Beckman Coulter Avanti J-E User manual

SJ-IM-8
Avanti®J-E CentrifugeAvanti®J-E Centrifuge
Instruction Manual

Symbol
Symbol
Symbole
Símbolo
Title / Titel / Titre / Titulo / Titolo /
Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!
/
Simbolo

SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, maintenance,
or repair of this centrifuge. When you see the symbol on other pages, pay special attention to
the safety information presented. Observance of safety precautions will also help to avoid
actions that could damage or adversely affect the performance of the centrifuge.
Safety During Installation and/or Maintenance
This centrifuge is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any
warranty covering the instrument.
Any servicing of this equipment that requires removal of any covers can expose parts which
involve the risk of electric shock or personal injury. Make sure that the power switch is off
and the centrifuge is disconnected from the main power source, and refer such servicing to
qualified personnel.
Use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage that could result from instrument
movement in the event of a major rotor mishap.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the centrifuge to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed
to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
Fuses protect certain electrical circuits within this instrument against overcurrent conditions.
For continued protection against the risk of fire, replace only with the same type and rating
specified.
!

This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the required 30-cm (1-ft) area
surrounding the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the rotors and accessories designed for use in this centrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• Do not lift or move the centrifuge while the rotor is spinning.
• NEVER attempt to slow or stop the rotor by hand.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the centrifuge
for at least 1 hour. Then follow the instructions for sample recovery in Section 4,
TROUBLESHOOTING.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent—
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic
fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the centrifuge and accessories before
requesting service by Beckman Coulter field service.

SJ-IM-8
August 2009
A
vant
i
Avanti
®
®
J
-E Centrifug
e
J-E Centrifuge
Instruction Manual
© 2009 Beckman Coulter, Inc.

Contents
iii
Page
INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Typographic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
SECTION 1: DESCRIPTION
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Housing and Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Vacuum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Temperature Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Control and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Protective Ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Imbalance Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Overspeed and Rotor Identification System . . . . . . . . . . . . . . . . . 1-9
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11
SECTION 2: PREINSTALLATION REQUIREMENTS
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Securing the Centrifuge to the Floor . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Bio-Safety Level 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

iv
Contents
Page
SECTION 3: OPERATION
Summary of Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Programmed Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Manual Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Installing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Manual Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Selecting a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Entering Run Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Entering Run Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Entering Run Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Entering Acceleration and Deceleration Rates . . . . . . . . . . . . . . . 3-8
Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Creating a New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Starting a Programmed Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Recalling and Changing a Program . . . . . . . . . . . . . . . . . . . . . . . 3-11
SECTION 4: TROUBLESHOOTING
User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Retrieving Your Sample in Case of Power Failure . . . . . . . . . . . . . . . 4-4
SECTION 5: MAINTENANCE
Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Sterilization and Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Replacing the Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Circuit Breaker and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Storage and Transportation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Warranty

Illustrations and Tables
v
Page
Figure 1-1. The Avanti®J-E Centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Figure 1-2. Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Figure 2-1. Dimensions of the Avanti®J-E Centrifuge . . . . . . . . . . . . . . . . . . . . . 2-2
Figure 2-2. Single-Phase Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Figure 4-1. Manual Door Release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Figure 5-1. Air Filter Replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Table 4-1. Diagnostic Message Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

vii
CERTIFICATION
To ensure full system quality, Beckman Coulter Avanti®J-E centri-
fuges have been manufactured in a registered ISO 9001 or 13485
facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment
requirements of applicable regulatory agencies. Declarations of
conformity and certificates of compliance are available at
www.beckmancoulter.com.
SCOPE OF MANUAL
This manual is designed to familiarize you with the Avanti®J-E
centrifuge, its functions, specifications, operation, and routine
operator care and maintenance. We recommend that you read this
entire manual, especially the SAFETY NOTICE and all safety-related
information, before operating the centrifuge or performing instrument
maintenance.
• Section 1 contains system specifications and a brief physical and
functional description of the centrifuge, including the operating
controls and indicators, and available rotors.
• Section 2 provides information about space and power require-
ments for installing and connecting the centrifuge.
• Section 3 contains centrifuge operating procedures.
• Section 4 lists possible malfunctions, together with probable
causes and suggested corrective actions.
• Section 5 contains procedures for routine operator care and main-
tenance, as well as a brief list of supplies and replacement parts.
Introduction

viii
Introduction
➠NOTE
If the centrifuge is used in a manner other
than specified in this manual, the safety and
performance of this equipment could be
impaired. Further, the use of any equipment
other than that recommended by Beckman
Coulter has not been evaluated for safety. Use of
any equipment not specifically recommended in
this manual and/or the applicable rotor manual is
the sole responsibility of the user.
CONVENTIONS
Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.
NOTES, CAUTIONS, AND WARNINGS
➠NOTE
Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment.
!CAUTION
Used to indicate a potentially hazardous situa-
tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.

ix
Introduction
!WARNING
Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.
WARNING
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
either symbol to service personnel.
TYPOGRAPHIC CONVENTIONS
Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keys.
•Keynames (for example, [START] or [PROGRAM]) appear in capital
letters within brackets.
CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Avanti J-E centrifuges.
CFC

x
Introduction
RADIO INTERFERENCE
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ-
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica-
tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.
CANADIAN REGULATIONS
This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.
RECYCLING LABEL
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:
1) the device was put on the European market after August 13, 2005
and
2) the device is not to be disposed via the municipal waste collection
system of any member state of the European Union.
It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.
A28219-AA
Note: On the instrument, the
triangle background is yellow
rather than gray.

1
1-1
DESCRIPTION
CENTRIFUGE FUNCTION
The Avanti®J-E high-performance centrifuge (see Figure 1-1) is a
refrigerated centrifuge that generates centrifugal forces required for a
wide variety of applications. Together with the compatible Beckman
Coulter rotors, applications include:
• Routine processing such as sample preparations, pelleting, extrac-
tions, purifications, concentrations, phase separations, and spin
column and spin filter centrifugations.
Figure 1-1. The Avanti®J-E Centrifuge
Description

1-2
Description
• Rapid sedimentation of protein precipitates, large particles, and
cell debris.
• Preparation of subcellular organelles such as mitochondria, nuclei,
chloroplasts, and crude microsomes.
• Separation of blood cells and cellular components.
• Gradient separation, for example, Ficoll-Hypaque1and Percoll.1
The Avanti J-E is microprocessor-controlled, providing interactive
operation. The instrument design features a brushless switched-
reluctance drive motor,2a temperature control system with automatic
temperature compensation unique for each compatible rotor, and
selectable acceleration and deceleration rates.
Manual and programmed operation are available.
• In manual operation, you enter the individual run parameters
before beginning each run.
• In programmed operation, you can duplicate runs quickly and
accurately by selecting one of nine programs previously entered
into the program memory.
User messages and/or audible signals are provided to alert you to
conditions that may need attention.
HOUSING AND DOOR
The instrument housing is made of steel and molded structural foam.
The door and structural-foam cover panels are finished with polyure-
thane enamel.
The steel and structural foam door is hinged at the back, providing
clearance for loading and unloading of the centrifuge. In the event of
a power failure, the door can be manually unlocked for sample
recovery (see Section 4, TROUBLESHOOTING).
1Registered trademarks of Pharmacia AB.
2Manufactured under license from Switched Reluctance Drives Limited, Harrogate, U.K.

1-3
Description
ROTOR CHAMBER
The rotor chamber is made of stainless steel to resist corrosion.
A rubber gasket around the chamber opening ensures sealing.
(Instrument gaskets have not been qualified as bioseals for aerosol
containment.)
VACUUM
The friction reduction system (FRS) uses a mechanical rotary vane
vacuum pump to reduce chamber pressure to approximately one-half
atmosphere. The pump turns on when the run is started, before rotor
friction reaches a high level. Vacuum in the chamber is vented auto-
matically during rotor deceleration.
TEMPERATURE CONTROL
The temperature control system cools by circulation of a non-CFC-
based refrigerant. The system is activated when the centrifuge power
is on and when the door is closed and latched.
A thermistor in the rotor chamber continuously monitors the chamber
temperature. The system calculates the chamber temperature required
to maintain the set rotor temperature, ±2°C. Although the chamber
temperature fluctuates during operation, the rotor’s large mass keeps
the sample temperature substantially constant. At the end of a run,
the system continues controlling the temperature for 30 minutes to
prevent freezing or overheating of the sample.
➠NOTE
The system will always shut down using
maximum brake if the temperature goes
above 50°C.

1-4
Description
DRIVE
The drive shaft is belt driven by a brushless, high-torque, switched-
reluctance motor. The instrument’s resilient suspension minimizes
disturbance of the sample during acceleration and deceleration, and
reduces damage to the drive shaft if an imbalance occurs during
centrifugation.
CONTROLS AND INDICATORS
POWER SWITCH
The power switch, located on the right panel of the centrifuge,
controls electrical power to the centrifuge. It is also a circuit breaker
that will trip to cut off power in the event of a power overload. The
power switch must be turned on before the chamber door can be
opened.
CONTROL PANEL
The control panel (see Figure 1-2) is mounted at an angle on the
centrifuge front for easy visibility and access. It is used to enter run
parameters via function keys and a keypad, and to display run param-
eters, program information, and user messages.
Figure 1-2. Control Panel

1-5
Description
Digital Displays
The displays provide run information, user messages, and
diagnostics.
• During a run, they show the actual (real-time) operating
conditions.
• When the run parameters are being entered, they show the set
values selected. Set values can be recalled during operation by
pressing [SHOW SET]. After 5 seconds, the displays return to actual
conditions.
(SPEED) Indicates rotor speed in revolutions per minute (rpm) or in relative
centrifugal field, a term describing the ratio of the centrifugal acceler-
ation at a specified radius and speed to the standard acceleration of
gravity (RCF ×g).
(TIME) • In a timed run, indicates the remaining run time in hours and
minutes. The time display begins counting down when [START]
is pressed and continues counting down to 0, when deceleration
begins. The run automatically ends when the set time reaches zero
(a tone sounds when the rotor stops spinning). If a malfunction
shuts down the centrifuge during a run, the time counting stops and
the time remaining when the run stopped remains in the display.
By comparing this time with the original set time, you
can determine when the run ended.
• In a hold run, indicates the time elapsed since [START] was pressed.
After 99 hours and 59 minutes the timer resets to 0 and continues
counting elapsed time.
(TEMPERATURE) Indicates rotor temperature in degrees C at thermoequilibrium. The
displayed temperature is within ±2 degrees of the set temperature
(after equilibration).
(ROTOR) Displays the name of the installed rotor.
(ACCEL) When the [ACCEL] key is pressed, an acceleration rate (1 = MAX,
2 = SLOW) can be entered. If no number is entered, the instrument
accelerates at the rate set for the previous run.
(DECEL) When the [DECEL] key is pressed, a number representing the selected
deceleration rate (1 = MAX, 2 = SLOW, 3 = OFF) can be entered. If
no number is entered, the instrument decelerates at the rate set for the
previous run.

1-6
Description
(PROGRAM) • During setup—when the [PROGRAM] key is pressed a program
number (one of nine possible) can be entered.
• During centrifugation—the number of the program being run is
displayed.
Run-Parameter Keys
Run-parameter keys are used to enter specific run information as
follows.
Pressed to display scrolling list of compatible rotors and rotor entry
codes. Can be pressed repeatedly to scroll more quickly through the
rotor list.
If the display shows speed in rpm, the key is pressed once to enter run
speed (using the keypad) in rpm (revolutions per minute); pressed
twice to enter run speed in RCF (relative centrifugal field). If the
display shows speed in RCF, the key is pressed once to enter speed
in RCF or twice to enter speed in rpm.
Pressed to enter run time using the keypad. When [TIME] is pressed
twice, HOLD mode is selected. (Pressing it again reverts to time-
entry mode.)
• Timed run—Run time up to 99 hours and 59 minutes can be set. If
more than 59 minutes are entered in the minutes field, the system
automatically converts the entry to hours and minutes. Decelera-
tion begins when the set time counts down to zero.
• Continuous run (hold)—For runs of unspecified lengths, hold
mode is used. When 99 hours, 59 minutes is reached, the system
resets to 0 and continues counting. A hold run continues until the
[STOP] key is pressed.
Pressed to enter run temperature (using the keypad), from –10 to
+40°C. If a new temperature is not entered, the centrifuge uses the
temperature set for the previous run.