Biobase BCI-I User manual

Chicken Isolator
BCI-I
User Manual
BIOBASE GROUP
Version 2020.07

1
Preface
Thank you very much for purchasing our Isolator.
Please read the “Operating Instructions” and “Warranty” before operating this unit to assure
proper operation. After reading these documents, be sure to store them securely together
with the “Warranty” within touch for future reference.
Warning: Before operating the unit, be sure to read carefully and fully understand
important warnings in the operating instructions.
Disclaimer
Biobase shall not be liable for any equipment failure or damage, or for any direct or indirect
damage that may occur during the use of the equipment.
1.Malfunction or damage due to violation of the instructions, precautions, and intended use
of this manual.
2.Malfunction or damage caused by repair or alteration of the other company.
3.Malfunction or damage caused by use instruments of other company at the same time .
4.Malfunction or damage caused by operating environment not corresponding to the
specified operating environment (power conditions, installation environment, etc).
5.Malfunction or damage caused by natural disasters such as earthquakes and floods.
6.Malfunction or damage caused by the company unaware of the movement or transfer
(transport) after installation.

1
Content
Content...................................................................................................................1
1. Introduction....................................................................................................... 2
2. Technical Parameters.........................................................................................3
3. Features..............................................................................................................4
4. Structure.............................................................................................................5
5. Control System.................................................................................................. 7
6. Installation and Operation...............................................................................10
7. Maintenance and Trouble shooting.................................................................12
1. Power failure................................................................................................... 12
2. Fan blower failure........................................................................................... 13
3. Fluorescent lamp failure..................................................................................13
8. Labels...............................................................................................................15
9. Warranty.......................................................................................................... 16
Appendix A Circuit Chart....................................................................................17
Appendix B Packing list......................................................................................18

2
1. Introduction
The experimental animal is used for scientific research, disease diagnosis, vaccine and
blood serum products, as well as any other medicine management.
The experimental animal is very important for scientific research, it is named as live reagent.
Sterile chicken and SPF chicken are experimental material for Virology, nutrition,
pathological study, they are indispensable for biological products production and inspection
quarantine.
It is crucial to ensure the biological safety for animal feeding, our stainless steel Chicken
Isolator (Positive pressure or negative pressure) is suitable for SPF level experimental
animals and it is laboratory machine which can protect the experimental animals,
environment and operator.
Working Environment
a) Can only be used inside room；
b) Ambient temperature：15~35℃；
c) Relative humidity：≤80%；
d) Air pressure：70KPa-106KPa.

3
2. Technical Parameters
Model
Parameters
BCI-I
External Size
(W*D*H)
2200*850*1800mm
Animal Activity Area
(W*D*H)
1600*750*820mm
Waste collection Area
(W*D*H)
1600*750*250mm
Rated Voltage
AC220±10%
Rated Frequency
50Hz±1Hz
Consumption
700W
Air change Rate
≥20 times/h
Airflow Velocity
0.1m/s~0.2m/s within the chicken living space
Falling Microbial
number
(pc/dish hour)
0
Noise
≤70dB
Filters
Primary filter，medium efficient filter, HEPA （H14）filter
Display
LED display: temperature, humidity, Temperature adjustable
（10-50C), fan, illumination and UV lamb
Standard accessories:
Pre-filter (2 pcs), HEPA filters(2 pcs), filter cotton, UV lamp(1
pc), heating lamp(2 pcs), fluorescent lamp(1 pc), high quality
butyl gloves (6 pairs), feeding and drinking system

4
3. Features
1. LED display: real-time animal activity area temperature and humidity.
2. Automatic temperature control system，there are 2 heating lamps installed to make sure
the inner chamber temperature within setting range. The operator can set temperature
according to requirement.
3. Unique UV sterilization function, it is very convenient. And the right amount of UV
radiation can raise chickens growth, promote chickens bone. The machine sterilization
can also proceed through reserved hole (Pic 1), hot steam sterilization through the hole
in left top of machine and spray disinfection through the hole in right side of pass box.
4. Complete 304 stainless steel material.
5. Leakproofness: the unique structure design, at the same time, seam crossing is sealed
by special glue, which can ensure no air leakage.
6. Transparent glass window, very convenient to observe the animal status.
7. Three types filters (Pic 2), Primary filter，medium efficient filter, HEPA （H14）filter,
ensure the both inner chamber and ambient environment cleanliness. The primary filter
is washable, it can keep big particles out, by which is effective to extend medium filter
and HEPA filter life.
8. Semi-auto waste collection and exhaust system, easy-to-clean design.

5
4. Structure
The Isolator is composed by upper body and down body. Easy assembly and
disassembly design.
Pic 1
1. Pass box; 2. Pressure gauge; 3. Control system box; 4. Waste collection membrane; 5.
Base stand; 6. Universal caster with brake; 7. UV lamp and fluorescent lamp
installation groove; 8. Hot steam sterilization hole ; 9. Depressurization type water tank;
10. Water inlet; 11. Blower; 12. Heating lamp –TWO; 13. Glove; 14. Drainage ball
valve; 15. Filters box; 16. Heating lamp-ONE; 17. Waste collection box; 18. Waste
outlet

6
Filter pattern
Pic 2

7
5. Control System
Pic 3
1. Fluorescent lamp; 2. UV lamp; 3.Blower; 4. High and low temperature alarm setting;
5. Heating lamp; 6. Humidity display; 7.Temperature display;
8. High temperature alarm indicator
9. Low temperature alarm indicator
5.1. Preparation
Connect power supply cord（220V±10%，50Hz）, turn on power button, then the display will
show inner chamber temperature and humidity.
5.2. Press “ ” button, the light indicator on, fluorescent lamp starts to work; press
“ ” button, the light indicator off and the fluorescent lamp close.
5.3. Press“ ” button, the UV indicator on, UV lamp starts to work; Press“ ” button
again, the UV indicator off and UV lamp close.
5.4. Fan blower

8
Press “ ” button, the fan blower indicator on, and blower starts to run, press “ ”
blower again, the fan blower indicator off and fan blower stops running.
Keep pressing “ ” button 5 seconds, the humidity display window (refer to pic 3, 6.
Humidity display) will show blower status (gear), press button to increase blower
speed and to reduce blower speed, the gears are 1 to 5. The required pressure is
100Pa-150Pa, please adjust fan blower to make the pressure gauge value within
100Pa-150Pa.
At last, press “ ” button to save the setting and out of setting mode.
5.5 Temperature setting
Press “ ” button, the heating indicator is on, heating lamp starts to work (if the
displayed temperature is within setting temperature range, the indication is not on); press
“ ” button again, the indicator off and heating lamp stops working.
Keep press “ ” button 5 seconds, set required highest temperature through the
temperature display window (refer to pic 3, 7. Temperature display)( press to
increase temperature and reduce temperature), then press “ ” button to save
data; set required lowest temperature through humidity display window(refer to pic 3, 6.
Humidity display)( press to increase temperature and reduce temperature),
then press “ ” button to save data and out of setting mode.
Note:
When the display temperature is within setting temperature range, the two heating lamps are
not on; when the display temperature is below the lowest setting temperature, both heating
lamps will heat; when the display temperature reaches at lowest setting temperature,
Heating lamp-TWO will work, Heating lamp-ONE does not work (refer to pic 1), if the
display temperature keeps reduce till 0.5 ℃below the lowest setting temperature, the
Heating lamp-ONE starts to work ; when the display temperature reaches at highest setting
temperature, both heating lamps don’t work.
5.6 Temperature Alarm Setting
Keep pressing“ ” button 5 seconds, then HIGHT indicator on, by now setting high

9
temperature alarm value through Humidity display window ( press ”、“ ”buttons
to modify the high temperature alarm value), then press “ ” button to save data and
following turn to low temperature alarm setting status （“HIGHT” indicator off, “LOW”
indicator on, by now setting low temperature alarm value through Humidity display window
( press ”、“ ”buttons to modify the low temperature alarm value), finally, press
“ ” button to save data, at the same time, “LOW” indicator off, out of setting mode,
and the humidity display will back to humidity displaying state.

10
6. Installation and Operation
(1). Installation
a) Remove package;
b) Check the machine and make sure no damage;
c) Count the accessories and files by referring to packing list;
d) Move the machine to installation place;
e) Take depressurization type water tank from Isolator inner chamber, adjust water
level control knob according to water using requirement, then have the cover on
and put water tank in the top of machine(refer to pic 1). Connect water tank with
machine through water feeding inlet (refer to pic 5).
(Note: Please prepare water pipe to connect water tank and water tap, the water pipe
diameter is 25mm)
Pic 4

11
Pic 5
(2). Operation
a) Connect power supply (220V±10%，50Hz), turn on power button, the machine is
now in stand-by status and the digital display shows inner chamber temperature
and humidity;
b) Turn on fan blower and adjust fan blower (refer to 5.4) to make the pressure gauge
between 100Pa-150Pa.
c) Normal goods delivery into inner chamber: open pass box outside door, put the
packaging items inside pass box and then close outside doors. Spray disinfectant
through the mouth in right side of pass box, after 20-30 minutes disinfection for
normal goods, then open inside door of pass box and take goods into inner chamber
and close inside door. Regarding for goods without package, but can undertake
disinfectant, please use the same disinfection way as packaging items and make
sure to clean goods before inside pass box.
d) Normal goods delivery out of inner chamber: open inside pass box door at first,
take goods into pass box and close inner pass box door. Spray disinfectant and keep
about 20-30 minutes for disinfection before taking the goods out. The infected
animal should be dead before taking out, the procedure is same as normal goods.
At last, please clean pass box and spray disinfectant for disinfection at least 20
minutes.
e) Waste delivery out of chamber: first, please connect waste outlet with autoclavable
disposal bag, then collect all the waste in waste box; open the inner door of waste
outlet, take waste out by gloves and cut waste collection membrane(by now, the
unused membrane replace used part), close inner door of waste outlet. (refer to
pic 1, 17 & 18)
f) Disinfection of waste: after collect all the waste inside disposal bag, please spray
disinfectant for disinfection at least 20 minutes and take disposal bag away from
waste outlet.

12
7. Maintenance and Trouble shooting
1. Application environment
Only professional staff is allowed to go inside isolator laboratory; the staff should wear
protection suit when working inside room.
No dusty materials are allowed in laboratory.
Please keep the laboratory wall, ground and isolator surface clean.
Please disinfect laboratory for a period of time;
Please keep laboratory air fresh;
2. Maintenance
Surface cleaning: Using soft cloth which is immersed in concentrated soap solution to wipe
the isolator surface, at the same time, prepared another piece of soft cloth which is
immersed in clean hot/warm water for cleaning isolator surface. At last, use dry soft cloth to
dry the surface.
Inner chamber cleaning: for your convenience, it is allowed to use Potassium Permanganate
solution (14g/m3) and Formaldehyde solution (28ml/m3) for sterilization. The sterilization
time is not less than 24 hours. After sterilization, the operator has to turn on fan blower
running for at least 24 hours to exhaust harmful gas.
Note:
Please make sure the Potassium Permanganate solution (14g/m3) and Formaldehyde
solution (28ml/m3) are not allowed for glass window sterilization, you can use Ammonia
disinfectant or washing detergent.
If the laboratory room temperature is less than 15 ℃, please turn on heating lamp to
facilitate sterilization procedure and improve sterilization.
Feeding system cleaning: Please clean food feeding and water feeding system each day, and
immerse the feeding system in TH14 disinfection solution ( 1:800) at least 10 minutes and
natural drying.
The primary and medium efficient filters washing time interval is according to environment
cleanliness, it is suggested to be 1 to 2 months. HEPA filter is suggested to be replaced each
12 months.
Trouble shootings
1. Power failure
Power failure means the machine cannot work after connect power cord and press power
button on. The solution is as below:
①Check if there is normal electricity supply and if it is required power voltage.

13
②Check if the power cord is broken or not by multimeter.
③Check the fuse. The fuse is located near power socket. If confirm the problems are not
caused by ①②, please check if the fuse is fusing breaking and replace by new one.
④Check if the relay is broken or wires is well connecting; please replace relay or well
connect the wires.
2. Fan blower failure
Fan blower failure means the blower doesn’t work after pressing FAN button, the solution is
as below:
①connect power cord and press power button, and next to press FAN button, then remove
the back panel of control system box. Detect the voltage of FAN button by multimeter, if
there is voltage reading in multimeter, the FAN button is broken and should be replaced by
a new one.
②If the FAN button is normal, connect fan blower to power supply directly, observe if the
fan blower works or not, if it doesn’t work, the fan blower is broken and should be replaced
by a new one.
③If confirm the problems are not caused by ①②,please inspect the wires connection of
buttons and make sure the wires are well connected.
3. Fluorescent lamp failure
Fluorescent lamp failure means the lamp doesn’t work after press Light button, the solution
is as below:
Check If the lamp is well connect with lamp holder.
Check if the lamp is broken, if the two sides turn black, the lamp is broken and should be
replaced by a new one.
Connect power cord and press power button, and next to press Light button, then remove
the back panel of control system box. Detect the voltage of Light button by multimeter, if
there is voltage reading in multimeter, the Light button is broken and should be replaced by
a new one.
4.Heating lamp-ONE failure
Heating lamp-ONE failure means heating lamp-ONE doesn’t work after pressing Heating
button, the solution is as below:
①Check the setting temperature range, if the display temperature is within setting
temperature range or higher than highest setting temperature, heating lamp-ONE should be
off;
②If the display temperature is below lowest setting temperature, the heating lamp-ONE
should be on, if not, please inspect if the heating lamp is fixed tight with lamp holder.

14
③If confirm the problems are not caused by ①②, then try to connect power cord, turn on
power button, and next to press Heating button, then remove the back panel of control
system box. Detect the voltage of Heating lamp-ONE by mulitimeter, if there is voltage
reading in multimeter, the heating lamp-ONE is broken and should be replaced by a new
one. If there is no voltage reading in multimeter, the circuit board is broken and should be
replaced by a new one.
5.Heating lamp-TWO failure
①Check the setting temperature range, if the display temperature is over the highest setting
temperature, the heating lamp-TWO should be off;
②If the problem is not caused by ①, then try to connect power cord, turn on power button,
and next to press Heating button, then remove the back panel of control system box. Detect
the voltage of Heating lamp-TWO by mulitimeter, if there is voltage reading in multimeter,
the heating lamp-TWO is broken and should be replaced by a new one. If there is no voltage
reading in multimeter, the circuit board is broken and should be replaced by a new one.

15
8. Labels
1. LOGO
2. Fuse
3. Ground

16
9. Warranty
1. Warranty period 12 months since the date of shipment, exclude consumables and
vulnerable parts (lamps, fuse, filters, glasses).
2. The warranty is responsible to factory defects, any man-made defects or nature force
majeure defects are not included.
3. Our company will keep maintenance service, but, reasonable maintenance fees will be
charged.

17
Appendix A Circuit Chart

18
Appendix B Packing list
Item
Quantity
Main body
1
RVV power cord
1
Food feeding bucket
1
Depressurization water tank
1
User Manual
1
Fuse（10A）
2