Agilent Technologies 1260 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II
Preparative Autosampler
User Manual

Agilent 1260 Infinity II Preparative Autosampler User Manual
Notices
© Agilent Technologies, Inc. 2017
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or transla-
tion into a foreign language) without prior
agreement and written consent from Agi-
lent Technologies, Inc. as governed by
United States and international copyright
laws.
Manual Part Number
G7157-90000
Edition
09/2017
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a
license and may be used or copied only in
accordance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of
a U.S. Government prime contract or sub-
contract, Software is delivered and licensed
as “Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in
FAR 2.101(a) or as “Restricted computer
software” as defined in FAR 52.227-19
(June 1987) or any equivalent agency regu-
lation or contract clause. Use, duplication
or disclosure of Software is subject to Agi-
lent Technologies’ standard commercial
license terms, and non-DOD Departments
and Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully
understood and met.

Agilent 1260 Infinity II Preparative Autosampler User Manual 3
In This Book:
In This Book:
This manual covers the following module:
•Agilent 1260 Infinity II Preparative Autosampler
1Introduction
This chapter gives an introduction to the module and instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Using the Module
This chapter provides information on how to use the module.
4 Installation of the Optional Sample Cooler
This chapter describes the installation of the optional sample cooler.
5 Preparing the Module
This chapter explains the operational parameters of the module.
6 Optimizing Performance
This chapter provides information on how to optimize the module.
7 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1260 Infinity II Preparative Autosampler User Manual
In This Book:
9 Maintenance
This chapter describes the maintenance of the module.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent InfinityLab
LC Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity II Preparative Autosampler User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Features 11
Overview of the Module 12
Operating Principle 13
Leak and Waste Handling 22
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
Specifications of the Sample Cooler 32
3 Using the Module 35
Magnets 36
Turn on/off 37
Status Indicators 39
Exchange Drawers 40
Install the External Tray 42
Sample Trays 44
Choice of Vials and Caps 48
Transporting the Sampler with a Sample Cooler Installed 52
Agilent Local Control Modules 54
4 Installation of the Optional Sample Cooler 57
Injector Step Commands 58
Damaged Packaging 59
Install the Sample Cooler 60
Using the Sample Cooler in an Infinity II Sampler 65

6 Agilent 1260 Infinity II Preparative Autosampler User Manual
Contents
5 Preparing the Module 73
Leak and Waste Handling 74
Preparing the Module 75
Solvent Information 76
Capillary Color Coding Guide 82
Swage Fittings 83
Flow Connections to the sampler 85
Setting up the Sampler 87
6 Optimizing Performance 97
Optimization for Lowest Carry-over 98
Fast Injection Cycle and Low Delay Volume 102
Precise Injection Volume 104
Choice of Rotor Seal 106
7 Troubleshooting and Diagnostics 107
Overview of the Module’s Indicators and Test Functions 108
Maintenance Functions 110
Step Commands 119
Test s 120
Troubleshooting 121
Troubleshooting Guide for the Sample Transport Assembly 122
Agilent Lab Advisor Software 125
8 Error Information 127
What are Error Messages 129
General Error Messages 130
Sampler Error Messages 136
Sample Cooler Error Messages 144

Agilent 1260 Infinity II Preparative Autosampler User Manual 7
Contents
9 Maintenance 147
Introduction to Maintenance 148
Warnings and Cautions 149
Overview of Maintenance 151
Cleaning the Module 152
Remove and Install Doors 153
Exchange the Needle Assembly 154
Exchange the Needle Seat Assembly 159
Exchange the Sample Loop Assembly 164
Exchange the Rotor Seal 167
Exchange the Metering Seal and Piston 173
Exchange the Gripper Arm 177
Replace the Peristaltic Pump Cartridge 179
Exchange the Wash Port Assembly 182
Replace the Module Firmware 187
10 Parts and Materials for Maintenance 189
Main Assemblies 190
Standard Parts 191
Drawer Assembly 192
External Tray 194
Analytical-Head Assembly (900 µL) 195
Standard Sampler Accessory Kit 196
Multi-Draw Kit 197
Additional Part List 198
11 Identifying Cables 199
Cable Overview 200
Analog Cables 202
Remote Cables 204
CAN/LAN Cables 208
RS-232 Cables 209
USB 210

8 Agilent 1260 Infinity II Preparative Autosampler User Manual
Contents
12 Hardware Information 211
Firmware Description 212
Electrical Connections 215
Interfaces 217
Setting the 6-bit Configuration Switch 225
Instrument Layout 229
Early Maintenance Feedback (EMF)230
13 LAN Configuration 231
What You Have to Do First 232
TCP/IP parameter configuration 233
Configuration Switches 234
Initialization Mode Selection 235
Dynamic Host Configuration Protocol (DHCP) 237
Manual Configuration 240
PC and Agilent ChemStation Setup 244
14 Appendix 253
General Safety Information 254
Waste Electrical and Electronic Equipment Directive 261
Refrigerant 262
Radio Interference 264
Sound Emission 265
Solvent Information 266
Agilent Technologies on Internet 267

9
Agilent 1260 Infinity II Preparative Autosampler User Manual
Agilent Technologies
1
Introduction
Product Description 10
Features 11
Overview of the Module 12
Operating Principle 13
Sampling Sequence 13
Needle Parkstation 18
Hydraulic Box 19
Transport Assembly 21
Leak and Waste Handling 22
Leak Sensor 22
Waste Concept 23
This chapter gives an introduction to the module and instrument overview.

10 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Product Description
Product Description
The 1260 Infinity II Preparative Autosampler is designed for laboratories that
need automated sample injection to increase daily throughput of purified
sample. This autosampler is easy to use, reliable, and capable of injecting
sample volumes from vials in the microliter to milliliter range.
Figure 1 Overview of the sampler
Status indicator
Needle assembly
Drawer
Column shelf
Leak drain
Power switch