Agilent Technologies 8453 User manual

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Temperature Control
Accessory for
Agilent 8453 UV-visible
Spectrophotometer
Operator’s Manual

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
Notices
© Agilent Technologies, Inc. 2000, 2004
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Manual Part Number
89090-90003
Edition
11/2004
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76377 Waldbronn
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Safety Notices
CAUTION
A CAUTION notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
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in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated condi-
tions are fully understood and
met.

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 3
In This Guide…
This handbook describes operation, installation, maintenance and repair,
using GPIB commands, and service of the temperature control accessory.
Part 1 Installing and Maintaining Your Temperature Controller
This part describes how you install, and maintain your temperature control
unit, cell holder and optional external sensor (option #200).
1Description
This chapter contains an overview of the temperature control accessory,
including specifications.
2 Installing Your Controller
This chapter describes how to install your control unit, cell holder, external
sensor and GPIB commands PROM.
3 Simple Maintenance
This chapter describes maintenance tasks you can do on your control unit and
cell holder.
Part 2 Using Your Temperature Controller
This part describes how you operate your temperature controller; that is the
temperature control unit with the cell holder and external sensor (option
#200). There is also a description of what you do in response to the messages
that the control unit displays.
4 Operating Your Control Unit
This chapter describes how to prepare your control unit for operation.
5 Display Summary
This chapter contains a summary of the different control and read-out
functions.

4 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
6 Using External Sensor
This chapter describes how to get more accurate temperature monitoring of
your sample.
7Messages
This chapter contains the messages that the control unit displays.
Part 3 Using GPIB Commands with the Temperature Controller
This part is intended to provide information necessary to interface the
temperature control unit to various computer systems, for option #305. It is
written from a software interfacing point of view and assumes that the reader
is knowledgeable in software interfacing concepts.
8 Communicating GPIB Commands
This chapter contains information about controlling and communicating with
the temperature control unit.
9 GPIB Commands
This chapter contains information about controlling and communicating with
the temperature control unit.
Part 4 Servicing the Temperature Controller
This part is intended only for use by Agilent Technologies service personnel or
other qualified engineers. It contains a description of the electronics, and
information about the service, cables and connectors, and replacement parts
of your temperature controller.
10 Electronics of the Temperature Control Accessory
This chapter describes the GPIB commands for controlling the temperature
control unit.
11 Cables and Connectors
This chapter contains information about cables used with the temperature
controller.

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 5
12 Troubleshooting
This chapter contains troubleshooting instructions for trained personnel.
13 Identifying and Replacing Parts
This chapter describes how to find parts information.
A Safety Information
This chapter contains relevant safety information.

6 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 7
Contents
1Description13
Temperature Control Unit 14
Temperature-Controlled Cell Holder 15
External Sensor 16
GPIB Commands 16
2 Installing Your Controller 17
Choosing a Suitable Place 18
Unpacking Your Controller 19
Unpacking the Controller Options 20
Setting Up Your Control Unit 21
Viewing the Control Unit 22
Selecting Voltage 23
Selecting Fuse 24
Connecting Cables 26
Setting Up the Temperature-Controlled Cell Holder 27
Removing the Multicell Transport Cell Holder 28
Removing other Cell Holders 28
Viewing the Cell Holder 28
Installing the Temperature-Controlled Cell Holder 29
Connecting Cables 29
Connecting Flow Cell and Heat Exchanger 30
Setting Up Your External Sensor 32
Viewing the External Sensor 33
Connecting Cables 33

8 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer
Contents
3 Simple Maintenance 35
Changing Fuse 37
Line (Primary) Fuse 37
Internal (Secondary) Fuse 37
Leaking Fittings on Heat Exchanger 42
Stage 1: Disassembling Heat Exchanger 43
Stage 2: Changing Ferrule on Fittings 43
Stage 3: Changing Fitting 43
Stage 4: Reassembling Heat Exchanger 45
Cleaning Cells 46
Removing Cell 46
Replacing Cell 48
Cleaning Heat-Exchanger Tubing 49
4 Operating Your Control Unit 51
Before you Start 52
Turning on Line Power 53
Checking Operation 54
Turning off Line Power 55
Using Functions 56
Selecting Functions 56
Setting Functions 57
Resetting Functions 58
Setting Temperature 59
Example for Setting Temperature 59
Example for Correcting a Wrong Set Temperature 60
Changing Brightness 61
Changing Units 62
Turning off Peltier Element 64

Contents
Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 9
Selecting Stirring 64
Setting System Functions 65
Setting Remote Mode 65
Changing GPIB Address 66
Starting Self-Test Manually 67
Hints for Operation 68
5 Display Summary 71
Normal Mode 72
Edit Mode 72
6 Using External Sensor 73
7Messages77
Responding to Messages 78
OVERLOAD! 78
WARNING 1 78
WARNING 2 80
Display Messages 81
OUT OF RANGE 81
Status Messages 82
Status Lamps 83
Ready 83
Error 83
Stirrer 83
8 Communicating GPIB Commands 85
Communication Interface 86
Remote Mode 88
GPIB Control 89

10 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer
Contents
Service Request 90
Trigger Lines 90
Device Clear 91
9 GPIB Commands 93
Buffers 94
Instruction Format 95
General Description 95
Detailed Description 96
CSM – Agilent ChemStation Mode 98
ERR – Error 100
EXT – External Temperature 104
IDY – Identify 105
MSK – Service Request Mask 106
PEL – Peltier 108
REM – Remote 109
SET – Set Cell Temperature 110
SEU – Select Unit 111
SPE – Speed 112
STA – Status 113
STR – Stirrer 115
TEM – Cell Temperature 116
TRA – Trigger Acknowledge 117
TRG – Trigger Out 118
TST – Test 119