Bioneer AllInOneCycler User manual

I

AllInOneCycler
TM
/ Thermal Block
www.bioneer.com
BQ-042-101-01
Revision : 0(2012-12-07)
I
Bioneer Corporation
8-11, Munpyeongseo-ro, Daedeok-gu,
Daejeon, 34302, Republic of Korea
Tel: [phone]
Fax: [phone]
Email: [e-mail]
www.bioneer.com

AllInOneCycler
TM
/ Thermal Block
www.bioneer.com
BQ-042-101-01
Revision : 0(2012-12-07)
AllInOneCycler ™

AllInOneCycler
TM
/ Thermal Block
www.bioneer.com
BQ-042-101-01
Revision : 0(2012-12-07)
Contents
I. Getting Started ............................................................................................................... 1
II. Symbols on the AllInOneCycler ™....................................................................................... 3
III. Safety Warnings and Precautions....................................................................................... 5
1. User and experimental precautions .........................................................................5
2. Precautions regarding the electrical environment ........................................................5
3. Precautions regarding the operation environment........................................................6
4. Precautions and warnings regarding instrument installation ...........................................7
5. Precautions and warnings regarding instrument operation .............................................7
6. Precautions and warnings regarding product usage and maintenance ..............................7
7. Precautions regarding moving and lifting instrument ....................................................8
8. Warnings regarding biological and chemical hazard safety ............................................8
IV. System Components and Specifications ........................................................................... 10
1. System Components ........................................................................................ 10
2. Specifications ................................................................................................. 12
3. System Views ................................................................................................. 13
V. Installing the AllInOneCycler
TM ......................................................................................... 14
1. Site Requirements ............................................................................................ 14
2. Required Materials............................................................................................ 14
3. Unpacking the AllInOneCycler
TM ........................................................................... 14
4. Setting Up the AllInOneCycler
TM ........................................................................... 15
5. Power ON ...................................................................................................... 16
VI. Operating the AllInOneCycler
TM........................................................................................ 17
1. Loading Samples into the Instrument..................................................................... 17
2. Home menu ................................................................................................... 18
3. Editing Protocols ............................................................................................. 19
4. Running the PCR protocols ................................................................................. 36
5. Removing the tubes or plate from the AllInOneCycler
TM .............................................. 38
6. Setting menu .................................................................................................. 39
7. About menu ................................................................................................... 47
8. Network Operating AllInOneCycler
TM ...................................................................... 48
VII. Troubleshooting ............................................................................................................ 55
VIII. User Maintenance ......................................................................................................... 58
1. Cleaning the main body ..................................................................................... 58
2. Cleaning the Hot-top Cover ................................................................................ 59
3. Replacing Fuses .............................................................................................. 59
4. Returning an AllInOneCycler
TM for Service ............................................................... 61
IX. Warranty...................................................................................................................... 62

AllInOneCycler
TM
/ Thermal Block
1
www.bioneer.com
BQ-042-101-01
Revision : 0(2012-12-07)
I. Getting Started
Thank you for purchasing this Bioneer product.
We will try our best to provide satisfactory results to our customers.
This manual contains practical guidelines and cautions to be taken regarding the instrument.
Please read this manual carefully and thoroughly before using the instrument.
Website
Please visit us online at http://www.bioneer.com to obtain more information about
AllInOneCycler
TM.
General information
 AllInOneCycler
TM is a trademark of Bioneer Corporation.
The information contained in this manual is under copyright protection. It is unlawful to
reproduce part or all of the contents of this manual without the expressed written
consent of Bioneer Corporation.
Bioneer Corporation reserves the right to alter, modify and otherwise make changes to
the instrument and manuals without prior notice.
You must use the supplied accessories and tubes.
 AllInOneCycler
TM can be used multi system use to PC software.
Safety Labels Description on the AllInOneCycler ™
English
Do not touch the surface. It is extremely hot.
Francais
Surface chaude
Faites attention à la surface chaude.
English
Do not operate the bath on at low temperature for a long time.
This will produce condensation, which may cause malfunction.
Francais
Ne pas utiliser le bain à basses températures pendant plusieurs heures.
Risque de condensation pouvant entrainer des fonctionnements défectueux de
l’équipement.
English
Be aware of the door moving.
This may cause injury.
Francais
Manipulation de la porte
Risque de blessures corporelles (pincement/écrasement) lors de
l’ouverture/fermeture de la porte.

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TM
/ Thermal Block
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BQ-042-101-01
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About the AllInOneCycler ™
AllInOneCycler
TM Thermal Block is a multifunctional, peltier-based thermal block, equipped with a
temperature control system. AllInOneCycler
TM Thermal Block has various functions including
temperature increment, time increment and ramp rate control. This system is compact in size and
light weight for easy storage and moving. It has user friendly interface with TFT-LCD touch screen
to allow users to program easily and conveniently. AllInOneCycler
TM Thermal Block also can be
controlled with optional PC software which is supplied separately. AllInOneCycler
TM is equipped
with communication functions between PC and instrument using TCP/IP protocol, providing full
operations from a remote location. Users can create PCR protocols on computer that can be
loaded onto instrument at a later time.
Intended purpose
Amplification of specific DNA that is included in samples.

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II. Symbols on the AllInOneCycler ™
Symbols on the AllInOneCycler ™user manual
The following table describes the symbols that may be displayed on AllInOneCycler
TM user manual.
Conformité Européenne Mark
In vitro diagnostic medical device
Temperature limitation
Manufacturer
Authorized representative in the European Community
Catalog number
Safety Symbols on the AllInOneCycler ™
The following table describes the safety symbols that may be displayed on AllInOneCycler
TM.
Each symbol may appear by itself or with text that explains the relevant hazard.
Do not touch the surface.
It is extremely hot.
Do not operate the bath on at low temperature for a long time.
This will produce condensation, which may cause malfunction.
Be aware of the door moving.
This may cause injury.

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TM
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Electrical Symbols on the AllInOneCycler ™
The following table describes the electrical symbols that may be displayed on the AllInOneCycler
TM.
Indicates the On position of the main power switch.
Indicates the Off position of the main power switch.
Indicates a protective grounding terminal in the instruments that must be
connected to earth ground.
Indicates a terminal that can receive or supply alternating current or voltage.
Indicates a terminal that can receive or supply alternating or direct current or
voltage.
Environmental Symbols on the AllInOneCycler
TM
The following symbol (WEEE) applies to the AllInOneCycler
TM placed on the European market.
Do not dispose of AllInOneCycler ™as unsorted municipal waste.
Follow local municipal waste ordinances for proper disposal provisions to
reduce the environmental impact of waste electrical and electronic
Instrument.
European Union customers:
Call your local Europe office for Bioneer instruments pick-up and recycling.

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III. Safety Warnings and Precautions
The warnings and precautions stated below are for the correct and safe operation of the
instrument. Please heed all information for your safety. Bioneer Corporation does not assume
responsibility for non-compliance with the safety warnings and precautions stated below.
Warning: Hazards or dangerous actions that may result in severe injury.
Caution: Hazards or dangerous actions that may result in minor injury or damage.
Warning: Hazards or dangerous actions that may result in burn.
Danger: Hazards or dangerous actions that may result in electric shock.
1. User and experimental precautions
(1) Make sure that the power supply (100-240VAC, 50/60Hz) is correctly connected to the
power cord and that the power cord is correctly connected to the instrument. Incorrect
connection of the power cord can result in instrument damage or failure to turn on.
(2) This instrument is intended for nucleic acid amplification. Please use as such.
(3) The instrument may stop if the LCD panel is touched while connected to a PC via LAN cable.
If operating the instrument via PC software, please allow for the instrument to finish its
programmed movement before operating the LCD panel.
(4) Do not turn the PC off or disconnect the LAN cable connecting the PC to the instrument
while instrument is running. Data communication error can result in instrument malfunction
and can affect the results of your experiment.
(5) Please install the instrument on a flat surface.
(6) Do not operate the instrument with wet hands as this may result in shock or instrument
malfunction. Please touch the power cord with dry hands.
(7) If you make a mistake such as using an improper accessory, or force the instrument to stop
during a normal operation, the instrument may stop working.
(8) Avoid placing objects in the front and rear of the instrument, as fan blockage may cause
lowered efficiency of the Cooling Block.
(9) Be careful not to have any obstructions or foreign materials introduced into the front, rear
and side Cooling Fan mesh. Foreign objects can hinder normal Cooling Fan operation and
may lead to overheating of parts or cause a fire.
2. Precautions regarding the electrical environment
(1) If the power cord is loose, do not use the instrument. Power cord overheating may result in

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TM
/ Thermal Block
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shock or fire.
(2) Do not operate multiple instruments out of a single wall outlet. The load may cause
overheating and may lead to fire.
(3) When plugging or unplugging the power cord from a wall outlet, make sure your hands are
completely dry. Wet or moist hands may cause electric shock.
(4) A convenient and safe power cord should be available. The power cord provided by our
company should be used.
(5) Socket outlets should be at least 1.5 m from a sink or wash basin.
(6) Power cord cable should not be repaired with insulating tape. Water can still get inside.
(7) If you use other power cord, it should be adequate for the electrical capacity for the
AllInOneCycler
TM (250V, 16A, 0.75 mm2, VDE).
(8) Plugs should match the socket outlets.
(9) Unplug the power cord from the AllInOneCycler
TM when not in operation for a long period of
time to prevent the possibility of fire by overheating.
(10) AllInOneCycler
TM is equipped with a 3-conductor AC power cord that, when connected to an
appropriate AC power outlet, grounds (earths) the instrument. To preserve this protection
feature, do not operate the instrument from an AC power outlet that has no ground (earth)
connection.
(11)Improper fuses or high-voltage supply can damage the instrument wiring system and cause a
fire. Before turning on the instrument, verify that the fuses are properly installed and that the
instrument voltage matches the power supply in your laboratory.
(12)For continued protection against the risk of fire, replace fuses only with fuses (250V,F10AL,
2ea) of the type and rating specified for the instrument.
3. Precautions regarding the operation environment
(1) Avoid placing objects from every which direction the instrument.
(2) Avoid installing the instrument in a dusty environment. Excessive dust may cause
malfunction or damage to the instrument.
(3) Avoid installation near heat sources. This can cause fire.
(4) Avoid installation near sources of water or damp locations. This can cause electrical shock,
fire or instrument malfunction.
(5) Do not install near sources of flammable or corrosive gas. If there is a gas leak, do not
touch the power plug but open a window to circulate fresh air. Sparks from the power plug
can cause fire and explosions.
(6) Do not disassemble or modify the instrument in any way. This can result in fire, electrical
shock or malfunction, and also voids your warranty.

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4. Precautions and warnings regarding instrument installation
(1) This is a precision instrument. Do not install in a location exposed to direct sunlight.
(2) Install the instrument on a flat, solid surface that is flat and does not move.
(3) When installing the instrument, make sure at least 15 cm separate the instrument from the
nearest wall.
(4) When connecting the PC, network hub and AllInOneCycler
TM, do so with all involved
components powered off. Connecting components while the power is on may cause
damage to the instrument.
(5) Verify the network cable integrity when connecting between the PC, network hub and
AllInOneCycler
TM. Unstable network connections may result in invalid experimental data from
data communication errors.
5. Precautions and warnings regarding instrument operation
(1) Dust off the power plug and insert the plug so that the connection is firm and does not
wiggle. Incomplete electrical contacts may cause fire.
(2) Operate the instrument in an ambient temperature range of 15℃~30℃(59~86℉). Excessive
exposure to heat may affect the instrument and yield inexact results.
(3) Operate the instrument within the recommended humidity range (20~80%, no condensation).
Humid conditions may cause corrosion or malfunction.
(4) Do not place any objects next to or behind the instrument. The instrument may malfunction.
(5) This instrument contains precision machined parts. Do not drop or severely agitate the
instrument. This can break the instrument and compromise the safety of the product.
(6) When not using the instrument for a long period of time, turn the instrument off and unplug
from the wall outlet. Overheating and fire may occur.
(7) Set your PC's Power Options for 'Turn off the display', 'Turn off the hard disk', 'Standby
mode' and 'Sleep mode' to 'Never'. Because the instrument continuously transmits and
receives data from the PC, hard disk power-down may cause malfunctions.
(8) Do NOT open the door during instrument’s operation as this may result in the instrument to
malfunction, causing bodily harm, including burns to hands or body.
(9) Moving parts can crush and cut. Keep hands clear of moving parts while operating the
instrument.
6. Precautions and warnings regarding product usage and maintenance

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(1) This product must only be used for nucleic acid amplification. Do not use the instrument for
any use other than explicitly stated in the User Guide.
(2) You must use the supplied accessories and tubes.
(3) Do not modify or delete instrument-related information installed within the instrument.
(4) Operate the LCD touch-screen using a non-sharp object. Nails and other sharp objects may
damage the product.
(5) Do not use powerful detergents or solvents to clean the outside of the instrument as this
may cause discoloration. If such chemicals are spilled on the instrument, immediately clean
with a soft cloth.
(6) Do not keep the instrument in an environment with high humidity. Damage from storage in
these conditions is classified as water damage and is not covered by warranty. Also,
damage arising from this type of exposure may be irreparable.
(7) Disassembly and/or modification of the instrument voids the warranty and the instrument
may be refused service.
(8) Do not unplug the power cord from the instrument while the instrument is in use. This may
cause the instrument to malfunction.
(9) If a burning smell is detected or the instrument seems to be excessively hot during operation,
immediately stop using the instrument and call your service representative.
(10)Do not drop or impact the instrument. This is a direct cause of instrument damage and may
void the warranty.
7. Precautions regarding moving and lifting instrument
(1) The instrument is to be moved and positioned only by the personnel or vendor specified in the
applicable site preparation guide. If you decide to lift or move the instrument after it has been
installed, do not attempt to lift or move the instrument without the assistance of others, the
use of appropriate moving Instrument, and proper lifting techniques.
(2) Improper lifting can cause painful and permanent back injury. Depending on the weight,
moving or lifting an instrument may require two or more persons.
8. Warnings regarding biological and chemical hazard safety
(1) Biological samples such as tissues, body fluids, and blood of humans and other animals
have the potential to transmit infectious diseases. Follow all applicable local, state/provincial,
and/or national regulations. Wear appropriate protective eyewear, clothing, and gloves.
(2) Before handling any chemicals, refer to the Material Safety Data Sheet (MSDS) provided by
the manufacturer, and observe all relevant precautions.

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(3) Always determine what chemicals have been used in the instrument before changing reagents
or instrument components. Wear appropriate eyewear, protective clothing, and gloves when
working on the instrument.
(4) Read and understand the Material Safety Data Sheet (MSDS) provided by the chemical
manufacturer before you store, handle, or work with any chemicals or hazardous materials.
(5) Minimize contact with chemicals. Wear appropriate personal protective Instrument when
handling chemicals (for example, safety glasses, gloves, or protective clothing).

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IV. System Components and Specifications
1. System Components
Figure 1. System components of the AllInOneCycler
TM

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TM
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Table 1. System components of the AllInOneCycler
TM
Part Name
Cat. No.
Qty.
AllInOneCycler
TM PCR system
(Basic) AllInOneCycler
TM 96 well PCR system*
A-2041-1N
1 ea
(Optional) AllInOneCycler
TM 384 well PCR system
A-2041-2N
(Optional) AllInOneCycler
TM Slide PCR system
A-2041-3N
(Optional) AllInOneCycler
TM Fast 96 well PCR system
A-2041-1F
(Optional) AllInOneCycler
TM
Fast 384 well PCR system
A-2041-2F
(Optional) AllInOneCycler
TM
Fast Slide PCR system
A-2041-3F
AllInOneCycler
TM thermal block
(Basic) AllInOneCycler
TM 96 well thermal block only*
A-2041-1-1
1 ea
(Optional) AllInOneCycler
TM 384 well thermal block only
A-2041-2-1
(Optional) AllInOneCycler
TM Slide thermal block only
A-2041-3-1
(Optional) AllInOneCycler
TM Fast 96 well thermal block only
A-2041-1-2
(Optional) AllInOneCycler
TM Fast 384 well thermal block only
A-2041-2-2
(Optional) AllInOneCycler
TM Fast Slide thermal block only
A-2041-3-2
User’s Guide
1 ea
Power Cord
1 ea
(Optional) AllInOneCycler
TM PC control software
A-2041-9
* AllInOneCycler
TM 96 well PCR system (A-2041-1N) is basic model and includes
AllInOneCycler
TM 96 well thermal block (A-
2041-1-1).

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TM
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2. Specifications
Physical specifications
Dimension
246 mm(W) x 232 mm(H) x 369 mm(D)
9.68 in(W) x 9.14 in(H) x 14.53 in(D)
Weight
8.4 kg (18.52 lbs)
Display
7-inch Touch LCD screen
Power consumption
100-240 V~, Max 8.5 A, 50/60 Hz
630 VA
Port*
2 USB ports for storage (front and lower rear)
1 USB port for engineering (upper rear)
Fuses
Two fuses (250 V, F10AL, 5×20m)
Operating temperature
15 °C ~ 30 °C (59 ℉~ 86 ℉)
Operating humidity
20 % ~ 80 %, no condensation
Interchangeable block format†
96-well (0.2 ml), 384-well (0.02 ml) and
Slide PCR type
PCR volume range
5 μl ~ 80 μl
Operating specifications
Method of heating / cooling
Peltier
Temperature range
4.0 °C ~ 99.9 °C (39.2 ℉~ 211.82 ℉)
Maximum ramp rate‡
Heating : 6.5 °C/sec (11.7 ℉/sec)
Cooling : 4.5 °C/sec (8.1 ℉/sec)
Temperature accuracy
± 0.3 °C (±0.54 ℉)
Temperature uniformity
≤0.3 °C (≤0.54℉)
Lid temperature
90 °C ~110 °C (194 ℉~ 230 ℉)
Temperature increment range
0.1 °C ~ 2.0 °C (0.18 ℉~ 3.6 ℉)
Time increment range
1 sec ~ 59 sec
Ramp rate control
0.1 °C/sec (0.18 ℉/sec)
* Upper USB port on the back of the instrument is only for engineer, and the user can not use for saving data files.
†Basic supplied block is AllInOneCycler
TM 96 well thermal block (Cat. No. A-2041-1-1), and other formats of block are
optional. Bioneer provides the interchangeable thermal blocks made of 2 different metals (normal and novel alloy) and
classified to 3 sample-well types (96-well, 384-well and slide PCR).
‡The max ramp rate is using AllInOneCycler
TM 96 well PCR system (Cat. No. A-2041-1N). When using
AllInOneCycler
TM
Fast 96 well PCR system (Cat. No. A-2041-1F), the max block ramp rates are 9.5 °C/sec for heating and 7.7 °C/sec
for cooling.

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3. System Views
Figure 2. Front view of the AllInOneCycler
TM
Figure 3. Back view of the AllInOneCycler
TM

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V. Installing the AllInOneCycler
TM
1. Site Requirements
The AllInOneCycler
TM Thermal Block is for indoor use. Ensure that the installation site.
-Meets the spatial and weight requirements
-Meets environmental requirements
-Is within 1.5 m (4.92 ft) of a 700VA power receptacle
-Is away from water
2. Required Materials
Scissors, pocket knife, or box cutter
3. Unpacking the AllInOneCycler
TM
Save the packing materials and box in case you need to ship the instrument to Bioneer for service.
(1) To unpack the AllInOneCycler
TM:
Figure 4. Package of the AllInOneCycler
TM
1) Cut the straps securing the instrument box.
2) Cut the tape securing the top flaps of the instrument crate, then open the flaps.

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3) Remove the AllInOneCycler
TM accessories from the instrument and set them aside.
4) Lift and remove the cover from the instrument crate.
5) Remove the packing material from the AllInOneCycler
TM, and then inspect the instrument
for shipping damage.
If the AllInOneCycler
TM is damaged, note the location and appearance of the damage, and then
contact Bioneer Technical Support or your service representative.
(2) Move the AllInOneCycler
TM to the desired installation site. Follow these guidelines for lifting
and moving:
Make sure that you have a secure, comfortable grip.
Keep your spine in a neutral position.
Bend at the knees and lift with your legs.
Do not lift and twist your torso at the same time.
(3) Open the box containing the AllInOneCycler
TM accessories, then verify that it contains:
 AllInOneCycler
TM Thermal Block User Guide
Power Cord, Power 10A/125V North America or Power 10A/220V Europe
4. Setting Up the AllInOneCycler
TM
(1) Remove the packing material:
1) Open the AllInOneCycler
TM hot-top cover.
2) Using a can of compressed air, deliver a blast of air into each well of the bath block to
remove any particles that may have collected during transportation.
3) Close the hot-top cover.
(2) Connect the power cord to the AllInOneCycler
TM.
(3) (Optional) Install any of the recommended protective devices.
(4) Connect the power cord to the 700VA power source receptacle.

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5. Power ON
(1) Confirm that the hot-top cover of the AllInOneCycler
TM is closed.
(2) Press the power button on the back left side of the AllInOneCycler
TM.
Figure 5. Power button of the AllInOneCycler
TM
(3) When the initialization of the device is completed, ‘Home’ menu will be displayed on screen.
Figure 6. Home screen of the AllInOneCycler
TM