Agilent Technologies 7100 User manual

Agilent Technologies
Agilent 7100
Capillary Electrophoresis
System
User Manual

Agilent 7100 Capillary Electrophoresis System
Notices
© Agilent Technologies, Inc. 2009
No part of this manual may be reproduced in
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tronic storage and retrieval or translation
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Manual Part Number
G7100-90000
Edition
12/2009
Printed in Germany
Agilent Technologies, Deutschland GmbH
Hewlett-Packard-Strasse 8
76337 Waldbronn
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Safety Notices
CAUTION
A CAUTION notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
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in personal injury or death. Do not
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notice until the indicated condi-
tions are fully understood and
met.
Research Use Only

Agilent 7100 Capillary Electrophoresis System User Manual 3
In This Guide…
This guide contains information to install and operate the
Agilent 7100 Capillary Electrophoresis System.
1 Introduction
This chapter gives an introduction to the instrument,
instrument overview and internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental
requirements, physical and performance specifications.
3 Installing the Instrument
In this chapter you will find information about how to unpack
and to set up the Agilent CE and information about the
configuration of Instrument.
4 LAN Configuration
This chapter provides information on connecting the CE
instrument to the Agilent ChemStation PC.
5 Using the Agilent 7100 Capillary Electrophoresis System
This chapter provides information on how to set up the CE
instrument for an analysis and explains the basic settings.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and
diagnostic features and the different user interfaces.
7 Hardware Information
This chapter describes the instrument in more detail on
hardware and electronics.
8 Maintenance
This chapter describes the maintenance of the CE instrument.

4 Agilent 7100 Capillary Electrophoresis System User Manual
9 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
10 Appendix
This chapter provides additional information on safety, legal
and web content associated with your 7100 CE instrument.

Agilent 7100 Capillary Electrophoresis System User Manual 5
Contents
1 Introduction 13
Introduction to the Agilent 7100 Capillary Electrophoresis System 14
Getting Help 14
Where to Find Information 15
What Learning Products are Available? 16
2 Site Requirements and Specifications 17
Site Requirements 18
Power Consideration 18
Power Cords 19
Bench Space 20
Environment 20
Physical Specifications 21
Performance Specifications 22
Anforderungen an den Aufstellungsort 23
Stromversorgung 23
Netzkabel 24
Platzbedarf 25
Umgebungsbedingungen 25
Abmessungen und Gewicht 26
Leistungsspezifikationen 27
Exigences d’installation 28
Remarques à propos de l’alimentation 28
Câbles d’alimentation 29
Encombrement 30
Environnement 30
Caractéristiques physiques 31
Caractéristiques de performance 32

6 Agilent 7100 Capillary Electrophoresis System User Manual
Contents
Requisiti ambientali 33
Alimentazione 33
Cavi di alimentazione 34
Spazio necessario 35
Ambiente 35
Specifiche fisiche 36
Specifiche delle prestazioni 37
Requisitos de las instalaciones 38
Consideraciones sobre la corriente 38
Cables de alimentación 39
Espacio en el banco 40
Entorno 40
Especificaciones físicas 41
Especificaciones de rendimiento 42
設置について 43
電源について 43
電源コード 44
作業台スペース 45
環境条件 45
物理的仕様 46
性能仕様 47
场地要求 48
电源要求 48
电源线 49
工作台位置 50
环境 50
物理规格 51
性能规格 52
3 Installing the Instrument 53
Unpacking the Agilent CE Instrument 54
Damaged Packaging 54
Unpacking the CE Instrument 54

Contents
Agilent 7100 Capillary Electrophoresis System User Manual 7
Delivery Checklist 55
Installing the Instrument 57
Physical Connection 57
4 LAN Configuration 61
What You Have to Do First 62
TCP/IP Parameter Configuration 63
Configuration Switch 64
Initialization Mode Selection 65
Link Configuration Selection 69
Storing the Settings Permanently with Bootp 70
Manual Configuration 71
With Telnet 72
Agilent Bootp Service 75
Location of the Media Access Control (MAC) Address 76
5 Using the Agilent 7100 Capillary Electrophoresis System 77
System Definition 78
Controlling the Agilent CE Instrument 79
Before You Start the Agilent CE Instrument 79
Starting Up the Agilent CE Instrument 79
Starting Your Computer and the Agilent ChemStation Software 81
Starting the Agilent ChemStation Software the First Time 82
Working with the CE Diagram 84
Common Software Toolbar 84
Software Toolbar 85
Graphical User Interface Explanation 86
Menus in the CE and DAD Diagram 88
System Vialtable 90
How to Prepare your Agilent CE Instrument for Analysis 91
Capillary Electrophoresis, CE 92
How to Use Your Agilent CE Instrument for Capillary Electrophoresis
Separations 92

8 Agilent 7100 Capillary Electrophoresis System User Manual
Contents
Agilent CE Method Parameters 92
Vials 96
Cassette Temperature 97
High Voltage System 98
Posttime and Stoptime 100
Replenishment 100
Preconditioning 101
Injection 103
Timetable 105
Detector Parameters 107
Switching the Lamp On 110
Preparing the Replenishment System if Needed 111
Preparing Vials for Buffer and Sample 115
Installing a Capillary in the Alignment Interface 124
Installing a Capillary in an Empty Capillary Cassette 128
Inserting the Cassette 132
Fraction Collection 135
Capillary Electrophoresis, Plus High Pressure CE+p 136
Requirements 136
Outlet Lift Connection to the Tubing System 138
Preparing the Agilent CE Instrument for High Pressure Use 140
Operation and Function in CE+p Mode 143
Injection Options with CE+p 146
Timetable Options with CE+p 146
CE+p Control Using the CE Diagram 147
Capillary Electrochromatography, CEC 148
How to Use Your Agilent CE Instrument for Capillary
Electrochromatography 148
Preparing the Agilent CE Instrument for High Pressure Use 150
CEC-Specific GUI Explanation 153
Injection Options with CEC 156
Timetable Options with CEC 157
CEC Control Using the CE Diagram 158

Contents
Agilent 7100 Capillary Electrophoresis System User Manual 9
Running the CEC Analysis 159
6 Troubleshooting and Diagnostics 161
CE Problem Solving 162
Power On Fail 162
Leaks 163
Blocked Replenishment Needle 165
Leakage Current 165
Broken Capillary 166
Contaminated Insulation Plate 166
Empty Injection Vial 167
Dirt in the Vials 167
Air Bubble in Injection Vial or Run Buffer Vials 167
Air Bubble in Capillary 167
Problems with the Replenishment System and Buffers
Containing SDS 168
Agilent Lab Advisor Software 169
Configuration 169
7 Hardware Information 171
Identifying Instrument Components 172
7100 Capillary Electrophoresis Instrument Electronics 173
Firmware 176
Resident System 176
Main System 176
Firmware Updates 177
Diode Array Detector 179
DAD Control 180
Digital to Analog Converter 181
Changing the Fuses for the Power Supplies 182
Installing Drainage Tubing 183
External Water Bath for Tray Cooling (Optional) 184
Analog to Digital Converter 186

10 Agilent 7100 Capillary Electrophoresis System User Manual
Contents
8 Maintenance 189
Standard Operating Procedures for Maintenance of Your
Agilent CE Instrument 190
Overview of Maintenance 191
Early Maintenance Feedback (EMF) 194
EMF Counters 194
Using the EMF Counters 194
Setting the EMF Limits 195
Cleaning the Electrodes, Pre-punchers and Insulation Plate 196
Prepare the Agilent CE Instrument 197
Remove the Covers 198
Accessing the Electrodes 199
Accessing the Liquid Handling Module 201
Accessing and Removing the Pre-punchers 203
Cleaning the Electrodes 205
Cleaning the Pre-punchers 206
Cleaning the Insulation Plate 207
Reinstalling the Pre-punchers 208
Reinstalling the Electrodes and the Insulation Plate 210
Reinstall the Capillary Cassette 215
Reinstall the Front Cover 215
Maintenance of the Replenishment System 216
Changing the Buffer Composition in the Replenishment System 217
The Replenishment System Will Not Be Used for Some Time 218
Further Action 219
Operations for Replenishment Maintenance 219
Changing the Air Inlet Filter 222
Accessing the Air Filter 222
Changing the Lamp 224
Removing the Existing Lamp 225
Inserting a New Lamp 227
Further Action 227
Cleaning the Instrument 228

Contents
Agilent 7100 Capillary Electrophoresis System User Manual 11
9 Parts and Materials for Maintenance 229
Overview of Maintenance Parts 230
10 Appendix 231
General Safety Information 232
Operation 232
Allgemeine Sicherheitsinformation 235
Informations générales de sécurité 238
Informazioni generali sulla sicurezza 241
Información de seguridad 244
安全に関する一般的な情報 247
一般安全信息 250
Setting Up a Test Sample Run 252
Method Parameter CE (Default Settings) 253
Method Parameter DAD 255
Chemical and Biological Safety 256
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 257
Radio Interference 258
Sound Emission 259
Legal Notice 260
Agilent Technologies on Internet 261
Index 263

12 Agilent 7100 Capillary Electrophoresis System User Manual
Contents

13
Agilent 7100 Capillary Electrophoresis System
User Manual
Agilent Technologies
1
Introduction
Introduction to the Agilent 7100 Capillary Electrophoresis System 14
Where to Find Information 15
What Learning Products are Available? 16
This chapter gives an introduction to the Agilent 7100 Capillary
Electrophoresis System and where to find specific information or help.

14 Agilent 7100 Capillary Electrophoresis System User Manual
1 Introduction
Introduction to the Agilent 7100 Capillary Electrophoresis System
Introduction to the Agilent 7100 Capillary Electrophoresis
System
This handbook gives an overview of your Agilent 7100 Capillary
Electrophoresis System. It is designed to get you started working with the
system. We strongly advise you to make extensive use of the online help when
working with the system. There you find detailed reference and task
information which complements the overview given in this guide. Refer to
“Getting Help” later in this section for information on using the online help of
the ChemStation. Refer to “Where to Find Information” for help in finding
information on certain tasks.
Getting Help
You can get help by choosing the Help item from the top menu. This puts you
into the help index. From there you can browse through the information by
selecting the appropriate jumps (underlined words). To access specific
information in the help:
When you are in the Agilent ChemStation or Lab Advisor software:
•select a context-sensitive element and press F1.
When you are in the online help:
•search for a specific keyword using the search command,
•select a jump - a jump is an underlined word you can select to go to a topic
related to the word, or
•select a word or phrase with a dotted underline to see its definition. The
online help also contains online information on how to use help.

Agilent 7100 Capillary Electrophoresis System User Manual 15
Introduction 1
Where to Find Information
Where to Find Information
Table 1 Where to find information
Task Refer to
•Installation of the Agilent CE System,
installing the external water bath,
installing the high sensitivity cell
"The Core Agilent CE Instrument" “Installing the
Instrument” on page 53
•Setting up the Agilent CE System for
CE analysis, CE+p
Chapter “Using the Agilent 7100 Capillary
Electrophoresis System” on page 77"
•How to use the Agilent CE System in
the CE+p mode
“Capillary Electrophoresis, Plus High Pressure
CE+p” on page 136
•How to use the Agilent CE System for
capillary electrochromatography
“Capillary Electrochromatography, CEC” on page 148
•How to maintain the Agilent CE
System
Chapter “Maintenance” on page 189
•Setting up a sequence Online help "Understanding Your ChemStation"
•Data analysis Online help "Understanding Your ChemStation"
•Setting up a report Online help "Understanding Your ChemStation"
•Spectral library search “ChemStation Applications” handbook and online help
•Setting up customized reports “ChemStation Applications” handbook
•Safety information Safety information in “Appendix” on page 231
•Learning ChemStation concepts “Understanding Your ChemStation” handbook
•Learning the theory of the technique High Performance Capillary Electrophoresis: An
Introduction”
The CE Partner CD-ROM: “The comprehensive,
interactive tool for beginners and advanced users of
CE”
•Application Agilent web page (http://www.agilent.com), search for
Application notes

16 Agilent 7100 Capillary Electrophoresis System User Manual
1 Introduction
What Learning Products are Available?
What Learning Products are Available?
The Agilent CE System is supplied with the following:
•User's Guide
•High Performance Capillary Electrophoresis: An Introduction
The Agilent ChemStation and Lab Advisor/Instrument Utilities software DVD
is supplied with the following electronic documents:
•Installing Your Agilent ChemStation for LC and CE Systems
•Installing and Understanding Your Spectra Module
•Understanding Your Agilent ChemStation
•Online help
•Understanding Your Agilent ChemStation for CE Systems

17
Agilent 7100 Capillary Electrophoresis System
User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 18
Physical Specifications 21
Performance Specifications 22
Anforderungen an den Aufstellungsort 23
Abmessungen und Gewicht 26
Leistungsspezifikationen 27
Exigences d’installation 28
Caractéristiques physiques 31
Caractéristiques de performance 32
Requisiti ambientali 33
Specifiche fisiche 36
Specifiche delle prestazioni 37
Requisitos de las instalaciones 38
Especificaciones físicas 41
Especificaciones de rendimiento 42
設置について 43
物理的仕様 46
性能仕様 47
场地要求 48
物理规格 51
性能规格 52
This chapter provides information on the environmental requirements, as well
as physical and performance specifications.

18 Agilent 7100 Capillary Electrophoresis System User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
7100 Capillary Electrophoresis instrument.
Before you begin installation, check carefully that the place you have chosen
meets the requirements below.
Power Consideration
The instrument power supply has wide ranging capabilities and accepts any
standard line voltage in the range mentioned in Table 2 on page 21.
Consequently, there is no voltage selector on the back of the detector.
WARNING
The module is partially energized when switched off, as long as the power cord is
plugged in.
Repair or maintenance work on the module can lead to personal injuries, for
example a shock hazard, when the cover is opened and the module is connected to
the power.
•Make sure that it is always possible to access the power plug.
•Remove the power cable from the instrument before opening the cover.
•Do not connect the power cable to the instrument while the electronic box in the
back of the instrument is opened or side panels are removed.
WARNING
Incorrect line voltage on the instrument
Shock hazard or damage to your instrument can result if the device is connected to a
line voltage that is higher than specified.
•Connect your instrument to the specified line voltage.

Agilent 7100 Capillary Electrophoresis System User Manual 19
Site Requirements and Specifications 2
Site Requirements
Power Cords
Different power cords are delivered with the instrument. The female end of all
power cords is identical. It plugs into the power-input socket at the rear. The
male end of each power cord is different and designed to match the wall socket
of a particular country or region.
CAUTION
Inaccessible power plug.
In the case of an emergency it must be possible to disconnect the instrument from the
power line at any time.
•Make sure the power connector of the instrument can be easily reached and
unplugged.
•Provide sufficient space behind the power socket of the instrument to unplug the
cable.
WARNING
Absence of a ground connection or use of an unspecified power cord
The absence of a ground connection or the use of an unspecified power cord can
lead to an electric shock or short circuit.
•Never operate your instrument from a power outlet without a ground connection.
•Never use a power cord other than the Agilent Technologies power cord designed
for your region.
WARNING
Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
•Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

20 Agilent 7100 Capillary Electrophoresis System User Manual
2 Site Requirements and Specifications
Site Requirements
Bench Space
The instrument dimensions and weight mean that the instrument can be
placed on almost any desk or laboratory bench. It needs an additional 2.5 cm
(1.0 inch) of space on either side and approximately 8 cm (3.1 inches) in the
rear for air circulation and electric connections.
If the Agilent 7100 Capillary Electrophoresis instrument is to be placed on a
bench, make sure that the bench is designed to bear the weight of the system.
The instrument must be operated in a upright position.
Environment
Your instrument will work within the specifications for ambient temperatures
and relative humidity described in Table 2 on page 21.
CAUTION
Condensation within the module
Condensation will damage the system electronics.
•Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
•If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.