Agilent Technologies 8453 User manual

A
Temperature Control
Accessory for
Agilent 8453 UV-visible
Spectrophotometer
Operator’s Manual

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
Notices
© Agilent Technologies, Inc. 2000, 2004
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tronic storage and retrieval or translation
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ment and written consent from Agilent
Technologies, Inc. as governed by United
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Manual Part Number
89090-90003
Edition
11/2004
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76377 Waldbronn
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ard. It calls attention to an operat-
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A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
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in personal injury or death. Do not
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notice until the indicated condi-
tions are fully understood and
met.

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 3
In This Guide…
This handbook describes operation, installation, maintenance and repair,
using GPIB commands, and service of the temperature control accessory.
Part 1 Installing and Maintaining Your Temperature Controller
This part describes how you install, and maintain your temperature control
unit, cell holder and optional external sensor (option #200).
1Description
This chapter contains an overview of the temperature control accessory,
including specifications.
2 Installing Your Controller
This chapter describes how to install your control unit, cell holder, external
sensor and GPIB commands PROM.
3 Simple Maintenance
This chapter describes maintenance tasks you can do on your control unit and
cell holder.
Part 2 Using Your Temperature Controller
This part describes how you operate your temperature controller; that is the
temperature control unit with the cell holder and external sensor (option
#200). There is also a description of what you do in response to the messages
that the control unit displays.
4 Operating Your Control Unit
This chapter describes how to prepare your control unit for operation.
5 Display Summary
This chapter contains a summary of the different control and read-out
functions.

4 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
6 Using External Sensor
This chapter describes how to get more accurate temperature monitoring of
your sample.
7Messages
This chapter contains the messages that the control unit displays.
Part 3 Using GPIB Commands with the Temperature Controller
This part is intended to provide information necessary to interface the
temperature control unit to various computer systems, for option #305. It is
written from a software interfacing point of view and assumes that the reader
is knowledgeable in software interfacing concepts.
8 Communicating GPIB Commands
This chapter contains information about controlling and communicating with
the temperature control unit.
9 GPIB Commands
This chapter contains information about controlling and communicating with
the temperature control unit.
Part 4 Servicing the Temperature Controller
This part is intended only for use by Agilent Technologies service personnel or
other qualified engineers. It contains a description of the electronics, and
information about the service, cables and connectors, and replacement parts
of your temperature controller.
10 Electronics of the Temperature Control Accessory
This chapter describes the GPIB commands for controlling the temperature
control unit.
11 Cables and Connectors
This chapter contains information about cables used with the temperature
controller.

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 5
12 Troubleshooting
This chapter contains troubleshooting instructions for trained personnel.
13 Identifying and Replacing Parts
This chapter describes how to find parts information.
A Safety Information
This chapter contains relevant safety information.

6 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 7
Contents
1Description13
Temperature Control Unit 14
Temperature-Controlled Cell Holder 15
External Sensor 16
GPIB Commands 16
2 Installing Your Controller 17
Choosing a Suitable Place 18
Unpacking Your Controller 19
Unpacking the Controller Options 20
Setting Up Your Control Unit 21
Viewing the Control Unit 22
Selecting Voltage 23
Selecting Fuse 24
Connecting Cables 26
Setting Up the Temperature-Controlled Cell Holder 27
Removing the Multicell Transport Cell Holder 28
Removing other Cell Holders 28
Viewing the Cell Holder 28
Installing the Temperature-Controlled Cell Holder 29
Connecting Cables 29
Connecting Flow Cell and Heat Exchanger 30
Setting Up Your External Sensor 32
Viewing the External Sensor 33
Connecting Cables 33

8 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer
Contents
3 Simple Maintenance 35
Changing Fuse 37
Line (Primary) Fuse 37
Internal (Secondary) Fuse 37
Leaking Fittings on Heat Exchanger 42
Stage 1: Disassembling Heat Exchanger 43
Stage 2: Changing Ferrule on Fittings 43
Stage 3: Changing Fitting 43
Stage 4: Reassembling Heat Exchanger 45
Cleaning Cells 46
Removing Cell 46
Replacing Cell 48
Cleaning Heat-Exchanger Tubing 49
4 Operating Your Control Unit 51
Before you Start 52
Turning on Line Power 53
Checking Operation 54
Turning off Line Power 55
Using Functions 56
Selecting Functions 56
Setting Functions 57
Resetting Functions 58
Setting Temperature 59
Example for Setting Temperature 59
Example for Correcting a Wrong Set Temperature 60
Changing Brightness 61
Changing Units 62
Turning off Peltier Element 64

Contents
Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 9
Selecting Stirring 64
Setting System Functions 65
Setting Remote Mode 65
Changing GPIB Address 66
Starting Self-Test Manually 67
Hints for Operation 68
5 Display Summary 71
Normal Mode 72
Edit Mode 72
6 Using External Sensor 73
7Messages77
Responding to Messages 78
OVERLOAD! 78
WARNING 1 78
WARNING 2 80
Display Messages 81
OUT OF RANGE 81
Status Messages 82
Status Lamps 83
Ready 83
Error 83
Stirrer 83
8 Communicating GPIB Commands 85
Communication Interface 86
Remote Mode 88
GPIB Control 89

10 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer
Contents
Service Request 90
Trigger Lines 90
Device Clear 91
9 GPIB Commands 93
Buffers 94
Instruction Format 95
General Description 95
Detailed Description 96
CSM – Agilent ChemStation Mode 98
ERR – Error 100
EXT – External Temperature 104
IDY – Identify 105
MSK – Service Request Mask 106
PEL – Peltier 108
REM – Remote 109
SET – Set Cell Temperature 110
SEU – Select Unit 111
SPE – Speed 112
STA – Status 113
STR – Stirrer 115
TEM – Cell Temperature 116
TRA – Trigger Acknowledge 117
TRG – Trigger Out 118
TST – Test 119

Contents
Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 11
10 Electronics of the Temperature Control Accessory 121
Electronics Overview 122
Power Supply 124
Line Fuses 124
Internal Fuses 125
Transformer, Regulation 125
Power Fail Detector 125
Power On Detector 125
GND, AGND, PGND, SGND, Shield 126
Microcontroller and Peripherals 127
PROM Card 128
Latch, Remote Control Connector 128
Lines to Power Amplifiers 128
Lines to Sensor Electronic 129
EEPROM 129
Stirrer Driver, Stirrer 129
Reset, NMI 129
Keyboard Display Electronic 130
Keyboard 130
LEDs 131
Control Logic 131
8-Character Display 131
Power Amplifier 132
Switch Controller, Current Sensor 132
Switch 133
H-Bridge, Peltier Element 133
Sensor Electronic 134
Current Source 135
Multiplexer, Demultiplexer 135
Measuring Principle 136
+5 V Regulator, Voltage Divider 137

12 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer
Contents
Analog to Digital Converter (ADC) 137
11 Cables and Connectors 139
Cables 140
Connectors 142
12 Troubleshooting 147
General Description 148
Diagnostic During Operation and Power-On 148
Starting Self-Test Manually 148
Basic Failures 150
Error Messages 152
13 Identifying and Replacing Parts 163
Parts Identification 165
Changing Parts 167
Changing Temperature Controller Board 167
Changing Inner Cabinet Assembly 168
Changing Cell Holder Cable 169
A Safety Information 171
Safety Information 172
General 172
Operation 172
Safety Symbols 173
Radio Interference 176
Manufacturer’s Declaration 176
Herstellerbescheinigung 176
Agilent Technologies on Internet 177
Index 179

13
Temperature Control Accessory for Agilent 8453 UV-visible
Spectrophotometer
Operator’s Manual
Agilent Technologies
1
Description
Temperature Control Unit 14
Temperature-Controlled Cell Holder 15
External Sensor 16
GPIB Commands 16
The temperature control accessory consists of the control unit and
temperature-controlled cell holder for the Agilent 8453 UV-visible
spectrophotometer for sample temperature control. The digitally controlled
unit uses thermoelectric heating and cooling (Peltier principle) over a 60 °
temperature range, 10 °C – 70 °C at 20 °C. The temperature control accessory
gives a stable temperature for a wide range of applications, for example,
enzyme kinetic rate determinations and equilibrium studies.
The Peltier temperature control accessory consists of four parts.
•temperature control unit
•temperature-controlled cell holder
•external sensor (optional)
•GPIB commands

14 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
1Description
Temperature Control Unit
Temperature Control Unit
The control unit gives sample temperature control, sample stirring, and status
and error indication. It controls the temperature of the cell holder and is
operated manually through the keyboard interface or remotely through GPIB
commands.
NOTE The below temperature range describes the principle controller features of the temperature
controll unit. The cell holder device connected defines the applicable temperature settings
Table 1 Control Unit Technical Specifications
Power supply 100–120 VAC, 50–60 Hz
220–240 VAC, 50–60 Hz
Temperature set range -10 °C–120 °C *
* or equivalent in K or °F
Temperature set resolution 0.1
Temperature Display Range -10 °C–120 °C †
† or equivalent in K or °F
Display resolution 0.1
Electromagnetic stirrer speed range 40–1000 rpm

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 15
Description 1
Temperature-Controlled Cell Holder
Temperature-Controlled Cell Holder
The cell holder is a temperature-controlled cell holder for 1 cm sample cells
(cuvettes) and flow cells.
Table 2 Cell Holder Technical Specifications
Operating temperature range 10 °C–70 °C at 20 °C ambient temperature
Accessible temperature range 10 °C below ambient temperature
Temperature stability 50 °C above ambient temperature
10 °C–60 °C ±0.1K
>60 °C, ±0.2K
Temperature accuracy 0 °C–<20 °C, ±0.3K
20 °C–40 °C, ±0.2K
>40 °C–60 °C, ±0.3 K
Temperature Reproducibility 0 °C–60 °C, ±0.1 K
>60 °C, ±0.2K
Typical Step Response 5.5 °C/min heating
3.0 °C/min cooling

16 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
1Description
External Sensor
External Sensor
The external sensor is an optional temperature sensing probe. The
temperature probe measures the actual temperature of your sample. It gives
the possibility of more accurate temperature control, when used with the cell
holder and control unit.
GPIB Commands
The GPIB commands are available to remotely control the temperature control
unit via a GPIB connection by means of GPIB commands.

17
Temperature Control Accessory for Agilent 8453 UV-visible
Spectrophotometer
Operator’s Manual
Agilent Technologies
2
Installing Your Controller
Choosing a Suitable Place 18
Unpacking Your Controller 19
Setting Up Your Control Unit 21
Setting Up the Temperature-Controlled Cell Holder 27
Connecting Flow Cell and Heat Exchanger 30
Setting Up Your External Sensor 32

18 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
2 Installing Your Controller
Choosing a Suitable Place
Choosing a Suitable Place
Check carefully before you begin that the place you choose to install your
control unit meets the requirements shown in Table 3.
Table 3 Choosing a Suitable Place
Dimensions 325 × 105 × 285 mm
(12.8 × 4.1 × 11.2 inches)
(width × height × depth)
Leave adequate space, at least 80 mm (3 inches), at rear for
access to connectors.
Weight 5.5 kg (11.8 lb)
Environment Operating temperature 0–55 °C (32–131°F)
Humidity <95% (non-condensing)
Line power 100–120 VAC ±10%
220–240 VAC ±10%
Line frequency 48–66 Hz
Power consumption 90 VA maximum
NOTE The Agilent 8453, the HP 8452A or HP 8451A spectrophotometers must be within 30 cm.

Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual 19
Installing Your Controller 2
Unpacking Your Controller
Unpacking Your Controller
Inspect carton. If you find signs of external damage, contact your local Agilent
Technologies office.
Open top of carton. Lift out accessory box. Check your accessories against the
list below.
Table 4 Controller Accessories
Description Part Number Check
Power cord as ordered . . .
Handbook 89090-90003 . . .
Hexagonal balldriver 8710-1900 . . .
Primary fuses:
T0.5 Amp fuse 2110-0202 . . .
T1.0 Amp fuse 2110-0007 . . .
Secondary fuses:
3.0 Amp fuse 2110-0003 . . .
0.5 Amp fuse 2110-0012 . . .
Tubing for heat exchanger 5041-2189

20 Temperature Control Accessory for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual
2 Installing Your Controller
Unpacking Your Controller
Unpacking the Controller Options
If you have ordered the options for use with the temperature controller:
Check the options against the list below.
Table 5 Controller Options
Description Option Check
External Sensor #200 . . .