Beckman Coulter Avanti JXN-26 User manual

PN B38322AA
February 2014
User Guide
Avanti JXN Series
High Performance Centrifuges
For In Vitro Diagnostic Use
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Avanti JXN
High Performance Centrifuges
User Guide
PN B38322AA (February 2014)
© 2014 Beckman Coulter, Inc.
All Rights Reserved
Avanti, Beckman Coulter, and the stylized logo are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Printed in USA
Beckman Coulter Ireland, Inc.
Mervue Business Park, Mervue Galway, Ireland 353 91 774068

PN B38322AA iii
Revision History
Initial Issue, 02/2014
Avanti JXN-26
Avantii JXN-30
Initial Release
This document applies to the latest software listed and higher versions. When a subsequent software
version affects the information in this document, a new issue will be released to the Beckman Coulter Web
site. For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual
or system help for your instrument.

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Revision History

PN B38322AA v
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Beckman Coulter, Inc. urges its customers and employees to comply with all national health and
safety standards such as the use of barrier protection. This may include, but is not limited to,
protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or
any other automated laboratory analyzer.
Alerts for Danger, Warning, Caution, Important, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis.
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

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Safety Notice
Alerts for Danger, Warning, Caution, Important, and Note
WARNING
Risk of operator injury if:
• All doors, covers, and panels are not closed and secured in place prior to and
during instrument operation.
• The integrity of safety interlocks and sensors is compromised.
• Instrument alarms and error messages are not acknowledged and acted upon.
• You contact moving parts.
• You mishandle broken parts.
• Doors, covers, and panels are not opened, closed, removed, and/or replaced
with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers, and panels closed and secured in place while the
instrument is in use.
• Take full advantage of the safety features of the instrument. Do not defeat
safety interlocks and sensors.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your Beckman Coulter Representative.
• Open/remove and close/replace doors, covers, and panels with care.
• Use the proper tools when troubleshooting.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the Product Manuals.
• You introduce software that is not authorized by Beckman Coulter into your
computer. Only operate your system’s computer with software authorized by
Beckman Coulter.
• You install software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.
CAUTION
If you purchased this product from anyone other than Beckman Coulter or an
authorized Beckman Coulter distributor, and, it is not presently under a Beckman
Coulter service maintenance agreement, Beckman Coulter cannot guarantee that
the product is fitted with the most current mandatory engineering revisions or
that you will receive the most current information bulletins concerning the

PN B38322AA vii
Safety Notice
Safety Notices
product. If you purchased this product from a third party and would like further
information concerning this topic, contact your Beckman Coulter Representative.
Safety Notices
Before installing, using, or maintaining the instrument, be certain that you know all the following
precautions.
Installation
CAUTION
This instrument is designed to be installed by your Beckman Coulter
Representative. Installation by anyone other than authorized Beckman Coulter
personnel invalidates any warranty covering the instrument. Also, should the
instrument need to be moved, your Beckman Coulter Representative must
reinstall and re-level the instrument in its new location.
WARNING
Do not place the centrifuge near areas containing flammable or combustible
fluids, or any other source of vapors that could enter the centrifuge air system and
be ignited by the motor.
WARNING
Risk of operator injury. High performance centrifuge operations generate high
energy levels that require precautions against sudden movements that could
result from the rare event of a rotor failure. Maintain a 30-cm (1-ft) clearance
envelope around and above the centrifuge. Do not install any equipment or
furniture in this envelope. While the centrifuge is running, keep the envelope clear
of any persons or objects and do not reach into the envelope except when required
to change operating controls.
Replacement Parts
CAUTION
Risk of rotor mishap and/or improper instrument function. Do not replace any
centrifuge components with parts not specified for use on this instrument.

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Safety Notice
Safety Notices
Service
WARNING
Any servicing of this equipment that requires removal of any covers can expose
parts which involve the risk of electric shock or personal injury. Make sure that the
power switch is turned off, and the instrument is disconnected from the main
power source, by removing its power plug from the receptacle. Refer such
servicing to qualified personnel.
NOTE It is your responsibility to decontaminate the instrument and accessories before requesting service by
your Beckman Coulter Representative.
Power Supply
DANGER
To reduce the risk of electrical shock, this instrument uses a three-wire electrical
cord and plug to connect this equipment to earth-ground. Make sure that the
matching wall outlet receptacle is properly wired and earth-grounded.
Fuse Replacement
WARNING
Fuses protect certain electrical circuits within this instrument against overcurrent
conditions. The fuse is not customer replaceable. For continued protections,
please contact your Beckman Coulter Representative.

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Safety Notice
Safety Notices
Mechanical Safety
DANGER
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this
instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not move the centrifuge while the drive motor is spinning.
• NEVER attempt to override the door interlock system.
• Do not lean on the centrifuge or place items on it while it is operating.
• In the event of a power failure, do not attempt to retrieve the sample from the
instrument until the rotor has come to a complete stop. The time required for
a rotor to come to a complete stop can vary from 1 to 9 hours depending upon
the set speed while in operation and the rotor’s mass. Then follow the
instructions for recovery of the sample in the Maintenance and
Troubleshooting chapter.
Chemical and Biological Safety
DANGER
Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Such materials require that you take all
necessary safety precautions.
• Handle body fluids with care because they can transmit disease. No known
test offers complete assurance that they are free of micro-organisms.
• Handle all infectious samples according to good laboratory procedures and
methods to prevent spread of disease.
• Because spills may generate aerosols, observe proper safety precautions for
aerosol containment.
• Some of the most virulent infectious agents—Hepatitis (B and C) and HIV (I-V)
viruses, atypical mycobacteria, and certain systemic fungi—require
additional emphasis on aerosol protection.
• Do not run toxic, pathogenic, or radioactive materials in a rotor without taking
appropriate safety precautions.
• Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) require biosafe containment. Materials of a
higher group require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health
and safety guidelines.

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Safety Notice
Summary of Instrument Labels
Precautions with Liquids
CAUTION
Risk of instrument damage. Do not place containers holding liquid on or near the
chamber door. Liquid, if spilled, may get into the instrument and damage
electrical or mechanical components.
Volatile Liquids
DANGER
This instrument is not designed for use with materials capable of developing
flammable or explosive vapors. Do not centrifuge such materials (for example,
chloroform or ethyl alcohol) in this instrument nor handle or store them near the
centrifuge.
Summary of Instrument Labels
This section provides information for some labels and symbols appearing on the Avanti JXN series
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in Warnings or Cautions within the procedures for that task.
Caution Symbol
This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution. Please consult the User Guide to determine the nature of the potential
hazard and any necessary actions to be taken.
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
!

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Safety Notice
Summary of Instrument Labels
China RoHS Caution Label
This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
China RoHS Environmental Label
This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center “e” indicates the product is environmentally safe and does not have an
Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The
circling arrows indicate the product is recyclable. The date code on the label or product indicates
the date of manufacture.
Universal Serial Bus (USB)
This symbol indicates the location of a universal serial bus (USB) connector.
WEEE Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
• the device was put on the European Market after August 13, 2005 and
A28219-AA

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Safety Notice
Summary of Instrument Labels
• the device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
Multiple Compliance
This symbol indicates compliance with:
• IVD – For in vitro diagnostic use
• N395 – The C-Tick mark is intended for use on products that comply with Australian
Communication Authority (ACA) EMC Requirements.
CAUTION
This equipment has been designed and tested to CISPR 11 Class A. In a domestic
environment it could cause radio interference, in which case, you may need to
take measures to mitigate the interference.
It is advised that prior to operation of the device, the electromagnetic
environment should be evaluated. Do not use this device in close proximity to
sources of strong electromagnetic radiation (for example, unshielded intentional
RF sources), as these could interfere with proper operation.
• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.
NOTE 169502 is applicable to North American models only.
• CE – This label indicates conformance to various Directives set forth under European Union law.
• Recycling – Refer to the Recycling Label section in this document.

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Safety Notice
Summary of Instrument Labels
Biohazard Warning
These caution symbols indicate biohazardous risk from possible patient specimen contamination.
CAUTION Static Sensitive Area
Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To
prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap
while operating this instrument. For details on proper grounding, see IEEE standard P1100.
DANGER High Voltage
Operation, replacement or servicing of any components where contact with bare, live hazardous
parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.
Protective Ground
This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.

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Safety Notice
Summary of Instrument Labels
Rotor Rotation
This indicates the direction of instrument rotor rotation.

xv
Contents
Revision History, iii
Safety Notice, v
Alerts for Danger, Warning, Caution, Important, and Note, v
Safety Notices, vii
Installation, vii
Replacement Parts, vii
Service, viii
Power Supply, viii
Fuse Replacement, viii
Mechanical Safety, ix
Chemical and Biological Safety, ix
Precautions with Liquids, x
Volatile Liquids, x
Summary of Instrument Labels, x
Caution Symbol, x
RoHS Notice, x
China RoHS Caution Label, xi
China RoHS Environmental Label, xi
Universal Serial Bus (USB), xi
WEEE Recycling Label, xi
Multiple Compliance, xii
Biohazard Warning, xiii
CAUTION Static Sensitive Area, xiii
DANGER High Voltage, xiii
Protective Ground, xiii
Rotor Rotation, xiv
Introduction, xxvii
Overview, xxvii
About This Manual, xxvii
Conventions, xxviii
Certification, xxviii
Scope of Manual, xxviii

xvi
Contents
CFC-Free Centrifugation, xxix
Software Copyright, xxix
Symbols, xxx
Graphics, xxxi
CHAPTER 1: Description, 1-1
Overview, 1-1
For In Vitro Diagnostic Use, 1-1
Centrifuge Function, 1-2
Touch Screen, 1-2
Name Rating Plate, 1-2
Housing and Door, 1-3
Rotor Chamber, 1-3
Friction Reduction System (FRS), 1-3
Drive, 1-3
Temperature Sensing and Control, 1-3
Overtemp System, 1-4
Safety Features, 1-4
Specifications, 1-5
Audible Sounds, 1-7
Available Rotors, 1-7
CHAPTER 2: The Touch Screen Interface, 2-1
Overview, 2-1
Areas on the Screen, 2-2
Header Bar, 2-2
Home Page Button, 2-2
Menu Button, 2-3
Status Display, 2-3
Help Button, 2-4
Footer Bar, 2-4
Start Button, 2-4
Stop Button, 2-5
Footer Bar on the Home Page, 2-5
Footer Bar on Other Pages, 2-5
Rotor Display/Button, 2-5
System Name, 2-6
Accel and Decel Display/Button, 2-6
Page Display Area, 2-6
Help Messages, 2-6

xvii
Contents
Item Help, 2-6
Global Help, 2-6
Exit from Help, 2-8
About Your Instrument, 2-8
CHAPTER 3: Operations, 3-1
Overview, 3-1
Manual Operation, 3-1
Step 1: Start on the Home Page, 3-2
Step 2: Set the Speed and Rotor, 3-3
Step 3: Set the Acceleration and Deceleration Profiles, 3-4
Step 4: Set the Time, 3-6
Step 5: Set the Temperature, 3-8
Step 6: Start the Run, 3-8
Zonal and Continuous Flow Operation , 3-9
Preparing for the Run, 3-10
Starting the Run, 3-11
Loading the Sample, 3-11
Running the Sample, 3-12
Unloading the Sample, 3-12
Stopping the Run, 3-12
Finishing the Run, 3-12
CHAPTER 4: Configuration, 4-1
Overview, 4-1
Managing Your Network , 4-1
Setting Up the Network , 4-1
Selecting a Printer, 4-2
Setting Up Email , 4-2
Setting Up VNC, 4-3
Enabling API, 4-4
Managing Users, 4-4
Adding Users, 4-5
Requiring Login, 4-5
PIN Expiration and Logout Timer, 4-6
User Options, 4-7
Managing Rotors, 4-7
Adding Rotors, 4-8
Managing Reports, 4-9
User Access, 4-9
Filtering Data, 4-10
Graphing Data, 4-12
Print Data, 4-13
Export Data, 4-14
Auto Print and Auto Export Run History Data, 4-16

xviii
Contents
Enabling Auto Print , 4-16
Enabling Auto Export, 4-16
Enabling Run Comments, 4-17
E-Signature, 4-18
CHAPTER 5: Programs, 5-1
Overview, 5-1
Creating Programs, 5-2
Running Programs, 5-6
Editing Programs, 5-7
Deleting Programs, 5-8
CHAPTER 6: Functional Pages, 6-1
Overview, 6-1
Home Page, 6-3
Home Page (Run in Progress), 6-5
Set Speed Page, 6-6
Select Rotor Page (Library), 6-7
Set Time Page, 6-8
Delay Start Page, 6-9
Set Speed ω²t Time Page, 6-10
Set Temperature Page, 6-11
Set Acceleration/Deceleration Profiles Page, 6-12
Example Acceleration and Deceleration Settings, 6-13
Login Page, 6-13
Select Program Page, 6-14
New/Edit/View Program Page, 6-15
Select Rotor Page (Catalog), 6-17
New/Edit Step Page, 6-18
Program Log Page, 6-19
Export Page, 6-20
Authorize Users Page, 6-21
Import Page, 6-22
Real-Time Run Data/Historical Run Data Page, 6-23
Run Graph Options Page, 6-24
Run History Page, 6-25

xix
Contents
Run History Filter Page, 6-27
Before Run/After Run Comment Page, 6-28
Menu Page, 6-29
System Options Page, 6-30
The Basic Tab, 6-30
The System Tab, 6-31
The Network Tab, 6-32
The Users Tab, 6-33
The Reports Tab, 6-34
Select Language Page, 6-35
User Options Page, 6-36
Max Temperature Offset Page, 6-37
Reset User PIN Page, 6-38
Select Image Page, 6-39
Set Date and Time Page, 6-40
System Log Page, 6-41
Manage Rotors Page, 6-42
Add to Rotor Library Page, 6-43
Diagnostic History Page, 6-44
Set Sound Page, 6-45
Custom Sounds Page, 6-46
Archive Data Page, 6-47
Setup Network Page, 6-48
Select Printer Page, 6-49
Setup Email Page, 6-50
Setup VNC Page, 6-51
Manage Users Page, 6-52
Add/Edit User Page, 6-53
Authorize Programs Page, 6-54
Rotor Catalog Page, 6-55
About Page, 6-56
Zonal Authorization Page, 6-57
Zonal Operation Page, 6-58
CHAPTER 7: Maintenance and Troubleshooting, 7-1
Overview, 7-1

xx
Contents
Field Service, 7-1
Maintenance, 7-1
Cleaning, 7-2
Tube Breakage, 7-3
Decontamination, 7-3
Sterilization and Disinfection, 7-4
Replacing the Air Filter, 7-4
Checking the Vacuum Pump, 7-5
Diagnostics/User Messages, 7-6
Accessing the Rotor in Case of Power Failure, 7-6
JCF-Z Rotor Identification, 7-11
Storage and Transportation, 7-12
Supply List, 7-12
Replacement Parts and Supplies, 7-12
APPENDIX A: Preinstallation Requirements, A-1
Preinstallation Requirements, A-1
Electrical Requirements, A-1
Single and Three-Phase Power Connections, A-2
Additional Requirements for Three-Phase Power
Connections, A-4
Space and Location Requirements, A-4
Using J2 Series Rotors in the Avanti JXN Series, A-5
Checking for Rotor Drive Pins, A-5
Using the JCF-Z Continuous Flow/Zonal Rotor, A-7
Zonal Bracket Kits, A-7
APPENDIX B: Temperature Calibration Procedure, B-1
Introduction, B-1
APPENDIX C: Diagnostics, C-1
Overview, C-1
Diagnostics/User Messages Chart, C-1
APPENDIX D: Third Party Contributions and Legal Notices, D-1
Contributions and Legal Notices, D-1
APPENDIX E: HEPA Filter Replacement Instructions for the Avanti JXN
Series Centrifuges, E-1
HEPA Filter Replacement, E-1