Bioneer ExiPrep K-3200-CB User manual

ExiPrep™
Beef
Genomic D
NA Kit
Cat. No. K-3200-CB
© Copyright 2022 BIONEER Corporation. All rights reserved.

ExiPrep™ Beef Genomic DNA Kit
Kit for the extraction of beef genomic DNA
User Gui e
K-3200-CB
96
Version No.: 4 (2022-06-10)
Please rea all the information in booklet before using the unit
BIONEER Corporation
Bioneer Global Center, 71, Techno-2-ro,
Yuseong-gu, Daejeon, 34013, Republic of Korea
Tel: [phone]
Email: [email protected]
www.bioneer.com

Inten e Use
ExiPrep™ Beef Genomic DNA Kit is developed and supplied for research purposes only.
Certain applications possible with this kit may require special approval by appropriate local
and/or national regulatory authorities in the country of use.
Safety Warning an Precaution
Wear appropriate protection when handling any irritant or harmful reagents. The use of a
laboratory coat, protective gloves and safety goggles are highly recommended. For more
information, please consult the appropriate Material Safety Data Sheet (MSDS).
Warranty an Liability
All BIONEER products undergo extensive Quality Control testing and validation. BIONEER
guarantees quality during the warranty period as specified, when following the appropriate
protocol as supplied with the product. It is the responsibility of the purchaser to determine the
suitability of the product for its particular use. Liability is conditional upon the customer
providing full details of the problem to BIONEER within 30 days.
Quality Management System ISO 9001 Certifie
Every aspect of our quality management system from product development, production to
quality assurance and supplier qualification meets the world-class standards.
Patent
ExiPrep™ and its kits are protected by the patents KR10-201 -0089172.
Tra emark
ExiPrep™ is a trademark of BIONEER Corporation.
Copyright
Copyright 2022 BIONEER Corporation. All Rights Reserved.
Notice
BIONEER corporation reserves the right to make corrections, modifications, improvements
and other changes to its products, services, specifications or product descriptions at any time
without notice.

ExiPrep™ Beef Genomic DNA Kit
Contents
Pro uct Information ……………………………………………………………………………. 1
Components ………………………………………………………………………………….. 1
Storage ……………………………………………………………………………………… 1
Specifications………………………………………………………………………………… 2
Precautions ………………………………………………………………………………….. 2
Intro uction ……………………………………………………………………………………… 3
Product Description …………………………………………………………………………. 3
Principle ………………………………………………………………………………………. 4
Features & Benefits ………………………………………………………………………….
Components of Buffer Cartridges ………………………………………………………….. 6
Experimental Proce ures ……………………………………………………………………… 7
Preparing Sample from Animal Tissues …………………………………………………… 7
Loading the Kit to the Instrument ………………………………………………………….. 8
Troubleshooting ………………………………………………………………………………… 12
Or ering Information …………………………………………………………………………… 13
Relate Pro ucts ……………………………………………………………………………….. 13
Explanation of Symbols ……………………………………………………………………….. 14

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BQ-042-101-01
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Pro uct Information
Components
Components Amount
Buffer Cartridge ① 6 ea
Buffer Cartridge ② 6 ea
Beef Lysis Buffer 2 ml x 1 ea
Proteinase K powder, lyophilized 20 mg x 2 ea
Disposable Filter Tip 3 packs (32 ea/pack)
Reaction Tube (0. ml) 1 pack (96 ea/pack)
Elution Tube 8-tube strips x 12 ea
User Guide 1 ea
* Note: All provided consumables including disposable tips, reaction tubes, and elution tubes are DNase-
and RNase-free.
Storage
The kit will maintain performance for at least two years under standard storage conditions.
The Buffer Cartridges can be stored dry at room temperature (1 -2 °C) for up to 2 years from
the date of delivery, provided they remain sealed.
This product also contains lyophilized enzymes (Proteinase K and RNase A), which are pre-
loaded into Buffer Cartridges (RNase A) and 2 ml screw cap tubes (Proteinase K). They can be
stored at room temperature up to 2 years without any reduction in activity, provided they remain
unopened. Once dissolved, enzymes should be stored at -20°C for up to 6 months.

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BQ-042-101-01
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ExiPrep™ Beef Genomic DNA Kit
Specifications
Sample Type Amount of
Starting Sample
Typical DNA
Yiel DNA Purity
Animal tissue (Bovine muscle) 10-40 mg -10 µg A
260
/A
280
> 1.8
* Note: There may be differences in measured values depending on the type of samples.
Precautions
The Buffer Cartridge ①and ② of this kit are covered with sealing film in order to prevent
cross-contamination, evaporation, or leakage of solutions inside. All of the plastic products and
buffers in this kit are provided under nuclease-free condition, hence, please be careful not to
contaminate any part of the kit with nuclease.

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Intro uction
Pro uct Description
ExiPrep™ Beef Genomic DNA Kit is designed for extraction of highly purified total DNA from
animal tissues. ExiPrep™ Beef Genomic DNA Kit provides total solution for accurate and rapid
total DNA extraction. The kit employs our unique Buffer Cartridge system. The Buffer Cartridges
contain all components for nucleic acid extraction, including: binding buffer, washing buffer,
elution buffer, and magnetic nanobead solution. The Buffer Cartridges are key to extract total
DNA with the aid of ExiPrep™16 (Cat. No. A- 010). ExiPrep™16 is designed for rapid extraction
of nucleic acids delivering up to 16 extracted samples automatically. The process does not
require phenol/chloroform extraction and ethanol precipitation.
DNA extracted through this kit can be used for a variety of applications, including: gene cloning,
PCR, Real-time PCR, genetics, and SNP genotyping.

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BQ-042-101-01
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ExiPrep™ Beef Genomic DNA Kit
Principle
ExiPrep™ Beef Genomic DNA Kit is designed for extraction of genomic DNA. The kit employs
Magnetic Nano Beads coated with silica for nucleic acid binding in the presence of chaotropic
salts. Guanidine hydrochloride as chaotropic agents in binding buffer removes water molecules
around DNA and surface of the silica-coated magnetic nanobeads resulting in genomic DNA,
which is captured by silica-coated magnetic nanobeads. The magnetic nanobeads and DNA
complexes are pulled and fixed on the tube wall using a magnetic force. Any salts and
precipitates are eliminated by washing buffer, and captured genomic DNA is eluted in an elution
buffer or nuclease-free water.

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Features & Benefits
 Comprehensive: High quality and yield of genomic DNA extraction from various samples such
as animal tissues.
 Convenient & Rapid: Uses a pre-filled buffer cartridge system in which enzymes and reagents
for nucleic acid extraction are dispensed.
 Reproducible: Uses fully automatic nucleic acid extraction equipment, and reproducible
results can be obtained.
 Efficient: Contains all required consumables such as Disposable Filter Tips and Elution
Tubes.
 Ready-to-use: Extracted DNA is ready-to-use for various application.

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BQ-042-101-01
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ExiPrep™ Beef Genomic DNA Kit
Components of Buffer Cartri ges
Figure 1. Position of reagents, tubes, an starting material in Cartri ges/ Elution Tube
Rack of ExiPrep™ Beef Genomic DNA Kit (K-3200-CB).

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BQ-042-101-01
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Experimental Proce ures
Preparing Sample from Animal Tissues
This protocol is designed for extraction of genomic DNA from animal tissues (muscle, liver,
kidney, spleen, heart, tail, etc.).
Before beginning, completely dissolve 20 mg of Proteinase K powder in 1 ml of DNase- and
RNase-free water.
1. Grind 10-40 mg of animal tissue sample in liquid nitrogen
†
to a fine powder with a mortar and
pestle and place it into a 1. ml tube.
* Note: Do not allow the sample to thaw.
†
After grinding, liquid nitrogen should be evaporated.
2. Add 2 0 µl of Beef Lysis Buffer
†
and 20 µl of Proteinase K (20 mg/ml) to the sample.
* Note: The sample should be completely immersed in the buffer.
†
Storage of Beef Lysis Buffer at lower temperatures may cause precipitation. If precipitated, heat the
buffer at 60°C to re-dissolve.
3. Incubate at 60°C for at least 2 hours with a shaking water bath.
4. Centrifuge at 13,000 rpm for minutes to remove any precipitates.
. Transfer the cleared lysate to a new 1. ml tube (not provided).
6. Proceed immediately to “Loading the Kit to the Instrument” on page 8.

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ExiPrep™ Beef Genomic DNA Kit
Loa ing the Kit to the Instrument
Steps Proce ure Details
Example 1) For 1 sample
Example 2) For 8 samples
Example 3) For 16 samples
1. Punch holes in the sealing films of Buffer Cartridge ①
and ② using 6-Hole Punch (ExiPrep™ 16 ’s accessory)
according to the number of samples.
* Note: Before punching the hole, agitate the Buffer Cartridge
gently to settle the beads and buffer.
2. Load 200 µl of samples prepared in page 7 into ‘Sample
loading well’ of Cartridge ①.
* Note: Be careful not to contaminate any other wells.
3. Open the door of ExiPrep™ 16 and pull out the
baseplate completely.

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BQ-042-101-01
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4. Place the Reaction Tube Rack including Reaction Tubes
onto the proper position of the baseplate.
. Place the Buffer Cartridge ② onto the proper position of
the baseplate.
* Note: Please check the punched holes of the Buffer
Cartridge ②.
6. Place the Buffer Cartridge ① onto the proper position of
the baseplate.
7. Place the Elution Tube Rack including Elution Tubes onto
the proper position of the baseplate. The Elution Tube
Rack is slotted so it can only be placed in the correct
orientation.
* Note: Make sure the direction of the Elution Tube caps laid
out as on the left when inserting into the Elution Tube Rack.

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BQ-042-101-01
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ExiPrep™ Beef Genomic DNA Kit
8. Place the Disposable Filter Tip Rack onto the proper
position of the baseplate.
* Note: Tips should be placed in the corresponding positions
with the punched holes of the Cartridges.
9. Place the Waste tray between Buffer Cartridge ① and ②.
10. Finally, confirm holes in the cartridges and position of
samples and tips. Push the baseplate completely until
you hear the click sound, then close the door.
11. Turn on the ExiPrep™ 16.
12. In the MENU screen, press ‘Start’ button to select a
proper protocol.
13. The PREP SETUP screen appears as shown in the
left, and a screen to select the protocol number for each
kit appears. Press ‘102’ to confirm that the following
information is displayed on the screen, and then press
the ‘Enter’ button.
Prep Type: Genomic DNA
Sample SRC: Animal tissue
14. Select the desired elution volume from the touch screen.
1 . Press the ‘ok’ button to move to the next step.

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16. Ensure that everything is correctly installed following
the CHECK LIST, then choose “ok”.
17. In the Running Mode screen, ensure that the protocol
name appears on the screen.
18. Press the “RUN” button to initiate DNA extraction.
19. “Work Completion” screen appears when the protocol
is completed. Open the door and collect final DNA from
the Elution Tubes.
20. Remove all components used in the experiment, and
choose 1, 2, or ok.
* Note: If you want to quit and press the ‘ok’ button, the UV
lamp will be turned on automatically.

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BQ-042-101-01
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ExiPrep™ Beef Genomic DNA Kit
Troubleshooting
Problem Comments
Low DNA yiel or
purity
 You may have use too much (or too little) starting material.
DNA yield is dependent on the sample type and amount of starting
sample. Appropriate amount of starting sample should be used for
efficient extraction of genomic DNA.
 The lysis may have been incomplete.
Centrifuge completely to obtain clear lysate.
 Incomplete suspension of the magnetic nanobea s may
ecrease the DNA yiel or purity.
You should agitate the Buffer Cartridge ① before use.
Co-elute magnetic
nanobea s
Sometimes magnetic nanobeads are eluted with your genomic
DNA. Magnetic nanobeads in the eluate will not affect the
performance of the genomic DNA in downstream applications.
Furthermore, magnetic nanobeads cannot bind genomic DNA in
elution buffer, though it may affect readings on a
spectrophotometer. Magnetic nanobeads that are carried over can
be easily separated by centrifugation at 13,000 rpm for 1 minute.

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BQ-042-101-01
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Or ering Information
Description Cat. No
ExiPrep™ Beef Genomic DNA Kit K-3200-CB
Relate Pro ucts
Description Cat. No
ExiPrep™16 A- 010
ExiProgen™ A- 041
Proteinase K Powder KB-0111

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ExiPrep™ Beef Genomic DNA Kit
Explanation of Symbols
Batch
Code
Biological
Risks
Catalog
Number
Caution
Consult
Instructions
For Use
Contains
Sufficient for
<n> tests
Do not
Re-use
Manufacturer
Research
Use
Only
Temperature
Limitation
Use-by
Date

© Copyright 2022 BIONEER Corporation. All rights reserved.