Bioneer Exicycler 384 User manual

Exicycler ™ 384
Real-Time Quantitative Thermal Block
35℃
15℃
MT Promedt Consulting GmbH Altenhofstr. 80
D-66386 St. Ingbert. Germany. Tel [phone]
A-2061

Exicycler ™ 384
Exicycler™ 384
Real-Time Quantitative Thermal Block
User’s Guide
Bioneer Corporation
8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon
34302, Republic of Korea
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.bioneer.com

Exicycler ™ 384
Intended Use
This device is used for the qualitative or quantitative analysis of specific DNA/RNA in samples.
Warranty and Liability
All Bioneer products undergo extensive Quality Control testing and validation. Bioneer guarantees quality
during the warranty period as specified, when following the appropriate protocol as supplied with the product.
It is the responsibility of the purchaser to determine the suitability of the product for its particular use. Liability
is conditional upon the customer providing full details of the problem to Bioneer within 30 days.
Trademark
Exicycler ™ is a trademark of Bioneer Corporation.
Copyright
Copyright 2018. Bioneer Corporation. All Rights Reserved
Notice
Bioneer corporation reserves the right to make corrections, modifications, improvements and other changes
to its products, services, specifications or product descriptions at any time without notice. All information
provided here is subject to change without notice.

Exicycler ™ 384

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Exicycler ™ 384
Contents
Before Starting ....................................................................................................................... 2
How to Use This Guide .................................................................................................. 3
Symbols on Exicycler ™ 384 ............................................................................................ 5
Safety Instructions .................................................................................................................. 6
Safety Warnings and Cautions ......................................................................................... 7
Introduction..........................................................................................................................10
Overview .................................................................................................................. 11
System Components and Specifications ......................................................................................12
Overview .................................................................................................................. 13
System Components ................................................................................................... 13
Specifications ........................................................................................................... 13
System Views............................................................................................................ 15
Installation and System Operation ..............................................................................................16
Overview .................................................................................................................. 17
Site Requirements ...................................................................................................... 17
Required Materials ...................................................................................................... 17
Unpacking Exicycler ™ 384............................................................................................ 17
Setting Up the Computer .............................................................................................. 18
Connecting the Computer and Exicycler ™ 384 ................................................................... 18
Installing the Operation and Analysis Program .................................................................... 19
Turning On and Self-diagnosis ...................................................................................... 31
Running Real-Time PCR Using Exicycler ™ 384 Software ...................................................... 32
Performance Maintenance........................................................................................................47
Performing System Diagnosis using ExiCfg ....................................................................... 48
Guide to AccuPower
® Fluorescence test kit for calibration...................................................... 50
Calibration using ExiCfg ............................................................................................... 52
Cleaning the main body ............................................................................................... 62

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Replacing Fuses ........................................................................................................ 63
Returning Exicycler ™ 384 for Service ............................................................................... 64
Troubleshooting.....................................................................................................................65
Ordering Information and Warranty .............................................................................................70

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Before Starting

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Exicycler ™ 384
How to Use This Guide
This guide is written for researchers and laboratory staff responsible for installing and maintaining the
Exicycler ™ 384 Real-Time Quantitative Thermal Block.
General Requirements for Installation
This guide assumes that you:
Are familiar with Microsoft®Windows®7 & 10 operating system.
Have basic techniques for handling DNA and RNA samples for PCR.
Have basic skills for data storage, copying, and pasting data into hard drives.
Have an experience in setting up a network. (Using any data generated by Exicycler ™ 384 requires
setting up a network.)
Word Conventions
Bold signifies user action such as typing a text or clicking a button. For example:
Type Test and click OK to move to the next step.
 Italic represents important words or sentences and is also used for emphasis. For example:
After analysis, you must save data using Save As.
A right arrow in bold (>) separates consecutive commands you select from a main menu or shortcut
menu. For example: File> Config> Scan
How to Obtain More Information
For more information about Exicycler ™ 384, please visit us online at www.bioneer.com.
How to Reach Customer Support
To obtain prompt customer support, please call us at [phone]. You can also obtain technical
support through www.bioneer.com (Email: exicycler-suppor[email protected]).
Product Use Limitations
 Exicycler ™ system is designed and sold for in vitro diagnostic (IVD) applications in combination with
the diagnostic kits manufactured by Bioneer and labeled for diagnostic purposes. Exicycler ™system
can also be used for life science research and other laboratory purposes.
Gradient function of Exicycler ™384 is not available in US, EU, Japan and Germany.
Use of Exicycler ™384 is covered by U.S. patents Nos. 8,139,210, 8,427,643, and their foreign
counterparts or pending patents. The purchase of these products includes a limited, non-transferable
immunity from suit under the forgoing patents for using only this amount of product solely for the
purchaser’s own internal research. No other patent rights to use this product for any other purpose or
for commercial purpose, including without limitation reporting the results of purchaser’s activities for a
fee or other commercial consideration, are conveyed expressly, by implication, or by estoppels.
Further information on purchasing licenses may be obtained by contacting Bioneer at
[email protected]m.

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Exicycler ™ 384
Safety Labels Description on Exicycler ™384
English
Hot surface.
Note that the surface is hot.
Francais
Surface chaude
Faites attention à la surface chaude.
English
Do not work the bath at low temperature for long hours.
This can cause condensation and lead to equipment malfunction.
Francais
Ne pas utiliser le bain à basses températures pendant plusieurs heures.
Risque de condensation pouvant entrainer des fonctionnements défectueux de
l’équipement.
English
Be aware of the door.
Please pay attention to the door when it is moving.
Francais
Manipulation de la porte.
Risque de blessures corporelles (pincement/écrasement) lors de.
l’ouverture/fermeture de la porte.
English
CRUSH HAZARD
Stay clear of this area when the door is in operation.
Unawareness can cause injury.
Francais
Risque d’écrasement
Ne pas rester proche de la porte durant son fonctionnement. Risque d’écrasement
de doigts en cas de contact avec la porte.

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Symbols on Exicycler ™384
Safety Symbols on Exicycler ™384
The following table describes the safety symbols that may be displayed on Exicycler ™384. Each symbol may
appear by itself or with text that explains the relevant hazard.
Hot surface.
Note that the surface is hot.
Do not work the bath at low temperature for long hours.
This can cause condensation and lead to equipment malfunction.
Be aware of the door.
Please pay attention to the door when it is moving.
CRUSH HAZARD
Stay clear of this area when the door is in operation.
Unawareness can cause injury.
Electrical Symbols on Exicycler ™384
The following table describes the electrical symbols that may be displayed on Exicycler ™384.
Indicates On position of the main power switch.
Indicates Off position of the main power switch.
Indicates a standby switch by which the instrument is switched on to the
Standby condition.
Indicates a protective grounding terminal in the instruments that must be
connected to earth ground.
Indicates a terminal that can receive or supply alternating current or voltage.
Indicates a terminal that can receive or supply direct current or voltage.
Environmental Symbols on Exicycler ™384
The following symbol (WEEE) applies to Exicycler ™384 placed on the European market.
Do not dispose of Exicycler ™384 as unsorted municipal waste.
Follow local municipal waste ordinances for proper disposal provisions to
reduce environmental impact of waste from electrical and electronic
instrument.
European Union customers:
Call your local European office for Bioneer instruments pick-up and
recycling.

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Exicycler ™ 384
Safety Instructions

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Safety Warnings and Cautions
Safety instructions in this guide book are to cover possible dangerous conditions and situations that may
occur. It is your responsibility to read this guide thoroughly when installing, maintaining, or operating
Exicycler ™ 384. The following safety alerts will be used in this section:
Hazards or dangerous actions that may result in severe injury.
Hazards or dangerous actions that may result in minor injury or damage.
Hazards or dangerous actions that may cause a burn.
Hazards or dangerous actions that may result in electric shock.
Location Consideration and Installation
General Instrument Safety
1) Check the power voltage rating before connecting to the instrument and an electrical outlet.
Exicycler ™ 384 is configured for 100-240VAC (±10%). Using AVR (Automatic Voltage Regulator) or
UPS (Uninterrupted Power Supply) is recommended.
2) Exicycler ™ 384 must be grounded for protection against electric shock. If not, it may cause serious
injury and system damage.
3) Do not place any objects in front of the main door of Exicycler ™ 384 that can interfere with door
operation.
4) Leave 50 cm space between Exicycler ™ 384 and the wall for proper ventilation.
5) Do not install Exicycler ™ 384 in a dusty environment to prevent false operation or technical damage.
6) Keep Exicycler ™ 384 away from heat sources.
7) Exicycler ™ 384 must not be installed in an area where it is exposed to water or is humid. It may
cause electric shock, a fire, or system damage.
8) Exicycler ™ 384 must not be installed in an area where it is exposed to combustible or flammable
vapor. In case of a gas leak, open windows and let fresh air in. Do not operate any electrical switch
during a gas leak. It may cause an explosion or fire.
9) Do not disassemble or repair Exicycler ™384 yourself. It may cause a fire, electric shock, and
system damage. A limited warranty does not cover unauthorized alterations or damage due to abuse.
Precautions regarding the electrical environment
1) If the power cord is loose, do not use the instrument. Overheated power cord may result in shock or
fire.
2) Do not operate multiple instruments out of a single wall outlet. The load may cause the instrument
to overheat and lead to fire.
3) When plugging or unplugging the power cord from a wall outlet, make sure your hands are
completely dry. Wet or moist hands may cause electric shock.
4) A convenient and safe power cord should be available. The power cord provided by our company
should be used.
5) Socket outlets should be at least 1.5 m from a sink or wash basin.

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Exicycler ™ 384
6) Power cord should not be repaired with insulating tape. Water can still penetrate insulating tape and
power cord.
7) If you use other power cord, it should be adequate for the electrical capacity for Exicycler ™ 384
(250V, 16A, 0.75mm2, VDE).
8) Plugs should match the socket outlets.
9) Unplug the power cord from Exicycler ™ 384 when not in operation for a long period of time to
prevent the possibility of fire by overheating.
10) Exicycler ™ 384 is equipped with a 3-conductor AC power cord that, when connected to an
appropriate AC power outlet, grounds (earths) the instrument. To preserve this protection feature, do
not operate the instrument from an AC power outlet that has no ground (earth) connection.
11) Improper fuse or high-voltage supply can damage the instrument wiring system and cause a fire.
Before turning on the instrument, verify that fuses are properly installed and that the instrument
voltage matches the power supply in your laboratory.
12) For continued protection against risk of fire, replace fuse types (250V, F10AL, 2ea) according to
rating specified for the instrument.
Installation Safety
1) Place and install Exicycler ™ 384 away from direct sunlight.
2) It is recommended that you turn off the computer and unplug the power cable before connecting it to
Exicycler ™ 384. If the computer is turned on while connecting, the communication port connector
either in the computer or Exicycler ™384 may get damaged.
3) Make sure that the USB cable is firmly connected to Exicycler ™384 and the computer. Unstable
connection may cause damage to the communication port connector or data transfer errors.
4) Built-in camera of Exicycler ™ 384 is a static-sensitive device. Pay particular attention to any cables
connected to Exicycler ™ 384 to avoid static damage.
Cautions
1) Ensure that the power cable is clean and connect it firmly to Exicycler ™ 384.
2) Operate Exicycler ™ 384 in a place where the temperature is always between 15℃ and 35℃. Poor
performance is influenced by extreme temperature. High temperature can cause mis-operation and
poor performance.
3) Operate the system in a place where humidity is always between 20 % and 80% with no
condensation. Highly humid environment can cause corrosion of internal components and low
humidity can lead to errors.
4) Do not place any objects behind or by the side of Exicycler ™ 384 that can interfere with ventilation
and cause errors.
5) The internal optical components of Exicycler ™ 384 may get damaged when the instrument falls or is
exposed to excessive physical shock.
6) Unplug the power cable from Exicycler ™ 384 when not in operation for a long period of time to
prevent the possibility of fire by overheating.

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Operation and Maintenance
Warnings
1) The system can be hazardous when misused.
2) Keep the 384-well thermal block area clean to prevent damage and to generate accurate
experimental data.
3) After any Real-Time PCR runs including ‘Scan’ or ‘Melting’, allow the light source lamp to cool down
for at least 10 minutes. Continuous operation without a break will reduce the lamp life span and
cause errors.
4) Do not place a piece of paper or a plastic cover under Exicycler ™ 384. It could cause a fire.
5) Do not turn off Exicycler ™ 384 right after a Real-Time PCR run is done. Wait until the cooling fan
has stopped running completely. Cooling fan remains running for about 2 minutes to cool down the
lamp after each Real-Time PCR run.
6) Do not cover Exicycler ™ 384 with a piece of paper or a plastic cover. This may cause a fire or
failure of the system.
7) Set ’Power Options Properties’in the Control Panel to ‘Turn off monitor’, ‘Turn off hard disks’,
‘System standby‘, and ‘System hibernates’ to ‘Never’. Otherwise, data transfer between Exicycler ™
384 and the computer will be interrupted.
IMPORTANT:
Exicycler ™ 384 should be operated in a clean condition. Contaminants such as dust can cause
problems and reduce the life span of Exicycler ™ 384. Please prevent dust from entering into Exicycler ™
384. In order to extend life span of Exicycler ™ 384, thoroughly remove dust periodically.

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Exicycler ™ 384
Introduction

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Exicycler ™ 384
Overview
Exicycler ™ 384 Real-Time Quantitative Thermal Block combines a thermal block in a special 384-well
format and is an innovative fluorescence detector. The detector monitors fluorescence emitted as an
indicator for amplified nucleic acid product during each PCR cycle in real time.
Thermal block is built into the lower part of Exicycler ™ 384, which carries out a thermal cycling. The
detector is located in the upper part of Exicycler ™ 384, which measures the fluorescence emitted from
samples in the thermal block in real time and transfers data to the computer for analysis. Data
transferred from the detector is analyzed with Exicycler ™384 analysis program.
Exicycler ™ 384 Real-Time Quantitative Thermal Block provides a maximum ramping rate of 4.5℃/sec,
and features various functions such as gradient, time increment, temperature increment, and ramp rate
control for myriad applications.
Exicycler ™ 384 Real-Time Quantitative Thermal Block detector consists of a light source and a
detector. The light source is a short arc lamp and emits a white-light as an energy source to excite
fluorescent dyes. A white-light source is divided into particular wavelength groups by band path filters.
The band path filters in Exicycler ™384 covers within 475 to 690 nm and works as a set consisting of an
excitation and an emission filter. Five filter sets are provided for various applications. Therefore, no
additional band path filters is required. Also, Bioneer’s imaging technique based on polarization of light
enhances detection sensitivity for robust and reliable results. Patented (KR10-1089045, US8427643)
polarizing optical apparatus mitigates the common problem of a reflection light, allowing precise
quantification and target discrimination.
The detector is a highly sensitive 2D CCD camera and detects mulfiple fluorescence signals from a
384-well plate at once. This simultaneous detection has a great advantage over sequential detection.
This innovative detector, invented by Bioneer, reduces well-to-well variation and has minimized dye-to-
dye interference, therefore providing more accurate results.
Bioneer’s state-of-the-art technologies applied to Exicycler ™ 384 generate a uniform light surface
over the thermal block. This allows detection of multiple fluorescent signals emitted from various dyes
through the light surface and separate signals within a selective wavelength range of each dye.
Exicycler ™ 384 Real-Time Quantitative Thermal Block features an auto loading function for automation
reducing errors and self-diagnosis for diagnosing systematical conditions of Exicycer ™ 384 for users
convenience. Exicycler ™ 384 software is composed of three main programs.
1) A set up program for fluorescence test, diagnosis and confirmation of the instrument’s information.
2) An operation program for creating a protocol, assigning a probe and plate, saving & displaying
data while operating Exicycer ™ 384.
3) Analysis software that comes with Exicycler ™ 384 has 4 different tools. The analysis program
includes Absolute Quantification, Relative Quantification, SNP genotyping, and Existentence / Non-
existentence. It is applicable to Gene expression, Quantification of cell and virus, and SNP
genotyping.

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System Components and Specifications

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Exicycler ™ 384
Overview
Exicycler ™ 384 Real-time Quantitative Thermal Block is warranted by Bioneer against manufacturing
defects in materials and workmanship for a limited warranty period of one year. Bioneer will charge for
repairing products for the following conditions:
Fault caused by the customer.
The product is repaired after expiry of the warranty period.
Before you install Exicycler ™ 384, check shipped materials with the system component listed below:
System Components
Component Lists
Check
Exicycler ™384
1
□
USB 2.0 high speed cable
1
□
Power cord1
1
□
Software
1 CD
□
User Guide
1
□
Optical tape sealing applicator
1
□
1Provided according to country standard.
Specifications
Physical specifications
Dimension
35.5cm(W) x 54cm(D) x 47cm(H)
13.98in(W) x 21.26in(D) x 18.50in(H)
Weight
41 ㎏(90.39lbs)
Sample capacity/ size
384-well plate
Sample volume
5~20 ㎕(10 ㎕recommended)
Power consumption
100-240VAC, 50/60㎐, Max 800VA
Operating temperature
15~35℃(59~95℉)
Operating humidity
20~80%, no condensation
Thermo module specifications
Method of heating / cooling
Peltier
Temperature range
4.0℃ ~ 99.9℃ (39.2~211.82℉)
Max ramp rate
4.5°C/sec (8.1℉/sec)

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Temperature accuracy
0.3℃ (±0.54℉)
Temperature uniformity
0.3℃(±0.54℉)
Lid temperature
40 ~ 120℃ (104~248℉)
Gradient range
20 ~ 95℃(36 ~ 203℉)
Temperature differential range
1 ~ 20℃ (1.8 ~ 36℉)
Temperature increment range
0.1~2.0℃(0.18~3.6℉)
Time increment range
1 sec ~ 60 sec
Ramp rate control range
1~100%
Computer specifications
Operating system
Window7 & 10(32/64 bit OS)
Processor speed
Intel Dual Core (1.8GHz) or higher
Memory
1GB or higher
Communication port
USB 2.0 high speed
Screen resolution
1280 x 1024 or higher
Optical Part
Light source
Short arc lamp(120W)*
Sensor
16 bit 2D CCD
Excitation Filter / Emission Filter**
5 Set
* Continuously turning on and off the lamp reduces the lamp life span.
* The lamp generates heat when it starts. Make sure that you turn off Exicycler ™ when cooling fan stops running.
It usually takes about 2 minutes for the cooling fan to stop.
**Please refer to a chart below for more information about filter sets.
※The specifications of this product may change without notification for performance enhancement.
† Filter sets
Position
Excitation (㎚)
Emission (㎚)
Set
Fluorescent dye
1
Blank
Blank
1
-
2
475
530
1
FAM, SYBR Green I
3
520
560
1
JOE, TET
4
550
590
1
TAMRA, CY3
5
570
630
1
Texas Red, ROX
6
630
690
1
CY5
Chemical hazard: Exposure to following florescent dyes may cause eye and skin and respiratory tract

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irritation. Read the MSDS before use and follow the instructions if swallowed or inhaled. Wear
appropriate protective eyewear, clothing, and gloves.
System Views