Zeiss ApoTome.2 User manual

Operating Manual
ApoTome.2

Carl Zeiss Copyright ApoTome.2
2 423667-7144-001 05/2012
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
microscop[email protected]m
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Number of this manual: 423667-7144-001
Date of issue: Version 3 May 01, 2012

ApoTome.2 Contents Carl Zeiss
05/2012 423667-7144-001 3
CONTENTS
Page
1INTRODUCTION................................................................................................................ 6
1.1 Important notes.................................................................................................................. 6
1.2 Notes on device safety........................................................................................................ 7
1.3 Warning and information labels ........................................................................................ 10
1.4 Notes on warranty............................................................................................................ 10
2INSTRUMENT DESCRIPTION........................................................................................... 11
2.1 Product name and intended use........................................................................................ 11
2.2 Instrument description and main features.......................................................................... 11
2.3 System overview............................................................................................................... 12
2.3.1 ApoTome.2 for Axio Imager.............................................................................................. 12
2.3.2 ApoTome.2 for Axio Observer and Axiovert 200................................................................ 13
2.3.3 ApoTome.2 for Axio Zoom.V16 ........................................................................................ 14
2.4 General view of ApoTome.2 ............................................................................................. 15
2.5 Operating principle........................................................................................................... 16
2.6 Suitability of objectives for the use of ApoTome.2 ............................................................. 17
2.7 Technical data .................................................................................................................. 21
3STARTUP......................................................................................................................... 23
3.1 Microscope stand requirements ........................................................................................ 23
3.2 Unpacking ApoTome.2..................................................................................................... 23
3.3 Connecting the control box .............................................................................................. 24
4OPERATION .................................................................................................................... 25
4.1 Control and functional elements of ApoTome.2 ................................................................ 25
4.2 Switching ApoTome.2 on/off ............................................................................................ 26
4.2.1 Switching on .................................................................................................................... 26
4.2.2 Switching off.................................................................................................................... 26
4.3 Operating the slider.......................................................................................................... 26
4.3.1 Inserting the slider ............................................................................................................ 26
4.3.2 Click-stop position 1......................................................................................................... 26
4.3.3 Click-stop position 2......................................................................................................... 26
5CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ......................................... 27
5.1 Cleaning the device .......................................................................................................... 27
5.2 Maintaining the device ..................................................................................................... 28
5.2.1 Performing checks............................................................................................................ 28
5.2.2 Replacing fuses................................................................................................................. 28
5.3 Troubleshooting ............................................................................................................... 29
5.4 Wear parts and tools ........................................................................................................ 29
5.5 Requesting service............................................................................................................ 30

Carl Zeiss Contents ApoTome.2
4 423667-7144-001 05/2012
6APPENDIX .......................................................................................................................31
6.1 List of abbreviations ..........................................................................................................31
6.2 Index ................................................................................................................................32

ApoTome.2 List of figures Carl Zeiss
05/2012 423667-7144-001 5
LIST OF FIGURES
Page
Fig. 1-1 Warning and information labels ........................................................................................ 10
Fig. 2-1 System overview ApoTome.2 for Axio Imager.................................................................... 12
Fig. 2-2 System overview ApoTome.2 for Axio Observer and Axiovert 200 ...................................... 13
Fig. 2-3 System overview ApoTome.2 for Axio Zoom.V16............................................................... 14
Fig. 2-4 Components of ApoTome.2.............................................................................................. 15
Fig. 2-5 Changer with transmission grids ....................................................................................... 16
Fig. 2-6 Image capture in three defined steps (phases) ................................................................... 16
Fig. 3-1 Connectors on control box (rear panel) ............................................................................. 24
Fig. 4-1 Control and functional elements of ApoTome.2 ................................................................ 25
Fig. 5-1 Replacing fuses................................................................................................................. 28

INTRODUCTION
Carl Zeiss Important notes ApoTome.2
6 423667-7144-001 05/2012
1INTRODUCTION
1.1 Important notes
The ApoTome.2 system is an extension to the Axio Imager, Axio Observer, Axiovert
200 and
Axio
Zoom.V16 microscopes. The ApoTome.2 system consists of a slider, which is inserted in
the plane of the luminous-field diaphragm of the incident-
light beam path, and an
accompanying control box.
A complete ApoTome.2 imaging system comprises the following components:
•microscope
•anti-vibration table or anti-vibration microscope support
•cooled digital camera with high dynamic range
•PC with at least 1
GB RAM according to Carl Zeiss specifications plus monitor, operating
system Windows®XP, Windows® Vista (32 bit version) or Windows®7 (32 bit version)
•ApoTome.2 slider with ApoTome control box
•Imaging software AxioVision 4.8.2 control software, service pack 1 / ZEN 2011 (blue edition)
This Operating Manual describes the
use of the ApoTome.2 hardware. For notes and
instructions for the control of the ApoTome.2 through software, please refer to the Operating
Manual of the imaging software AxioVision / ZEN 2011 (blue edition).
Prior to the initial start-up of the ApoTome
.2, read the notes on instrument safety as well as
the chapters Instrument Description (Chapter 2) and Startup (Chapter 3).
Especially note that on stands of the Axiovert series delivered before May 1, 2002 the base
plate of the fluorescence beam path must be replaced by a new base plate (000000-1197-874).

INTRODUCTION
ApoTome.2 Notes on device safety Carl Zeiss
05/2012 423667-7144-001 7
1.2 Notes on device safety
The ApoTome.2 was designed, produced and tested in compliance with DIN EN 61010-1 (IEC 61010-1)
"Safety requirements for electrical measuring, control and laboratory instruments" and meets
the requirements of the EC directives 2006/95/EC "Low Voltage Directive" and 2004/108/EC
"Electromagnetic Compatibility".
The device is to be deposed of according to the WEEE Directive 2002/96/EC.
It is marked with the mark.
This Operating Manual includes information and warnings that must be observed by the user.
The following warning and information symbols are used in this operating manual:
CAUTION
This symbol is a warning that indicates a hazard to the user of the instrument.
CAUTION
Hot surface!
CAUTION
Emitted LED radiation!
CAUTION
Disconnect the instrument from line power before opening it!
CAUTION
This symbol is a warning that indicates a hazard to the user, the instrument or instrument
system.
NOTE
This symbol is a warning that you must observe under all circumstances.
The ApoTome.2 may only be used for the techniques described in this manual.
Particularly observe the following notes:
The manufacturer cannot assume any liability for any other application,
including that of
individual modules or single parts. This also applies to all service or repair work that is not
carried out by authorized service personnel. Moreover, all warranty claims shall be forfeited.
The power plug must be inserted in an outle
t featuring a grounding (earth) contact. The
grounding effect must not be made ineffective by an extension cable that does not have a
protective ground wire.

INTRODUCTION
Carl Zeiss Notes on device safety ApoTome.2
8 423667-7144-001 05/2012
If it is determined that protection measures are no longer effective, the instrument must be
sw
itched off and safeguarded against inadvertent operation. Please contact the Zeiss Service
department or Carl Zeiss Microscopy Service to repair the instrument.
The ApoTome.2 control box is equipped with an integrated power supply which permits the
use of line voltages in the range between 100 and 240 V ±10 %, 50 / 60 Hz, without having to
adjust the line voltage on the instrument.
Before switching on the instrument, check whether it is suitable for the line voltage present.
Always disconnect
the instrument from the power outlet before opening it and before
changing the fuses.
Make sure to use only fuses of the rated power required. The use of makeshift fuses and the
short-circuiting of the fuse holders are not permitted.
The ApoTome.2 s
lider and the ApoTome.2 control box are not equipped with any special
devices for the protection from corrosive, potentially infectious, toxic, and radioactive
specimens or other specimens that could be hazardous to health. When handling such
specimens, ob
serve all legal regulations, particularly the relevant national regulations for the
prevention of accidents.
Gas-discharge lamps, e.g. HBO 100, emit ultraviolet radiation that can cause burns to the eyes
and skin. Therefore, never look directly into the light of these lamps and avoid direct incidence
of their light on your skin. When using the microscope, always use the protective units
belonging to the instrument (e.g. special attenuation filters or the fluorescence shield). When
they are hot, gas-di
scharge lamps are under high internal pressure. Therefore, replace them
only when they have cooled down and make sure to wear protective gloves and a face guard.
(For details, please refer to Operating Manual HBO 100.)
When using fluorescence filters, do not remove the heat-
absorbing filter for the protection
against heat emitted by the microscope illuminator since fluorescence filters are sensitive to
heat and their performance could be impaired.
Avoid touching the hot lamp housing. Always pull the power plug before replacing the lamp
and allow the instrument to cool down for approximately 15 minutes.
Do not operate the device in potentially explosive environments.

INTRODUCTION
ApoTome.2 Notes on device safety Carl Zeiss
05/2012 423667-7144-001 9
Dust and dirt can impair the performance of the instrument. Therefore, prot
ect the instrument
from these influences as far as possible and cover it with the dust cover when not in use.
Always check whether the instrument is switched off before you cover it.
Clogging or covering ventilation slots can lead to heat accumulation
that can damage the
instrument and, in extreme cases, cause fire. Always keep the ventilation slots clear and make
sure that no objects enter the instrument through these slots.
The instruments may only be operated by trained personnel who must be aware of the possible
dangers involved with microscopy and the particular application concerned. The ApoTome.2 is
a high-
precision instrument that can be impaired in its performance or destroyed when handled
improperly.
Do not put defective instrumen
ts in household waste. They have to be disposed of in
accordance with the WEEE Directive 2002/96/EC.
Specimens also need to be properly disposed of in conformity with applicable legal provisions
and in-house work instructions.

INTRODUCTION
Carl Zeiss Warning and information labels ApoTome.2
10 423667-7144-001 05/2012
1.3 Warning and information labels
Fig. 1-1 Warning and information labels
1.4 Notes on warranty
The manufacturer guarantees that the instrument has no material and production defects when
delivered. You must inform us of any defects immediately and minimize any damage. If the manufacturer
is informed of such a defect, he is obligated to remove it; it is his decision whether he does this by
repairing the instrument or by delivering an instrument free of any defect. No guarantee is provided for
defects caused by natural wear (wearing parts in particular) and improper use.
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other
tampering with the instrument, particularly the removal or replacement of instrument components, or
the use of accessories from other manufacturers. This forfeits all warranty claims.
With the exception of the work specified in this manual, no maintenance or repair of the ApoTome.2
may be undertaken. Repairs may only be performed by Zeiss Service staff or specially authorized
personnel. Should any defect occur with the instrument, please get in touch first with the
Carl Zeiss Microscopy Service in Germany (see page 30) or with the Carl Zeiss agency in your country.

INSTRUMENT DESCRIPTION
ApoTome.2 Product name and intended use Carl Zeiss
05/2012 423667-7144-001 11
2INSTRUMENT DESCRIPTION
2.1 Product name and intended use
Manufacturer's product name: ApoTome.2 for Axio Imager (423667-9100-000)
ApoTome.2 for Axio Observer and Axiovert 200 (423667-9000-000)
ApoTome.2 for Axio Zoom.V16 (423667-9200-000)
The ApoTome.2 allows depth-discriminated images (= optical sections) of fluorescence specimens to be
produced. Compared to the conventional reflected light fluorescence methods, these optical sections
feature increased contrast and enhanced optical resolution in axial direction. Furthermore, optical
sections through the specimen are the prerequisite for the three-dimensional reconstruction of structures.
2.2 Instrument description and main features
The ApoTome.2 hardware consists of two components:
1. slider with transmission grids changer
2. control box
The slider is directly connected to the control box via a cable. The control box is connected to the PC by a
USB cable or, optionally, it can be connected directly to the microscope by a CAN BUS cable. In this case,
the ApoTome.2 directly communicates with the PC via the electronic system of the microscope.
If an Axiovert 200 is used, the control box can only be connected to the PC by a USB cable. A
connection via CAN BUS is not possible!
Major features of the ApoTome.2 include:
−ApoTome.2 slider for the plane of the luminous-field diaphragm in the reflected light path
−ApoTome.2 slider with two click-stop positions:
Click-stop position 1:
An open passage is provided in the reflected light path. Thus, normal fluorescence observations can be
carried out in the wide field, e.g. to find interesting structures and to position the specimens.
Click-stop position 2:
In this position, a glass plate with evaporated grid structure is in the light path. The grid structure is
laterally moved in the specimen plane by means of a scanner mechanism. By capturing three (or more)
images at different grid positions and by subsequent calculation, it is possible to produce an optical
section through the specimen. Three different grid frequencies selected via software are available.
They allow the user to obtain different section thicknesses and to use different objectives.

INSTRUMENT DESCRIPTION
Carl Zeiss System overview ApoTome.2
12 423667-7144-001 05/2012
2.3 System overview
2.3.1 ApoTome.2 for Axio Imager
Fig. 2-1 System overview ApoTome.2 for Axio Imager

INSTRUMENT DESCRIPTION
ApoTome.2 System overview Carl Zeiss
05/2012 423667-7144-001 13
2.3.2 ApoTome.2 for Axio Observer and Axiovert 200
Fig. 2-2 System overview ApoTome.2 for Axio Observer and Axiovert 200

INSTRUMENT DESCRIPTION
Carl Zeiss System overview ApoTome.2
14 423667-7144-001 05/2012
2.3.3 ApoTome.2 for Axio Zoom.V16
Fig. 2-3 System overview ApoTome.2 for Axio Zoom.V16

INSTRUMENT DESCRIPTION
ApoTome.2 General view of ApoTome.2 Carl Zeiss
05/2012 423667-7144-001 15
2.4 General view of ApoTome.2
1Slider ApoTome.2 for Axio Imager
2Slider ApoTome.2 for Axio Observer and Axiovert 200
3Slider ApoTome.2 for Axio Zoom.V16
4Control box ApoTome.2
Fig. 2-4 Components of ApoTome.2

INSTRUMENT DESCRIPTION
Carl Zeiss Operating principle ApoTome.2
16 423667-7144-001 05/2012
2.5 Operating principle
Three different transmission grids (Fig. 2-5/1) are
installed in the slider ApoTome.2 on a motorized
changer (Fig. 2-5/2). The transmission grids are
three metallic grid structures that are evaporated
on a glass plate.
If the transmission grid is located in the
illumination beam path, the grid structure will be
imaged in the specimen plane. For image capture
the image of the grid structure is shifted in three
defined steps ("phases") in the specimen by means
of a scanner mechanism in ApoTome.2. During this
procedure a given point of the specimen is
illuminated with different intensity depending on
the phase position of the transmission grid.
The three images captured (see Fig. 2-6) with the grid structures are calculated, so that an optical section
with increased contrast and depth discrimination through the specimen will be obtained. The calculation
makes the grid structures of the initial images disappear.
Fig. 2-6 Image capture in three defined steps (phases)
The ApoTome.2 slider is provided with three different transmission grids, which cover the total
magnification range, as indicated in Chapter 2.6. The software automatically recommends the optimal
grid to be used. Optionally, another grid can be selected manually (e.g. to adjust the section thickness to
the experimental conditions).
Please refer to Chapter 2.6 for a precise list about the correct transmission grids for the different
magnifications.
Fig. 2-5 Changer with transmission grids

INSTRUMENT DESCRIPTION
ApoTome.2 Suitability of objectives for the use of ApoTome.2 Carl Zeiss
05/2012 423667-7144-001 17
2.6 Suitability of objectives for the use of ApoTome.2
The operating principle described in Chapter 2.5 places specific demands on the correction state of the
objectives used.
Especially the correction of the longitudinal color aberration must be of high quality. Shifting the grid
structure along the optical axis minimizes the residual error.
The recommended objectives for the Axio Imager or Axio Observer / Axiovert 200 or Axio Zoom.V16
microscopes are listed in the following tables.
Axio Imager
Objective M NA Immersion Grid / Section thickness
@ 490nm [RU/µm]
DAPI with
FS 34
DAPI with
FS 49
High grid Middle grid Low grid
EC Plan-Neofluar 10x 0,3 Air 2,9 / 31,9 1,7 / 18,2 0,9 / 9,9


EC Plan-Neofluar 20x 0,5 Air 2,4 / 9,2 1,4 / 5,3 0,7 / 2,9


EC Plan-Neofluar 40x 0,75 Air 1,6 / 2,8 0,9 / 1,6 0,5 / 0,9


EC Plan-Neofluar 40x 1,3 Oil 2,5 / 2,2 1,4 / 1,2 0,8 / 0,7


EC Plan-Neofluar 63x 0,95 Air 1,0 / 1,1 0,6 / 0,7 0,4 / 0,4


EC Plan-Neofluar 63x 1,25 Oil 1,6 / 1,5 0,9 / 0,9 0,5 / 0,5


EC Plan-Neofluar 100x 1,3 Oil 1,0 / 0,9 0,6 / 0,5 0,4 / 0,3


LCI Plan-Neofluar 25x 0,8 Oil, water or
glycerin 2,9 / 6,6 1,7 / 3,7 0,9 / 2,0


LCI Plan-Neofluar 63x 1,3
Water or
glycerin 1,5 / 1,3 0,9 / 0,7 0,5 / 0,4


Plan-Apochromat 10x 0,45 Air 4,2 / 20,4 2,4 / 11,5 1,3 / 6,2


Plan-Apochromat 20x 0,8 Air 3,2 / 4,9 1,8 / 2,8 1,0 / 1,5


Plan-Apochromat 40x 0,95 Air 1,6 / 1,7 0,9 / 1,0 0,5 / 0,5


Plan-Apochromat 40x 1,3 Oil 2,5 / 2,2 1,4 / 1,2 0,8 / 0,7


Plan-Apochromat 40x 1,4 Oil 2,4 / 1,8 1,4 / 1,0 0,7 / 0,6


Plan-Apochromat 63x 1,4 Oil 1,6 / 1,2 0,9 / 0,7 0,5 / 0,4


Plan-Apochromat 100x 1,4 Oil 1,0 / 0,8 0,6 / 0,5 0,4 / 0,3


LD LCI Plan-
Apochromat 25x 0,8 Oil, water or
glycerin 2,9 / 6,6 1,7 / 3,7 0,9 / 2,0


C-Apochromat 10x 0,45 Water 4,2 / 20,4 2,4 / 11,5 1,3 / 6,2


C-Apochromat 40x 1,2 Water 2,2 / 2,0 1,2 / 1,1 0,7 / 0,6


C-Apochromat 63x 1,2 Water 1,4 / 1,3 0,8 / 0,7 0,5 / 0,4


LD C-Apochromat 40x 1,1 Water 2,2 / 2,3 1,2 / 1,3 0,7 / 0,7


α
Plan-Apochromat 63x 1,46 Oil 1,5 / 1,0 0,9 / 0,6 0,5 / 0,3


α
Plan-Fluar 100x 1,45 Oil 1,0 / 0,7 0,6 / 0,4 0,3 / 0,2


α
Plan-Apochromat 100x 1,46 Oil 1,0 / 0,7 0,6 / 0,4 0,3 / 0,2



INSTRUMENT DESCRIPTION
Carl Zeiss Suitability of objectives for the use of ApoTome.2 ApoTome.2
18 423667-7144-001 05/2012
Axio Observer / Axiovert 200
Objective M NA Immersion Grid / Section thickness
@ 490nm [RU/µm]
DAPI with
FS 34
DAPI with
FS 49
High grid Middle grid Low grid
EC Plan-Neofluar 10x 0,3 Air 2,9 / 31,5 1,7 / 18,5 0,9 / 9,8


EC Plan-Neofluar 20x 0,5 Air 2,3 / 9,0 1,4 / 5,4 0,7 / 2,9


EC Plan-Neofluar 40x 0,75 Air 1,6 / 2,7 0,9 / 1,6 0,5 / 0,9


EC Plan-Neofluar 40x 1,3 Oil 2,4 / 2,1 1,4 / 1,3 0,8 / 0,7


EC Plan-Neofluar 63x 0,95 Air 1,0 / 1,1 0,6 / 0,7 0,4 / 0,4


EC Plan-Neofluar 63x 1,25 Oil 1,6 / 1,5 0,9 / 0,9 0,5 / 0,5


EC Plan-Neofluar 100x 1,3 Oil 1,0 / 0,9 0,6 / 0,6 0,4 / 0,3


LCI Plan-Neofluar 25x 0,8 Oil, water or
glycerin 2,9 / 6,5 1,7 / 3,8 0,9 / 2,0


LCI Plan-Neofluar 63x 1,3 Water or
glycerin 1,5 / 1,3 0,9 / 0,8 0,5 / 0,4


Plan-Apochromat 10x 0,45 Air 4,2 / 20,2 2,4 / 11,7 1,3 / 6,1


Plan-Apochromat 20x 0,8 Air 3,1 / 4,8 1,8 / 2,8 1,0 / 1,5


Plan-Apochromat 40x 0,95 Air 1,6 / 1,7 0,9 / 1,0 0,5 / 0,5


Plan-Apochromat 40x 1,3 Oil 2,4 / 2,2 1,4 / 1,3 0,8 / 0,7


Plan-Apochromat 40x 1,4 Oil 2,4 / 1,8 1,4 / 1,1 0,7 / 0,6


Plan-Apochromat 63x 1,4 Oil 1,5 / 1,2 0,9 / 0,7 0,5 / 0,4


Plan-Apochromat 100x 1,4 Oil 1,0 / 0,8 0,6 / 0,5 0,4 / 0,3


LD LCI Plan-
Apochromat 25x 0,8 Oil, water or
glycerin 2,9 / 6,5 1,7 / 3,8 0,9 / 2,0


C-Apochromat 10x 0,45 Water 4,2 / 20,2 2,4 / 11,7 1,3 / 6,1


C-Apochromat 40x 1,2 Water 2,1 / 1,9 1,3 / 1,1 0,7 / 0,6


C-Apochromat 63x 1,2 Water 1,4 / 1,3 0,8 / 0,7 0,5 / 0,4


LD C-Apochromat 40x 1,1 Water 2,1 / 2,3 1,3 / 1,4 0,7 / 0,7


α
Plan-Apochromat 63x 1,46 Oil 1,5 / 1,0 0,9 / 0,6 0,5 / 0,3


α
Plan-Fluar 100x 1,45 Oil 1,0 / 0,7 0,6 / 0,4 0,3 / 0,2


α
Plan-Apochromat 100x 1,46 Oil 1,0 / 0,7 0,6 / 0,4 0,3 / 0,2



INSTRUMENT DESCRIPTION
ApoTome.2 Suitability of objectives for the use of ApoTome.2 Carl Zeiss
05/2012 423667-7144-001 19
Axio Zoom.V16
Objective M Zoom Grid / Section thickness
@ 490nm [RU/µm]
DAPI with
FS 34
DAPI with
FS 49
(total magnification) High grid Middle grid Low grid
Plan-Apochromat 1x 40 1,8 / 82,7 1,22 / 56,7 0,9 / 43,8


Plan-Apochromat 1x 80 1,5 / 25,2 1,0 / 17,4 0,8 / 13,5


Plan-Apochromat 1x 100 1,2 / 19,7 0,9 / 13,7 0,7 / 10,8


Plan-Apochromat 1x 112 1,1 / 17,7 0,8 / 12,4 0,6 / 9,8


Plan-Neofluar 1x 40 1,8 / 82,7 1,22 / 56,7 0,9 / 43,8


Plan-Neofluar 1x 80 1,5 / 25,2 1,0 / 17,4 0,8 / 13,5


Plan-Neofluar 1x 100 1,2 / 19,7 0,9 / 13,7 0,7 / 10,8


Plan-Neofluar 1x 112 1,1 / 17,7 0,8 / 12,4 0,6 / 9,8


Plan-Neofluar 2,3x 40 2,0 / 72,0 1,4 / 49,3 1,1 / 37,9


Plan-Neofluar 2,3x 12011,7 / 9,1 1,1 / 6,3 0,9 / 4,9


Plan-Neofluar 2,3x 150 1,6 / 5,9 1,1 / 4,1 0,8 / 3,1


Plan-Neofluar 2,3x 160 1,5 / 5,3 1,0 / 3,6 0,8 / 2,8


Plan-Neofluar 2,3x 250 1,1 / 3,2 0,7 / 2,2 0,6 / 1,8


1 In ZEN (blue edition) no preset zoom positions
The following filter sets are recommended if ApoTome.2 shall be used with Axio Zoom.V16:
20, 38, 43.
Axio Zoom.V16 allows the user to select also the zoom position, besides the objective.
ApoTome.2 may be used with Axio Zoom.V16 with a total magnification of 40x or higher.
Further filter sets recommended for ApoTome.2
Order No. Name Excitation / Color splitter / Emission Fluorophores (examples)
488001-9901-000 FS 01
BP 365/12
FT 395
LP 397
Alexa 405, BFP, DAPI
488802-9901-000 FS 02
G 365
FT 395
LP 420
Alexa 350, BFP, DAPI
488009-9901-000 FS 09
BP 450-490
FT 510
LP 515
Alexa 430, Alexa 488, FITC
488010-9901-000 FS 10
BP 450-490
FT 510
BP 515-565
Alexa 430, Alexa 488, FITC, Cy2
488016-9901-000 FS 16
BP 485/20
FT 510
LP 515
Alexa 488, FITC, MitoTracker Green
488017-9901-000 FS 17
BP 485/20
FT 510
BP 515-565
Alexa 488, FITC, MitoTracker Green, Cy2
488020-9901-000 FS 20
BP 546/12
FT 560
BP 575/640
Alexa 555, Rhodamine, dTomato

INSTRUMENT DESCRIPTION
Carl Zeiss Suitability of objectives for the use of ApoTome.2 ApoTome.2
20 423667-7144-001 05/2012
Order No. Name Excitation / Color splitter / Emission Fluorophores (examples)
488026-9901-000 FS 26
BP 575-625
FT 645
BP 660-710
mPlum, Nile Red
489038-9901-000 FS 38 HE
BP 470/40
FT 495
BP 525/50
Alexa 430, Alexa 488, FITC
489043-9901-000 FS 43 HE
BP 550/25 (HE)
FT 570 (HE)
BP 605/70 (HE)
Alexa 546, Alexa 555, Cy3, DiI
489046-9901-000 FS 46 HE
BP 500/25
FT 515
BP 535/30
Alexa 488, FITC, eGFP, eYFP
489047-9901-000 FS 47 HE
BP 436/25
FT 455 HE
BP 480/40
CFP
488049-9901-000 FS 49
G 365
FT 395
BP 445/50
DAPI
488050-9901-000 FS 50
BP 640/30
FT 660
BP 690/50
Alexa 647, Alexa 660, Cy5
489059-9901-000 FS 59 HE
(Colibri)
BP 445/25 (HE)
BP 510/15 (HE)
DFT 460 + 520 (HE)
DBP 480/22 + LP 530 (HE)
Alexa 433, Alexa 488, CFP, FITC, eYFP
489060-9901-000 FS 60
(Colibri)
BP 445/25 (HE)
BP 510/15 (HE)
BP 588/27 (HE)
TFT 460 + 520 + 605 (HE)
DBP 480/22 + 546/32 + LP 615
Álexa 430, Alexa 568, Alexa 594
489061-9901-000 FS 61
(Colibri)
BP 474/28 (HE)
BP 585/35 (HE)
DFT 495 + 605 (HE)
DBP 527/54 + 645/60 (HE)
CFP, YFP, mRFP
489062-9901-000 FS 62
(Colibri)
BP 370/40 (HE)
BP 474/28 (HE)
BP 585/35 (HE)
TFT 395 + 495 + 610 (HE)
TBP 425 + 527 + LP615 (HE)
DAPI, CFP, YFP