VWR PerfectBlue Twin S User manual

VWR® PerfectBlue™Vertical Dual Gel
Systems Twin S, M, L & ExW S
Instruction Manual
European Catalogue Numbers:
Twin S
700-0931
Twin S w/o gel caster
700-0937
Twin M
700-0955
Twin M w/o gel caster
700-0961
Twin L
700-1080
Twin L w/o gel caster
700-1205
Twin ExW
700-0980
Twin ExW w/o gel caster
700-0986
Version: 1
Issued: 10,04,2018

1 VWR PerfectBlue Twin ver 1 10,5,2018
Table of Contents
Legal Address of Manufacturer 2
Country of Origin 2
Warranty 2
Safety Precautions 2
Intended Use 2
Packaging List 3
System Overview 4
Technical Properties 4
Short Instruction 5
Systems Overview 5
Leak Proof Sealing of Glass Plates 5
Assembling the System and Electrophoresis 6
General Instructions 7
Leak Proof Sealing of Glass Plates 7
Assembling the System and Electrophoresis 8
Cleaning 9
Required Materials and Recipes 10
Required Gel Volumes 10
Required Buffer Volumes 10
Tris-Glycine SDS-PAGE 10
Tris-Tricine SDS-PAGE 11
Troubleshooting 12
Technical Support and Ordering Information 14
PerfectBlue™ Dual Gel System Twin S 14
PerfectBlue™ Dual Gel System Twin M 15
PerfectBlue™ Dual Gel System Twin L 15
PerfectBlue™ Dual Gel System Twin ExW S 16
Power Supplies 17

2 VWR PerfectBlue Twin ver 1 10,5,2018
Legal Address of Manufacturer
VWR International bvba
Researchpark Haasrode 2020
Geldenaaksebaan 464
B-3001 Leuven
[phone]
http://be.vwr.com
Country of Origin
United States
Warranty
VWR warrants that this product will be free from defects in material and workmanship for a period of three
(3) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or
refund the purchase price of this product to the customer, provided it is returned during the warranty period.
This warranty does not apply if the product has been damaged by accident, abuse, misuse, or
misapplication, or from ordinary wear and tear. If the required maintenance and inspection services are not
performed according to the manuals and any local regulations, such warranty turns invalid, except to the
extent, the defect of the product is not due to such non-performance.
Items being returned must be insured by the customer against possible damage or loss. This warranty shall
be limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE
IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF
MERCHANTABILITY.
Safety Precautions
To avoid electrical shock:
Please, read this Instruction Manual carefully before using the gel system.
Only use a CE marked DC power supply.
Always disconnect the system from the power supply before removing the safety lid.
Always disconnect the gel system from the power supply when it is not in use or before moving it.
Running conditions for this unit should not exceed the maximum operating voltage or current.
The flow rate for cooling should not exceed 2 liter per minute if the chamber is connected to a water tab. If
using a water circulator the flow should not exceed 12 liter per minute.
Intended Use
Use this product only for its intended purpose as described in this manual. Do not use this product if the
power leads are damaged or if any of its surfaces are cracked.
For research use only, Not intended for use in diagnostic or therapeutic applications.

3 VWR PerfectBlue Twin ver 1 10,5,2018
Packaging List
The following items are included in shipment for the models Twin S, Twin M, Twin L and Twin ExW S:
Twin S (700-0937)
Twin M (700-0961)
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
2 notched glass plates
2 notched alumina ceramic plates
4 clamp assemblies
2 combs, 0.8 mm thick, 10 teeth
4 side spacers, 0.8 mm thick
2 bottom spacers, 0.8 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
4 notched glass plates
4 clamp assemblies
2 combs, 1.5 mm thick, 10 teeth
2 combs, 1.5 mm thick, 15 teeth
8 side spacers, 1.5 mm thick
4 bottom spacers, 1.5 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Twin S (700-0931)
Twin M (700-0955)
as the 700-0937 but including casting base
as the 700-0961 but including casting base
Twin ExW S (700-0986)
Twin L (700-1205)
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
4 notched glass plates
4 clamp assemblies
2 combs, 0.8 mm thick, 15 teeth
2 combs, 0.8 mm thick, 20 teeth
8 side spacers, 0.8 mm thick
4 bottom spacers, 0.8 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Upper buffer chamber with electrodes
Lower buffer chamber
Safety Lid with power cords
4 blank glass plates
4 notched glass plates
4 clamp assemblies
2 combs, 1.5 mm thick, 15 teeth
2 combs, 1.5 mm thick, 20 teeth
8 side spacers, 1.5 mm thick
4 bottom spacers, 1.5 mm thick
2 replacement gaskets
4 replacement spring pegs
1 blocking plate for single gel operation
1 SpacerPlacer for easy spacer alignment
Twin ExW S (700-0980)
Twin L (700-1080)
as the 700-0986 but including casting base
as the 700-1205 but including casting base

4 VWR PerfectBlue Twin ver 1 10,5,2018
System Overview
PerfectBlue™ vertical dual gel systems Twin S, M, L and ExW S are characterized by sophisticated design
and durable construction. With their unique arrangement of 3 cathodes and an extended anode surrounding
the complete base of the electrophoresis chamber, electrophoretic separations of exceptional high quality
can be achieved. All of the systems are equipped with an alumina ceramic cooling system for water
circulation matching effective cooling and high migration rates without negatively affecting separation
results. Using the casting system developed for the PerfectBlue™ vertical dual gel systems up to 2 gels can
get poured at once, conveniently and without leakage. Designed for convenience, the glass plates do not
have to be re-assembled after polymerization and electrophoresis can performed straight away. This time
saving step further eliminates potential sources of error. PerfectBlue™ vertical dual gel systems are
available in 4 different formats, each one including a wide range of accessories for increased versatility. The
systems are constructed using solid parts, precisely machined from 0.6 mm thick acrylic by CNC milling. The
use of specialized adhesives neutralizes mechanical forces and thermal expansions making the system
durable and reliable. We are therefore confident of our guarantee, a full 3 year warranty on the whole range
of PerfectBlue™ electrophoresis and blotting equipment.
Technical Properties
PerfectBlue™
Cat. No.
Gel size
(W x L)
required
buffer volume
Maximal
Voltage
max. current
per gel
Typical
running time
Twin S
700-0931/
700-0937 10 x 10 cm 250 ml up to 500 V 15 - 35 mA 30 - 90 min
Twin M
700-0955/
700-0961 16 x 14 cm 650 ml up to 500 V 15 - 50 mA 60 - 120 min
Twin L
700-1080/
700-1205 20 x 20 cm 1250 ml up to 500 V 15 - 75 mA 60 - 180 min
Twin ExW S
700-0980/
700-0986 20 x 10 cm 650 ml up to 500 V 30 - 45 mA 30 - 90 min
Safety lid with power cords
Upper buffer chamber (UBC)
with side clamps and electrodes
Lower buffer chamber (LBC)
Casting base

5 VWR PerfectBlue Twin ver 1 10,5,2018
Short Instruction
Systems Overview
1. Upper buffer chamber (UBC)
with side clamps and electrodes
2. Safety lid with power cords
3. Lower buffer chamber (LBC)
4. Casting base
Leak Proof Sealing of Glass Plates
Invert casting base, so the black silicone mats face
downward and the acrylic square upward.
Place UBC on inverted casting base. Arrange gel
assemblies and place on each side of the UBC. It is
important that the bottom of the glass plates and the
spacers sit flat on the acrylic square of the inverted
casting base.
Tighten side clamps. Tighten the blocking plate in a
similar way at the other side of the UBC in case of
casting only one gel.
Invert casting base and turn out the cams.
Wet the silicone mats with e.g. water or buff
er to
prolong their life span.
Place UBC on the gaskets and turn the handles at
the same time, half a turn.
1
2
3
4

6 VWR PerfectBlue Twin ver 1 10,5,2018
Assembling the System and Electrophoresis
The gel can now be poured. Pour slowly to avoid
bubbles.
After polymerization, lift UBC from casting base and
place into the LBC. Add buffer to the UBC. Load the
samples. Add buffer to the LBC.
Place safety lid onto the unit and start the run. After the
run is completed turn off the power supply and remove
lid. Lift UBC from LBC, loosen side clamps to remove
gel assembly for further analysis.

7 VWR PerfectBlue Twin ver 1 10,5,2018
General Instructions
Leak Proof Sealing of Glass Plates
When casting acrylamide gels, we recommend using the upper buffer chamber (UBC) for the fixation of
glass plates and the casting base for plate sealing. The casting base is included in delivery or can be
purchased separately. In addition the Gel Caster is available for sealing glass plates and pouring gels while
the upper buffer chamber is used for electrophoresis. (see 'Technical Support and Ordering Information').
1. The upper buffer chamber (UBC) is attached to the lower buffer chamber (LBC) with a safety
interlocking pin and slot arrangement. The UBC may be removed for cleaning by sliding the pin out of
the slot in the center fixture of the LBC and lifting it out. For casting the gel remove the UBC and loosen
the black knobs and slide the side clamps sideways to the outermost position. The clamps will remain in
place once positioned due to the spring design.
2. Invert the casting base so that the black silicone mats face downward. Inverting the casting base is
essential for proper alignment of glass plates and spacers
before clamping them inside the UBC assuring perfect sealing
later on.
3. Place the UBC flat on the inverted casting base. Arrange a gel
assembly including a notched glass plate, side spaces and blank
glass plate onto the gasket of one side of the UBC, making sure
to place the notched plate next to the gasket (this will allow buffer
access to the gel during operation). Make sure that the bottom of
the glass plates and the spacers sit flat on the acrylic squares of
the inverted casting base. The usage of the Spacer Placer
facilitates the arrangement of the spacers. Slide the side clamps
over the assembly until the gasket of the UBC is slightly flattened
by the glass plates (do not over tighten).
Place a second gel assembly or the blocking plate on the other
side to create the upper buffer reservoir.
4. After aligning and clamping the glass plate assembly the UBC will be removed and the casting base has
to be turned around so that the black silicone mats are facing upward. Wet the silicone mats with e.g.
water or buffer to prolong their life span.

8 VWR PerfectBlue Twin ver 1 10,5,2018
5. Place the UBC onto the casting base. Insert
the casting cams (with handles pointing up)
into the holes in the UBC. Simultaneously
turn cams one half turn to tighten the
assembly onto the silicone gaskets of the
casting base. The eccentric effect of the
cams presses the UBC and therefore the
lower angles of the glass plates downward
onto the silicone mats of the casting base.
6. Once the glass plates are secured into the casting base, the
gel may be poured. Take care to avoid air bubbles while
pouring the gel. Immediately after the gel is poured insert an
appropriate comb for the creation of wells.
There is no need to apply the casting base when using pre-
cast gels. Since glass or plastic plates of pre-cast gels are
often very thin take care not to over tighten the side camps
when fixing the gel assembly into the UBC.
Assembling the System and Electrophoresis
1. To reassemble, place the upper buffer chamber (UBC) into the lower buffer chamber (LBC) on the open
side of the slot in the center fixture of the LBC. Slide the UBC towards the center of the LBC until the
pins lock into the slot. This interlocking pin and slot arrangement guarantees a tighter fit of the UBC in
the LBC and allows a reproducibility of the electric conditions.
2. For cooling through a cold water tap or recirculator chiller, flexible lab tubing can get attached to each
hose barb on the UBC marked as 'in' and 'out'. The inner diameter of the adapters of the integrated
cooling system is 6 mm, the outer diameter is 9 mm. Note the marks indicating 'in' and 'out' at the
adapters to assure the right flow direction. Water flow should not exceed 2 L per minute for the water tap
and 12 L per minute for the recirculator.

9 VWR PerfectBlue Twin ver 1 10,5,2018
3. Add electrophoresis buffer to the UBC to approximately
5 mm from the top of the gel assemblies, making sure the
level is above the gap formed by the 'ears' of the notched
plate. Be sure that no buffer is leaking from the UBC to
the LBC.
4. Remove comb and rinse wells with buffer. Load samples
onto the gel.
5. Add buffer to the LBC to approximately 2-3 mm above
the base of the gel. The open bottom end of the gel
assembly should be in contact with buffer in order for
electrophoresis to take place.
6. Set the safety lid onto the unit. Because of its asymmetrical
design this will only work in the proper orientation, connecting
the electrode of the lower buffer reservoir (typically the
anode) with the red power cord and the electrodes of the
upper buffer reservoir (typically the cathodes) with the black
power cord.
7. For cooling, turn on the water and allow it to circulate through
the system. Water flow should not exceed 2 L per minute for
the water tap and 12 L per minute for the recirculator.
8. When water has started to circulate through the system,
connect the power cords to a power supply (red to red and
black to black) and start the run by defining an appropriate
voltage or current (see 'Technical properties').
9. Turn off power supply and water after the electrophoresis is
complete.
10. Remove the lid by pushing on the acrylic thumb pegs protruding through the lid on the top of the unit.
Slide and lift UBC from LBC and drain buffer chambers separately.
11. Loosen side clamps and remove gel assemblies to further proceed with analysis.
Cleaning
Lower buffer chamber, upper buffer chamber, silicone gaskets of the casting base, glass plates, spacers and
combs should get cleaned thoroughly under running water after use. Use a mild detergent to get rid of any
debris then rinse with distilled water to avoid formation of salt spots. It is recommended to allow the chamber
to air dry rather than drying with a towel to avoid damage to the electrode wires. Each part should get
completely dry before storage.
Do not use ethanol or other organic solvents to clean acrylic products, because organic solvents cause
acrylic to 'craze' or crack!

10 VWR PerfectBlue Twin ver 1 10,5,2018
Required Materials and Recipes
Required Gel Volumes
These volumes have been calculated for glass plates and spacers available at VWR.
PerfectBlue™ Dual Gel System
Twin S
Twin M
Twin ExW S
Twin L
0.8 mm Spacer
8 ml
18 ml
16 ml
32 ml
1.5 mm Spacer
15 ml
34 ml
30 ml
60 ml
Required Buffer Volumes
PerfectBlue™ Dual Gel System
Twin S
Twin M
Twin ExW S
Twin L
250 ml
650 ml
650 ml
1250 ml
Tris-Glycine SDS-PAGE
10 x Tris-Glycine electrophoresis buffer
30 g/l Tris-Base [0.25 M]
144 g/l Glycine [1.92 M]
10 g/l SDS [1 %, w/v]
Adjust volume to 1 L using distilled H2O
Resolving gels for Tris-Glycine SDS-PAGE
6 % 8 % 10 % 12 % 15 %
H2O 5.3 4.6 4.0 3.3 2.3
30 % Acrylamide mix 2.0 2.7 3.3 4.0 5.0
1.5 M Tris-HCl (pH 8.8) 2.5 2.5 2.5 2.5 2.5
10 % SDS 0.1 0.1 0.1 0.1 0.1
10 % Ammonium persulfate (APS) 0.1 0.1 0.1 0.1 0.1
TEMED 0.008 0.006 0.004 0.004 0.004
All amounts in ml per 10 ml gel solution
5 % Stacking gels for Tris-Glycine SDS-PAGE
1 ml 2 ml 3 ml 5 ml 10 ml
H2O 0.68 1.4 2.1 3.4 6.8
30 % Acrylamide mix 0.17 0.33 0.5 0.83 1.7
1.0 M Tris-HCl (pH 6.8) 0.13 0.25 0.38 0.63 1.25
10 % SDS 0.01 0.02 0.03 0.05 0.1
10 % Ammonium persulfate (APS) 0.01 0.02 0.03 0.05 0.1
TEMED 0.001 0.002 0.003 0.005 0.01
All amounts in ml

11 VWR PerfectBlue Twin ver 1 10,5,2018
3 x Sample buffer for Tris-Glycine SDS-PAGE
1.75 ml 1 M Tris-HCl pH 6.8
1.5 ml Glycerine
5 ml SDS (10 %)
0.5 ml β-Mercaptoethanol
1.25 ml Bromophenol blue
Tris-Tricine SDS-PAGE
1 x Tris-Tricine electrophoresis buffer
Cathode buffer 12.11 g/l Tris-Base [0.1 M]
17.92 g/l Tricine [0.1 M]
1 g/l SDS [0.1 %, w/v]
Adjust volume to 1 L using distilled H2O
Anode buffer 0.2 M Tris-HCl, pH 8.9
Resolving gels for Tris-Tricine SDS-PAGE
6 % 8 % 10 % 12 %
H2O 2.1 1.4 0.6 0.0
Glycerin 1.4 1.4 1.4 1.4
30 % Acrylamide mix 2.1 2.8 3.5 4.2
3 M Tris-HCl (pH 8.45) 4.3 4.3 4.3 4.3
10 % SDS 0.1 0.1 0.1 0.1
10 % Ammonium persulfate (APS) 0.1 0.1 0.1 0.1
TEMED 0.008 0.006 0.004 0.004
All amounts in ml per 10 ml gel solution
10 % Stacking gels for Tris-Tricine SDS-PAGE
1 ml 2 ml 3 ml 5 ml 10 ml
H2O 0.03 0.06 0.08 0.16 0.32
30 % Acrylamide mix 0.32 0.65 1.0 1.62 3.24
3 M Tris-HCl (pH 8.45) 0.62 1.24 1.86 3.10 6.20
10 % SDS 0.02 0.03 0.06 0.1 0.2
10 % Ammonium persulfate (APS) 0.005 0.01 0.015 0.025 0.05
TEMED 0.001 0.001 0.002 0.005 0.01
All amounts in ml

12 VWR PerfectBlue Twin ver 1 10,5,2018
2 x Sample buffer for Tris-Tricine gels
1 ml Tris-HCl (pH 6.8)
0.8 g SDS
2.4 ml Glycerine
0.31 g DTT
2 mg Coomassie Blue G-250
Adjust volume to 10 ml using distilled H2O
Troubleshooting
Below are some possible solutions to potential problems. If these suggestions are unsuccessful, do not
hesitate to contact us (see 'Technical Support and Ordering Information').
Problem
Cause
Solution
Leakage of gel
solution
Glass plates and spacers
have not been aligned
correctly
For correct alignment of glass plate sandwich it is
necessary to pla
ce the upper buffer chamber on top of the
inverted casting base. The bottom of the glass plates and
the spacers need to sit flat on the acrylic squares.
Glass plates or spacers are
dirty
Clean thoroughly.
Uneven edges of glass
plates
Only use glass plates with polished edges.
Silicone gaskets of casting
base are dirty
Clean thoroughly with a mild detergent and rinse with
distilled water, dry without smearing, i.e. hand dry with a
lint free towel.
Silicone gaskets of casting
base are brittle or worn out
Replace silicone gaskets which can be easily removed.
New gaskets will stick due to their own adherence. Also,
wet silicone mats with e.g. water or buffer before placing
UBC onto the casting base to prolong their life span.
Upper buffer
chamber is
leaking
Gaskets of the upper buffer
chamber are deformed, too
old or dirty
Remove gasket, wash in warm water to remove excess
salts, and place the gasket back in the groove. If the
gaskets are too old or brittle, they should be replaced. If
the clamps have been over tightened in the past, the
gasket can be pushed too far into the gasket groove and
will not make a seal.
Leakage of buffer through
gel sides/spacers
Do not move spacers after the gel polymerized. Make sure
that the glass plates are seated firmly against the gasket.
Always use combs and spacers of the same thickness.
3 x Sample buffer for native gel electrophoresis
4.5 ml Glycerine
0.31 g Bis-Tris-Base
adjust to pH 7 with HCL
Adjust volume to 10 ml using distilled H2O

13 VWR PerfectBlue Twin ver 1 10,5,2018
Problem
Cause
Solution
Run time is too
long
Buffer is too dilute
After a certain running time the ions might be exhausted,
so the electric resistance increases and the current
decreases at constant voltage, so that the maximum
voltage is not enough to keep the current constant. Check
buffer recipe and try again with new buffer. Check if
voltage and current of the different runs is the same.
Upper buffer chamber is
leaking
See above 'Upper buffer chamber is leaking'.
Running at too low current or
voltage
Make sure you are running using the suggested running
conditions for this unit (see 'Technical properties'). When
running at constant current, the current value is per gel.
Running too fast
Buffers are too concentrated
Check buffer recipe; remake and try again. If voltage is
lower than usual when running at constant current, the
buffer is probably too concentrated.
Voltage or current set too
high.
Turn down electrical settings.
Smiling of dye
front
Center of gel is running
hotter than the ends
Use the cooling option of the unit. Turn down the electrical
settings.
Bands spreading
outwards
Diffusion of sample when
loading
Make sure that the samples are loaded quickly and the
power is applied as soon as possible after loading.
Diffusion of sample during
run in the stacking gel
Increase percentage of stacking gel or increase current by
25 % when stacking.
Lower ionic strength of
sample
Match the ionic strength of sample with that of the gel.
Bands are
narrower than
sample wells
Ionic strength of sample is
higher than of the gel
De-salt the sample or use sample buffer of the same ionic
strength as the gel.
Broad lanes at
bottom of gel
Will occur when adjoining
lanes are loaded with
samples with different
buffers. Normal in gradient
gels.
Use the same sample buffer for all of the samples loaded
per gel.
Skewed bands
Gel has not polymerized
properly at wells.
De-gas gel solution before casting and increase APS and
TEMED concentrations. The comb can be wiped with
TEMED just prior to casting to improve polymerization.
Salt concentration is too
high in sample.
Desalt the samples by dialysis, desalting columns or
precipitation/resolving.
Resolving gel is uneven at
top.
Overlay gel carefully using water saturated n-butanol and
make sure casting stand is level.
The upper buffer chamber
is leaking either through the
gel or along the side
spacers.
Check gel to make sure that it is a solid slab inside the
glass and check the setup of the apparatus to ensure a
seal with the gasket. Always use combs and spacers of
the same thickness.

14 VWR PerfectBlue Twin ver 1 10,5,2018
Problem
Cause
Solution
Streaked bands
Overloading of sample.
Use less protein or sample when next loading.
Sample has precipitated.
Centrifuge sample before adding sample buffer or use a
lower percentage acrylamide gel.
'Frowning' of
outside lanes
Leakage of buffer along
sides or along spacers
inside the gel assembly.
Do not move spacer after polymerization of gel. Make
sure that the glass plates are pressed firmly against the
gasket of the UBC.
Silicone mats
wear out quickly
Sticky surface of the mats
adheres too strongly to the
glass plates. Upon removal
of UBC silicone mats are
torn apart.
Wet the silicone mats with e.g. water or buffer before
placing UBC onto the casting base to prolong their life
span.
Double bands
('doublets')
Due to re-oxidation or
insufficient reduction of the
sample.
If using a reducing agent, prepare fresh sample buffer
every 30 days. Increase the concentration of 2-
mercaptoethanol or dithiothreitol in the sample.
Technical Support and Ordering Information
For technical questions and more detailed information on VWR’s products please contact your local VWR
office or visit the VWR website at vwr.com.
Visit the VWR website at vwr.com for:
• Complete technical service contact information
• Access to the VWR Online Catalogue, and information about accessories and related products
• Additional product information and special offers
PerfectBlue™ Dual Gel System Twin S
Item
Description
Cat. No.
Dual Gel System Twin S
system for gels 10 x 10 cm (W x L), without casting base
700-0937
system for gels 10 x 10 cm (W x L), with casting base
700-0931
Casting base S
including silicone gaskets and cams
700-0932
Gel Caster S
complete system for sealing up to 2 gels
700-0935
Silicone gaskets
2 replacement silicone gaskets for Casting base S
700-0933
Replacement gaskets
2 replacement gaskets for upper buffer chamber S
700-0936
Side clamps
2 side clamps for fixation of glass plates
700-0934
Wing screws
2 wing screws for fixation of side clamps
700-0944
Notched glass plate
clear, 10 x 10 x 0.24 cm
700-0920
Blank glass plate
clear, 10 x 10 x 0.24 cm
700-0919
Notched alumina plate
white, 10 x 10 x 0.10 cm
700-0921
Standard combs
1.5 mm
6 teeth
230 µl*
700-0927
1.5 mm
8 teeth
160 µl*
700-0929
1.5 mm
10 teeth
120 µl*
700-0918
1.5 mm
12 teeth
90 µl*
700-0923
0.8 mm
6 teeth
120 µl*
700-0926
0.8 mm
8 teeth
85 µl*
700-0928

15 VWR PerfectBlue Twin ver 1 10,5,2018
0.8 mm
10 teeth
60 µl*
700-0917
0.8 mm
12 teeth
45 µl*
700-0922
Preparative combs
1.5 mm
2 teeth
1500/100 µl*
700-0939
0.8 mm
2 teeth
800/60 µl*
700-0938
SpacerPlacer
Facilitates the correct alignment of Spacers
700-0942
Spacer Sets
(2 side & 1 bottom spacer)
1.5 mm
0.7 cm wide
3 pieces
700-0941
0.8 mm
0.7 cm wide
3 pieces
700-0940
* volumes are calculated for a well length of 14 mm
PerfectBlue™ Dual Gel System Twin M
Item
Description
Cat. No.
Dual Gel System Twin M
system for gels 16 x 14 cm (W x L), without casting base
700-0961
system for gels 16 x 14 cm (W x L), with casting base
700-0955
Casting base M
including silicone gaskets and cams
700-0956
Gel Caster M
complete system for sealing up to 2 gels
700-0959
Silicone gaskets
2 replacement silicone gaskets for Casting base M
700-0957
Replacement gaskets
2 replacement gaskets for upper buffer chamber M
700-0960
Side clamps
2 side clamps for fixation of glass plates
700-0958
Wing screws
2 wing screws for fixation of side clamps
700-0944
Notched glass plate
clear, 16 x 14 x 0.32 cm
700-0948
Blank glass plate
clear, 16 x 14 x 0.32 cm
700-0947
Standard combs
1.5 mm
10 teeth
310 µl*
700-0946
1.5 mm
15 teeth
180 µl*
700-0950
1.5 mm
20 teeth
115 µl*
700-0952
1.5 mm
24 teeth
85 µl*
700-0954
0.8 mm
10 teeth
165 µl*
700-0945
0.8 mm
15 teeth
95 µl*
700-0949
0.8 mm
20 teeth
60 µl*
700-0951
0.8 mm
24 teeth
45 µl*
700-0953
Preparative combs
1.5 mm
2 teeth
3900/155 µl*
700-0963
0.8 mm
2 teeth
2095/80 µl*
700-0962
SpacerPlacer
Facilitates the correct alignment of Spacers
700-0966
Spacer Sets
(2 side & 1 bottom spacer)
1.5 mm
1.0 cm wide
3 pieces
700-0965
0.8 mm
1.0 cm wide
3 pieces
700-0964
* volumes are calculated for a well length of 20 mm
PerfectBlue™ Dual Gel System Twin L
Item
Description
Cat. No.
Dual Gel System Twin L
system for gels 20 x 20 cm (W x L), without casting base
700-1205
system for gels 20 x 20 cm (W x L), with casting base
700-1080
Casting base L
including silicone gaskets and cams
700-1087
Gel Caster L
complete system for sealing up to 2 gels
700-1093

16 VWR PerfectBlue Twin ver 1 10,5,2018
Silicone gaskets
2 replacement silicone gaskets for Casting base L
700-1089
Replacement gaskets
2 replacement gaskets for upper buffer chamber L
700-1098
Side clamps
2 side clamps for fixation of glass plates
700-1092
Wing screws
2 wing screws for fixation of side clamps
700-0944
Notched glass plate
clear, 20 x 20 x 0.32 cm
700-1034
Blank glass plate
clear, 20 x 20 x 0.32 cm
700-1020
Standard combs
1.5 mm
10 teeth
405 µl*
700-0999
1.5 mm
15 teeth
245 µl*
700-1014
1.5 mm
20 teeth
165 µl*
700-1017
1.5 mm
25 teeth
115 µl*
700-1046
0.8 mm
10 teeth
215 µl*
700-0998
0.8 mm
15 teeth
130 µl*
700-1013
0.8 mm
20 teeth
85 µl*
700-1015
0.8 mm
25 teeth
60 µl*
700-1043
Preparative combs
1.5 mm
2 teeth
4915/155 µl*
700-1210
0.8 mm
2 teeth
2620/80 µl*
700-1209
SpacerPlacer
Facilitates the correct alignment of Spacers
700-1215
Spacer Sets
(2 side & 1 bottom spacer)
1.5 mm
1.0 cm wide
3 pieces
700-1214
0.8 mm
1.0 cm wide
3 pieces
700-1211
* volumes are calculated for a well length of 20 mm
PerfectBlue™ Dual Gel System Twin ExW S
Item
Description
Cat. No.
Dual Gel System
Twin ExW S
system for gels 20 x 10 cm (W x L), without casting
base
700-0986
system for gels 20 x 10 cm (W x L), with casting base
700-0980
Casting base ExW
including silicone gaskets and cams
700-0981
Gel Caster ExW S
complete system for sealing up to 2 gels
700-0984
Silicone gaskets
2 replacement silicone gaskets for Casting base ExW S
700-0982
Replacement gaskets
2 replacement gaskets for upper buffer chamber ExW S
700-0985
Side clamps
2 side clamps for fixation of glass plates
700-0983
Wing screws
2 wing screws for fixation of side clamps
700-0944
Notched glass plate
clear, 20 x 10 x 0.30 cm
700-0971
Blank glass plate
clear, 20 x 10 x 0.30 cm
700-0970
Standard combs
1.5 mm
10 teeth
285 µl*
700-0969
1.5 mm
15 teeth
170 µl*
700-0974
1.5 mm
20 teeth
115 µl*
700-0976
1.5 mm
25 teeth
80 µl*
700-0978
0.8 mm
10 teeth
150 µl*
700-0968
0.8 mm
15 teeth
90 µl*
700-0973
0.8 mm
20 teeth
60 µl*
700-0975
0.8 mm
25 teeth
40 µl*
700-0977

17 VWR PerfectBlue Twin ver 1 10,5,2018
Micro titer combs
1.5 mm
18 teeth
135 µl*
700-0991
1.5 mm
36 teeth
55 µl*
700-0989
0.8 mm
18 teeth
70 µl*
700-0990
0.8 mm
36 teeth
30 µl*
700-0988
Preparative combs
1.5 mm
2 teeth
3575/110 µl*
700-0993
0.8 mm
2 teeth
1905/60 µl*
700-0992
SpacerPlacer
Facilitates the correct alignment of Spacers
700-0996
Spacer Sets
(2 side & 1 bottom spacer)
1.5 mm
1.2 cm wide
3 pieces
700-0995
0.8 mm
1.2 cm wide
3 pieces
700-0994
* volumes are calculated for a well length of 14 mm
Power Supplies
Do not hesitate to contact us for advice on which Power Supply is most suitable for your application.

18 VWR PerfectBlue Twin ver 1 10,5,2018
Your Distributor
Austria
VWR International GmbH
Graumanngasse 7
1150 Vienna
Tel.: [phone]
Email: [e-mail]
Belgium
VWR International bvba
Researchpark Haasrode 2020
Geldenaaksebaan 464
3001 Leuven
Tel.: [phone]
Email: [e-mail]
China
VWR International China Co., Ltd
Shanghai Branch
Room 256, No. 3058 Pusan Road
Pudong New District
Shanghai 200123
Tel.:[phone]
Fax:[phone]
Email: [e-mail]
Czech Republic
VWR International s. r. o.
Veetee Business Park
Pražská 442
CZ - 281 67 Stříbrná Skalice
Tel.: [phone]
Email: [email protected]
Denmark
VWR International A/S
Tobaksvejen 21
2860 Søborg
Tel.: [phone]
Email: [e-mail]
Finland
VWR International Oy
Valimotie 9
00380 Helsinki
Tel.:[phone]
Email: [e-mail]
France
VWR International S.A.S.
Le Périgares – Bâtiment B
201, rue Carnot
94126 Fontenay-sous-Bois cedex
Tel.: [phone]* (national)
Tel.: [phone]
(international)
Email: [e-mail]
* 0,18 € TTC/min
Germany
VWR International GmbH
Hilpertstraße 20a
D - 64295 Darmstadt
Freecall: 0800 702 00 07
Tel.: [phone]
(international)
Email: [e-mail]
Hungary
VWR International Kft.
Simon László u. 4.
4034 Debrecen
Tel.: [phone]
Email: [e-mail]
India
VWR Lab Products Private Limited
No.139. BDA Industrial Suburb,
6th Main, Tumkur Road, Peenya
Post,
Bangalore, India – 560058
Tel.: [phone]
Email: [e-mail]
Ireland / Northern Ireland
VWR International Ltd / VWR
International (Northern Ireland) Ltd
Orion Business Campus
Northwest Business Park
Ballycoolin
Dublin 15
Tel.: [phone]
Email [e-mail]
Italy
VWR International S.r.l.
Via San Giusto 85
20153 Milano (MI)
Tel.: [phone]
Email: [e-mail]
The Netherlands
VWR International B.V.
Postbus 8198
1005 AD Amsterdam
Tel.: [phone]
Email: [e-mail]
Norway
VWR International AS
Haavard Martinsens vei 30
0978 Oslo
Tel.: [phone]
Email: [e-mail]
Poland
VWR International Sp. z o.o.
Limbowa 5
80-175 Gdansk
Tel.: [phone]
Email: [e-mail]
Portugal
VWR International –
Material de Laboratório, Lda
Centro Empresarial de Alfragide
Rua da Indústria, nº 6
2610-088 Alfragide
Tel.: [phone]
Email: [e-mail]
Singapore
VWR Singapore Pte Ltd
18 Gul Drive
Singapore 629468
Tel: [phone]
Email: [e-mail]
Spain
VWR International Eurolab S.L.
C/ Tecnología 5-17
A-7 Llinars Park
08450 - Llinars del Vallès
Barcelona
Tel.: [phone]
Email: [e-mail]
Sweden
VWR International AB
Fagerstagatan 18a
163 94 Stockholm
Tel.: [phone]
Email: [e-mail]
Switzerland
VWR International AG
Lerzenstrasse 16/18
8953 Dietikon
Tel.: [phone]
Email: [e-mail]
UK
VWR International Ltd
Customer Service Centre
Hunter Boulevard
Magna Park
Lutterworth
Leicestershire
LE17 4XN
Tel.: [phone]
Email: [e-mail]