Agilent Technologies PL-GPC 220 User manual

Agilent Technologies
Agilent PL-GPC 220
Integrated HT-GPC/SEC
System
User Manual
PL-GPC 220 User Manual

PL-GPC 220 User Manual
Notices
© Agilent Technologies, Inc. 2012
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G7820-90220
Edition
09/2012
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

PL-GPC 220 User Manual 3
In This Guide...
In This Guide...
This manual contains information on the Agilent PL-GPC 220 Integrated
HT-GPC/SEC System (G7820A)
1 Introduction to PL-GPC 220
This chapter gives an instrument overview and an introduction to the function
of the PL-GPC 220.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Unpacking the Instrument
This chapter gives information on how to unpack your PL-GPC 220.
4 Using the Module
This chapter provides information on how to set up the instrument for an
analysis and explains the basic settings.
5 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Maintenance
This chapter provides general information on maintenance of the PL-GPC 220.

4PL-GPC 220 User Manual
In This Guide...
8 Parts for Maintenance
This chapter provides information on parts for maintenance.
9 Appendix
This chapter provides addition information on safety, legal and web.

PL-GPC 220 User Manual 5
Contents
Contents
1 Introduction to PL-GPC 220 7
Introduction 8
System Description and General Operation 9
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3 Unpacking the Instrument 25
Unpacking the Instrument 26
4 Using the Module 29
The Graphical User Interface 30
Setting Up the PL-GPC 220 45
Running Samples on the PL-GPC 220 53
5 Troubleshooting and Diagnostics 55
Diagnostics 56
General Troubleshooting Guide 58
6 Error Information 63
Errors 64
7 Maintenance 69
Introduction 70
Warnings and Cautions 71
Cleaning 73
Decontamination 74
Testing Overtemperature Protection Systems 75
Testing Vapor Sensors 81

6PL-GPC 220 User Manual
Contents
Testing Solvent Level Sensors 86
Testing Oven Door Interlock 88
Laser Interlock Switch 89
Degasser Test 90
Back Pressure Regulator (BPR) 91
Differential Refractive Index Detector 93
Autosampler 97
8 Parts for Maintenance 103
Parts List 104
Pump Spares 105
9 Appendix 107
General Safety Information 108
The Waste Electrical and Electronic Equipment Directive 113
Radio Interference 114
Sound Emission 115
Agilent Technologies on Internet 116

7
PL-GPC 220 User Manual
Agilent Technologies
1
Introduction to PL-GPC 220
Introduction 8
System Description and General Operation 9
Instrument Overview 9
Solvent Module 11
Column Oven 13
PL-GPC 220 Autosampler 15
Injection Sequence 17
This chapter gives an instrument overview and an introduction to the function
of the PL-GPC 220.

8PL-GPC 220 User Manual
1Introduction to PL-GPC 220
Introduction
Introduction
The Agilent PL-GPC 220 Integrated HT GPC/SEC System is a fully automated
chromatograph designed specifically for Gel Permeation Chromatography
(GPC) / Size Exclusion Chromatography (SEC). The instrument features a
40-position carousel, high sensitivity refractive index detector and a reliable
isocratic solvent delivery system housed uniquely in a temperature controlled
enclosure. The open architecture of the column oven enables multi-detection
options both inside and outside of the oven. Although the system was designed
for prolonged high temperature operation, the system delivers an equal level
of performance across the whole of its temperature operating range (30 –
220 °C).
Figure 1 Front View of the Agilent PL-GPC 220 System

PL-GPC 220 User Manual 9
Introduction to PL-GPC 220 1
System Description and General Operation
System Description and General Operation
Instrument Overview
Figure 2 Overview (modules)
&
'
(
)
*
+
1 Control drawer
2Autosampler
3 Main oven module
4 Solvent delivery module
5Pump
6 Solvent degasser

10 PL-GPC 220 User Manual
1Introduction to PL-GPC 220
System Description and General Operation
Figure 3 Overview (display and control elements)
&
'
(
)
*
+
,
1 Power switches
2 Oven over-temperature display and control
3 Autosampler over-temperature display and control
4 Temperature and RI display
5 Oven and laser safety interlock switches
6 Status indicator
7Powerindicators