Agilent Technologies Bravo Setup guide


Bravo Automated
Liquid-Handling
Platform
Safety and Installation
Guide
Original Instructions

Notices
© Agilent Technologies, Inc. 2011
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or
translation into a foreign language) without
prior agreement and written consent from
Agilent Technologies, Inc. as governed by
United States and international copyright
laws.
User Guide Part Number
G5409-90007
Edition
Revision 00, June 2011
Contact Information
Agilent Technologies Inc.
Automation Solutions
5301 Stevens Creek Blvd.
Santa Clara, CA 95051
USA
Technical Support: 1.800.979.4811
or +1.408.345.8011
[email protected]om
Customer Service: 1.866.428.9811
or +1.408.345.8356
customerservice.auto[email protected]
European Service: [phone]
[e-mail]
Documentation feedback:
[email protected]
Web:
www.agilent.com/lifesciences/
automation
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ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
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contained herein, including but not
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The hardware and/or software described in
this document are furnished under a
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Restricted Rights Legend
If software is for use in the performance of
a U.S. Government prime contract or sub-
contract, Software is delivered and
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ware” as defined in DFAR 252.227-7014
(June 1995), or as a “commercial item” as
defined in FAR 2.101(a) or as “Restricted
computer software” as defined in FAR
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agency regulation or contract clause. Use,
duplication or disclosure of Software is
subject to Agilent Technologies’ standard
commercial license terms, and non-DOD
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ernment will receive no greater than
Restricted Rights as defined in FAR 52.227-
19(c)(1-2) (June 1987). U.S. Government
users will receive no greater than Limited
Rights as defined in FAR 52.227-14
(June1987) or DFAR 252.227-7015 (b)(2)
(November 1995), as applicable in any
technical data.
Safety Notices
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
A CAUTION notice denotes a hazard. It
calls attention to an operating procedure,
practice, or the like that, if not correctly
performed or adhered to, could result in
damage to the product or loss of important
data. Do not proceed beyond a CAUTION
notice until the indicated conditions are
fully understood and met.

iiiBravo Automated Liquid-Handling Platform User Guide
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
About this guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi
Reporting problems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
1. Safety guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
General safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Safety and regulatory compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Emergency stops. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Mechanical hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Electrical hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Chemicals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Ergonomics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Laser hazard. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2. Installing the Bravo Platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Workflow for installing the Bravo Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Laboratory setup requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Connecting the Bravo Platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Installing the pipette head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Installing the Light Curtain and front and side shields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Installing the rear shield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Contents
iv Bravo Automated Liquid-Handling Platform User Guide

v
Bravo Automated Liquid-Handling Platform
Safety and Installation Guide

Preface
This preface contains the following topics:
•“About this guide” on page vi
•“Reporting problems” on page vii

About this guide
vi Bravo Automated Liquid-Handling Platform User Guide
About this guide
Who should read this guide
This user guide is for people with the following job roles:
What this guide covers
This guide describes the following:
•Potential safety hazards of the Bravo Platform and how to avoid them.
•Installation instructions, including specifications and site requirements for
the Bravo Platform.
Related guides
The Bravo Automated Liquid Handling Platform Safety and Installation
Guide should be used in conjunction with the following user documents:
•Bravo Automated Liquid Handling Platform User Guide. Explains how to
set up and operate the Bravo Platform and accessories.
•VWorks Automation Control Setup Guide. Explains how to define labware
and labware classes, liquid classes, and pipetting techniques, and how to
track and manage labware in storage.
•VWorks Automation Control User Guide. Explains how to add devices,
create protocols, and set task parameters for each device in the system.
•VWorks Software Quick Start. Provides an overview of how to use the
VWorks Automation Control software.
•Third- party device user documents. Explain how to set up and use the
third- party devices.
For unpacking instructions, see the Bravo Platform Unpacking Guide.
Job role Responsibilities
Installer Unpacks, installs, and tests the Bravo Platform before it is
used.
Integrator Configures hardware and writes software.
Lab manager,
administrator,
or technician
•Manages the automation system that contains the Bravo
Platform
•Develops the applications that are run on the system
•Develops training materials and standard operating
procedures for operators
Operator Performs the daily production work on the Bravo Platform
and solves routine problems.

vii
Reporting problems
Bravo Automated Liquid-Handling Platform User Guide
Accessing Agilent Technologies Automation Solutions user guides
You can search the online knowledge base or download the latest version of
any PDF file from the Agilent Technologies web site at www.agilent.com/
lifesciences/automation.
Safety information for the devices appears in the corresponding device user
guide. You can also search the knowledge base or the PDF files for safety
information.
Related topics
Reporting problems
Contacting Automation Solutions Technical Support
If you find a problem with the Bravo Platform, contact Automation Solutions
Technical Support at one of the following:
Europe
Phone: [phone]
email: [e-mail]
US and rest of world
Phone: [phone] (US only) or +1.408.345.8011
email: [email protected]
Reporting hardware problems
When contacting Agilent Technologies, make sure you have the serial number
of the device ready. You can find the serial number on the Bravo Platform
serial number label.
Reporting software problems
When you contact Automation Solutions Technical Support, make sure you
provide the following:
•Short description of the problem
•Relevant software version number (for example, automation control
software, diagnostics software, ActiveX control software, and firmware)
For information about... See...
Reporting problems “Reporting problems” on page vii
Safety precautions “Safety guidelines” on page 1
How to install the Bravo Platform “Installing the Bravo Platform” on
page 17

Reporting problems
viii Bravo Automated Liquid-Handling Platform User Guide
•Error message text (or screen capture of the error message dialog box)
•Relevant files, such as log files
Reporting user guide problems
If you find a problem with this user guide or have suggestions for
improvement, send your comments in an email to
documentation.aut[email protected].
Related topics
For information about... See...
What this guide covers “About this guide” on page vi
Safety precautions “Safety guidelines” on page 1
How to install the Bravo
Platform
“Installing the Bravo Platform” on
page 17

1
Bravo Automated Liquid-Handling Platform
Safety and Installation Guide

1
Safety guidelines
This chapter contains the following topics:
•“General safety information” on page 2
•“Safety and regulatory compliance” on page 5
•“Emergency stops” on page 7
•“Mechanical hazards” on page 8
•“Electrical hazards” on page 11
•“Chemicals” on page 12
•“Ergonomics” on page 13
•“Laser hazard” on page 14

1 Safety guidelines
General safety information
2Bravo Automated Liquid-Handling Platform Safety and Installation Guide
General safety information
Before installing and using the Bravo Platform
Before installing and using the Bravo Platform, make sure you are aware of the
potential hazards and understand how to avoid being exposed to them. You
must be properly trained in the correct and safe installation and operation of
the device.
Intended product use
Agilent Technologies products must only be used in the manner described in
the Agilent Technologies product user guides. Any other use may result in
damage to the product or personal injury. Agilent Technologies is not
responsible for any damages caused, in whole or in part, by improper use of
the products, unauthorized alterations, adjustments or modifications to the
products, failure to comply with procedures in Agilent Technologies product
user guides, or use of the products in violation of applicable laws, rules or
regulations. Except as otherwise expressly provided in Agilent Technologies
product user guides, any alteration, adjustment or modification to the products
will void the product warranty and may invalidate the safety compliance
certification.
The Bravo Platform is not intended or approved for diagnosis of disease in
humans or animals. You assume full responsibility for obtaining any regulatory
approvals required for such use and assume all liability in connection
therewith.
Safety labels
Pay attention to any safety labels affixed to your device. A safety label consists
of a warning symbol that indicates a risk of danger. A description of the
warning and information that will help you to avoid the safety hazard are
provided in this user guide.
The following figure shows the location of the warning label on the Bravo
Platform.
WARNING Do not remove the Bravo Platform exterior covers or otherwise
disassemble the system or device. Doing so can expose you to hazards that could cause
serious injury and damage the Bravo Platform.
WARNING Using controls, making adjustments, or performing procedures other than
those specified in the user guide can expose you to moving parts, hazardous voltage, and
laser radiation.

3
1 Safety guidelines
General safety information
Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Figure Bravo Platform warning label location
Warnings in the user documentation or on the device must be observed during
all phases of operation, service, and repair of this device. Failure to comply
with these precautions violates safety standards of design and the intended use
of the product. Agilent Technologies assumes no liability for the customer’s
failure to comply with these requirements.
The following table lists the common symbols you might find on the device.
The symbol on the label indicates the risk of danger. A description of the
warning and information that will help you avoid the safety hazard are
provided in this guide.
Symbol Description
Indicates that you must read the accompanying
instructions (for example, the safety guide) for more
information before proceeding.
Indicates hazardous voltages.
Indicates pinch, crush, and cut hazard.
Indicates laser hazard.

1 Safety guidelines
General safety information
4Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Related information
Indicates hot surface hazard.
Indicates protective conductor terminal, which is bonded
to conductive parts of an equipment for safety purposes.
Indicates that you must not discard this electrical/
electronic product in domestic household waste.
Symbol Description
For information about… See…
Stopping the Bravo Platform in an
emergency
“Emergency stops” on page 7
Moving parts hazards “Mechanical hazards” on page 8
Hazardous voltage “Electrical hazards” on page 11
Chemical hazards “Chemicals” on page 12
Lifting hazard “Ergonomics” on page 13
Laser hazard “Laser hazard” on page 14
Reporting problems with the Bravo
Platform
“Reporting problems” on page vii

5
1 Safety guidelines
Safety and regulatory compliance
Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Safety and regulatory compliance
Compliance standards
The Bravo Platform complies with the applicable EU Directives and bears the
CE mark. See the Declaration of Conformity or Declaration of Incorporation, as
applicable, for details. The Bravo Platform is designed to comply with the
standards listed in the following table.
Electromagnetic compatibility
If the Bravo Platform causes interference with radio or television reception,
which can be determined by turning the device off and on, try one or more of
the following measures:
•Relocate the radio or television antenna.
•Move the device away from the radio or television.
•Plug the device into a different electrical outlet, so that the device and the
radio or television are on separate electrical circuits.
•Make sure that all peripheral devices are also certified.
•Make sure that appropriate cables are used to connect the device to
peripheral equipment.
•Consult your equipment dealer, Agilent Technologies, or an experienced
technician for assistance.
Changes or modifications not expressly approved by Agilent Technologies could
void the user's authority to operate the equipment.
Sound emission declaration
Sound pressure: Lp < 70 dB according to EN 27779:1991.
Schalldruckpegel: LP < 70 dB nach EN 27779:1991.
Regulatory Compliance Standard
EMC
European Union EMC Directive 2004/108/EC
IEC 61326- 1:2005 / EN 61326- 1:2006
Canada ICES/NMB- 001:2004
Australia/New Zealand AS/NZS CISPR 11:2004
Safety
European Union Machinery Directive 2006/42/EC
Low Voltage Directive 2006/95/EC
IEC 61010- 1:2001 / EN61010- 1:2001
Canada CAN/CSA- C22.2 No. 61010- 1- 04
USA ANSI/UL 61010- 1:2004

1 Safety guidelines
Safety and regulatory compliance
6Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Related information
For information about… See…
General safety “General safety information” on page 2
Stopping the Bravo Platform in an
emergency
“Emergency stops” on page 7
Moving parts hazards “Mechanical hazards” on page 8
Hazardous voltage “Electrical hazards” on page 11
Chemical hazards “Chemicals” on page 12
Lifting hazard “Ergonomics” on page 13
Laser hazard “Laser hazard” on page 14
Reporting problems with the Bravo
Platform
“Reporting problems” on page vii

7
1 Safety guidelines
Emergency stops
Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Emergency stops
Procedure
To stop in an emergency:
Press the red button on the robot- disable pendant. The power is cut from the
motors, causing all motion to stop.
Figure Robot-disable pendant
Related information
WARNING If the Bravo Platform is integrated with other devices in a system, Agilent
Technologies recommends that you install a main emergency stop button to cut power to
all devices simultaneously. In addition, all operators must be instructed in the emergency
stop procedure.
CAUTION If the red button on the pendant is pressed while the pipette head is aspirating
or dispensing, the pipetting accuracy might be impaired. Do not use the pendant to pause and
continue a run.
For information about… See…
General safety “General safety information” on page 2
Regulatory compliance “Safety and regulatory compliance” on
page 5
Moving parts hazards “Mechanical hazards” on page 8
Hazardous voltage “Electrical hazards” on page 11
Chemical hazards “Chemicals” on page 12
Lifting hazard “Ergonomics” on page 13
Laser hazard “Laser hazard” on page 14
Reporting problems with the Bravo
Platform
“Reporting problems” on page vii

1 Safety guidelines
Mechanical hazards
8Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Mechanical hazards
Moving parts hazard
The Bravo Platform has moving parts that are accessible at the front, sides,
and rear of the device.
The following figures and tables show the potential moving- parts hazards.
Figure Bravo Platform (top front view) showing moving-parts hazards
00232
Bravo
Pinch Hazard
00232
X
Y
2
1
3
X
Item Part Axis of
motion
Description
1Tie bar x- axis The tie bar is attached to the arm and moves
side to side across the front of the deck.
2Arm x- axis The arm carries the head mount side to side
across the deck.
3Head mount y- axis The pipette head attaches to the head mount,
which moves back and forth on the arm
between the back and front of the deck.
z- axis The head mount raises and lowers the pipette
head.

9
1 Safety guidelines
Mechanical hazards
Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Figure Moving parts on Bravo gripper assembly
Mitigating the risk to users
The Bravo Platform has a safety interlock circuit that must be closed for the
device to operate. Interrupting the safety interlock circuit will stop the motion
of the robot head instantly. Agilent Technologies highly recommends that you
connect the Bravo safety interlock circuit to a light curtain or an enclosure
with an interlock switch to mitigate the risk from moving parts.
If the Bravo Platform is operated outside an enclosure, you should install
safety shields, as appropriate, in addition to a light curtain at the front of the
device. See “Installing the Light Curtain and front and side shields” on page 32
and “Installing the rear shield” on page 44.
It is the responsibility of every operator to follow the warnings and safety
labels and to keep away from the Bravo Platform whenever the device is likely
to move.
Zg
G
4
Item Part Axis of
motion
Description
4Gripper
assembly
G- axis The grippers close and open to grip and
release labware.
Zg- axis The grippers moves up to provide clearance
when the head is moving across the deck,
and down to extend beyond the pipette head
to pick and place labware.
WARNING To reduce the risk of injury from moving parts, ensure that the Bravo safety
interlock circuit is connected to a light curtain or an enclosure with an interlock switch.
WARNING If you touch any of the moving parts or attempt to move labware while the
Bravo Platform is in operation, the device could pinch, pierce, or bruise you. Keep your
fingers, hair, clothing, and jewelry away from the device while it is in motion.

1 Safety guidelines
Mechanical hazards
10 Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Related information
WARNING The pipette head’s z-axis motor is particularly powerful. It might not stop
immediately in a collision and a pipette tip could pierce your hand. Keep away from the
Bravo Platform when the pipette head is moving or about to move, especially in the z-axis
direction.
WARNING When you initialize the Bravo Platform, the head and tie bar can move. To
prevent injury, keep clear of the device while it is in motion.
WARNING Connecting the Bravo Platform to a company or general network can
potentially cause injury. Remote computer operators might accidently initiate an operation
that causes the robot to move unexpectedly, possibly injuring nearby lab personnel. Avoid
connecting the Bravo Platform to a company or general network. Ensure that anyone with
access to the Bravo Platform is trained in the potential hazards and how to avoid them.
For information about… See…
General safety “General safety information” on page 2
Regulatory compliance “Safety and regulatory compliance” on
page 5
Stopping the Bravo Platform in an
emergency
“Emergency stops” on page 7
Hazardous voltage “Electrical hazards” on page 11
Chemical hazards “Chemicals” on page 12
Lifting hazard “Ergonomics” on page 13
Laser hazard “Laser hazard” on page 14
Reporting problems with the Bravo
Platform
“Reporting problems” on page vii

11
1 Safety guidelines
Electrical hazards
Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Electrical hazards
Hazardous-voltage electronics
Hazardous- voltage electronics can be found within the Bravo Platform. Under
normal operating conditions, you are protected from exposure to the hazardous
voltage.
Hazardous- voltage electronics can also be found in the computer. See the
computer manufacturer documentation for the hazard warnings. Make sure you
follow the instructions on the safe operation of the computer.
Related information
WARNING Do not try to gain access to the interior of the Bravo Platform. Do not
remove panels for any reason. Exposure to the interior electronics of a device can cause
severe injury.
WARNING Ensure that the power cords are in good condition and are not frayed. Use of
frayed or damaged power cords can cause injury. Use of incorrect power cords can cause
damage to the device.
WARNING Shut down the Bravo Platform and unplug the power cord before cleaning,
performing maintenance, or installing accessories on the device.
For information about… See…
General safety “General safety information” on page 2
Regulatory compliance “Safety and regulatory compliance” on
page 5
Stopping the Bravo Platform in an
emergency
“Emergency stops” on page 7
Moving parts hazards “Mechanical hazards” on page 8
Chemical hazards “Chemicals” on page 12
Lifting hazard “Ergonomics” on page 13
Laser hazard “Laser hazard” on page 14
Reporting problems with the Bravo
Platform
“Reporting problems” on page vii

1 Safety guidelines
Chemicals
12 Bravo Automated Liquid-Handling Platform Safety and Installation Guide
Chemicals
Chemical hazards
Some chemicals used when working with the Bravo Platform can be hazardous.
Make sure you:
•Follow standard laboratory procedures and cautions when working with
chemicals.
•Follow your local, state, and federal safety regulations when using and
disposing of the chemicals.
Related information
WARNING Some chemicals used when working with the Bravo Platform can be
hazardous. Wear gloves when cleaning unknown substances from the Bravo deck.
For information about… See…
General safety “General safety information” on page 2
Regulatory compliance “Safety and regulatory compliance” on
page 5
Stopping the Bravo Platform in an
emergency
“Emergency stops” on page 7
Moving parts hazard “Mechanical hazards” on page 8
Hazardous voltage “Electrical hazards” on page 11
Lifting hazard “Ergonomics” on page 13
Laser hazard “Laser hazard” on page 14
Reporting problems with the Bravo
Platform
“Reporting problems” on page vii