Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
LC Injectors HTC/HTS
User Manual

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Notices
© Agilent Technologies, Inc. 2009
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G4270-90000
Edition
12/2009
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
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The hardware and/or software described in
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.
For Research Use Only

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 3
Contents
Contents
1 General Information 5
Safety Information 6
How to Use this Manual 12
2 LC Injector HTC/HTS Operating Instructions 13
Using the Control Terminal 15
Methods 18
Job and Job Queue 21
Utility Functions 25
Logfile 35
Info Functions 36
Setup Functions 40
3 LC Injector HTC/HTS Description and Installation 43
General System Overview 45
Installation 50
Object Positions 87
Injection Valve 105
Interfacing the LC Injector HTC/HTS to other Devices 115
PAL Loader Program 118
Establishing Communication with Computer 119
Special Functions 125
Troubleshooting 146
Replacing Parts 166
Maintaining the LC Injector HTC/HTS System 172

4 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Contents
4 Appendices 175
Definition of Terms 176
Conventions of Naming 179
Injection Cycles 180
DLW Principles and Operating Details 184
Software Flow Chart 232
External Connectors 239

5
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Agilent Technologies
1
General Information
Safety Information 6
How to Use this Manual 12

6 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
Safety Information
Safety Information
General
Considerations
The LC Injector HTC/HTS System User Manual and the corresponding
Addendum for a specific module must be consulted by the user under all
circumstances before a unit is put in use.
Changes or modifications to this unit not expressly approved by the party
responsible for compliance could void the user’s authority to operate the
equipment.
The user shall be made aware that if the equipment is used in a manner not
specified by the manufacturer, the protection provided by the equipment may
be impaired.
When using the LC Injector HTC/HTS System, follow the generally accepted
procedures for quality control and methods development.
When you use the LC Injector HTC/HTS System in the field of
chromatographic analysis and you observe a change in the retention of a
particular compound, in the resolution between two compounds, or in peak
shape, immediately determine the reason for the changes. Until you determine
the cause of a change, do not rely on the separation results.
Electrical
Hazards
Every analytical instrument has specific hazards, so be sure to read and
comply with the following precautions. They will help ensure the safe,
long-term use of your LC Injector HTC/HTS System.
The Installation Category (Over voltage Category) for this instrument is Level
II. The Level II Category pertains to equipment that receives its electrical
power from the local level, such as an electrical wall outlet.
Only use fuses of the type and current rating specified. Do not use repaired
fuses and do not short-circuit the fuse holder.

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 7
General Information 1
Safety Information
The supplied power cord must be inserted into a power outlet with a
protective earth contact (ground). When using an extension cord, make sure
that the cord also has an earth contact.
Do not change the external or internal grounding connections. Tampering
with or disconnecting these connections could endanger you and/or damage
the LC Injector HTC/HTS System.
The instrument is properly grounded in accordance with these regulations
when shipped. You do not need to make any changes to the electrical
connections or the instrument's chassis to ensure safe operation.
The combination of a LC Injector HTC/HTS System with a LC/MS System does
require the safety measure as described by the LC/MS System manufacturer.
Detailed instructions for the safety grounding on the LC/MS system are
outlined in the corresponding operating/installation manual.
Agilent Technologies recommends to use a grounding cable connected on one
side at the Injection Valve, Loop or any other suitable direct metallic contact
and the other side at an appropriate grounding point at the LC/MS System.
This supplementary grounding measure will support the safety strategy of the
LC/MS System manufacturer.
Do not turn the instrument on if you suspect that it has incurred any kind of
electrical damage. Instead disconnect the power cord and contact a Agilent
Technologies representative for a product evaluation. Do not attempt to use
the instrument until it has been evaluated. Electrical damage may have
occurred if the LC Injector HTC/HTS System shows visible signs of damage,
exposure to any liquids or has been transported under severe stress.
Damage can also result if the instrument is stored for prolonged periods under
unfavorable conditions (e.g. subjected to heat, water, etc.).

8 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
Safety Information
In any case disconnect the power cord(s) from the power supply or from the
different power supplies if optional devices are installed before attempting
any type of maintenance.
Capacitors inside the instrument may still be charged even if the instrument is
turned off.
To avoid damaging electrical parts, do not disconnect an electrical assembly
while power is applied to the LC Injector HTC/HTS system. Once the power is
turned off, wait approximately 30 seconds before you disconnect an assembly.
The instrument includes a number of integrated circuits. These circuits may
be damaged if exposed to excessive line voltage fluctuations and/or power
surges.
Never try to repair or replace any components of the instrument that is not
described in this manual without the assistance of a Agilent Technologies
representative.
There are no operator-serviceable or replaceable parts inside the power
supply(ies) or in the LC Injector HTC/HTS System. If a power supply is not
functioning, contact a Agilent Technologies representative.
The power supply for the LC Injector HTC/HTS Instrument has the symbols
1/0 on the label for the power switch to switch ON/OFF.
Any additional power supply for other devices like, Cooled Stack or a Valve
Module shows the symbols as shown below on the label for the power switch:
The symbols shall warn the user that in a emergency case more than one
power supply has to be turned OFF or more than one power cord has to be
pulled from power supply or from the wall outlet to shut down the complete
LC Injector HTC/HTS System.

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 9
General Information 1
Safety Information
If the basic LC Injector HTC/HTS System is installed, than a single power
supply is installed only. Turning OFF the power supply or pulling this single
power cord in an emergency case will stop the complete LC Injector HTC/HTS
System.
It is important that the power supply (ies) are in a location where the power
ON and OFF switch is accessible and easy to operate, and where it is possible
to unplug the AC power cord from the power supply/wall outlet in case of
emergency.
Other Hazards
To avoid injury during LC Injector HTC/HTS System operation, keep your
hands away from the syringe.
Do not operate the LC Injector HTC/HTS System without the safety shield. The
safety shield must be installed for safe operation.
To avoid injury, observe safe laboratory practice when you handle solvents,
change tubing, or operate the LC Injector HTC/HTS System. Know the physical
and chemical properties of the solvents you use. See the Material Safety Sheets
from the manufacturer for the solvents in use.
Use caution when working with any polymer tubing under pressure:
•Always wear eye protection when near pressurized polymer tubing.
•Do not use polymer tubing that has been severely stressed or kinked.
•Do not use polymer tubing, in particular not PEEK or Tefzel tubing, with
Tetrahydrofuran (THF), Dimethylsulfoxid (DMSO), chlorinated organic
solvents, concentrated mineral acids, such as Nitric, Phosphoric or Sulfuric
acids, or any related compounds to above listings.

10 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
Safety Information
Do not use vials without a sealing cap, microtiter or deepwell plates without a
plate seal. Vapor phase from organic solvents can be hazardous and
flammable. Acidic vapor phase can cause corrosion to critical mechanical
parts.
Disposal
Do not dispose of this equipment or parts thereof unsorted in municipal
waste. Follow local municipal waste ordinances for proper disposal provisions
to reduce the environmental impact of waste electrical and electronic
equipment (WEEE). European Union customers: Call your local customer
service representative responsible for the PAL System for complimentary
equipment pick-up and recycling.
Lithium battery
An onboard lithium battery buffers the electronic memories, when the
instrument is turned off. Replace it only with the same or equivalent type
recommended by the equipment manufacturer.
Battery: Panasonic VL 2330, soldered directly on the electronic board.
Discharged lithium batteries shall be disposed off locally according to national
waste disposal regulations for batteries.
There are no operator-serviceable parts on the electronic boards. If an
electronic board fails, contact a Agilent Technologies representative.

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 11
General Information 1
Safety Information
Table 1 Commonly Used Symbols
Symbol Description
Caution or refer to User Manual
Caution, Risk of Needle-Stick Puncture
Caution, Hot Surface or High Temperature
Direct Current
Alternating Current
Protective Conductor Terminal, Ground
Fuse
I Electrical Power ON.
Used with Main LC Injector HTC/HTS Power Supply.
O Electrical Power OFF.
Used with Main LC Injector HTC/HTS Power Supply.
Electrical Power ON for Only Part of the System.
Used with Optional Device(s)
Electrical Power OFF for Only Part of the System.
Used with Optional Device(s)
Caution, Risk of Electrical shock (high voltage)
Disposal, Do not dispose in municipal waste. Follow local waste regulations to reduce electrical and
electronic waste (WEEE).

12 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
1General Information
How to Use this Manual
How to Use this Manual
The manual is divided into three major sections
•“LC Injector HTC/HTS Operating Instructions” on page 13)
•“LC Injector HTC/HTS Description and Installation” on page 43)
•“Appendices” on page 175
The "LC Injector HTC/HTS Operating instructions" are intended for infrequent
LC Injector HTC/HTS users or new users that are experienced in using
automated systems to perform existing analytical methods.
Users who are installing a LC Injector HTC/HTS system, LC Injector HTC/HTS
accessories or who need to make adjustments to an installed system should
consult "LC Injector HTC/HTS Description and Installation" in Section B.
The Appendices provide useful information such as the Software Flow Chart,
Definition of Terms or the LC Injector HTC/HTS Accessories guide.
NOTE The LC Injector HTC/HTS must be installed and set up properly before the Operating
Instructions in Section A can be used.

13
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Agilent Technologies
2
LC Injector HTC/HTS Operating
Instructions
Using the Control Terminal 15
Menu Screens 16
Function Keys 17
ESCape and STOP Keys 17
Scroll Knob and ENTER Button 17
Methods 18
Creating Methods 18
Edit / View Methods 20
Delete Methods 20
Job and Job Queue 21
Building and Starting a Job Queue 22
Aborting a Job Queue 24
Restarting an Aborted Job Queue 24
Utility Functions 25
Syringe 26
Tray 28
Injector 30
Wash Station 31
Vial 32
Dilutors 33
Tools 34
Logfile 35

14 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
2LC Injector HTC/HTS Operating Instructions
How to Use this Manual
Info Functions 36
Hardware 37
Software 37
Maintenance 38
Free Objects / Free Items 39
Setup Functions 40
Sounds 41
Time 41
Objects 42
This chapter explains the operational parameters of the LC Injector HTC/HTS
System

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 15
LC Injector HTC/HTS Operating Instructions 2
Using the Control Terminal
Using the Control Terminal
The following procedures present the key steps required to set up and process
multiple groups of samples with the LC Injector HTC/HTS. They are intended
to provide an overview for new users and a reminder for infrequent users. The
LC Injector HTC/HTS and all accessories should be installed with Objects
defined correctly. A syringe of the specific type called for by a particular
method should also be installed.
Figure 1 on page 15 illustrates the LC Injector HTC/HTS control terminal and
the conventions used to enter, edit, and view information.
Figure 1 LC Injector HTC/HTS Control Terminal and Conventions
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16 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
2LC Injector HTC/HTS Operating Instructions
Using the Control Terminal
Menu Screens
Different menu screens are displayed, depending on the LC Injector HTC/HTS
operating status and the particular function being accessed by the operator.
All menu screens have the same basic format. The menu title is displayed at
the top of the screen. A list of items is displayed below the title. The date and
time, or status, are shown in the highlighted area above the Function key
labels on the bottom of the screen.
Figure 2 Accessing a Method Screen
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Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 17
LC Injector HTC/HTS Operating Instructions 2
Using the Control Terminal
Function Keys
Options for a particular menu are assigned to the corresponding function keys
(F1, F2, F3, F4) directly below each function key label.
Pressing the function key labeled as Home will always return to the Job Queue
menu.
ESCape and STOP Keys
Press ESCape to return to the previous menu. Press STOP to abort the current
Cycle, Job, or Job Queue.
Scroll Knob and ENTER Button
Rotate the outer knob to scroll through items in a menu list. To select a
highlighted item press the central knob (ENTER). Then use the outer knob to
scroll through available options for that item or to change a numeric value.
Then press the inner knob again to ENTER the displayed option. The inner
knob is also used for other operations that require an ENTER operation to
continue or complete an operation.

18 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
2LC Injector HTC/HTS Operating Instructions
Methods
Methods
Creating Methods
Methods can be defined by the user and assigned names up to eight characters
in length. Methods can be created, copied, edited, and viewed from the
Methods menu. Methods can be viewed (but not edited) from the Job Queue
menus.
Methods are created by either copying an existing Method or creating a new
Method.
To copy a Method, complete the sequence as follows:
1
You will be prompted to enter a name for the new Method.
2Use the scroll knob and the left-right arrow function keys (F2 and F3) to
select among alphanumeric characters and spaces. Press the ENTER function
key (F4) to accept the name.
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8denBZi]dY

Agilent 1290 Infinity LC Injectors HTC/HTS User Manual 19
LC Injector HTC/HTS Operating Instructions 2
Methods
To create a new Method, complete the following steps:
1
2Assign and enter a new Method name as above.
After a copy of the Method has been created, the Method parameters will
display and can be edited. The Cycle and Syringe entries cannot be changed.
3If the Method is new (i.e. added), select and enter a Cycle that is
appropriate for the application.
4Select the specific Syringe to be used by the Method.
5Assign Parameter values according to the application requirements.
Consult “LC-Inj Cycle” on page 180 for details on specific items.
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NOTE Once a Method has been created and saved, the Cycle and Syringe cannot be changed. To
use a different Cycle or Syringe, a new Method must be created.

20 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
2LC Injector HTC/HTS Operating Instructions
Methods
Edit / View Methods
Method parameters (excluding Cycle and Syringe) can be viewed and
changed from the Method menu as follows:
Complete the following menu selections:
1
2Scroll to and select the parameter to be changed. Assign the new value and
press ENTER.
3Exit from Parameter List by pressing either the Home function key (F4) to
return to the top-level Job Queue menu or ESCape to return to the previous
menu.
4Method contents may be viewed from the Job Queue displays by selecting the
desired Job, pressing ENTER, followed by the View Method function key.
Delete Methods
Methods can be deleted from the Methods menu. Methods in use by an active
Job cannot be deleted.
1Complete the following menu selections to delete a Method.
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