Bioneer ExiPrep K-3200-CB User manual

.
K-3200-CB
ExiPrep
TM
Beef Genomic DNA Kit

ExiPrep
TM
Beef Genomic DNA Kit
ExiPrep
TM
Beef Genomic DNAKit
User’s Guide
Version No.: 3.0 (2019-09-06)
Please read all the information in booklet before using the unit
Bioneer Corporation
8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon
34302, Republic of Korea
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.bioneer.com

ExiPrep
TM
Beef Genomic DNA Kit
Intended Use
ExiPrep
TM Beef Genomic DNA Kit
is developed and supplied for research purposes only. Certain applications
possible with this kit may require special approval by appropriate local and/or national regulatory authorities in
the country of use.
Safety Warning and Precaution
Wear appropriate protection when handling any irritant or harmful reagents. The use of a laboratory coat,
protective gloves and safety goggles are highly recommended. For more information please consult the
appropriate Material Safety Data Sheet (MSDS).
Warranty and Liability
All Bioneer products undergo extensive Quality Control testing and validation. Bioneer guarantees quality during
the warranty period as specified, when following the appropriate protocol as supplied with the product. It is the
responsibility of the purchaser to determine the suitability of the product for its particular use. Liability is
conditional upon the customer providing full details of the problem to Bioneer within 30 days.
Trademark
ExiPrep ™is a trademark of Bioneer Corporation.
Copyright
Copyright 2019. Bioneer Corporation. All Rights Reserved
Notice
Bioneer corporation reserves the right to make corrections, modifications, improvements and other changes
to its products, services, specifications or product descriptions at any time without notice. All information
provided here is subject to change without notice.

ExiPrep
TM
Beef Genomic DNA Kit
Contents
I. Kit components…………………………………………………………………
1
II. Introduction………………………………………………………………………
1
III. Storage…………………………………………………………………………
2
IV. Starting amount and typical yield …………………………………………
2
V. Genomic DNA extraction ……………………………………………………
3
VI. Troubleshooting ………………………………………………………………
6
VII. Explanation of symbols ……………………………………………………
6

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BQ-042-101-01
Revision : 3 (2018-05-24)
ExiPrep
TM
Beef Genomic DNA Kit
I. Kit components
II. Introduction
ExiPrep™ Beef Genomic DNA Kit is suitable to extract genomic DNA from animal tissue using the automatic
nucleic acid extraction instrument, ExiPrep ™ or ExiPrep ™Plus.
ExiPrep ™Beef Genomic DNA Kit
(K-3200-CB)
Buffer Cartridge ①
6 ea
Buffer Cartridge ②
6 ea
Beef Lysis Buffer
1 ea
Proteinase K (20 mg)
2 ea
Disposable Filter Tip
96 ea
Reaction Tube (0.5 ml)
96 ea
Elution Tube (8-strip)
12 ea
User’s Guide
1 ea

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BQ-042-101-01
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ExiPrep
TM
Beef Genomic DNA Kit
III. Storage
ExiPrep ™ Beef Genomic DNA Kits are available in a buffer cartridge form. The buffer cartridge contains washing
buffer, elution buffer and magnetic bead solution optimized for nucleic acid extraction. It is covered and sealed
with aluminum foil film to protect leakage, evaporation and cross contamination. The sealed cartridge can be
stored dry at room temperature (15 - 35℃) for up to 2 years.
ExiPrep ™ Beef Genomic DNA Kits provide lyophilized enzymes (Proteinase K, RNase A) for user’s convenience.
Lyophilized enzymes (Proteinase K, RNase A) are contained in 2ml screw cap tubes and enclosed in the buffer
cartridge. It can be stored at room temperature (15 - 35℃) up to 2 years. Once dissolved, enzymes must be
stored at -20℃.Disposable tips, reaction tubes and elution tubes are also provided which are DNase and RNase
free.
IV. Starting amount and typical yield
Amounts of starting sample volume, elution volume and typical yield are described as below.
Sample type
Starting amount
Elution volume
Typical yield
Animal tissue
(Bovine muscle)
10 –40 mg
50 l
5 –10 g

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BQ-042-101-01
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ExiPrep
TM
Beef Genomic DNA Kit
V. Genomic DNA Extraction
This protocol is designed for extraction of genomic DNA from animal tissues (muscle, liver, kidney, spleen,
heart, tail etc.). This protocol requires shaking water bath and table top centrifuge. Firstly, dissolve 20 mg of
Proteinase K into 1 ml of DNase, RNase free water. Beef lysis buffer may form precipitates during storage.
Please warm the buffer at 60℃ until precipitates are completely dissolved.
Tissue samples can be disrupted first by pestle & mortar or any other tissue disruptor.
1. Add liquid nitrogen into the mortar to cool the mortar and pestle.
2. Cut 10 –40 mg of animal tissue and transfer into the mortar.
3. Grind to a fine powder.
4. Transfer the powdered tissue into 1.5 ml test tube (not provided).
5. Add 20 l of Proteinase K and 250 l of Beef lysis buffer into the tube.
6. Incubate the tube at 60℃ for at least 2 hours in shaking.
※We recommend O/N incubation for complete lysis.
7. Centrifuge the tube at 13,000 rpm for 5 min to remove any remaining tissue
that is not lysed.
8. During step 7, place the reaction tube rack (B), elution tube rack (C),
disposable tip rack (D) and buffer cartridge ① (E) onto the setup tray (A).
9. Load the reaction tubes (F), elution tubes (G) and disposable filter tips (H)
into each of the racks.
10. Ensure that all tips and tubes are aligned and positioned in the right
direction.
11. Load 200 l of sample into the sample loading wells.
Be careful not to contaminate other wells.

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BQ-042-101-01
Revision : 3 (2018-05-24)
ExiPrep
TM
Beef Genomic DNA Kit
12. Place the reaction tube rack in the base plate.
13. Punch holes in the buffer cartridge according to the number of samples
②.
14. Place the buffer cartridge ① in the base plate.
15. Place the elution tube rack in the base plate.
16. Place the disposable filter tip rack in the base plate.
17. Place the waste tray in the base plate between the two buffer cartridges,
① and ②.
18. Push the base plate back into the instrument and close the door.

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BQ-042-101-01
Revision : 3 (2018-05-24)
ExiPrep
TM
Beef Genomic DNA Kit
19. Turn on the ExiPrep ™ or ExiPrep ™Plus.
20. Press the ‘Start’ button to access the PREP SETUP menu.
21. Enter a protocol number 102.
22. Press the ‘Enter’ button.
23. Select the elution volume on the touch screen.
24. Press the ‘ok’ button.
25. Follow the instructions according to ‘CHECK LIST’ and confirm.
26. Verify the protocol name on the screen.
27. Press the ‘Run’ button to start.
28. After completion, take the elution tubes from the base plate first.
29. Remove all remaining parts from the base plate and close the door.

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BQ-042-101-01
Revision : 3 (2018-05-24)
ExiPrep
TM
Beef Genomic DNA Kit
VI. Troubleshooting
1. Low yield of Genomic DNA
1) Yield varies by sample type and amount. Check if you added sufficient amount of sample? Sometimes
too much sample may decrease yield.
2) Did you completely lysis sample? Insufficient lysis decreases yield and purity.
3) Is there precipitated salt in the lysis buffer, or resuspension buffer? Keep the bottles at 60℃ to re-
dissolve.
4) Did you shake your buffer cartridge ① before use? Incomplete suspension of the magnetic beads may
decrease yield and purity.
2. Co-eluted magnetic particle
Sometimes magnetic particles are co-eluted with your extracted DNA. These magnetic particles, however,
won’t affect yield or purity, as they don’t bind viral DNA and RNA in elution buffer.
Co-eluted magnetic particles can easily separate by simple centrifugation.
VII. Explanation of symbols
Catalog
Number
Contains sufficient for (n)
tests
USE BY
Batch code
Caution, consult
accompanying documents
Temperature Limitation
Manufacturer
Caution, Potential
Biohazard
DO NOT
REUSE
Consult Instruction For
Use

ExiPrep
TM
Beef Genomic DNA Kit
8-11Munpyeongseo-ro,Daedeok-gu,Daejeon,34302,RepublicofKorea
[phone](Korea:1588-9788)
[phone]
[e-mail]
www.bioneer.com
155FilbertSt.Suite216Oakland,CA94607,USA
[phone](Toll-free)
[phone]
[e-mail]
us.bioneer.com
KoreaBioParkBLDG#B-702,700Daewangpangyo-ro,Bundang-gu,Seongnam-si
Gyeonggi-do,13488,RepublicofKorea
[phone]
[phone]
[e-mail]
www.bioneer.co.kr