BestScope BS-2010E User manual

Bestscope International Limited
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Biological Microscope
BS-2010E
User Manual
This manual is for users of biological microscope BS-2010E. To ensure the safety, obtain optimum
performance and to familiarize yourself fully with the use of this microscope, it is recommended
strongly that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the microscope for further reference
BestScope International Limited
Eyepiece
Objective
Monocular Head
Coarse Focusing
Knob
Fine Focusing
Knob
Brightness
Adjustment
Knob
Main
Switch
Stage
Condenser
Collector

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1 Appliance
BS-2010E biological microscope is widely used in medical and hygienic establishments for
conventional microscopic examination, general biologic, pathologic and bacteriological studies,
clinical investigations and classroom demonstrations. Designed in a very modern way what can
take convenient and safe to your operations.
2 Specification
2.1 Mechanical tube length：160mm
2.2 Objective：
Magnification Numerical
Aperture
（NA）
Focus
Distance
(mm)
Working
Distanc
e
(mm)
Remark
4X 0.10 31.05 18
10X 0.25 17.13 7
Eyepiece
Objective
Stage
Condenser
Collector
Sliding
Binocular
Head
Coarse
Focusing
Knob
Fine
Focusing
Knob
Brightness
Adjustment
Knob
Main
Switch

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40X 0.65 4.65 0.53
100X 1.25 2.906 0.13
2.3 Eyepiece
TYPE Magnificatio
n
Focus
Distance(mm) Field of view (mm)
Plan Eyepiece 10X 25 Ø18
2.4Total Magnification
Eyepiece 10X 10X 10X 10X
Objective 4X 10X 40X 100X
Total 40X 100X 400X 1000X
2.5 Conjugate Distance: 195mm
2.6 Stage：
BS-2010 D、E: Double Layers Mechanical Stage 116mmX125mm
BS-2010 A、B: Attachable Mechanical Stage 110mmX120mm
BS-2010 C: Plain Stage with Slide Clips 110mmX120mm
2.7 Coarse Focusing range：10mm
2.8 Condenser：
BS-2010 D、E:Abbe NA=1.2with Iris Diaphragm,Rack and Pinion Adjustment
BS-2010 A : Single Lens NA 0.65 with Disc Diaphragm
BS-2010 B : Single Lens NA 0.65 with Iris Diaphragm
BS-2010 C : Abbe NA=1.2with Iris Diaphragm,SpiralAdjustment
2.9 Diameter of Color Filter：Ø32mm
2.11 Illumination ：
BS-2010 A、B、C、D:Incandescent Lamp Illumination 15W,
Brightness Adjuseable
BS-2010 E: LED Lamp Illumination 1W, Brightness Adjuseable
3 Outfits
Component
Name
Specification BS-2010A BS-2010B BS-2010C BS-2010D BS-2010E
Monocular Head,
inclined at 45° ●●●●
○
Viewing
Head
Sliding Binocular
Head,Inclined at ○○○○
●

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45°
Wide Field
Eyepiece
WF10X
●●●●●
Eyepiece
WF5X,P16X ○○○○○
Eyepiece
Eyepiece WF10X
with Pointer ○○○○○
4X,10X ,40X(S) ●●●●●
20X,60X ○○○○○
Objective
(Achromatic)
100X ○○○○●
Single Lens NA
0.65 with Disc
Diaphragm
●○○○○
Single Lens NA
0.65 with Iris
Diaphragm
○●○○○
Abbe NA=1.2with
Iris
Diaphragm,Spiral
Adjustment
○○●○○
Condenser
Abbe NA=1.2with
Iris
Diaphragm,Rack
and Pinion
Adjustment
○○○●●
Triple Nosepiece ●●●●○ Nosepiece
Quadruple
Nosepiece ○○○○●
Plain Stage with
Slide Clips 110×
120mm
●●○○○
Attachable
Mechanical Stage
110 × 120mm / 60 ×
30mm
○○●○○
Stage
Double Layers
Mechanical
Stage
125 × 115mm /
70
× 20mm
○○○●●

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Incandescent Lamp
15W
Brightness
Adjustment
●●●●○
Illumination
LED Lamp 1W
Brightness
Adjustment
○○○○●
Mirror ○○○○○
Focusingg
Systerm
Caaxial Coarse and
Fine
Adjustment,Movin
g Range 10mm
●●●●●
Green ○○●●●Filter
Blue ○○●●●
Note: ●Standard Outfit, ○Optional
4 Configuration
a) Eyepiece, Objective and Nosepiece
The monocular microscope has wide field eyepiece 10X and achromatic objective
4X, 10X, 40X and 100X. The nosepiece is small structured, and located stably and
exactly. It has a large and sharp image, with 360º rotatable monocular or sliding
binocular head.
b) Bend arm and Coarse and fine focusing knob
The curved arm use C-shaped ,has sprightly lines.Also, with upper focus
stop.the coarse focusing adjustment adopts dovetail guide which make focusing
adjustment smooth and comfortable.
c) Base：
The base is designed to the instrument, which having a streamlined shaped
circular,and it harmonized the aim’s shape slinky，Electrical components and
lamp built inside it as the illuminator.
d) Stage：
Fixed stage or moveable stage
e) Illumination
The system consists of illuminator which contains collector / mirror, iris aperture
diaphragm and condenser. And the condenser can be lift up and down.
5 Assembly
a) Working environment requirement：
1) Room temperature: 0-40℃.
Maximum comparatively humidity: 85%.
2）High temperature will result in mildew, dew and even ruinous instrument.

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3) Avoid from the dust room. When it is not in use, please cover the
microscope with dust cover.
4）Please place the microscope in a stable situation without any
surging.
b) Check the Input voltage: be sure the power supply voltage is accordant with the
nominal input voltage which is signed outside the microscope, or it will bring a serious
damage to the microscope.
c) Lamp
1. The lamp has been well adjusted and checked before the microscope
leaves factory.
2 .The lamp housing parts become extremely hot during operation. Take care
not to burn yourself.
3. Lamp replacement：The lamp has its standard service life. When it has
expired, a lamp replacement is necessary.set the main switch to off state,
disconnect the power cord from the wall outlet, then allow the old bulb to
cool before replacing the bulb with a new of the designated type .Open the
lamp holder on the bottom of microscope to do the replacement, the surface
of the new lamp bulb should be clean and free of fingerprint or dirt, which will
decrease the brightness or even explode the bulb .
Note: Must not missing the grounded screw
Donot touch the lamp`s glass part with your hands,when sitting shoud
take gloves or cover the lamp with protector. Fingerprint or dirt on the
surface of the lamp should be cleaned with the clean cloth with dipping
alcohol,which will decrease the brightness or even explode the bulb.
d) Eyepiece：
Please insert the eyepieces into the eyepiece tubes.(There is small screw in
the eyepiece ,which can make the eyepiece can not be pull out arbitrarily
when be screwed down.
e) Objective：
Lower the stage to its lower limit, then screw down the objectives into the
nosepiece (from low to high).
f) Condenser：
he condenser has been adjusted well before delivery.
g) Collector：
The collector has been adjusted well before delivery. Frame of the
collector`s pedestal is screw thread can taken by contrarotate
Note：It cannot be taken down when the outfit is LED.
h) Color filter：
Lay the filter into the iris diaphragm bracket under the condenser if
necessary.
6 Operation
1、Insert the eyepiece into the inclined eyepiece tube, then rotate the objectives

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in sequence, according to their magnifications, into the threaded holes of the
nosepiece. Place the specimen in the center of the field of view, first use low
power objective to find the specimen image and then observe it with high
power objective, at the same time, adjust fine focusing knob until the image is
clear. When using 100X oil-immersion objective, the space between the front
of objective and specimen surface should be full of cedar oil. Drops of cedar
oil must be strictly free from air bubble, and the objective should be cleaned
immediately after using, otherwise it may solidify and make cleaning
difficult.
2、In order to get a sharp and clear image, you must adjust the illumination
system. When using objectives of different magnification, please adjust the
iris diaphragm of the condenser to bring its numerical aperture and objective’s
numerical aperture into coincidence. Generally speaking, the rate of objective
increase，the location of the condenser need to move up. Aperture is design for
adjust NA and not for adjust brightness. Usually, aperture is open to 70~80% of
the objective’s exit pupil, until can see clearly image in the view.
3、When to watch the same type of specimens, can adjust limit screw to proper
position and lock it up with nut. Then directly make coarse focusing knob up to the
position where the first speciment can focusing clearly. That can simplify the
operation and reduce the working intensity.
Note: The same type of specimen refer to the thickness of the slice 、the coverslip
and the carry slip are uniform.
7 Maintence and storage
1、When you open the carton, please be careful not to make the lens dropping
2、All the lens has been adjusted by manufacturer already, please do not
disassemble by yourself.
3、Nosepiece, coarse and fine focus is installed precisely, please do not
disassemble by yourself.
4、You should make the instrument clean, and often wipe the dusts.
5、Place the instrument in a shady, cool and dry place, when finishing the
operation, always use the dust cover for protection.
8 Troubleshooting Guide
If problems occur during use, please review the following list and take remedial
action as needed. If you cannot solve the problem after checking the entire list,
please contact the service department for assistance.
1、Optical Part

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Problem Cause Solution
The nosepiece is not in he located
position(objective is
not in the center of
the optical path)
Turn to the right
position(Turn objective
to the optical light
center)）
The image of the filament is not
in the center Center it
the edge of the field of
view has shadow or the
brightnessis uneven
The surface of the lens is moldy
or has contaminant (
including condenser,
objective, eyepiece and
collector)
Clean it up
There are stains on the lens
(include
condenser,objective, eyepiece and
collector)
Clean it up
There are stains on the specimen Clean it up
Find dust and stain in
the field of view
The position of the condenser is too
low Loosen the condenser’s locking bolt
adjust the
condenser to the
right position, then
screw down.
There is no coverslip on the
specimen add coverslip
The coverslip is too thick or too thin Use the standard
coverslip
The specimen is placed inversely Reverse it back
There was oil on the dry
objective(easily happened in 40X
objective)
Clean it up
the image is
defocus(low
resolution/contrast) There are stains on the lens
（including condenser, objective,
eyepiece and collector）
Clean it up
The oil objective didn’t immerse
oil Use immerse oil
There was bubbles in the oil Eliminate the bubbles
Use a unsuitable oil Change to the
specified one
The size of the aperture
diaphragm is too big Minify it

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There are stains on the incident
lens of the binocular tube Clean it up
The size of the aperture
diaphragm is too small Open it up
The position of the condenser is
too low Adjust the position
The condenser is not in the
center of the field of view\the
condenser inclines
Install the condenser
again and adjust the
center carefully by the
centering bolt
The nosepiece is not in the right
position Turning it until it reach the “clicked” position
One side of the image
is dark
The specimen is floating Fix it
The specimen slips on the stage Fix itThe image shifts
during focusing The nosepiece is not in the right
position Turn it to the “ clicked ”position
The image is a little
yellow Not use the blue color filter Use the blue filter
The size of the aperture
diaphragm is too small Adjust again
The position of the condenser is
too low Adjust the position
The brightness is not
enough
There are stains on the lens
（include condenser, objective,
eyepiece and collector）
Clean it up
2、Mechanical Part
Problem Cause Solution
The image can not
focus when using
high magnification
objective
The specimen is placed
inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip
（0.17 mm）
The objective and the
specimen touch
when change the low
magnification to the
higher magnification
The specimen is placed
inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip
（0.17 mm）
The specimen is
not easy to move The specimen holder is not fixed Fix it
The eyes is too tired No diopter adjustment Adjust the diopter correctly
The brightness is not
suitable Adjust the voltage of the lamp

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3、Electrical Part
Problem Cause Solution
No power Check the connection of the
power cord
The bulb is not inserted Insert it correctly
The lamp can’t light
when turning on the
switch The bulb burns out Replace it
The lamp burns out
suddenly Use an unstandard lamp The voltage is too high
Use the specified lamp to
replace, if the problem is not
solved, contact with the
service department
Use a substandard lamp Use the specified lampThe brightness is not
enough The voltage is too low Add the voltage
The bulb flickers or
the brightness is
vertiginous
The bulb is going to burn
out Replace it