Agilent Technologies 1260 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II
Preparative Autosampler
User Manual

Agilent 1260 Infinity II Preparative Autosampler User Manual
Notices
© Agilent Technologies, Inc. 2017
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Manual Part Number
G7157-90000
Edition
09/2017
Printed in Germany
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Agilent 1260 Infinity II Preparative Autosampler User Manual 3
In This Book:
In This Book:
This manual covers the following module:
•Agilent 1260 Infinity II Preparative Autosampler
1Introduction
This chapter gives an introduction to the module and instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Using the Module
This chapter provides information on how to use the module.
4 Installation of the Optional Sample Cooler
This chapter describes the installation of the optional sample cooler.
5 Preparing the Module
This chapter explains the operational parameters of the module.
6 Optimizing Performance
This chapter provides information on how to optimize the module.
7 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1260 Infinity II Preparative Autosampler User Manual
In This Book:
9 Maintenance
This chapter describes the maintenance of the module.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent InfinityLab
LC Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity II Preparative Autosampler User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Features 11
Overview of the Module 12
Operating Principle 13
Leak and Waste Handling 22
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
Specifications of the Sample Cooler 32
3 Using the Module 35
Magnets 36
Turn on/off 37
Status Indicators 39
Exchange Drawers 40
Install the External Tray 42
Sample Trays 44
Choice of Vials and Caps 48
Transporting the Sampler with a Sample Cooler Installed 52
Agilent Local Control Modules 54
4 Installation of the Optional Sample Cooler 57
Injector Step Commands 58
Damaged Packaging 59
Install the Sample Cooler 60
Using the Sample Cooler in an Infinity II Sampler 65

6 Agilent 1260 Infinity II Preparative Autosampler User Manual
Contents
5 Preparing the Module 73
Leak and Waste Handling 74
Preparing the Module 75
Solvent Information 76
Capillary Color Coding Guide 82
Swage Fittings 83
Flow Connections to the sampler 85
Setting up the Sampler 87
6 Optimizing Performance 97
Optimization for Lowest Carry-over 98
Fast Injection Cycle and Low Delay Volume 102
Precise Injection Volume 104
Choice of Rotor Seal 106
7 Troubleshooting and Diagnostics 107
Overview of the Module’s Indicators and Test Functions 108
Maintenance Functions 110
Step Commands 119
Test s 120
Troubleshooting 121
Troubleshooting Guide for the Sample Transport Assembly 122
Agilent Lab Advisor Software 125
8 Error Information 127
What are Error Messages 129
General Error Messages 130
Sampler Error Messages 136
Sample Cooler Error Messages 144

Agilent 1260 Infinity II Preparative Autosampler User Manual 7
Contents
9 Maintenance 147
Introduction to Maintenance 148
Warnings and Cautions 149
Overview of Maintenance 151
Cleaning the Module 152
Remove and Install Doors 153
Exchange the Needle Assembly 154
Exchange the Needle Seat Assembly 159
Exchange the Sample Loop Assembly 164
Exchange the Rotor Seal 167
Exchange the Metering Seal and Piston 173
Exchange the Gripper Arm 177
Replace the Peristaltic Pump Cartridge 179
Exchange the Wash Port Assembly 182
Replace the Module Firmware 187
10 Parts and Materials for Maintenance 189
Main Assemblies 190
Standard Parts 191
Drawer Assembly 192
External Tray 194
Analytical-Head Assembly (900 µL) 195
Standard Sampler Accessory Kit 196
Multi-Draw Kit 197
Additional Part List 198
11 Identifying Cables 199
Cable Overview 200
Analog Cables 202
Remote Cables 204
CAN/LAN Cables 208
RS-232 Cables 209
USB 210

8 Agilent 1260 Infinity II Preparative Autosampler User Manual
Contents
12 Hardware Information 211
Firmware Description 212
Electrical Connections 215
Interfaces 217
Setting the 6-bit Configuration Switch 225
Instrument Layout 229
Early Maintenance Feedback (EMF)230
13 LAN Configuration 231
What You Have to Do First 232
TCP/IP parameter configuration 233
Configuration Switches 234
Initialization Mode Selection 235
Dynamic Host Configuration Protocol (DHCP) 237
Manual Configuration 240
PC and Agilent ChemStation Setup 244
14 Appendix 253
General Safety Information 254
Waste Electrical and Electronic Equipment Directive 261
Refrigerant 262
Radio Interference 264
Sound Emission 265
Solvent Information 266
Agilent Technologies on Internet 267

9
Agilent 1260 Infinity II Preparative Autosampler User Manual
Agilent Technologies
1
Introduction
Product Description 10
Features 11
Overview of the Module 12
Operating Principle 13
Sampling Sequence 13
Needle Parkstation 18
Hydraulic Box 19
Transport Assembly 21
Leak and Waste Handling 22
Leak Sensor 22
Waste Concept 23
This chapter gives an introduction to the module and instrument overview.

10 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Product Description
Product Description
The 1260 Infinity II Preparative Autosampler is designed for laboratories that
need automated sample injection to increase daily throughput of purified
sample. This autosampler is easy to use, reliable, and capable of injecting
sample volumes from vials in the microliter to milliliter range.
Figure 1 Overview of the sampler
Status indicator
Needle assembly
Drawer
Column shelf
Leak drain
Power switch

Agilent 1260 Infinity II Preparative Autosampler User Manual 11
Introduction
1
Features
Features
Purification efficiency
•Needle flush port enables rinsing the outside of the needle for lowest
carryover
•Low internal volume means minimum contribution to total internal volume
of the system
Instrument efficiency
•Fully automated vial sampler for reliable injection of sample volumes from
0.1 L to 3.6 mL
•Support of flow rates up to 100 mL/min at 400 bar
Laboratory efficiency
•Maximum capacity of 132 sample vials within the same foot print
•Flexible and convenient sample handling with different types of sample
containers

12 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Overview of the Module
Overview of the Module
The Agilent 1260 Infinity II Preparative Autosampler is designed for use with
other modules of the Agilent 1200 Infinity Series, 1200 Series, and 1100 Series
LC, or with other LC systems if adequate remote control inputs and outputs
are available. The Preparative Autosampler is controlled by Agilent control
software (OpenLAB CDS).
Three sample-rack sizes are available for the Preparative Autosampler. The
standard half-size rack holds 2× 66 vials (2.0 mL) while the other half-size rack
options provide space for 2× 18 vials (6.0 mL) respectively. Any two half-size
rack drawers can be installed in the autosampler simultaneously. A specially
designed two half-size sample-rack holding 2× 50 vials (2.0 mL) vials is
available for method compatibility. These special 2× 50 vials (2.0 mL) half-size
racks are not designed for combinations with the other rack portfolio.
The preparative autosampler's transport mechanism uses an X-Z-Theta
movement to optimize vial pick-up and return. Vials are picked up by the
gripper arm, and positioned below the needle station. The gripper transport
mechanism, the needle station, and the hydraulic unit are driven by motors.
Movement is monitored by optical sensors and optical encoders to ensure
correct operation. The metering device is always flushed after injection to
ensure minimum carry-over.
The 1260 Infinity II Preparative Autosampler injection head provides volumes
from 0.1 – 900 L and can be operated up to 400 bar.
The six- port injection valve unit (only 5 ports are used) is driven by a
high- speed hybrid stepper motor. During the sampling sequence, the valve
unit bypasses the autosampler, and directly connects the flow from the pump
to the column. During injection and analysis, the valve unit directs the flow
through the autosampler. This ensures that all the sample is completely
injected into the column. Any sample residue is removed from the metering
device and from the needle before the next sampling sequence begins.
For applications that require control of the vial temperature, the module can
be combined with the Agilent 1290 Infinity Sample Cooler (G7167-60005). The
combination of the Preparative Autosampler with the sample cooler is called a
"cooled Preparative Autosampler". For more details, refer to the 1290 Infinity
Sample Cooler documentation.

Agilent 1260 Infinity II Preparative Autosampler User Manual 13
Introduction
1
Operating Principle
Operating Principle
Sampling Sequence
The preparative autosampler processor continuously monitors the movements
of the preparative autosampler components during the sampling sequence.
The processor defines specific time windows and mechanical ranges for each
movement. If a specific step of the sampling sequence can’t be completed
successfully, an error message is generated.
During the sampling sequence, the solvent bypasses the preparative sampler
via the injection valve. The gripper arm selects the sample vial, either from a
static sample rack, or from external vial positions. The gripper arm places the
sample vial below the injection needle. The required volume of sample is
drawn into the sample loop by the metering device. Sample is applied to the
column when the injection valve returns to the mainpass position at the end of
the sampling sequence.
The sampling sequence occurs in the following order:
1The injection valve switches to the bypass position.
2The piston of the metering device moves to the initialization position.
3The gripper arm moves from the home position, and selects the vial. At the
same time, the needle lifts out of the seat.
4The gripper moves into the needle station and stops in the draw position.
1
5The needle lowers into the vial.
6The metering device draws the defined sample volume.
7The needle lifts out of the vial.
8The gripper arm moves out slightly and stops in the wash position
1
.
9The needle moves downwards and dips into the wash well of the wash port.
Simultaneously the peristaltic pump delivers the flush solvent.
1
10 The needle moves back.
1
1
only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the
sample vial directly below the needle (Step 4) and lowers the needle into the vial.

14 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Operating Principle
11 The gripper arm moves out of the needle station and the wash port snaps
back in position.
12 The gripper arm replaces the vial, and returns to the home position.
Simultaneously, the needle lowers into the seat.
13 The injection valve switches to the mainpass position.
Injection Sequence
Before the start of the injection sequence, and during a preparative run, the
injection valve is in the mainpass position (see Figure 2 on page 14). In this
position, the mobile phase flows through the autosamplers metering device,
sample loop, and needle. This ensures that all parts in contact with sample are
flushed during the run, thus minimizing carry-over.
Figure 2 Mainpass Position - standard position during runs and when the sampler
is idle
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Agilent 1260 Infinity II Preparative Autosampler User Manual 15
Introduction
1
Operating Principle
When the sample sequence begins, the valve unit switches to the bypass
position (see Figure 3 on page 15). Solvent from the pump enters the valve
unit at port 1, and flows directly to the column through port 6.
Figure 3 Valve in bypass - needle in vial, metering device aspirates sample volume
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16 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Operating Principle
Then the vial is positioned below the needle. The needle moves down into the
vial, the metering unit draws the required sample volume into the loop, and
the needle is raised. In the next step, the needle is washed (see Figure 4 on
page 16).
Figure 4 Outer face of needle getting washed in wash port
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Agilent 1260 Infinity II Preparative Autosampler User Manual 17
Introduction
1
Operating Principle
When the metering unit has drawn the required volume of sample into the
sample loop, the vial is replaced in the sample tray. The wash port flips into
the origin position, the needle is lowered into the needle seat, and the
injection valve switches back to mainpass position, flushing the sample onto
the column (see Figure 5 on page 17).
Figure 5 Valve switches to main pass - sample is transferred towards the LC column
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18 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Operating Principle
Needle Parkstation
The needle parkstation comprises two main assemblies: needle drive and wash
port.
Figure 6 Needle Station
Needle-Drive
The needle movement is driven by a stepper motor connected to the spindle
assembly by a toothed belt. The circular motion of the motor is converted to
linear motion by the drive nut on the spindle assembly. The upper and lower
needle positions are detected by reflection sensors on the needle station
board, while the needle-in-vial position is determined by counting the motor
steps from the upper needle-sensor position.

Agilent 1260 Infinity II Preparative Autosampler User Manual 19
Introduction
1
Operating Principle
Hydraulic Box
The hydraulic box comprises two main assemblies: metering device, and
injection valve.
Figure 7 Hydraulic Unit
Analytical Head
The analytical head is driven by the stepper motor that is connected to the
drive shaft by a toothed belt. The drive nut on the spindle converts the circular
movement of the spindle to linear motion. The drive nut pushes the sapphire
piston against the tension of the spring into the analytical head. The base of
the piston sits on the large bearing of the drive nut, which ensures the piston
is always centered. A ceramic ring guides the movement of the piston in the
analytical head. The home position of the piston is sensed by an optical sensor
on the hydraulic unit board while the sample volume is determined by
counting the number of steps from the home position. The backward
movement of the piston (driven by the spring) draws sample from the vial.
NOTE
The replacement hydraulic box excludes the injection valve and metering head assemblies.

20 Agilent 1260 Infinity II Preparative Autosampler User Manual
1 Introduction
Operating Principle
Injection Valve
The two-position 6-port injection valve is driven by a stepper motor. Only five
of the six ports are used (port 3 is not used). A lever/slider mechanism
transfers the movement of the stepper motor to the injection valve. Two
microswitches monitor switching of the valve (bypass and mainpass end
positions). No valve adjustments are required after replacing internal
components.