VWR PerfectBlue Twin S User manual

VWR® PerfectBlue™Vertical Dual Gel
Systems Twin S, M, L & ExW S
Instruction Manual
European Catalogue Numbers:
Twin S
700-0931
Twin S w/o gel caster
700-0937
Twin M
700-0955
Twin M w/o gel caster
700-0961
Twin L
700-1080
Twin L w/o gel caster
700-1205
Twin ExW
700-0980
Twin ExW w/o gel caster
700-0986
Version: 1
Issued: 10,04,2018

1 VWR PerfectBlue Twin ver 1 10,5,2018
Table of Contents
Legal Address of Manufacturer 2
Country of Origin 2
Warranty 2
Safety Precautions 2
Intended Use 2
Packaging List 3
System Overview 4
Technical Properties 4
Short Instruction 5
Systems Overview 5
Leak Proof Sealing of Glass Plates 5
Assembling the System and Electrophoresis 6
General Instructions 7
Leak Proof Sealing of Glass Plates 7
Assembling the System and Electrophoresis 8
Cleaning 9
Required Materials and Recipes 10
Required Gel Volumes 10
Required Buffer Volumes 10
Tris-Glycine SDS-PAGE 10
Tris-Tricine SDS-PAGE 11
Troubleshooting 12
Technical Support and Ordering Information 14
PerfectBlue™ Dual Gel System Twin S 14
PerfectBlue™ Dual Gel System Twin M 15
PerfectBlue™ Dual Gel System Twin L 15
PerfectBlue™ Dual Gel System Twin ExW S 16
Power Supplies 17

2 VWR PerfectBlue Twin ver 1 10,5,2018
Legal Address of Manufacturer
VWR International bvba
Researchpark Haasrode 2020
Geldenaaksebaan 464
B-3001 Leuven
[phone]
http://be.vwr.com
Country of Origin
United States
Warranty
VWR warrants that this product will be free from defects in material and workmanship for a period of three
(3) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or
refund the purchase price of this product to the customer, provided it is returned during the warranty period.
This warranty does not apply if the product has been damaged by accident, abuse, misuse, or
misapplication, or from ordinary wear and tear. If the required maintenance and inspection services are not
performed according to the manuals and any local regulations, such warranty turns invalid, except to the
extent, the defect of the product is not due to such non-performance.
Items being returned must be insured by the customer against possible damage or loss. This warranty shall
be limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE
IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF
MERCHANTABILITY.
Safety Precautions
To avoid electrical shock:
Please, read this Instruction Manual carefully before using the gel system.
Only use a CE marked DC power supply.
Always disconnect the system from the power supply before removing the safety lid.
Always disconnect the gel system from the power supply when it is not in use or before moving it.
Running conditions for this unit should not exceed the maximum operating voltage or current.
The flow rate for cooling should not exceed 2 liter per minute if the chamber is connected to a water tab. If
using a water circulator the flow should not exceed 12 liter per minute.
Intended Use
Use this product only for its intended purpose as described in this manual. Do not use this product if the
power leads are damaged or if any of its surfaces are cracked.
For research use only, Not intended for use in diagnostic or therapeutic applications.

3 VWR PerfectBlue Twin ver 1 10,5,2018
Packaging List
The following items are included in shipment for the models Twin S, Twin M, Twin L and Twin ExW S:
Twin S (700-0937)
Twin M (700-0961)
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
2 notched glass plates
2 notched alumina ceramic plates
4 clamp assemblies
2 combs, 0.8 mm thick, 10 teeth
4 side spacers, 0.8 mm thick
2 bottom spacers, 0.8 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
4 notched glass plates
4 clamp assemblies
2 combs, 1.5 mm thick, 10 teeth
2 combs, 1.5 mm thick, 15 teeth
8 side spacers, 1.5 mm thick
4 bottom spacers, 1.5 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Twin S (700-0931)
Twin M (700-0955)
as the 700-0937 but including casting base
as the 700-0961 but including casting base
Twin ExW S (700-0986)
Twin L (700-1205)
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
4 notched glass plates
4 clamp assemblies
2 combs, 0.8 mm thick, 15 teeth
2 combs, 0.8 mm thick, 20 teeth
8 side spacers, 0.8 mm thick
4 bottom spacers, 0.8 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
4 notched glass plates
4 clamp assemblies
2 combs, 1.5 mm thick, 15 teeth
2 combs, 1.5 mm thick, 20 teeth
8 side spacers, 1.5 mm thick
4 bottom spacers, 1.5 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Twin ExW S (700-0980)
Twin L (700-1080)
as the 700-0986 but including casting base
as the 700-1205 but including casting base

4 VWR PerfectBlue Twin ver 1 10,5,2018
System Overview
PerfectBlue™ vertical dual gel systems Twin S, M, L and ExW S are characterized by sophisticated design
and durable construction. With their unique arrangement of 3 cathodes and an extended anode surrounding
the complete base of the electrophoresis chamber, electrophoretic separations of exceptional high quality
can be achieved. All of the systems are equipped with an alumina ceramic cooling system for water
circulation matching effective cooling and high migration rates without negatively affecting separation
results. Using the casting system developed for the PerfectBlue™ vertical dual gel systems up to 2 gels can
get poured at once, conveniently and without leakage. Designed for convenience, the glass plates do not
have to be re-assembled after polymerization and electrophoresis can performed straight away. This time
saving step further eliminates potential sources of error. PerfectBlue™ vertical dual gel systems are
available in 4 different formats, each one including a wide range of accessories for increased versatility. The
systems are constructed using solid parts, precisely machined from 0.6 mm thick acrylic by CNC milling. The
use of specialized adhesives neutralizes mechanical forces and thermal expansions making the system
durable and reliable. We are therefore confident of our guarantee, a full 3 year warranty on the whole range
of PerfectBlue™ electrophoresis and blotting equipment.
Technical Properties
PerfectBlue™
Cat. No.
Gel size
(W x L)
required
buffer volume
Maximal
Voltage
max. current
per gel
Typical
running time
Twin S
700-0931/
700-0937 10 x 10 cm 250 ml up to 500 V 15 - 35 mA 30 - 90 min
Twin M
700-0955/
700-0961 16 x 14 cm 650 ml up to 500 V 15 - 50 mA 60 - 120 min
Twin L
700-1080/
700-1205 20 x 20 cm 1250 ml up to 500 V 15 - 75 mA 60 - 180 min
Twin ExW S
700-0980/
700-0986 20 x 10 cm 650 ml up to 500 V 30 - 45 mA 30 - 90 min
Safety lid with power cords
Upper buffer chamber (UBC)
with side clamps and electrodes
Lower buffer chamber (LBC)
Casting base