Agilent Technologies 3100 OFFGEL Fractionator User manual

Agilent Technologies
Agilent 3100
OFFGEL Fractionator
User Manual

OFFGEL User Manual
Notices
© Agilent Technologies, Inc. 2010
No part of this manual may be reproduced in
any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
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Manual Part Number
G3100-90001
Edition
04/2010
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
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Safety Notices
CAUTION
A CAUTION notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated condi-
tions are fully understood and
met.
Microsoft ®is a U.S. registered trademark
of Microsoft Corporation.
Software Revision
This guide is valid for B.06.30 or higher revi-
sions of the Agilent 3100 OFFGEL Fraction-
ator Firmware, where the last two digits of
the Firmware revision refer to minor revi-
sions of the Firmware that do not affect the
technical accuracy of this guide.

OFFGEL User Manual 3
In This Guide…
This book describes how to operate the Agilent 3100 OFFGEL
Fractionator including the browser based user interface.
Chapter Overview
1 Product overview
This chapter gives an overview of the Agilent 3100 OFFGEL
Fractionator. It describes the intended use and the
fractionation principles of the instrument, highlights its
features and outlines the different instrument components.
2 Site requirements and specifications
This chapter describes information about site requirements that
have to be met so that the Agilent 3100 OFFGEL Fractionator
performs within specifications.
Control PC
3100 OFFGEL Fractionator

4OFFGEL User Manual
3 PC, Browser and LAN Requirements
This chapter describes information about requirements for your
PC, browser and LAN that have to be met for the control of the
Agilent 3100 OFFGEL Fractionator.
4 Installation
This chapter describes how to install the Agilent 3100 OFFGEL
Fractionator.
5Operation
This chapter details how to operate the Agilent 3100 OFFGEL
Fractionator. It familiarizes the user with all control functions
from the internet browser interface. It describes the necessary
steps to perform a fractionation and set up a LAN connection to
a PC.
6 Maintenance
This chapter describes cleaning procedures that should be used
to do maintenance on your instrument at regular intervals or
before every use. In addition, it provides instructions on how
update the firmware.
7Troubleshooting
This chapter provides support for troubleshooting the
instrument and the application.
8Safety
This chapter contains safety information relevant to the Agilent
3100 OFFGEL Fractionator.
9 Appendix
This chapter contains details on non-condensing condition as
well as ordering information for consumables and spare parts.

OFFGEL User Manual 5
Related Documents
The Agilent 3100 OFFGEL Fractionator can be used with
different consumable kits which each are supplied with a Kit
Manual:
•5969-1582 Kit Manual

6OFFGEL User Manual

Contents
OFFGEL User Manual 7
Contents
1 Product overview 13
Intended Use 14
Fractionation Principles of the Agilent 3100 OFFGEL Fractionator 14
Features 16
Instrument and Accessories 17
2 Site requirements and specifications 21
Site Requirements for the Agilent 3100 OFFGEL Fractionator 22
Power Considerations 22
Bench Space 22
Environment 22
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 23
Do not dispose of in domestic household waste 23
Sound Emission 23
Instruction for Use 24
Physical Specifications of the Agilent 3100 OFFGEL Fractionator 25
3 PC, Browser and LAN Requirements 27
PC, Browser and LAN Requirements 28
Browser Interface 28
LAN 29
Tested Configurations 29
4 Installation 31
Unpacking the Agilent 3100 OFFGEL Fractionator 32
Setting up the Agilent 3100 OFFGEL Fractionator 34
Setting up the Control PC 36

8OFFGEL User Manual
Contents
Assignment of an IP Address to the Control Computer 36
Local Network Connection (optional) 37
5Operation39
The User Interface 40
General Functions 42
Status tab 43
Working with Methods 46
Displaying Data Graphically 49
Zoom in Plots 50
Pan / Drag in Plots 50
Switching the Y-Scale in Plots 51
Printing of Plots 51
Instrument Logbook 52
Exporting Results 54
Reviewing Result Files 57
How to find the exported files on the USB stick 57
How to Display Exported Results 58
Instrument Configuration 62
Additional Information 64
Performing a Fractionation 65
Preparing a Tray for the OFFGEL Mode 66
Preparing a Tray for the Standard IPG IEF Mode 66
Starting a Run 68
6 Maintenance 69
Cleaning the Agilent 3100 OFFGEL Fractionator 70
Cleaning the Electrodes 70

Contents
OFFGEL User Manual 9
Regular Cleaning Procedure 70
Cleaning Procedure for Heavily Contaminated Electrodes 70
Cleaning the Trays 71
Cleaning the Cooling Platform 72
Cleaning the Anode Connector 72
Cleaning the Top Cover and the Housing of the Instrument 75
Firmware Update 76
Installation of the Firmware Update Tool 77
How to un-install the Firmware Update Tool 77
Installation of the Firmware on your PC 77
Connect your PC to the Agilent 3100 OFFGEL Fractionator 78
Setup of the Firmware Update Tool 79
Configure the Firmware Update Tool 80
Doing the Firmware Update 84
The Log-file 87
Firmware Change via USB Stick 88
Steps for Firmware Change via USB 89
7 Troubleshooting 91
Troubleshooting the Instrument 92
Status Indicators 92
Status LED 92
Status Bars 93
Error Messages 93
Hardware Diagnosis 96
Checking the LED and the cover sensor 96
Checking Temperature Control of the Cooling Platform 96
Checking High Voltage, Electrodes and Electrode Connectors 97
Troubleshooting the Electrodes 100

10 OFFGEL User Manual
Contents
Checking Fixed and Movable Electrodes 100
Troubleshooting the LAN Connection 101
Troubleshooting the Application 102
Looking at Run Parameters 105
Example 1. Typical Results of the Protein Test Sample 105
Example 2 . Current Drop During Fractionation 107
Example 3. Reduced Electrical Charge in One Lane 108
8Safety113
Safety Information 114
Power Cords 114
Operation 115
Safety Symbols 116
Chemical and Biological Safety 116
8安全性 119
安全性について 120
電源コード 121
操作 121
安全記号 122
化学および生物的な安全性 122
8安全 125
安全说明 126
电源线 126
操作 127
安全标志 127
化学和生物安全 128
8Sicherheit129
Sicherheitshinweise 130