Bioneer ExiPrep 16 Dx User manual

User Manual(Ver 1.0)
ExiPrep™16 Dx
Fully Automated Nucleic Acid Extraction System

Copyright © 2011 by Bioneer Corporation. All rights reserved.
Contents
I. Getting Started ............................................................................................................... 1
II. Safety Warnings and Precautions ..................................................................................... 2
1. User and experimental precautions ............................................................................................................................. 2
2. Precautions regarding the operation environment ............................................................................................... 2
3. Precautions and warnings regarding instrument installation ........................................................................... 3
4. Precautions and warnings regarding instrument operation .............................................................................. 3
5. Precautions and warnings regarding product usage and maintenance ...................................................... 4
6. UV Lamp .................................................................................................................................................................................. 5
III. System Components and Specifications ........................................................................... 6
1. System Components .......................................................................................................................................................... 6
2. Specifications ......................................................................................................................................................................... 7
3. System Views ......................................................................................................................................................................... 7
IV. Installing the ExiPrep™16 Dx ........................................................................................... 8
V. DNA/ RNA Extraction .................................................................................................... 11
1. Contamination Protection Accessory Installation................................................................................................ 11
2. Sample Preparation ......................................................................................................................................................... 12
3. RUN (Not used PC) .......................................................................................................................................................... 14
VI. ExiStation™ Manager Software ...................................................................................... 18
VII. ExiPrepTM16 Dx Setup ................................................................................................... 24
1. Main Menu .......................................................................................................................................................................... 24
2. Registering a New User ................................................................................................................................................. 28
3. Viewing Run History ........................................................................................................................................................ 29
4. Managing the Login Mode .......................................................................................................................................... 30
5. Managing the Automatic UV-Sterilization Mode ................................................................................................ 31
6. Configuring the System ................................................................................................................................................. 32
7. Administrator Management ......................................................................................................................................... 33
VIII. Updating the ExiPrepTM 16 Dx ......................................................................................... 35
IX. DNA/ RNA Extraction Program Number List ..................................................................... 38
X. Troubleshooting ........................................................................................................... 42
XI. Warranty ..................................................................................................................... 46

ExiPrepTM16 Dx
Fully Automated Nucleic Acid Extraction System
A4(210X297)
-1-
I. Getting Started
Thank you for purchasing this Bioneer product.
We will try our best to provide satisfactory results to our customers.
This manual contains practical guidelines and cautions to be taken regarding the instrument.
Please read this manual carefully and thoroughly before using the instrument.
Website
▪Please visit us online at http://www.bioneer.com to obtain more information about ExiPrepTM16
Dx.
General information
▪ExiPrep™16 Dx is a trademark of Bioneer Corporation.
▪The information contained in this manual is under copyright protection. It is unlawful to
reproduce part or all of the contents of this manual without the expressed written consent of
Bioneer Corporation.
▪Bioneer Corporation reserves the right to alter, modify and otherwise make changes to the
instrument and manuals without prior notice.
▪You must be used carefully UV Lamp. Detail can be found in Safety warning and Precautions.
Reversion
Cat. Number A-5050 Rev. 1.0
March/2011

ExiPrepTM16 Dx
Fully Automated Nucleic Acid Extraction System
A4(210X297)
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II. Safety Warnings and Precautions
The warnings and precautions stated below are for the correct and safe operation of the instrument. Please
heed all information for your safety. Bioneer Corporation does not assume responsibility for non-compliance
with the safety warnings and precautions stated below.
1. User and experimental precautions
1) Make sure that the power supply (100-240V, ~50/60Hz) is correctly connected to the power adapter
and that the power adapter is correctly connected to the instrument. Incorrect connection of the
power adapter and power supply can result in instrument damage or failure to turn on.
2) This instrument is intended for nucleic acid extraction. Please use as such.
3) The instrument may stop if the LCD panel is touched while connected to a PC via LAN cable. If
operating the instrument via PC software, please allow for the instrument to finish its programmed
movement before operating the LCD panel.
4) Do not turn the PC off or disconnect the LAN cable connecting the PC to the instrument. Data
communication error can result in instrument malfunction and can affect the results of your
experiment.
5) Please install the instrument on a flat surface.
6) Do not operate the instrument with wet hands as this may result in shock or instrument malfunction.
Please touch the power adapter cord with dry hands.
7) If the instrument is stopped either from operator error including improper accessory insertion or
manually halting the instrument during normal operation, you must re-initialize the instrument before
pulling out the Base Plate. Pulling out the Base Plate without prior initialization can lead to
instrument damage from movement interferences including a raised Heating Block or other
accessories stopped in motion. If Buffer Cartridges are inserted into the Base Plate, please
reinitialize the instrument or pull out the Buffer Cartridges to make sure the Heating Block is not in
the way of normal Base Plate movement.
8) Avoid placing objects in the front and rear of the instrument, as fan blockage may cause lowered
efficiency of the Cooling Block.
9) Be careful not to have any obstructions or foreign materials introduced into the front-lower side
Cooling Fan mesh. Foreign objects can hinder normal Cooling Fan operation and may lead to
overheating of parts or cause a fire.
2. Precautions regarding the operation environment
1) If the power plug is loose, do not use the instrument. Plug overheating may result in shock or fire.
2) Do not operate multiple instruments out of a single wall outlet. The load may cause overheating and
may lead to fire.
3) When plugging or unplugging the power adapter cord from a wall outlet, make sure your hands are

ExiPrepTM16 Dx
Fully Automated Nucleic Acid Extraction System
A4(210X297)
-3-
completely dry. Wet or moist hands may cause electric shock.
4) Avoid placing objects in the front and rear of the instrument.
5) Avoid installing the instrument in a dusty environment. Excessive dust may cause malfunction or
damage to the instrument.
6) Avoid installation near heat sources. This can cause fire.
7) Avoid installation near sources of water or damp locations. This can cause electrical shock, fire or
instrument malfunction.
8) Do not install near sources of flammable or corrosive gas. If there is a gas leak, do not touch the
power plug but open a window to circulate fresh air. Sparks from the power plug can cause fire and
explosions.
9) Do not disassemble or modify the instrument in any way. This can result in fire, electrical shock or
malfunction, and also voids your warranty.
3. Precautions and warnings regarding instrument installation
1) This is a precision instrument. Do not install in a location exposed to direct sunlight.
2) Install the instrument on a flat, solid surface that is flat and does not move.
3) When installing the instrument, make sure at least 15 cm separate the instrument from the nearest
wall.
4) Take caution not to damage the cooling fan mesh (located on the front-bottom) while installing.
5) When connecting the PC, network hub and ExiPrepTM16 Dx, do so with all involved components
powered off. Connecting components while the power is on may cause damage to the instrument.
6) Verify the network cable integrity when connecting between the PC, network hub and ExiPrepTM16
Dx. Unstable network connections may result in invalid experimental data from data communication
errors.
4. Precautions and warnings regarding instrument operation
1) Dust off the power plug and insert the plug so that the connection is firm and does not wiggle.
Incomplete electrical contacts may cause fire.
2) Operate the instrument in an ambient temperature range of 15 ~30 . Excessive exposure to heat℃℃
may affect the instrument and yield inexact results.
3) Operate the instrument within the recommended humidity range (20~80%, no condensation). Humid
conditions may cause corrosion or malfunction.
4) Do not place any objects next to or behind the instrument. The instrument may malfunction.
5) This instrument contains precision machined parts. Do not drop or severely agitate the instrument.
This can break the instrument and compromise the safety of the product.
6) When not using the instrument for a long period of time, turn the instrument off and unplug from the
wall outlet. Overheating and fire may occur.
7) Take caution not to damage the cooling fan mesh located on the front-bottom of the instrument. If