Biobase BSC-4FA2-GL User manual

Biosafety Cabinet Class II A2
BSC-4FA2-GL/BSC-6FA2-GL
User Manual
BIOBASE GROUP
Version 2021.06

1
Preface
Thank you very much for purchasing our class II A2 Biosafety Cabinet.
Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper
operation. After reading these documents, be sure to store them securely together with the “Warranty”
at a hand place for future reference.
Warning: Before operating the unit, be sure to read carefully and fully understand important
warnings in the operating instructions.

Content
Preface...................................................................................................................................................1
Content..................................................................................................................................................2
1.Unpacking,Installation,Debugging.................................................................................................... 4
1.1 Unpacking................................................................................................................................ 4
1.2 Accessories checking............................................................................................................... 6
1.3 Installation conditions and using environment........................................................................ 7
1.4 Installation................................................................................................................................7
1.5 Checking after installation..................................................................................................... 11
1.6 On-site debugging..................................................................................................................12
1.7 Test and standard................................................................................................................... 12
2. User Instructions............................................................................................................................. 14
2.1 Product instruction................................................................................................................. 14
2.1.1 Product concept............................................................................................................14
2.1.2 Application Range....................................................................................................... 14
2.1.3 Air filtration system..................................................................................................... 14
2.1.4 User instructions.......................................................................................................... 14
2.1.5 Circuit of digital control...............................................................................................15
2.1.6 Airflow velocity indication.......................................................................................... 15
2.1.7 Differential pressure indication....................................................................................15
2.1.8 Using of UV lamp........................................................................................................16
2.1.9 Using of socket............................................................................................................ 16
2.1.10 Installation methods of connect to the external exhaust system................................ 16
2.2 Structural composition........................................................................................................... 18
2.3 Control panel..........................................................................................................................18
2.4 The Usage of Remote Control............................................................................................... 20
2.5 Methods and procedures for disinfection...............................................................................22
2.5.1 Operation......................................................................................................................22
2.5.2 Structural material maintenance.................................................................................. 22

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2.5.3 Clean process............................................................................................................... 22
2.5.4 Vent tank......................................................................................................................22
2.5.5 Necessary disinfection................................................................................................. 23
2.6 Replacement part list..............................................................................................................23
2.7 Wiring diagram...................................................................................................................... 25
3．Common faults & solution............................................................................................................26
3.1 Common faults & solution.....................................................................................................26
3.1.1 Warning and reminder................................................................................................. 26
3.1.2 Trouble shooting.......................................................................................................... 26
3.1.3 Simple accessories replacement...................................................................................27
3.2 Label Description...................................................................................................................28
4. Warranty......................................................................................................................................... 30
Annex 1...............................................................................................................................................31

1.Unpacking,Installation,Debugging
Please firstly check if packing box is in good condition. If the packing box is damaged, please take
photos.
1.1 Unpacking
Choose the proper unpacking method according to the actual situation.
Method 1 Necessary tools for unpacking: Electric drill with hexagon dead M8
Picture 1
Method 2 Use M8 Wrench to unpack
Picture 2
Rapid unpacking diagram(Disassemble the screws shown in below Picture, then move the wooden
pieces to right and left)

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Picture 3
Notes:
1) Check the package after receiving the device. Broken glass or other visible damage must be
indicated in the equipment receipt, and immediately notify the shipper.
Note: Equipment cannot be returned to the factory, unless receive return authorization!
2) If the device is transported in cold weather, please place it in the area for 24 hours at room
temperature before putting it in working place. Please move the equipment as close as possible to
where you use it before dismantling packing.
3) Firstly, remove all surface protection. Before the entire device is exposed, do not try to lift off the
packing bracket, and make sure you have read the article as below.
4) Unpack the entire package; put away all components for assembly carefully. Left adhesive tape
can be wiped with alcohol.
5) Check all parts. If there is damage, please notify the transport company immediately. If the
device has problem, do not open the package, because shipper may need to check boxes.
6) Move the equipment off from collet, loosen locking block on the four corners.
7) Safety cabinet is very heavy and is equipped with HEPA filter. They should be transported lightly,
It is better to use big trolley to move the safety cabinet and don’t focus the whole weight of safety
cabinet at the middle of the work place.
8) Sometimes we need to raise the edge of the cabinet to enter some area, but this is only allowed of
a short distance.
9)It is forbidden to make it top-for-bottom or dumping while transporting or placing the Biosafety
Cabinet.

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1.2 Accessories checking
Refer to the packing list and check the accessories and materials
BSC-4FA2-GL/ BSC-6FA2-GL Packing list
Items
Model
Position
Quantity
Main Body
Wooden box
1unit
Base Stand
Placed in two carton box
1set
UV Lamp (T6 30W)
BSC-4FA2-GL
Top or back of main
body
1pc
UV Lamp (T6 40W)
BSC-6FA2-GL
1pc
Protective tube(12.5A)
Placed in a transparent
plastic bag
2pcs
Protective tube(6.3A)
3pcs
Protective tube(2.5A)
2pcs
Protective tube(125Ma,110V)/Protective
tube(63mA,220V)
1pc
Remote control (including battery)
1pc
User manual
1pc
Test report
1pc
Quality certification card
1pc
Drain Valve
1set
Stainless steel hex cylinder head bolt
M10×20
5pcs
Stainless steel flat washer10
5pcs
Stainless steel spring washer 10
5pcs
Inner hexagon wrench
1pc
Big rubber gasket
1pc
Small rubber gasket
1pc
Front window tubular motor control rod
1pc

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1.3 Installation conditions and using environment
To avoid disturbances to the safety cabinet and its operator, follow the following guidelines, while
determining a suitable location for the cabinet:
a. The distance from the plane of the aperture to any circulation space should be at least 1000 mm,
so as to preserve a zone undisturbed by anyone other than the operator.
b. Biosafety Cabinets should be placed in a position where there should be no opposing wall (or
other obstruction likely to affect the airflow) within 2000 mm of the front aperture.
c. Safety cabinets should not be installed in positions where they are likely to be affected by other
items or equipment. In particular the distance to the aperture of an opposing safety cabinet, fume
cupboard, or the edge of a local exhaust ventilation outlet should not be less than 3000 mm.
d. Any room air supply diffuser should not be within 1500 mm of the front aperture.
e. Doorways should not be within 1500 mm of the aperture or within 1000 mm of the side of the
safety cabinet.
f. The position of a safety cabinet should satisfy the spatial requirements (e.g. vision, lighting and
convenience of access) of the operator and personnel working nearby. When a cabinet is
installed on a bench top, the leading edge should be flush with or slightly overhanging the edge
of the bench top.
Working environment:
(1) Only is suitable for indoor;
(2) Ambient temperature: 15℃～35℃;
(3) Relative Humidity:≤75%;
(4) Atmospheric pressure range: 70 kPa～106 kPa;
(5) Electrical parameters: Consistent with the rated voltage of the Biosafety Cabinet (See 2.1.11
technical parameter performance index);
(6) Power supply need to be grounded; (Judging method: testing the fire wire and the zero line of
the power supply with multimeter, the fire wire to ground voltage should be grid voltage and the
zero line to ground voltage should be 0, otherwise the power supply ground is bad).
(7) Test the voltage stability before using, if the voltage is unstable, should use the voltage regulator,
otherwise the control panel and transformer may be easily damaged.
1.4 Installation
1) Install the base stand.
2) Put upperbody on the base stand and fix it with bolts which are packed in the bag in the work
area.Connect the base stand with cabinet.
3) Adjust foot caster and work surface, make sure the maximum stability on the uneven ground,
clockwise the red part of foot, lower the corresponding supporting feet, lower the height of
cabinet, lower four feet at the same time can move the cabinet; rotate the red part of the foot,
higher the corresponding base supporting feet, higher the height of cabinet, higher four legs at
the same time can make the cabinet at horizontal steady state.
4) Remove the internal packaging materials; empty all of the pieces, etc.
Note: any leaving fragments may be damage to fan and HEPA filters.
5) Check whether the accessories according to the product packing list.
6) Check is there any damage in the process of transportation and check the situation of all

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components. If necessary, test filter and the fixed bolt on the front panel, these bolts should not
be too tight. If you need to check the surface of the air filter, you can loosen bolts fixed well
wind network and take it out carefully from the front.
7) Check if the work place stable.
8) If the safety cabinet is equipped with water tap and gas tap, on the side wall is 1/2 inch threaded
pipe joint; Connected to the building or other connection should fit this size.
9) Install HEPA exhaust protective device on the exhaust HEPA filters: put the protective device
on the opening place of the filter and fix it with nut.
Note: do not put hand into the exhaust air filter at any case.
10) Fix drain valve on the bottom floor, connect the drain valve, and check the drain valve in the
closed position - in parallel with the ground position, as shown in figure 7.
11) The top of the device is equipped with HEPA exhaust protective device, if equipment requires
there is an external exhaust system (applied to the poisonous and harmful gas or steam).
Note: there is at least 300 mm clean space between the lab ceiling and the exhaust HEPA filter. If
the exhaust HEPA filter is blocked, there will be no enough air flow into the operation area.When
the exhaust HEPA filter at the top of the clean room is less than 406 mm, you must test the wind
speed at spot.
12) Install UV lamp.
13) Installation of the main components in Picture 4.5.6.
a. Installation of base stand ，Refer to picture 4 install base.
Picture4
1.Right bracket
2.Back connection plate, use six inside hex bolts M10 x 20 to fix with right and left bracket
3. Inside hex bolts M10 x 20, six pcs
4. Left bracket
b. Connect main body and base

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Picture 5
Aligned the mounting holes on the bottom of the cabinet side with the locating bolts, slow down the
cabinet on the mounting base.
Picture 6

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Take out the M10*20 Hexagon socket head screws, Flat washer 10, Spring washer 10 from the
accessory box, and fasten tightly according to Picture 6.
c. Installation of Drain valve
Picture 7
1. Drain valve connector
2. Shim
3. Safety cabinet bottom installation holes
4. Ball coupling fastening nut
5. Rubber gasket
6. Drain valve
7. Copper joints
d.Adjustment of Footmaster Caster
Picture 8
Clockwise rotate caster’s red part to low down the base feet and the height of the cabinet. Low
down all four casters can move the cabinet position. Counterclockwise rotate caster’s red part can
rise the base leg and height of cabinet. Raise all four casters at same time can fix the cabinet. Adjust
the four Foot -masters makes the cabinet stable.
e. Installation of water tap, gas tap

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Picture 9
1. Tighten nut 2.The tap/gas tap
Pick up the water tap, gas tap and tightening nut, install as shown in Picture 9.
1.5 Checking after installation
Checking Items
Normal situation
Wind speed display
Inflow 0.53m/s, downflow 0.33m/s
Pressure display
exhaust filter80-120Pa, downflow filter 80-120Pa
Fan operation
The fan running
Fluorescent lamp
Lamp lights after pressing button
UV Lamp
Lamp lights after pressing button
Display screen buttons
All buttons can be used
Socket
Press the socket key, multimeter testing output
supply voltage
1) Connect the Biosafety Cabinet dedicated power line to power outlet on the ground, electric
current is 9A, Biosafety Cabinet must be the only equipment on the circuit to ensure that does not
exist in any other equipment, see the electrical equipment requirements data in the cover.
2) Open the Fan key on Control Panel; fans will take some delay in order to achieve the required
speed. And in order to prevent too much current, the fans need a delay of about 10-15S in order to
achieve full speed before motor running. Similarly, the fans also need 10-15S delay to stop. Stable
voltage is provided by built-in voltage regulator circuit for the motor / blower, thus eliminate air
fluctuations.
Note: Please stop the fan before shut off power.

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3) Press the LIGHT button on control panel, turn on the lights. If the lamp is not bright, please
check lamp-tube or socket, it may course loose between the lamp and lamp holder in the carrying
process.
4) Press UV button on the control panel; turn on the UV lamp and to check whether UV light is
working properly.
Note: Only when the glass door is closed, UV can work.
5) Press key SOCKET button on the Control Panel; check the safety cabinet’s internal power socket.
If the safety cabinet equipped with two sockets, button controls both of them.
6) Press the mute button on the control panel to start the sound attenuation function, press again to
restore alarm.
7) Continue to press door up buttons on the control panel, glass door will continue rising, automatic
stop when up to10 inch, press this button again and the glass door will continues rising.
8) Continue to press door down buttons on the control panel, glass door will continue dropping,
automatic stop when down to10inch, press this button again and the glass door will continue
dropping.
1.6 On-site debugging
Before packing and transport, Biosafety Cabinet has been tested and confirmed at our factories, but
another test must be adopted. Only qualified personnel can adjust device, in order to ensure the
accuracy of the on-site testing, test equipment must be calibrated. If you can not implement this
operation, please contact the manufacturer for you to provide local on-site testing services company
names.
On-site debugging should include at least the following:
-Application of aerosol detection technology, check exhaust HEPA filters need a aerosol generator
and calibration photometer;
- Check the safety cabinet’s inflow while working;
- Check the safety cabinet internal decline airflow rate;
- Check airflow inside the cabinet by using smoke simulator;
- When the cabinet connected to building exhaust system, balance the air emission.
The safety committee to ensure that your new equipment accurate on-site inspection, the
equipment cannot be used without the pretest taken by safety committee.
Do not adjust the fan speed in the absence of fully testing of decline wind speed and the inflow air.
If the manufacturer has already set the correct air flow, generally do not require further adjustments.
Do not change the valve set of the ratio between the exhaust and air supply, unless qualified
personnel think it necessary to do it.
1.7 Test and standard
1) Filter leakage test
The standard aerosol testing tests the integrity of the HEPA filters, generally tested annually.
Qualified personnel must use calibrated device when aerosol testing. Ref to the list provided by
factory. Some associates have specific requirements about the aerosol test.
2) Air adjustment of the opening of the work area
The exhaust HEPA filter should be installed on the top of the Biosafety Cabinet. The air volume
exhausted is equal to the air volume entering from the working opening. The average velocity

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entering the opening of the work area has been identified when designs the Biosafety Cabinet. The
test should be conformed the rules of the NSF49. The opening air velocity testing data see the
APPENDIX 1.
To adjust the fan speed adjustment should be according to the data specified in the APPENDIX 1.
To set the air velocity of the opening work area correctly.
We emphasis again that only the qualified people can use the calibrated equipment to carry out the
adjustments. If there is some difficulties PLS contact the manufacturer.
Note: the adjustments must be made in conjunction with the down flow air velocity profiling section
3. And it is necessary to have a balance test between them.
3) Downflow air velocity:
The equipment should be balanced by it. And generally only the adjustment outlined in section 1.7
required. However when the HEPA filter blocked for more accumulations the readings on the static
pressure monitor increased (located on the control panel). The fan speed needs to adjust so that can
rebuild the correct air velocity within the working chamber. The equipment designed the air split.
And we can control the exhaust and inflow air ratio by adjusting the positions of the grids in the air
split. The setting is done in the factory and generally need no adjustment. The equipment correct
settings and the testing data see the APPENDIX 1.
NOTE: The adjustment of the balance of the air velocity must be carried out by the professional
person.

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2. User Instructions
2.1 Product instruction
2.1.1 Product concept
Biosafety Cabinet meet the standards of NSF/ANSI49-2012. However, the A/B3 is the old version.
The equipment has the following three functions:
-Protect the operators against the hazardous particles or hazardous particles in the air.
-Provide the clean working area, prevent the air pollutants from touching the working bench.
-Prevent the cross-infection of the samples on the sides.
2.1.2 Application Range
Biosafety Cabinet is necessary equipment in the laboratory in the search of microbiology,
biomedical, DNA recombinant, animal experiment, and biological products, especially in the
occasion that operator need to adopt protective measure, such as medical and health, pharmacy,
medical research. Our equipment provides a safety working environment which don’ t have bacterial
and dust in the process of bacterial culture.
2.1.3 Air filtration system
Picture 10 is the air flow pattern and protected area. The air filtration system is the most important
system, which consists of blower, air duct, supply filter and exhaust filter.
The function of air filtration system is transferring filtered air to work area, ensure the average down
flow velocity is 0.33 ±0.025 m/s, meanwhile, it can purify the exhaust to prevent environmental
pollution.
Picture 10
2.1.4 User instructions
(a) This equipment can work continuously, but in order to extend HEPA’s life time, we can open
only when need. Power on the equipment for several minutes before using, to ensure the steady flow
in the cabinet. If the cabinet is placed in the polluted area and no running, we should close the front
window. However, don’t operate the cabinet when the front window is closed.
(b) Using the disinfector wipe the inner of the work area, before running the equipment.
(c) When open the fan, read the pressure degree.
(d) Check drain valve and make sure it’s off.
(e) Arrange your experiment process carefully, don’t mess the work area.
(f) When the cabinet is running, it can storage clean instruments, but not too much.
(g) When sampling operation is finished, the samples should be moved to the left or right of the

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sampling dish. Correct operation can make sure there is no cross infection of the samples in the
cabinet.
(h) Note: The operator’s arm and hand maybe the pollution source, so the operator must wear
sterilized gloves or wash hands using sterilization soap. The used gloves should be gotten off from
the sleeve of lab-gown.
(i) When the room air or adjacent working area air is serious polluted, we should take measures to
prevent the particle from entering into the clean working area.
(j) The most basic is placing the instruments to the right place. If don’t know the place, using
airflow meter to check, to make sure the right airflow pattern.
(k) Don’t block the air grill, especially after we used the sterile rag.
2.1.5 Circuit of digital control
(a) Our Biosafety Cabinet use the latest microprocessor control technology to monitor your safety
cabinet’s function, easy to operate, with friendly interface and automatic diagnostic function.
(b) Connect safety cabinet to the recommended power interface.
(c) After power on, the LCD display will display the cabinet’s working time, and filter’s working
time and the life time. When push the power button, the LCD display background lights up, the
system will be in standby mode.
(d) After power on, the equipment will check itself automatically. When the front window is too
high, it will alarm buzzer, and the LCD display will show “!” and flicker, now you can adjust the
height of the front window. If the differential pressure of the supply filter and exhaust filter are too
big, there will be audio and visual alarm, then we need to replace the filter, to ensure the safety of
operator.
(e) After self-examination, the equipment will be in standby state.
(f) The fan run by activating the switch on the control panel, the Fan sign on the LCD display will
light, meanwhile, the motor will start, and it will reach the normal speed after 10-15 seconds.
(g) When activate the fan switch again, the motor and the sign of fan will close, and the motor will
shut down after 10-15 seconds.
(h) Three-pin socket is controlled by the relevant key on the control panel, the max current is 5A. If
there are two three-pin sockets, they will be controlled at the same time.
(i) The digital controller can adjust all velocity of the fan, and it can provide the linear compensation
for voltage fluctuation.
(j) The airflow volume will be displayed and monitored through the pressure, and will be sampled
by the microprocessor. When the airflow volume exceed the normal value, the buzzer will alarm
continuously.
(k) Before seeking the maintenance service help, pull the plug off the wall, then plug in to restart the
motor after about 2 minutes, the safety cabinet will restart and run well. But if the cabinet can’t start,
then seek the service.
2.1.6 Airflow velocity indication.
Airflow velocity indication is inflow velocity and down flow velocity.
2.1.7 Differential pressure indication
Differential pressure indication is the differential pressure between upper and lower filter, and it will
increase when the filter loading increase. The value after the Exhaust filter is the exhaust filter
differential pressure, the value after the downflow filter is the supply filter differential pressure.

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2.1.8 Using of UV lamp
Using UV lamp to sterilize the equipment for at least half an hour before and after use. When
sterilizing, the operator had better leave the room to protect eyes and skin.
2.1.9 Using of socket
The switch of socket is on the control panel, on the circuit board there is a 6.3A fuse for overload
protection. The power supply provided by the socket is 110V, 60Hz or 220V, 50Hz/60Hz, and the
maximum current is 5A.
2.1.10 Installation methods of connect to the external exhaust system
About the installation of Biosafety Cabinet exhaust HEPA filter connected to an external exhaust
system, we supply the following Suggestions.
When the Biosafety Cabinet is connected to the efflux system, it must be 100% efflux system.
1)Top pipe connection
(a) Typical installation is shown in figure 11.
Whether the safety cabinet is working, the exhaust hood can always provide continuous stable gas
flow exhausting outdoor. The total exhaust air rate of the exhaust hood is no less than 100m3/h of
safety cabinet’s gas displacement, gas displacement of safety cabinet BSC-4FA2-GL,BSC-6FA2-GL
are separately 590m3/h and 825m3/h.
(b)When the safety cabinet vent to a public exhaust system, this connection method is also
available.
(c)To make sure the disordered airflow inner side dose not leak to air outside; air curtain should be
used to check the unclosed roof.
(d) Ensure that there is no air exhaust fan working in the distance when the cabinet working. Air
exhaust fans on the building should be locked to cabinet’s supplier, or there should install a switch
or similar device on the laboratory’s exhaust system.
(e)To set the suction on the top correctly, there need an air throttle on the gas exhaust pipeline.
(f)Adjust the air flow balance by qualified person.
We can provide exhaust controller for connection the outside pipe. Each exhausting control
combination need an alarm monitoring system, including low flow alarm and high flow alarm.
Picture 11

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2) Hard connection belongs to A2 safety cabinet’s special operation method. If you need to use this
method, please contact us.
3)Flow alarm system instruction
Flow alarm system is an independent software package programmed in advance to monitor the
abnormal of air volume. It alarms when the air volume exceed normal value.
2.1.11Technical Parameter
Model
Technical parameter
BSC-4FA2-GL
BSC-6FA2- GL
Rated voltage AC
220V±10% □ 110V±10% □
Rated frequency
50 Hz □ 60Hz □
External Size(W*D*H)
1383×775×2295mm
1873×775×2295 mm
Working Zone
Size(W*D*H)
1210×600×660mm
1700×600×660 mm
Rated current
9A
9A
Total Airflow Volume
590m3/h
825m3/h
UV Lamp Consumption
30W
40W
Fluorescent lamp
Consumption
18W*2
22W*2
Down flow Velocity
0.33m/s
Inflow Velocity
0.53m/s
HEPA Filter
99.999％(Diameter:0.3μm)
Noise
NSF 49≤61dB / EN 12469≤58dB
Notes: (1) Electric consumption power including power which operation area needs to load(Loading
no more than 500W)
(2) Our company reserves the right of product design changes, if there are any design change,
without prior notice.

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2.2 Structural composition
Picture 12
1.Air Outlet safety guard 5.Alarm rest plate
2.Control panel 6.UV lamp
3.LCD display 7. IV bar
4.Fluorescent lamp 8. Base stand
2.3 Control panel
Picture 13

1.UV lamp
14.Glass window up status
2.Fluorescent lamp
15. UV status
3.Blower
16.Socket status
4.Socket
17. Fluorescent lamp status
5.Glass window up
18. Blower status
6.Glass window down
19. Exhaust filter differential pressure
7.Mute
20. Inflow velocity
8.Power
21. Supply filter differential pressure
9.Alarm status
22. Downflow velocity
10.Mute status
23. Filter working time
11.Filter changing status
24. UV lamp working time
12.System working time
25. Reservation timing
13.Glass window down status
a) LCD Screen
The working status of the equipment and operation can be seen on the LCD screen.
b) Soft touch button
BSC’s main functions could be executed by touch-buttons. User can operate the BSC either by
pressing the buttons on control panel or using the remote control. There are totally 8 common button
on control panel.
: The power button: Master switch to control other function keys
: To control fluorescent lamp. When you press each time, the status of the fluorescent lamp
and the corresponding status counter on the LCD change at a time from bright into extinguish or the
opposite.
: To control UV lamp. When you press each time, the status of the UV lamp and the
corresponding status counter on the LCD change at a time from bright into extinguish or the opposite.
(It works only after front window, fluorescent lamp, blowerfully closed.)
: To control blower working status. When you press each time, the status of the blower and the
corresponding status counter on the LCD change at a time from bright into extinguish or the
opposite.(It will not work when front window is fully closed.)