appropriate grounding point at the LC/MS System.
This supplementary grounding measure will support the safety strategy of the
LC/MS System manufacturer.
Do not turn the instrument on if you suspect that it has incurred any kind of
electrical damage. Instead disconnect the power cord and contact a Agilent
Technologies representative for a product evaluation. Do not attempt to use
the instrument until it has been evaluated. Electrical damage may have
occurred if the LC Injector HTC/HTS System shows visible signs of damage,
exposure to any liquids or has been transported under severe stress.
Damage can also result if the instrument is stored for prolonged periods under
unfavorable conditions (e.g. subjected to heat, water, etc.).

8 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
Safety Information
In any case disconnect the power cord(s) from the power supply or from the
different power supplies if optional devices are installed before attempting
any type of maintenance.
Capacitors inside the instrument may still be charged even if the instrument is
turned off.
To avoid damaging electrical parts, do not disconnect an electrical assembly
while power is applied to the LC Injector HTC/HTS system. Once the power is
turned off, wait approximately 30 seconds before you disconnect an assembly.
The instrument includes a number of integrated circuits. These circuits may
be damaged if exposed to excessive line voltage fluctuations and/or power
surges.
Never try to repair or replace any components of the instrument that is not
described in this manual without the assistance of a Agilent Technologies
representative.
There are no operator-serviceable or replaceable parts inside the power
supply(ies) or in the LC Injector HTC/HTS System. If a power supply is not
functioning, contact a Agilent Technologies representative.
The power supply for the LC Injector HTC/HTS Instrument has the symbols
1/0 on the label for the power switch to switch ON/OFF.
Any additional power supply for other devices like, Cooled Stack or a Valve
Module shows the symbols as shown below on the label for the power switch:
The symbols shall warn the user that in a emergency case more than one
power supply has to be turned OFF or more than one power cord has to be
pulled from power supply or from the wall outlet to shut down the complete
LC Injector HTC/HTS System.

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 9
General Information 1
Safety Information
If the basic LC Injector HTC/HTS System is installed, than a single power
supply is installed only. Turning OFF the power supply or pulling this single
power cord in an emergency case will stop the complete LC Injector HTC/HTS
System.
It is important that the power supply (ies) are in a location where the power
ON and OFF switch is accessible and easy to operate, and where it is possible
to unplug the AC power cord from the power supply/wall outlet in case of
emergency.
Other Hazards
To avoid injury during LC Injector HTC/HTS System operation, keep your
hands away from the syringe.
Do not operate the LC Injector HTC/HTS System without the safety shield. The
safety shield must be installed for safe operation.
To avoid injury, observe safe laboratory practice when you handle solvents,
change tubing, or operate the LC Injector HTC/HTS System. Know the physical
and chemical properties of the solvents you use. See the Material Safety Sheets
from the manufacturer for the solvents in use.
Use caution when working with any polymer tubing under pressure:
•Always wear eye protection when near pressurized polymer tubing.
•Do not use polymer tubing that has been severely stressed or kinked.
•Do not use polymer tubing, in particular not PEEK or Tefzel tubing, with
Tetrahydrofuran (THF), Dimethylsulfoxid (DMSO), chlorinated organic
solvents, concentrated mineral acids, such as Nitric, Phosphoric or Sulfuric
acids, or any related compounds to above listings.

10 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
Safety Information
Do not use vials without a sealing cap, microtiter or deepwell plates without a
plate seal. Vapor phase from organic solvents can be hazardous and
flammable. Acidic vapor phase can cause corrosion to critical mechanical
parts.
Disposal
Do not dispose of this equipment or parts thereof unsorted in municipal
waste. Follow local municipal waste ordinances for proper disposal provisions
to reduce the environmental impact of waste electrical and electronic
equipment (WEEE). European Union customers: Call your local customer
service representative responsible for the PAL System for complimentary
equipment pick-up and recycling.
Lithium battery
An onboard lithium battery buffers the electronic memories, when the
instrument is turned off. Replace it only with the same or equivalent type
recommended by the equipment manufacturer.
Battery: Panasonic VL 2330, soldered directly on the electronic board.
Discharged lithium batteries shall be disposed off locally according to national
waste disposal regulations for batteries.
There are no operator-serviceable parts on the electronic boards. If an
electronic board fails, contact a Agilent Technologies representative.